LOCUS       DANKBF010000001       148577 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000001 DANKBF010000000
VERSION     DANKBF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 148577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 148577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..148577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="PWY89_000001"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="PWY89_000001"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2065073.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="PWY89_000002"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="PWY89_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL2065074.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="PWY89_000003"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="PWY89_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2065075.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="PWY89_000004"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="PWY89_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2065076.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="PWY89_000005"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="PWY89_000005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2065077.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="PWY89_000006"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="PWY89_000006"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2065078.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="PWY89_000007"
     CDS             complement(8557..9027)
                     /locus_tag="PWY89_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL2065079.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="PWY89_000008"
     CDS             complement(9073..9498)
                     /locus_tag="PWY89_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL2065080.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="PWY89_000009"
     CDS             complement(9565..9834)
                     /locus_tag="PWY89_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL2065081.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="PWY89_000010"
     CDS             complement(9845..11440)
                     /locus_tag="PWY89_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2065082.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="PWY89_000011"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="PWY89_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2065083.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="PWY89_000012"
     CDS             complement(14993..15553)
                     /locus_tag="PWY89_000012"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2065084.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="PWY89_000013"
     CDS             15906..16880
                     /locus_tag="PWY89_000013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2065085.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="PWY89_000014"
     CDS             17050..18450
                     /locus_tag="PWY89_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL2065086.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="PWY89_000015"
     CDS             complement(18528..19250)
                     /locus_tag="PWY89_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2065087.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="PWY89_000016"
     CDS             complement(19329..19835)
                     /locus_tag="PWY89_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2065088.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="PWY89_000017"
     CDS             19958..21757
                     /locus_tag="PWY89_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL2065089.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..23435)
                     /locus_tag="PWY89_000018"
     CDS             complement(21825..23435)
                     /locus_tag="PWY89_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2065090.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTG
                     SSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCS
                     GFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGG
                     QYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(23690..24199)
                     /gene="mreD"
                     /locus_tag="PWY89_000019"
     CDS             complement(23690..24199)
                     /gene="mreD"
                     /locus_tag="PWY89_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2065091.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24203..25057)
                     /gene="mreC"
                     /locus_tag="PWY89_000020"
     CDS             complement(24203..25057)
                     /gene="mreC"
                     /locus_tag="PWY89_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2065092.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25212..25850)
                     /locus_tag="PWY89_000021"
     CDS             complement(25212..25850)
                     /locus_tag="PWY89_000021"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL2065093.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25851..26501)
                     /locus_tag="PWY89_000022"
     CDS             complement(25851..26501)
                     /locus_tag="PWY89_000022"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2065094.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26514..27413)
                     /gene="rsgA"
                     /locus_tag="PWY89_000023"
     CDS             complement(26514..27413)
                     /gene="rsgA"
                     /locus_tag="PWY89_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL2065095.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27578..29647)
                     /gene="pknB"
                     /locus_tag="PWY89_000024"
     CDS             complement(27578..29647)
                     /gene="pknB"
                     /locus_tag="PWY89_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL2065096.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29644..30384)
                     /locus_tag="PWY89_000025"
     CDS             complement(29644..30384)
                     /locus_tag="PWY89_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL2065097.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30397..31755)
                     /gene="rsmB"
                     /locus_tag="PWY89_000026"
     CDS             complement(30397..31755)
                     /gene="rsmB"
                     /locus_tag="PWY89_000026"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2065098.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31755..32693)
                     /locus_tag="PWY89_000027"
     CDS             complement(31755..32693)
                     /locus_tag="PWY89_000027"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2065099.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32686..33177)
                     /gene="def"
                     /locus_tag="PWY89_000028"
     CDS             complement(32686..33177)
                     /gene="def"
                     /locus_tag="PWY89_000028"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2065100.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33192..35600)
                     /gene="priA"
                     /locus_tag="PWY89_000029"
     CDS             complement(33192..35600)
                     /gene="priA"
                     /locus_tag="PWY89_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2065101.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35853..36158)
                     /locus_tag="PWY89_000030"
     CDS             complement(35853..36158)
                     /locus_tag="PWY89_000030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2065102.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36163..36777)
                     /gene="gmk"
                     /locus_tag="PWY89_000031"
     CDS             complement(36163..36777)
                     /gene="gmk"
                     /locus_tag="PWY89_000031"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2065103.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37264..37548)
                     /locus_tag="PWY89_000032"
     CDS             complement(37264..37548)
                     /locus_tag="PWY89_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065104.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37566..38456)
                     /locus_tag="PWY89_000033"
     CDS             complement(37566..38456)
                     /locus_tag="PWY89_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2065105.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38476..38694)
                     /locus_tag="PWY89_000034"
     CDS             complement(38476..38694)
                     /locus_tag="PWY89_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL2065106.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38664..40364)
                     /locus_tag="PWY89_000035"
     CDS             complement(38664..40364)
                     /locus_tag="PWY89_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2065107.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40631..41404
                     /locus_tag="PWY89_000036"
     CDS             40631..41404
                     /locus_tag="PWY89_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065108.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41485..42054)
                     /gene="thiT"
                     /locus_tag="PWY89_000037"
     CDS             complement(41485..42054)
                     /gene="thiT"
                     /locus_tag="PWY89_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL2065109.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(42120..42222)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(42228..43199)
                     /locus_tag="PWY89_000038"
     CDS             complement(42228..43199)
                     /locus_tag="PWY89_000038"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2065110.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(43293..44666)
                     /gene="glmU"
                     /locus_tag="PWY89_000039"
     CDS             complement(43293..44666)
                     /gene="glmU"
                     /locus_tag="PWY89_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2065111.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(45169..45987)
                     /gene="purR"
                     /locus_tag="PWY89_000040"
     CDS             complement(45169..45987)
                     /gene="purR"
                     /locus_tag="PWY89_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL2065112.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(46154..46978)
                     /locus_tag="PWY89_000041"
     CDS             complement(46154..46978)
                     /locus_tag="PWY89_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2065113.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46951..47631)
                     /locus_tag="PWY89_000042"
     CDS             complement(46951..47631)
                     /locus_tag="PWY89_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2065114.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47654..48592)
                     /locus_tag="PWY89_000043"
     CDS             complement(47654..48592)
                     /locus_tag="PWY89_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2065115.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48837..49700)
                     /locus_tag="PWY89_000044"
     CDS             complement(48837..49700)
                     /locus_tag="PWY89_000044"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2065116.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49842..52415)
                     /gene="mprF"
                     /locus_tag="PWY89_000045"
     CDS             complement(49842..52415)
                     /gene="mprF"
                     /locus_tag="PWY89_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2065117.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52556..53362
                     /gene="proC"
                     /locus_tag="PWY89_000046"
     CDS             52556..53362
                     /gene="proC"
                     /locus_tag="PWY89_000046"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2065118.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(53441..54997)
                     /gene="rny"
                     /locus_tag="PWY89_000047"
     CDS             complement(53441..54997)
                     /gene="rny"
                     /locus_tag="PWY89_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL2065119.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(55408..56457)
                     /gene="recA"
                     /locus_tag="PWY89_000048"
     CDS             complement(55408..56457)
                     /gene="recA"
                     /locus_tag="PWY89_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2065120.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56566..57807)
                     /locus_tag="PWY89_000049"
     CDS             complement(56566..57807)
                     /locus_tag="PWY89_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL2065121.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(58047..58625)
                     /gene="pgsA"
                     /locus_tag="PWY89_000050"
     CDS             complement(58047..58625)
                     /gene="pgsA"
                     /locus_tag="PWY89_000050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2065122.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58834..59757)
                     /locus_tag="PWY89_000051"
     CDS             complement(58834..59757)
                     /locus_tag="PWY89_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065123.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(60496..61782)
                     /locus_tag="PWY89_000052"
     CDS             complement(60496..61782)
                     /locus_tag="PWY89_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2065124.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61775..63043)
                     /locus_tag="PWY89_000053"
     CDS             complement(61775..63043)
                     /locus_tag="PWY89_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2065125.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            63295..63756
                     /gene="mscL"
                     /locus_tag="PWY89_000054"
     CDS             63295..63756
                     /gene="mscL"
                     /locus_tag="PWY89_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2065126.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63815..64261)
                     /locus_tag="PWY89_000055"
     CDS             complement(63815..64261)
                     /locus_tag="PWY89_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2065127.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(64354..64599)
                     /locus_tag="PWY89_000056"
     CDS             complement(64354..64599)
                     /locus_tag="PWY89_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL2065128.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64714..65292)
                     /locus_tag="PWY89_000057"
     CDS             complement(64714..65292)
                     /locus_tag="PWY89_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2065129.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(65369..65899)
                     /locus_tag="PWY89_000058"
     CDS             complement(65369..65899)
                     /locus_tag="PWY89_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL2065130.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65903..66763)
                     /gene="rlmB"
                     /locus_tag="PWY89_000059"
     CDS             complement(65903..66763)
                     /gene="rlmB"
                     /locus_tag="PWY89_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2065131.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66756..67151)
                     /locus_tag="PWY89_000060"
     CDS             complement(66756..67151)
                     /locus_tag="PWY89_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL2065132.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(67148..68560)
                     /locus_tag="PWY89_000061"
     CDS             complement(67148..68560)
                     /locus_tag="PWY89_000061"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2065133.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68557..69093)
                     /gene="cysE"
                     /locus_tag="PWY89_000062"
     CDS             complement(68557..69093)
                     /gene="cysE"
                     /locus_tag="PWY89_000062"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2065134.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(69179..69418)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(69415..70875)
                     /locus_tag="PWY89_000063"
     CDS             complement(69415..70875)
                     /locus_tag="PWY89_000063"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2065135.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(71045..72202)
                     /locus_tag="PWY89_000064"
     CDS             complement(71045..72202)
                     /locus_tag="PWY89_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL2065136.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(72236..73606)
                     /gene="radA"
                     /locus_tag="PWY89_000065"
     CDS             complement(72236..73606)
                     /gene="radA"
                     /locus_tag="PWY89_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2065137.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73669..74196)
                     /locus_tag="PWY89_000066"
     CDS             complement(73669..74196)
                     /locus_tag="PWY89_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2065138.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            74426..75232
                     /gene="proB"
                     /locus_tag="PWY89_000067"
     CDS             74426..75232
                     /gene="proB"
                     /locus_tag="PWY89_000067"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2065139.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            75229..76476
                     /locus_tag="PWY89_000068"
     CDS             75229..76476
                     /locus_tag="PWY89_000068"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2065140.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76552..76896
                     /locus_tag="PWY89_000069"
     CDS             76552..76896
                     /locus_tag="PWY89_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065141.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(77013..77339)
                     /locus_tag="PWY89_000070"
     CDS             complement(77013..77339)
                     /locus_tag="PWY89_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065142.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77954..79156)
                     /locus_tag="PWY89_000071"
     CDS             complement(77954..79156)
                     /locus_tag="PWY89_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2065143.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            79273..79788
                     /locus_tag="PWY89_000072"
     CDS             79273..79788
                     /locus_tag="PWY89_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2065144.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79987..80433)
                     /locus_tag="PWY89_000073"
     CDS             complement(79987..80433)
                     /locus_tag="PWY89_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2065145.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(80480..81958)
                     /gene="msr(C)"
                     /locus_tag="PWY89_000074"
     CDS             complement(80480..81958)
                     /gene="msr(C)"
                     /locus_tag="PWY89_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2065146.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(82312..82941)
                     /locus_tag="PWY89_000075"
     CDS             complement(82312..82941)
                     /locus_tag="PWY89_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2065147.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82925..83389)
                     /locus_tag="PWY89_000076"
     CDS             complement(82925..83389)
                     /locus_tag="PWY89_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2065148.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83876..85144)
                     /locus_tag="PWY89_000077"
     CDS             complement(83876..85144)
                     /locus_tag="PWY89_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2065149.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(85375..86112)
                     /locus_tag="PWY89_000078"
     CDS             complement(85375..86112)
                     /locus_tag="PWY89_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2065150.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(86179..86748)
                     /locus_tag="PWY89_000079"
     CDS             complement(86179..86748)
                     /locus_tag="PWY89_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2065151.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86876..87748
                     /locus_tag="PWY89_000080"
     CDS             86876..87748
                     /locus_tag="PWY89_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2065152.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            88090..88962
                     /locus_tag="PWY89_000081"
     CDS             88090..88962
                     /locus_tag="PWY89_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2065153.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88959..90917
                     /locus_tag="PWY89_000082"
     CDS             88959..90917
                     /locus_tag="PWY89_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2065154.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(91249..92100)
                     /locus_tag="PWY89_000083"
     CDS             complement(91249..92100)
                     /locus_tag="PWY89_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2065155.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            92223..92690
                     /locus_tag="PWY89_000084"
     CDS             92223..92690
                     /locus_tag="PWY89_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2065156.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92953..93435)
                     /locus_tag="PWY89_000085"
     CDS             complement(92953..93435)
                     /locus_tag="PWY89_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2065157.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(93535..94398)
                     /locus_tag="PWY89_000086"
     CDS             complement(93535..94398)
                     /locus_tag="PWY89_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2065158.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            94996..95871
                     /locus_tag="PWY89_000087"
     CDS             94996..95871
                     /locus_tag="PWY89_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065159.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(96016..96168)
                     /locus_tag="PWY89_000088"
                     /pseudo
     CDS             complement(96016..96168)
                     /locus_tag="PWY89_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(96436..97767)
                     /locus_tag="PWY89_000089"
     CDS             complement(96436..97767)
                     /locus_tag="PWY89_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2065160.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(97917..98630)
                     /locus_tag="PWY89_000090"
     CDS             complement(97917..98630)
                     /locus_tag="PWY89_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2065161.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            98805..100229
                     /locus_tag="PWY89_000091"
     CDS             98805..100229
                     /locus_tag="PWY89_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2065162.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            100528..101235
                     /locus_tag="PWY89_000092"
     CDS             100528..101235
                     /locus_tag="PWY89_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2065163.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            101222..101545
                     /locus_tag="PWY89_000093"
     CDS             101222..101545
                     /locus_tag="PWY89_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2065164.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(101634..102671)
                     /locus_tag="PWY89_000094"
     CDS             complement(101634..102671)
                     /locus_tag="PWY89_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2065165.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(102992..103975)
                     /locus_tag="PWY89_000095"
     CDS             complement(102992..103975)
                     /locus_tag="PWY89_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2065166.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(104390..105160)
                     /locus_tag="PWY89_000096"
     CDS             complement(104390..105160)
                     /locus_tag="PWY89_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065167.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(105153..106088)
                     /locus_tag="PWY89_000097"
     CDS             complement(105153..106088)
                     /locus_tag="PWY89_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065168.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(106404..108053)
                     /locus_tag="PWY89_000098"
     CDS             complement(106404..108053)
                     /locus_tag="PWY89_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2065169.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(108170..110773)
                     /locus_tag="PWY89_000099"
     CDS             complement(108170..110773)
                     /locus_tag="PWY89_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2065170.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(110785..111834)
                     /locus_tag="PWY89_000100"
     CDS             complement(110785..111834)
                     /locus_tag="PWY89_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL2065171.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(111860..112174)
                     /locus_tag="PWY89_000101"
     CDS             complement(111860..112174)
                     /locus_tag="PWY89_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL2065172.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(112190..112636)
                     /locus_tag="PWY89_000102"
     CDS             complement(112190..112636)
                     /locus_tag="PWY89_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2065173.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(112637..114559)
                     /locus_tag="PWY89_000103"
     CDS             complement(112637..114559)
                     /locus_tag="PWY89_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL2065174.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(114771..115667)
                     /locus_tag="PWY89_000104"
     CDS             complement(114771..115667)
                     /locus_tag="PWY89_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2065175.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(115660..116340)
                     /locus_tag="PWY89_000105"
     CDS             complement(115660..116340)
                     /locus_tag="PWY89_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2065176.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(116341..117123)
                     /locus_tag="PWY89_000106"
     CDS             complement(116341..117123)
                     /locus_tag="PWY89_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065177.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(117116..118033)
                     /locus_tag="PWY89_000107"
     CDS             complement(117116..118033)
                     /locus_tag="PWY89_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2065178.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(118197..118520)
                     /locus_tag="PWY89_000108"
     CDS             complement(118197..118520)
                     /locus_tag="PWY89_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065179.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            119308..122535
                     /gene="fss3"
                     /locus_tag="PWY89_000109"
     CDS             119308..122535
                     /gene="fss3"
                     /locus_tag="PWY89_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2065180.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            122648..122962
                     /locus_tag="PWY89_000110"
     CDS             122648..122962
                     /locus_tag="PWY89_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2065181.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            122975..123349
                     /locus_tag="PWY89_000111"
     CDS             122975..123349
                     /locus_tag="PWY89_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2065182.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            123359..123694
                     /locus_tag="PWY89_000112"
     CDS             123359..123694
                     /locus_tag="PWY89_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL2065183.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            123777..125117
                     /locus_tag="PWY89_000113"
     CDS             123777..125117
                     /locus_tag="PWY89_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2065184.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            125195..125869
                     /locus_tag="PWY89_000114"
     CDS             125195..125869
                     /locus_tag="PWY89_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2065185.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            125862..126026
                     /locus_tag="PWY89_000115"
     CDS             125862..126026
                     /locus_tag="PWY89_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065186.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            126102..127244
                     /gene="dcm"
                     /locus_tag="PWY89_000116"
                     /pseudo
     CDS             126102..127244
                     /gene="dcm"
                     /locus_tag="PWY89_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            127234..127524
                     /locus_tag="PWY89_000117"
     CDS             127234..127524
                     /locus_tag="PWY89_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065187.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            127783..128967
                     /locus_tag="PWY89_000118"
     CDS             127783..128967
                     /locus_tag="PWY89_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2065188.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            128964..129101
                     /locus_tag="PWY89_000119"
     CDS             128964..129101
                     /locus_tag="PWY89_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2065189.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            129847..131757
                     /gene="ltrA"
                     /locus_tag="PWY89_000120"
     CDS             129847..131757
                     /gene="ltrA"
                     /locus_tag="PWY89_000120"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2065190.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            131861..132085
                     /locus_tag="PWY89_000121"
     CDS             131861..132085
                     /locus_tag="PWY89_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065191.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            132098..132601
                     /locus_tag="PWY89_000122"
     CDS             132098..132601
                     /locus_tag="PWY89_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2065192.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            132661..133050
                     /locus_tag="PWY89_000123"
     CDS             132661..133050
                     /locus_tag="PWY89_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2065193.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            133037..135484
                     /locus_tag="PWY89_000124"
     CDS             133037..135484
                     /locus_tag="PWY89_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2065194.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            135489..137612
                     /locus_tag="PWY89_000125"
     CDS             135489..137612
                     /locus_tag="PWY89_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2065195.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            137609..138613
                     /locus_tag="PWY89_000126"
     CDS             137609..138613
                     /locus_tag="PWY89_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2065196.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            138632..139543
                     /locus_tag="PWY89_000127"
     CDS             138632..139543
                     /locus_tag="PWY89_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2065197.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            139730..139986
                     /locus_tag="PWY89_000128"
                     /pseudo
     CDS             139730..139986
                     /locus_tag="PWY89_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(140203..140418)
                     /locus_tag="PWY89_000129"
     CDS             complement(140203..140418)
                     /locus_tag="PWY89_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065198.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(140667..140885)
                     /locus_tag="PWY89_000130"
                     /pseudo
     CDS             complement(140667..140885)
                     /locus_tag="PWY89_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(141180..142619)
                     /locus_tag="PWY89_000131"
     CDS             complement(141180..142619)
                     /locus_tag="PWY89_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2065199.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            complement(143207..143839)
                     /locus_tag="PWY89_000132"
     CDS             complement(143207..143839)
                     /locus_tag="PWY89_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL2065200.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            complement(144188..145639)
                     /locus_tag="PWY89_000133"
     CDS             complement(144188..145639)
                     /locus_tag="PWY89_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2065201.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(145751..147094)
                     /locus_tag="PWY89_000134"
     CDS             complement(145751..147094)
                     /locus_tag="PWY89_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2065202.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(147228..>148577)
                     /locus_tag="PWY89_000135"
     CDS             complement(147228..>148577)
                     /locus_tag="PWY89_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2065203.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT        43353 a        30504 c        26942 g        47778 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc actgttgttg attcttctgt tgctgaagat
    22381 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    22441 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    22501 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22561 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22621 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22681 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22741 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22801 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22861 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22921 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22981 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    23041 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    23101 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    23161 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    23221 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    23281 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    23341 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    23401 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    23461 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    23521 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23581 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23641 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23701 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23761 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23821 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23881 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23941 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    24001 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    24061 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    24121 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    24181 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    24241 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    24301 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    24361 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    24421 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    24481 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24541 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24601 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24661 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24721 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24781 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24841 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24901 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24961 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    25021 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    25081 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    25141 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    25201 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    25261 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    25321 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    25381 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    25441 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    25501 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25561 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25621 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25681 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25741 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25801 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25861 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25921 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25981 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    26041 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    26101 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    26161 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    26221 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    26281 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    26341 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    26401 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    26461 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    26521 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26581 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26641 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26701 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26761 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26821 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26881 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26941 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    27001 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    27061 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    27121 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    27181 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    27241 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    27301 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    27361 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    27421 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    27481 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27541 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27601 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27661 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27721 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27781 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27841 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27901 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27961 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    28021 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    28081 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    28141 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    28201 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    28261 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    28321 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    28381 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    28441 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    28501 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28561 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28621 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28681 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28741 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28801 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28861 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28921 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28981 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    29041 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    29101 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    29161 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    29221 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    29281 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    29341 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    29401 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    29461 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    29521 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29581 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29641 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29701 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29761 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29821 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29881 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29941 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    30001 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    30061 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    30121 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    30181 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    30241 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    30301 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    30361 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    30421 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    30481 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30541 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30601 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30661 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30721 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30781 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30841 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30901 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30961 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    31021 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    31081 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    31141 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    31201 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    31261 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    31321 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    31381 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    31441 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    31501 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31561 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31621 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31681 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31741 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31801 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31861 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31921 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31981 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    32041 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    32101 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    32161 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    32221 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    32281 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    32341 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    32401 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    32461 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    32521 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32581 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32641 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32701 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32761 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32821 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32881 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32941 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    33001 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    33061 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    33121 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    33181 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    33241 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    33301 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    33361 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    33421 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    33481 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33541 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33601 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33661 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33721 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33781 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33841 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33901 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33961 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    34021 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    34081 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    34141 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    34201 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    34261 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    34321 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    34381 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    34441 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    34501 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34561 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34621 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34681 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34741 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34801 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34861 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34921 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34981 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    35041 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    35101 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    35161 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    35221 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    35281 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    35341 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    35401 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    35461 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    35521 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35581 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35641 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35701 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35761 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35821 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35881 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35941 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    36001 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    36061 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    36121 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    36181 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    36241 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    36301 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    36361 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    36421 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    36481 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36541 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36601 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36661 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36721 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36781 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36841 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36901 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36961 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    37021 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    37081 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    37141 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    37201 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    37261 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    37321 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    37381 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    37441 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    37501 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37561 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37621 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37681 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37741 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37801 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37861 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37921 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37981 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    38041 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    38101 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    38161 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    38221 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    38281 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    38341 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    38401 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    38461 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    38521 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38581 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38641 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38701 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38761 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38821 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38881 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38941 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    39001 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    39061 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    39121 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    39181 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    39241 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    39301 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    39361 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    39421 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    39481 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39541 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39601 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39661 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39721 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39781 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39841 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39901 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39961 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    40021 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    40081 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    40141 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    40201 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    40261 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    40321 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    40381 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    40441 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    40501 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40561 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40621 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40681 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40741 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40801 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40861 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40921 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40981 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    41041 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    41101 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    41161 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    41221 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    41281 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    41341 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    41401 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    41461 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    41521 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41581 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41641 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41701 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41761 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41821 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41881 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41941 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    42001 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    42061 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    42121 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    42181 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    42241 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    42301 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    42361 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    42421 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    42481 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42541 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42601 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42661 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42721 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42781 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42841 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42901 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42961 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    43021 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    43081 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    43141 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    43201 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    43261 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    43321 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    43381 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    43441 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    43501 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43561 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43621 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43681 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43741 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43801 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43861 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43921 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43981 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    44041 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    44101 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    44161 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    44221 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    44281 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    44341 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    44401 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    44461 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    44521 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44581 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44641 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44701 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44761 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44821 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44881 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44941 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    45001 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    45061 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    45121 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    45181 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    45241 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    45301 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    45361 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    45421 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    45481 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45541 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45601 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45661 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45721 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45781 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45841 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45901 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45961 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    46021 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    46081 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    46141 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    46201 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    46261 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    46321 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    46381 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    46441 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    46501 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46561 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46621 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46681 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46741 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46801 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46861 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46921 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46981 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    47041 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    47101 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    47161 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    47221 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    47281 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    47341 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    47401 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    47461 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    47521 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47581 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47641 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47701 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47761 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47821 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47881 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47941 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    48001 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    48061 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    48121 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    48181 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    48241 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    48301 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    48361 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    48421 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    48481 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48541 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48601 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48661 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48721 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48781 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48841 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48901 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48961 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    49021 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    49081 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    49141 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    49201 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    49261 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    49321 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    49381 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    49441 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    49501 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49561 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49621 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49681 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49741 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49801 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49861 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49921 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49981 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    50041 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    50101 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    50161 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    50221 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    50281 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    50341 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    50401 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    50461 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    50521 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50581 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50641 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50701 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50761 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50821 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50881 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50941 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    51001 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    51061 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    51121 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    51181 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    51241 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    51301 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    51361 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    51421 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    51481 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51541 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51601 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51661 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51721 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51781 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51841 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51901 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51961 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    52021 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    52081 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    52141 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    52201 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    52261 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    52321 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    52381 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    52441 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    52501 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52561 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52621 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52681 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52741 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52801 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52861 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52921 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52981 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    53041 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    53101 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    53161 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    53221 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    53281 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    53341 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    53401 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    53461 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    53521 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53581 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53641 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53701 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53761 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53821 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53881 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53941 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    54001 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    54061 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    54121 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    54181 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    54241 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    54301 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    54361 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    54421 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    54481 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54541 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54601 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54661 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54721 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54781 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54841 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54901 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54961 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    55021 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    55081 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    55141 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    55201 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    55261 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    55321 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    55381 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    55441 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    55501 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55561 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55621 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55681 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55741 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55801 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcg caaacatcac
    55861 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55921 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55981 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    56041 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    56101 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    56161 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    56221 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    56281 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    56341 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    56401 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    56461 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    56521 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56581 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56641 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56701 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56761 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56821 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56881 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56941 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    57001 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    57061 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    57121 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    57181 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    57241 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    57301 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    57361 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    57421 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    57481 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57541 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57601 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57661 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57721 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57781 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57841 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57901 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57961 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    58021 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    58081 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    58141 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    58201 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    58261 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    58321 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    58381 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    58441 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    58501 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58561 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58621 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58681 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58741 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58801 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58861 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58921 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58981 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    59041 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    59101 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    59161 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    59221 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    59281 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    59341 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    59401 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    59461 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    59521 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59581 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59641 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59701 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59761 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59821 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59881 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59941 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    60001 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    60061 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    60121 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    60181 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    60241 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    60301 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    60361 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    60421 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    60481 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60541 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60601 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60661 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60721 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60781 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60841 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60901 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60961 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    61021 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    61081 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    61141 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    61201 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    61261 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    61321 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    61381 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    61441 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    61501 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61561 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61621 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61681 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61741 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61801 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61861 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61921 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61981 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    62041 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    62101 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    62161 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    62221 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    62281 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    62341 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    62401 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    62461 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    62521 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62581 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62641 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62701 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62761 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62821 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62881 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62941 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    63001 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    63061 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    63121 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    63181 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    63241 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    63301 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    63361 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    63421 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    63481 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63541 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63601 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63661 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63721 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaagaaagc
    63781 cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt tcctttttca
    63841 tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg aatccaaacg
    63901 actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt tctggaaaat
    63961 ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca tctttacggt
    64021 tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca tatactcgcg
    64081 tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct tctccatcaa
    64141 tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg attcgttctt
    64201 tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc tgatattcca
    64261 ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac aaaaaagcaa
    64321 acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta aaaattcgat
    64381 ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa aagaattttc
    64441 ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa cgccgcggcg
    64501 ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc cgatacgacc
    64561 ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct ctactatcaa
    64621 ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa atgcaataaa
    64681 aataaataag aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg
    64741 tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc
    64801 taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc
    64861 ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg
    64921 aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat
    64981 tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct
    65041 caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa agctaacacc
    65101 tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca
    65161 atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa
    65221 atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa
    65281 ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat
    65341 gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt
    65401 tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa
    65461 aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata
    65521 attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat
    65581 cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga
    65641 tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat
    65701 aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt
    65761 catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt
    65821 cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa
    65881 tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa
    65941 gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca
    66001 gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga
    66061 ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc
    66121 caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc
    66181 gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc
    66241 caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta
    66301 aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt
    66361 cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa
    66421 tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag
    66481 gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt
    66541 ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta
    66601 ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa
    66661 ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa
    66721 atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc
    66781 tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag
    66841 cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg
    66901 ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa
    66961 agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc
    67021 gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga
    67081 acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg
    67141 taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc
    67201 ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg
    67261 cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa
    67321 ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag
    67381 cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca
    67441 ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta
    67501 aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt
    67561 ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt
    67621 tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac
    67681 gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag
    67741 atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat
    67801 tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa
    67861 attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca
    67921 taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa
    67981 tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct
    68041 gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta
    68101 atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag
    68161 catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga
    68221 ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc
    68281 ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga
    68341 ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa
    68401 aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat
    68461 tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt
    68521 cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc
    68581 tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc
    68641 tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa
    68701 tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt
    68761 ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc
    68821 tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt
    68881 tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc
    68941 caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg
    69001 aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt
    69061 tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt
    69121 tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc
    69181 aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct
    69241 aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct
    69301 gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc
    69361 acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt
    69421 cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg
    69481 caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt
    69541 tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc
    69601 tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc
    69661 accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt
    69721 agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc taacgatctt
    69781 cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag
    69841 ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt
    69901 tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc
    69961 gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc
    70021 caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc
    70081 gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat
    70141 gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag
    70201 ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa
    70261 atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt tttggatgac
    70321 aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa
    70381 tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc
    70441 tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc
    70501 tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt
    70561 gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc
    70621 agattgacgg taagggccgt attctccagg attttcagga gattcatccc aattcattcc
    70681 caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc
    70741 agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa
    70801 taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac
    70861 gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat
    70921 tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc
    70981 ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc
    71041 ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc
    71101 ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta
    71161 caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc
    71221 cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat
    71281 tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa
    71341 gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga
    71401 caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa
    71461 agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat
    71521 ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta
    71581 gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa
    71641 tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat
    71701 tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga
    71761 tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc
    71821 tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg
    71881 gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg
    71941 caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt tgttctactt
    72001 gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg
    72061 cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg
    72121 cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca
    72181 aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc
    72241 ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg
    72301 gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt
    72361 ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa
    72421 tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac
    72481 tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct gcagctttta
    72541 agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca
    72601 gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag
    72661 gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga
    72721 tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga
    72781 cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc
    72841 ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca
    72901 ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat
    72961 gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt tctgcagtgg
    73021 tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt
    73081 ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt
    73141 tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc
    73201 gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg
    73261 ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac
    73321 caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta
    73381 attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg
    73441 ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt
    73501 cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat
    73561 acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt
    73621 ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga
    73681 agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg
    73741 caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa
    73801 gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc
    73861 aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt
    73921 agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt
    73981 tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc
    74041 caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc
    74101 aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat tttgatcttt
    74161 atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta
    74221 ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt
    74281 ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa
    74341 aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg
    74401 agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat
    74461 tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat
    74521 cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt
    74581 ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt
    74641 gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa
    74701 tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt
    74761 agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga
    74821 cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac
    74881 aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct
    74941 tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga
    75001 ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg
    75061 tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat
    75121 atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga
    75181 tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg
    75241 atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac
    75301 cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc
    75361 attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg
    75421 caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa
    75481 attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga
    75541 ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt
    75601 ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt
    75661 cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta
    75721 ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa
    75781 actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga
    75841 gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca
    75901 ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc
    75961 gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat
    76021 tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc
    76081 tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac
    76141 acagattggg atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg
    76201 gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca
    76261 gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc
    76321 aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc
    76381 tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
    76441 tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc
    76501 gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa
    76561 attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc
    76621 ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaaa
    76681 tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt
    76741 tacatggttg cattatgctt attagcgaca atcaattact attctttttt cgatcattca
    76801 atcgcttata cgattacgct actgattgct gggatttttc acttatctta taaatatcag
    76861 ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag
    76921 tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc
    76981 acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc
    77041 tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca
    77101 tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt
    77161 tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct
    77221 tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa
    77281 aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc
    77341 tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa aattttccag
    77401 tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc
    77461 gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg
    77521 agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct
    77581 cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat
    77641 gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg attttacaac
    77701 ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata
    77761 tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg attttacaac
    77821 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    77881 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    77941 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    78001 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    78061 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    78121 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    78181 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    78241 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    78301 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    78361 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    78421 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    78481 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    78541 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    78601 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    78661 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    78721 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    78781 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    78841 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
    78901 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    78961 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    79021 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    79081 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    79141 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
    79201 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    79261 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    79321 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    79381 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    79441 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    79501 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    79561 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    79621 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    79681 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    79741 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    79801 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    79861 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    79921 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    79981 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    80041 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    80101 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    80161 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    80221 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    80281 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    80341 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    80401 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    80461 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    80521 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    80581 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    80641 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    80701 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    80761 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    80821 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    80881 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    80941 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    81001 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    81061 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    81121 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    81181 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
    81241 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    81301 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    81361 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    81421 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    81481 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    81541 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    81601 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    81661 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    81721 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    81781 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    81841 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    81901 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    81961 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    82021 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    82081 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    82141 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    82201 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    82261 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    82321 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    82381 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    82441 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    82501 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    82561 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    82621 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
    82681 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    82741 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
    82801 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    82861 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    82921 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    82981 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    83041 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
    83101 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    83161 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    83221 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    83281 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    83341 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    83401 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    83461 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    83521 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    83581 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
    83641 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    83701 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    83761 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    83821 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    83881 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    83941 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    84001 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    84061 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    84121 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    84181 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    84241 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    84301 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    84361 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    84421 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    84481 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    84541 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    84601 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    84661 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    84721 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    84781 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    84841 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    84901 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    84961 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
    85021 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    85081 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    85141 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    85201 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
    85261 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    85321 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
    85381 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
    85441 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
    85501 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    85561 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    85621 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    85681 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    85741 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    85801 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    85861 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    85921 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    85981 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    86041 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    86101 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    86161 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    86221 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    86281 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    86341 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    86401 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    86461 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    86521 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    86581 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    86641 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
    86701 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    86761 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
    86821 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    86881 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    86941 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    87001 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
    87061 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    87121 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    87181 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    87241 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    87301 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    87361 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
    87421 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    87481 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    87541 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    87601 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
    87661 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    87721 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    87781 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
    87841 tccttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    87901 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    87961 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    88021 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    88081 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    88141 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    88201 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    88261 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    88321 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    88381 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    88441 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    88501 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    88561 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    88621 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    88681 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    88741 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    88801 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    88861 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    88921 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    88981 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    89041 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    89101 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    89161 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    89221 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    89281 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    89341 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    89401 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    89461 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    89521 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    89581 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    89641 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    89701 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    89761 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
    89821 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    89881 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    89941 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    90001 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    90061 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    90121 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    90181 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    90241 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    90301 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    90361 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
    90421 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
    90481 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    90541 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    90601 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    90661 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    90721 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    90781 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    90841 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    90901 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    90961 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    91021 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
    91081 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
    91141 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
    91201 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    91261 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    91321 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    91381 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    91441 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
    91501 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    91561 ttttgctgca gcttctgcat attcacgttt caatacccaa cctgtttttc catttcctgc
    91621 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    91681 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    91741 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    91801 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    91861 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    91921 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    91981 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    92041 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    92101 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    92161 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    92221 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    92281 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    92341 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    92401 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    92461 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    92521 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    92581 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    92641 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    92701 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    92761 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    92821 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    92881 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    92941 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    93001 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    93061 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    93121 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
    93181 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    93241 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    93301 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    93361 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    93421 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    93481 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    93541 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    93601 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
    93661 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    93721 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    93781 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
    93841 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    93901 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    93961 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    94021 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    94081 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    94141 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    94201 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    94261 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    94321 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    94381 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
    94441 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    94501 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    94561 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
    94621 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    94681 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    94741 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    94801 aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct
    94861 ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat
    94921 gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca
    94981 tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc
    95041 ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg
    95101 tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc
    95161 aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct
    95221 gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc
    95281 tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt
    95341 atagaacatc cgcaatttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat
    95401 tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa
    95461 aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa
    95521 tcgctcattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct
    95581 actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac
    95641 tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg
    95701 ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactcc
    95761 ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata
    95821 gataaaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac
    95881 gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa aacacgaatc
    95941 atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat ttgactatta
    96001 ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctgggt agattttccg
    96061 gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt ccactgcatc
    96121 tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccact ctaatcttta
    96181 aagataaata ttaaaataac gattaataat attaacagtc tttatttttc accaccaaaa
    96241 acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc ttgtgaaaaa
    96301 taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt atcaaaaaat
    96361 atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg ttaatttttg
    96421 ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt gttcgtcaat
    96481 tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt gtaaaacagc
    96541 tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg tagtccaagg
    96601 aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg ctactgtcaa
    96661 agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata caataggcaa
    96721 tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata atttagcaaa
    96781 gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag caatcgtatt
    96841 tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa tatgtggaat
    96901 ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca aaaacggatc
    96961 taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata gattagcaat
    97021 agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg gctctgtaac
    97081 tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta taaatgacaa
    97141 taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag actttaaaac
    97201 agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag aaagtttaat
    97261 aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg aagctcctgt
    97321 ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt caggagtaaa
    97381 catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata ccgctgcaat
    97441 atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta caactaataa
    97501 tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta acgtggtcaa
    97561 tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca ctgaattaat
    97621 aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg catctcgcaa
    97681 agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt tttcaaatac
    97741 aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt gtaatcgatt
    97801 actttttcca cccaaataat acatcactta tcaaaaaaaa gtaaatactt ttaattaaat
    97861 atatatatct tttttagtat aaaaaaataa gctctgaaaa aataattttc ccgagcttat
    97921 ttttcaacaa aaacagcttc aaaaacaaag tcttgatata aatgtctcga taccgaatac
    97981 tcgaaaggtt taccagacga tagagcaaca actttgtcta tctcaatcat aaattcacct
    98041 ggatctattt ttaatatttt tgcgtcaaat tcattcgcag tatctccttt gatacgtatg
    98101 tgcgaagtct ttatttccag acccagttca tttgtgatat aagcatatac agatttcttt
    98161 aaatgttttg gttccaagcc tggtatgact gctaatggca tgaatgtttg ctcaatcgag
    98221 tatggttgac aatcaataaa acggcttcgt acaatattat atacatagtc ttttacactg
    98281 atacttaatt tatcagctac ttttttcgat ggaagttcaa ttgagaaagt atggatttct
    98341 gaatctacgt gttcttcacc aaccacgcta aacaaacctt cgtttggacc aagaactcgt
    98401 gagttatcaa ttggtgttct gacgacttca gtcccatacc cacttctact tcgcaaaact
    98461 ccttcggaaa caagtaagtc tacgcctttc tttactgtta acttgcttgt atgataaata
    98521 actgccaatt catctcctgt aggaagtttc tcaccaattt tatactctcc atttacaatt
    98581 tttttcttta tatctaagta aatttccata tacttcgcaa tttttttcaa tggtgaccct
    98641 ccaatcaaaa acatatcttc atctatactt taacataaat aaaccgataa aatccacatc
    98701 aaaattatac acacaataaa aaagatatat atatttaaac aaaactattt actttaagtt
    98761 gtaagcggtg ttatcattaa gtggaaacag gaggtaaaaa aattatgaca actatgaaaa
    98821 agctaacttt tccagatgat ttttggtggg gttccgcttg gtcagctgag caagcagaag
    98881 gaaaaggaga aacaggaaaa gcagagacta tctgggatcg ctggtttaaa gaacaacctt
    98941 accggtttta tcaacgaatc gattcctcta ttacaaccga tcacatccat caatataaag
    99001 aagatataga actaatgaaa aaaactgggc ataactcctt tcgcgtttct atttcttggg
    99061 cacgaatgtt tccaaatggc ggtaccggaa aagtaaatcc caaagccatt tctttctatc
    99121 gaaaattatt ttccgaaatg aataataacg gaatcaaagt atttgccaac ttatatcatt
    99181 ttgacatgcc tgctactctt caagataaag gaggatggga atcacgtgaa gtagttgatg
    99241 cttatgttca ttacgctgaa acttgtttta aagaatttgg tgacttagtt tgtcattggt
    99301 ttaccttcaa tgaaccactt gggcctattt taggtactta tcttgaagat tttcattatc
    99361 caaacctcat tgactttaaa cgcggagcgc aagcagcatt cttcactata cttgcacacg
    99421 caaaagcaat acaagtattt aaagaactag ctcttcctag taaaataggc gttattctaa
    99481 atttgagtcc tacttttcct cgtagtcaaa atcctgctga tgtgaaagca gcagaaatcg
    99541 ctgatttatt ttatacgcgg agctttttag atcctatggt taaagggaat ttttctaaaa
    99601 agcttatcct gttattacaa gaatataatc agcttcctga agattatacg aaagaagatc
    99661 ttgtatgtat ctacgaaaat accgctcaaa ttcttggact aaactattac gagccacgtc
    99721 gcgtcaaatc tcgttcttct atggtggata catctagtcc atttttacca gagtggtttt
    99781 tcgaaccaca tatcatgcct ggacgacgaa tgaacgaata ccggggatgg gaaatttacg
    99841 aaaaaggaat ttatgatttg tgcatggata tcaagaacca ttatggaaac atcgaagcat
    99901 ttatctcgga gaacggaatg ggcgttgcgg atgaagaacg tttcatggat gatacagggc
    99961 aaataattga tgattatcgg attgagtata tcaaagatca tcttgcttac ttatggaagg
   100021 caataaatga aggatgtaac attaaggggt atcatctttg gacatttatt gattgctggt
   100081 catggatcaa cgcctataaa aaccgatatg ggctggtttc actcgacctt actactcaaa
   100141 aaagaacaat taaaaaaagt ggcgaatttt acaagcaatt aagtgataca aatggattta
   100201 gatacgatgt agacttgtta gttgaataat attctaaaat tccacttgaa gtgaacacat
   100261 tagaaaacaa gaataatgag aaataccaat aacaaatact ttttcttcat acttcattta
   100321 tacttgtcag tataagagac tgtgacaaat gtagtgatcc tcagaagtta ggtctggaaa
   100381 tctaacttga gaagattttt ttaaactacg tccagtcttt tttatttgtt taaaattgaa
   100441 gttgacaagt cttcattaaa aatttagaat attcacaagt ttgacgacaa ttatgaaaat
   100501 ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt tagatataaa aactgcgatc
   100561 aaagacacgt tacctaccgt ttttggttat atcggtattg gacttgcatt tggtatcgtt
   100621 gggaaagctg ccgggtttca tccgctggtc gtcacgttga tgtccctgct gatctatgct
   100681 ggttctgccc aatttatcac ggtcagcatg cttgctagcc atagtccact gctctccatc
   100741 gttctctcga cctttctagt caattcccga atgattctga tgagtatgac gattgctcct
   100801 tatttcaaga aaaatcggct gcttcaaaat ctgctgattg gcacattgct gacagatgaa
   100861 agtttcgcac tgggaatgaa taaattgaac tatactggac aaaagttaaa ctttcgttgg
   100921 atgaatacag ctaattggat ttcttatttg acgtgggtgg gttcttctct tgttggcgca
   100981 cttttaggga attttattac tgatccgaaa aagtttggtt tggatttcgc catcgtcgct
   101041 atgtttatcg ggttacttta tcttcaagtc atttctgata gaaacacctc taaacgatta
   101101 cagttgatcc tgattggtct cactttgatt ctggtatatg tcggactgat ttttatccca
   101161 agtaacttag tgattttagt tgtaacactg attgggtgcg gattgggggt gtggatcaaa
   101221 catgccttct tttgagttta cattgctgac tattttaggt tgtactttag caacttggct
   101281 ttctaggatc ctaccatttg tcttactgaa aaaattcgat ttgccgcaac cgttgcttga
   101341 gtatttaagt tttgtcccga tcgtcattat gtctgcttta tggttcagca gtctatttac
   101401 ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat gctttggctt cagttcctac
   101461 acttttagct gctattcttt caaaaagctt attggtgatc gtcttggtag gaattctttc
   101521 cctttcgttg attcgcttat tataaataaa aaaacaagag cttatggcaa attactgtca
   101581 tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct tgcttatccg
   101641 ttgaacggac cactaacaac gacttttcct aatatatgtc ctgtttctac gtctttggtt
   101701 gcttgtttca agttcttcgc attcatccca tctgaataga ccttgctcaa tgtactgcgg
   101761 ataatccctt catttgctaa agcagcaatg tgcgctaaag cttctccttg cgtttcgatt
   101821 tcatagttat agtctgtttt agcgaacata tactcccagt cgaagctgat agattggttt
   101881 ttccaaacac ctatatctaa cggctctttg atccctacga tcgttcccac atgtccaaat
   101941 ggtcggacaa ggtgtttgat ttgatcaaag tagcgggtaa tatcgaataa tacagcgaca
   102001 tactctactt gatcgattcc taattcacga aaacttttac ttaagtcgtg atgataatcg
   102061 attggatagt ctacacctgt ttttttcagc cattcaaaat tcttcggact tgcagtcgcg
   102121 tagacttcta ggccagccca atgcgctagt tgattcaaaa tcgaaccgac gcccccagct
   102181 ccgttgatca ctaagatttt tttcccgcgg ttggcatttt cttctggagt cagaccaaat
   102241 ttttcaaata acaattcata agcggtcaat gaggtcaacg gcaaggctgc ggcttcttca
   102301 tctgaaagat tttttggtgc aagagcgaca atacgttcgt caaccagttg gtattcttga
   102361 ttgcttcctg ctcttgtcgt tgtacctgcg taaaaaacgc gatcgccgac agaaaatttt
   102421 tgcacttcat cacctacagc aacaactttc ccgactccat caaaccctaa gatacgtaaa
   102481 gcatttctta tcccgttatc ttggcgaagt ttcgtatcga caggattaac agatactgcg
   102541 ttgactttta ccagaagatc ccgtgaactg ggtacaggta tctgttcttt ttcatcaata
   102601 aagctttttg gatcatcgat cgacaaccct tcataaaaac cgactacctt cattaattcc
   102661 ttttctgaca tacgaattcc tccttagctt catcaagttg gagcatccat acagttttca
   102721 tttcaatata tatagtatgc tatttgttcc acttttattc atcataatta ttctcttact
   102781 taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga cttttgtcac
   102841 aacctctttt aaacggtgtt caatcaactg accctcggta ttcgctcaaa aaacgacaaa
   102901 atatgaggag ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac
   102961 gacctctaat tcacggtgtt caagcaagaa atcattgttc actgaaaagc gttggatgtt
   103021 ctttttcaaa ggtgtctaag atctctcgta cttcatcagt agatttcgta ttcatcaact
   103081 gaactcgcaa gtcatttgct cctggaaatc ctttgatata gattttaaag aaacgatgta
   103141 gtcctgtgat ggaacgcggg atgatttctg aataatgatc ttgcagatct agttgcagac
   103201 gaagtagtcc gatcagttct ttttctgtat gttccctcgg ttctttttca aatgcataag
   103261 gattcttgaa aatcccccgg ccaatcatga tgccatctac gccatatttt tcggccaatt
   103321 cttctcctgt ctggcgatct gggatatctc cgttgatcgt gatcagtgtt tgcggagcga
   103381 tttggtcacg taaagcaatg attttaggga tgacttccca atgtgcatct actttactca
   103441 tctctttacg cgtacgcaaa tggacagaaa gattggctat atcttgatga agtaggtgac
   103501 tgatccattc ttccatctca gacatttccg tatagccgat acgcgtcttg acgctgacag
   103561 gcaaaccgcc agctttagcc gcttcaatca attgtgccgc tacttctggg cgaaggatca
   103621 agccactgcc ttttccacgt tctgctacat tagggactgg gcagcccatg ttgatatcga
   103681 ttccttggaa gcccatttct gccatagcaa tgctcatttc tctaaaaaat tctggcttgt
   103741 ctccccaaat atgggcaacc atcggctgtt catcttctgt aaaggtcaat cttcctcgta
   103801 cactgtcttt tccatctgga tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga
   103861 catccggtgc tgctgcatgc tttacgacat ggcgaaatac aacatctgtt acatcttcca
   103921 ttggtgctaa aacaaaaaaa ggctttggca gctctttcca aaaattagta gtcatttatt
   103981 tgttactctc cattctatca agcagtgaag tcgcaatctt tctcaaaaac attgtcatta
   104041 tactaggaaa caaaaaaaga agcaaagtga acagcttaaa agatgtttct gattgagcaa
   104101 aaaaagactg tgacaaaaat cttatcacaa ttccttttcc ggaataaatg gtgttcaaaa
   104161 gcttgcatct gcggtaataa gtccaaccaa acgaaaaata ggaggagcta tttttgtttg
   104221 gctgttctta ttacttgatg cagaacagct ttttcacaac ctctccacta aacggtgttc
   104281 aatcagctga ccctcggtat tagctcaaca aacgacaaaa tatgaggaac tattttcccg
   104341 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttc taacttctca
   104401 atcctcttcc tttattgatc aaataccaag taacggcgta caaaacaacg atgaatagca
   104461 caagtacgat catcgatgtt gctactggca catcgattgt tccaaggaat ccgtaacgga
   104521 aaccggagat catataaacg atcggattga ctttcgaaac agcttgccaa aatggcggca
   104581 acatagagat ggcatagaaa acgccaccta gataagttaa cggctgcaag acaaatgtag
   104641 gcacgatcga tacatcgtca taagactgag caaaaatccc gttgagcagt cctgctaacg
   104701 aaaacaagat tgccgtcatc aataatgtaa tgatgaccat gaaccatgaa tacacatgaa
   104761 gtggtacaaa gaacaaggaa atgatcgtta cgagagagcc gacgagaata ctgcgaccca
   104821 ctccgccaat cacaaagccc caaatgatca catgcgtagg aacaggcgct accaagattt
   104881 cttcgatgtt tttctggaat ttttgagaga agaaggacga agaaacgttg gcatacgaac
   104941 tagtgatagc agacatcata atcaaaccag gcacgatgaa ttccatatat gaaaaaccac
   105001 ccatgtcacc gatccgacca ccgatcattt ttccgaaaat aacaaagtac aaggatgtcg
   105061 tgatcacagg cggaaccagt gtttggaccc agatacgcaa atagcggttc gtttccttga
   105121 tagctaaact ttttaatgca gtaaaataaa gattaaacat ttttctcctc cgtatgatta
   105181 ttttcttctg tgattttcaa aaacagttct tctaaacgat tcgacttatt cctcatagaa
   105241 agaactttga ttccttgtct tgtcagctga tcgaagattc cgttcacccc ttgatttcgt
   105301 tcaacttcga cagcgattgt ctgttcatct tcaaatgtac aagcgtatcc ttcaatcaca
   105361 ggtttggtct catatggcgc taaatcaaag ataaatgttt caaattgcag tttggataga
   105421 agctctttca tactagtatt ctcgatcagt tcacctgatt ggatgatccc gatgttgcga
   105481 caaagcattt cagcttcttc taaataatgc gtcgtcaaaa tgatcgttgt accattttcg
   105541 ttcaattctc ggagaaactc ccacatctcg cgtctcaatt caatatcgac tcctgctgtt
   105601 ggttcatcca agatcagcaa acgtggttca tgcatcaatg ccctagcaat catcagtctt
   105661 cgcttcattc ccccggaaag catacgtgca cggacatttc tcttttccca aagattggac
   105721 tgcttcaaat atttttcgct gcgtttcatc gcttctttgc gagagacacc gtagtagcct
   105781 gcttgattca cgacgatttg ctggaccgtt tcaaagggat tgaaattgaa ctcttgcggg
   105841 actaatccaa tctgttgctt tgctcggact agatccgtat ccagatcata accgaagaca
   105901 ttcacttttc cagatgtttt attgactaaa gaagtaatga ttccgatcgt tgtagacttc
   105961 ccagctccat ttggtcctaa tagcgcatag aaatctcctt ctttcacgga aagatcgatt
   106021 cctcgcaatg cttcgacccc acttccataa acctttttca aattatttat ttcaagcgca
   106081 tttgccattt atttcctatc tccttataga catgattctt tattgtatca tggaatacag
   106141 cagatgaaaa aacaacttgg ttccagcgaa aaatcaggtt gctgggctca ggaaaatctc
   106201 ctaatgaaag acatcaatcg gacgaagtca gacaaccttt tcagaaaaaa caaatcgaag
   106261 ataaaggatt actattcata tgtgcaacaa aaggagcgtg agacaaaagt aaagaacact
   106321 tttgtctcac gctctaaaca cgtataaacg gcggaagcaa aagccaagat ttttgatcca
   106381 ataaatactt tcgatcactt cgcctatccc aatacatgcc actgccgctt ttcatcaaat
   106441 aacgcggctt ccgtttttgc ggtttctact aaagtctctg ggtaatccaa aactttcagc
   106501 aaggcaatcg catttcgggt ccttgaaggt ccttctttta atttgaaatc aaaactgatt
   106561 ccttttcctt ctgttacttg ttcttcaaag tggacattct cacaataatt ctttaaaatc
   106621 tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag atggatactc cgcaagccac
   106681 cgaacgactg aagcggaaga agcaatccgt tcgatcgtat ttgtcccttt taaaatctca
   106741 tctacgaagc agtagcagcg ttctttggaa gccacttggt ccaacaaacg tttgatcgat
   106801 ttgatttctg aaacaaaata actgtcgcct tcaaataaat catcttcgat tgccatggaa
   106861 gtcaacacat gtccaggttg cagggtaaac tgttctgcaa tcgctgtttg gatcgtctgt
   106921 gctagaatac agctgatggc tatacttttg acatacgtag attttcctga tgcattggaa
   106981 cccgtgacta atgtattctt ctgccagttg accggattca ctaccgcttc ttttaacaat
   107041 ggatgataga tatctttcgc tgtcacaccg ccctcagtaa actctggtcg gcaagtgatg
   107101 ggcatataag tacggtaatt caatattgca gcggctactt ctaattctcc cagcaattcc
   107161 cacagcttga tggcttcagt tgaatgcttt tctaatttag tgagaacgaa atgataggag
   107221 ataaatggca gcatgaacat gatgttgaca tattcaaaaa tcaactctgc ttcactccca
   107281 ctcttcatac gaaatgaaaa acctaattta gggatgtctc ttaatggttt agtttgctgt
   107341 ttcagctctt cttgaagtgg tgtcggcagt tttgctagct gattggcggc tgaaatcgtt
   107401 gaaaccaaat aactcatgct attcagctct gtttctagtg tttgtttctt cactagataa
   107461 tagaccgtat tgaatactaa cccaccgatt gccaaataga ttcctagtac ttgtcctaaa
   107521 agaagcaaac ccaaacccac cagcggaagc aagccaagaa aaataaataa acctacgcgt
   107581 ccaatctttt tactttttcc atccgctaga tattgttttg caaaattatg atcttttttt
   107641 cctaaacgag cgaattgata ttggattttt tctctcagtt gaggattttc ctgataaaaa
   107701 gcaatcagtt tttctaactg ctgatcttca ccaaaatcaa agctgcgtaa acgttgatac
   107761 aacgcttctg aacctacact agaataggta ctgttgattc cttcgaatac ttcgaacata
   107821 tccaggtcat accaagtaag atcatcgatt tcgctatccc atgaacggaa cttcttctcc
   107881 gtgagccatg cttccttcaa gctttcttct ttatcaaatc tcggttgata aggaattttt
   107941 tcccatttgc tacgaactgt ttcctttaat ttcaaacgtg aaaaaatgtc taaagcaact
   108001 aataaaataa cagctgcaat aataattaaa acgataaatt ctctttgacc catatccctc
   108061 tccgttcctt tatttgttag ctttagtata gcactgttgc gctatttgaa aagagggtaa
   108121 aaaaacacct ttcaaaataa aaggtgtttt tttcttgtat attacatatt tatctctcat
   108181 ttatcaaaat tgacaaataa tcatagggag gaattgttgt taattcttgt ttattctcca
   108241 gtgcattgac aactgtccca agggaaagga tagcctcagg tactattttt tcttgttctg
   108301 ttggattagc taagcgaaga acaaatcctt gttcatgaaa ataagaagga taaatcgatg
   108361 atacaattaa gtcttcaggt aattcaatca atgaaaattt acgaggaaca tcaagttcat
   108421 tttgtaatgg ccaaatcttg ttatctaacc tgtgaataaa tacattcagt ttctgctttt
   108481 gataagaaat agattgttct aaccgttttt ctgcctgttt tgccactttg aactcattaa
   108541 atcttccttc ttctacatta aatccaaaag tgactttata ctcacctatc tcttgagcta
   108601 gcggtgtttc catcatgaca tgcccttcat ttgtcgtatc tcctgaagct cgcccaggtc
   108661 gccaagcaag attaggtttt ccaagttccc ctgttgttga aaataaggtt atgattaatt
   108721 gattttctat acgttcatac tcttttaaac catctgcata aaacgtaaaa taatgatcat
   108781 tttctgccac agagacactc ttatcgaata tttctaaatt aacaggtttc tctaccatag
   108841 tttctgacca attcggagga atgacatcag gggtattttc aataaatccg ttttgaattt
   108901 gtgcaattga tttttcagat ttatctaaaa cattaattgc taagcgcata cgatgcgagt
   108961 aaacagtatt atgaattttg agtgtgcctt caacgatttg actttctccc agactgaacg
   109021 atatttcata agatatttct tccatttcgg gtttttcaga taaacgatct tttaaatctt
   109081 taggaagttg agcttttccg taaacaacta atgtttcttg taaagaatct ttatatactt
   109141 tagctgtttc taattttaga gatagttctg tatctccttc taatggtgaa taatcatacg
   109201 tatcgccatc atttgctgaa tcgatcaaat gaacgaaatc aagatattgt ctatctccaa
   109261 ctatcaaatt caattgccca tctttaaaag aaagtttata tttattgtta cttatctcag
   109321 ttttgtctat ctcgattgct gtttctaatt tttcattatt tttctcaaat tcaacaacag
   109381 tgtatcctag tgctggcaca tttacattta tttttatgtc aagttcataa taagctggtt
   109441 catcaaaata ttcgaaacct gtagcaatca tccgataagc atttttccgt tctggaaaat
   109501 aaacttcttt ttcaatgata ttatagggac tacctttgaa gtgtattttc tttgaatcag
   109561 ctactacatg cacgattttc gatccactat aattctcagt cgttgtgttg aaaacaagga
   109621 cttcattcga ttttagcttt aatgcatcag ccatccgttt tacaataagg ttttcgattc
   109681 cgtcagctat ctcattagct tctttgattc gatggaaaat atcttccgca acattatctg
   109741 aaacacagcc accaatacta tcatgagcct ggttttgcaa aatctttttc cataaccgac
   109801 gaagtaggcc tttactgata tggataccat ttctttctgc aatcagcatg agcggctcaa
   109861 ctcgttttag aattttttgt tccaatttaa aattttctat tttcattttt gttcttactg
   109921 agccaataga acgatgaaca cgagagtaaa ccggctgacg aagttcacct ttatattcag
   109981 gtaattcttt tttgtttctg ataacttcta cataagaagg ataatctgaa ataatattgt
   110041 ggaatttact gtatttattc aacttctcta cgacttgatc aaaatttaaa atcattgact
   110101 tttgatctac acccgaaggt actaaaatat ctttgttttt tccttgcata tgaagcattt
   110161 caactgcttc atccaacttt tcttttgcaa attcaggaat atctttaatt gcgtctaagg
   110221 tcatcattcc agtcatataa ccaaatggaa aattcatggc ataaacaaat ttatctccca
   110281 atcctttcca gataaaataa ggagatggaa ccatcgtttc aaagtttaat cctcgccaac
   110341 acatgaaatt atcgatttct acttgatgca aaagtgtagg caaattcgca ttgaacccaa
   110401 acgtgtcagg caaataccct accatcattg gatctccata tttaaggatc gtatcactga
   110461 ttccaatcat taagtttctt aaaattgatt cagcatccac taataaggca tctgtttgtg
   110521 cataccacgg tcccacgaat agttgtcctg ttttcatcag tcgttggata accttaagct
   110581 tttgcggatt gatctccaaa tactcatcga cgattgaagt ttgtccatct aaacaaaagt
   110641 ttactttagg gttcctttcc aattcatcta gaacttcagt aaagacctga tcagacaata
   110701 cgatggcatc ttcatttgta aaataccatt ctctgtccca gtgcgtgtgt tgaattatat
   110761 gtgcatttac catatcagat tctcctactt tccttcttct actgaattta cttgttgttc
   110821 ttttttatac ttgatggatt ttagtgcatt gaccattaac gctgtaacca ctcccccaat
   110881 aacaattgat actaaatata aaggtatatt cgtaacaaaa ggaattgcca taaaggttga
   110941 aagcattgtt tcttgagtaa caccaaaaaa tccatttaga ccacatgcta ctgcagtgcc
   111001 caccacgatg cttggtatta ctcgtaaagg atctttcacg gcaaacggga tcgtcccttc
   111061 agtaatcccc acaaggccca taatcatagc tgtctttgca ttatctcgtt ccgattcatc
   111121 ataaagtttt ttgtttaaaa ttgttgctaa aaacattcca aatggcggaa tagcaattcc
   111181 taataacatg gctgtactag gtccaaagct tccgcttgaa aaagaagcaa ccccaaaagc
   111241 atatgcgatt ttattgatag gtcctcccat atcaaacgcc atcattcctc ctaaaatagc
   111301 cgacatcaaa gcagcatttg ctcctgacat gccttctagc caattagtca gtcctgttgt
   111361 cattgcttga ataggcgtag ctagtacata ccataatatg attccaacaa ctaatgtagt
   111421 aacaaaagga atcagcataa ttgattttag actaataact tgcatcggta cttttacttt
   111481 tttcaatgca ttagctaaat agccacaagc taatccagca accattcctc caataaatcc
   111541 cataccaaga ttttgacaca tcatcccagt gataaaagct ggtgctagac cgggacggtc
   111601 agcaattgaa tatgcgatat aggctgaaat catcggtacg ataaactgta atcccgtctg
   111661 accaatcatt cgcaggactc cccagaatgt atccgtctgg tcccaaacac ctgtgccacc
   111721 aaaaatgact gcaatagaag ttagtaaccc tgctgctacg acaagcggga tcatatagga
   111781 aaccccactt tggaaatgat ttagaatttc tcttccttgt ttcttgaatt ccattagtaa
   111841 ctcccccacg atatattttt tatttgttaa ttaatgcaat tgcttcatca atcgtctctt
   111901 caccatcttt tactacctct gaggtcccta ctgttaaaat cggtttaccg tcaaagcgac
   111961 ctcgttctga aacatttgta tctactgcaa atatgacaac atctgcctcg gaaacatccg
   112021 aaattgaaag tttgttttct atccccatag ccccctgtgt ttctacttta atatctaacc
   112081 ctttcttctt cgcaaacttt tctaaattag cttttgccat ataagtatgg gcaatccctg
   112141 cgatacatgc tgttactgct actatttttg tcatatgaaa ttcctccttt taaaatgata
   112201 gagcttttac caattctttt ttatttctcg aatcttttat tcttttaaca aactcgtcat
   112261 ccatcaatct tccagataat tctgctaaaa tacgtaaatg attagtacct ttatcagtgt
   112321 cttttattgc taacaaaaag acgagatcaa ctggttgatc atctaatgcg ccccattcaa
   112381 cagaccgctt taacttggct acagccactg ttgattcaat tactgctagg cttttgccat
   112441 gaggaatggc gatatttttc ccaattcctg tggtcatatg gttttctctg tcaaatacgt
   112501 ctttttgaaa ttgtttgtgg tcagacacaa acccattagt ctctaacaat ttcgataatt
   112561 tttcgactac acttttttta tcagatgcat ttaaatctat cactatatta tttatattga
   112621 ttttttcact gatcatttat aagccttctt tcaatatata aactaatttt tcaaacgacg
   112681 aactcgtttt taaatttgct aataactcat gattcgaagc gatttcattg aatgtttcat
   112741 aaattttatt caattcaggt tttacttttt cattaagtgc aaaaaagaaa acagaatgaa
   112801 caagtgtccc atcccaatca attccttctg gattaacgta gacacagatt actggctttt
   112861 ttataaaatc tacttcgata tgcggcatag caaaatcatt catcgctgtt gaagatagtt
   112921 tttctctttt gattgctccc tctagtatcc cttctttcgc gtaattttct tttaccaatt
   112981 ttttggtaat tatatttaaa acgttcactt gattatcctt tatttggtgg gtaaaaaagc
   113041 aaaatcgttt atctaataaa tttagaaaag catcttctgt ttgttcattt aactgtagat
   113101 actgttttac ttttctgaca tctgccccta acatcaaagg actaacaaca ataacaggaa
   113161 aatttgtatt ttcaatcgga atcgtgctta taacaagttc atcagtgact tttctatctt
   113221 gtagttcatt gatgcttagt acatcttcta tctcaaccat atctgcaaaa ccgttattta
   113281 ttctttgctc taataatttt gatgttccat atccgctact acaaactaat atcactttcg
   113341 ttttgtcagg tttgtaacga tccaaaagtg attgaatatg gagtgttacg taagcgattt
   113401 catcttcatt aaaatgtata cagaatctct cttcgatttc ctctaataaa tctacactta
   113461 tcataaatga ccttttaaaa gaatatttga ttttgtccgt ataagggtta tagatattta
   113521 ctcccaattt gagtctttta actgctgcat tgagatgtaa tactagatta ttgatcaatt
   113581 cttcatcagg ataaaattca tttaatttaa ttcttactaa ctcttgtagc tcaacaccca
   113641 tatctaaata ggaaagcttc gtttgatcta agtcgtgatt catagcattt ttttcaatcg
   113701 aagtaatgtg aatgtcaaca taagcttgtt catctattgg cacaataatt gaaaatgctt
   113761 tttcaatcat tttgattaaa ataagtgtat tctttgagat ttcttcttca gaattattac
   113821 tacgtgataa gaaattgctt gtttcaatcc tttctatcgc aatagcaagg tggatagcta
   113881 aagtttgaaa ttcataatct gtaaatttca attgtgtctt attcatgtat cgattaagca
   113941 attcggctac ttttttcaat atgtccttat ttaagaaatt attcgcttca ccaatgaaat
   114001 tgatattcaa gtctaagaga tctgttttac tgttagatgc tgtaaagcca ccccaataat
   114061 agttcacaac ttctgacatc aaccttcttt tacttttttc atcagctttg atgcgcacgc
   114121 catttcgatc tgaaactaag gacatcccat gcttgtttag tctttttcta acatctttca
   114181 ttgtgctttc taccgtcgac ctgctcacgt ataattcgtc agcaagattt tgaatcttta
   114241 gagaatcatt agtattcaat aatttagcaa acacataagt tagacggtcc tccggagagt
   114301 ctgcgatatt gattttatga ctggataata aatttcgaag tgtttctttt tttccaataa
   114361 gcgaaacgcc ttttttaggt tgaaccaaca tttgtacatc tggcattttt tggattattt
   114421 ctttcaaaat taaaatatct ttgctaattg ttttcgtaga tacatataat tgttctgata
   114481 attctttgta agttgtgtgc tcattttcaa tcaaaaagta tagtattttc atttgccgtt
   114541 cgctaaattc gatgcccatt atcttcacct cctgaataca gcttaagtgt acaattatta
   114601 tctctatttt catacagaag aaagttcttg attaatgtag aagtttctaa tattcttgat
   114661 tatttcaaat caataaaaaa gcctgcaata tttatatcac aggctttttt ctctattaaa
   114721 tatatataag ataatacatt tttctactag ttctcactaa tcttgtataa ttaaaccctc
   114781 cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat ctctcctccc
   114841 attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc cgtatttgtg
   114901 cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt ttgcgttcct
   114961 ggtttgatag gattggccac tgttaatcga atgaatggat cctcttttga aaatgtcacc
   115021 tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag cactcgcagc
   115081 aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa cgctacctcg
   115141 atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac ctctttagaa
   115201 aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc aagatcataa
   115261 aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt taaatacttc
   115321 tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt aatagcagtc
   115381 aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt ttcttccatt
   115441 tcttgaaatg cttgtccttt ttgttcattt tcatccaacc atgtattcag tgcatctact
   115501 aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata ttcttgtcgt
   115561 tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag gaagaaaata
   115621 aaacttccca taaagataac aacgagaagc aacagccatt taatcaaatc taaaccccac
   115681 tccccacacc gtatgaatat acacagcatg tgcctttgct agcttccctc gaagattgga
   115741 aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa ctgtttcata
   115801 aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca gcagatcgaa
   115861 ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg actctttgtc
   115921 cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga cagaagtctg
   115981 atgacgaagt tggatgccga ttcgtaacaa aagttcttcg atatcaaatg gtttcgtcac
   116041 atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc tttttgctga
   116101 aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg cttcacctga
   116161 aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc ttaacaataa
   116221 ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa gaacctcctc
   116281 cagtaaggaa tgaacagctt catcgtcttc gataatcaaa atagtttcca gcaggaccac
   116341 ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata ccacgattgt
   116401 tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt gcgcaaagtc
   116461 cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc ctttgatttt
   116521 tgcgaaaaca ccgggaatga aggccgttcc catgatataa agaaaagaaa gaagcaaact
   116581 gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg tccctaatgt
   116641 cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt tgattccctc
   116701 aacttcacca aatccgaaag caagtgtgta agcaagcatg gctagtcccg tgatcaaaac
   116761 cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat aagtcattcg
   116821 attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt ccccaatata
   116881 aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt ctaccattaa
   116941 aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat tgccgtattt
   117001 tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca tcagtcctag
   117061 tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta atagattaaa
   117121 catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc ccaactaggt
   117181 gaataccatc cacaggaact tttgcttcta ccaatgcttg attgatggct gctacttggt
   117241 caattccttc aaaaagctgg ataacacggc tagattgtac aaaataatcc ctataaccaa
   117301 gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt ataaattgac
   117361 gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct tcatgcaaaa
   117421 taccatagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa agcaagacgg
   117481 tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc atccctgctg
   117541 gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg atcagtgcat
   117601 ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc tttttccctg
   117661 tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac acaccgccgt
   117721 tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga gcaggggttt
   117781 caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca aataattcga
   117841 tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta gacttacccg
   117901 ctccattttc cccaatcaat ccatagatca ttccacgttt gatttcgatt gatactttat
   117961 ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag accgtttcat
   118021 ttccaaaatc catacttatc cttctttctt attgatgagt gaagtatagt tgacgggtat
   118081 aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa tgtcaaaaaa
   118141 aacaatcgga caatacgtgt ctttcctatt atcaggcagt ttgttatacg cttgtatcag
   118201 tttcctgatt ctttcttttc gaccaaagtc gtttctctgt attcctcttc actgaaaatt
   118261 tttgcttctt tcagatcaag cttgagtaat tttaccagac cggttataag gataactata
   118321 gcaagactcc ctcccagaaa aattagactg tctcgcagac gtggttgcgt tgtcttccaa
   118381 tcaaagagcg caccaaacag tgctaatcca agtggactgg ccaccatggt aatcgttgtt
   118441 atcgttgaca taagtcgtcc ttgtatatgc gttggaactt ctcgctgaag ccagaccata
   118501 taaaaagcaa aagaaaacat taatccttaa aaacggactt atgaaaaata atacatgccg
   118561 tttgggattt acaattagct accaaataag aggttatgac aaaagacttt tcacaatccc
   118621 ttcttccgaa taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatttatc
   118681 aaacatgaga agcaattttt gtaaattcct tcttatttct tgaagctgac cacttctgtc
   118741 acaacctcag aagcggataa ataatattgt ttgatgatac tttatctttt tcccgtttgt
   118801 ttatagatcg aatgatatta tagaccaaga atcctaccta ttaacatagc taaaaacaca
   118861 aaaaaggcac tttccaaatt gtggaaagtg ccagaaaagt taggattaag gtaactgcgc
   118921 tcagcattgc ttcgcccagt acgattctcg taactcgtga actcgaacga taatcgcaat
   118981 taacgttttg agaactgtga agctttacgg gctttcttaa gacctggttt tttcttcgta
   119041 tcattgaata agtttgaaaa accttgattt ataaaggttt tcccgtaaat atattttatt
   119101 atatttgatt aaatttgaaa tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat
   119161 atttcgtatt ttagattaaa tttttaggct cattcattct caaatccatt ctattcagtc
   119221 aataaaaatt ttaaaaagac tttataaaca aagatgttca agcgagcatc ttattttttt
   119281 gtgcttaatt tgaaaggaga acatttcatg tataaaaaac aaaaaaagaa attatgtgta
   119341 ttaactattt tgagtttggc tgttcaagca attgttggag caattgctcc tacgattact
   119401 tttgctgatg aaattaccca tccacaaacg gtaaccgtag aactagattt agcacatcaa
   119461 tatgctgttg aaggaacttt tagtgatggt cgtccaatgt cagaagtcac tgttccgcat
   119521 tatgcagtgt acaacggctt taaacaagat attttctgta ttgaaccagg agtcccgatt
   119581 tacaatgagt tcactcctgg ctatgagaaa aacccgttac cagatatgtc cgataaagcc
   119641 aaattagttt ccgttttatg gaaaaaagta ggtacagatg cagatactca tattgtggca
   119701 cagaagatga tctggcaaga agttaatggg tatacccttc actcaataaa acgatcagat
   119761 ggtagtgccg taaatatcgc agccattgag gcaaaaataa atcaagccat tgacgattat
   119821 cagaagaaac caaccttcca taatagtacg gctaaaacag tattagggca atcgactact
   119881 gtgacggata cgaataatct gaatttgtca gagttcgatg aggttgtgga aaatgccgca
   119941 aacattgatt atcgtgtgaa tggtaatcag ttgataatca ccccaaatgc caattcaaaa
   120001 gaaagtggtg tgctgacgct taagaaatct gctggtaccg gaacacctgt cgcctataaa
   120061 aaggctggcc aacagacttt aatggccggg gcgattgata aacctaatac ctacacaatc
   120121 aaaattgatg tggaaacgga gggttcttta aagatcaaaa aagtcgataa agaatcgggt
   120181 gatgttgtac caggaacggt tttccattta gactttggaa aaactttacc tgcaaaagac
   120241 gtgactactg acaaagaagg cattgctaca ttggatgaga ttcctcatgg tacaaaagta
   120301 accattaccg aaaaatcagt accggcgcct tatacgattg ataccacccc catgactacc
   120361 accattaaag caggcgaaac catttacgtg acttcaaaaa acgcacggga aaaagggcaa
   120421 atcattctgg ataaaagcgg agttgaaaca gggagtgatc tttggaatga caattattcc
   120481 ttagctggta atacgtttgc cattcgcaaa gacagtccaa ccggtgaaat cgttcaagaa
   120541 atgaccacgg atgaaaacgg tcatgcggaa acaccaaaag agattgctaa tgcgttggaa
   120601 ttgggaacct attatgtgac cgaaactaaa gctagtcatg ggttcgtgaa tacgttcaaa
   120661 cctgtaaaag tcgaattgaa gtatgccaat caaaccgtag cccctgttac cagtaacgta
   120721 aaagggcaaa atcaagaagt cactggcgaa accactttga cgaaagaaga caaagatact
   120781 ggggataaag cacaaggtaa ggcagtcttt gagggtgctg aatacacctt gtttactgct
   120841 aaggatggaa aagctgttaa atggagtgag gcttttaaac cagagttagc gaagggaacg
   120901 aaaggttctg atgaaacggt cactttgact ttagacgaaa agaaccaagt tgccgttaaa
   120961 catttagcta tcaacgagta ttattggcaa gaaacaaaag cgcccgaagg atatagcctt
   121021 gatgaaacaa agtatcctgt atccatcaaa aaggtggatg atagcgaaaa aaatgccgtg
   121081 attacccgaa atgttacggc aaaagaacaa gtgattcgct ttggctttga tttctttaaa
   121141 tttgctggat cagctgcggg cactaccgaa actggcttca atgatctgac ctttaaagtg
   121201 tcgccattgg aagggaccaa tgaaatcaca ggtgccgaag atgaagcgac cacagcttat
   121261 aacgagcaat tagggtttga tggttatggc aagtttgaaa atcttcctta tggggattat
   121321 ttacttgaag aagtagaagc cccagaaggt tttcaaaaga ttacgccatt agaaatccgt
   121381 tctactttta aggaaaacaa agaagacttt gtgaagagtg agtatgtctt taccattacc
   121441 gaacaagacc aaaaacaacc aattaaaacg gtgaccgttc cttacgagaa gctgacgaac
   121501 aaagcatttt ctgttagttt gaaccgttta atgctctatg atttgcctga ggaagaagat
   121561 agtttaactt ctcttgcgac ttggaaagac ggaaacaaag aattaacttc tctggattct
   121621 accgagctag ttgataaatt gagttacaat ctacatgaaa tcaaagaaga ctggtatgtg
   121681 gtagcgcaag ccattgatgt ggatgcgaca aaagctgccc aagaaaaaga tgaaaaagcc
   121741 aaaccagtgg tgattgcaga aacaagcgca acgttggcga acaaagagaa aacaggaact
   121801 tggaaaattc agcataaatt aaccgccgaa caagttttaa ataaaactat tgtcttattc
   121861 aactatgtgt atgaaaataa ggaagctttt gaagcaggcg ataagccagt agcgaaggat
   121921 gttagcttga ataaccaagc ccaaactgtc agttgtacag tggaacacca tgtttctatc
   121981 caaacaaagg cccacctaga aaacggttct cagaccttta ctcatggtga tgtggtggat
   122041 atgtttgatg atgtgtcgat tactcatgat gtactggatg gctcaaaaga agctttcgaa
   122101 acaattctgt atgctttatt accggatggt acaaacaaag aaatttggaa atctggcaag
   122161 attgattatg aagtaaatga taaagaattc accaaaactg tgcttgcgaa aaaagtagat
   122221 accggaaaat atccagaagg aaccaagttt acttttgcgg aaatcaatta cgataaagat
   122281 gggaatatca atggaaaaca caatgaagat ttgaaagaaa aatctcaaac cttaacacca
   122341 aaagaagttc caaccatact aagtacgccg aaacaaccgg aaacaccaac tgttccaagt
   122401 ggctctcaag aatctagtcc cacagtgaag accttccctc aaaccgggga gaaaaattcc
   122461 aatgttttac tgttcattgg ttttactttg atttttgcga cggcgggcta ttatttctgg
   122521 aatcgccgga actaaggtga tgagatgata caagtaaatc ctaagaacca acgtgcgcga
   122581 ccgcctccta aaaaagtagt cgcgctattt ttgtatccaa aactaacaat gaaaagagga
   122641 aaagaagatg gaattgaaat ttgtcgtgcc agatatggct gaaacctttg gaaagttaag
   122701 ttatgccggt gaaggagaaa ttataacaga aggatatgga cggaatacta cggtgattgg
   122761 tcgcagttat catttgtatt ccagtaagca acgggccgat gatattgagg tggtagtagc
   122821 tgcggaagcc ggtgaaaagg attttgatca agaccaaccg ctaaaagccg tgaatcccca
   122881 tttggttgcc aaaggctatg agattgagaa tcgtgggttt actgattacg tgttgtatgt
   122941 tgatgattta gtgaaagcat aggaggaaaa gaagatgaga ttaacagaag gtattgtcgt
   123001 agattcaggt ttaacatttg ggaagttgcg tttttccgca ttacgccgag aagtacggaa
   123061 gcaaaatgag gatggaacaa ttggtaatga ggtaaaagaa cgaacctata atttaaaatc
   123121 ctctgcacaa gggcgaatga ttcaagttag tatcccagct aatgtaccct tacgcgagtt
   123181 tgcttatgat gcagaggtag agttggtcaa tccaatcgtg gataccgttg ccaattatgt
   123241 ttttcgagaa ggaactaccg ttaattggtt cattaaggcg gatgatttag tcttgaagcg
   123301 gcagccgaat caaggaaatc ctacgaacca aaatgaaaga aagaaatagg tgaagattat
   123361 ggaaattaat gttgaacata ttttagattg tctcgatcaa tatggaaaag gcgagctgac
   123421 tgaggaacaa ctcgttaaag cattaacctc tgatgaaaaa atgtttctga ttatacatca
   123481 aggtttactt ggagaaaata gcgacagtga agaagactct gatgtactag actggttagg
   123541 agaagaagag tctttcttta tagttgtcga agtaaatgaa aacttatgtc ggcaggcaga
   123601 atccattttg gaagaaattg gtgtggagat gccggatgct attgaaggct ttttaaatca
   123661 attggttgaa acgaaacaac tcccctcctc gtagcagtaa atgactagca taactttttg
   123721 aaattgccta atgaaatcct attgggcttt ttcgattgga ggtggaaacc atcaaaatgt
   123781 acaaaggaca tcgcatacgc gcaggagatc agcacttggt ttatcacttt gttcttggct
   123841 ggctactcgc tctgttcatc ggttggatga gcgtctttta ttttcaagag tttagacaat
   123901 ttgatatttc taagctatca ctttctacta ttgaaatcgt ttggtctata aaagatttag
   123961 tatgtctgct tggaagtctc gctttttcag gagctatgat attgctttat attcactttt
   124021 ttcttgatca ttggcgaagc ttgtggcatc gacaaaagct agctcggatg attctagaga
   124081 accattggta tgaagtgaaa caaactcaaa gtgaaggctt tttcaaagat ttgaatagta
   124141 gccgaaccag agagactatc agttacttcc ccaaaatctg ttatcgaatg aaagatggat
   124201 tactgtctat tcgcgttcaa atttcacttg ggaaatacca agatcagctt ttaaaattgg
   124261 aaaagaagtt agaaagcgga ctgtattgtg agttagtaga aaaggaactc aaagactcct
   124321 atgtggagta tactttactg tatgatatga tcgccaatcg aattgggata gacgaagtag
   124381 tggcagaaaa tgggactctg cgattgatga aaaatcaagt atgggcttat gattctttac
   124441 cccatatgtt aatcgctggt ggcacgggtg gagggaagac ctatttcctc ctcaccatta
   124501 tcgaagcact attgaagtca gatgcagaat tgtttatcct cgatcccaaa aatgcagatt
   124561 tagctgattt aggtacggtg atgcctcatg tttattcaca aaaggaagaa atttctgctt
   124621 gtgtggaaga tttctatgaa cgtatgatgg ctcgtagcaa ggcaatgaaa gaaatgtcta
   124681 actacaaaac aggagagaat tatgcctatc ttggacttcc acccaacttt ttaatctttg
   124741 atgaatacgt ggcttatatg gagatgttaa cgacgaaaga aagtgcggtg attttgaata
   124801 agctcaaaca aatcgtgatg ttgggtcgtc aatctggttt ctttctgatt ttggcttgcc
   124861 aaaggccgga tgcgaaatat ttaggagacg gaatccgcga tcaattcaac tttcgcgtgg
   124921 ctttgggtcg tatgagtgaa ctcggatatt ccatgatgtt tggggaggtc gaaaaaaact
   124981 tctttatgaa gcacatcaaa ggtcgaggtt atgtggatac aggtggcagt gtgattagtg
   125041 agttttatac gccacttgta ccaaaagggt atgatttctt agattcaatt agtaaattag
   125101 aaaagatgaa tgattgagtg atcttcaata ctgactttat acttcaagta acgtataata
   125161 cttttatata gtaggaagaa aggggataat taatatggaa agtaatgatt cttatttaga
   125221 gtatgcaaaa gcaaataagg ataagtttat ttcttatatt atcaaaggga aatcccccga
   125281 agttggacga gacgcagttt ttatggcagg gagtcctggg gctggaaaat cagaggttgc
   125341 cattggatta gctgagaatt atgataacca tgtgattatt gatgcggatt actttcgcac
   125401 ccagtttcct gattataatg gaaaaaactc tagtctcttt caaaaagcat cttcttggtt
   125461 ggtagagcag gcatttagat atgtcttaga tcatggctat tcttttattc tggatgcaac
   125521 ttttgcaata ttaagtgctg aaaaaaatat ctcgcgggcg ttgaaaaaac attttcgagt
   125581 gacgattttc tatgtttatc aagaccctaa aatcgcttgg aaatttacac aagaacgtga
   125641 aattgcagag ggacgtcatg tgccgaaaaa ggcatttatt aacgcttttt tcaaatcaag
   125701 agagaacatt gcaaaagtaa agagtaaaca tccagatgta ttgcttcata tcataataaa
   125761 agattatacg aataatattt ccgaagttca ttatgctacg gataatctag aattattttt
   125821 accactaggg tatagtagga aagatttgga ggttcaatta catgactgag aaagaacaag
   125881 taacgaaaat tgtaaagaaa tacaataaga gcattgcgga tttatcggaa aatgctactg
   125941 caaaagagtt caaaacggtg attaaatatg tagccgatca ggccaatgaa aaacaacgga
   126001 aattagttgg attaaataaa aaatagtagg acaaattaat aatgtatgag ccaagagcaa
   126061 aatgttcttg gcttttttct ttgcaatgaa tgaggtaatg tatgagattt ttagatttat
   126121 tcgccggtat tggtggcttt cgtttaggta tggaacaggc gagccatcat tgtatcggtt
   126181 tttgtgaaat tgataaattt gctagacgta gttataaagc aattcatgat acaagtaagg
   126241 aggtggaaat gcatgacatc acaagtgtat cagatgagtt tattcaatcc ctcggaccag
   126301 tggacatcct ttgcggcgga tttccgtgcc aagctttttc aattgcggga aagcggcaaa
   126361 gattttccga tactcgaggt actttattct ttgaaatcgc tcggttcacc actcttctcc
   126421 gacctaagct tttattcctt gagaacgtcc gaggattgct caatcacgaa ggaggggcta
   126481 cgttcgagac gatcctccga acgctggatg gattggggta tgacgtggag tggtaggtgc
   126541 ttaactcaaa ggcctacatt ccccaaagcc gggagcggat attccttatc ggacattctc
   126601 gagacgcatg taccgaacaa gtatttccta tcattggacc gtcttcaaca tctgatcaaa
   126661 acatcagaaa tttggtaaat atcaatcctt ccaatcgagg aatgggtgga caagtctatg
   126721 gttcagatgg cgtagcgcca accttaacag gtgatgaagg aatcaaaatc gcactgccag
   126781 caagggatgg aatctctgta gcaggactat taccgggaaa gtttgaacaa ggtaatcgag
   126841 tttatgaagt tactggaacc gcccccacct tgtcaacgaa acaaggtggt acaaaaatca
   126901 tgattcgaaa aaatgaaaca gaatatcaag aggtgaagcc agaagacagt gtgaatctgg
   126961 cttttcctaa ttccacaagt cgccgaggtc gtctgggaaa acagagtgtt cataccttac
   127021 taacaggaga ccaacaagcg atcgtcacgg atcagtatca aattcggaag ctaacaccta
   127081 gagagtgttg gcgacttcag ggctttcccg attgggcttt tgatcgagat tctcaagtca
   127141 attcagatag tcagttgtat aagcaagccg gaaactctgt aacagttccg gtcatttttg
   127201 atattgccag aagattaaaa gagttggatg tacatggttt atgatgagtt gattggagaa
   127261 atttattggg tgattggaaa gattcagtca gacccagaat tagaagaaca attgcgacgg
   127321 ttaaaatttg atattcgcaa gaatggtgtc aaagtcgctg gcgatccata tttaatgaat
   127381 gaggagacgg atgctcgaat tgaaataaat caagtaattg cagaatttga gcggattgcg
   127441 gaccaagcaa aagaaccaga tattcgtcaa tacttgttag aaatgaaagc agagttggaa
   127501 gtaaatggta ttacagatga gtaacctaaa ccaacacaac tgactgttag gcgctgaccg
   127561 cggaggattc gcgaagcaga aatcctccgt ggtgctgtgc ctgttcatgg acggcgttag
   127621 ccgaccatga tgtctcaccc ccgtatctaa caggggggta caaaaacaca aaaaataagt
   127681 aagcaaccat taagaatgct tgttgtatca aagatagtaa gtaatttgta taggtgttaa
   127741 ctcatttgaa aaagttaaca ccttgaattt ggaggtgaga acttggcaca acggaattta
   127801 gattatcggc tattaaaaga ccgacgcaac gaatacggcg tttcacaaaa taaattggct
   127861 acggcttgtg gacttagtcg gccgtactta aatcagatag aaaatggtgg cgtaaccgca
   127921 tctataaaaa ccatgaggaa aatttttgac caactggaaa gctttaatcc cgatttgcct
   127981 ttgacgttac tgtttgacta tgtaaggatt cgctttccca caacggatgc acgaaaaatt
   128041 attcaagaaa ttctccattt gaaatttgag tatatgctcc atgaagatta tgccttttac
   128101 tcctatcaag aacagtatgt catgggtgat attgtggtga tgttgtctca tgaagaagat
   128161 aaaggtgtgc ttttagaatt aaaggggcga ggttgccgac aatttgaaac ttttttactc
   128221 gctcaaaagc gtagctggta cgactttttc gaggattgtc taaaggccgg tggcgtgatg
   128281 aaacgcttgg acttggcaat caatgatcga gtggggctat tggatatacc tgagctaacg
   128341 aaaaaatgtc agaaggaaga atgtatctcc ttgtttcgta cctttaaaag ttatcgttcc
   128401 ggggaacttt taaaggctga tgaaaaggac ggcatgggca ataccttata tatcggcagt
   128461 ctcaagagtg aggtttattt ctgtttgtat gaaaaagact atgaacaata tatcaagtta
   128521 gggattccat tagaccaaac tgagacgaaa aatcgttttg agattcgact gaaaaatgac
   128581 cgtgcttttc atgcgattca agatttgtta aaaggtcgta gcattgagag caccacgttt
   128641 tccattatca atcgttactt gcgatttgcg gataaagtag aaggtaaaag gcgaaccaac
   128701 tggcccttga atgaacaatg ggggcgtttt attggacgga atcggaaaga gattcaatta
   128761 acttccgagc caaaaccgta taccattgaa cgaaccttga attggctagg ccgacaagtc
   128821 gcccctacgt ggaaaatggc taaggagttg gatcgattga atcggacaac ctatatccag
   128881 gatatggtac agaatgcacg cttatcagac cgacataaaa agattttgga gcaacaaagt
   128941 atggcaattg aaaatttgat tatatgaaaa gaggaatcgt catgggaatt gtaaaagata
   129001 tttttttggt atttggtggc agtatgcttg gtgttactgt gatgtgcttg atgaatgcaa
   129061 gtgcagcagc ggatcgagca atggaaaaga aaggaaagtg acaagatgaa tttcggacaa
   129121 aatttatgtg cgactcgttc cttagtgaaa agctaaggca ctaataaaag aacgaaaaag
   129181 tttggttatt aggagaactg ataatctgac aggtggaatg atgaagtaac gctctgaaac
   129241 gcctgccctg agcctccgac aagcattgta tgaggaaact cagaatgttt gaagctcggt
   129301 aaagacggct gaaagatacc gtaatagttg atgaacatat atcttctatc gaaaactatc
   129361 cagaggtggg tagcgtatcc aacaggtcgg gggtctataa aatatctatg gttagaatgt
   129421 tctataagga actgacgaaa cagcgaatgt acgggtctaa atttgaactt tgtagaaatg
   129481 caaaggtacg agaaatcgta gctggtgtgg caaagtaaga atataggtta tgaaatgcca
   129541 tataacgtta caggcggtat caagccagca ggcttatagc tagtgaccta aggatatata
   129601 tgtatagata agattatcgg aacgaggaaa ggtattgggt tctcatcaag agaataaact
   129661 gacgaagaac aataagcagt tgaactaatg ctgaaaagca gagggcgaac ctaagaaatt
   129721 cttgtaatga gaatggagga atgccctcaa gtcgtgtata tattagaaaa gtattatgtt
   129781 gtttcaataa ggattacgag taagataaaa aagctcactc aatttcaagt aaaggagtga
   129841 agccttatgc ctaaaaataa aaatgaaaca ttgtgtgtag aagatttaag acacgctgaa
   129901 tattacgaaa tgcaaaacac ctttgatgat ttatatgcca aaagtaagaa tggagatatt
   129961 ttcactcatc ttatggatat tatcctatcg agagaaaata tattactggc atatcgaaat
   130021 atcaaagcaa atgcaggcag taaaacagca ggaacagacg gtacaataat taaagatatt
   130081 ggcaaattgc cagcagaaac agttgtcaaa aaagtaagat acattgttgc aggcagtcca
   130141 catgggtatc gacctaagcc agtgaggcga aaagaaatac caaaaccaaa tggtaaaacc
   130201 agacctctag gtattccttg tatgtgggac aggcttatac aacaatgtat aaaacaagta
   130261 ttagaaccaa tctgcgaagc taaatttagt gaaaacagct atggttttag acctaatagg
   130321 tcagttgaaa atgcaataaa agcaacctat aaccgcctac aaatcagcca gctacattat
   130381 gtgattgaat ttgatattaa gggtttcttt gacaacgtga accattccaa gctgataaaa
   130441 cagatatggg ctatgggaat acgggacaag cacttgatat tcattttaaa aaggatatta
   130501 aaagctccaa tcaaaatgac taacggtaca ataacatatc cagagaaagg aactccgcaa
   130561 gggggaataa tatccccact actagcaaac attgttctta atgagttaga ccattgggta
   130621 gaaagccaat ggcaggaaaa tcctgttaca aaaaactatg tggttcatat caataaaagc
   130681 ggtagcccat gtaaaagcaa cgcttacaaa gaaatgaaga aaacaaagtt aaaggaaatg
   130741 tatatggttc gttatgcaga tgattttaga gttttctgcc gctacaagga aagtgctgaa
   130801 aaagccaaaa tagcaatcac tcaatggatt gaacaacgat taaaattgga agtatcacaa
   130861 gaaaaaacaa gaattgtaaa tgttagaaaa agatactccg actttcttgg tttcaagatt
   130921 aaaatgatac cgagaagaaa gaagttagtt gttaaatcac atataagtga taaacaattc
   130981 aaaaaccaaa aagacaagct ggtatcacag gcaaagaaaa ttgcttctcc ctcaaaaggg
   131041 aaaacggaat tagaagaaac aagactttat aattcaatgg tattaggtat gcaaaattat
   131101 tatcagattg caacgaatgt aaatctggat tgtatgaaac tgaatagagc tgtaatggct
   131161 atcttcacta acagattagg tacaaacaaa aggggcagat tgagaaaaac aggcagacaa
   131221 cttacgacaa aggaggcaac aagatatggt aaatctaaaa tgcttcggta tgttgctggc
   131281 gagggcgaac ctattttccc tgttggatat atccaacaca gaaaaccaat ggcaaaggtg
   131341 tatttagcga attgctacac tctagaggga agaaaattta tccacaccaa tctaagtgtt
   131401 gataaattac tgatgtacca gttaatgaaa ttatctttgg aaaatcgcac aatagaatac
   131461 gcagacaatc gaatttcgct attttctgct caacatggaa aatgtgctat aactctggaa
   131521 gaatttcaac aagctaatga aatacattgt catcatattg taccaactgg agctggtgga
   131581 aatgacgatt ataaaaatct tattttggta aaagaggcgg tacaccgtct aatacacgct
   131641 aaaacagaag aaacaataca aaaatatata gctctattga agctaaataa agtccagctc
   131701 ataagactaa ataaatatag agtattagct ggcaatcaag aattaaactt gatataggtc
   131761 tactacataa atacttttca atataaacac gatggaacgc ggagtgcggt gaaagtcgca
   131821 cgctccgtgt gaatgggggg aaaagtttga gatatttata atatcaggaa cttacctatc
   131881 catatatcaa tggtttttaa ccaatgccca atccttagtc ttactggcaa ttgtggtcat
   131941 tggtttgttt ttaggattta agcgggagtt ttccaaactg attggctttt tggtgattgc
   132001 tttgattgcg gtcggattgg tctttaatgc ttctggggtg aaggatgtct tactcagttt
   132061 gtttaaccga attgtcggtg cgtaaggggg aagcacgatg gaacagatgc gtgtttatat
   132121 tgcgaacctc gggaaatata acgaaggtga attggtgggg gcgtggttta ccccacctgt
   132181 cgattttgat gaagtgaaag aacgaattgg gttaaatgac gaatacgagg aatatgcaat
   132241 tcatgattat gaacttccct ttgagattga tgagtatacg ccaattgagg aaattaatcg
   132301 gttgtgtggc ttagcggagg aacttgaagg aacgccagtt ggtgaagttg cttctgagat
   132361 tcaacatgct ttcttcaatt cttttgagga gatggtagag catgtggatg atattatcta
   132421 ttatccagat tgcgatgata tgagtgatgt agcatatttc ttgattgtag aagctggtga
   132481 ttttggagaa gtaccagaac acttgaagac gtatattgat tttgatgcgt gtggtagaga
   132541 cttggagatt ggaggcaatt atttggtaac gagtcgaggt gtttttgagt atccatatta
   132601 acaaaattat tgaagggtag ttgatttcta aactatcctt ctttttaaag gaatgatttg
   132661 atgaagaaaa taaaaagcta caccagtatt tgggcagttg aaaaggtcat ttatgcaatt
   132721 aatgactttc aactgccttt tcctgtgact tttaaccaga tggcttggtt tgttttatcc
   132781 ttgctgtttg tcatcgtatt tgcccatgtg cctccactat ctatgataga gggggccttt
   132841 ttaaaatact taggtatacc ggtggcagtt acttggttca tgtcgcaaaa aacgtttgac
   132901 ggtaagaaac cattgggatt tttacgatct tttattagct atcacttacg atttaaggtc
   132961 actttcgcag gaaagaaagt caaagaacag aagaaacgat gggacgaacc cattactctg
   133021 gtaaggagtg tgaactatgt acccgattaa gtatattgaa aataatcttg tctttaatca
   133081 agagggagaa tgttttgcct actatgagtt aataccttac aattactcct ttttgtcacc
   133141 tgaacagaaa tatcaagtcc acgataactt tcgtcaattg attgcgcaaa atcgtgaagg
   133201 caagattcat gccttacaaa ttgccacaga aagtagtatt cgtgccacgc aagaacgatc
   133261 aaaaaaggaa attaccgggc gattaacaga agtagcgaaa caacgaattg atttacagac
   133321 agaggcatta gtatccatga ttggcgatag tcagattgat tatcgctttt ttattggctt
   133381 caaactgatt gcgacagatg aagaggtcaa tctaaagaat ctcaagaaat ctttcttctc
   133441 cggctttcaa gaatttgtct atggagtgaa tcatcatttg atgggcgatt ttgtttcctt
   133501 atccaatgaa gaaattcgcc gatataccaa gcttgaaaaa ctaatggaaa gtaaattggc
   133561 tcgaagattt aaagtcagac gagtcacacc gagtgatctg atgtatttga ttgaacatat
   133621 ctatggcgaa aagggtacac catttgaaga atatgaattt cagctaccaa agaaaaagct
   133681 gaagtcggaa actttggtga aacgttacga cttattacgt cccagtcgtt gtttgattga
   133741 agaaaagccc cggtacttat gcatggaaca tgagaatcat gaatcatatg tagcgtactt
   133801 aacaattaat acaattgtgg gggaaatgga gtttccctcc tcagaacttt tctattacca
   133861 gcaacaacaa tttacattcc caattgacac tagtatgaat gtggagattg tgacgaacaa
   133921 aaaggcactt gctaccgttc gcaataagaa aaaggaatta aaagacttgg acaaccatgc
   133981 ctatcaatct gacaatgaaa caaattccaa tgtcttagat gcattggatt ccgtagatga
   134041 attggaaact accttggatc agtccaaaga atccatgtac aaactgagct atgtggttcg
   134101 agtaagtgcc gaatcagtag atgaactaaa gcgccggtgt gatgaagtgc tagattttta
   134161 tgatgatacc aatgtaaaac tagtccggcc ttttggcgat atgatggggc tacatgaaga
   134221 attcttacct tctagtaagc gatacatgaa tgattatatc cagtatgtta cgtctgattt
   134281 tctcgccgga cttggttttg gcgccactca gatgttaggg gagttggaag gcatttactt
   134341 tggttataac gtagatacgg ggcgcaatgt ttacctgaaa ccggcattgg cctcacaagg
   134401 ggtcaaggat tcagttacca atgcgctagc agcggcattc ctcggttcac tcggtggcgg
   134461 aaagtccttt agtaacaatc tcttagttta ttacgccgtc ctttttggtg gacaagctgt
   134521 gattgtcgat ccaaaagggg aacggggtgg ctggaaagaa accttgccag aaattgcgga
   134581 tgaaatcaat attctcaact taactagtaa gccaaaaaat caaggattac ttgatcctta
   134641 tgtgattatg gagaaaaaga aggattctga aagtctagca attgatattc tgaccttttt
   134701 aacaggaatt tctagtcgag atggggagaa gtttccagtg ttgcgtaagg ccattcggac
   134761 ggttacccaa agcaatcaac ggggattatt acaagtaatt aatgagcttc gaaaagaagg
   134821 ctcaccagtt gcggaaaata tcgcggatca catcgagtcc tttaccgatt atgattttgc
   134881 ccatttgctt ttctcagatg gttcgattac ccagtcgatt agtttagata ggcagttaaa
   134941 tatcattcaa gtagcggatt tggttttacc agatgcagaa accacttttg aagaatatac
   135001 caccatggaa ttgcttagtg tttccatgct aatcgtcatt tccacttttg ccttggactt
   135061 tatccatagt aatcgagaaa tcttcaaaat tgtagactta gatgaggcgt ggagttttct
   135121 tcaagtagcg caagggaaaa cattatcgaa taagctcgtt cgtgctgggc gggctatgaa
   135181 tgccggggtt tactttgtca cgcaaaatag tgatgattta ctggatgaac gcttgaagaa
   135241 taatatcggg ttgaagtttg cgtttcgcag taccgatatt catgaaatca agaaaacctt
   135301 ggaattcttt ggcttggatc gagaagatga aagcaatcaa aaacgcctac gagatttgga
   135361 aaatggccaa tgcttgatgc aggatttgta tggtcgggta ggcgtggttc aggtacatcc
   135421 agtctttgaa gaactcttcc atgcctttga tacccgtcca cctgttcaag agagaatgga
   135481 gtgaggggat gaaaaagaaa ctttttcgga ttgtgggaat cgtttttatt agcatactca
   135541 ctctgatagt tctattgagt ttagttggta cggtagccga ggcgacaggg ctagttgatg
   135601 atacagttga agcaggaaat ctatattcca aatattcact gaacaactat caactagatt
   135661 tctttgtgga tagctcttgg gattggttgc cttggaattg gggtgatggc ttagggaaaa
   135721 gcgtgatgta tggtctttat gccattacta atttcatttg gacggtgagt ttgtatttgt
   135781 cgaacgccac gggttatgtg gttcaagagg cttacaaatt agatttcatt tcggatacgg
   135841 ctgaaagtat tggaaagaat atccaaacat tggctggtat tactgaaaat ggactccaga
   135901 cctccggatt ttattttggg tttctattat tgatgatttt agctctgggg gtgtatgtgg
   135961 cgtataccgg attattaaaa cgagaaacca cgaaagcagt tcgagccgtg attaattttg
   136021 tgatgatttt tctactatcg ggttcgttca ttgcttatgc gcctacctat atcacaaaaa
   136081 tcaatgactt tagctcagat gttagtgaag cggcccttac ccttggaacc gagatcgttg
   136141 tacccaattc agaaagccaa ggaaaagata gtgtggattt gattcgggat agtttgtttt
   136201 cgattcaagt ccaacaacct tggttactac tgcaatttga tgattccaac atagaagaaa
   136261 ttggtgaaga ccgggtcaac aagattctat ctgtgagtcc agatgaaaac aaaggcaaag
   136321 atcgggaaga agcggtcaag gcagagattg aagacaatga caatgcgaat ctcagtatta
   136381 ccaagaccat gaaccgttta gggaccgtgg tctttttggt attgttcaat attggcattt
   136441 ccttctttgt ttttcttcta acagggatta tgttgttctc gcaaattctc ttcattatct
   136501 ttgcgatgtt tctgccaatt agtttcttat tgtccatgtt gccgacttat gaaagtttag
   136561 gaaagaaggc gattattcgt ttattcaata cgattatgat gcgagcgggt gttactttgg
   136621 tgattacgac tgcatttagc atctccacga tgtttttcaa tatttcggca acctatccat
   136681 ttttcatggt tgcttttctg caaattgtga cctttgccgg tatctatttc aaattgggcg
   136741 acattatgag tatgttcaac ctccagagta atgatagcca gtcaatggga agacgcgtca
   136801 tgcgaaaacc gcaaatgtta atgaatcgaa aattgcggca actcaatcga aatgtgggac
   136861 gagctttggc ttttggtggt ggtgcagcag tcggaaataa gttggcaaag gaacaatcaa
   136921 aatccaaatt taagccatcc ggttcttctc tacgaaagaa ttcacgatta ccgaacgatc
   136981 acgaaatact atctgactta actaaggaga atccagtttc aaataataag aaacaatccc
   137041 gaatgaattt ggctgggaga aaaatcggca aagttttgga tacccaagct ttggtgaaag
   137101 ataagactaa acaagtaaag gatcaggtaa ggaatacgcc aacgaatttg aaatacaatc
   137161 tccataaagg aatcgaaaag tcgaaaaaag caccagaaga attcaagcgc ggactgttcc
   137221 aagaaaaagc ggatcgagtg gatttgcgag aaaaacaacg acaacgaaga aatgcgaaaa
   137281 tggacgaaaa acgcaagata ctgagtgaag caacaaatcg tcagggaaaa agaagggcga
   137341 atattccaat agataaagca gctcaacctc agaaagatat gatgctgaaa aaagaagcac
   137401 caaagcggat tgtgagagca aatctgaatc ccgaaatcaa acgaaaacta actagtcaag
   137461 aaatcatttc aaaagggaaa agccaatcag caagtaagaa aaattctttc caacaagtga
   137521 aacagaggtc cactctgcca aagcagacca atcaaaaagt acaaaaaaga atgaaccatc
   137581 gtccaaaatc aaggtcaggt gagaaaaaat gaacttgaag aaagtgggtg ttatttcatt
   137641 aacttttctg accatctctt ttatgggtat tttgatgtgt gtgggtttgt tgtttggtga
   137701 agacagtgag ggaaatggta gttctggcac ttctcctggc ggaactagtg tctcagaaga
   137761 agtattgaaa caccgtgcaa tggtggaaaa gtatgccaaa gaatcggata taacagagta
   137821 tattccaatt cttttggcga ttattcaagt ggaatctggt ggcacaatgg aagacgttat
   137881 gcaaagttct gagtcagccg gattaccacc taattcatta agtaccgagg catccatcaa
   137941 acaaggctgt ctctattttg cgtctttggt taagcgttcc aaagaactag ggtgtgatca
   138001 agatagtatt attcaggcct ataactatgg tggcgggtat ctagatcatg tcgggaaaaa
   138061 cgggaagaag catagttttg ctttagctga atctttttca aaggaaaaat caggtggtca
   138121 aaaagtcgat taccccaacc caatcgcgat taaggaaaat ggtgggtggc ggtataatta
   138181 tggcaatatg ttttactgtt tattagtgaa gcaataccta actacaactc agtttgatga
   138241 caaaatagtc caagaaattt ttgatgaggc atttaagtat gaaggaacgg cctatgtgtt
   138301 tggtggctca agtccagaaa cggggtttga ttgtagtggc ttgacccaat ggtgttatgc
   138361 caaagttgac ctgaaacttc ctcgggtggc tcaggatcaa tatgatgcca tgactcatat
   138421 tgatctaaaa gatgccaaac caggtgattt agttttcttt cattcaacct atgatgccgg
   138481 tacttatgtg acccatgtgg gcatttacgc aggggagaat cgcatgtttc atgcaggaga
   138541 tccagtgggg tggacgaatc taacggaaaa ctattggcgg cagcatctta tcggagctgg
   138601 ccgatacaaa taagagaaaa gaggaaaaag aatgaagata aaaattgaac ggaatcaaaa
   138661 ggaaaagagc ccaaaaaaga aaaaagaacg agttgtatcc gtgggtaagc atcgaaaaat
   138721 ggtgcttgcc ctttggcttt tattgtcttg tagtcttgct tttggcgttt ataaaaattt
   138781 caccgccatt gatcaacata caactcatga aaaagtggtt ataaaagaga aggtagtgaa
   138841 tacttctggc gttgaaagtt ttacagagga ttttgcgaaa gaatattttt catggaaaaa
   138901 taacaaagaa gcaattgaaa agagaatgag taatctcgaa cagtacctta cagaaaacgg
   138961 tctcgcttta atccaagata tggttcgagc agatattccc acgagttccg aagttcaatc
   139021 tgtaaaaatt ctagatgttg aaaaacactc ggaagaattt gtcgtttcat ttttagtgga
   139081 gcaacatatt acagagggaa aaaagacgca atcaatttct tctgcatatc gagtgacaat
   139141 ctttgaagat gaaaacagga atcatttagt aactagtttg ccaacaatga ttggaaagcc
   139201 agacaaggca aaatatgaag caaaacaagt agaaaacgat tcagaaattg atgccaaaac
   139261 aacggaagaa atcaccgatt ttttagaaac attttttaaa ctttatccat cagcttctga
   139321 aaaagaattg gagtattatg ttgaaaatgg tgcgatgcga ccaatcaata ttgacttaaa
   139381 gttttcggag ttagagaatc cagtttatct acaaaacgga aaaaattttc tagtgagtac
   139441 aaagataaaa taccttgata atgtatcaaa aaccactagc tactttcagt acaaattagt
   139501 attacaaaaa aatggaaact ggaaaatcgt tgatgttaaa taataatgac tcaatctaat
   139561 tttattagaa gttataagta gtgtaactta tttcaggcag aagtcgccca tcctctatag
   139621 gtgggtgatg aatgccattc ggtaactcga aaaccgacaa ttaagacata gtgccttttt
   139681 attttaatcc atgtcataat cagtctatat aggaaatggt tgatatacca tggaattaga
   139741 tacaaataat cattcagttt ttcttcttgg ttaccccctt attttagtag tgaaacattg
   139801 taaacatgtc attgatgatg taatgtcagc ttatgcaaaa actgcctttg aaagaatttc
   139861 agaatcacat cacattacct tagatgaatg aaatcgtgat aaagaatact ttcatattat
   139921 gctcaaagca caacctaaaa ccgaattaac aaaattcagc aatgcctaag ctcacgtctg
   139981 ataaaatgag actttctaag ggtcaaaaat ttcctttgaa tacgattatt gcttagataa
   140041 aagtaaaagc cttagaacta cggggatagc ttggttaata agagatatcg ttgttagtaa
   140101 agacataggc ttgcaagtat gctctattcc caagaatctc ccccttcaag caccaaggat
   140161 cgttaaattc agctaagtgg caagtaattc actagacgat aattatctcc atatttcaat
   140221 ctcagctttt ccatttttca tagcaaattc cgttattttt atagcatcta aaagattcag
   140281 cttttccact gatctttcac cacttttcag cttttttatt gtgcttaaat tgataccagt
   140341 accttttgat attgaattgg aagtaaaatc attaagggtt ttttttatta aattcatatc
   140401 cgcaattcca gcgttcataa gaatgaagat tctcctctca tagactactt tgaaaatggt
   140461 actacagtgg ctgtttatat atttcagtag gttactgtag tggtcatctt attataatta
   140521 gaaaaggaac ctaaatcaaa aatgctaaag aaaattttca tagtattaag cctctcatga
   140581 ctaaagccac ggaattcttg ataaaagagt atacttgcaa gcctaaacag cggtatctct
   140641 tattaaccaa gctatccccg ttgttcctaa ggttcttgat gttttctaag gcaatgcttg
   140701 tattcatagc ccttggtcaa aatattgata ctatcgttga cgtttcgatt atgatgtata
   140761 cggcaaatag gacaagtcca ttctcgaatt gtgagtgact tcttaccatc tgtgtgtcta
   140821 cattctgaac caatttgact agacggaaat catttatcta tcttaatgat tttttgacag
   140881 aagtatcgaa aatctttaca taatctgggt gatgtattct tatgcagatg agagcgttgg
   140941 ctccgtattc ataaccagta gcctctaaaa cgtagtccat cggtgggata cctagcttgc
   141001 ggttgccagc ggctttcgca gtcttcttgt gatcaaatca agcatctcat tgagcactag
   141061 tattttgaag ataatacttg agtttttcgg cttttagaag ttcacttcat cttccatcat
   141121 ccatggaaat cccactcacc catacgcagg cctccaaaaa ttcttcaaca gtggtttttt
   141181 cagacatttt tctgaatacc tccttgtttt ttgatacgcc agatagtcag gaaaaaggca
   141241 acaaaacaca aaacaaagct gcctaaaaag gtaatcttca ttccatataa aaagacatcc
   141301 ggccgatttg cgatatagtt agttacttgc tggcccatct tagcagacat cgcttgatat
   141361 aagaccgtcg tagaaagcga aatcccgctg accatcccca aatttcgggc aaaggaattc
   141421 atcgagcctg ccagtcccaa gttttcgata gtgaccgagc tcataaccat cgtattattg
   141481 ggggactgga acaaggcatt gccggcaccc atgatggcag tagcgacaat gtatagccac
   141541 aaaggagagt ccactgacaa aaacatatac atgacctggg ttaccgacaa taagcccaag
   141601 cctactaata ccaaaatctc cgggccaacc cggtcggtca ggtagccgct taaaggcgcc
   141661 ccgacgacca tcatcaaagg aaagaccatc atcaacagcc cagcatagct aggactcaac
   141721 ccgcgagcat tttgcagata aaaaggaatc actacattga caaagaagtt ggaagaaaaa
   141781 atcaacaagg ctgtaatcaa gctcatggta aaaatcttgt tcttaaaaat accgaaagaa
   141841 atcaatggag aggctgcttt cttttcaact tttaaaaagc tgacaaaggc aatcactgaa
   141901 attacgaaca gtacaagggg gactggatgg aaaaagccac tttcttgccc caaaaagatt
   141961 gctccaaaaa agctgataat cgtagccgca atggcagcaa agccccctcc atcgattgta
   142021 tggccgctca tggtgatatc ttttggtaag aatttttcag caatcaacat cgttacgatt
   142081 cccaccggta cattgatcca aaaaatgtac gcccaagaaa attgggacaa aatcaagccg
   142141 ccaagacctg gtccagcaat cgagcctaat gaaacaaacg cacctattgc tcccaatgcc
   142201 cgaccacgtt catgtatggg aaagacttcc gtgatgattc cggcatttgt agccatagtc
   142261 atactagcac caatggcttg taccacccgt gcgaacaata aaatacctag cgaatgattg
   142321 aacccacaca acagcgatcc caagacaaag atcagcgtac caatccgaaa tactttgatt
   142381 ttcccacggc tatcgccaat tttaccaaac agcaacaaac aagaacagac aatcatcaga
   142441 taaacggaca ctacccattc cgcttgattc attggtatgc ccatatcatc tgagatggta
   142501 ggtagtgcaa tattgacaat actgccatcc aatgtggaca tgaaggtaaa catagatata
   142561 gcaatcaaaa tccaccagcg atttttttgg acttttgcat cttcttgata actatacata
   142621 ataggatttc ctttctaatg atcgtgtagc taagaacaat ataacctatg tctactgccc
   142681 aacaacttct cgcgactcta gccataggaa gcttgagtaa tttcgatttg atatcctcat
   142741 gtaaccatgt aagttggaga tgctttattt gaacttactc tatagtaact agtgataatt
   142801 cgctttactt tcttctagca atttctaaaa gtcgacttca ttgttcttca gattaatagt
   142861 gtgagctatc tcgtgagacg taagcaaatt tccattctac tttttcccaa ttttttcata
   142921 tttatggtgt tgcacctatt tgttttccaa tttgcttttc aaatacattg ctatgatatt
   142981 caattccata ggattatact aaatatcaat agtatgaata atatattata gatgtttata
   143041 tatcactgtt aattattatc cacaataatt aatgattacc tcgatatttg tcaatcaaag
   143101 gttgaaacaa tttattttca gcatcttttc tagcttgagc tgcactctta atagaatcat
   143161 acgatcctaa atagtacctc ttttttttta aagttatata cgcaatctat ttctctttag
   143221 aacgataaac tcccttaaca ccacttgtat tatttttcgg taattttgac gttagacact
   143281 ctattactgt cccttcaaca catgtccttg tttcttgcct ttttaaacat ccacaacttt
   143341 gagtatgtcc aaaaaataag ttactataac ttatctcaca catactgcca cactcacaat
   143401 gacaaaccca ttttgttttc catgaacagt tattgtccaa aggatacttt gcagtaagta
   143461 atccgaattt tttattttca atgtgatgct tttttgattt caaacatcca cagcttttta
   143521 catcaccttt tagaagtcgc tttgccgata ctaacttttt tctcccacac agacattgac
   143581 attcccaaac aacagttcct tcacttcgca gtttagttgg cttgattgca actaacttac
   143641 caaaagtttt atttgtcaaa tttttatatt tatgcctatt tttataattt ccacatccac
   143701 aacttttggt tctacctgat agtaagtttg atcgatatac aatcgtttca ttcccacagt
   143761 cacataggca acgatatttt ttatatccat tatgagtaat ctcctcaaca acagtaaggt
   143821 gaccatatct ttccttcact gcacctaccc tcccaagaaa gaagtaccat ataatttcaa
   143881 ttaactttta ataaatcaga aaaaaatatt tccaattttt cttagtctga ttttatgact
   143941 aatttacaat acaagactga acgatacgta cacgaaatcc tcaagataga taaattgttt
   144001 tcatctagtg aaacctgctc aaaatatgag cataaagacg gcaataatac actcgatatt
   144061 cgataatgga cttgttttat ttgtcaaacc catcgtaatc gagatatcaa ttccagtctc
   144121 aatatcctga tcaaaggtct aagaatccaa ccattttctt gttactttgt cagattgatt
   144181 acctttttta gtctccgaat aataaggttt tcggatcaac taacatgctc tgagaaagac
   144241 tactatcttt gcaaatttcc aaatgaaggt gtgggccagt actattccca gtacttccta
   144301 catagccaat tatttgtcct tgcaaaacat tatctccagt ttttactagg tattgttgct
   144361 ggtgagcata taacgcggtt gatccatctt cgtgttgtat caccacgtaa tttccccaag
   144421 agtagtgaaa ctctgcttta acaaccttac cagctttaac tgcatgaatc ggctcaccct
   144481 gtgtagctgc catatcaagt cctcggtgaa attcatttcc acgatttcca aacaaactgg
   144541 ttattgaata attatgaata ggcacttggt atccagtctt actcatttgt ggacctttaa
   144601 cttctttatc atagttggtt aaatcgtagg ttttaattaa ttcatttagc ttcaaatagt
   144661 attgtgtatc tgttgcatac cgtcctgtaa ggaatttagt tgcttctcga tatgttttac
   144721 tattagcttt taatgctcct gcataaaaat ctttatttcc actaattccc ttagttaata
   144781 atttggcata atcttcaaag ctttcctcat agttttcata cactctaaat tttgattggg
   144841 tcgtataaag cgttccatta cccaaatctt cgtttgttac cataatgaca aagtttccat
   144901 tataagttcc ttttatccca aataaattgt aattcggtgc ctgagctaac tggctttgac
   144961 cactggcaga ttctaagatc gcttgtgcaa tcataacaga agcatataaa tcatatttct
   145021 gaccaatttt tctcgcagac tcaccaattt tcataataaa attttctaca gattcatttt
   145081 tgtcaaaatg aatttcttca ttaccagata gtattggttg tgattggaaa gattctgttg
   145141 attctatttg ctgtggtgtc gaaaaagttt ttgtttgtcc ttccgttttt ggcgcttcct
   145201 tttttgaaga ttctatatgg tttggttttg tcactttcga agaagttatt tcacttgtac
   145261 tacttgaagt tgttgaagat tcggtacttg aaatcgtact actttcactt gacgaagttg
   145321 ttgaagactc ggcatttgaa gtcgtactac tttcacttga tgaagttgtt gaagactcgg
   145381 catttgaagt cgtactactt tcacttgatg aagttgttga agactcggca tttgaagtcg
   145441 tactactttc acttgatgaa gttgtcaaaa gttcactact ctctgctgtt acatcggaat
   145501 tactcatttc agtcgtctcg gttctttctt ttgcaatttt gtatgttgaa ttttcgcttg
   145561 ccaacacatg gaaaggtgtt gctataagga gttgggacat taatataact gctgatgcaa
   145621 gttttaaaga ttttttcatt tgatttacct ctcttttttc tctttaatag tgttttctat
   145681 taaaaattat gataaatatt ttcaatgaat tgagtaatac agtatatttc ttgatttcat
   145741 ttagtaacga ttatgtttca atgtttaatc ctgttactgc ctttaaaata aaattatagg
   145801 gttggctaaa actaggatga aataaataat ctaacatttt caatttgtct aatgtaattt
   145861 gttcttctat agcaagagaa aacattgata atagtccaga taaatcttct tgactacaaa
   145921 gttgtacacc tactattctc tttgtatttt tttcaaagct aattcttaat tttacacgac
   145981 tattttttct caaaaaagca ggtctaatga aatcttctat ttctatataa tctatagact
   146041 cacttaataa tcgtgctgtt tttaaattca aacctgctgc gtaaaaattg taattaaata
   146101 tagaaaagcc atttgtaaat tgggaaccag aaggtgtaaa atcttgattg ttaatatgtt
   146161 gagttgcaat ataagctgtt cttagagcat tagaaataga aaaatcaaca ctacttctat
   146221 taatggacgt aaaaaaagtt gtagcacaat ccccaatagc atatatatct tttatatttg
   146281 tttgctggta ttcattagtt agatatgctc cactaacatc tctcaagagt tccttttccc
   146341 caattggact attagataga aatcctatag cttgtacgac catatccata gagtattggg
   146401 taccacttgt agtgactatt ttttcaatct tttttcttcc taaatatttt ttaggacttt
   146461 ctgatagaca aagatgtatt ccattttttg atagtatttc ttcaacttta tctgaaaaac
   146521 tacggtcgta ataagtcgac aacactctat ctgcaacatc gaaaagatat acttctttcc
   146581 ctctttttct tatggcttca gcaatttcaa ctccaatata tcctgctcca ataattccta
   146641 cttttttaat agtactatca tccaaagctt taaaaatctc ttttgcattt ggaaaattct
   146701 ttatcgaaaa aatattagct aaattattcc ccggtatatc tagcataatt tgctttgaac
   146761 ctgtagctaa tataactttg tcatattctt gagagaatct tgtaccatct ttttttacac
   146821 aactaatctt tttattttga atatcaattt tgtcaacttc tgtctctaga aataactttt
   146881 ttatctcaga atttagtgtc attctatttt gataaaaaag atcttctaaa ttacttactt
   146941 ctccttttat aaataacgat gtcccacatt ccaagtaact aatttctgat tttttatcga
   147001 ttagaataac ttcataattt gaatcgctta agtttagaaa tcgagctgcg gctagccctg
   147061 aatggtttga accaataatt actatttttt ccacttaatt acattctttt cctttctata
   147121 aaaagtatat tcatctttaa ctaacactta tttcccatca taaaaaagca aaaagaggaa
   147181 gaaaaagatt cttcctcttc tcagagaaga cttaagaaat tgattttcta ttcattctct
   147241 ttttttcgat ttttataccc taataatcct aacgttccta acagtattaa accaccgcta
   147301 atagtaaaga tattactgtc actctctccc gtcttaggaa aaacttttga ggactggctt
   147361 ttgcctctat ttttgtcttg attgctgttg ctaccttgat tgtcagtctt actaccttta
   147421 ctaccgtttt ttccactgtc ttttttgttg gaagaaacaa ctacattagt aggttcacta
   147481 gtattaccag attcatcagt tgcagttact atcacttttg tccctgcctc taatttagga
   147541 actttaactg taaaattacc atttttatca gctgtcccat ggtgttgact tccgtctggt
   147601 aatgtaactg tcacctctgc atttggttca gttttaccag taacaatttg gtcaccctct
   147661 gtaataggat caacttgagg tttattcggt gcttttttat ctactacaga aatagtaact
   147721 tctacttctt ctttagatcc atctggatat gttactacta cagtaccttt tttgtttcca
   147781 gctgtactaa catctacttt ttctttccaa gtataagtag ttccttttgg tagatcttct
   147841 ttgttcttaa ttccctcaga tgcatctggc tctttattta agtctgtagt cactttttgt
   147901 ccttcaggat tatatttatc tgcatcggaa cgagtatctg tcactgtaac gtctacttca
   147961 acttcatcag atgatccatc tggatatgtc actacaatag tacctttttt atttccagct
   148021 gtgctaacat ctactttctc tttccaagca taagtagttc cttttggtaa atcttctttg
   148081 ttcttaattc cctcagatgc atctggctct ttatttaagt ctgtagtcac tttttgtcct
   148141 tcaggattgt atttatctgc attagaacga ttgtctgtca cttctactgg aactttcacc
   148201 gtatctttcg taccgtctgg atacgttact tcaatgacac cttcataatt gcctggtgtg
   148261 ttggtatcaa ttgtaccgcc gggagtaaca tctgttactg ttgttccttc tggtaacgct
   148321 ggcaagttag taacattatc tgttaaatct actgtgcctc caatttctac tttttcgcct
   148381 tctactgttg gttcatattt atcggcgtca gaacgattgt ctgtcacttc tactggaact
   148441 ttcaccgtat ctttcgtacc gtctggatac gttacttcaa tgacaccttc gtaattacct
   148501 ggtgtgttgg tatcaattgt accgccggga gtaacatctg ttactgttgt tccttctggt
   148561 aacgctggca agttagt
//
LOCUS       DANKBF010000002        85786 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000002 DANKBF010000000
VERSION     DANKBF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 85786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            22..1080
                     /locus_tag="PWY89_000136"
     CDS             22..1080
                     /locus_tag="PWY89_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2065204.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQSMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1084..2118
                     /locus_tag="PWY89_000137"
     CDS             1084..2118
                     /locus_tag="PWY89_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL2065205.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2220..2936)
                     /locus_tag="PWY89_000138"
     CDS             complement(2220..2936)
                     /locus_tag="PWY89_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2065206.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2905..3690)
                     /locus_tag="PWY89_000139"
     CDS             complement(2905..3690)
                     /locus_tag="PWY89_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL2065207.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3681..4430)
                     /locus_tag="PWY89_000140"
     CDS             complement(3681..4430)
                     /locus_tag="PWY89_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2065208.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4728..5252
                     /locus_tag="PWY89_000141"
     CDS             4728..5252
                     /locus_tag="PWY89_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2065209.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5268..6767
                     /locus_tag="PWY89_000142"
     CDS             5268..6767
                     /locus_tag="PWY89_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL2065210.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6855..7457)
                     /locus_tag="PWY89_000143"
     CDS             complement(6855..7457)
                     /locus_tag="PWY89_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2065211.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7561..8517
                     /locus_tag="PWY89_000144"
     CDS             7561..8517
                     /locus_tag="PWY89_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL2065212.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8822..9778)
                     /locus_tag="PWY89_000145"
     CDS             complement(8822..9778)
                     /locus_tag="PWY89_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2065213.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9952..10512
                     /locus_tag="PWY89_000146"
     CDS             9952..10512
                     /locus_tag="PWY89_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065214.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10505..11329
                     /locus_tag="PWY89_000147"
     CDS             10505..11329
                     /locus_tag="PWY89_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2065215.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11339..11956
                     /locus_tag="PWY89_000148"
     CDS             11339..11956
                     /locus_tag="PWY89_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2065216.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            11974..12657
                     /locus_tag="PWY89_000149"
     CDS             11974..12657
                     /locus_tag="PWY89_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2065217.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13340..14623
                     /locus_tag="PWY89_000150"
     CDS             13340..14623
                     /locus_tag="PWY89_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2065218.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14639..15526
                     /locus_tag="PWY89_000151"
     CDS             14639..15526
                     /locus_tag="PWY89_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2065219.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15543..16379
                     /locus_tag="PWY89_000152"
     CDS             15543..16379
                     /locus_tag="PWY89_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2065220.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16382..17005
                     /locus_tag="PWY89_000153"
     CDS             16382..17005
                     /locus_tag="PWY89_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065221.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17002..18294
                     /locus_tag="PWY89_000154"
     CDS             17002..18294
                     /locus_tag="PWY89_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2065222.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18653..18750
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18866..19447
                     /locus_tag="PWY89_000155"
     CDS             18866..19447
                     /locus_tag="PWY89_000155"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2065223.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19450..20757
                     /locus_tag="PWY89_000156"
     CDS             19450..20757
                     /locus_tag="PWY89_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2065224.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21173..21661
                     /gene="purE"
                     /locus_tag="PWY89_000157"
     CDS             21173..21661
                     /gene="purE"
                     /locus_tag="PWY89_000157"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2065225.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21654..22778
                     /gene="purK"
                     /locus_tag="PWY89_000158"
     CDS             21654..22778
                     /gene="purK"
                     /locus_tag="PWY89_000158"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2065226.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22791..24086
                     /gene="purB"
                     /locus_tag="PWY89_000159"
     CDS             22791..24086
                     /gene="purB"
                     /locus_tag="PWY89_000159"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2065227.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24208..25596)
                     /gene="ltrA"
                     /locus_tag="PWY89_000160"
     CDS             complement(24208..25596)
                     /gene="ltrA"
                     /locus_tag="PWY89_000160"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2065228.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26165..26542)
                     /locus_tag="PWY89_000161"
     CDS             complement(26165..26542)
                     /locus_tag="PWY89_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065229.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26798..27526
                     /locus_tag="PWY89_000162"
     CDS             26798..27526
                     /locus_tag="PWY89_000162"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2065230.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27526..27780
                     /gene="purS"
                     /locus_tag="PWY89_000163"
     CDS             27526..27780
                     /gene="purS"
                     /locus_tag="PWY89_000163"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL2065231.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27782..28453
                     /gene="purQ"
                     /locus_tag="PWY89_000164"
     CDS             27782..28453
                     /gene="purQ"
                     /locus_tag="PWY89_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL2065232.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28454..30676
                     /gene="purL"
                     /locus_tag="PWY89_000165"
     CDS             28454..30676
                     /gene="purL"
                     /locus_tag="PWY89_000165"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL2065233.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30661..32100
                     /locus_tag="PWY89_000166"
     CDS             30661..32100
                     /locus_tag="PWY89_000166"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2065234.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32123..33175
                     /locus_tag="PWY89_000167"
     CDS             32123..33175
                     /locus_tag="PWY89_000167"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2065235.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33172..33750
                     /gene="purN"
                     /locus_tag="PWY89_000168"
     CDS             33172..33750
                     /gene="purN"
                     /locus_tag="PWY89_000168"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2065236.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33747..35282
                     /gene="purH"
                     /locus_tag="PWY89_000169"
     CDS             33747..35282
                     /gene="purH"
                     /locus_tag="PWY89_000169"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2065237.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35298..36572
                     /gene="purD"
                     /locus_tag="PWY89_000170"
     CDS             35298..36572
                     /gene="purD"
                     /locus_tag="PWY89_000170"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2065238.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36694..37608
                     /locus_tag="PWY89_000171"
     CDS             36694..37608
                     /locus_tag="PWY89_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2065239.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37741..39306)
                     /locus_tag="PWY89_000172"
     CDS             complement(37741..39306)
                     /locus_tag="PWY89_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2065240.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39299..40750)
                     /locus_tag="PWY89_000173"
     CDS             complement(39299..40750)
                     /locus_tag="PWY89_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2065241.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40764..41780)
                     /locus_tag="PWY89_000174"
     CDS             complement(40764..41780)
                     /locus_tag="PWY89_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2065242.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42000..43088
                     /locus_tag="PWY89_000175"
     CDS             42000..43088
                     /locus_tag="PWY89_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL2065243.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43142..44668
                     /locus_tag="PWY89_000176"
     CDS             43142..44668
                     /locus_tag="PWY89_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2065244.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44670..45875
                     /locus_tag="PWY89_000177"
     CDS             44670..45875
                     /locus_tag="PWY89_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2065245.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46132..47109
                     /gene="guaC"
                     /locus_tag="PWY89_000178"
     CDS             46132..47109
                     /gene="guaC"
                     /locus_tag="PWY89_000178"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL2065246.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47373..48797
                     /locus_tag="PWY89_000179"
     CDS             47373..48797
                     /locus_tag="PWY89_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2065247.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            49258..50367
                     /gene="pdhA"
                     /locus_tag="PWY89_000180"
     CDS             49258..50367
                     /gene="pdhA"
                     /locus_tag="PWY89_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL2065248.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50370..51347
                     /locus_tag="PWY89_000181"
     CDS             50370..51347
                     /locus_tag="PWY89_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL2065249.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            51372..53015
                     /locus_tag="PWY89_000182"
     CDS             51372..53015
                     /locus_tag="PWY89_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL2065250.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            53023..54429
                     /gene="lpdA"
                     /locus_tag="PWY89_000183"
     CDS             53023..54429
                     /gene="lpdA"
                     /locus_tag="PWY89_000183"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2065251.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            54591..54866
                     /locus_tag="PWY89_000184"
     CDS             54591..54866
                     /locus_tag="PWY89_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL2065252.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            54872..55639
                     /locus_tag="PWY89_000185"
     CDS             54872..55639
                     /locus_tag="PWY89_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL2065253.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            55807..57636
                     /gene="typA"
                     /locus_tag="PWY89_000186"
     CDS             55807..57636
                     /gene="typA"
                     /locus_tag="PWY89_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2065254.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            57897..59669
                     /gene="recQ"
                     /locus_tag="PWY89_000187"
     CDS             57897..59669
                     /gene="recQ"
                     /locus_tag="PWY89_000187"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL2065255.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            59783..60085
                     /locus_tag="PWY89_000188"
     CDS             59783..60085
                     /locus_tag="PWY89_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL2065256.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            60088..61251
                     /locus_tag="PWY89_000189"
     CDS             60088..61251
                     /locus_tag="PWY89_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2065257.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            61277..64705
                     /locus_tag="PWY89_000190"
     CDS             61277..64705
                     /locus_tag="PWY89_000190"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL2065258.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            64726..65850
                     /locus_tag="PWY89_000191"
     CDS             64726..65850
                     /locus_tag="PWY89_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065259.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            65840..66277
                     /locus_tag="PWY89_000192"
     CDS             65840..66277
                     /locus_tag="PWY89_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2065260.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            66261..66599
                     /locus_tag="PWY89_000193"
     CDS             66261..66599
                     /locus_tag="PWY89_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL2065261.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            66718..67278
                     /gene="rsmD"
                     /locus_tag="PWY89_000194"
     CDS             66718..67278
                     /gene="rsmD"
                     /locus_tag="PWY89_000194"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2065262.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            67275..67766
                     /gene="coaD"
                     /locus_tag="PWY89_000195"
     CDS             67275..67766
                     /gene="coaD"
                     /locus_tag="PWY89_000195"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2065263.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            67756..68799
                     /locus_tag="PWY89_000196"
     CDS             67756..68799
                     /locus_tag="PWY89_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL2065264.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            68958..69686
                     /locus_tag="PWY89_000197"
     CDS             68958..69686
                     /locus_tag="PWY89_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2065265.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            69802..70047
                     /locus_tag="PWY89_000198"
     CDS             69802..70047
                     /locus_tag="PWY89_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2065266.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            70044..70262
                     /locus_tag="PWY89_000199"
     CDS             70044..70262
                     /locus_tag="PWY89_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL2065267.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            70398..70937
                     /locus_tag="PWY89_000200"
     CDS             70398..70937
                     /locus_tag="PWY89_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL2065268.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            70930..73203
                     /locus_tag="PWY89_000201"
     CDS             70930..73203
                     /locus_tag="PWY89_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2065269.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            73241..74287
                     /gene="holA"
                     /locus_tag="PWY89_000202"
     CDS             73241..74287
                     /gene="holA"
                     /locus_tag="PWY89_000202"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2065270.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(74376..74630)
                     /locus_tag="PWY89_000203"
     CDS             complement(74376..74630)
                     /locus_tag="PWY89_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2065271.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(75030..75299)
                     /locus_tag="PWY89_000204"
     CDS             complement(75030..75299)
                     /locus_tag="PWY89_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065272.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(75769..76224)
                     /locus_tag="PWY89_000205"
     CDS             complement(75769..76224)
                     /locus_tag="PWY89_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2065273.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(76362..78290)
                     /locus_tag="PWY89_000206"
     CDS             complement(76362..78290)
                     /locus_tag="PWY89_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2065274.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(78487..79320)
                     /locus_tag="PWY89_000207"
     CDS             complement(78487..79320)
                     /locus_tag="PWY89_000207"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL2065275.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(79512..80216)
                     /locus_tag="PWY89_000208"
     CDS             complement(79512..80216)
                     /locus_tag="PWY89_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2065276.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(80239..80865)
                     /locus_tag="PWY89_000209"
     CDS             complement(80239..80865)
                     /locus_tag="PWY89_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2065277.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            81191..83566
                     /locus_tag="PWY89_000210"
     CDS             81191..83566
                     /locus_tag="PWY89_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2065278.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(83590..84081)
                     /locus_tag="PWY89_000211"
     CDS             complement(83590..84081)
                     /locus_tag="PWY89_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2065279.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            84268..85188
                     /locus_tag="PWY89_000212"
     CDS             84268..85188
                     /locus_tag="PWY89_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2065280.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            85342..85686
                     /locus_tag="PWY89_000213"
     CDS             85342..85686
                     /locus_tag="PWY89_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL2065281.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        27621 a        14999 c        18414 g        24752 t
ORIGIN      
        1 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
       61 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
      121 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
      181 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
      241 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
      301 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
      361 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
      421 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
      481 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
      541 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
      601 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
      661 cagactgcac cgtggctatt tcagtcgatg gattatttag ctggtttaag ttcaccattg
      721 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
      781 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
      841 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
      901 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
      961 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     1021 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     1081 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     1141 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     1201 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     1261 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     1321 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     1381 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     1441 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     1501 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     1561 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     1621 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     1681 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     1741 atcaatgaag gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt
     1801 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     1861 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     1921 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     1981 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     2041 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
     2101 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
     2161 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
     2221 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
     2281 cggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
     2341 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
     2401 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
     2461 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
     2521 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
     2581 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
     2641 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
     2701 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca
     2761 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
     2821 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
     2881 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
     2941 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
     3001 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
     3061 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
     3121 cccgacaccg tcatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
     3181 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
     3241 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
     3301 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
     3361 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
     3421 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
     3481 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
     3541 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
     3601 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
     3661 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
     3721 aatcgtctct ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa
     3781 tccaatagct gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata
     3841 gttttatcag aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt
     3901 tcacttaact gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt
     3961 tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca
     4021 atcatttttt gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa
     4081 actgcaccaa taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct
     4141 aacattcctt ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca
     4201 tgacttgaga aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca
     4261 gggtccatga caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct
     4321 tgaatcgcat gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca
     4381 acgattttag caatgctaga gattttatct gacacactag atcggttcat cgaaagaata
     4441 aaattcctga tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca
     4501 aggatgatcg tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac
     4561 ctcgaaagaa tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac
     4621 gataaacaca accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat
     4681 tttcaaacat tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa
     4741 tcgcacttga gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat
     4801 tgattatttt tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc
     4861 cttggttaac tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat
     4921 ctttattttt atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt
     4981 tctatatatt gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc
     5041 tggaaggaca agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc
     5101 agttgaaaag tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac
     5161 agacagctga gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg
     5221 tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag
     5281 ttgtttttat cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg
     5341 tagcgaagta ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag
     5401 gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg
     5461 gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat
     5521 tggacgtctc cacccctgaa gtgtatacgg aacaaggcgt accggtgatg tgtgatggta
     5581 cttcgatcat caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt
     5641 taggcaagac aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc
     5701 gctcgattct tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc
     5761 aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt
     5821 tcactatcaa agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa
     5881 tcgcccaagt caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta
     5941 tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga
     6001 tcgcagaatc attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg
     6061 ttgctaaagc aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag
     6121 taatcgaaca ggaaatgcag attaaaatcg tagaacgtca aaaacaaatc gaactggaag
     6181 aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg
     6241 ccgatcggta cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag
     6301 ccgaagcaga acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt
     6361 tagctggaca agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa
     6421 aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta
     6481 tcgatatgct accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa
     6541 tctctgttgt cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa
     6601 attatgccac aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg
     6661 acttgaagga aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt
     6721 atgaagcgcc aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc
     6781 accgttcaat caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt
     6841 attgcaaaag aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc
     6901 tcttgtaaat ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat
     6961 agttcttcct tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg
     7021 aatgtcccta taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga
     7081 gggatgctca acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg
     7141 ttaataccat ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct
     7201 accaaaaaat cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca
     7261 cctaatgtat tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga
     7321 cgcaaagaaa aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga
     7381 aaaaacaaga tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga
     7441 aaacggccaa attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt
     7501 ttgtctgata gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa
     7561 atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca
     7621 gcgggtagct tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta
     7681 ggaaatacca agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta
     7741 gctacggcaa cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt
     7801 ctttttttat tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta
     7861 gtaaattcgg ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc
     7921 ggcaatgaag cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac
     7981 atgccagatc gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga
     8041 aatattttga gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc
     8101 aaatgcgaac atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa
     8161 gaaaactact ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca
     8221 caggccaatg tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat
     8281 tatggaaaac atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat
     8341 gatggcagac aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct
     8401 ctgccgacta gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct
     8461 tttaaaagtt ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg
     8521 ttgtgaattc agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg
     8581 tgttctcctg tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
     8641 ttttgaaatt gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa
     8701 cggtgttcaa aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc
     8761 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa
     8821 attacatatt tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca
     8881 agaagagaga gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt
     8941 aattcattgc ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata
     9001 agatctccgc acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg
     9061 caacgatacc gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat
     9121 tttctttgtt acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca
     9181 ttgtagaggc aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat
     9241 acgtaccgat atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag
     9301 ggtctggttc tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc
     9361 ctgtatccat tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt
     9421 ctgctaagat caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat
     9481 tcatcgtgat ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat
     9541 ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta
     9601 acatttcttg atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg
     9661 ctttttctga agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt
     9721 cggcaaactc atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct
     9781 gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc
     9841 acgaagaata aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga
     9901 ggtgcttttt gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa
     9961 gtatatattg atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt
    10021 ttagatgaat acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa
    10081 aatctgccat ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg
    10141 ctaacgcaaa agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt
    10201 gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca
    10261 aaagtcgagc tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa
    10321 gtaagtattc aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca
    10381 gatgttcaaa aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat
    10441 ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt
    10501 tcaagtgaat aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta
    10561 tgttattttt agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac
    10621 aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa
    10681 ggacaaagaa acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg
    10741 gaagctagtt ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca
    10801 attggcatca ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc
    10861 atttgccaag gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc
    10921 tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg
    10981 tgtatcagaa gaggaggcga caaagattac taaagaaacg attacggagc caggatacag
    11041 cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc
    11101 ttctactgta ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa
    11161 tgctgcgcag tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat
    11221 cacatatgaa ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa
    11281 acaccaattg acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat
    11341 ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc
    11401 cggtgtagca gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt
    11461 tactgataat cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt
    11521 tccgcatgcc caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca
    11581 agctatatta gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt
    11641 cgggatcaag gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa
    11701 cgaagaatgg ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat
    11761 ggatgaccat acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa
    11821 agtgattacg gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc
    11881 gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca
    11941 gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc
    12001 atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg
    12061 atcaagatat ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata
    12121 cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt
    12181 actaaaagca ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg
    12241 acacaaggtg ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg
    12301 gtcgttttga acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat
    12361 attccagtta tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct
    12421 ggtatttcga ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt
    12481 cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt
    12541 gctgagacca ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag
    12601 ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag
    12661 gtgagaatca aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag
    12721 tactatttgt tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta
    12781 cataagaaga gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt
    12841 tattgaagcg ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat
    12901 ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc
    12961 catagtcaat gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag
    13021 ttatttttct gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc
    13081 aaggatattg ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt
    13141 gaagcaagaa gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa
    13201 gtattgagtt gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg
    13261 aaaaagatcg tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat
    13321 ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat
    13381 gttattcttc ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc
    13441 ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga
    13501 aaaaaatccc catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa
    13561 attaggtaca ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt
    13621 tcctcaatac atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga
    13681 tatttctaat gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg
    13741 actgtctaac ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt
    13801 aaatatccct gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca
    13861 aatcactgaa gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg
    13921 tattattcaa ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc
    13981 taacagcttg ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa
    14041 tctggtagac aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct
    14101 gtcttttcag gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt
    14161 gacagaaata ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg
    14221 cagaaaagca gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc
    14281 aaaaagctca gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga
    14341 agaagccaat gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga
    14401 acagctcgct cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat
    14461 ttccgatatg gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca
    14521 agaccaatac aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaagaagc
    14581 agcagagagt ctttatgagt ttgcgtcaaa cattctagga tagcagcagg aggaaggcat
    14641 gaaaaaagaa aaactaaaac ggttcttgga tcaagacaat gtggtcggtt acgtatttgc
    14701 ttctccattc atcctaggat ttctattgat aacagtgata ccgatgatct tgtcacttta
    14761 ttattcgttc accgattatc gtttgggaaa tccagtctca tggatcggct tggataatta
    14821 tataaagatg ctgagtgatg cccgtttttt gaactccatc cgagtcactt ttctctatgt
    14881 atttgcatct gtacctgtga aactgatctt tggtctattg attgcttatc ttttaacgag
    14941 gaatatcaaa ggaacgactt tctttcgttc tttgtattat gtgcctagtt tgatcggtgg
    15001 cagtatttct atcgctctag tttggcggga gttgttttct aaaaacgggt tagtgaatat
    15061 tttcctggaa cgattaggcg cacctgcgct ttcctggttt agcgatcaaa aactggtcat
    15121 gatccctctt gttttgatgt ctgcttggca attcggttcg gcaatgatca tctttgctgc
    15181 aggtatcaaa gagatcccag gctcgtatta tgaagcggca gagatcgatg gagcgaacaa
    15241 atggcagacg ttttacaaga tcacgttacc tcttctttcg ccgatcattt tgtataactt
    15301 ggttatgcag acaatcgcgg cgtttatgac ttttacgcag gcatttatca ttacccaagg
    15361 tggtccgaat gatgccacga acttttatgc tctttatgtg tataaccaag ctttttcttt
    15421 taattctatg gggtatgcta gtgctatttc gtggatcatg ctgttgatta tgggagctgt
    15481 cacctttctg attttcaaat catcaaaaaa atgggtcgtt gtgtgaatga gaggaggaag
    15541 aaatgaataa tacagtaacg aaatggtttc aatacggttt tattttgcta ttaggtctag
    15601 tcatgattta tccggtctta tggatgatcg caggttcttt taaaacggat caagagatta
    15661 tgagtggttc gctgaatttg attccagaga cttttcaatg ggaaaattac gcaaaaggat
    15721 gggcggggtt tgctggaatt tcatttttta ctttctttag gaattcgttt gtcatctctg
    15781 cagtttcgac gatagggaca gttctgagct cgacttgtat tgcttacgca ttatcgagaa
    15841 tcgatttcaa aggaaaaaga ttctggttcg tcgtcatgtt agtaacgatg atgattcctg
    15901 cacaagtgat tttgattccg cagtttatta tttacaatcg gctgaatttg gttgggacgt
    15961 atgtgccgct gattttacca catttttttg gacaagcatt tttcatttac cagatcatgc
    16021 aattcatggt cagcatcccc aaagagttag atgaatcagc gattattgac ggatgcagca
    16081 agtatagtgt ctttaccaaa attatttttc ctttgctgaa accttcgatc atcacgacga
    16141 tcatcatcca gttttattgg aaatgggacg acttcatggg accattgatt tatttaaaca
    16201 agccgagatc ttacacggta tcgattgcca tcaaattatt tgcagacgct ggatctagta
    16261 cgacttacgc gagtatgttt gcgatgtcta cgttgtcgct gctaccggtt tttctgattt
    16321 tcttattctt caataaatat cttgtccagg ggatcagtac aagcggtctg aagggctgaa
    16381 tatgaaaaag ccaatagaag agatgtttct ttacaagctc aactactatg tctgtcggat
    16441 ttttttaacc agtcttatca gtaatgctct tttcgcttta gttctaggat gtttgttcac
    16501 tttaggatta gtgctgtctg ctcgtgttgt ctatctgatt tattttttga tgatgtatcc
    16561 ttcttattat ctatctgtcc attacttggc agaaaaaaag gtatgttgga ggaaaggaaa
    16621 aatcaaacgg atgtgtttca tcattacgtt gtttcagctg tttgtcatta tttttgcagt
    16681 tgattggtat ttattagtgg gaaagattgg agcttggtat ctgattcctt ttctattgtg
    16741 tctgttgctt tttgtatccc atagtttttt tagtacgcta attgatatct gcaaagaaga
    16801 taaagcagat agtcttgttt atttgatcaa aaggaatatc tggctgttgt caaaaaacgt
    16861 caaggggttt ctgtggtatt tatgcctttt gtatggactg ttctggtgta tacagcatga
    16921 ttttttactc actgcattac tgtatccagg gctgccattt gtcacagtat tttgcttaaa
    16981 agaaaaggag aatgacttta aatgaagaaa aaatatgtac aagtcggact tggtgggaga
    17041 gcgcgttttt tttatgaggc aattgcctct acgtatcatc aaacttgtga actagtcggt
    17101 ttttgtgatg tatctcaaac aaggatggat tatgcaaatc ggatacttgt gaacaagtat
    17161 ggacagaaaa aagtaaagac atgtatgcca tcacaatttg agcaactact ggatgacgta
    17221 cggccggctg aagtcattat tacgtcagta gatcgaacgc atcatgatta tatcattcgt
    17281 gcaatgaaaa aaggatatga tgttatttgc gaaaaacctc tgactatcga tgaggaaaaa
    17341 gctcaagaaa taataacggt ccaaaaacag acgggaagaa gcctgcgagt cacttttaat
    17401 tatcgttatg caccgtatca cacgaagata aaggaactga tagaaagtga tgtgattggt
    17461 gatgtgtttt ctatccactt cgaatggctg ttaaatacgc aacacggggc agattatttc
    17521 agaagatggc atcgaaataa gcacaatagc ggcgggcttc ttgttcataa atctacccat
    17581 cattttgatc tagtgaattt ttggttgaac agccgtcccc aaaaggtatt tgcattcggt
    17641 ggattaaact tttatgggat gcataatgcg gagaaacgcg gagtagaaat attttacagt
    17701 cgatgctatg gaagcgaact agctaaagct gatccatttg ctatagacct gtcagccaat
    17761 gaggaattgc gagaaatgta tttagaagca gaaaaagaca gtggatatat tcgtgaccgc
    17821 agtgtatttt ccgatgatat ctctattgaa gatacactag gcgtaacggt caaatatgaa
    17881 actggtgcga ttttgacgta ttcgctaaac gcgtatatgc cgtgggaagg attcaatgtt
    17941 tctttcaatg gcagcaaagg acggatcgaa atgaaagtga tcgaaaagtc ttacatcaat
    18001 gcaggtggag ataaaaagga tgaaggagca gccgttcttc agtccatcca tgtctatccg
    18061 atgttcggtg agccatatga ggtaatcgtc gaacaagaag cgggcggaca tggcggagga
    18121 gatccgcgtt tattagatga tctatttggt gaagaaaaag aagaagatcc gtggaatcgg
    18181 gcagcaactc acgtagatgg tattttgtcg attttgacag gaatcgcagc aaatcattct
    18241 attgcttcag gaaaagcagt agtgattgac gagcttgtca gcttaaaaga ataagaatag
    18301 atttacggaa attacttccc gtagtttata aaatcttgcc ttattgccga taggctgtct
    18361 tttgaacact atttattcgg aaaaaggggc tatgacatgc tttttgtcat gccttctttt
    18421 tatgtgggct gcttttttag ataggagaaa ataaaaataa ctatttaaac ccgaacaaaa
    18481 aggaataatg cattaaaaat cattagattt tacaaaaaaa taagtaaacc acgttgtcag
    18541 tttcagaaag tagtgttaga ctgttttcaa tatttgaaac aacatttttt attcaaatat
    18601 ggcaaactgc cttcacgtgc aaaacagttg caagaaaatg aatttacact attattagtt
    18661 ttacatacct acttatatat cgtcataata tggatgacag tttctagcca gtaccgtaaa
    18721 tgctggacta taagtaaaag attggctatt tcacccttct tttcttatga taaagtggag
    18781 ggtgtttttc tttaacagaa acatagtagg tttttgcaca aaagcggtta tttcggtatt
    18841 aataaatgat aaaggagcaa ataaagtgaa agaattagtt gaacgtattc aaaaagacgg
    18901 acgtatatta ggcgagggcg tattaaaagt cgacagcttc gtcactcacc aagtagaccc
    18961 tgtattgatg gaacaaatgg gaaaacggtt tgcagaagta tttaaagaac aaggaatcac
    19021 gaaagttgtg accattgaag cttctgggat cgcaccagct ttatttgctg caagagaatt
    19081 agatgttcca atgatcttcg ctagaaaagc aaaaagtttg acgatggatg aagaattatt
    19141 gacagcttct gtttattctt ttacgaaaca agtgacaagt acgatttcta tttctcgtaa
    19201 atttttatct tcagaagaca aagtattgat tatcgacgat ttcttggcaa acggacaagc
    19261 ggcaaaagga ttgatcgaat tatgccagca agctggagcg caagtagaag gaatcgggat
    19321 cgtcatcgaa aagtctttcc aagatggacg tcagttactt gaagaaatgg gattgagagt
    19381 cgtttctctt gcgcgtattg cttccttgac gaatggacaa gttgagtttc ttgaggagga
    19441 cgcataatcg tggaaaaaga aacacaaaat ggaaaagctg cggttttagg gctgcagcat
    19501 ctgctagcga tgtatgcagg agcagtagcg gtgcctcttt tgatcggtac aggactaggc
    19561 tttaatcaag aacagatgac ttatttgatt tcaattgata tctttatgtg tggattagcg
    19621 acattattgc aattgacagt taaccgattt ttcggaattg gcttgcctgt cgttttagga
    19681 tgtgcgatcc aagcagtagc tccactgatc ttgatcggaa ccaatgaagg agtaggagct
    19741 atctacggat ctattatcgc ttctgggatc tttgttgtgt tagtttctgg tgtcttttct
    19801 aaaattaaaa aactattccc tcctatagta acaggaacgg ttatcaccgt tattggtttg
    19861 actttgattc ctgtagcaat cgagaaaatg ggtggaggta atgcaacagc agctgatttt
    19921 ggtgacaaaa caaatctgct acttgcattt gtgacgattc tcctaattgt aggcgtgcaa
    19981 atgctggcaa aagggttcat tcgttcaatc gcggtattga ttggtttagt cggaggaagc
    20041 atcttagctg ctttcatggg aatgattgat ctgagcgcga ttggagatgc gccgttgttc
    20101 catgttccac aaccctttta ttttggtaaa ccaactttcg acgtatggtc gattttacta
    20161 atgatcatca tttccatggt cagcatggta gaatcaacag gtgtatattt tgcacttggt
    20221 gacattactg ggaaaaatgt tggagaagaa gaattgaaaa agggatatcg tgcagaagga
    20281 cttgccgtta tcttaggcgg tattttcaat acattccctt atactggctt ttctcaaaat
    20341 gtcggattgg ttcaactttc gggaatcaaa acaagaaaac ccatctattt ctctgcattc
    20401 ttcttgattg ttttaggatt attccctaaa atcggtgcgt tagcacaaat catccctgag
    20461 ccggtacttg gtggaggaat gttagtcatg ttcgggatgg tggcagtcca aggaatccgt
    20521 atgctgttgg aagttgattt cagcaatgat aaaaatctat tgattgttgc tgtttctgtt
    20581 ggatttgggc tgggtttcaa tgttacgccg acattattca gtcaaatgcc gcaaacattg
    20641 caaatgttca ctggaaatgg catcgtaatg agtagcatta cggctatcat attgaatttg
    20701 atctttaatg gattgaaaaa agatgaagaa atattggaag aagccttcaa gaagtaatag
    20761 agcaaaagaa aggttgtgaa atcagcgttt tgacctgatt gttagagaaa caaacggaaa
    20821 aatagttcct cgtattttgc cgtttgtttg agctaatacc gagggtcagc tgattgaaca
    20881 ccgttgaagg aagaggtcgt gagcctgccg ttcatctccg agtcacaagg aacaatttca
    20941 aaaaaaagct tctcatgttt gttgaatatt gttccttgtg accgaggagt tggcaggaga
    21001 acaccgttta tccataaaaa ggaatgtgac ctaacttttg tcacattcct tttttttatc
    21061 tttaataaac acgaacaata aaatgttttt ctttgttaac attcgaaatt tctttgacat
    21121 caaaaaaagt gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa caatgacagc
    21181 aaaaatctct gtgattatgg ggagtacttc agattgggaa acaatgaaag aagcatgtca
    21241 aatattggat gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc
    21301 agatttgatg tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc
    21361 aggagcaggg ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa ctttgccggt
    21421 tatcggtgta ccggtccaat cacgaacatt aaatggtttg gattcgcttc tttcgattgt
    21481 ccaaatgcct ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa
    21541 tgctggacta cttgcgatac agatgctttc catgtatgat ttagaattgg aggctaaatt
    21601 ggctgaaaga cgttcaatgt tggctaaaac tgttatagaa agtagtgatc aacttggtta
    21661 aacctttgtt gcctggttca acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga
    21721 tggcgattag tgcaaaagaa atgggcttta aagtaggtgt gctggatcct gtgagcgatt
    21781 gtccagccgc acaagttgca gattggcaca ttgaaagtgt ctatgatgac acctttgcgc
    21841 tgaaagagct tgccagaaga acagatgtca ttacctatga attcgaaaac gtcagtgtag
    21901 aggcgttaaa tgcgatttta cccatgtcat ttattcctca agggacagac ttgcttgcga
    21961 ttacgcaaga ccgcctgtta gaaaaatctt ttttggaaac gaataacatc gtgattgctc
    22021 cttatgcaac gatcgtcagt ccgacggata tccaagatgc gattgacggg atcggctatc
    22081 cttgtgtttt gaaaaccact cgaggcggct atgatggcaa ggggcagtat gttttgaaaa
    22141 gtcgggcgga tctagctcct gccatggacc ttttaagaga aggaacctgt gttttagaag
    22201 catggatccc gtttgaaaaa gagatttcga tcatggtggc aggaaatggt caaggagatt
    22261 tcacgacttt tccggtcgtg gaaaacatcc accacaacaa tattcttcat gaaacgattg
    22321 ctccagccgc gattgatcag gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg
    22381 cagaagcagt gagcctttca ggtgttttag gggtagaaat gtttttaaca aaaacaggcg
    22441 ggctatatgt gaatgaatta gctcctcgtc ctcacaattc tggacattat tctattgaag
    22501 cttgttctat gagtcagttc gatgcccata ttcgaggtat ttgtggttgg ccattaggag
    22561 aaaaagtacg tttgataagc aatgcagtaa tggtcaatgt tttgggagaa caactgctgg
    22621 aaagctatcg cttgattgag aataagccag actggcaatt ccatttctat ggaaaaaaac
    22681 aagcaaaaaa aggtcgcaag atgggacata tcactatctt gacagaagac atccagcaaa
    22741 cgctggaaga aatcaaacaa acgaatattt gggattagag gagaaaaaac atgttagaac
    22801 gttatacacg ccctgaaatg gggaatatct ggacggaaga gaacaaatac cgtgcgtggc
    22861 tcgaagtcga gattttagca gatgaagctt gggcagaact tggtgaaatc ccgcaagaag
    22921 atgtgaaaaa aattcgtgag catgcatcat ttgatatcga tcggatcttg gaaatcgaaa
    22981 aattaacacg ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca ttgggagaag
    23041 aaagaaaatg ggtccattat ggtctaacaa gtacggatgt cgtagatact gcttatggct
    23101 atctcatcaa gcaagcaaat gatattttac gggaagactt gaaacgtttt acggaaatca
    23161 ttggcgaaaa agcaaaagaa cacaaatata cagtgatgat gggtcggaca cacggtgtcc
    23221 atgctgaacc aactactttt ggattgaaac ttgccacatg gtattcagaa atgaaacgga
    23281 acatcgaacg atttgaacat gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag
    23341 tgggaacgtt cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa caattgggga
    23401 tccgtccaca agaaatctct actcaagttc tccctcggga tctacatgct gaatatttct
    23461 cagcgatggc gcttattgct acaagtattg aacgttttgc aacagagata cgcggattgc
    23521 aaaaatccga aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg
    23581 ctatgccgca caaaaggaat ccgatcggtt cagaaaatat ggctggtcta gccagagtgg
    23641 tgcgcggaca catggtcact gcctatgaaa atgtcacatt atggcatgaa agagatattt
    23701 ctcattcttc agcagaacga atcatcttgc ctgataccac gattttattg aattacatgt
    23761 taaatcgttt tggcaatatc gttaaaaatc taacggtctt tcctgaaaac atgaagcgga
    23821 atatgtctgc aacatttggt ttgatttata gccagcgcgt tatgctgaaa ttgatcgata
    23881 agggaatgac aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg
    23941 acaaccaagt gcagttccgc ccgctgttag aagaagatcc aaccatcaca tcggtattat
    24001 ctaaagaaga aatagacgat gcctttgatt atcatcatca tttgaaaaac gttgacgtga
    24061 tattcaaacg agtaggtttg gaataaagaa gagtgagttg caaaattcaa ttaaaataga
    24121 atagcaaaaa taatcgagta gaggggaaag ccaactgacc ttccgcccct ctcacaccac
    24181 cgtacgtacg gacccgtata cggcggttca ataagttgag tgtacgaact ggtacttcaa
    24241 ggagatgtcc tcatatcctt ggcgggccag ttctttgtct gttaatgatc gatgaagaat
    24301 atgacttttg gagactgacc aaataccttt tcttgtattt gaccattcat acgctttcgc
    24361 tttatcgatc cctaaacgaa tcagattttt tcttttggtt cttggattct tccattgttt
    24421 ccaaatatat tgtctgattc ttcgcttcaa ccaaccgttt aactccctca tgaattcttt
    24481 catctcacca attccatagt agttgatcca accagtcatt agttgcttga tttcttttaa
    24541 aataagttgg atacttcttc cacggtttct tttagtgagt tgtttgagct tgcttttcac
    24601 acgctgtttt gcttttgcgt gagggcgaat tttaatacct ttctttgttg gcagaatgca
    24661 gaatccaaga aactttcgtt tcaatggact tcctaccgca ctcttttctc gatttaccgt
    24721 caactttagt tgttcttcga gaaattgaat ggatttcttc atcacacgct gtcctgctcg
    24781 tttgcttcta acatagatat tgcagtcatc ggcgtatcga acaaatttat gtcctagatt
    24841 ttctagcact tgatcaaatt cgttcaggta gatgttactt aacaggggag agagattgcc
    24901 tccttggggc gttccttctt cagttgttgt gatgagtcca ttttccatta caccactcat
    24961 taagtattga cggatcagtt ttaggacttg tttgtccttt actttccgct caatgaaata
    25021 catcagctta tcgtgattta cggtgtcaaa gtaggctttc atatcaatgt ccaccacata
    25081 tcggtaacct tgttcataat aagattttgc tttcttcact gcgtcatgtg cgctgcgatt
    25141 tgggcgaaac ccataactat tatctgagaa ttctggttca aatagcggtt gtaacacttg
    25201 attgattgcc tgttgaagca tacgatcaat aaccgtggga attcctagtt ttcttacacc
    25261 gccgtttggt ttcggtattt ctactcgcaa aacaggctga ggtttatagt tcccttggcg
    25321 taaattggat agtaattcct cgcggtgctc tttcaagtaa ggaagcagtt gatcgacggt
    25381 catcccatct gcaccagccg cccctttatt tctgactact tgaagatacg cttgattgag
    25441 attattccga tctagaattc tcccaaacag attttctaca ccatcttgtt tcctctgttc
    25501 accgcaagaa gtgctgcgca cttcatcata cccttcgtgt tccacactag ctctttgatg
    25561 atagccaagt tttcttgttt tctgcggtcg tcgcatcttc tcacacctcc taaattattc
    25621 aagattctta ttgttcagtc cttcgtaacg gtcagtgtcg ttactactat gacctcggct
    25681 gacttctcat gattcaccat catgtcgcca tgatcgttgt gctggtttgt tttcattcaa
    25741 cgcccacaac accctcatga gatctccccc ggtaagagtg aaaactttcc tcccatgtaa
    25801 ctgctagatt tactgtatag gtttcgggca gtattggact ttgtcttgtg atgaagactt
    25861 atccgaccta attcagcctt gtatctagtt tctgttcgtc agttcaggag tttgcgtccg
    25921 acttccttca gccacaacct cacgattgcc gccttgtctt tcgctaacag ttcctactgc
    25981 caagcctgta gtggactttc accaccaagt tttcacccat gcagggcgca caccaaaaaa
    26041 gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt atcaataata
    26101 ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga
    26161 ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca
    26221 gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat
    26281 aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt
    26341 ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga
    26401 tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca
    26461 gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac
    26521 cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc caaattttta
    26581 gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt
    26641 ttcgtttctg ataaaacacg aacattaaaa gaatataatg ccttaaatgt acatattttt
    26701 gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg
    26761 tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta
    26821 tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata
    26881 tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga
    26941 attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa
    27001 tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc
    27061 tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc
    27121 cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga
    27181 tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat
    27241 cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa
    27301 ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt
    27361 gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca
    27421 tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt
    27481 atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa
    27541 gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca
    27601 gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata
    27661 caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg
    27721 cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa
    27781 catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc
    27841 agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga
    27901 aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg
    27961 tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa
    28021 accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg
    28081 tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa
    28141 ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc
    28201 tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa
    28261 tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat
    28321 tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga
    28381 cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa
    28441 ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag
    28501 aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt
    28561 aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg
    28621 ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt
    28681 gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt
    28741 caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg
    28801 agttggcgga atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga
    28861 ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt
    28921 tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc
    28981 atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga
    29041 acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg
    29101 tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca
    29161 agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct
    29221 gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga
    29281 tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg
    29341 acttatattg acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat
    29401 gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa
    29461 aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga
    29521 cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc
    29581 attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga
    29641 atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga
    29701 tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca
    29761 agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg
    29821 gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc
    29881 aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg
    29941 taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt
    30001 ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc
    30061 tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac
    30121 accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt
    30181 caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag
    30241 tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc
    30301 cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag
    30361 tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca
    30421 tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca
    30481 atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg
    30541 agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac
    30601 tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt
    30661 atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat
    30721 ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa
    30781 ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg
    30841 ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc
    30901 ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta
    30961 ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt
    31021 tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag
    31081 atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt
    31141 gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt
    31201 gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca
    31261 tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat
    31321 gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg
    31381 aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag accagattca
    31441 aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa
    31501 gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca
    31561 agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac
    31621 attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa
    31681 ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt
    31741 gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa
    31801 gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt
    31861 caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc
    31921 ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa
    31981 aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg
    32041 ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa
    32101 aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt
    32161 agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga
    32221 acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt
    32281 tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt
    32341 ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa
    32401 tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa
    32461 aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg
    32521 aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga
    32581 ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga
    32641 aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg
    32701 gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact
    32761 gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc
    32821 ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg
    32881 agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact
    32941 tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga
    33001 gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag tccaagaatc
    33061 gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg
    33121 gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc
    33181 gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa
    33241 aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg
    33301 aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca
    33361 aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc
    33421 gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga
    33481 atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat
    33541 gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta
    33601 gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat
    33661 tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa
    33721 attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa
    33781 ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag
    33841 gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga
    33901 cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg
    33961 gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc
    34021 aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag
    34081 atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta
    34141 gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag
    34201 tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg
    34261 caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt
    34321 gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc
    34381 gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat
    34441 attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag
    34501 atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga
    34561 aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag
    34621 cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag
    34681 atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt
    34741 atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag
    34801 actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc
    34861 ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac
    34921 gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg
    34981 tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc
    35041 agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag
    35101 gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca
    35161 aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg
    35221 atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt
    35281 agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa
    35341 taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc
    35401 cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt
    35461 taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt
    35521 tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa
    35581 aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc
    35641 aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca
    35701 aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag
    35761 tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc
    35821 gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct
    35881 cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata
    35941 agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag
    36001 taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg
    36061 ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg
    36121 ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc
    36181 aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga
    36241 atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag
    36301 ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag
    36361 agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag
    36421 gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt
    36481 acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa
    36541 aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac
    36601 aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac
    36661 ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct
    36721 gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca
    36781 gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt
    36841 tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa
    36901 cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt
    36961 tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat
    37021 gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa
    37081 gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa
    37141 ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg
    37201 caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat
    37261 gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg
    37321 atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa
    37381 aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca
    37441 actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt
    37501 acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg
    37561 gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa
    37621 gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc
    37681 gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta
    37741 ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg
    37801 ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat
    37861 atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc
    37921 tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga
    37981 gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt
    38041 ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg
    38101 tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc
    38161 gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat
    38221 tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa
    38281 tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc
    38341 gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg
    38401 aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc
    38461 ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc
    38521 gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa
    38581 cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat caactattaa
    38641 aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa
    38701 aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc
    38761 tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt
    38821 caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt
    38881 caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt
    38941 tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc
    39001 atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa
    39061 tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat
    39121 gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag
    39181 tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac
    39241 agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt
    39301 atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg
    39361 ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa
    39421 ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt
    39481 tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta
    39541 taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata aatcctttgt
    39601 gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt
    39661 ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc
    39721 cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag
    39781 gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca
    39841 tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt
    39901 tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat
    39961 ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca
    40021 tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt
    40081 tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc
    40141 tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt
    40201 ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt
    40261 cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta
    40321 cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca
    40381 gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa
    40441 tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta agttcttcta
    40501 ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac
    40561 tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga
    40621 tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga
    40681 taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat
    40741 tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc
    40801 tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata
    40861 tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg
    40921 actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata
    40981 gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca
    41041 ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt
    41101 tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg
    41161 tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta
    41221 cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa
    41281 ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca
    41341 aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc
    41401 tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt
    41461 gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc
    41521 actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca
    41581 tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc
    41641 tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt
    41701 gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa
    41761 aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc
    41821 agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa
    41881 aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca
    41941 atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa
    42001 tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat
    42061 taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta
    42121 tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag
    42181 aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag
    42241 cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg
    42301 gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct
    42361 atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg
    42421 gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag
    42481 gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt
    42541 acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat
    42601 ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag
    42661 caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt
    42721 cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga
    42781 gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat
    42841 cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa
    42901 atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg
    42961 aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg
    43021 ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga
    43081 aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat
    43141 tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc
    43201 gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa
    43261 cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta
    43321 tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga
    43381 agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa
    43441 agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac
    43501 agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct
    43561 agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa
    43621 atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc
    43681 gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc
    43741 acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca
    43801 gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt
    43861 gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt
    43921 taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca
    43981 catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg
    44041 cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg
    44101 tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg
    44161 actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg
    44221 ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga
    44281 aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat
    44341 ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg
    44401 gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg
    44461 ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg
    44521 tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa
    44581 cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa
    44641 attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa
    44701 aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg
    44761 tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc
    44821 gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag
    44881 ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt
    44941 ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag
    45001 ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt
    45061 atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga
    45121 cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt
    45181 ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt
    45241 taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga
    45301 aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag
    45361 cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt
    45421 tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag
    45481 gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa
    45541 caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat
    45601 tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg
    45661 atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca
    45721 tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag
    45781 gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc
    45841 tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa
    45901 gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc
    45961 gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc
    46021 tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta
    46081 aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta
    46141 tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa
    46201 tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac
    46261 atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat
    46321 atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt
    46381 ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag
    46441 ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct
    46501 aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt
    46561 gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca
    46621 acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg
    46681 actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc
    46741 atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg
    46801 acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa
    46861 gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc
    46921 gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat
    46981 acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat
    47041 ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac
    47101 acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta
    47161 ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg
    47221 cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca
    47281 cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa
    47341 aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag
    47401 gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca
    47461 atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt
    47521 ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg
    47581 gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt
    47641 ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag
    47701 aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg
    47761 agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa
    47821 aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa
    47881 gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag
    47941 caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac
    48001 cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac
    48061 cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat
    48121 tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa
    48181 aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg
    48241 atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg
    48301 atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa
    48361 tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga
    48421 tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa
    48481 ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg
    48541 cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct
    48601 ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg
    48661 gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt
    48721 acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa
    48781 agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca gcttggattt
    48841 aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa actaagaaaa
    48901 aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact
    48961 gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa
    49021 aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact aatgctgtta
    49081 atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc
    49141 tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag ttttattcac
    49201 tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg
    49261 gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc
    49321 ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat
    49381 ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac
    49441 caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga
    49501 caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca
    49561 ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca agctttttta
    49621 tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca
    49681 caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa
    49741 cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat
    49801 ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa
    49861 aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca
    49921 caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca
    49981 gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta
    50041 atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc
    50101 taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt
    50161 aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca
    50221 actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt
    50281 tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca
    50341 atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca
    50401 cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg
    50461 ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag
    50521 accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg
    50581 cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa
    50641 tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc
    50701 tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg
    50761 ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc
    50821 cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc
    50881 tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag
    50941 ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg
    51001 gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg
    51061 ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg
    51121 tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg
    51181 cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac
    51241 gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa
    51301 atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac
    51361 gaaacaaagt catggcttat caatttaaat tgccggatat cggtgaagga atcgcagaag
    51421 gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat gatacattac
    51481 tagaagttca aaatgacaaa tcagtagaag aaatcccgtc acctgttaca ggaacggtta
    51541 aaaatgtgat tgttccagaa ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg
    51601 atgcaccagg acacgaagac aatgaagggg acagtggtgt agccgcagaa tcccaaactc
    51661 cagctaaacc ggctgctgaa cccactgttg atacagaatc agctggatcg tcaagcgaag
    51721 gagtcttcca atttaagtta ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca
    51781 aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta gaagttcaaa
    51841 atgacaaatc agtagaagaa attccgtcac ctgtcacagg gacagttaaa aacgtgattg
    51901 ttccagaagg cactgtagca aatgttgggg atgtattagt agaaatcgat gctcctggac
    51961 acaatagtgc accgtcaact agtgcgccat cagctgaagc accaaaagaa aaagtggaaa
    52021 catcaggttc agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta ttggctatgc
    52081 catctgttcg tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta actgcaactg
    52141 gaaaaggtgg cagagtaaca aaagaggaca tcgaaaactt cttggcaggt ggtccaagca
    52201 gtgcaccagc gaaatcagaa gcgccagaag cggcagcacc gaaagaagca gctcctgcag
    52261 cagaaagcaa accagctgca ccagcaaaac cattcaagtc caacttagga gacttggaag
    52321 aacgtgtagc aatgacgcca acacgtaaag ctatcgctaa agcaatggtc aacagtaagc
    52381 atacagctcc acacgtgaca ttgcatgatg aagtagaagt gtctaaacta tgggataatc
    52441 ggaaacgatt taaagaagtt gctgctgcaa atggtacgaa acttactttc ttgccatatg
    52501 tcgtgaaagc tttaacagcg actgttaaga aatacccagt attgaatgca tctatcgacg
    52561 atgcaaacca agaaatcgtt tataaacatt actacaatat cggtattgca actgatacgg
    52621 atcacggact atatgtacca aatgtgaaag atgcagaccg caaaggaatg tttgctattg
    52681 cagacgaaat caatgagaaa gcaaaattag ctcatgacgg caaactatct gcagaagata
    52741 tgcgcaatgg aacaattacg atcagcaata tcggatctgt aggtggcggt tggtttacac
    52801 cagtcatcaa ctatccagaa gtagctattt taggtgtagg aacgattgca caacaaccaa
    52861 tcgtaaatgc tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg
    52921 atcaccgtat cgtagatgga gcaactgcgc aacaagcaat gaataatatc aaacgtcttt
    52981 tagctgatcc agagctatta atgatggaag gatgataaac aaatggtagt tggagatttt
    53041 gctgtagaat tagatacagt tgtaattggt gcgggacctg gtggctatgt agctgccatc
    53101 cgcgctgctg aaatgggaca aaaagtagca atcattgaaa gagagtatat tggcggtgtt
    53161 tgtttgaatg tcggctgtat tccttcaaaa gcactgatag ctgcaggaca ccattatcaa
    53221 gagtcactag attctgaaat gtttggagta actagtgaaa acgtcaaact agattttgca
    53281 aaaacacaag aatggaaaga aaacaaagtt gtccatacgt tgacttctgg tgttggtttc
    53341 ctattaaaaa aacataaggt tgaaacaatc gaaggagaag cgttcttcgt agatgatcac
    53401 acactacgcg tcattcatcc agattcagct caaacttatt cgttcaataa cgcaatcatc
    53461 gcaacaggtt cccgtccaat cgagattcca ggatttaaat tcggaggacg tgtgttagat
    53521 tctactggcg gattagcatt gaaagaagta cctaagaaat ttgtgatcat cggtggcggt
    53581 gtgattggtg ccgaattggg cggagcgtac gcaaacttag gagcagaagt aacgattctt
    53641 gaaggctcac cacaaatttt accaacttac gaaaaagatc tagtaaaatt agtagaagat
    53701 gatttcaaga aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat
    53761 aacggtgaca gcgtgacagt gaaatatgca gtggacggga aagaagaatc cgttactgct
    53821 gattatgtga tggtcactgt aggtcgtcgt ccgaatacag atgatatggg cttagaacaa
    53881 gcgggtgtcg aagtaggaga acgaggattg atcacggtgg ataaacaagg acgtacgaac
    53941 gtacctaaca tctatgccat cggtgatatc gttccagggg cggcattagc acacaaagcc
    54001 agttatgaag caaaaatcgc tgctgaagca atctctggca aaaaagtagc tgtagattat
    54061 aaagcaatgc cggcagtagc cttcactgat cctgaattgg cttctgtagg tatgacgatc
    54121 aaagaagcaa aagatgctgg tatcgaagca aaagcatata aattcccatt ttcagggaac
    54181 ggtcgggcat tatcgcttgg taaaacagaa ggattcatcc gtctagttac gacgatcgaa
    54241 gataacgtgc ttattggcgc gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa
    54301 ttagctttag caatcgaatc aggaatgaat gctgaagata tcgccttgac gattcatcct
    54361 catccatctc ttggcgaaat cgtcatggat gcttcggaat tagcattagg tttaccaatc
    54421 catatttgat ttgaataata caggcgaaac gcaactgaaa aatcagttgc gttttgtttt
    54481 ttagaattat caataatatt attatgtaaa cttaaaaata tgacaacttc ttggtttatt
    54541 accacttcct atgctacact gaagaggaat atgaaatagg agagttgaag atgcaagaat
    54601 atcagtatcc acttgattta gattggacaa cagacgaaat ggtcattgta atgaagatgt
    54661 gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt ttgaagactt
    54721 atcagcagtt taaaactgtt gtcaaatcca ttggtgagga aaaacgattg ggacgggaat
    54781 ttgaaaaagc ttccggttat tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa
    54841 acaaaacttt gagaatgaac gggtgagata aatggaacag atgattgatc atatcaagtc
    54901 ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa caaacattaa caatctcgca
    54961 aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga aacatccaga attttctaat
    55021 cgaaaaaatc cattcctttg atccagaggc gaaaatattg gcagaagaag aagggtgtaa
    55081 tcaattatcg gatctcgatg gaagagtctt tatcatcgat cctattgatg gcaccttgaa
    55141 ttttgtgatt gaaggggaaa atttttgtat tatgctcgct gtttatgaat ccggcgttgg
    55201 aaagcttggt ttcatttatg acgtgatgag aaatgaactt ttttggggtg gaaaaggaat
    55261 aggcgtgtat aaaaatgatg agccattgcc tagaccagca aataaatcct tatcggaagg
    55321 cttacttggt gtgaatagtt atcttttcgg tcataataaa tatggaatcc gagaaattgc
    55381 ggaaagcagc atggggacac gtatgtacgg atgcgctggt cttgaactag tggcaatctt
    55441 gaaaggaaac cacattgctt atatttcaaa ccttagccct tgggattatg cagcgggaaa
    55501 tgtcttattg gaagagtttg gtatggtaac aagcggtctt tttggtactc ctttgacatt
    55561 tgaaaaaagg gaatactatt tagcagggac ttcttgtgca tatgcagaaa tcgcagacct
    55621 tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca caaaaatgat aaaaacgagc
    55681 aaaagattaa ttttctcgaa aaaatgaaaa cattttacta tatttcatga aaaaagtctg
    55741 ctataattag cagtcgagta aaatttgccc tgtcataaag aaccaataaa aaaggagtta
    55801 gttacattga attatagaaa cgatatccgt aacgtcgcaa ttattgccca cgttgaccac
    55861 ggaaaaacaa cattagttga tgaactatta aaacaatcag atacattaga cgcacatacg
    55921 caattgcaag aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt
    55981 ttagccaaaa acacagctgt agactataaa ggtattcgcg taaacatcat ggatactcca
    56041 ggacacgcgg acttcggtgg cgaagtagaa cggatcatga aaatggttga cggcgttgtg
    56101 ttggtcgtag atgcgtatga aggaacaatg cctcaaacac gttttgtatt gaaaaaagca
    56161 ctggaacaac atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt
    56221 cctgagcatg tcgtagatga agttcttgaa ctattcatcg aattaggagc agatgatgat
    56281 cagttagact ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag cttgtcagat
    56341 gatccagcag atcaagaacc aaccatggca cctatttttg atacaattat tgaaaaaatc
    56401 cctgctcctg tcgataacag cgacgaacca ttacaattcc aagtttcatt acttgactac
    56461 aacgattatg taggtcgtat cggaatcgga cgtgtattta gaggaacaat caaagtagga
    56521 gaccaagtag cattaattaa attagacgga acagttaaga aattccgtgt aacgaaatta
    56581 tttggtttct ttggactaaa acgtctagag attcaagaag caaaagctgg agacttgatt
    56641 gctgtctcag gtatggaaga tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa
    56701 gatgcgttac ctatccttca cattgacgaa ccaacattgc aaatgacttt cttagttaat
    56761 aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt
    56821 ttgatggcag aacttcaaac agatgtatcc ttacgagttg aaccaacaaa ctctccagat
    56881 gcatggacag tttctggtcg tggagaattg catttgtcta tcttgattga aaacatgcgc
    56941 cgtgagggat acgaattgca agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt
    57001 gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac cagaagaata tatgggtagt
    57061 gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag atatggtcca tacaggaaac
    57121 ggacaaatcc gtttgacatt cttaacacct gcccgcggtt tgattggtta ttcaactgaa
    57181 ttcctttcaa tgacacgtgg ttacggaatc atgaaccata cttttgacca atatctgcca
    57241 atgcttcctg gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat cgatacagga
    57301 aaagcaacga cttattctat catgagtatc gaagaacgcg gtacagtctt cgtagaacca
    57361 ggtacagaag tatatgaagg aatgattatc ggggaaaaca gccgtgataa tgatttgact
    57421 gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa agaccaaaca
    57481 tctgtcatca aaaaaccaaa acaattgacg ttggaagaat ctttggaatt cttgaatgat
    57541 gatgaatatt gcgaagtaac accagaaagc attcgcttga gaaaacaaat tttagagaaa
    57601 aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg taaaaggatg aagagagaaa
    57661 ccagcttgtt ataaaaaata gctggtttct ttttttgtcc aactcttttc tgtggagaag
    57721 tcatctttgt caatggacat aaattcagaa aatacgccga aagacgttta aaagactttt
    57781 aaaaaactta tttctagaga gtttttgttt cataataaga taatgtttca cgaaaggaga
    57841 tcaacttgtc cttcgaagaa aatcgaagta aaatggcaag agaaaggagc gggaagatga
    57901 acgacttatt aaaacaatac tttggctatg atgagtttag acctggtcaa aaagaaatta
    57961 tccagaaagt cattgatcag gaaaatgtat taggaatcat gcctaccgga agtgggaaat
    58021 caatctgtta ccagcttcca gctttactat tagatgggct tactgtggtt gtgtcccctt
    58081 taatctcttt gatgaaggat caggtggacg cggcgaatca attaggtata cccgccactt
    58141 ttatcaatag ctcgttagat ggttacgaaa cagctagacg cttccaagaa atcgatagac
    58201 agcagtaccg tttactttat atcgctcctg aacgttttat catgccggat tttatccaag
    58261 caatgaagcg atggaacgtc cgtatgatcg ctattgacga ggcacattgt atctctcagt
    58321 gggggcatga tttccgccca agttatttac agatggcgaa ccagctagat caactgccaa
    58381 atcgtcctgt aattgttgct ttgacagcta cagcgactgt gcaagtagct gctgacataa
    58441 aaagactttt aaaaattcca gaaaacaacc atattcagac tggttttgaa cgagaaaatc
    58501 tacgatttca agtaattaaa gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga
    58561 tcaacaaaaa tcaatctggt atcatttatg cagcaacgag aaaagaagta gaccgtctct
    58621 atcatttgct taaaaaattc gatttttccg tgggtagata tcatggtggt ctaaatgaga
    58681 atgagcgaac agaaatgcaa gaagcctttt tatacgatcg cttgcagctg attgtagcga
    58741 caaatgcatt tggtatgggt ataaataaaa gcaatgtccg ttttgttatc cattatcaaa
    58801 ttcctggctc tctagaagca tactatcagg aagctggtcg ggcgggaaga gatggtcttt
    58861 ccagtgaagc catcctgctt ttctctcctc aagatatcca agtccaaaag ttttttatcc
    58921 agcagtccca aagagaagaa gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga
    58981 cagaatacgt gtatattgag tcctgtcttc agcaatacat cctaaactat tttggcgaga
    59041 caagcagtcc gtgcaaccgt tgtggtaact gtttagatga cagagaaata gtagaagtaa
    59101 cgacagaagc acagatggtt ctttcttgct taaaacgtat gggtgagaac tatggtaaac
    59161 aaatgctgat gaaagtctta gcaggatcga aagaacaaaa gctgcaagct ttaggttttg
    59221 gacatttatc tacctatgga ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa
    59281 ttgaatattt gatatcgaat ggctatcttc tgaccatcaa tggtgagtat cctgttttga
    59341 aagtaacaga aagagggata caagtattga aaggacaaga atctgtttat cgaaaggaac
    59401 ccaagaaagt ccagcagcta tcggatgaag aaaccgatac actatttgaa gtgctgcggg
    59461 aactgcgaac ggatctagca tcagaagcag gcgttccccc gtatgtcgta ttttcagatt
    59521 ccacattgaa agaaatgagc cgaatacgtc catcaagcag attggagatg cttcagataa
    59581 aaggagttgg ccaaagcaag cttgataaat atggcgaagc ctttttatct cggataaaaa
    59641 atgcggactc taacgttctg gataagtaga aagaacgaat acgtaaaaat gaataaaaac
    59701 aactggttaa atctgttttt tttgctcctt atgaaaatca aatatgttat aattaaggaa
    59761 ttagaatggg aggtaagcac agatggattc tttgtcgaaa aaacttgcga tccaagtatt
    59821 aaatgatgaa gcagaacgga tcaaacgatt aatccgaaat caaaacaaca gtctctgtat
    59881 ctcacaatgc aaagccttcg aagaagtggt agatacacag atgtacggct tttcccggca
    59941 agtcggtttt gctgtccgta taggagtaat cggaaagaac gagggccagc tgatgttaag
    60001 tgagctagaa cgagaattga atcagttata tagtgaggtt taccaagaaa tttatgataa
    60061 aaaagaagtt ggcaaggagg aatagacttg cctaagaaag tgaaaaaaag gcatctatta
    60121 gactatagca tcttgattcc ctatttaatt ttatgtgtat tgggattgat catggtatat
    60181 agttctacgt cgtatctatt attagaaaac ggacaaaatc cgtccgcatc agtcattaac
    60241 cagagtattt tttgggtgct tagcttgatt gctatcgcac tactttataa aatgaaaaca
    60301 gatgttttga aaaaccagcg tttgatcatg gcagccatcg cagttttgac gattttgctg
    60361 ctgattgttg tgttttttgg taaggagatc aatggggcga aaggttggct gcaaattgca
    60421 ggattctcga tccaacctgc cgaataccta aaaatcattt ctatctggta cctgtctctg
    60481 actttatcta aaagacaaaa cagtgtacaa aaagattttc ttgggactgt gaaacgtcca
    60541 cttgcaatgg ttatcggact tactgcatta gtcgctattc ttcccgattt tggtaatgcg
    60601 gcagttatct ttttgattat tttggttctg cttttagcca gcggagtcaa ttatgtgtat
    60661 actttgattg taggagtagg aggcttttgt ctaagtactt ttacgatctg gctgatcaat
    60721 atcacaaacg gaaagatctt acccggacgt ttacagtata tctataatcg atttgctatt
    60781 taccaaaatc ctttctcaga cgaattaaat aaagggcatc aactagtcaa cggctactat
    60841 gccatgttca acggaggact atttggcagg ggattaggta acagtatcca gaaaaaagga
    60901 tttttacaag aagcacaaac cgattttatt tatgcaatcg tagtggaaga actgggagtg
    60961 attatgggga ttttgatatt agcgttgcta ttctttatga ttgtgcgcat tgtccttgta
    61021 ggtatccgtt caaaagatcc gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt
    61081 ttgatccaag tatttgtcaa tcttggagga attactggcg ttattccttt gacagggatc
    61141 acgttcccat ttttaagtca aggaggctcc agtttgctga tgctttcgat ttgtgtcggc
    61201 tttgtcctca acatcagtgc ggatgaaaaa cgcaagagct taggtttata ataaaaaaag
    61261 atgtaggagt ggttcgatga aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg
    61321 tgtatttcgt gcttgtacag aattaggaat taaaactgtt ggaatctatg cagaagaaga
    61381 tgaatattcc gtacaccgtt tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa
    61441 accgattgat gcgtatctag atattgaagg gatcatttct atcgcgaaag aatgcggagc
    61501 tgacgctatt catccgggtt acggtctttt gtcggaaaac ttgaattttg cgcaacggtg
    61561 cgaagaagaa ggcatcattt ttgttggacc caaactgcat catttagata tttttggaga
    61621 taaaatcaaa gcaaaagctg cagctatcga agcggggatt gcttcgattc caggcacaga
    61681 tggaccaatc gcttctatag acgatgcctt ggagtttgcg aaacaatacg gttatccgat
    61741 tatgatcaag gcagctctag gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa
    61801 gagtgcaaga gagggctacg aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga
    61861 tgaagtgtat gttgaaaaat acatcgctaa tcctaagcat atcgaagtac agattctcgg
    61921 ggatacgcat ggaaacgtca tccatctttt tgaaagagat tgttctgttc agcgccgtca
    61981 ccaaaaagta gtagaagtag cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat
    62041 ctgccaggca gctgtccaat tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga
    62101 atttcttgtt gaaggagatg atttttactt tatcgaagtc aaccctcgcg tccaagtgga
    62161 acatacgatc acagaaatga ttacagatat tgatatcgta acaacgcaac tgttgattgc
    62221 tcaaggtttg gatctgcata aagaaatcgg attgcctcag caagaaggaa ttaaattaaa
    62281 tggttcagcc attcaatgcc gaatcactac agaagacccg ttgaataact ttttaccaga
    62341 cacagggaaa atcgatacgt atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg
    62401 aaatgcttac gcgggctatg tagtgacacc gtattttgat tcattgctag taaaagtatg
    62461 tacgcatggt gctacctttg aaacagccat tcagaaaatg gaacgttgct tgaaagaatt
    62521 tagaatccgt ggagtgaaaa caaacattcc atttatgctc aatgtcatca cacatcctga
    62581 attccaatca ggaaatgcaa aaacaacctt cattgatagt acagcaacat tatttgaatt
    62641 tcctcgacta cgagaccgcg gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa
    62701 tggttttcca ggtatcgaaa gtggcgaaaa accattttat gaagaaccac gtatgcctaa
    62761 agatctaatc acaagaactg attacgtaac ggctaagaat gtattagatg cgaagggtgc
    62821 cgatgctttg gtcgaatgga tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt
    62881 tagagacgcc catcaaagtc tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat
    62941 tgcacgtttg actggagaag gtcttcctga actcttttca agtgagatgt gggggggagc
    63001 aacatttgat gtggcttacc gctttttgaa tgaagatcct tggcaacggt tgagaaaaat
    63061 tcgttcttta atgcctaata cgttgctgca aatgcttttc agaggttcaa atgccgttgg
    63121 gtattctaat tatcctgaca atgtattggt ggaatttgtc aaagaagcag cagcgcaagg
    63181 aatcgatgta tttcgaatct ttgatagttt gaactggact ccacaaatgg aaaaaagtat
    63241 ccaagctgta cgggatactg gtaaaatcgc agaggcagct atttgctata ctggcgatat
    63301 caacgatcca agtcgggcaa aatacaatgt tcagtattat aaagacatgg caaaggaact
    63361 agaacaactg ggtgcgcata tcattgcaat caaagatatg gctgggctgc ttaaaccgca
    63421 ggcagcttat cgtttgatta gtgaattgaa agaaacgaca gatctgccaa tccatctgca
    63481 tactcatgat accagcggga atggaatcat cacttattct gctgcttcaa aagcaggagt
    63541 agacatcgtt gacgtggcaa tgagtgcaat gagtgggaat actagtcagc ctagcatgag
    63601 cagcttgtat tatgcactgg tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc
    63661 acagaaatta aaccattatt gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg
    63721 cttgaatgct cctgaaacag aagtatatat gcatgaaatg ccaggaggac agtattcgaa
    63781 tctacagcaa caggcaaaag ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat
    63841 gtatcatact gtcaacttga tgtttggcga tatcgttaaa gtaactccat catctaaagt
    63901 agttggagat atggcactat ttatggtcca aaatgatctt acagaagaag atatttatga
    63961 aaaaggcgaa acactaagtt tccctgaatc tgttgtgact ttcttccaag gagaactagg
    64021 gcaacccgtt ggcggatttc ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc
    64081 attgaatgaa cgtccaggtc ttttggctga atcagtcgac ttcaacgaag taaaaaaaga
    64141 attggcagaa aaaattggct atgaaccaaa acaagacgaa gtattaagct atttgatgta
    64201 tccgcaagtc tttttggact atcaaaaagc atataatcaa tttggcgatg ttacattact
    64261 ggacacacca acatttttcc aaggtatacg tttgggtgaa acaatcaatg tacaaatcga
    64321 acgcggaaaa acgcttatca ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa
    64381 tcgcgtactg ttcttcaatt tgaatggaca aagacgtgaa atcactatca aagataattc
    64441 gatcatcagt acggtccaaa cccgattgaa agctgaaccg acaaaccgag aacaaattgg
    64501 agcaacgatg tctggttctg ttttggatgt attagtgaaa aaaggcgata acgttaaaaa
    64561 aggtgatacg ttgatgatta ctgaagcgat gaaaatggaa acggccatcg aagcacgttt
    64621 tgacggagaa gtcgcacatg tatatgtgtc ttcaggagac actatttcgt ctggtgacct
    64681 tttgattgaa gtgactgaga aataataagc gaaaggtggg gaaacatgaa aagactgggt
    64741 ttgttttggc tgattctgct attagttatt gtggcattct acatggaacc agtcttgttt
    64801 ccgccaaaac ttgaagtgaa ggaaaactct gatcaacaaa tatcaaacca aatgacaacg
    64861 tggaaatacc aggagcttaa agcaactggt ttttctactt atatcggaaa accgatcgat
    64921 cagctggaaa aggattttgg ccaagcctat gatcgattaa ctagcggatt tggttttgaa
    64981 acaagatatt atgaaaataa agctagtcat ctcacatttg aagcgaacat ccaagacagc
    65041 agagtcacaa ctgttaaagt actgcaaaca gggcctgctg atatcgcgcc gttttattta
    65101 gggatgacga tgcaagatct tacgaaaatc actactatct atactaattt cacttttgag
    65161 taccatgata cagaagttgg aatcgaacta atggaagagg atatgaatta tcgtccgcta
    65221 attgcttttg ataacgacac ttttgctatt ttattttttg atcaaacaac aaacggccta
    65281 ttttcagttg tgtatttaga taaagatagt cttttgaaac tattgccata tcaagtgttt
    65341 ggtgaaggcc ttccacatta tcaagcagat aaatcggctg attgggaatc aatcaatcgc
    65401 gttaaagaac gaaaaagtac gactttatta aatatgctta gaagagaaga cgaacttccc
    65461 atatacaatc aaacacttgc ctttcaaaac cacagtcagc tgctactaca tgattttttg
    65521 acaaggcctg aggaaatatt gtcagaggaa cgtatggctg attggcaaaa atcgctaggc
    65581 agtgctcaaa cgacagtgaa attctcactg acgaatgatg aattggagaa gttaatcagt
    65641 aaacaaaaat tagaaaatac tagtgggcta tttattcatc cggcgattga cgctactttt
    65701 tcgtttttat attggtacag cgatccttac aaccatgacc gtttcatgga cccgggaacg
    65761 gattctgtag gcattgcttt ttctaaagaa aatatgttag tcttattgca agaagaaaac
    65821 ggagaaagta gtgatagcag tgatcgttaa cgaagagctg tttcaactgg aggatcaatg
    65881 cctccaagtc gctgaaatga taaaaaagag taaaacgatg gcttcttatc ttgaacataa
    65941 acagtcaatg gaagaatctg caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa
    66001 agaagcattt gaaaatgtag cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa
    66061 acgagctgtt cgaaaagcaa aaagacagct tgatatggat gagcatgttg cagctttccg
    66121 atatagtgaa acacagctgc aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga
    66181 agtttctaat gaaatcaaag tagatgcagg tagcccgttt tttgaaacga aaaaacactt
    66241 tggatgcgga ggtaattgtc atgcaattag acaatagtga atttgaaata acaaaacgac
    66301 gcgggctgat cgtatgggtc tacagcttaa aacaattaaa gaatctgaaa aagttcggct
    66361 tgttgcatta tgtctcaaga agaatgaaat atgtcgtgct ttacttaaat gaagagacat
    66421 tggaagaaac agaagaacga atccgtaaat tacattttgt tcgaaacgta gaacgttctt
    66481 accgaccgga tatcgaaatg aattttgccg aaaaaatagg aaacaaaaca gtattaaaag
    66541 acgaaggttt cgaaattgaa gagctaaata cagaaatccg attagctgaa catctttaaa
    66601 aatctcttat gagcagtttt taaataaaaa ataacttgga taacgatcga tttttgatca
    66661 ttgtccaagt atttttttgg tatactagta ccagtaccat ttctaaggag tgagtagatg
    66721 cggataatat caggaaatta tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc
    66781 agaccaacta cagataaagt aaaagaatcc atattcaata tgattggacc atattttgat
    66841 ggagagacag tattggatct gtttgcagga agcggtggtt tagctatcga agcagtatct
    66901 agaggctgca gtcatgcggt atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa
    66961 gaaaacatcg aaattacgaa agaaccagag aaatttacca caataaaact tgatgcggac
    67021 aaagcaatcg aacggctagc agcggagaaa caacaattcg actacttgtt tcttgatccg
    67081 ccatatgcaa aacaaaaaat aatcgatcaa atccataaaa tggaagaact gggacttttt
    67141 tctaaaaccg cagttatcgt atgcgaaacg gataaaacag tcgaattgcc agaaactgtg
    67201 gctggattcc atcaaataag aaaacagatt tatggcataa cggccgtaac tatctataga
    67261 aaagagggaa atcaatgaaa cgtgcattat tcccaggaag ttttgatcct tttacaaaag
    67321 gtcacttaga tacggtggaa cgtgcggcta aattattcga tgaggtagta attggtgttt
    67381 ttatcaatac ttcaaagaaa agtttgtttc ccccagaaga aagaatgaca ttgatcacaa
    67441 aagctgtttc tcatcttcct aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag
    67501 aaacagcaaa agagattggt gcagatgcgt tgatcagagg catacgaagt atcaaagact
    67561 ttgaatatga acgagagatt gctcagatga atcatcatct atatgatgaa ttggaaactg
    67621 tcttcttatt agcaaaacca gaatatagtc acgtgagttc cagtatttta aaagaagtac
    67681 tgcactttgg cggagatgtg agcagctact taccgccagt gatcaacgaa gctttagctg
    67741 gaaagcgtga atcaaatgaa agctaaacaa tggttgaaaa cagtactgat tatcatattt
    67801 ggaatgttga tcattggatt tgtagtagtt cccttgccgt attatgtaga atcaccggga
    67861 gccacgatcg atctgaaaga actaattacg gtcaatgata aggaagataa aggttctggg
    67921 tctttctctc ttacctctgt tggtatcagg caagctacag gtttcttagc tttaaaagct
    67981 aaaatgaccc cttttgaaga attgatcagt gaagaagaat tgactggtgg ggcgacaaat
    68041 gaagaatata atcaaatgca acagtattat atggattctt cccaaaatgc agcaatcgaa
    68101 caagcgttaa aacttgcaga caagccttat aaaatggaat tcaaaggcgt atatgtcatg
    68161 agtgtagaga aaacatcgaa tttctttggc aagctttcag ttggagatac agtaacaaaa
    68221 gtagacggga aaagctttca aagtacagaa gcatttatgg attatgtaaa aagccaaaaa
    68281 gttggacaaa caatcgaaat cagttatttg cataatggtg aagaaaaaac agcttctggt
    68341 gatctgatcg agttaccaac agataaaaag gcaggaatag gtatcacgct tactgaccat
    68401 accgagatcg aatcagatac agatgttcgt attaattctg gctctatcgg tgggccatca
    68461 gcgggattga tgttcacttt agaaatctat gaacaagtca ctggtaaaaa cttgcgacat
    68521 ggtaagcaaa ttgcaggaac tggaacgatc aacagtgaag gagaagttgg ccgaatcgga
    68581 ggaatcgata aaaaagtggc aagtgcggat aaagctggcg tagagatttt ctttgcacca
    68641 gatgatacga tttcagacga tgttaagaaa gaatatcctg aaataaaaac caactacgaa
    68701 gaagcaaaag ctgccgctaa aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca
    68761 gtacaagaag cgcttgacta tctagagaaa atgaaatgaa actaaataat taaatagggg
    68821 ttgtgacacg agttctgtca caacccctct ttttcgtcaa aaatcttcac gataaaaata
    68881 aaaacaaaag aaaaaattga cgtaattaga tgttgaaggg acgattttgc cagagctcac
    68941 tgtttcttca attttaaatg aaaggagtaa tgaatgattt ttactggtca ttctttgaaa
    69001 aaagaacaat gatagaaaaa atcagcaaga aaatactagc ttcacccaaa ttgatcattt
    69061 ggatgtctat gatagttctt atcttttgta tcgcaagtgg ttttttatta tcaaatttgc
    69121 tattttatag agaaacagaa gagactgctg caaaagaaat actttcttcg tttagttcca
    69181 gtggagaaga tccatcagct gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca
    69241 tgtacaccga tatcaaagga agtgtgaagg agcctggtat ctattccttt tcttcagaag
    69301 aacgggtcta tgatgtacta aagcgtgcag gtggattatt agaagaagcg gatagcgatc
    69361 gaatcaactt ttctgcgaag atagaagatc agcaagtcct atatatccct gctgtaggtg
    69421 aagagctgcc agaacatctt gatcagttgg catcctcgga aggaaaacag cctacagcag
    69481 attccgagcc tcccaaaata aatatcaata cagccactcc atcagaactt cagcagattc
    69541 ctgggatcgg atcagtcaaa gcgcaagaaa tcattcgatt tagagaagaa aacggatcct
    69601 tccagaaagt ggaagacctg caggagattt ctggtatcgg tgaaaagaca gttgaaaaac
    69661 taaaaaactt cgttacaatt aaatagaatg attcaaaatc gactggcacg agcaataaat
    69721 ggacgctaat ggatactaag aataatccaa ttctaagagc ttggcaggac gaggaggtag
    69781 tatttttgga taggaaatag cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg
    69841 aatgtctatc tccaaaataa aatatctggg attcatatta tgaatacggc tgtcagcggc
    69901 aataacagtc aagctctacg agaacgcttt gccaaagatg tacttagcta tacagcagat
    69961 aaagtattta ttctcattgg gacgaatgat ctagcagagc ataaacaact ttctaaagaa
    70021 acgtaccaaa aaatctgcag tggatgatag ttcagctgaa aaaagactat agttctgatc
    70081 atgctttctt tatcacgcca ccagcagtcg atgagacaaa acaaaaatat cggaataaca
    70141 agttgatcaa agaatatatc tctgttctca cagaagtagc aaaacgccat gattgtgaag
    70201 tatcgatttt tatacggaat ttcttgaaca aatggattcg aagaattttt ataatgggct
    70261 aatggatgac ggacttcatt tcggcagtga agggtaggat cttttgtcag acaggatgat
    70321 tcgttatatt taaagaatat tttgtttaag caatgagtac tttttaatca acttgaaaaa
    70381 acggaggaat ataaaacatg ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa
    70441 aagttactga taagtcacat aatgaacgca ttccttggga ccagtacttt atggctcagg
    70501 cagtactgtt atctttacgt agtacgtgta cacgtttaga agtaggagcg acactcgttc
    70561 gagaaaaaag gatcattgct ggcggctata atggagcagt cagtggagat gtccactgca
    70621 tagatgaagg gtgttacgtc gtggatggac actgcttgcg aacgatccat gcagagatga
    70681 atgcactcct acagtgtgca aaacttggta tcccgacgga aggttcagaa atctatgtca
    70741 cacattttcc atgtttggcc tgtacaaagg ctttgttgca agctggtgtg aaagggatca
    70801 attatctcca tgactatcga aatgatcctt atgcccaagc gttgatcgaa caattaggtg
    70861 tatctgtaca ccaagttgct ttagactcac agcatttttc aaaacttcaa aatgaattaa
    70921 caggaaaaca tgtctagcgt tttagaaagg catgttcgaa atcggttgat ttttccggca
    70981 attacgtcga tgattatctg tttgttgtat tacactgaca attggggtta tcgtttgctt
    71041 cttgccggaa tttttttatg gttttcgcta aaagaaggac gttttatcgc gtacttgtgt
    71101 attttgtgta gcataacggt atccatttct tataggctac aagagtattc atttttagag
    71161 gaaaaaacaa catctgcgca aattactgta tttacagata cgattcgtgc aaatggtaat
    71221 tttttgacaa tggaaggaag gttatcagag aataaacaaa agctccagct ttcttatcaa
    71281 gtaaacacag aagcagaatt gaaaacatgg cttaacttcc gaggaaaggg aatagaattg
    71341 actgctgaag gcgagttcat ctctccagca aaaaagcgga atctttataa ttttgaccaa
    71401 gaagcttact tcaggaacca tagaatttct ggacggttcc aaattcaaaa gattgaacag
    71461 atcaccatgc aaaatcactg gcgaaactgg cttcagagaa aaagaggatc ttttatctcc
    71521 tttgtccaat ctgaatttcc gcaaaaaaca agtgtatata ttaattcttt attatggggc
    71581 tataaagatg caggattcag agaaccagaa gaaattttta gggagagtgg tttgctgcat
    71641 ctgttcagtc tatcgggtct acacatccaa ttttatttag ggtggctgta ttatttgttt
    71701 cgacgtatcg gatggccact acacgtcagt attattccgt tgagtctact aactatctgt
    71761 tatgtttcta ttgcaggaag ttctatttct gttttgcgtg ctagtttgct gtttttattg
    71821 cgattgctag tcaaattgct gaataagcat tattccacca tggacttatt tgcagttgtc
    71881 ttttggcttc ttttactatt tgatcctcga tttctttttc atgcgggtgg acagcttagt
    71941 tttttcatga gttttttgtt tatcctgatt tcttttgatg gagattggtt gaaaaaaacg
    72001 gcctctcacg tcctgtttac tatgatcacg atgccgttga ttgtatggca tttttatgag
    72061 tggcctattt taggtagtct ttttacatta ctgattgcac cggtattcaa gtttcttttt
    72121 cttcctggtg tgttatttct cacactcttc aatcgctggc tgccggaaac tttacttttg
    72181 ctgatagaag agggattaat cttatttgag cgaataatcg atttttcttc agggtcgtct
    72241 ctcattatag gacgtttgcc ccttgtgcta tctgggatgt atattttcgg attactgatt
    72301 tgtatggata ggttgaaaga acaacctgtg aaacacttgt tttatgttgg tcttttttct
    72361 tttatttttt tgactttgcc atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc
    72421 ggtcaaggag acagtatctt tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat
    72481 actggaggtc agttacactt tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct
    72541 gctgaaagaa atatcgttcc atatctaaaa ggtcagggga taagtgtaat cgataaactc
    72601 atcttgacac acgacgatac ggatcatgtg ggagaattac ccacgttatc tcagcatttt
    72661 actatcaaaa aaatttatat cggttggggc gctggacgaa atgagccatt acaaaggcat
    72721 ttatctgaag ctcaaaaaaa tggtactgag atcattgaaa tcaaacatgg ggacaggatc
    72781 agcggttatt atgatcttta tgtattgact ccttttgaaa agggagaggg aagaaatgaa
    72841 gattcgattg gtctatggat ggaatataac aataggcgct ttttattttt aggagatttg
    72901 aatcaagcaa tggaaaaaca attgcttttg atttatccta atctgaaagc cgatgtagtc
    72961 aaattaggtc atcacggtag ccgtacgtca agtaacacag attttctttc ccatattgaa
    73021 gcaaaacatg ggattatatc ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa
    73081 gtattggaga cactagaaga aaatcagata aaaacatggc gaacggatca acaaggaatg
    73141 atcacctatc gctggcatcc agtttttcat cctcatgggc tagtggaaac aatgatagac
    73201 tagtaattct catctgctca tggtaggata atttttgaaa gtggtgagta atttgagtat
    73261 acaagcagag ttacaaaaaa tacgtaaaga aaaacttgca tcttgttact tgattttagg
    73321 aacagaaaag tttctccaag atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga
    73381 gggcgaagat gacttaaatt ttttaagttt tgacatggaa aatgcaagtt tagatgaggt
    73441 agtagctgaa gcagagacgt tgccgttttt cggagatcaa cggttagtat tcgtagagaa
    73501 tccttatttt ttaacaggtg aaaaagtaaa caatggaatc gaacaaaata ctgatttatt
    73561 gacggcttat ttgaaagaac cattagaatc gactgtatta gtattctttg cgccatatga
    73621 aaagttggat gaacggaaaa aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa
    73681 tgtccaacag ttgaatgaaa aagaagtacg tcaatatctg atgaatacct tagagaattc
    73741 ctcaatcaaa atggaacgaa gtgcaattga tttattcttg aggttgaccg acttagattt
    73801 atccaaaatg atgagagagt tacagaaatt ggtcctttat ggacaaaatc aacagcaaat
    73861 cacggtaaga gaagtagaag aattagttcc taaaacgtta gaacataata tttttgacat
    73921 gacccaatat attttaaaag ggaagacgga acaagctttg cgtttatacg aagatttagt
    73981 tatgcaaggg gaagaaacaa tcaaaattaa tgcgattttg ctttctcaat tacgtttatt
    74041 tttacagaca aaatttcttg taaagattgg ttatcagcag gcaaatattg ctgaaacatt
    74101 gaaaatccat ccatatcgtg taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca
    74161 tttgctggta cagttgtttg atcaattagt tgagatggat tatcaaatca aaacgggaca
    74221 aatcgaaaaa gaattaagtt ttcaactatt cgttttgcgc acagggcagt tgttaagaaa
    74281 tcgataagaa gaaagacgag ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt
    74341 ttgacaaata aaaaaatcgg ccgaagccga ttcttttatt tagcgatttt tttgcttaaa
    74401 cgagatttgt cgcggttagc tttgtttttg tggattagac cttttgattc agccatatca
    74461 atagctttaa cagcttcttt atataacgca tctacgttgt cagcacctga agctacagca
    74521 tcttcaaatt ttttgattgc agtacgcata gcgcttgttt gagatgaatt tttcacatta
    74581 gcgttttcgc ttgtacgaac acgtttgatt gcagattcaa tgtttggcat tagtttcacc
    74641 tccgataact taagttctta cgcgtgttac cgcataattc aacattcatt attataccta
    74701 aaggcagcgt gctttgcaat atgaacatga caagtttttt tccttaacag tcttttatta
    74761 ggattttttt tcagtaaaaa cggatagtac aagctgaaaa aaatcaaaaa gacgctattg
    74821 tgggaggcaa tagcgtcaaa ccaaactaga tttttattta tctatttaaa caaagtttga
    74881 atgggaatgt ttggtactaa gttattatat aacaaagaga accgtttgca aagttttttt
    74941 tgaaaaggga tacgattccc tgttgttctt tgtttttcta ttaagaaact gcagttattt
    75001 tataaccagt atcctttgat cagtaaaggc tattctggtg taccgattat tttaatgtat
    75061 tcatcgatat tttttatcaa catttttttt atgaccaact ggttccgacg gttccatttg
    75121 ccacttactt ccagcacata tttatttagg ggcaaacgta agatatattt tgccaaatag
    75181 cgatctttaa gcaggccatt gatcacacgg tcctcaagct gcaagttgat cccagtcagc
    75241 atcattggct gttcttgtaa aacgtacgta tcaattaaaa gaccttcaaa agtttccaca
    75301 agcgtcttcc tttcttttca aagtaacctg aaaacaggca ctaattcttc cattttgata
    75361 ttcgttgaag tggatgcgat cattctttct tttgtccact gtaatctagt ataacaaaaa
    75421 agagcagaag agccaaaaag aaaaaagagc atcgaattct ataggaagtc tcattggcac
    75481 ggcctaaatt attacaaaac attgattaaa aaaagcccct gcatcgcagg agcatcccct
    75541 atatcgtttt aataggtcaa tcaaaaagca gtatcaaaca cattttgtat caaatcagga
    75601 aataatagat aagctaagcc cagaatcaaa agtaccgcta gaagaaacca gccgattgtt
    75661 cctatgaaat actttaattt ttcgaacatt gtatcacctc gttgtcagta tatcacggat
    75721 cagtaaattg ggggtgttaa gaatctccta aaatagacaa aatttgttct attttcctaa
    75781 agcaaatact tgtaagatac agtaagtgat accagaagca atgaccggtc cggcggcgat
    75841 tcctcttaag aagacaacac ctaaaatcgt gccgaaaaca agtgcaacag tgatttgtgg
    75901 atcagaagca atcaatccta cacccttgga agataagaca gccactagcc caccgcaaaa
    75961 aacagctacc cagccaatag gggatttaaa ggcttctaat agatcacgta gaccgatttc
    76021 tcctgttgcg atagggatta gaatagccgc tgtgatgatc gtcaccccaa tattgattcc
    76081 tttgccttga agccaaggga aaaaggaacg ggaggctggt atgagcttca taatcaaaac
    76141 aaaagctgcc gctatcatca gactttgatt tttagctaaa attgctacaa gtaaaataat
    76201 tcctaaaaaa agccaacttt ccatgaaata tattcctcac tttcttcgaa acaatcataa
    76261 catgttttta gaaagttaca aaagtaggaa ttcatccttc aagacctatt cataagctgt
    76321 ttttttcata aaataaacaa aatcataaaa aaacactttg cttatttgac agaagacagc
    76381 cgcgctttgt atagtggaca agacttacat ttttttgaag aatcagaaag atcaggtaaa
    76441 ggtcttagta aacgcaagcc attcagaata accaatattg tgcttccttc gtgtcctaca
    76501 actccaagag gtagatttaa aaattgcagg aagttagaag caatcaacaa gagaataaca
    76561 agaatcgaaa aaataatatt ttgtttgatg atccgccgca tttttaaaga tagccgatga
    76621 ctgtagacaa atttatttaa gtcgcttttc attaagacga tgtctgcgat atccatagcg
    76681 gcatctgtcc cttgccccat agcgacacct aaagaagcgg ctgctaaagc aggcgcatca
    76741 ttgactccat caccaaccat cgcatttact tggtaaagtt cattctcttt tttgactagt
    76801 tgtgtttttt cttccggagt acagcttgca tagtaatgtt caatccctac ttgttcagca
    76861 atggcctttg ctgtgccaga atggtcgccg gttatcatac ttgtatggat gttttgggat
    76921 ttaaagtagg aaattacttg ttgggtattt gcttttggaa tatctagcag acctaaaaca
    76981 gcaattaact gctgatcttt agaaagataa atcacggttt tgccttgatt ttccaaacgt
    77041 tcgatcattt ccgcaatctc tttgctgatt atcatcgtct caggatcata tgcgtgcttg
    77101 ccaactttcc attgcgcacc taaataggta gtttgtaatc caaaaccagt aatttcttct
    77161 attggtaagt attggatttc ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga
    77221 gcaagtggat gcgtcgtttt ttgttccata gcaacaagaa gttgttgtgc caaatccttt
    77281 tcatcaaaga aaaagctgtc agttacaact ggttttcctt tagtgagcgt tcctgtttta
    77341 tcaaaggcaa tcgcttttaa ttcagaaagc ttttctaaat ggataccgcc tttgattaaa
    77401 atcccatttt ttgcagaatt tgaaagagca gctagagaag ctggtgtagc agatgcgaca
    77461 agcgcacaag gagaagccac aactaataat accattcctc gataaaagct ttcatcccaa
    77521 ctccaaccta ggaaaaagtg ggggagaagg atcattagag gaacagcaag caaaatcaat
    77581 tttacataat tattttcgat tcgttcgata aatgaagctg tttgagttgg cgtattctgc
    77641 gcttcttcta ctaattggat aatcttagca aagacagttt cggaactcgt ttttgttact
    77701 cgaatcgtga tgggatttcc ttgattcagc gttcctgcat acatctcatc gcctaaatat
    77761 ttttctacag gcacagattc accgcttaat gcagactcat cgatcgttgc ctgtgtgctt
    77821 tctaaaaagc catctatcgg aacagctgct cctttagcga caaataccaa gttatccacc
    77881 tttagttgag aaacatctac ttcttgcatt tgtccatttt cttttaataa ttgggctttt
    77941 tgtggttgaa gattcatcaa agcagtaatt tctttttgac ttttattagt tgtgtattcc
    78001 tctaatgcac cgcttaaaca aaagatgaat gtcaacattg ctccttcaaa gtaattaccg
    78061 atcaaacagg caccaatagc agctaaagcc atcaataaat ccacattcag tgtccgatca
    78121 ttccacaatt ctttcaagcc ttccgatgtt tgtttatatc caccgatgat aatggcactg
    78181 atataaaaaa gtggggtgat ggtaagagag aatcggtcga agataaaacc gaagatcata
    78241 aataaaaggc agccgattgt tgctgcaaaa gcttttttct gattagtcat cgttattaga
    78301 cctccttgtc aaaacaagga tagcataata aaaatgtaat tgataataat aacagctaaa
    78361 tgagaacgat aatggttatc atttggtgat taagcttgat gttgttttta ttatttataa
    78421 tgattattat taaagaaaat aaaaaaccag aacatttctc aaataagaga agtgttctgg
    78481 ttttttctac attgcaataa accaataacc aatcaaaatg ataccaagga taatacgata
    78541 ccagccaaaa gcggtaaagt cgtttttctt caaatagctt agtaagaatt tgatagctaa
    78601 gacggaaacc acaaaagata cgaatgttcc tacaagaaga acaacagttt cttccatttt
    78661 gaatgaattc ccttcagcta aatatttgac gatcttcaag ccgcttgcgc caaacatgac
    78721 aggaatccct aagaaaaatg aaaactctgt agcgacaaaa cgcgaagcac caatcaagat
    78781 agatcctagg atcgttgcgc cagaacggga tgttccggga acaagtgcca agacttggaa
    78841 taacccaaca agaagagctg cttggtacgt aaagtcattc agagaagtcc aacgaggtgt
    78901 tttgtttttg ttgcgttttt cgacaataat aaaggcaata ccgtaaacga tcaaaacaat
    78961 ggcaactggt aagaacttgt ggaaatttgc ttctaaccaa tcatccaaag gcaaaccaat
    79021 gatgatggct ggtatgcaag caacgatcac tttaaaccaa agtgtccacg tatctttttt
    79081 ttctaccgtt gatttacttg gagaaaatgg gtttaacttg tgaaaataaa gaacgacgac
    79141 agccaagatc gcccctaatt ggataacgac attgaacatc gacataaatt gtgaactggc
    79201 atccagtttg atgaattcat ctgctaggat caaatgacca gtactactga tgggcagcca
    79261 ttccgttatt ccttcaataa taccgagaat cactgctttg ataatatttg caaaaaacat
    79321 aaattccttc cttcccaatt aagatacatc ttatagtata ccgttatttt ttgagaaacc
    79381 caatctgttt tttaatatta agaaaaactt gagaaataaa gcagataagt acacgaagag
    79441 aaaaaagaag acaaagccct cgttcagact ttgcctttta tgattctctg gtaaaaatta
    79501 aaatatcccc attatagaaa cgagtcgcga ctatgtgttt tcccgttatc gatataagac
    79561 gtatcattga tgccgtggat agaaaatcga ccgtttgcat aagtgacctt tgttacgctg
    79621 ccatttaaaa gcccagcacg aacaggctgt ttaggatcga tcagattaag caaaaaggcg
    79681 atcgtcaaac catgagatac gactaaaaca tttccaccac catttttttc gcagtgatgg
    79741 gcgatatctt caaatcctgt ccatacacgg tctcgcaatg tttcataagt ttccgcccac
    79801 ccagcggtat ctaagttgta tacagcatta gcaatttgtt caaaagaaac ttctgtggta
    79861 aacatttcat catatgtttt aaaattcaat acacgaggga tcacacccca catttccatg
    79921 tcatatccac cttcaagaga cccgaaacac cattctctaa tccttttttc aataaaataa
    79981 ggaatttctt ttcccttaga atgttctcct agaataatac gggcagtttc gattgcacga
    80041 ccactgtcac tagaataagc aaatgaaaag tcgacatttt ttaacccgcg acctaaataa
    80101 tgaatgcctt cttgtccttg ctttgtgagg ggtgtatcac accagccttg tactcgttca
    80161 attgtattga acatcgtttt tccgtgtcgt acgatataaa gtgtagtttc tgtcatttta
    80221 ttctcctcct aagtcgtttt aacgattgaa aaaaggattt gtcctttttt cctgttcaat
    80281 cgtcgttgca tcgccatgtc ctggatagac tgcaaattca tcaggtaata caaacagctg
    80341 ggtccgaata ctatgaagta attgttctaa attccctgta tacagatcgg ttctaccgat
    80401 acttcctcta aacagggcgt cgccagaaac gacaaaatca tcaaaaataa agctgacgct
    80461 tcctatagaa tgccctgggg tagggacgac gcgaaaagac atgtttccta aagtataagt
    80521 tttcatttca aattcatatt ctgcaggcga gacaataaca tcagcaatat catcatgtct
    80581 accgagtcca gaaagattca aaatcggatc acctagccat ttttgctcta gtgggctgac
    80641 ataaactggg atttgataat acgcgcgtaa aggttccact gcaccgatat gatcgtagtg
    80701 agtatgggta agcaaaatcg ctaccggctg ttggtttgtt tttttgattt cttgtttgat
    80761 tcgttccgca tcttctccag ggtcaatgat caaaagtgct tcttcgttgt agaccaaata
    80821 acagttttct tcaatcattc ctgtttttaa tctttcgata tgcatgtgca ttcctccttg
    80881 atagccaaaa cttctttcat ccattagtat aacgcattca ttgctaggaa acaaaaacag
    80941 gtacttattt ctctttactt atctgaatta tcagactata tgaaaagtat ttggatattc
    81001 taaagagaag gaaagtaagt tttcatagca ttttaatatg tccttgttac attagattca
    81061 tccaaaaatc caaattctat atcctatatc cctgcctaat cagcagggtg tttttttatg
    81121 tttggaaaga gagctctttt tgctaaacta ggttttttca ggtaatctta gagagaaaga
    81181 ggtggtggaa atgatcaaac agtcaaaaat atctgtgcgg cacttagtag aatttatttt
    81241 gcgacgtgga agtatcgaca atcgtaaaaa aagtaaccat actgcattag aaggagcgag
    81301 aatccaccgc aagctccaaa aagatgcggg agaaaactat gaaaaagaag tctttttaaa
    81361 aacaacagtt caattagatg aatattacct gatcgttgaa ggccgtgcag acggtatttt
    81421 caaaagagac ggtatttatt atatagatga aatcaaaacc tccgaaccac gatttgaaga
    81481 tttagaacca gaacaagtgg aacttttctt tcatcaagca agggtgtatg cctatatcta
    81541 tagtcaagaa catgatttac aagaaataaa tctgcaattg acttacttcc agacggcaga
    81601 agaagtgatc acgagaaagg ttgaacatca gacaagagag cagttagaaa ctttttttaa
    81661 aaagctgact gaagattata aagaatggct tgtttttcaa gaaaactggc gcactgtacg
    81721 caatgcctct ttgatggctt tgaaattccc ccatgatgag tataggaaag gtcagcgtga
    81781 actagcggta gctgcttata aaacgatccg tacgaaacag aaattgtttg tagaagcacc
    81841 taccggaaca ggaaaaacaa tctctacgtt attccccgct ttgaaagctg taggggaaga
    81901 agagggagaa aagatctttt atctaacggc taaaacaata actagacaag tagctgaaga
    81961 tgctatgaca gcattaaaag atacaggagc tgaagtaaaa agtgtcactt tgacggcgaa
    82021 agacaagatc tgctttttag atgagacgac ctgtaatcca gatcagtgcc catatgccaa
    82081 tggttattac aatcggatta atgaaggttt atgggatctt ttgaatcatg agaatcagat
    82141 cactcgtgaa gtcatcgaaa cttatgcaag aaagcataca ctttgtcctt tcgaactatc
    82201 attagatgtc agtatctggt gtgacgtaat cattggggat tacaattatt tatttgatcc
    82261 aactgtttat ttaagacgtt tttttgaaga tgaaaaaaac gaggactatc tgtttttgat
    82321 tgacgaagca cataatttgg tgaatcgttc ccgagagatg tattcggcag aactatctta
    82381 tgaaaaaacg aaacgaagta aggaagctat accaaaggaa ttcaaaaaac ttcatcgccg
    82441 tttcaacaaa ctattaaaag aatttgattc gatccgagaa atagcaaaag aagatcattg
    82501 ggattatcat catcaaaaag caccggctga atctttagta aaggcaggtt atcaattaag
    82561 cgaaaaaatt aaagaatggc tggcagaatt tcctgagcac ccatcacaag aacaattgct
    82621 tccttattat tttgatttgc ttcatttttt gaaggtcagt gaattttatg atgatcacta
    82681 cgaaacaaca gtcgaaaaaa cgtatcgcga tctgatagta aaagagtttt gtattgatcc
    82741 aagcttgttt cttgagcagt cgttggataa aggaaaaagc agtattcttt tttctgctag
    82801 tttttcacca ctctcttatt atcaggaaac actgggggga caaaaaagtc tggcttacaa
    82861 actgccgagt cctttcccag aaggaaacca gcaagttttc atcgcaaatt acctagagac
    82921 gacttatcga aaaagggaac acagtctggc aaaattagta gagacgattc atgcttttgc
    82981 tgatggaaag gtcgggaatt atttcgtttt ctttccttct tatcaatatt tagatcttgc
    83041 ggtggaagca ttcagacagc aatatcccga cagcaatgtg ctaatccaag aaacggacat
    83101 gaatgaggaa gaacgcgaac gatttttaac aaaatttcaa acaaatcccg ctgaaacatt
    83161 gattggattt tgcgtacttg gcgggatttt ttcagaagga atcgatttga gaggggaccg
    83221 attgatcggc agtatgatcg taggtgtagg tttgccgcag atgaatcatg agcaggaact
    83281 gatcaaatca tattatgatg agaaggaaca ccttggattt gcttacgctt atcagcttcc
    83341 tggaatgaac aaagtcttgc aagctggtgg tcgagtgatt cgtggcatgg cggatcaagg
    83401 aatcgttgta ttggcagatc aacgattttc tagtttttct tatcgacgtt tattcccaca
    83461 acattggcaa acagcaagag aagtacgaag tgttggggaa ttggaagaag gaatacagcg
    83521 tttttggtta tcaaaagaag cagtaaatca aaaaaacgag gaatgacaga cgtcagcctc
    83581 gttttttgat taacgatagt tagtgaattg caactcgatt ggtaagtcaa gtttacgtaa
    83641 taaagcaatc acagcttgta gatcatcacg gttcttacct gtcactctaa tctgatcttc
    83701 ttggatttgt gcttttactt tcagtttttc atttttgata gctgtagtga ttttcttcgc
    83761 gttttcacga tcgattccgt tgaccaaatc ggcagtttgt cgagctttgc cacctagagc
    83821 gtgttctgat tcggaaaaat ggatattttt gattggcaca tttcttttga tcagcttacc
    83881 aaataataca tctttgatct gttcgatttt gaaatcatca tcgctcaata caactagatt
    83941 gctgttctct aatttgattt ctactgtaga atccttgaag tcaaagcgat tggacaattc
    84001 ttttgttgtt tgctgaatcg cattttttac ttcttctgta ttgacttctg atacaatgtc
    84061 gaagctagca tcttttgcca taggaaccct cctgatatac atataaatat ttaccataca
    84121 tctatcttat cataggatta gggcagattc ttacctgttt gctaaaattc ttctcatttg
    84181 agttcgaagt attttatttt aatagataaa ctgttattct tacatttgga ataccaaagt
    84241 aggggagagg tgcttgtgag taaaattatg actgcgtatc ataaaaatga aagattaaag
    84301 aaaatagtgg ttattttgat aaccggattg accgctgcgg tcgctttaaa ttattttttg
    84361 attccagcca atgtcttttc tgcagggatg aatgggatcg ctcaaatcat ttcctcgctt
    84421 ttatatgaat ggtttcatat caagatagac acaggaacat ttatttttct tttgaatatc
    84481 cctgtgtttg tattagggtt tctcaaagta ggaaaacgag caacgatcct tagttttgtg
    84541 aatgttgtat gtgtatcggt gatgacgatg ctgcttccag tcggacagat cactgataat
    84601 attttgatga atgccctagt aggcggtgtt ttattaggtc ttggcgtagg attatcttta
    84661 aaaatgggct ttacaactgg tgggatggat atcatctctc ttgtactttc acaaacaact
    84721 ggaaaaacag tcggaaacta catgctgatt ctaaatggta tcattgtagt catcgcaggc
    84781 ttcttcttta actgggaaag tgccttgtat acgattattt ccatttatgc aatgactcaa
    84841 gtaattgatg ccatccacac gagtcatcaa aaagtaacag caatgatcgt taccgtcaag
    84901 ccagaagagg taacagaaga actagcaaaa cggatggtac gtggaatgac attactgccg
    84961 tctgtcggtg gctattcaaa aaaacaaggg aacatgatca tgatggtcat cacccgctac
    85021 gaaatgtatg atcttgatca gatcgttcat gaaatcgacg acgatgcatt tatcaatatt
    85081 ttaccaactc attcagtatt tggccggttt gcaaatgaaa acgaacaaaa aatgtatcga
    85141 acaactggcg tattgccaga aataaaaaat cgttcacaaa aaaaatgagg aatcaggttt
    85201 attttagaat aaatttaatc tattacatct aataagaaca tttttataaa tttaaacgag
    85261 aatcttactt ctaaattctc atttacaagc agctaaaaaa agagtatgat aaataaaaat
    85321 cgtgttaagg agtgtcgtac gatgagcgaa gcaaaccaag aacggtctgg acaagaaata
    85381 gaactaatca aagaacgtat tcttgctgcc ttggaaatgg tgattgatcc tgaattagga
    85441 atcgacatcg tcaatttagg cttgatttat gatatagaat tcaatcctga aaatggagaa
    85501 acagtcatta agatgactct aacaactatg ggctgtccat tagcagatat tttaacagaa
    85561 tctatccatg acgcattaaa agaagtgcca gaagtaacga aagcagaagt caaactagtc
    85621 tggtatccag catggacgac agataagatg agccgttatg cacggattgc gttggggata
    85681 cgatagaaat cttggctgac tagctatgag aagcaaatag gaaaaacaaa aaagcctttt
    85741 atcctttcta gaagaaaaga gataaaaggt ttctttgttt ataaaa
//
LOCUS       DANKBF010000003        80550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000003 DANKBF010000000
VERSION     DANKBF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            121..1440
                     /locus_tag="PWY89_000214"
     CDS             121..1440
                     /locus_tag="PWY89_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HDL2065282.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1629..2030
                     /locus_tag="PWY89_000215"
                     /pseudo
     CDS             1629..2030
                     /locus_tag="PWY89_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            complement(2335..2649)
                     /locus_tag="PWY89_000216"
                     /pseudo
     CDS             complement(2335..2649)
                     /locus_tag="PWY89_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(2903..4538)
                     /locus_tag="PWY89_000217"
                     /pseudo
     CDS             complement(2903..4538)
                     /locus_tag="PWY89_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4861..5340)
                     /gene="rlmH"
                     /locus_tag="PWY89_000218"
     CDS             complement(4861..5340)
                     /gene="rlmH"
                     /locus_tag="PWY89_000218"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2065283.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            5768..7057
                     /locus_tag="PWY89_000219"
     CDS             5768..7057
                     /locus_tag="PWY89_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL2065284.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(7096..7701)
                     /locus_tag="PWY89_000220"
     CDS             complement(7096..7701)
                     /locus_tag="PWY89_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL2065285.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(7906..8370)
                     /locus_tag="PWY89_000221"
     CDS             complement(7906..8370)
                     /locus_tag="PWY89_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2065286.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(8392..8712)
                     /locus_tag="PWY89_000222"
     CDS             complement(8392..8712)
                     /locus_tag="PWY89_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2065287.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(8856..9935)
                     /gene="pepA"
                     /locus_tag="PWY89_000223"
     CDS             complement(8856..9935)
                     /gene="pepA"
                     /locus_tag="PWY89_000223"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL2065288.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            10135..10485
                     /locus_tag="PWY89_000224"
     CDS             10135..10485
                     /locus_tag="PWY89_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2065289.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10708..11655
                     /locus_tag="PWY89_000225"
     CDS             10708..11655
                     /locus_tag="PWY89_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2065290.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(11712..13130)
                     /gene="pepV"
                     /locus_tag="PWY89_000226"
     CDS             complement(11712..13130)
                     /gene="pepV"
                     /locus_tag="PWY89_000226"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL2065291.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            13279..14124
                     /locus_tag="PWY89_000227"
     CDS             13279..14124
                     /locus_tag="PWY89_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL2065292.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            14128..14568
                     /locus_tag="PWY89_000228"
     CDS             14128..14568
                     /locus_tag="PWY89_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL2065293.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            14618..14812
                     /locus_tag="PWY89_000229"
     CDS             14618..14812
                     /locus_tag="PWY89_000229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065294.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            14948..15634
                     /locus_tag="PWY89_000230"
     CDS             14948..15634
                     /locus_tag="PWY89_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2065295.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(16042..16731)
                     /locus_tag="PWY89_000231"
     CDS             complement(16042..16731)
                     /locus_tag="PWY89_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065296.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(16706..18835)
                     /locus_tag="PWY89_000232"
     CDS             complement(16706..18835)
                     /locus_tag="PWY89_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL2065297.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(18883..20220)
                     /locus_tag="PWY89_000233"
     CDS             complement(18883..20220)
                     /locus_tag="PWY89_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2065298.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(20472..20831)
                     /gene="rplT"
                     /locus_tag="PWY89_000234"
     CDS             complement(20472..20831)
                     /gene="rplT"
                     /locus_tag="PWY89_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2065299.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(20884..21084)
                     /gene="rpmI"
                     /locus_tag="PWY89_000235"
     CDS             complement(20884..21084)
                     /gene="rpmI"
                     /locus_tag="PWY89_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2065300.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(21156..21680)
                     /gene="infC"
                     /locus_tag="PWY89_000236"
     CDS             complement(21156..21680)
                     /gene="infC"
                     /locus_tag="PWY89_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2065301.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(21724..21849)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            22000..22632
                     /locus_tag="PWY89_000237"
     CDS             22000..22632
                     /locus_tag="PWY89_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2065302.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(22634..23539)
                     /locus_tag="PWY89_000238"
     CDS             complement(22634..23539)
                     /locus_tag="PWY89_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2065303.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(23624..24283)
                     /locus_tag="PWY89_000239"
     CDS             complement(23624..24283)
                     /locus_tag="PWY89_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2065304.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24599..25543
                     /gene="mvk"
                     /locus_tag="PWY89_000240"
     CDS             24599..25543
                     /gene="mvk"
                     /locus_tag="PWY89_000240"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2065305.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            25536..26513
                     /gene="mvaD"
                     /locus_tag="PWY89_000241"
     CDS             25536..26513
                     /gene="mvaD"
                     /locus_tag="PWY89_000241"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL2065306.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            26526..27611
                     /locus_tag="PWY89_000242"
     CDS             26526..27611
                     /locus_tag="PWY89_000242"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL2065307.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            27608..28672
                     /locus_tag="PWY89_000243"
     CDS             27608..28672
                     /locus_tag="PWY89_000243"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL2065308.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(28921..29724)
                     /locus_tag="PWY89_000244"
     CDS             complement(28921..29724)
                     /locus_tag="PWY89_000244"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL2065309.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(29744..30586)
                     /gene="kduI"
                     /locus_tag="PWY89_000245"
     CDS             complement(29744..30586)
                     /gene="kduI"
                     /locus_tag="PWY89_000245"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL2065310.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(30602..31609)
                     /locus_tag="PWY89_000246"
     CDS             complement(30602..31609)
                     /locus_tag="PWY89_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2065311.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(31616..32260)
                     /locus_tag="PWY89_000247"
     CDS             complement(31616..32260)
                     /locus_tag="PWY89_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2065312.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(32360..33118)
                     /locus_tag="PWY89_000248"
     CDS             complement(32360..33118)
                     /locus_tag="PWY89_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2065313.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(33245..34396)
                     /gene="fucO"
                     /locus_tag="PWY89_000249"
     CDS             complement(33245..34396)
                     /gene="fucO"
                     /locus_tag="PWY89_000249"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL2065314.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(34478..34789)
                     /gene="rhaM"
                     /locus_tag="PWY89_000250"
     CDS             complement(34478..34789)
                     /gene="rhaM"
                     /locus_tag="PWY89_000250"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL2065315.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(34791..35636)
                     /gene="rhaD"
                     /locus_tag="PWY89_000251"
     CDS             complement(34791..35636)
                     /gene="rhaD"
                     /locus_tag="PWY89_000251"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL2065316.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(35650..36906)
                     /gene="rhaA"
                     /locus_tag="PWY89_000252"
     CDS             complement(35650..36906)
                     /gene="rhaA"
                     /locus_tag="PWY89_000252"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL2065317.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(36903..38363)
                     /gene="rhaB"
                     /locus_tag="PWY89_000253"
     CDS             complement(36903..38363)
                     /gene="rhaB"
                     /locus_tag="PWY89_000253"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL2065318.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            38516..39385
                     /locus_tag="PWY89_000254"
     CDS             38516..39385
                     /locus_tag="PWY89_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL2065319.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(39511..40410)
                     /locus_tag="PWY89_000255"
     CDS             complement(39511..40410)
                     /locus_tag="PWY89_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065320.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(40575..41399)
                     /locus_tag="PWY89_000256"
     CDS             complement(40575..41399)
                     /locus_tag="PWY89_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2065321.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(41410..42705)
                     /locus_tag="PWY89_000257"
     CDS             complement(41410..42705)
                     /locus_tag="PWY89_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL2065322.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(42721..43200)
                     /locus_tag="PWY89_000258"
     CDS             complement(42721..43200)
                     /locus_tag="PWY89_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2065323.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(43206..44234)
                     /locus_tag="PWY89_000259"
     CDS             complement(43206..44234)
                     /locus_tag="PWY89_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2065324.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(44251..45357)
                     /locus_tag="PWY89_000260"
     CDS             complement(44251..45357)
                     /locus_tag="PWY89_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2065325.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(45586..48183)
                     /gene="adhE"
                     /locus_tag="PWY89_000261"
                     /gene_synonym="adhC"
     CDS             complement(45586..48183)
                     /gene="adhE"
                     /locus_tag="PWY89_000261"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2065326.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(48488..48868)
                     /gene="yajC"
                     /locus_tag="PWY89_000262"
     CDS             complement(48488..48868)
                     /gene="yajC"
                     /locus_tag="PWY89_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2065327.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(48965..50110)
                     /gene="tgt"
                     /locus_tag="PWY89_000263"
     CDS             complement(48965..50110)
                     /gene="tgt"
                     /locus_tag="PWY89_000263"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2065328.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            50328..51026
                     /locus_tag="PWY89_000264"
     CDS             50328..51026
                     /locus_tag="PWY89_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2065329.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(51194..53833)
                     /locus_tag="PWY89_000265"
     CDS             complement(51194..53833)
                     /locus_tag="PWY89_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2065330.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(54452..55483)
                     /gene="queA"
                     /locus_tag="PWY89_000266"
     CDS             complement(54452..55483)
                     /gene="queA"
                     /locus_tag="PWY89_000266"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2065331.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            55676..56850
                     /locus_tag="PWY89_000267"
                     /pseudo
     CDS             55676..56850
                     /locus_tag="PWY89_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            56854..57495
                     /locus_tag="PWY89_000268"
     CDS             56854..57495
                     /locus_tag="PWY89_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065332.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            57492..58409
                     /locus_tag="PWY89_000269"
     CDS             57492..58409
                     /locus_tag="PWY89_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2065333.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            58406..59077
                     /locus_tag="PWY89_000270"
     CDS             58406..59077
                     /locus_tag="PWY89_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065334.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(59618..60106)
                     /locus_tag="PWY89_000271"
     CDS             complement(59618..60106)
                     /locus_tag="PWY89_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL2065335.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(60307..60573)
                     /locus_tag="PWY89_000272"
     CDS             complement(60307..60573)
                     /locus_tag="PWY89_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2065336.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(60573..60836)
                     /locus_tag="PWY89_000273"
     CDS             complement(60573..60836)
                     /locus_tag="PWY89_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2065337.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            61312..61497
                     /locus_tag="PWY89_000274"
     CDS             61312..61497
                     /locus_tag="PWY89_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2065338.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(61639..63462)
                     /locus_tag="PWY89_000275"
     CDS             complement(61639..63462)
                     /locus_tag="PWY89_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL2065339.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(64060..66765)
                     /locus_tag="PWY89_000276"
     CDS             complement(64060..66765)
                     /locus_tag="PWY89_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL2065340.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(67071..68018)
                     /locus_tag="PWY89_000277"
     CDS             complement(67071..68018)
                     /locus_tag="PWY89_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL2065341.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            68208..68477
                     /gene="rpsN"
                     /locus_tag="PWY89_000278"
     CDS             68208..68477
                     /gene="rpsN"
                     /locus_tag="PWY89_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2065342.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(68653..69015)
                     /locus_tag="PWY89_000279"
     CDS             complement(68653..69015)
                     /locus_tag="PWY89_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2065343.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(69026..70147)
                     /locus_tag="PWY89_000280"
     CDS             complement(69026..70147)
                     /locus_tag="PWY89_000280"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2065344.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(70163..70513)
                     /locus_tag="PWY89_000281"
     CDS             complement(70163..70513)
                     /locus_tag="PWY89_000281"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL2065345.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(70643..72154)
                     /locus_tag="PWY89_000282"
     CDS             complement(70643..72154)
                     /locus_tag="PWY89_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2065346.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(72476..73828)
                     /locus_tag="PWY89_000283"
     CDS             complement(72476..73828)
                     /locus_tag="PWY89_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2065347.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(73981..75057)
                     /locus_tag="PWY89_000284"
     CDS             complement(73981..75057)
                     /locus_tag="PWY89_000284"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2065348.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            75253..76998
                     /locus_tag="PWY89_000285"
     CDS             75253..76998
                     /locus_tag="PWY89_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL2065349.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(77278..77715)
                     /locus_tag="PWY89_000286"
     CDS             complement(77278..77715)
                     /locus_tag="PWY89_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL2065350.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(77699..79072)
                     /locus_tag="PWY89_000287"
     CDS             complement(77699..79072)
                     /locus_tag="PWY89_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065351.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(79394..79882)
                     /locus_tag="PWY89_000288"
     CDS             complement(79394..79882)
                     /locus_tag="PWY89_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2065352.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(79904..80506)
                     /locus_tag="PWY89_000289"
     CDS             complement(79904..80506)
                     /locus_tag="PWY89_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2065353.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        23291 a        17093 c        14668 g        25498 t
ORIGIN      
        1 aaactgcctc acggcagctt acaattactt tttcatcttc acccgttaag tgatgaaaaa
       61 agaacccctt tctgctatgg ttaaagtgac taaaccaacc aaaagaaagg agttcttttc
      121 atgactagct tacacaaaaa ccaagtaaaa ttcaattcaa atatcactat ttctcataca
      181 ggtggtcaat tatcgagtga ttcgggtctc gtcctagtga aagagctgat gaacatcttt
      241 ggtttttctg aactggctaa gcaacacatt cacattgaag acgaacgagc gtattttact
      301 catgacaact tatccatact tgagcagttc attatgcaat taattgctgg ttactcagct
      361 gattctgcag ctaatctcct gagacaagat cctgtgttta aagctgtttt agataggaaa
      421 gaactcgctt ctcaatcttc actttcccga tttttagatc gactatctga ggagaatatc
      481 catgaacttc aagctttgaa ccaagaactt atcgataaag cacgcctcat ccgtaatgat
      541 acggaattaa tcattgattt agattcgacc cattcagata catttggtca ccaagaacaa
      601 acgaattata atacacacta ccaaacctat ggttaccatc cattggtagc ttttgacgga
      661 ttgactggtg acttcttaaa agctgaacta cgttcaggaa atcaatatac atcaaaaggc
      721 gtaaaggagt ttctcacacc tttattagag cactataatc actctctacc aaacactgac
      781 atcttggttc gtggagacag cgggttcgct acacctggtg tgtatgattc gtgtgaatca
      841 aaaaagagtc attatgttat tcgactgaag aataatcgta gactaggtca aatagctgag
      901 aaatcagtac tttatggcga taatcaaaag tgggaagaac gagaagttca gtacttctcg
      961 accacttatc aagcacaatc ctggtcacaa agtcgtcgcg tgtgtatacg ctcaacacgt
     1021 gaagcgggcg aactactctt tcgacatgaa tttatcgtga cgaatctatc agaaaatgtt
     1081 tctcctgata ccatcttttc tatctatgcc aaacgtggca caatggagaa cttcattaaa
     1141 gaagcgaaag ctggctttta ctttgacaaa acagacagtc ctcgcttttt ggagaatcat
     1201 gtccgaatga tgctcagctt gatagcttac aacttagtca acttcttaag aacgattggc
     1261 tttgaagaag tccaaaaggg aatgaccatt cattctattc gattgaagtt tctgaaagtt
     1321 gctgggaaat tagtccaaac gggtagacga gtctatctca aattatctag ttatcatgtg
     1381 tatcagaatg aattctacag ggtctttgct cgcctgaggc gagccagtca atggatctaa
     1441 tccaacaatc taacgatttt tttactggga agttatccag ggagaagtat gctcaaaatg
     1501 ccttacaccc aaaatttatt cctcaatatt tttgaacagt gaatgtttga agtaactttt
     1561 tagtcaaaat cagttgatag ggatattagt gatacatatt tcaataaaat gtgccaaaga
     1621 attaaagcta tgaattattc agggatcaat tagaaagcaa cttttttcgg gatctaaatg
     1681 aaattctctc ttatgatcca gcccatagct atgaggaact atacactcgc ttgattgact
     1741 atgtttacgc aaatactttt ttatatcgct tatttatcct caaccatggt gatatttcgt
     1801 ttcagaagaa agttaaacaa gttattgaag aaaactatct gaacatatgg ctatatgaag
     1861 acaagaaaac gattatcaca gaagaaatga gattctttac tacgtaccac gtccaaggtt
     1921 gtctatccgt tctcaatcgg tgggtagaaa gcaattacaa gtactccaaa aaagaattat
     1981 tagaactctt acagaaactg aatgatcaca tagagaaaat catgccttga ataaaaacat
     2041 actaagctta gttaagaaga accgtaacct tctcgtataa caactgaatc ttatttagcc
     2101 aaattgttta ctgaagattt tttgagtaaa atttctataa ttcctactta ttgaaaagta
     2161 gtttgtagtg ctgcttctcc ctctctaagt gatatatctt gttgtaccaa tcaaattcac
     2221 ctcatcatta ataaattact tttaggctaa tttattacaa cttataggtt gaagctgtaa
     2281 ccaatttata gtcatattat atttttatca tccagtttat tgaattcttc tcgtttacct
     2341 caattcattt tcaaggggaa tcagctcatc atctaacatt ttacgccatg ctcgcttcat
     2401 ttcttgtggg tatttaaatg gtttcgcttc tggatgtttg gctttatatc cttcgtaatc
     2461 cttactgttg ataattgctt tcatattagg aattggatct tcacctggca tatattgcag
     2521 attagaagca tgtaattttt tctcagaaac aaaccactca ttggaaaaga catgaatcaa
     2581 atcatcactt acttgtgtaa aatagcgacg tttaatgaca aagatatctt cattgctatc
     2641 tagtgtacct tcatttcttt gtgcttaaat tcaaagataa ttttctcaat aaagattttt
     2701 gattccagta agattccttt actcctttta caacatagcg ttgtcctttg ttttgaaaca
     2761 tcttcttccc ctctttgcca ttaaaaatag cccaattctc tataaagaag attaagcttc
     2821 attattataa tataaagttt aaatttggtt tattacttac tttttcttat tctctagtaa
     2881 gctaatattg aaaaaaatgt aatcagatca ttatttttat atgttgtaac ttcctgataa
     2941 gaaaactaat taaaagaaag aggtaactac ataaactaat taaaaaagct aacttcatag
     3001 ttggaggata aatggaagaa acttcaatga ttgtttctcc ttgtgttcct tcaatcgaca
     3061 ctagtcctat tatcaatctc tcttttttgt ccatttactt ttgcgactgt tccccaataa
     3121 tcaaatacag gaagcgttat tttttgtcct ttagattcat gtactgtata gactattttc
     3181 gaatccatca cttgataagg catagtatgc cattccttgc caattcttac ttcatgattc
     3241 tgcaaatgtt ctttgtattg taatgcttcc gtgggtgcat agtcataaag tcctccggga
     3301 aggagtgtcg tgtaacgaat aagattttct tgattcatta ccaagtgatc tctctgagtg
     3361 acaactttat gttcatagtt tgcttttgtt gcagcagata cagccaacag taataaaaca
     3421 attgccgtta tattaactac ttttctttgc ttagctgatg tgctcttcat gaaaacgaac
     3481 gataaaatca acgagccaaa cagtacttga aagcctaaga ttcgccaagg aaattgaatc
     3541 atttgaatgg gtgtattttg taataaagac catggaaaaa atttggtgga taagagccaa
     3601 gagactaccg cactaaggta aatcactttt tctttgcgat taaatccttt aaaaaagaag
     3661 agtggacaga tcaaagaaat aataccaatg attcctaccg taaaggttgt caggtcatta
     3721 ttcatcgcac caatcaaact atccgagaca tttaatgctt ggtgttgtaa ggtcgtttta
     3781 tctggctcat taatcgattg aaccatgatt tgtcggaaca tcggatacca aaagtatgct
     3841 gttaaaagaa tcgtcattcc tactgccttt gacaactcca caatcaaccg gatgctttct
     3901 ttttttcttg tttcacttac tacaaagccg cctaagacta gtaagccaac ggctattaca
     3961 ctcataaaag tggaaagaac atgtgtataa atgagtaagc tcatactgac tgctaatgga
     4021 attgcacatg gctttttctc ataaatcagt gcgtaagcat aatacataac tatcggtata
     4081 accgtaagtg caatcgcttc agcaatagca gaacgatgat aaatatcaat cgtacggtac
     4141 ccataaaatg tatataaaca gctaaatata aaagccgcat cgattctttt taaaaatttt
     4201 agtccataat gaaacatcag catgatagta caaacattca atagccaaac atataagata
     4261 taactaacga ttaaattatt acttatccaa taaaaaatga ctgccggaaa aaaggtcaag
     4321 tagggataaa aataattcac tccgctccca taatgattaa acgtcgtaaa attgattgga
     4381 ccagaaaaaa ttgaagataa ccctttgact ctagcaaaat ggaaggcaat atcccccgat
     4441 gtagaaatag tatgaacatt tctaaaataa ttgattgaat aaagtccagc aataattgct
     4501 aggagaatgg acaatattat ttttttcttt acttgcatta cttaattata tactcctttc
     4561 atggattgct ttatctagta taacttaaat tttaaataaa tcgttattta tttttactct
     4621 cacttaaaaa agcactaaaa ttataagaga gggaggtaat ggtctgaaca ccaagaattg
     4681 ttccaacatc gaattgctta actaatgagt tcaaagctta attatgtggc tcttatattt
     4741 accgagcgtg gattgcgatc tctttagcga aacaaacatc tttcataaaa aaaacagcca
     4801 tatccgcttt cttggatatg actatttact actactttac tatctttcat tcttcttgtc
     4861 tcacttatga taaggcgccc ccgcattgat ccggaaggca cggtagattt gctcgacaag
     4921 gatcagacgc atcaattgat gtggatacgt catttttcca aaagagatct gagcattgct
     4981 tcttttcatg acggcttcgc tgagtcctaa agagccgccg ataacaaaaa caaggtggct
     5041 ttttccttgg atacctaatt ggtcgatttg tttagcaaaa gattcacttg taggattttt
     5101 cccttcaatt gctaaggcat agacgtattc ttgttctttg attttcgcta agatccgttc
     5161 cccttctttt tcttttactt gtctcatttg cgcttcgctt aaattctccg gggctttttc
     5221 gtcaggaact tcgatcaatt cgatttttgc ataggcattc agccgtttga catactcatt
     5281 gataccttgg atcaggtatt tttcttttaa ttttcctact gagataattt tgatattcat
     5341 gttttctctc ctttggtctt catgctcact ttgtttatta taatcaattt tttcgtctat
     5401 atgtttattt tggctatttt tgttaaaaag atttccccaa cagttatgca caacatccac
     5461 aggtttatcc acagaaaaca gtgttatttt accttatttc atcacattca ttaggcaaac
     5521 atacgtttta taagaattta tccactttat acacaggctg tggatatgtt ttatccctta
     5581 tttttccaat cctatccgcg tagttttaca cagtatctgt ggaaaaagaa aatcaaatat
     5641 tttcatctga aaacaatgat ttttcctaaa gtttttcata aaaagttcac agtctcaaag
     5701 tagactaaag tcaacaaata agactatact aacagtagat aaatgaatgt aattggagga
     5761 ggaacacatg gatagaaaaa atgtgacacc aaagatgaaa aaaagtaaaa ataatagcct
     5821 ctggcgtaag ttaggtctgg ggcttgttgg agggatcgtt ggaggactac tgacagctgg
     5881 cattttttat gcagcgatgg gcactggcaa caatagttct actgctacaa gcggcaatca
     5941 gaattctgcc ggtgaaacag ttgtggaaaa tgtcaaagtc aatgtagatt ctgatatcac
     6001 caaagcggta gataaagttc aagacgctgt cgtttctgtt atcaacttac aaagtcaaaa
     6061 ccaatcaagc ggttttggcg gactattcgg gcagcaagaa gaaagtagca gctcagatga
     6121 tagtaatttg gaagcttata gtgaaggaag cggcgtgatc tacaaaaaag acgggaatac
     6181 tgcttatgta gtcacgaata atcacgtagt agatggccag caaggtcttg aagtcttgat
     6241 gaaagacggt acgaaggtaa aagctgaatt agttggaaca gatgcttatt cagatttagc
     6301 ggtgttgaag atcaattcag acaaagtcga aacagttgcc tcttttggtg actcaagtgc
     6361 tctgaaggtt ggtgagcctg cgattgcgat tggttcccca ctaggatctg aatatgcaaa
     6421 ttctgtcact tcaggaatca tttcttcctt gaaccgacaa gtgacaagta cgaatgaatc
     6481 gaacgaaaca gtcaatatca atgcgatcca aacagatgca gcaatcaacc ctggaaattc
     6541 tggtggtcct ttagttaata tcgaaggaca agttatcggt atcaactcaa gtaagatcgc
     6601 aagtacttct gaatctactt ctaatgtaag cgtagaagga atgggatttg ctattccaag
     6661 taacgatgta gtgaatatca tcaatcaact ggaaaaagac ggaaaagtga cacgtccagc
     6721 tctaggtatc acaatggtcg acttatctgc cgtttctact cagcaacaag aacaaatctt
     6781 gaagattcct tcatctgtaa ctaacggtgt catcgtgaca aatgtccaaa cagctacacc
     6841 ggctgaaaaa gctggattga aacaatacga cgtcattact aagatcgatg acaaagaagt
     6901 ttcttctggt gtagaacttc aatctgcttt gtataagaaa aaagtgggag attctgtcaa
     6961 aatcacttac tatgaaggct cgaaagaaaa aacaactact gttgaattga cgatcgatca
     7021 atcggcatta aaacaaaatc aaaatagtga aaattaataa aaaagaggtt gtgacaaaga
     7081 cttgtcacaa cctctttatt ttggaaatgc ttctccgact acggcatcct caggcaatac
     7141 taagattcct ttttttgctg gagcatttgg caaatgcaat tcacgagcgg aacagatcat
     7201 tccatagcta tctacccctc gcaaagttcc tggccagatc atcattccat ctggcatcat
     7261 agctcctggt tttgcaacga caactttttg tccagcttcg atattgggtg caccgcaaac
     7321 gatttgcaat actgtaccat tgtctacttc tgtctcagta atcgataaat ggtcagaatc
     7381 aggatgtggg acgcatgttt ttacgtaccc gacaacgatc ttcggttcat gatcagcttc
     7441 aagctgttct tcgaatcctg cttctttgat caatcgattc aatttcgcta attcctcatc
     7501 tgttaagctt acttggccgt tcccttcgat tgttagatga tcggagatat ggaaaaagtt
     7561 ccaagcaaca acttgacctt gttcatcttt gatccgagca acattccatt tgcgttcaac
     7621 agtgttttct gttcctttat cgtcggctat aataaccatc aaggtgtctc cgacgtgtgc
     7681 tttgttataa gcaaaaatca tgttctttcc tccattatcc tttgctgaga tttatcttaa
     7741 caaaaaattg cattcactgc atgaaatttt tttcatgctt tatttccgaa gaagcctctt
     7801 ctggaacacc atttattcgt aacaagggac tgtgacaaga cttttgtcac agtcccttta
     7861 ctgttttagt taattgatta aaaaggtcgt acaagttcca cttgcttaat tacttggctg
     7921 ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa tgacgtatca cgtagctggc
     7981 tacacttcct gtcataaagc gttcgactgc atttagacct gactgcccaa ccatgattag
     8041 atcgatctca tatttttttg gcaatttgac tgccatggat tcacgaacag aaccatatgc
     8101 aataattcct tctatatttg tgtaatcaac agatttccca tattctttac attcttcgat
     8161 catttccttg gctgtttcag tttgctgatc gactagattt tgatcaatag gtgcatagcc
     8221 cataaagtta taaaattgct gatcgattac gtttgctaca tatactttcc cattattacg
     8281 gcgagccact tctaccgctt ttttgaaagc agcaagtgct tgttcactac cgtcgatacc
     8341 tactaaaata ttcttatatt cttgattcat cttgacccct cctaacagat gtcagccttt
     8401 tgttacactt tctaggaacg attcgatttc ctgttttgtt ttacgatctt tattcactag
     8461 acgacctaat tcttttccgt cttggatcac gacaaaactt gggataccaa aaatgttcca
     8521 ttcagcggct acatctatgt acttgtcacg gtctacttca ataaattgcc aatcagaaaa
     8581 gtcattttca atttcaggca tttgcggttt gataaaacgg cagtctccgc accagccggc
     8641 agtaaagaaa aatacactct ttccagtttc tacgtagcct gctaattctt ctaaactatt
     8701 tgggataatc attctttgtc ctctactttc tgtttatgta tttttcaatt aaattataca
     8761 cctttctttc gaactaacaa acattgatcc tctttttata aaaaaaatct ggcaaaaagc
     8821 aatcttttgc cagattttat agtttcacta tcgcattatt ttccgtatac gatcgtattt
     8881 actgttgatt tatccaaacc tttcagtgtt tgaatcaaca tttctcgtgc agcttcaaaa
     8941 tcgcgaatac tgaacatcgt ttgatgcgta tggatataac gtcccactac gccaatcact
     9001 gtacttggga ttccttcgtt ttgtgtatga gcagcacctg catctgtacc gccttttgaa
     9061 acaaagtatt gataagggat attgtttgtt tcagcgatat ccagcaagta ctcgcgcaaa
     9121 cgaggaagca tgatcaaacc tggatcttgg atacgcatca atgtaccttc tcctaaatgt
     9181 ccgaatgtgc catttttcgt cactgtatca tctgctgcag agcagtctac tgcaaagaac
     9241 agatctggct tgaatttatt gacagaagct ttagaaccac gcaagcctac ttcttcctgg
     9301 acattcgcgc ctgcaatcaa tgtatgacct aatgtttcat tttgcaacgc ttctaaagct
     9361 tctaatacca ttgtacagcc atagcggttg tcccatgatt tactgataat attctttccg
     9421 ttggctgttt tgatcgtttc agcgtaagga acgattgtgt ctcctggaag gacaccgtaa
     9481 ctcattgcct cttctcttga ttcaaaccca gcatcgaaca aaatatcggt tacttctact
     9541 gatttttgtc cgcttgttcc tcgcaataaa tgtgggggaa cggaagaaga aatacatgga
     9601 taattcttac cagtagaggt tttaagtgta aaacgctgag cagaaacaac gtaaggattc
     9661 cagccgccta gaggtacgac tttaaaaagc ccgttttctt ggatttgtgt cagcatgaat
     9721 ccgacttcat ccatatgtgc tgcgaccatt acacggggtg catccgcttc ttcatgatgg
     9781 cgcagaccaa aaatgccgcc caaaccatct aactgtaact catctaccag cggttccata
     9841 tgactttcca tataagcgcg aacatcttgt tcaaaaccgc ttgttccttg taattctgtt
     9901 aactctttga ttcgttgaaa tgttttttct tccatatgct attcgtctaa atttcagact
     9961 aaactcctct cagacttata ttgatctgcg gataaaatat gattttttct gcagaccgat
    10021 cgtatttatt atagcttatt ttttctggta gcggttattt atttcaaaga aaatatgaga
    10081 gtgtggtaaa ataagcacaa ggagtaacaa gtgagaagaa ggaggtatgt ccacatgtat
    10141 gaagaaagcg aactaagcta ttttaaaggc gggttagcag ttggaatcgg tctaggatta
    10201 ttgagcgggg tagcttcaac gatttggtat catcgcaaaa aagtattgtc agcagaccaa
    10261 gtacttgaaa atatcaagtc atcttttcta aaagaaggtg caatcgaagg ttcttggatc
    10321 tcttttgaaa aggaacctat taggaaattc gccgttcggt cgagtgctta tcggggaggt
    10381 ctttctcgaa tagaagatgg cgaaactgtt tattatgaat ttttagcaga tgctcataca
    10441 ggaaccgtat tagaaatttc aagaaaaaaa ggtgcaacag attaatacgt caaaaaagtg
    10501 agagaacgaa tacgcaccca ctattcaaaa gagaggtatc atgtccttct tttgtagtgg
    10561 gtggtttttt agatagagct ttccaaacat atattccagt attttctttt taaataaatg
    10621 atatttagcg ggaaaaacta gcatttcctt tgattatgag gtatttttaa tttagctttt
    10681 gttttctata gttaaggaga gtgaaaaatg gaacaacaag aaaaaattgc tgttctgatt
    10741 ccttgttaca atgaggaagc aacaatttca acagtcattg cagactttaa acgagaatta
    10801 ccagaagcag atatttatgt ctatgataat aattcgaccg atcgaacata cgaattggcc
    10861 gttcaaggtg gagcaatcgt aaaaaaagaa cctcgtcaag gaaaaggaaa tgtgatccgc
    10921 caaatgttct ttgatatcga tgccgactat tatttaatgg tagatggcga tgatacttat
    10981 cctgcagaag ctgtacatgg tttgctcgag aaattacgtt ctggtgaagc agatatggtc
    11041 attggtgatc gcctatcaaa tggaacctac tttgatgaga ataagagacc tttccacgat
    11101 tttggaaata accttgttag aaatacaatt actagaatgt ataaaacaaa aattcttgat
    11161 gtcatgaccg gctatcgagg attcaatcgt atctttgtca aaagttttcc tattatgagt
    11221 tctggttttc aaatcgaaac ggaattgacg atccatgctt tagataaaaa attcaagttc
    11281 gttgaaatcc caatcgatta tcgtgatcgt cctgaaggca gcgaatcaaa attgaataca
    11341 ttctctgacg gattcaaagt gatcatgatg attattaaaa tgtttaaaga ttataagcct
    11401 ttgcttttct ttgggatttg gaccatcttc ttctttttat ttggattggt cacggggatt
    11461 cctgtgattc gcgaatatat gctgacaagc tttattacac gcatcccttc tgcgatttta
    11521 agtactggat tgatgacttt agcacttctt tcattagtaa cagggttgat tttggataca
    11581 gtcgtcacga acgctaagaa agaatatgaa ttgaatctat atcatgttta tcatgaatac
    11641 cacagtagaa agtaaaaaaa ggatcaagac tgcaaaaata atttgcaatt cttgatcctc
    11701 tttttaatta attatttcgc caaacgatag atggcatctg catagattgc tgttgctctg
    11761 aacaagtcat caagactcat aaattcgttt gcttgatgca ttgtatcagt atatcctggg
    11821 aacatcgctc cataagcaac gccacgtttc aacaaacgtc cgtaagttcc gccaccgatg
    11881 atttgttcgt agcctttttc tccagtgtgg tcttcataga cttgcaataa tgtagccacc
    11941 aatggatcat ccattggtac gtaatgtggt tccattacgc gagcacctcg agtaactgtt
    12001 gcgccttctt gccccactgt ttgttcgatg tcactttgca atccgtctac tgttacacct
    12061 tttggaaaac ggaaattcat attgatgaag ttcgcttcct ctgtaccatt ttcttcaaaa
    12121 gcaaatagtc cagcattcat tgtcaatttg cccatttttt catcttcata agcgactcct
    12181 agtttttctc cataaaaatc ttcatggaca taagcagctg ctacatggat aaattgttta
    12241 gcagatccta ggaattcata gttatctaag aacaatgcca agaaagaacc tgcattgata
    12301 ccagattgtg ggcttgcacc atgtgcgcct ttaccaacta attcgatttt tacatacgta
    12361 ttgtccgctt caatcgttcc gcttactgga tttgcttcga tgaattggta aaatgcttct
    12421 tccatcgcga tagctgcatc tgcacttggt acttgaagtg ctgcagttgc tgttcctggc
    12481 accatatttt cgcgaagtcc ggaaacaaaa gatttcaaga cataatctcc tgcattgtcg
    12541 cctttgaagc gtaaagctaa agaaacattt cctttttcac cattgatgat tgggaattca
    12601 gcatctggtg agaaaccaaa atctggttct tcttcatgtt cgaaataata agccatatcg
    12661 ccccagccgc tttcttcatc gctgccgact acgaagcgaa ctttttttga tagtttcaag
    12721 tcaagttctt taatgatctt catcgcatag taagctgcca tacttggacc tttgtcatcg
    12781 cttgatccgc gtgcaaagat ctttccatcc ttgattactg gttcataagg atctgtgtcc
    12841 cagccatctc ctgcaggaac gacatccatg tgtccaaata tccctagcgt ttcgtctcct
    12901 tcgccaagat caatatgccc tgcatagtta tcgacatttt ttactttaaa tccatcacgt
    12961 tcgccataag ctaacatatg tttcaatgcg tcgcgaggtc caggtccaaa tggtgcatct
    13021 ggtgttactt ttgagtcatc gcgctcgctg ttgacacgta aaagattttt caagtcttca
    13081 agcaagtctt ctttacgtgc ttctacttct ttttgccaat caattgtcat aaagttaatc
    13141 ctccacttat ttaagtcttc gtgttcatga taccacaaat tacagaaaaa aatagttcaa
    13201 aaaatctgac aattcctttt cttatctgtt tttctctaaa aaaaatatac gctataatga
    13261 gtaaaaggag gtttttagat gctgataaac gaacaactag tgaaaagagt catcacacct
    13321 cgtccaaaaa tttcacataa aggaacattt ggacgtatcg ttttgattgg cggcaatgaa
    13381 caatatggcg gagcaatcat tatgagtgct ttagctgccg tacacagcgg agcgggtctc
    13441 acgactgtag taactgatga aaaaaatcgg agtgctcttc acagtcatct gccagaagca
    13501 atgattgtag tttggacgga tactgaccag tttctttccg taataaagca agcagatgta
    13561 ctcttgattg gtcccggatt gggtacttct tctaagagtt tggaacgttt caaacaagta
    13621 ctagaaaacc aatcaaaaga acaatggctg gtgattgatg gctctgcttt aagtttactt
    13681 gctgctaatc atcttttgct accctatccc gaacaaactg tacttacacc tcatcaaatg
    13741 gagtggcaac ggttgagttc tctaccgata caagaacaac gggataaaga aaacaaaaag
    13801 aagcaacagg aaatcggtgc tgtgctcgtt ttgaaaagtc atgagacaaa aatttatggt
    13861 caccagcact tctacaccaa tcctttaggt tctgctgcta tggcaacggg cggcatgggt
    13921 gacactttag ctgggatgat cacaggattt ttagcccagt ttcctcaaaa tgaccaaacg
    13981 attgcagcag ctatttatct tcatagtttc attggagacc agctggcaaa agaacaatat
    14041 attgttttgc cgacacaaat cagcgaagca cttccgaaat ggatgaatta tttttgtaaa
    14101 aaatcaatgg aaaacgaggc atgagtcatg gagaaattta caggttatgt gcgagaacct
    14161 ttttattatg aaacagatca aatggggatt attcatcaca gcaattatat ccgctggttt
    14221 gaagaggctc gggtagcctt attggtacat ctagggtttc cctataaaaa aatcgaagaa
    14281 tctgggatca tcatccccgt tctggaagta aattgcagct ataaagaaat gatccattac
    14341 ggagaaaaag tgagcatcca tccaactatc caaaagtata cggggacacg gttggatttt
    14401 agctacgaaa ttgtgggaac tgaagacaaa aaattaaaaa ctactggcag tagtaaacac
    14461 tgttttttac tttctgattc gcaacgatta gtgaagctga gcaaagtcaa tccggagtta
    14521 gatcagttat tccaagcgta cgcttctttg gatgatgtaa attcttaata aagaccgtct
    14581 aagcattttt cttgtattgt gtcgagtagt tcgtataatg aaaagaaaaa agcaggtgat
    14641 ccccatgtat gtcgttaaag taatgcacgg ttacatcgat aaaactggct gtcgcactcg
    14701 ggaaaaaaac ttagacaatc tgctcatttt caaagacaaa aaagaatccg aagcttttgc
    14761 taaaagaatc ggcggtcgtg tcaaaccgat ccaagaggta cgaccagatt gattcaatat
    14821 ttttcttttc gaggctctaa taggcatgcc tatcagggct ttttttgcag actgtgtttt
    14881 tatgcccaca cgatttgtaa gcgatttatg ttataataga ttatatctgc atagaaagga
    14941 aggttaaatg gttatcaata atttgcaaca gcaagcttat gaatctatac gtaaacaaat
    15001 catttattcc gagttagcac ctggaagtaa aatctccgat aaagtgttag aagaacaact
    15061 agctatcggg cgtacaccga ttcaagaagc actgatccaa ctgcgcaatc aagagcttgt
    15121 aaaaaccatt ccacagtccg gcacttatgt ttcaaaaatc gatatccgat ctgcttcatt
    15181 agcccgttat acgcgggaaa agttggaaaa accgatcctg caagaatgca gtgcgaaaat
    15241 gaccaagcaa gatcaagcta aactagaaaa aatactagaa caaaccgatc aggcagtaat
    15301 cgctcaaaat aaaaaagctt tttttgattt ggatactgat tttcaccgaa cttgttatga
    15361 aattgctgga aaacgagaaa tttgggattg gctagaaagt tatagtacgc acttgaaccg
    15421 tttcagatgg ttacgtttga cgatttcaga attagactgg ggtcgagtat tagatgaaca
    15481 tcagacaatg cttcaatcaa tgattgatca taactttgat gaagtcggat ttctttgcac
    15541 gatgcattta catatgatta tcgaggagca agaatacgtg attcataatt accccgatta
    15601 ttttgaagat attcaggaca gacccatcaa ataagaggtt ttggaatcag cgttttgacc
    15661 tgagtattag agaaagaaac ggcaaaatag ttcctcatat tttgccgttt tttttgagct
    15721 aataccgagg gtcagctgat tgcacaccgt ttattgagag gttgtgaaaa agctgccctg
    15781 catcaagtaa taagaacagc caagtaaaaa tagcttctta tgtttttaac ttggctggac
    15841 ttattaccgc agatgcaagc ttttgaacac cgttttccaa gaggtcgtga gcctgccgtt
    15901 catctccgag tcacaaggaa caatttcaaa aaacagcttc tcatgtttgt tgaatatttt
    15961 gacttgtgac cgaggagttg acaggagaac atcgtttatt cggaaaaaaa ggactgtgac
    16021 acgacttttg tcacagtctc tttatttgta atctaaatca tactccgtac atcttttttc
    16081 tatttttttt atttcttcta agatcccttg gctacgaatt tcattatatg gccaaagtaa
    16141 gactgcctct aatacataag gcgaaaaatc gctttgattc atctcttttg aaaaactcat
    16201 cgctgctatc tcgctcaatg cacgaatgtt tgattgatgt gtataaaaag gcaaccgcca
    16261 cagcacaatt ttttcgcttt gcgtataaat tcccttaaca gaagcttcga tgtggtgaaa
    16321 tagttcatgt ccaataatca aagcttgcgc tgtttcttta gagatcgaag ctagtcctga
    16381 acattttaat ttttctaacg gctccgtcat caatcgaatc ccttcatctt ctgcataagt
    16441 tgcaaaagtg attcgttcgt ttatttgctc tgattcacag gaaacgattg gaacagccat
    16501 ttgttcacaa atttctccta catcggcaaa agggtacttt tttttgatgc tacatgccgt
    16561 ttcgatacca caatcgattg attgttgtat aagaattttc tgcttggaca aaggaatctt
    16621 tttccaaaat aaatctcgcc gaaactggta gaatccccaa atttcttcgg ggatctgttg
    16681 taattcgttt agtaactctt ctaacttatg tttgtttgat atttccggca acgacgatca
    16741 cttcttcctc cggttccata gtagatactt ccagatccat caaaagctgt agttgttcca
    16801 gatccgtcgc tgtaaaatca gagactcttg ctcctaagca gagataaaat tctggtcggg
    16861 caatcaattc agtttgataa actgccatat cttcccaaag ttgttcgaca gctgtcatat
    16921 aattggctgc agttgttttc atacatgaag catgtccacg ttggaccttg ataaatcctt
    16981 tttgatcgat gatccgaaga cttccagcat tttttgaacg attctgttcg ccgcatacat
    17041 aaaagaaagg tgtattttcc aataagcaaa cttcattttt atttaatcgc atatcctctg
    17101 cagcaagctc tagtgcttct tctgttgtga tctccttggt tagatcggtt gctttcactt
    17161 ccgtagaacc agtagcgata gccgttactt ttgaagtctg tggatcgatt tctacgtgga
    17221 cttcgatcga ttctggtgta gcgcctgatt caatcgcttt attcatcgct tcatttttca
    17281 acgatcgtat atcttcttta cttggcgaag ggatgatacg ttctacaaca tctcggacca
    17341 ttgcaagtgc tacaccaata gaagaaatca cttcggcgtt ttcaggaatc gaatatttca
    17401 cacccatttt attactaaag taagtaatca atgaggcagc gccgccccct acacctacaa
    17461 gagaaatctg atctttctcc aagtgatact tgtctgccaa ttctaaaatc accggttcaa
    17521 tcttagcata ggatttttcc atgatctgtt ctgcgatgtc ttcaaccgtc gtatgacaat
    17581 aatcagctaa tgcttggatg gcttttcgtg cagatgggac atttccataa gaaaaatgtt
    17641 cttcttgcac taatccaagg acattggctg cacatgtatt tgtaatagtc acttcttctc
    17701 cgtcttccat cactaccttg acgtaatcag cagggtctcc ttctttagga gagaaaaatt
    17761 caaccttagg acctttgatt ttttctgtct ccgtaaatac tgcataatct agaccggcaa
    17821 tatgtgcaga acgtggaccg acatcgatga ttcctgactg attagcccgt accatcgaac
    17881 cgcctgccac acctagaacc cgaacatcta atgaagagat atatgttggg tggccaccta
    17941 caattgaata atcgattgct ggacgtccat ttttaatgac cccgatattg gttgttgttc
    18001 cgcctacttc aaaataaaca ccattagacg ctctcaagta catcaaagag cccatgactg
    18061 atgctgctgg accggaaagc atggttaaaa caggtcgttt tttcatttca ttgatttcca
    18121 ttactccgcc gtctccgcgc ataatcatca aagaaacatt gacccctgct tctcgtacgg
    18181 aatcttctgt agaggttgcc gtatctagca tttttggtaa aatagatgca ttgattgctg
    18241 ctgtccgagt tcttcttgtc aatccatata attttgtgat gtcagaagcc atcgttgtag
    18301 ggatcgattt taccgatgct gcttcataaa caactcgttc tggttcccca ttatctactc
    18361 caaatgccat agaagaaacc aagacctccg ctctttctct ttctaaagaa gaaatcgttt
    18421 ctgaaacacg gtctacattt aagtgtttga caggtaagaa tgcattgaca atctctattt
    18481 ttttcttatt ccctaaatcg atatcgttca atctagtttg tcgttttgct aaaaaacctt
    18541 ctaatccacc ttttgccatc ccgattacgc cgacttttgc tacgtctcct tcaatcagtg
    18601 cattagtcgc ttgtgtggta gaatgtgcaa caaacacaac atcttctgga ctgatattat
    18661 tttccagcaa acaattttga aaagactgaa cgacacctgc ggctactcca cggacatcat
    18721 cgtgggttgt tttcactgaa gattttccga tgatttcgtg cgtcgcatta tcgattgcga
    18781 ctgctttcgt atgtgtaccg ccaacatcaa ttcccattcg cacaaaacgt ttcattttct
    18841 tttcctccat tcatctaatc gtttttatcg acgttttgtt tactaggcgt tcccgtacaa
    18901 taagaacgtg atcaggcata agatcgctcc gatgatccaa ccagttggga tggatttctt
    18961 catatattct cgagaagtta cttttgtata acctaatccc caagcaaccc aagattgtgt
    19021 aatatctaaa tgctgaggag caatcgttgt gattgcaaat aacgggaaca aaaaggctgg
    19081 cgacatcgtt ggattaacag ctaatacgac agctaaaata gctgagcctg aaccgactag
    19141 attcattgga ccgcggaaaa atcctagtgg tgtcaatata gcaaatacga ttgccaagac
    19201 taatggtgat tgcggtatcc aatctccaaa aatagctgaa aaataaggag aagcatacgc
    19261 agctgaatta ttgaacatcg ctaaggtcat caagaaacca accattggcg caacatctac
    19321 tgcgccatct gtaaataatt tcgcaaactg acgacagatt tctttataac ctccgcgcaa
    19381 ttttccagta aacagcaatg cccagatacc tgctagaata aaacctaaga tgattggaag
    19441 atctaaaaga acgaccccta aaactggtaa taatacggct aaccatgaga tcattggtgc
    19501 gtcgccacct tcagctggta cgtttgctgc cattgcatag cgtttctttt tgttcattga
    19561 tatattagcg accgttagta ccacaactaa gcttacaatc atcccaatca taccaatttg
    19621 gaaatagtca ttataagtat agctttcagc cgctggattg aaaccagcat aaatcgtttg
    19681 atattgtttg aaattgacga tattagcaaa gattccagcc ataatcgagc ccataaatga
    19741 gaacagtgca accggtactg aaatacctaa tgacatcaag attggcaaca caataactgc
    19801 aatagaaatc actggtccga ttcctgtcat cgacatgaat ataacagcgg ttacaataca
    19861 tagtaagctc atcgtgattc ttggcttgtc ccctccaagt tcaactacct ttcgaatcag
    19921 ggtagctgcg attccgctat ctactagaac acgcccgaaa aaggcaccaa agaagacatt
    19981 taccaagatc gatttggcgt actcagctgg ccccgtcgta tagatatgcg tcaacacatc
    20041 aattactcct tgcccttcca tcgccgggtt tggtgaaaac gtgtttccga ctagcgctaa
    20101 tcccatccat acagtagcca tgatggcaaa gccgaccatc aagttgtagc cttttacaca
    20161 gtaccagacc atgaaaaaga atgagataac taacaaaata ccaataataa tatcactcat
    20221 gttattcctc cgtccatttt gtttttctga taagcaaact aatatatcag tcaaactcat
    20281 tctaacttat gtagttagcg ctttcattaa agggtgacca taaatgttca gttttcaatt
    20341 aaaaaacctt aattatgcta ttttagactt atacgttatt gtcctgtttc atttgatgga
    20401 ggattgtaaa taaaaaaaga ggaaagaaca taacttttcg ttatcttctt tcctctttat
    20461 aattgtttag cttatttagc caaagcttct tttgcttggt cagccaatgc agtaaatgct
    20521 gctgcatcgt taacagccaa gtcagctaac attttacggt tgatgtcgat ttcagcaact
    20581 ttcaaaccgt gcattaattt tgagtagctc aagccattca tacgagctgc tgcgttgata
    20641 cgagcaatcc acaatttacg gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag
    20701 ttgtatgaat tcattacttg ttcttttgct gttttaaata aagtgtgttt tgaaccgtag
    20761 taaccttttg ctaattttag cactctttta cgacgtttac gagttactgt tccaccttta
    20821 acacgtgcca tgtataattc ctcctaataa aaataaatct tcagattctg ttatttgtac
    20881 ttcttatttc atttttgcta gttgttggcg aatacgtttg aagtcgccac tagaaaccat
    20941 gcttgcttta cgcaattgac ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt
    21001 aaacgcgcgg aagcgtttca atccgccttt accagtacgt ttgaaacgtt ttgctgatcc
    21061 gcggtgtgtt ttttgttttg gcatgactat atttcctcct caaaatcttt cgttatccga
    21121 ctatcggctg tccaacagcc agttagtcat tcaaattact tactgtcgtt tttcggtgcc
    21181 agcgtcaaga acatgctgcg tccgtccatt ttcgcttttt gttccactgt tgcaatatcc
    21241 gcagtttctt cagctaagcg attcaagact ttttgaccaa tttctttatg ggtaatggca
    21301 cggcctttga aacggataga agctttcact ttatcgccct tttccaagaa cttacgtgca
    21361 ttacgaagtt ttgtattgaa gtcattcaag tcgatcgtag gacttaagcg aacttctttt
    21421 acattgatca ctttttgttt tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg
    21481 tatttaccgt agtccatgat tcgcgcaact ggtggtttcg caccaggggc aactaacaca
    21541 agatccaagt ttgcttgttc tgcaatattc aatgcttctg cttttgtctt aactcctaat
    21601 tgctcaccat ctgaaccgat caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc
    21661 atatcctttg ctatggtcat tcacctccaa atattatgag agaaagaaaa gcttaccgaa
    21721 taaacggcaa taaaaaaacg agttctctta cagaacccgc gcgtacttca cctaatagaa
    21781 ccagtgaaaa ctatctagcc cagtgacatt atcaactaag gcgagaagcg ggtgcttctg
    21841 cttttatttc tcaaccatca tagtatagca aaaactaatc tttttgtcaa tgtttttcat
    21901 acagtttgtc ttccttttct gaaaatgatt agattagcag actttcttgc agtgattccc
    21961 gcttctttgc tatactaaag aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt
    22021 ccaccttctc ttcctcttct tacaaaggga gagttttatt tagaagatga ggagatcttc
    22081 tctggtcttc tgatcaacgg gggtgaccaa agctatctcg atgccaaaaa tatggtatta
    22141 agagagagtc atatccaaaa acttacgatg caaaaaacag ttttacgtca ttttgaatgc
    22201 agtaatgtca tttttgaaaa atgtgatttt tccaatctgg agtggattgg tggcagtttc
    22261 caccaagtcg tgtttcatca atgtaaatta acaggtacga attttgccga aagttatctt
    22321 cgagactgta ctttcactga ttgtattgct tcgatggctt cctttagtag tacaaacttg
    22381 aaagctgtct ccttcgatca ttgtcaatta gaagacagcg aattttatga ggtcacttgg
    22441 aaaaaccttt ttttgtctga caatcagttg accggttcga actggtttcg aacttctctg
    22501 aagggattag actttacgac taatttcttc acagcaatcg ctttatctca agattctttg
    22561 gcgggactaa tcgtcaacca agagcaagca cttgtgatcg cccaagccct tggtttgcag
    22621 atcaaagact agatcaacga atccgccgcc acagtgcaat aagcatccaa atagataaga
    22681 ccaaactgat gatcaaggcg attccccatc cgaaaacgga atgggtaaag ggtaactgga
    22741 gattcattcc gaagaatcct gtcacaatag ttggaacggt caagatcagc gaccagaccg
    22801 tcaaaaattt catcgtgtca ttcaagttgt tattcagcaa attattataa gtgccagaga
    22861 gctggtctaa aatctgcgaa gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca
    22921 gcgcatcgtc caactcttct ttttcctttt ctgacaactt gcgataaata gccaatgcct
    22981 tgatttgttc taaaagtact acattttgtt ttgttcctgt aactaagtaa accaaaccga
    23041 tctctaaatc tgaaagttgc agcagattct tgttcgttgt tttttctctt aatttcagat
    23101 taagccgctg acgttcacta ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca
    23161 aaaacaatgt acgaaacaat aaactgtata cactcatgtc tggcattcgt tccaaaagcg
    23221 atcgaattag tcgaatcacg tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt
    23281 cttttaaaat aaaagcgata ggactagttt cataatgatt atcgactttt tgctggtgcg
    23341 gaacatttga tacaagcagt aacgcttcat caaaagaatc gtactctacg cgcgcccgtt
    23401 catttttatc tagagaataa gtcagcatct cgtcactaat gccatattct tcttttaagt
    23461 ttttaacagc tagcacatcg ttttgctgta cattgatcca aaaattacgg tcttgattaa
    23521 aatagtgttt ttcccgcata ctctatcctc ctgacatttg tttccagtat aaagaaaaaa
    23581 gtcgctcaaa taaaggttta ccctttattt gagcaactgt aaatcaaatg gctactttgg
    23641 tttggcgaat ctctgctaat ttttcttcga tcattttcaa gacttcttct tttgcttgag
    23701 gatcttccac aaaattatag cggtctccat cgatttggat ttttggactt tctttgtatt
    23761 cttcatacca ttgatcgtaa cgtctgttca attctttgta ataatcgtat agacttggat
    23821 caaaggacaa ttgttcatac gaacgaccac gtttttcgat acgctccaac atcgtatcaa
    23881 aagatacacg aatatgaact aataaatccg gatgtttttt atgggcagca tagggaagtt
    23941 cttccatcat gttttccagc agttcatcat acacacgaac ttctgtttct gttgctcttc
    24001 ctagatctgc atttagatgg aaaagcagtg aatcttcata aatcgaacgg tccaatacat
    24061 tattgtcatc tttcattgct tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt
    24121 gcagcaaaaa agcatactgt tcagggtttg cataaaataa gggcaagact tcattatcat
    24181 caatcgattc ataaaatgcc tgactatcaa agtgttcagc catcatttct gttaaacttg
    24241 attttccggc accaattgta cctgctaaaa cgatcacgct catttacctc cagctcactt
    24301 catcactaaa tattgtgttt aactccaacc tttttaaata tacccccaat atctagtagt
    24361 gtcaattgta tttttttcta gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa
    24421 gtgttttttc tttagaaaac gtattcttag ttttacttct gagatggttt tagtaaaata
    24481 aaggaggaat gttttttgag acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc
    24541 gttttgggcg ctttattttt tgcttaaaaa tcacttttac gtgagaagga gttagattat
    24601 ggcaaactat ggccaaggag aatcaagcgg aaagatcata ttgatgggcg agcacgcggt
    24661 tgtttatgga gaaccagcga ttgcctttcc tttctatgca acaaaagtca ccgcattcct
    24721 tgaagagctg gatgcaatgg acgatcaact ggtttcttcc tactattcag gaaatttagc
    24781 cgaagctcct catgcattaa aaaatatcaa aaaattattc attcacttaa aaaaacagca
    24841 tgacatccaa aaaaacttgc aactgaccat tgaaagcacg attcctgctg aacgtggaat
    24901 gggatcaagc gctgcagtcg ccacagcagt cactcgtgct ttttatgatt acttagcatt
    24961 tcctttgtct cgtgaaatac tattagaaaa tgtccagctt tcggaaaaaa tcgcccatgg
    25021 taatcctagt ggaatcgatg cagccgctac tagcagcttg cagccgattt attttacaaa
    25081 agggcatcct ttcgactact tttctttgaa catcgatgct tttttgattg tcgctgatac
    25141 aggaatcaaa ggacaaacaa gagaagccgt caaagatgtc gctcacctct ttgaaaccca
    25201 gcctcatgaa actggacaaa tgatccaaaa attaggatac ttgacgaagc aagcaaaaca
    25261 agcaatcatt gaaaattcac cagaaacgtt ggcacagaca atggatgaat cacaatcact
    25321 tctggaaaag ctgacaatca gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga
    25381 taccggggca ttgggtgcca aattaactgg cggtggacgc ggtggctgta tgattgcatt
    25441 agcacaaaca aaaacaaaag cccaagaaat cagtcaagca cttgaagatg cgggtgctgc
    25501 tgaaacttgg atacaaggat taggagtaca tacctatgtt taaaggcaaa gcacgcgcat
    25561 ataccaacat tgctctaatc aaatattggg gtaagaaaaa tgaagaactc atccttccaa
    25621 tgaacaacag cctttcgtta acattggatg cattttatac agagacagaa gtcatctttt
    25681 ctgatagcta tatggtagat gaattttatc tagatggcac cttgcaagac gaaaaagcaa
    25741 caaaaaaagt cagtcagttt cttgaccttt ttcgtaaaga agccggcctc tcactaaaag
    25801 cttcagtaat cagtcagaat ttcgttccta ccgcagctgg attagcctcc tctgccagcg
    25861 ggctagctgc tttagcaggc gcttgcaata ctgctcttaa gcttggatta gatgatctct
    25921 ctctttcaag atttgctcga cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt
    25981 tcgtcgaatg ggaaaaaggc catgacgact taagttctta cgctaagcca gtcccttccg
    26041 attctttcga agacgattta gcaatggttt tcgttttgat caacgaccag aaaaaagaag
    26101 tgtccagcag aaatgggatg cgtcggacag tcgaaacatc cagtttttat caaggctggt
    26161 tagattccgt tgaaggggat ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc
    26221 aacttctcgg agaaacgatg gaaagaaacg gactaaaaat gcatggaacg actttagctg
    26281 cccagcctcc attcacttac tggtctcctg acagcttaaa agcgatggat gcggtgcgac
    26341 aattgagaaa gcaaggcatt ccatgctact ttacgatgga tgctggccct aatgtcaaag
    26401 ttttagtgga aaacagccat ttatctgagg tacaagaaac atttaccaaa ttatttagca
    26461 aagaacaagt aattactgca catgctggtc caggaattgc gattattgaa taaagggaga
    26521 tttttatgat tgaagtatct gcaccaggca aactctatat tgccggagaa tatgcagttg
    26581 ttgaaacagg ccatccagca gttatcgctg cagtcgatca attcgtgaca gtcactgtag
    26641 aatctgcacg aaaagtcgga agtatccaat ctgcacaata tagtgggatg cctgtacgtt
    26701 ggacaagacg caacggagaa ttagttttag atattcggga aaatcctttt cattatatcc
    26761 ttgctgctat tcgcttgact gaaaagtatg cacaagaaaa aaacattctt ttatcctttt
    26821 atgatctgaa agtaacaagt gagttagaca gttcaaatgg ccggaaatat ggattgggtt
    26881 ctagcggtgc cgtgaccgtt gcaacggtca aagccttgaa tgttttttac gcgttgaatt
    26941 tatctcagtt ggagattttc aagattgcag cactagccaa tttagcagtt caagataatg
    27001 gttcctgcgg cgacatcgct gctagctgtt atggtggctg gatcgctttc tcaaccttcg
    27061 accatccttg gctccaagaa caagaaactc agcattctat cagtgagtta cttgccctgg
    27121 attggccagg tctatccatt gagccattga ttgctcctga agatttacgt ttattgattg
    27181 gttggacggg tagccctgcc tctacttctg atttggtcga tcaagttcac cgttcgagag
    27241 aagataaaat ggtggcttat cagcttttct taaaaaatag tacagaatgt gtcaacgaaa
    27301 tgatcaaagg gtttaaagaa aataatgtaa cgttgatcca acagatgatt cgaaaaaacc
    27361 gacaattact gcatgattta tctgcaatca ctggggtcgt catcgaaacg cctgctttga
    27421 acaaattgtg taatttagct gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg
    27481 ggggcgattg cggaatcgta attgttgacc agaaatctgg cattcttcct ttaatgagtg
    27541 catgggaaaa agcagaaatc actccactgc cgttacatgt ctatagcgat caaagaaagg
    27601 aaaaccgatg aatcgaaaag acgaacatgt ttcactagct aaagcctttc atgataaaca
    27661 aaaaaacgaa tttgacttcg tacggatcat ccataaccct ttgccgcaaa tagcagtttc
    27721 tgatgtcgat ctaagtacac aggcagttgg gtttacacta agcagcccat tttatatcaa
    27781 tgcgatgact ggcggaagcg aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc
    27841 aagagaagca gatttgatga ttgccactgg ttcagtcagc gctgccttga aagtcccttc
    27901 tttagccgat acttatacga ttatgcgaca agaatatcct cacgggaaaa tcattgccaa
    27961 catcggtgca gggacttctg tcgaaagagc caaagaagct atccgattgt ttcacgcaga
    28021 tgccttacag attcatttga atgcacctca agagttggtt atgcctgagg gagaccgtga
    28081 ttttaccaat tggaaagtac ttattcaaga aactcaaaca gccattgacg tccccctcat
    28141 cgtaaaagaa gtcggttttg gcatgacaag agaaacgctc aatgatctag ctgctttagg
    28201 agttcataca gttgatatca gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc
    28261 ccgtcgctcc aagcgggaac tgagttattt agctgactgg gggcaatcga cagtctcttc
    28321 attgcttgaa gcaaatgaag cagacacttc tatggaaatc ctagcctccg gaggtatccg
    28381 caatgcctat gacatattta aagccctttg tctcggcgca aatgccgtgg gtacttcagg
    28441 aaccgtactg acgcatctga tgaatcacgg cgtagaagaa actattatac tgatgaagca
    28501 atggcaagaa gagcttcgtc tgctttatac tatggtcggc gcaacgaata ccgcggccct
    28561 acaccaacaa tctctgatct tttccggacc agtgaaggat tggtgcgaag caagagggat
    28621 cgacttagtg aagtatggaa gacgcacaga aaagagcgtg ggacaaaagt aaagaacact
    28681 tttgtctcac gctctaaaca cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata
    28741 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag
    28801 ttaaatgctt ctgtcccagc ctcttttttc gaataaatga tgtttaatca gctgtccttc
    28861 ggtattcgtt caatccgctg atttcacaac ctctccacta aacagtgtac taaagctctt
    28921 ttaaggttgt tttccgatat aggcaagtat gcctccgtcc acatacaaga catggccgtt
    28981 aacgaaatca gatgcgctag aagccaagaa gacagatggt ccagccaaat cgattggctc
    29041 gccccagcgt gctgctggcg tacgtccaac gataaattga tcgaatggat gacgttcacc
    29101 gttttcttgt gtttcgcgta atggagcagt ttgcggtgta gcaatataac ctggtccaat
    29161 tccattacat tggatattgt attgaccgta ttcagaagcg atgtttttcg tcagcatttt
    29221 taatccgcct tttgccgcag cataagcact gaccgtttcg cgtcctaatt cactcatcat
    29281 ggagcagata ttgatgattt taccgccgcc tttttcgatc atatccggaa taactgcttt
    29341 tgaaacgatg aatggggcat ttaaatccac gtcgattact tggcggaagt ctgccgcatc
    29401 catttcgatc atcggtgtcc gtttgatgat ccctgcattg ttgactagaa tatcaactga
    29461 acctacatct tctttgatct gttgaaccat cgcttgaacc gcttcttcat cggtcacatc
    29521 gcagacataa ccttttgcat cgatgcctgc ttctttataa tgcgctatac cttcatcaac
    29581 tgattcttga ttcaagttat taaatacgat ttttgctcct gctgtagcta aagagcgtgc
    29641 aatctcaaat ccaataccat agacagcacc ggtgactaat gctacttttc catctaaacg
    29701 aaacatatcc atactaaatt ccatcatttt ctctccctta tgcttatttt aattgatcca
    29761 tttgcaccat atccatgtct gtataggtga tattttctcc gcacattgcc cagatgaatg
    29821 agtaattgct tgtacctaca cctgaatgga tcgaccagct tggcgaaata atggcttgtt
    29881 cattgtctac aactaaatgt tttgtttcat ctggtttgcc catcatgtgg aacacacgtg
    29941 tatcttcatt ggcataatca aagtagacat aagcttccat acgacgttca tgtgtatgac
    30001 aaggcatcgt attccaagcg cttcctggtt ctaaaatcgt ataacccatt tgtaattgac
    30061 agctttcgca tacatttgga tggatgtatt ggtagatttt acgttcattc agtgtcaggc
    30121 cttcaccagt ctccattggt tttacttggt cgatactgat ttttacattt ggatatttat
    30181 gatgagctgg aacagaacta atatagaatt tagctgggtt ttcagcatct tgagaagaaa
    30241 atacaacatg tcgtgtttct tttccgatat agtatccgtc ttgttttttc atttcttctt
    30301 tttgcccatc gatctcgatg aatcctggtc caccgatatt gatcacacct agttcacgac
    30361 gctctaagaa gtaatcaaca cctagttctt tgtctaatgt gatttctaat tctttttcag
    30421 taggtgtcac tccaccaaag atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc
    30481 ctggttcaaa taccttttct actaaaaatt cttttctcaa ttgttccgtt gaataatgac
    30541 ggatatcttc tggactgtgt gtataacgtg tttccatttc ttgcattttc ttctactcct
    30601 tctatcgaat gatttttcca gagccgcatg ctaaaaattg tgcgacttct tcttgactaa
    30661 attggttgca gtccccatgg atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa
    30721 tgatttcttg cggagacata ttggataaaa tcccatggag cacgccacct gcaaaagcat
    30781 ctccgccacc tactcgatcc acgattgggg tgattctatg gacttgggat tccacatatt
    30841 catggttcat ccacaatgtc cccttcaatt cattttcact tgctgaatga acggtacgct
    30901 tggtcgaata aaacacctga atattcggga acttttcctg cattttttga taataataaa
    30961 ttagaggttg ttcttctgct tctggcgctt tctcgatacc taacaaataa agagcatcca
    31021 tttttccagc tgagcagata tctactaaag gtaaaagctg atggattact tcgctggctt
    31081 cttgctgtgt ccataatttt ccacgatagt tgatatcgaa gctgatcagg cagccagctt
    31141 tttttgcagc ttctatcacc tgtttcgtca ttttttgcca ttttttagac aaagctgggg
    31201 tgattcctga aacatgaaat atatctattc cttggaataa ctcctctagc gaccattcca
    31261 aatgatccat ttcagcaaaa cttgatccgg cacgatcata gatcactgag gcggcgcgtt
    31321 cgccactacc agtttccata taataagtac ctagtcttga tccaccagat aatacctgtt
    31381 tcgtatctac tccataacat tgcaagtgct ttttgactgc ttttcccaat gcgttatcag
    31441 gtacttttgt agcaaaaatc gcttcatgtc cgtaattagc taaagaaatt gctacgtttg
    31501 cttcccctcc gccataatgg gcagcgaatc gttcacttga atcgatacgg gttccctgct
    31561 ctgtggacaa tcgcaacatg atttctccga gtgtcactac ttttcccata agcagctacc
    31621 tcttgatttc tttcattttt gctgcatact gttgcgcgac ttccgttact ttgtcaaaat
    31681 ctcctgtagc tgcgggtgct aaaaggttcc cacctactcc gactgttacg acaccagcat
    31741 cgaaccactc tttcatattg tccagactca caccgccagt tggcatgata ttgacttgag
    31801 gcatcggcgc cttgaaggca gaaatgatac ttggtccata agcgcttcca ggaaatagtt
    31861 tgacgatatc taccccgctt ttcagcgctg ttttgatctc ggtaatagtc atacatcctg
    31921 gtaagtaagg aatttgatac agattgcaca tctctgccgt ttcttgatca aaggaagggc
    31981 taacaatata ctctgcgcca gctaagatag ccaagcgagc tgtgacagcg tctaatactg
    32041 tgcctgcgcc aatcactatt tctggttggt ctttgtagaa tgacaaaagt tctttgatta
    32101 cttgatctgc ttgagggact gtaaaagtca actcaatacc agttagacca cctttgatga
    32161 ttgcgtggca agcattcaaa gcttcttctt tgctttttcc gcgtaccacc gcaatcactc
    32221 cggcgttttt caatcgagat aaaatatcaa tttttttcat cttctccacc tatttcctat
    32281 ttagaaattt tattttataa cttggaaatc tatacccata ttatacggca gaaaagaaaa
    32341 attgcaaacg cttttttcat tatttttgaa ttctttgctc gatttcgctt tttgtttgct
    32401 tgagggcact gattatttgt tcttttgtct catctgttaa acgatatttc ggcatactta
    32461 cactaaatgc accaatgatt tgttcgtttt tcacgattga ggctcctata caaaaaatat
    32521 cacgttcgat ttcttcatcg tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct
    32581 ttttcaaccg taatgcattg gtgatcgtat attctgtgta agggatcaac ggatgctttt
    32641 caagataatc agtgtactgc tcttcactga attctgctaa tacggctttg cccatcgcag
    32701 aattgtataa tggtcgggtg attccgattt ttgaagacat gcggatcgtt tgatgttttg
    32761 gatctagttt gttcacgtac aagatctcat tgttgtccaa tacccctaag tggatagtct
    32821 catcgatctt ttgctgcagc tgttctaaaa acggtaaagt caactctacc aaatcgattt
    32881 gttcaatgtt tttattcgcg tagcgaataa gctttgcacc taaacgatag tttttctctg
    32941 aatttttatt tacgtagccg atcatcaaca aagtatctag tattttcaag actgtagaag
    33001 aggtcattcc cacacccttt gcgatcgtct gcaaagagac atcttggttt tccgctaaat
    33061 agtctagtat tttagaggct ttgatcaata cagttccata aggcttttta ttttccatat
    33121 aatattctcc tatttcttta taagaaaaat tatagcggaa ataccatcaa tttaaaagag
    33181 atatccagat ataaactacc atttctcttt acaggcaatt taatgttgaa gatccgcttc
    33241 gtttttacat caaactttga tataaagcaa tgatttcttc tactgtcgtt tctcttggat
    33301 ttcctggtgt gcatacatct cgcaaagcat cttctgcaat tgctggaata tctgcttctt
    33361 ttacacctaa ttcagaaatg gtttctggga ttccaaccgc tttgctcaac cgatcgatct
    33421 caccacaagc cgcttctctt acatcttcta aagacatttc agctgctcct ttaatcccta
    33481 gaacaagagc gatctcacgg tacttttctc cagtatattc tttgttatat ttcatgacag
    33541 ttggcaaaag tgctgcacac gctacgccat gaggaatgtt gtaccatgca cttaacgggt
    33601 gagccatccc gtgtaccagt cctaaaccga catttgagaa gcccattccc gcaatatact
    33661 gtcctaatgc cattttttct cgcccatttt gatcgccatc tacagaagct tccaaagaat
    33721 ggccgatgat ttcgattgct tttaaatgca gcatatcagt catttcccaa gcgccttttg
    33781 tgataaaccc ttcaatagca tgcgtcatag catccatacc tgttgatgca gccaattttt
    33841 tcggcattcc catcatcata tcgctgtcga tgaacgcgac gattgggata tcatgaggat
    33901 caacacaaac aaattttcgg tgatttactt catctgtgat gacgtagttg atcgttactt
    33961 ctgctgctgt tccagaagtc gttggtatag ctaatatagg taaacatgga tttttcgtat
    34021 cagcaacacc ttctaaacta atgacgtctg aaaactctgg attcgtcaaa ataatcccaa
    34081 tggcttttgc cgtatcgatt ggcgaacctc caccgattgc gatcaaacag tctgcctctg
    34141 cctctttaac aaaagccact ccatctttta cattttggat cgttggatta ggtacgatcc
    34201 cgtcgtataa agcataagcg attgctgctt tatctagaag ttctgttact tttgttgcta
    34261 taccgtgctc gatcaatcct ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct
    34321 gaaattcagg gacgatcgac tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa
    34381 tcattcgatt actcattttc tattcctcct aatttgtatt atttttcaca ataaaaacga
    34441 aagaacccca agcaaccggt tgcccagttg cttgttgcta cagttcaaag actggcatca
    34501 attcccaact gactggagag gcatctggat ttgtctccat gatatcttcc atgtacttcc
    34561 accatttttg attgatagat gtatttgcca ttttttccca taacttttca tcttcgatca
    34621 ctaagtaacc aaataacaaa cttgtactcg gatcaagata gatcgaatag tcgatgaccc
    34681 cgtgttcacg aagtgctgta cgcatttctg gccaaagttc atcatgtctt tttttgtatt
    34741 cttcttgttt ttctgggtaa actttcatgc aaaaagcttt tttcaccatg tcagacacct
    34801 gctttcgtat gaacttcctc caaataacct gcttttggag tgacaccaaa cgcatctgct
    34861 aaatgccaca ggttttcgtc cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac
    34921 gtatagactt ctgctgcctt ttctgctgtc tcgatcaaac caaagacttc atccatgtct
    34981 tttcctgacc cgtagattcc atgttgtggc cataaaacca atcgagtttc tttcattttt
    35041 tcagctgttg cttccccaat ctcatttgtt cccggaacca tccacggaat gatggaaatt
    35101 ccttctggaa aaacaactaa gcattccgta cacatttgcc atagcgtacg actaaaggaa
    35161 cgttcgtcta attcatgcgt aaaactcatc gctaatagat gactagcatg gcaatgcata
    35221 acgatccgat tatctgggtc tacttctaaa cgggcgatat gactcatgaa atgacttggc
    35281 aactcgcttg taggaaggca ttcgttttcc agcccccaca aaagttccag tgttttgcca
    35341 tcttcagcaa ctcgaacgat tccaagattc tcggcaggtt cgctcgctac atttttaaaa
    35401 tatcttcctg aaccagttac aataaaatag catcctgcta aacggctggc atcaaaaatc
    35461 atcgagatgc ggcggatcac tttttcagga tccaagaacg gtgtgatctc ttcttctttt
    35521 agtaaataag aaatattccc gccatttctt tcatcccatc ccaagcgata aagattcgac
    35581 gtcgtttcga tcatttcttt cacataaggc gcttgtaaaa tatctatttt tttcaatata
    35641 gccacactcc tattttctta attccaacac gtctttttga tattcctgga cttcttccag
    35701 ccaatccaaa ccaactggaa cttgattttt gtaacagaaa taattccata catcggcata
    35761 cgggaaatct ttcaattctt ccgtataagc cattcttgaa gtaaagtcaa aagccagctc
    35821 catttctttc aatcgctcaa ctggttccag catagctttc agcagtgctt tttgtgtatt
    35881 tctcgtaccg atgacccaag cagcgatacg gttgatcgtt gcatcaaaga aatccagacc
    35941 gatatttgtt ttttccaaca attcgttacg gactaattct ttagcgattt cctgcaattc
    36001 atcatccatc aaaaccacat ggtctgaatc ccaacgcact ggacgggaaa catgcaacat
    36061 gattcctttg ctgaataaag ctaatgaaga taatttattg gaaatcactt ctgttggatg
    36121 aaaatgtcca gcatctagac aaacaagttt gtcacgagtc aacccgtaac ccatataaaa
    36181 ttcatgggaa ccgactgtat aagcttcagc gcctaaacca aatagtttgc tttctactgc
    36241 atcttgtaca taccgttcat ctaactcttc tgtaaaaatt tcatcgagtg attccattaa
    36301 acgttcccgt ggggtaaaac gatcaatcgg attatctttc atcccatcag gtacccaaaa
    36361 gttattgata cttgtttgcc cagtttcttt cccaaagtac tccgcgattt tgcggcttct
    36421 tttcccgtgt tcgatccagt attcacgaac ttctttatct ggatgagcca atgtaaagcc
    36481 gtctttcatc attggatgcg aaaagaaagt cggattgaag tcaagaccga tttcttcttt
    36541 ttttgcccac tctacccatt tttcaaaatg tttcggctca atttcattta gatcgacaga
    36601 ttcttctgta tccaaataga tagcatgaag gttcaatttg tgtttgcccg gtatcaacga
    36661 ataagccttc tccagatctt gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt
    36721 ccctgtcgcc atgattccac cggtcaattc gccatccggt gtcaagaaac ctttgacatc
    36781 atctccttgc cagcaatgca tagaaatttt gatgtcctgt atcttttcta gtacagcatc
    36841 tgtatctaca ccgatagttg catatttttc ttttgcttgt tcgtaatttc tttcaatcgt
    36901 gttcatacga ttactccttt gattttattc acgtaccatt ccatcgtgtt ttgcttattt
    36961 tcatctgggt aaaaagtttc acatggaaac gactgtttga ttacatttct tgcttgttct
    37021 atattcgaaa actgacctgt actgagcatt tgcatggcaa cattcccgat tgctgtggct
    37081 tcactaggtc ctgcttctac aatgattccg gcataatctg ctgtcaattg attcagtaga
    37141 cgattattcg atccgccccc tacgatgtgc agcttttcca atttcgttcc agttaagtgt
    37201 tctactttga tcagctcttg tgcgtaacaa agagcaagat tatcatacac acaacgagcg
    37261 atagctcccg atgagacagg gactggctgc tctgtttcaa aacagtattg ctgaatctct
    37321 tctatcatat ttgggggatt caaaaatcga gggtcattga tttccatcac ttgctgcagc
    37381 ggtttttctt ttgcagcaag ctctgccaat tccgcataac tgtactcgta ctcttgcatt
    37441 cgggcaactt cctggatcaa ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta
    37501 tgggcacccc attcattcgt ataattttca gaaaaagcat tgcgagaaac attggcgatc
    37561 tttgtttcaa tccctaaaag cgaccatgtc ccactgctga tataacccca acgttctcct
    37621 tgacctggcg ttcctaatac agctgacgcc gtatcatgac ttgctactgt gataaaggtc
    37681 acatctggca aatcatattt tgaaaattgt tctttttgca gatctcctag tatcgttccc
    37741 ggctctgtca atggtggaaa taattccttc gagacaccga ttttttcaag aagttctatt
    37801 tcccattctt ttgtccctgc attcagcagc tgcatagtag atgcattagt tttctccgtc
    37861 accatgttac cagtaaagac ataccctaaa taatcaggta tcagtaacaa tttatgtgct
    37921 tttgccaaat tttctttttc ttcagcaaac aattggaaca aggtattgaa aggctggatc
    37981 tgaatccctg tctgctcata caacttatct agagaataga tttcagaaaa ctgttctact
    38041 gctccattgg tacgcttgtc ccgataagca atcggatcat ttatccgatc cccttgttca
    38101 tcaatcagac agtaatcgac tgcccatgta tcgatccccg caaaaaggcg atcaatccct
    38161 gtctgcttta atttttcaaa tccaatcaaa atctctttga taagataatc gatcttccag
    38221 cgatcgtgac cgtctttgtt ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc
    38281 aaagaacctt ttgcatccct cgtacttttc attagtcgtc cgcttgatgc accgatatcg
    38341 atggcaacaa atgttggatt catttctcca cctcttttac tttattgttg attagcactt
    38401 tattaataaa gcgttatcat ttattgacaa tgctatcata atacagaatc ttatattttg
    38461 cttcacacta ttttggtata taattgcatt atcttgacaa gttgggagtg tttacatgga
    38521 ttactgggaa aaagccaacc actcatggtc ggaagactca aaaagagtga tcttgacgcc
    38581 tacacaaaga agcaggaatt tattttttta tatccaagaa atcggttact tcaaagcacc
    38641 aatcccttac tatactgaac gagcacattt accttcttac ttgataaagt ttactttgtc
    38701 tggcaaaggt tcgcttacat ataatgataa tcagcaacag ctaaaagctg gagacttatt
    38761 ttttattgac tgccaagaat atcagcgtta tcaaacgatc agcgaagaac catgggaaat
    38821 ggattggatc catttgaatg gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga
    38881 cggccataac accttccatt caacaggaga tccatcagaa aacaagatcc accatttgat
    38941 cacagaattg atccacatgc aacaaggcac gaatgcccgt accgatttcc agtcctctgt
    39001 agctatccac gagttgctga atgaactgat tttgcaaaag tatcagctag atttcgaaga
    39061 agaggatatt cccgcctacg tttacaaact gaagcaatac ttagacgaac atttcaaaga
    39121 aacgattaca cttgatcaat tagaacaaca attcttattg aataaatatc agctgagcaa
    39181 agatttttcc aaatttatcg gttctccacc aatcgattat ttgatcagca aaaaactttc
    39241 ttacgctaaa gaccttcttc ggtatacaga atttacggtt cgaatgattt ccgaagaaat
    39301 agggattgag aattttgctt actttagccg cctgttcaaa gcaaaaacag gactatcgcc
    39361 gagagacttt cgtaaattca gctgattcat ataatatgta cgtcattttt tcaaaatatt
    39421 ttgaaatata tagataaaaa taaaataacc gcagtttctg cttctatgta aacaacagca
    39481 gctactgcgg ttatttttta gtgatcattc tcattgttca tcttcaaaat gttcataatg
    39541 aaccaactgt ttctgtgtat cacgtttcaa aaattcttta ggcgttatgc cgaactctct
    39601 cttgaatgct ttcagaaaat gcggataaga agaaaaacca cttttactat atacagcact
    39661 agagctgcgg tattctcgca acaagtgctt ggcatagatc agctttttct tcattacata
    39721 ctgatgaaac gtaaatccag tgtacttttt aaattctcgt gtgatatggt atttgctgac
    39781 aaaaaactgc ttttccattt cctctaaaga aagcggatga gataaatttt ttgagatata
    39841 ttgtaacatt tgttccacac gctcattagg aactacagca ttctgttctt gcgcactctc
    39901 ttctttttgt acaacttgat tgagaaaaat caagtattct accagagctt gttcatattt
    39961 tatgtcttca ccgtaaattt ttttatcagg aggatggtcg ataaatgata atttttgctt
    40021 caatacttcc tgttctattt gcaatacacg actcctcttc tctcccattg gttgaaaaca
    40081 agccgataga ttagaccatg gcgtggaacg actttctaga aaagaagggg tgacaaagat
    40141 atagacacgc tcgaatacat ctgaagattg gcggacgata cgatgcaaat cattggaatg
    40201 gatgagcagc acacttcctg ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc
    40261 ttctcccttt aatgtagcat ggatctcata gaaatcatga tgatgataga tcgttttatt
    40321 ttggctgacg acatctttga catgatagat ttcatactga tcagaaacca tcgtattgac
    40381 ttgttcaccg ctgattttct tttggagcat gtgccgcacc tccttttgtt cacttatgca
    40441 caattttatt ataccacgtt ttttgtaatc aaaaagagct gcttttatat actcttataa
    40501 aaaagaagag acgtggcatc ttctagcccc gccccctcca ttctaaatat tagttaacca
    40561 acgtcttctt tacattattc aagaagactt cttccataac gaatcccatc atcttttgtt
    40621 tcttcaagaa ccacatacag gtacggacga ttcttcatga tgatttcttt gatttttgtg
    40681 tactcgatca caccgtctcc caaatttacc ggcacgattt tttcctgttc gacacgaaaa
    40741 tctttcaaat gaaccgcaca tatcttttca ccaaaacgtt caaaagcttc ttctaccaat
    40801 tggacttgtt cttgataatc actacttgtg atcaaattgg tcggatctaa aatgattcct
    40861 agatagtccg aatctactgc ttggatcagc tgctctaccc ttgctaaaga atacaccgga
    40921 tgattcaaac ctggttctat ccccaccatc atttggtatt tttctccagc ttttaccaag
    40981 cgacggataa cggacacagc ttcagcaaat gcttcatctg tgaaattttc ttccgtatat
    41041 tggatttccg gcaaaacaca tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa
    41101 gcatagcgaa gatactgctc gaactttgtc agcaactctt gtcgtatcgt taaatctgga
    41161 tgaatcatat tgatatagca acttaaaatt cctacggtca cttcttgctt ttctaaaact
    41221 cgtttgacat aatttcccat cccagaattg atctcatttg cgccagaagg tatttcagga
    41281 aacgaaagac ctaaggcgag ctgcagtgta tgaatccctt gttcttttgt tttttctgca
    41341 agatcttcta tcgtttggac ttcggaaaga tcatgtcctc taattgtaat gttcaacata
    41401 actattctcc taactcagca tattgggtag ccacatgacg atttgcggga agtaagtaat
    41461 caaaaacaag acgactaaga taaccccata aaaaggaaga agcggtttca gcattgcctc
    41521 tgcttttacg cctcctacac ttgcaccgac atatagtcct gtacctactg gcggcgtgat
    41581 ggagcctaca cacagattca tgattgaaaa caacccgtaa tgtacaggat caacccctac
    41641 acttgtgacg attggtaaaa atattggtgt aaaaatcaag atagcaggcg ccacatccat
    41701 aaacataccc accagtaata acacaagatt gactaataac agaataataa atttattatc
    41761 cgttacgccc ataatcaacg aactaattgc ctgaggaata ccggtaaacg ccatggcgaa
    41821 agacatcatg gaagaagtag ccaataagaa catgatcgtc cctgacattg ctactgattg
    41881 aaacaacata ttaggtatat cttttagttt taccgtttta tagtaaaaca tagcaatcaa
    41941 cagggagtaa atgacagcta tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat
    42001 accaccgata atgatgacaa tcaatagaag gctaggaatc gcctctttta ttatttttcc
    42061 agcatgagcc atctgtcctt tttcgacaac agggtattta ttctttttag caacgatgaa
    42121 tgctactagc atgattccta gcgcccacaa agcgcctacg agataacctc ccataaacaa
    42181 agcagagatc gaagcaccac cagcaacaag tgaatacatg ataaaagcag tacttggagg
    42241 aatgaccata cctgtcggag ctgacgcgat attgaccgct gtcgcaaact tccggtcata
    42301 tccttcttcc acttgctgag ggatcaacac tccgccaatc gcggtagaag cggcaatggc
    42361 cgagctagag atggaaccaa ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc
    42421 cggcacgcgc ccgacgaaca acatcgcaaa atccactagt ttcttagcta tccctccatt
    42481 attcatgata atgcccgaga gaacgaaaaa cggaatagcg atcagcgaaa aactattgat
    42541 acttcccacc attttttgag ctgaactaaa agcagaaaca tcaaacggta taactaacaa
    42601 taaagtcatc atcgatgaga atgcaatact tatagcaatc ggcatcccta atacaagcaa
    42661 cacaacaaat acaaaaaata acactaatcc ggcttctatt gccattttat tcctccattt
    42721 ttatattttt tcttgaattg ctggcttgtt gcttaatgta tcgattagat tgatcaggct
    42781 atagataatg atgagtaccc ccgatacagg tactgacaga taaacatatc ccataggaat
    42841 acttaaagaa ggcgagatct gccccattgt caatgtgaca gcattccatc cgccatacac
    42901 catgatcatc gcagcaaata ccagaaataa actttgaaca acaacttcta aaattttact
    42961 ttttttgcca gttaattttt cacttaaaac cgttacggct aaatgtgctt tttgtcccac
    43021 aacatacgcg gaagacaaca tggcaaacca gacaagaccg aatcgaacaa gctcttccgt
    43081 gaccgtattt ggatttccta aaaccgttct ggaaaatact tgccataaaa caacagcgac
    43141 catcccaaca agcaaaacag ctcccaggat ctctaatacc ttgtttaaaa tcattttcat
    43201 cacagctacg ctccttgctc ttggatcttt tgataaaggt ctttgtaggt aggatcgttt
    43261 ttgaatatat catgaagtgg ggcaactgcc tctcgaaacg gtgtattatc tacatcattg
    43321 aattggactc catgttcttc ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa
    43381 gcttcaattt caaaaaccgt tgattctttc gctgcttctt caatggcttt ttcctgctca
    43441 tcagttagct gttctttgat ggcatcattc atgatgatga tatctggcac acgtgtatgc
    43501 tcatcatagg aatagtattt agccacacca ccatgtccag ctgtatacaa gacaaattca
    43561 ttgttttctg atccgttgat caaattggat tgaagagacg tgtacacttc atcacttccc
    43621 ataggtacgg gcgaaccgcc taaaagctct accattttga tagcggtttc gctttgcatc
    43681 acacggatct tctttccctt caaatcttcc ggcgtatgga tcggaccgtc taccatataa
    43741 aagctgcgct gaccagaatc ataataggtc aatccggtaa atcctaaatt tttggtcgat
    43801 tggtagattg gctccattat ttcttcatta tccatcacct taaagaaatg ttcttcatca
    43861 ttgaatagat aaggaactga aaaaatcgaa taatctttag agaaactttc caacgctgag
    43921 gcacttactt ttgtgaaatc tactgctcct gactgtgtca attcgatcag ttcacgttct
    43981 ccacccagtt gtccatctgg gaaatactgg atctgtacgg aaccatctgt tttttctttt
    44041 actaaacgcc caaattcatt cattgcatca attactggct ggctgttgct tgcataagca
    44101 aagcgcaagg taactatctc atttacatct gcatctgcga cagttgccac actagaatta
    44161 tttccgcagc cgcttagacc tagcagacct aggctaagcg aacttgctgc aaacagcagt
    44221 gatgtttttt tcatatgggt ttcctttctt ttaacaataa gagacgagca attctgtcaa
    44281 acaaagcacc gttaacgact gtccataagg cattgctgtg attccgatat tccgataaaa
    44341 atctaaactg tcccccattc ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc
    44401 gatttgttca agcaatcctt ggatagcacg ataagcgact tctttatatt cttgtggtaa
    44461 ataacgttta tggaccgctt tcaaaatacc gtaagcaaat cctgcagttg cagaagattc
    44521 tacgtaagaa gaaggatcat ccaacaacgt atgccataaa ccgctttcag actgataagt
    44581 ttttaatgca cgcacttgtg cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc
    44641 tttcgataat tctaagattt caatgatctc tggaatagca atcgtcaacc agcagtttcc
    44701 acgagcccaa aatgcttcgg catagttatg atttccttca aacgtccagc cgtgatacca
    44761 caatcctgtt cgtttatcca tcaagtattt gatatggatc ataaactggt aacgagcttc
    44821 ttctaaatat tctgggcgat tcaacagttt cccgattttt gccaaaggaa gcactgtcat
    44881 catcaatgta tcgtcccaca actgattctt attttccggt ccatatgtgg catgctgtaa
    44941 cccgtcttca tttgtccgag gcatgtcatt catgacccat tctgcccatt gttctaaata
    45001 aggcaaatag cgtgaatctt tcgtgtcttc atacaagtaa gccatagtca atagcggtgc
    45061 cattgtattg acgtttttgg gcggagcgcc ttcttccata cgatcctcaa accattcatt
    45121 gaccacttga taagcgcgag gattttttgt catctggtaa tttttataaa taccatatag
    45181 accgacacct tgtggccaat tccacactgt ccagcttttg tcatctactt tcagtccatc
    45241 aaagtccaac aaaaattcac cattatcgtc tttgatcgtt gtcaggtttt caacgagcag
    45301 gtcaattttt tctttcacta attctttagg tacaaaaatc tcttctttaa taatcatctt
    45361 tacgccacct ttttaaacgc tttcatttta tatccttaat atagcagtaa taccgtccac
    45421 tataaatatc gctttttgaa caaaaactgt ctaaaattga cctaaatgtt aaataacaaa
    45481 agtaactttc agttacttgg aaattaaaaa aagaacttgg agaaacaacg ttttccaagt
    45541 tctttttcat ctattttctt atcttttttc tacgctttct tttgcttatc cgttgtaagc
    45601 atcaatgatg atctgtttca attcagagat caatggttct ttcggattag ctgtcgtaca
    45661 ttggtcttcg taagctaatt cagccatacg gtctacggtt gtatctagtg tttcttgtgt
    45721 gactccttga gcttttagac tcatgttgat gccgacagat tttcctaatt cataaacagc
    45781 cgttgctaaa gcttcgacta attcctctgt tgtttcgcct ttcagaccta agaattttgc
    45841 gatatctgcg taatctgtat ctgcacggaa atagtcatat ttagggaaca tcgcatgttt
    45901 ttgtggatct tttgcattat agcggataat atgcggtaaa agaatcgcat ttgtccgtcc
    45961 atgagggatc ccgtattccc cgccgatttt atgtgcgact gagtgacaaa tacctaggaa
    46021 tgcgttagca aacgccatac cagccattgt agaagcgttg tgcatttttt cacgtgattc
    46081 catatcaggt gtttttactg atttttctaa atagtcaaag actaacttga ttgcttgtag
    46141 gctcaatcca cgtgtataat ctgaagccat gactgaaaca taggattcga ttgcatgtgt
    46201 taacacatcc atcccggtat ctgctgtgac agatgctgga actgacatga cgaattgagg
    46261 atcgacgatt gcgacatctg gcgtcaatgc ataatctgct aacggatatt tcacatgtgt
    46321 ttcactgtca gtgatgaccg caaatggcgt tacttcagaa ccagtccctg atgttgtcgg
    46381 gatacatacg aattgtgttt tttctggttt gtcgatctta tatgtccgtt tacggatatc
    46441 caagaatttt tgttttgcac caaagaaaga tgtgtctggg tgttcataga acatccacat
    46501 tcccttagct gcgtccattg cagaaccgcc tccaagagcg attactgtat ctggcttgaa
    46561 gtctgccatc agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc
    46621 tgagaagact tcgatcttca catcattttt acgtcgtgcc aatacttcac gaactgtatc
    46681 gcagtaaccg aattggacca taccagggtc acaaacgatc atgacacgtt caacgttttc
    46741 cattttctct aagtacaata gagagttctt ttcaaagaag atttttggcg gtaatttgaa
    46801 ccattgcatg ttatttctcc gtttcgctac agttttgaca ttgattaagt ttacagcaga
    46861 gacgttacga gaaacagagt tcttaccata agaaccgcat cccaaagtca atgacggaat
    46921 catttcgtta tagatatcac cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg
    46981 acaagctttc attcgaagac cgaatttgac atgcaactct tcgttttctg aatggataac
    47041 tgctgtatgt cctaatcctc caagattcaa cataccttca cacaaatcaa acgcatggtc
    47101 cgtgttgtta gctttgatca tggcaagaac tggagaaagt ttttctcttg atagcggata
    47161 atctgggcct acgccttcca attcagcaat cagcattttc gttccttttg gtacactgat
    47221 tcctgccaat tttgcgattt ccatcgcaga atgtccaacg attgatggat tgaccgcata
    47281 ttttccttcg ttcatcactg catcttctaa tttttgcaat tcatctggtt ttacgatata
    47341 aacttggtgt gcttggaatt ctgcttttac tgctgcatag atttctttat ccacgatcac
    47401 agcttgttca gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt
    47461 cactgcacgt ttaatttttg ctgttttttc aatataagct ggtacgttac ctggacctac
    47521 acctaaagct ggttttcctg tagagtaagc agacttgacc attccagctc cacctgtcgc
    47581 taagacgatc gcgatacctg gatggttcat tagcatagaa gtcgcttcga tcgaaggatg
    47641 ttcgatccat tggatacaat tttcaggagc tccggcagcg atagcagcat cacgtaccac
    47701 tcgtgctgct tctgcagaag atttttgggc acttggatgg aaagcaaaaa cgattggatt
    47761 tcttgttttc aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg
    47821 agttactccg caaaccacac cgactggttc agcaatttca atcaaccctt tttggacatc
    47881 ttcattgatc acaccaactg ttttatcatg tttgatatta tgccaaatat attcggacgc
    47941 atacatattt ttgattgctt tatcttcgta aacaccgcgg cctgtttcct ctacagccat
    48001 cttcgctaat ggcatatgtt gatccaaagc tgccattgcc atttcatgca cgatatggtc
    48061 tactttttct tgatcaaagc tttccatttc tttcaatgct gcatttgctt tttctgccaa
    48121 atcatcaatc attgcttgta cgtcaattgt cttcgttttt tctttttcta ttactttagc
    48181 catcattatc ctcctaatgg gtcatggtta tttgttaaat aattcacatg tatatcatac
    48241 aatatttatt tttctatgta aacacttttt tgtgattttt ttcacagtta tttttaaata
    48301 taaaattgct ttatttacag gtgtttgtat agaaaaatat ataaagttat ttattattat
    48361 ttatatgaca cacccgatta ttcaatattg agcaatatat cccatgctct tatctctttc
    48421 ttttttgaat aaaaaatgag gtaggacatc tttctgccct acctcgtctt taaataagct
    48481 ttgatactta tttttcttcc ttctcttctg tctcttccag cgtttctgtt gtttcagttt
    48541 ttacttcaac tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg
    48601 ttacgacagt ccctggtttg accgttttga ttgctgcacg atcgtactca aggaagatcc
    48661 cttcgcagtc taaaacaact gtccgtttgt cattgtcgat ttctgaaaca actccatgta
    48721 atccgccgat cgttacgact gaatcacctg gtttcatgct atctaacaag ttttgacgtt
    48781 cttgctgttg tttcttttgc gaacgtgaca taaaaaacca cattgctaat agcgcgataa
    48841 acatcacaag cattggtaat gttcccattt ttttcacctc actaattctg tatctacaaa
    48901 aaacacttta gcagaaaaat gctgataaca ctagtttttt acttaatttt taaaagattt
    48961 tcctttagaa gctttttgcg ttttctttat tgaacccgta ttcttcaaaa aaggcttctc
    49021 taaattcaag caaattatca tccatgattg cttgacgtac ttgtttcatt agattcaaca
    49081 agaagtataa attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca catttgatca
    49141 aatggcggat ataggcacgt gtatagtttt tacatgtata acaatcacat ttctcatcaa
    49201 ttggacggaa atcatgggca tatttcgcat ttttcactac tagacggcct tgagaagtca
    49261 tacatgtgcc atttcgagcg attcgagtag gcaagacaca gtcaaacatg tctactccac
    49321 ggataacacc atcgatcaat gaatcagcag caccgacacc catcaagtaa cgtggtttgt
    49381 tttcggggat caacggtgta gtgaattcta atacacgatt catttctgat ttaggttccc
    49441 cgacagataa cccgccgatt gagtaaccag ggaaatccat cccgactaga tctttcgcgc
    49501 tttgacgacg aagatcttca aaccctgctc cttggatgat tccaaataat ccttgacggt
    49561 ctggattagc atgagctttc aatcctcgtt ctgcccatcg agacgtacgt tctactgatt
    49621 ttttcacgta gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc atcatgatat
    49681 cagagcccaa tttattttgg atgttgattg ctttttctgg tgataaaaac atacgcgaac
    49741 cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat attccgcata tcacttaatg
    49801 aaaaaacttg aaagccgcct gaatctgtta aaatcggttg atcccagttc ataaatttat
    49861 gcagtccgcc agcttcttct actaagtctt caccagggcg tagccataaa tgatatgtat
    49921 tacttaaaat aacgccagca cccatttctt tcaactcttc tggggacatt gtttttactg
    49981 tagctaatgt tcctactggc atgaacattg gtgtggggaa agttccgtgt ggcgtgatca
    50041 attcccctaa acgcgcgcca gtatgttttt ctttcttgat taaacgatag cgaatggcag
    50101 gttgcgtcat tcatttccct actttctata taaaatatct tgacaactcc caccatcata
    50161 atcgtttcaa gtgctttttg caagttccct cggcttcctt ctactttatt taatattctt
    50221 ttttttatcc tttttttatg aataacaaaa aaaagaaaaa cattatgaca gaaatatttt
    50281 ttgatacatg gtatattggt gttattaaag aaaaggaggg aaaaatcatg caatcttctg
    50341 cagaattaaa acgccaagca aaagagagtc taaaaggacg ttggggacaa gctgttttac
    50401 taaatctaat ccctacattg attggtattg ctactattct tcttattatg attcctatta
    50461 tcttgtttgc caccaccata tatgatggat cagcgagcgt ctcagaagcg acaacttcag
    50521 gaggcggtag ttcaggaggc ggtggcggaa ttatttccag catcattagc gctttgttca
    50581 tgagcggtat ttcttggact tatcttgata ttttacgtgg acaaaaagat cagatcgaac
    50641 cattcaaaga tgcatttcgt ggattcaaag gtttgtttat cggcggagtg atcagcttag
    50701 ctgtgctgat ctcgatcttt acaacattat ggggtttgct attagttatt cctggaatca
    50761 ttaaaagcta ttcttattct caaagctatt ttatttacta cgatatcgtc caagaaacag
    50821 gacaaacacc taaagtactg gatacgatca ctgcaagtcg aaaattgatg gatggttata
    50881 aaggacaact tttctggtta gatctttcat ttatcggctg gcatatctta gcattagcaa
    50941 ctgctggtat tggttatcta tggctcagcc catacatttc tgcaacaaaa gcagcatttt
    51001 atgaacaatt gccaaaagat atttaaaaga gagtgtgact tgtgaccgag ggggtggcag
    51061 gagaacaccg tttatcgaga ggcagtaaaa gaaacattca gcccattagc aaaaaggagg
    51121 tgtgacaaca ttcatgtcac acctcctttt tgtatagttc cagtaaaaag aaaatcaact
    51181 tagaaacagt taatcattcc tcttcttctg cttccggtaa tcgatggaac ttgatcagtt
    51241 cattcaccgc gattggtacg atagataaaa tccctaccaa gaaccagtga ttcaaactga
    51301 tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag
    51361 ctaatgccag taacgccatt tctaccaaag ctttattggt cgctaaattg actttgaaga
    51421 tcgattggct gctgcgtaca ttgaacacat gcagaatcga tgaataagct aagatcaaga
    51481 atgcaacagt ttgaccgaca tgctgacttg cgtctaccca tgggctgata gtatcgacat
    51541 aactacctaa ataataacca aacaaggtta cgatggtgaa gacccctgca tttatggcaa
    51601 ttttttgcaa taaacctcga gcaaagatcc cttcattttt cggaatgggt ggctgatcca
    51661 tgatgccgtg ttccgctggc tctcttccta aagcaaatcc tggaagtcca tcagcaacta
    51721 cattgacaaa cagtagttgc acagctgtaa atggtgctcc ccatcctaac agcatcgcaa
    51781 tcaaaacaat aaagatctcc gaaatattac agcttagcaa aaagttgata gctttacgaa
    51841 tattctgata aaccgaacgt ccttgactga cagcgtctac gatcgtcgca aagttgtcat
    51901 ccgtcaaaat catatctgcg gcactttgcg ccacatctgt tcctgtgatc cccatggcac
    51961 acccaacgtt acttgctttc aacgctggtg catcattcac accatcccca gtcatcgcta
    52021 cgactgcacc cgtacgttgc caagatttga cgatccggat cttgtcttct ggcgttaccc
    52081 gagcatagac agagagagct ttgacacgcg catctaattc ttcgtctgat aattgatgca
    52141 gttctgatcc tgaaagtgct tctgacggat cattcaagat acctaattct ttcgctatgg
    52201 cactggcagt caccacatga tctccagtga tcatgactgt tttgatgcca gcctttttcg
    52261 ctcgcttgat tgctccttta ctttctggac gtggtggatc gatcattccg atcagaccaa
    52321 tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc ttgcggttct tcgtcataag
    52381 tagcataacc aacagcaatc actctcagtg cccgtttacc aaagcgatca ttaacgatcg
    52441 ccgcttgttc tacatcgcct gaactaaagc gcggagcaag aacatcgaat gctcctttgg
    52501 tgattgagat gtatttttcg cccatttggt gaactgttgt catcattttt cgttcagaat
    52561 caaaaggaag ctcgtttact cgtggatatt tttcttctaa ttctgccttt gtatgataat
    52621 tctcttcaac agcccttacg atggctactt ctgttggatt acctttgata acaaggtcgt
    52681 catcttcttg ctcgaccgtc acatctgtac atagtgcagc cattttcaat atttccatcg
    52741 cgctgttcgt cattgaatct tcgatattcg ttacttcatc ttctttgctc catacccggc
    52801 ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc tgagcagata acattagcgg
    52861 ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc gccatcttct
    52921 gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt acagctgcaa
    52981 cagccaagga aatcgaagtc atgaacatct caaggagtgg ttctccttgc agttcaccaa
    53041 tcacaaatac taaagccgca gccgctaaag cgatcatact gattcgtttg cctagctgat
    53101 tcaatcggac ctgtaaaggt gttttttgca tagtattttc gttcaaaaga tccgcgatct
    53161 tacccatttc tgtagccatg ccaatggcag tgacgatcgc tttcccacga cctgcagcaa
    53221 ccgtacatcc tttaaatacc atattaagtt ggtcacctaa tgactcatct tcttcagcaa
    53281 taaatgctgc atctttttca actgcttcac tttctccagt caacgccgat tcttctacac
    53341 gtaattgtga tgcttcgatc aaacgggcat cggcagaaat agcatcaccg gactctaaaa
    53401 caagaatatc acccactacc agttgctctg actcaacagt ggtcactttg ccgtctcgta
    53461 tgacagttgc cgtttgttta ttcatatctt gcaaggattc cagtgctttt tctgcgtttc
    53521 cttcttggac aatcgccaat accgagttga tcacaacgat agcgatgatc agcagtcctt
    53581 caaataaatc cccatgttct gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa
    53641 gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc atcgattctt
    53701 tcggcgcctc atcgaattta ttcaatccct gcttttttcg ccgttcatcc acttcttgtg
    53761 aagacaaccc agtatctgga tctgcttctg ttaccttctt tacttcttct attgtctgct
    53821 tgtattcttc catcctattc ctcctttaaa atcttcgtac aaaaaaaggt acttatttta
    53881 ctataacatc ctttttttat ttacttcaaa taaagggctt atcgattatt tgttaagaaa
    53941 gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa aggatgctat tatatagatg
    54001 taaggaagga ggataccatc aaatagacag aagaatttct cgatagaaca gtgtaagcaa
    54061 acaaagttgt tagaagtaca ggtttcaccc agacatgcgt gtagagtaca aacctataaa
    54121 tagtgaatgc gtatggatgt ttggatcacg aagtatccga aacaagaagt aatgcgatac
    54181 gtaacaattg aataacgtaa acgtctgaaa attgaatagg aacaagagat gtttcaaatg
    54241 ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac accgtgcttt
    54301 gcatgtatga tggaaagtta caatcatttc tgtttatttg aatccttccg aggtacaaga
    54361 aagccgagct tatcgagagc tcggcttttt ctttgtcata aaatctttgt aaagcttttt
    54421 tgttattcaa aactagcggc ttttcataac cttatttcac gaacatcgca tctccaaaac
    54481 taaagaaacg atagcgctct tcaatcgcat gctggtaagc agataaagtc aattctcttc
    54541 ctgcaaaggc actaactaac atgactaaag tcgattttgg caaatggaaa ttagttgaga
    54601 aagcttctac cactttgaat tcatagccag gcgtaatgaa aatatcagtc catccgctgt
    54661 ctgctttgat ttctccatta aatttcgttc caatcgtttc taatgttcga attgaagttg
    54721 ttccgacagc cacgattttc ccgccatttt tacgtacttg gttcaatcgc tcagccgctt
    54781 cttccgtcaa acggtagaat tcactatgca tatggtgttc ttcaatttta tctacgctga
    54841 caggtcggaa tgtacctaac cctacatgta gtgtcagata gactaattca acaccctttg
    54901 cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc agctgttggc gctgcagcag
    54961 aaccattttc tttcgcatag acagtttgat agcgatctgg gtcttccaga cgttctttga
    55021 tatatggagg caatggcatt tctcccaatg attcaagaat ctctagaaaa attccttcat
    55081 actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc tttcactact gcttgcaatc
    55141 tgccatcacc aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg
    55201 tctcccacgt atctccttgt gtattcgtta gaagaaggac ttctaaatgc gcaccagtat
    55261 ctggtttttc tccatataaa cgagcaggca gaactcgtgt attattcata actagcgcat
    55321 caccaggctg taactcttca atgatttcat ggaaatgttt atcttccatc tctccagttt
    55381 tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta
    55441 attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca ctcactttct
    55501 atcatgtgct tcatcattct atcatgtttt tcgttctgtt ggcaaaatca gcactttttc
    55561 acttctattt tttgttttcc taaataaaaa aactacccta tttgtatttt ttcattgttt
    55621 taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg gcaccatgat
    55681 tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa
    55741 tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa gcggaaaaac
    55801 gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa ttttgattga
    55861 tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg gctatgttat
    55921 ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa
    55981 attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga ttgatcttgt
    56041 ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg gtcagcagca
    56101 gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga tggacgagcc
    56161 attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca aagatttaca
    56221 agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag cgattaaact
    56281 agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc aatttgatac accagaaaat
    56341 attttacgtc atcctgcaaa cgatttcgta gaagagttga ttggagaaga tcgcctacta
    56401 caagccaagc caaatgcgac aagagtcggc gaagttatgc taaatacagc aatcacgatt
    56461 acaccggaaa aatcattaca agaagcaatc aaactgatga gggaaaaacg tgtagatacg
    56521 ttgttagtta ccgataattc gaatgtatta aaaggattta tcgatgtaga aacgttgaat
    56581 aaaaaacgag gaaaagtatc cagtgtcggc gacattttga ataaacatgt tttttacgta
    56641 aaagaatccg cctatcttcg tgataccttg caacgcattt taaaacgcgg gctgaaatac
    56701 gtgccggtcg ttgatgaaca gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg
    56761 gatatcgtct acgatgtgat ttggggagaa gatcccgcaa ctactgatgt tgcagagtca
    56821 gctactcctg aagaatctaa ggaggtctga cctatgcagc agttttttca tcagcacggt
    56881 tcagaaattc tctcaaaagg ttttgaacat ctttatattt caggacttgc tttgatcatt
    56941 ggtatcatca ttgctgtacc gcttggtgtc ttactgacac gttttccaaa agctgccacg
    57001 attgtgattg gcttaaccag tgccttgcag acaatccctt ctcttgcttt attggcattg
    57061 atgatcccgc tatttggcgt aggtaaatta cctgcgatta tcgcactctt tatttattca
    57121 ttattgccga ttttacgtaa tacgtacatt gggatgaaaa atgtcgattg gaattatcgg
    57181 gatgtggcaa aaggcatggg gatgactgag ttccaatcca ttttttccgt tgaattgccc
    57241 attgctatgc caacgatcat ggctggtatt cgtttagctg ccgtttacgt gattgcatgg
    57301 gctacacttg cctcttatat cggcgctggt ggtttaggcg atttgatttt tagtggactg
    57361 aataattacc aaccagatct cattttggca ggtacgatcc ctgtaacaat tttagcacta
    57421 ttggctgatt ggcttctagg tctcttggaa caccggctga caccgatcgc acttagagag
    57481 gagaatgaag aatgaaacgt atactaaaaa tgaccatggt tcttttctct cttcttatac
    57541 ttgcgagttg ttccttccct ggtcttgcca gcaatacaga tgaagacacc atttcgatca
    57601 caggtggaat cacttcagaa gcacagatct tagcaagtct agttgccgga atggttgaac
    57661 attatacaga taaaaatacc gcaatcatca acaatctggc tactacaact atcaaccatc
    57721 aagcgatgat gaacggagat gcttcaattt cagcggctcg ctacacagga acagatttaa
    57781 cgactacatt gaaccttcct cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag
    57841 atgaatttga gaaaagatac ggtcaaacgt ggttcccgtc ctatagtttt gaaaatacgt
    57901 atgtctttct tgttcgaaaa gacaccgcac aaaaatacca tctatctaaa gtcagcgatc
    57961 tgaaaaatgt agcggatgaa ttagtagctg gtgtagatac ttcttggatc aatcgtaaag
    58021 gagacggtta tgacggcttc caagaaactt atggcttctc cttcaattcg atattaccta
    58081 tgcagattgg acttgtttat gacgcagtcg aagcaggaaa aatggatatt gttcttggct
    58141 actctacgga tggacggatc gccagttatg atctagtcat gttggaagat gatcgtcaat
    58201 tcttcccacc atatgatgct gctccagttg tagacaacaa gatattagct gatacgcctc
    58261 atttaaaaga agccttaaat aagttaggtc ataccatcag tacggaaaaa atgcaggaat
    58321 tgaactacga agcagataat aatctagtgg aaccatcagt agtagcacaa cgtttcttac
    58381 aagaaaacca ctattttgaa gggaagtgaa gcaattggat aaaatgaatg tctttcaaca
    58441 atttttctat tatttccagg aaaatggctc ctatatcttt gcacaattta tccgtcactt
    58501 cctgatttct atctatggtg tcctctttgc agcagttgtc gggattccgg ttgggatcat
    58561 gatttctagg agacgtaaat tagcaaactg ggtgattcgt ttggcgaata tcatccagac
    58621 gatcccttct ctagccatga tctccatttt aatcattgga ttaggcctgg gagtcaatgt
    58681 cgtgattgtt actgtatttt tgtattccct cttaccgatc atcaagaata cgtataccgg
    58741 gatgatccaa gtagacaaaa acatccttga tgtcggcaaa ggcatgggga tgactgctag
    58801 acaacgacta ttcatggtag agttaccact ctctgtttct gttatcatgg caggcataag
    58861 aaacgcgtta gttgttgcca tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg
    58921 tcttggagat atcattatcc gaggaaccaa tgcaacagag ggcacatcga ttattttagc
    58981 aggtgcattg ccaactgcat taatggcgat catcacggac tggcttttag gtattttgga
    59041 aagacggcta gatccagcca gccgtacatc acgctgatgt tattgtaaaa aacaaaagct
    59101 tctgcgacca tttgttggtc gcaaaggctt ttttagttct gtcattttta aggcaataat
    59161 ccactctgat tcttccctcc ttatgggcaa agagttgctt ttcttagcta tttatccatt
    59221 ccgtatactc caacttcata ctgccaacgg gttcatatct gcatccaccg actaagtttt
    59281 tttcttatcc gctaaggcat tttgccaatc cattttccca tcactcgtca tggaattcat
    59341 accacatgat ctctaaatac tcgtctgctt gttctttgaa ttgggcatca attcttacat
    59401 cctttggcgt ggctttcatt taagcctttt ccatcagtcc tcgattgttc gatgccttca
    59461 gctgcatcat caaaactggt gactgccgta tcggtaactt cgatcatctt ttatacaatc
    59521 gggaacttgc catcctcatc cttggctaaa aacatatcta gccattccag cttctcgcca
    59581 tgggaggttt gcggttccta aaaccggtac acgattctta tggactgcat ccgtcgcaac
    59641 tagacttggc gatacgatga tccttcacca gaagagcacc cccgtaaacc aattgatcga
    59701 tatattgcca ataagaaaag acattggcgt aaaagatatc gatccctcgt gatgcattgc
    59761 cgttgatact gctattcatg gccgaaagcg ccacgacttt aacttcttcc gtttgagtga
    59821 cattcatgtt cggcaatgtc gctttggata cccgctctgc taaaggaacg acactggtat
    59881 tgtatttcgt atctgtatct tttccaaaag accatgccaa cgaatcctct gggaaccaat
    59941 aagacgactc cggttgattg tccattgctt ttttcaacat acttttggct gtttctcgat
    60001 aaacgacatt tccaattgtc ttcgcctcac tttggcgata atcgatggtc gttgccactg
    60061 ccaacactaa caacgatgct ccaattttac tgattttcct tgtcacactt cttcctcctt
    60121 gttttacata aacccgtcaa cattccattc ctaaactcaa acaatgaccc ttcaaaggtg
    60181 gagatatagg aacaaattaa agaaatcatg caataaatcc aagaaggttg gttttagaac
    60241 aaaaaaagag tctccttttt ggggagactc tttgttaact atctaaaaag gttggctctt
    60301 ctcacttcac tcataatgtg tccaggcaga ataaatttct acaactttat tttcctcatc
    60361 aactttgtaa accactcgat gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt
    60421 aggtctcagc ttttcaaaag actgcgtggg catatatgga tcttccttca atgtctgaat
    60481 cacttcttca aattgtttct ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc
    60541 agagtttttg attgcgactg tatagttact catcaaagag cttcccaatc aatatcatcg
    60601 atattcgtaa agctactgtc gtctttttcg cggtctcgta ctttatctaa agtacctgtt
    60661 tgctcaagaa aaagggtttc ttgaatcgct ctccagtctt ccagcccaat caatacagca
    60721 ttgtttccac tacgatcact aataatctcg atttcagtat gattttcatt tacctctttc
    60781 aataactgat aaaaattttt acgagcagtg ctaggattca tcgttgtatt agccattgtc
    60841 tctcctcctt tttctgatat aattatagcg tacttgatta agtacgtcaa aaaaatacaa
    60901 taaagcactt aaattcagca aacttttcct cttttttaca tagagaaaaa gacccctttt
    60961 tagggaggat ctttgtccac gctcacttct tactccttta cgactagcgg tagccaaacg
    61021 taaagtaatt ggtaacaacg tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc
    61081 tatgcaacaa caccaataca cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg
    61141 aataacctga ggatacgcac gaaagatttg taacggttat tcaaaactga ttttggccgt
    61201 caagtcttct actgcctcaa aaatgattgc ctctttcgtc tcctgtcatt catgttcact
    61261 atcgatttga tacttgctta gaccgttggg tttgtgaatc accaattaaa aatggcactg
    61321 agttatggta acaaaaaatg gtcacttgac gaggctgaga ttcctactta tgaagacaca
    61381 tggcacttaa gagtcgaggt aaaggaagaa gctgaaaata taggaggctg tacattattt
    61441 ggcaaaggat tcggaaatta tgatcaagac atcaaaatca aaatgtttta cagctaaata
    61501 ggaagaataa taacatttca tacgctgttc ccatctttaa tggcttatag agggcacctt
    61561 tagctgagaa taagacaaaa cagaccctct agcttcaatt aagagctaga gggtctgttt
    61621 ttgattttaa aacttggttt atttcttcaa atggtagcta taggtgctga caacgatatt
    61681 ttcacgccaa gacaatcgca atcttaaccg caaacttgtt tggttatgga gaatattctg
    61741 ccagcgtctg tttggtacaa attgaggaat caacacagta gttgtatagt tttgtttcgc
    61801 tgcattttta cttacgatat ctacataacg gataattgga ttttcaatcg agcgataaga
    61861 agagtggacg attgaaaaac ggacatctgg gaaatgcttt ttaaattctg cttcgatttc
    61921 tttttctttt tcgacatctt cgtctagcga aacatgcatc gcaacgacat aatcaccgat
    61981 cgaacgtgcg taattcaacg caccgatatt tacccgtgtc acattcccga ctaatacaag
    62041 aacggtattc ccgtcataat catgtagttt tgcatcattt tctaaacgaa gctgttctgc
    62101 tactttttta taatgatcat ggatcttata aaaaatgaac ataacgattg gcataatgat
    62161 gaagaaaggc caaatgtccc ctaaccgata gacaaacaaa atcgcaatga ttgcatatga
    62221 gatgaacgca cccgtaatat tggcaatcga tttgcttaac catctttttc cttctttttt
    62281 ccatttgacg accatccctg tttgagataa agcaaatgga atgaacacgc cgatagagta
    62341 taacggaatc aagcgttctg ttgagccatg gaaaataaac agcaatacga tcgaacctag
    62401 cgctagtgtg ataatcccat tggaatagcc tagtcgatcc ccacgatctt gatacatgtg
    62461 aggcatgaat ttatcttttg ccagattgta tgcaagaact ggaaaagcag aaaaaccggt
    62521 gttagcagca actgctaaaa tcagcgctgt agcaaattga aggacgtaat acaaaatacc
    62581 atgtccaaag acagctttac caatttgaga caaaacagtg acttcttttt caggaacgat
    62641 cccataccaa tagttgataa atgtgatccc cacgaagaag aaacctaaga tcaatgccat
    62701 catcgtcaat gtagcagcgg cattttttgc tcttggcttc ttaaagaatg gtacagcatt
    62761 actaatcgcc tccaccccag ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa
    62821 agcgatagaa acacccggca cagccgctcc tggaagtgca gtagcttgca atggcacagc
    62881 acctgtcacg atattatata aaccaacaac gatcagtaac gagatcacaa ggataaatgt
    62941 gtacaccgga aataatagga acgatgcaga ttccctcagc ccacgaagat tcagcatcat
    63001 cagtagcaaa acgattgtca cagaaatagc gacttgatga ccgtagagtg ctggcaccgc
    63061 tgatgtgatc gcttcagcac ctgctgagac agatactgct actgtcaaca tgtagtcgat
    63121 caataaagag ccaccggaaa tcaagccagc attctttcca agattctcac tgctgacgac
    63181 atatgctccc ccgccatgag gataagcatg gatgatttga cgatatgata gcgttaatga
    63241 aatcaataaa ataataacaa aagcggcaat gggtagcgaa taccagatgg ctgcagcaga
    63301 caaagcgact aaaacgacaa cgatttgttc cgtaccatat gcaatagatg ataacgcatc
    63361 agacgacaat aatgccaaag cagcaaatcg ggttaatttg tgctcatcat tttcagcaga
    63421 ttttaaaggc ttacctacta acaacctttt taagtaatcc aatttctaaa ctcctttaca
    63481 cacaatttta cttcaagttt tatagtcgaa aatttatagt aaacaagatg ttatagtacg
    63541 ctcactgacc ttaaaagtca ataaacttta agaaaataat tggaaaaaaa cagaaaaata
    63601 tcaagcgctt tttcaaacgc tttaatacag caaaaatcta acattttttt atgggctttt
    63661 tctttgaatt ctataataaa aggatagaac ttgacttaga aaatcaagtt ctatcctttt
    63721 tgatatctta acttttggaa gagagatgta aggctgcttt ctacccatca ttcttactac
    63781 tcgaagcata gcgcagttgt cacaacctct tgataaacgg tgtttaaaag cttgcatctg
    63841 cggtaataag cccaaccaaa ttaaaaacat aagaagctgt ttttatttgg tcgttcttat
    63901 tacttgatgc agagcagctt ttttacaacc tcttggtaca cggtgttcaa tcagctgacc
    63961 ctcggtatta gctcaaaaaa acggcaaaat atgaggaact atttttccat ttttttctct
    64021 aatactcagg tcaaaacact gatttcacaa cctctttttt taatgaaaaa tatggctctc
    64081 atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg aacggataga ttgccgttcc
    64141 tacgattcca aagacaacaa acaaaccgat catgatccag attgctttcg tagcatttgg
    64201 ccaataaatt cctcctgcag attcccgtaa caagtttacc gcatgagtaa atggcaagaa
    64261 cggattgatc atttggaaga atttccctga gacttggatc ggatagttcc cgcctccgcc
    64321 agagatcgac agtactaaga tgataatagc gatcccttta ccgacattat cgaataatgc
    64381 agccaagaca tagacgatca tcatgaacgc taaggcgatc aagacagcaa atgacacgct
    64441 gtagaccgga ttttggacat ctactcctag taagaaaata ttccctaatg taacgatcaa
    64501 gctttgcgca atcgccattg tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt
    64561 cgtaaaattg tttcggtctt tctttcctaa atagtattct gtcgtagcaa cacttgaaag
    64621 aagaacagcg ccgacccaca gacatagtgc agtataaaac ggggtgctag ctgaaccgtt
    64681 atttgcgatt ggataaagcg cattggtttg tagctcaaca ggttgtgtaa agaagtcact
    64741 ttcttttgtt gcgtctagtt tcaataattt gatgacttcg cctagatcga cttcttgttc
    64801 gccttttcgg atcgcgtctg ctgctttttg gataccagct ttgatatttg gccagtcatt
    64861 gttgatcaag tccgcagcag cgttcacagc tgtttcgact tctggcattt tttcatttac
    64921 tgttgtcaat gtaccggtga tttcattttt tagtgctggc caatcatttt ggataaaatc
    64981 agcggccaat cctaattttt gctgaatgat cggtaattca ttgttgtata ggtctgcccc
    65041 ttcattgatg ccattgacga tggtatccat atttccattc agcattgtat tggcatcatg
    65101 gatttcttgt ccaattgcag gcatttcttt ttgatatttt tctaaaattg ccactgcatt
    65161 cgttacagtt gctttcgttg agctgagaag cgaatcaaag tcgatctggt ccgctttgct
    65221 cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg tctagtagag aagtgacagt
    65281 ggaactgatt tgacctgtat tgataccagc aatgatgcca ttgatattat taacaacggc
    65341 atcaatttga tctagcaatt catcaatagc cttcgtgtct tcattttgaa cagctgtgtt
    65401 caaagcagat aggcggtttt gcagattgct tagcaagggt tttaattgat ttagctgatc
    65461 aataattggc tgtaatttcg tatttccagc acttgtttgc aatttagtca ggaactcgac
    65521 cagtttatcg attgcatttt gttgttgaga tattccgtca ataaagccgt tcaatacatt
    65581 gctgatgttt ttcttttcat catcagtcag cttaccatct tcaatagctg ttcttacagt
    65641 atctattaac gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg taacatctac
    65701 actgtttgtg atgcttggta tcgcatcttt cagctgagag gcacctgatt ttgtttggtt
    65761 tgctagatca cttgcctgat tttctaaatt tttcacatct ggcaagatat cttgtacttg
    65821 ttggatgatc gtcaagcctt gttttgcttc atcgattcct tggttcattg tctcttggat
    65881 cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg gcattttgga tctctgggat
    65941 tttttcttgc aaatcaagga tgatttttgc ttgatctttc aactcaggca ttttgtcatt
    66001 caatgcaacg actttttcac ccatctcatc tactttaggg atgtaatcca cgaattcatt
    66061 tgctttattc agtttttcct tgatttcagg tagttgactt tgtaattcga ggacttgttt
    66121 tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat aaaatcatat ctttaacttt
    66181 attgatactt actaggttcg tatcgatgtc gtaacctatc tcattgaata ctttcaataa
    66241 agtcgaactg gctgttttga taaattcatt tgtgatttgc gattggatcg aacttgctcc
    66301 tttatcggtg attttcggtg caatcgcatt gattttttca tttaccgtat attcgatttt
    66361 tggtttcttg atatcaccac tagtaaaact cagcagatct tctgagaaat cctttggcaa
    66421 gtaaatacct gcgtaatact ttccggaacg gactccgtct tctaattgtt cttttgaatc
    66481 aacgaattgc catcctaatt gtttattttt atgaagcgat tcgatgactt gctccccaat
    66541 ggccacttct ttaccttgaa actctgctgg cttgtctgca ctgtaaacag caattggcag
    66601 ctctccagta tttccatatg gatcccataa tgctttgatg ttaaaccacg catatagcga
    66661 agggatgatc atgatcgcga tgatcagaag tgttgcaatc gggtttttga aaatccgttt
    66721 ccagtcaagc aaatataatc gaagcgtatt tttgattgac tgcatagttc tcccccttct
    66781 tcttttttca gctgaattac tttaccatgt atttcatact tttttcaatc ggcttttttc
    66841 aaaaaagtcg aatttttgga catatcacgg caattgtctc attgtaatct cttacatatt
    66901 gagaaataat taaggattac agcctttact ttatcatgaa ataaaaggga aagataattt
    66961 tatgccaaag tttttatcta ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt
    67021 tgcccctaca aaaatgtttc agcttttttc gttttttatt cctgtatttc ttatttttcg
    67081 aatgacagga tgattttccc agcaggagga tgtccttcaa ctagctgatg tgcttggatg
    67141 acatgcttaa gtgaagcaga cagattcatt gaaataaaaa catgaaaggc tcctttttgg
    67201 taggctttga ttatgccagc aaaggcttct gcatctagat attcctttct tggaacaaac
    67261 gattcataaa agccttcctt tttttgccgc aaatctagat ctgggaggtc atttaatgca
    67321 acatacctgc cgcctggttt catgatctgg atgccggttt ctcctttgat acttcctttc
    67381 gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca gacctggatt tgttttatca
    67441 taagcagcaa atgctgaagc tcctaatttt tttacctttt cctcattttt actggatgca
    67501 ctggttaaga ttcggattcc cttttcatgg agaagctgga tcagacttga cccgaccgca
    67561 cctgatgctc ctagtatcat tacagtatct gtcggctgga tttccgtcaa atggttgatt
    67621 agattataag ccgtaattcc tggcgtgacc attccggctg cttcttccca actcatttta
    67681 tctggaattt ttgctacttt tgctgatggc aacacgactt cttctccgta agtcccgccg
    67741 actgcatgga cagctactcg atcacctaca tgaacagttg tgacatctga accaatctct
    67801 gtcacaatac cagcgccatc attacctggt acataaggga atttcaatgt tcgtatctct
    67861 ttcatttctc ctaaacgcaa ggcaacatca taaggattca cactgaatgc ttttatttct
    67921 acacgaacat gtccagaacc gacttctctg ggatgagcat cgatttcttc aaaaacatct
    67981 tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg tcaccatctc cctttttctt
    68041 taagtttacg tttatgcctt tattttgaca aaaaaaatgt cttttcgttt tggcgcatga
    68101 ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta aattatttat ctaaaagcgt
    68161 aaagattacg atttacgcta ataaaaaata attggaggaa tttgttgatg gctaaaaaat
    68221 caaagattgc caaagcaaaa aaacagcaag aaacgattga acgctatgct gctttacgcc
    68281 aggaattaaa agcgcaaaaa gattacgaag gcttgcgtaa acttccttta gatgctcgtc
    68341 cagaacgatt gaaaaatcgt gatttaatcg atggacgccc tcgcggatac atgcgtaaat
    68401 tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa aggtttgatt ccaggagtaa
    68461 aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa ggaggagggc gcatggacca
    68521 ctccttggat catcaaaaaa gatacgttcg cttcttctag cagaaatgct ggtactagaa
    68581 gcagacgtat ctttttttac gcacagctgg tcctttttta tatgtaaagg ctgcatggtt
    68641 acttcgctat atttatttag taacaggagt cagtgcttcg atacccacac taattttcaa
    68701 tgctcggtca acttctgcca taatgggtcg atccaaatgg cagacctttt ctttcaatct
    68761 tgatttatcg atcgttctga tctgctccaa caggataacg gagtctcttt ctattcctgt
    68821 tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt ttagccattt ttgccgttat
    68881 ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga ttattttgaa taaccaaaac
    68941 tgggcgtgtt ccgccttgct ccgatcccac gactggagaa agatcagcaa aataaatatc
    69001 tcctcttttt accataggat cctccttatt cttgatattc tcttggtatc cgctgtccta
    69061 atccgcaagc cacttcatag tggatcgtac ctaattgatc tgctatatcc tgcatagtga
    69121 tttctttgtc tgcatttttg ccgataagcg tcacttttgt tccgacagga aattcttgag
    69181 gcaatcggat catcagttga tccatacata cacgcccaat cgtttcacag tagttccctt
    69241 ctaccaatag agaaaaaccc tgcatctttc gtggccagcc atctgcataa ccgatcggta
    69301 tcgttccgat ccactctgct tcaggagtga tatatgtttc tccatagccg attccttcac
    69361 ctgctgggag ttttttgact tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg
    69421 gataaacttc cggtagtgcg tgcccagagg gattaagtcc gtacattgct actccataac
    69481 gaatcatatt gccgattgtt tcatcatgcc ataaagccgt tgcactattg ctgacatgta
    69541 catatcgagg aagttcggga agttttttca gcacttctat aaagcgttct ttttgcagat
    69601 tccaatatgt atcatctgcc tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt
    69661 ctaatacacg actttctttg atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa
    69721 atccgatacg acccatccca gtgtctgctt ttagatgaat cgaaagaggc gtctctagct
    69781 tacttttctc taaaacagca gctgctgctt gcaaccattc ccgtgtacct gcagttactg
    69841 ataaatcatg agcaacagct acagtcagtg cgtcgtaagg aaccatattc aagataagga
    69901 tgggctgagt aaatccagct tctctcaact cgactccttc atctaaattt gaaacacaaa
    69961 agcccgaagc tccacctgca actgctgcct cagctgtttc aatagctcca tgaccgtatc
    70021 cgttcgcttt tactactgca aacaattctt ttccttgcgg aagtcttgca acttcatttg
    70081 ctacattttc agttatagct tttttatgta tcactgcttt cgttgggcgg tgccaagcga
    70141 caaccatcgt ttttccttct ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat
    70201 ttgtatgagt aatactgacc catacattgc cttcatgagg tgaacgcgtc acaacaggct
    70261 gaccattttc attttttaaa atctcgacat cttgaaaggt aacttttccg atcccagttc
    70321 cccaagcttt agagaaagcc tctttgcaag cgtaacgccc gcctaaaaat tctacttggc
    70381 gatgaaaagg caaggactgg aatagtttca tttcatcaga ggtcaagatt cgtgcaatga
    70441 attttggttt ttcttcgatc agccgagtga tcctaggaag ttcgactgca tcgatcccaa
    70501 ttccttttat catatgcttt tctcttttca ataaatttag aacactctca ttctaccaaa
    70561 ttataaccat tttgccacaa ataagtccac aaataacaaa aatgaaagtt tctcttaaaa
    70621 aagagaaact ttcattttat gtttaatcgt tatttgaacg gattacaaat ccgcgttttt
    70681 tatcattgtg acgtttaccg ccatcttttt ggctgttatt atttttgcta taaccgcctt
    70741 ttgatttgtt tccacgataa ttaccgccgt cacgacgatt acggttgttt ccgccaccac
    70801 gtttgccatt tttattgtat ccttttttct gcattggcaa tgggcgttct ggtgtgatct
    70861 tcactggcac tgcatcagct ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat
    70921 cttgtgcaga atattcttct aataatttat ctgctgtttg caggtattta tctagaccat
    70981 tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc taattgacct ttgaatgctt
    71041 ctttttcggt tggtggacgt aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat
    71101 gcaagtagtc catttcattt ggtgtaacga aagtcacaga cataccgcct tttccggcac
    71161 ggcctgtacg tccgatacgg tgaacgtagc tttctggatc ttgagggata tcatagttgt
    71221 acacatgagt gacacctgaa atatctaatc cacgtgcagc aacgtctgtc gctacaagga
    71281 tatctaaatg accgctctta aatgaacgca atacgctcat tcgtttttgt tgagacaaat
    71341 cgccatggat tccttctgca cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta
    71401 cacggcgttt tgtacgtccg aagacgattg ttaattcagg tgtttgaaca tcaaacaagc
    71461 gagtcatgat atcgaatttt tcgtattctt ttgcgcggac atagtattga tcgatcaagt
    71521 cagctgtcat ttctttcgct ttgatcttca catgatgtgg atttttcatg aatttaacgc
    71581 caatattctt gatagctggc ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag
    71641 gaacttgtga aatgattttt tcgatatctt ccaagaagcc catattcaac atttcatctg
    71701 cttcatccaa aactaatgtt tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt
    71761 caagcatacg tcctggtgta cccacaacga tatgtggacg atctttcaag ccacggatct
    71821 gacggccaat atctgctccg ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta
    71881 aacgatataa ttcttcttgt gtttggatag ctaattcacg tgttggtgcg ataactaaac
    71941 cttgtaattc gtgacgatct ggatcgattt tttctaacat tggtaatcca aatgcagctg
    72001 ttttccctgt acctgtctgt gcttgtccaa taacgtcttt tccagctaat gctagtggaa
    72061 tcgtttctgc ttggataggc gttgcttctt caaaaccagc gcgttcgact gatttcaaca
    72121 attctggtga taattctagt tctttaaatt tcaaatgaat cctcctatat ggttgttaac
    72181 tgaggatgta gcttttttgc taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt
    72241 ttgacagtgg ccacgcacgt ttttatccat tcttagcccc tgtggcacca ccgaaaaatc
    72301 actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg cgttcagctt tatcattatt
    72361 atgacatctg gcttcaacaa gttgtttcta tggcaaaacc agtccaattt ctcttcatct
    72421 ttcggcaata ggttattcta acatagtttt tcgttttctg caagtttaca gatttttatt
    72481 tcatttgctg aagttgttct accacttcaa ataagcccat gctgttgctg ccttttagca
    72541 taatactatc tgttgcttca agattttgtt tgatagcatc aatcaattta ttcttttctt
    72601 tttcaaaata gaacacaggt agttgggggt atttttggtt caattcttct tttagtgcat
    72661 gcatttgttc cccataaagg aaaatcatgg cgaatttctc aggatcgata tgttcactca
    72721 tttgcgcatg catatttaaa gactctttcc ctaattccag catatctgct aacacagcga
    72781 ttctgcgtcc ttttgttggc agatttttaa aagtatctaa taccagtccc atggctgttg
    72841 gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg
    72901 ttctgttttg agtgatagaa acatgggcga gcccttggcg tatctcaggt atggacagtc
    72961 caaagaagcg cccgatcgta tacgcaatca aagcattcgt tacattataa cttccaggca
    73021 gcgggatcgt aaatgtttct ccttctacta caaaatcggt ttgtgctttt ccttttgctg
    73081 tcacttccgc atgaacatcg ccatttccaa taccgaatgt tgtgactgtt tgtactaatg
    73141 ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac aacgagtaat ccgtctgata
    73201 caagtccgtc agtgatttcc attttggctt tagcgattcc ttctcgcgac ccaagatttt
    73261 ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt tggttgagcc ataagtgaaa
    73321 gttccgtgat ttcattggcg tgatccatgc ccatttccaa aacgatcttt tctgtatctt
    73381 ggggcatgtg caaaatcgtg taaggaagac caatattatt gttgtaattt ccttgcgtct
    73441 tatatgtttt gtatttttca gacaagacag acgcggttag atctttcgtg gtggttttac
    73501 cattgcttcc tgtaattgca acgacttctg cgcctacttt ttccaagtaa taggttgcca
    73561 attgctgcat ggctttcaaa ggatctgcta caggaataac tggaaaattt tttggagctt
    73621 gttcaacagg caagctccaa aatgaagcag atgctccgtt tgtgatcgct tgctggataa
    73681 attcatgtcc atctcgcgta ccagcaagag gaacgaataa acttccttcg gttatcttcc
    73741 ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga gcattcgctg cccacggctt
    73801 gtgcaatttc ttgtattgtt agattcattg agatctctcc taaaaaaggt tgtgaaaaaa
    73861 gcgtttggcc taaacggcgg gttcgcatga ctctcacttc caagtaaatt tctctattcg
    73921 tatttagaag ctagaatgaa cgcggttacg caatcactcc agcctcttct ttatttctct
    73981 ttattcattt ttttcaaaaa aagattgacg ctgatggtat cgattcattc ctaactggat
    74041 cagttcttca atcaaatcac cgtatttcaa gcccatattt tcccataaga gtgggtacat
    74101 actgaactcc gtaaatcctg gcatagagtt taattcattc aggaataatt catttttatt
    74161 tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct aacatcgtgt aagctaactt
    74221 cgcgtactct tgcgcttttt gataaacttc ttctggcact tcggctggaa tctgcatttc
    74281 gattttatta ttgatatatt ttgcttcata atcatagaat gctacgtctt ttacgacttc
    74341 accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc
    74401 ttcaattcct tgttcaacga ttgctcgaga atcatactga taggctgttg ctaaagcatt
    74461 ttgcagctct tctcggtttt ctgcttttgt aatgccgaca ctagaaccca tattcgccgg
    74521 tttgacaaac atcggataaa gcaaagaacc ttcacattga tcaaatactt ttttaggatt
    74581 ttctttccat tgattcttaa gtactggtac ataaggaact tgcggcacac cagcagcttg
    74641 taaaatatac ttggtcatga ttttatccat ggcacatgca ctggtcaata cgcctgcgcc
    74701 gacataaggc atattcaatg tctctaagaa gccttggatc gttccatctt ccccgtttgg
    74761 cccatgtaaa actggaaaaa cgatggctcc ttcttctttg atttcgcccg gattgatcac
    74821 ttttcctgta aagccttcct ctgtctgtcc acttgggtcc catgaaagat gcaagacatc
    74881 tttgctggca ggtttttctg ttagtaatgg acctttgacc cattgtcctt cttttgtaat
    74941 aaatacgagt tgaacttggt aataattata ataaatggca tttaaaactg aaaatgcgga
    75001 aagaatggac acttcatgct ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag
    75061 attgtcctcc tgtattctaa atcgttcagt gatttactct catattttag cacaaaacaa
    75121 aaataaacga ataacttatt tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat
    75181 ttattttttg tcagctagat tatttgtccc gttttttata aaaagttatg ataaagaaaa
    75241 ggagggatga tgatggcgca aacaaatcca cgcagccgct tggcagaaat cattcgagtg
    75301 ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa
    75361 aaagcctttg aagaactagg ccccaccttc atcaagatag gacagatgct ttctgttcga
    75421 gaagatttgc tttcttctgc cttcactcaa acattcaaaa cattacaaga tagtgtacct
    75481 agcgacacat tttcaacggt caaaaagacg atcgaaacag aactttctct ttcattatca
    75541 gatatattcg atgatttttc aaaatcgcct tttgcctctg cttctatggg gcaggctcat
    75601 cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc taacatcgcc
    75661 gaagagatcc gacttgatct tcaactattt gaacgagcga tccctttgat caaatacatt
    75721 cctgaaacta gcgtagttga tctaaaaggg gtactccaag aagtaaaacg ttccttgatc
    75781 aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac aattctatca aaagaacaat
    75841 ggctggaaag agatccgcag ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc
    75901 gtgatggaag aaatgtctgg taaaaacttg aatcatttaa tgaacgccga aaacaaaact
    75961 gaaactttta tcactggaat tcaaaacaag cagctgaaac aagaagttgc aaagctttta
    76021 gttgagaact ttatgaaaca ggtgtttgat gatggttttt tccatgctga tcctcatcca
    76081 gggaatctgc tattccatgt actaacaaaa gaagaacaga cacaagctag ccgaaaaact
    76141 gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc gagcctccac ttctgcagaa
    76201 gaccctgttg cgccatatac catcaactac atcgattttg gtatgatggg gcatctttct
    76261 gcaggactta ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa agatgcttat
    76321 cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg
    76381 ttccaccaag aactgacaag ttttcttgaa cagtattatg attctcctat tgatgaaatc
    76441 aacttgcaag aagtattcac acatgtcgtg acgatttgtc atcaaaataa tctgcagttc
    76501 gatcgtgata tcacgctttt gctgaaagca tttgggacat tagaaggggt gatccgcgtc
    76561 ctcgatcctg aagtttccct catggaagtc gcttcgcctt ttgctcaaca ttattttctc
    76621 actcatctgg atgtagaaga tacattgaaa caaagcggat tagacttatt ggaagggatg
    76681 aaggctgcgc ctaagatccc tcaacagctc caccatctat tagaaatgtg gacttcggga
    76741 caaggaaaag tcaatttgga attgaaaaag caagacaaat tattgagcag gatcgaaagt
    76801 atgatcaatc ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg gtcctccctg
    76861 cttgtccaag cagcacctgt agaaaacgca gaggtcgttt ccttattagg gatatttacg
    76921 tatgctattg ccgcattcgt cattattttt ttggcaatcg atgcattgat ccaaatgtat
    76981 aagaagagga aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa
    77041 ggggttttac ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag
    77101 gctacttctg aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa
    77161 gaacaatcaa aaagggaaca tggaacacat ttatcctgtt tccctgttcc ctttttgatg
    77221 tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg gtaaacctta
    77281 gtcaattgcc ttttttaacg cttgacgaaa agacatatga tctttctccg taaagggttt
    77341 tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac gttgttctag
    77401 aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca ctcgcacacc
    77461 cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt cattcttctt
    77521 aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt agacgatttg
    77581 gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga taaccacgtc
    77641 aagaccatag gattctccta attttatgac atcttctttt actggagaac catctccatc
    77701 aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt actaatccgc
    77761 tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca ctttctatcg
    77821 gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc atttcttttg
    77881 ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct ttattttgag
    77941 tagtatactt gacaacgatc cggcgatcca ttggaatagc tgtatcagtt tgataatatt
    78001 ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag acacctatcg
    78061 caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc aattgcatag
    78121 ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt gctgcaatca
    78181 agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg tacagtatgc
    78241 caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt agaaaagtaa
    78301 acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa acaaacacga
    78361 acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt tcatcgaata
    78421 acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat aaaacaagct
    78481 ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa cgcggataat
    78541 gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa
    78601 tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag atagctgcaa
    78661 ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga aaattgaccg
    78721 tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt gaaaaataaa
    78781 ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca ccaatcagca
    78841 gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg actaaaaata
    78901 gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc caattatgcg
    78961 agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta cttaagataa
    79021 agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac atgacaatgg
    79081 ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag gggaaataaa
    79141 ccaattttac agaatatatt cttgtaaaaa aaaatacaga aagaagcata agcttctttc
    79201 tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa aagtcttgcc
    79261 cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt gcctccaaaa
    79321 tcaattagga gctctgtaaa ccagagtcct tctcgacccg tcgcataaat ttactttaac
    79381 acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt tgaaatcctt
    79441 gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta taaacctcga
    79501 ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt gtttgcgcag
    79561 caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt actttattta
    79621 gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca gtgtaaccga
    79681 cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt tctttttgaa
    79741 tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga ccaatgatcg
    79801 gcacaaaaac ttccggccat acttcttgta tgactgccat cagctcatcc agttcatttt
    79861 ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa tgaccttgcg
    79921 cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccac ggtttcaaga
    79981 aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct actagacaga
    80041 tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt ttaatatctt
    80101 tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat aaaataagct
    80161 ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc ccaatcacta
    80221 ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg cccgctactg
    80281 aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac agaacaaata
    80341 tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg ctcgatcgaa
    80401 aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga ccaataatgg
    80461 gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat attccccaca
    80521 tcttttttca tttattaaaa ctgcctcctt
//
LOCUS       DANKBF010000004        72829 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000004 DANKBF010000000
VERSION     DANKBF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(168..1793)
                     /gene="groL"
                     /locus_tag="PWY89_000290"
     CDS             complement(168..1793)
                     /gene="groL"
                     /locus_tag="PWY89_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2065354.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(1844..2128)
                     /gene="groES"
                     /locus_tag="PWY89_000291"
     CDS             complement(1844..2128)
                     /gene="groES"
                     /locus_tag="PWY89_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2065355.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            2353..3015
                     /locus_tag="PWY89_000292"
     CDS             2353..3015
                     /locus_tag="PWY89_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2065356.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(3091..4383)
                     /locus_tag="PWY89_000293"
     CDS             complement(3091..4383)
                     /locus_tag="PWY89_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2065357.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            4553..5182
                     /locus_tag="PWY89_000294"
     CDS             4553..5182
                     /locus_tag="PWY89_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2065358.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(5285..6094)
                     /locus_tag="PWY89_000295"
     CDS             complement(5285..6094)
                     /locus_tag="PWY89_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2065359.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(6149..7018)
                     /locus_tag="PWY89_000296"
     CDS             complement(6149..7018)
                     /locus_tag="PWY89_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL2065360.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(7019..8335)
                     /locus_tag="PWY89_000297"
     CDS             complement(7019..8335)
                     /locus_tag="PWY89_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065361.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(8332..10167)
                     /gene="walK"
                     /locus_tag="PWY89_000298"
     CDS             complement(8332..10167)
                     /gene="walK"
                     /locus_tag="PWY89_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL2065362.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(10172..10876)
                     /locus_tag="PWY89_000299"
     CDS             complement(10172..10876)
                     /locus_tag="PWY89_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2065363.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(11066..11926)
                     /locus_tag="PWY89_000300"
     CDS             complement(11066..11926)
                     /locus_tag="PWY89_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2065364.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(11913..12482)
                     /locus_tag="PWY89_000301"
     CDS             complement(11913..12482)
                     /locus_tag="PWY89_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL2065365.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            12863..12935
                     /locus_tag="PWY89_000302"
     tRNA            12863..12935
                     /locus_tag="PWY89_000302"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12896..12898,aa:Lys,seq:ctt)
     gene            complement(13431..13817)
                     /locus_tag="PWY89_000303"
     CDS             complement(13431..13817)
                     /locus_tag="PWY89_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2065366.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(13814..14335)
                     /locus_tag="PWY89_000304"
     CDS             complement(13814..14335)
                     /locus_tag="PWY89_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2065367.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(14337..15371)
                     /gene="sppA"
                     /locus_tag="PWY89_000305"
     CDS             complement(14337..15371)
                     /gene="sppA"
                     /locus_tag="PWY89_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2065368.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(15404..16819)
                     /locus_tag="PWY89_000306"
     CDS             complement(15404..16819)
                     /locus_tag="PWY89_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDL2065369.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(16788..18941)
                     /locus_tag="PWY89_000307"
     CDS             complement(16788..18941)
                     /locus_tag="PWY89_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDL2065370.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            19199..19435
                     /locus_tag="PWY89_000308"
     CDS             19199..19435
                     /locus_tag="PWY89_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDL2065371.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            19454..19669
                     /locus_tag="PWY89_000309"
     CDS             19454..19669
                     /locus_tag="PWY89_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDL2065372.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(20372..21124)
                     /locus_tag="PWY89_000310"
     CDS             complement(20372..21124)
                     /locus_tag="PWY89_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2065373.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(21139..22386)
                     /locus_tag="PWY89_000311"
     CDS             complement(21139..22386)
                     /locus_tag="PWY89_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2065374.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(22462..22608)
                     /locus_tag="PWY89_000312"
     CDS             complement(22462..22608)
                     /locus_tag="PWY89_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL2065375.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(22712..23023)
                     /locus_tag="PWY89_000313"
     CDS             complement(22712..23023)
                     /locus_tag="PWY89_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2065376.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(23025..23222)
                     /locus_tag="PWY89_000314"
     CDS             complement(23025..23222)
                     /locus_tag="PWY89_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL2065377.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(23616..25343)
                     /locus_tag="PWY89_000315"
     CDS             complement(23616..25343)
                     /locus_tag="PWY89_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL2065378.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(25336..26529)
                     /locus_tag="PWY89_000316"
     CDS             complement(25336..26529)
                     /locus_tag="PWY89_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2065379.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(26716..27360)
                     /locus_tag="PWY89_000317"
     CDS             complement(26716..27360)
                     /locus_tag="PWY89_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2065380.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(27454..27588)
                     /locus_tag="PWY89_000318"
     CDS             complement(27454..27588)
                     /locus_tag="PWY89_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL2065381.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(27590..29722)
                     /gene="feoB"
                     /locus_tag="PWY89_000319"
     CDS             complement(27590..29722)
                     /gene="feoB"
                     /locus_tag="PWY89_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL2065382.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(29719..30192)
                     /locus_tag="PWY89_000320"
     CDS             complement(29719..30192)
                     /locus_tag="PWY89_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL2065383.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            30593..30817
                     /gene="nrdH"
                     /locus_tag="PWY89_000321"
     CDS             30593..30817
                     /gene="nrdH"
                     /locus_tag="PWY89_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL2065384.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            30976..33135
                     /gene="nrdE"
                     /locus_tag="PWY89_000322"
     CDS             30976..33135
                     /gene="nrdE"
                     /locus_tag="PWY89_000322"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL2065385.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            33185..34150
                     /gene="nrdF"
                     /locus_tag="PWY89_000323"
     CDS             33185..34150
                     /gene="nrdF"
                     /locus_tag="PWY89_000323"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL2065386.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(34239..35378)
                     /locus_tag="PWY89_000324"
     CDS             complement(34239..35378)
                     /locus_tag="PWY89_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2065387.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(35687..36400)
                     /locus_tag="PWY89_000325"
     CDS             complement(35687..36400)
                     /locus_tag="PWY89_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL2065388.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(36654..37355)
                     /gene="nagB"
                     /locus_tag="PWY89_000326"
     CDS             complement(36654..37355)
                     /gene="nagB"
                     /locus_tag="PWY89_000326"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2065389.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            37589..39121
                     /locus_tag="PWY89_000327"
     CDS             37589..39121
                     /locus_tag="PWY89_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2065390.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(39312..40514)
                     /locus_tag="PWY89_000328"
     CDS             complement(39312..40514)
                     /locus_tag="PWY89_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2065391.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(40596..41285)
                     /locus_tag="PWY89_000329"
     CDS             complement(40596..41285)
                     /locus_tag="PWY89_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2065392.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(41391..41759)
                     /gene="rplL"
                     /locus_tag="PWY89_000330"
     CDS             complement(41391..41759)
                     /gene="rplL"
                     /locus_tag="PWY89_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2065393.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(41812..42315)
                     /locus_tag="PWY89_000331"
     CDS             complement(41812..42315)
                     /locus_tag="PWY89_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2065394.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(42519..42646)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(42725..43414)
                     /gene="rplA"
                     /locus_tag="PWY89_000332"
     CDS             complement(42725..43414)
                     /gene="rplA"
                     /locus_tag="PWY89_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2065395.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(43518..43940)
                     /gene="rplK"
                     /locus_tag="PWY89_000333"
     CDS             complement(43518..43940)
                     /gene="rplK"
                     /locus_tag="PWY89_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2065396.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            44283..44951
                     /gene="sdaAB"
                     /locus_tag="PWY89_000334"
     CDS             44283..44951
                     /gene="sdaAB"
                     /locus_tag="PWY89_000334"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL2065397.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            44964..45836
                     /gene="sdaAA"
                     /locus_tag="PWY89_000335"
     CDS             44964..45836
                     /gene="sdaAA"
                     /locus_tag="PWY89_000335"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2065398.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(45927..46946)
                     /locus_tag="PWY89_000336"
     CDS             complement(45927..46946)
                     /locus_tag="PWY89_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2065399.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKY
                     TKLMD"
     gene            complement(47119..48024)
                     /locus_tag="PWY89_000337"
     CDS             complement(47119..48024)
                     /locus_tag="PWY89_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2065400.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(48083..48628)
                     /gene="nusG"
                     /locus_tag="PWY89_000338"
     CDS             complement(48083..48628)
                     /gene="nusG"
                     /locus_tag="PWY89_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2065401.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(48729..48899)
                     /gene="secE"
                     /locus_tag="PWY89_000339"
     CDS             complement(48729..48899)
                     /gene="secE"
                     /locus_tag="PWY89_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2065402.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(48923..49075)
                     /gene="rpmG"
                     /locus_tag="PWY89_000340"
     CDS             complement(48923..49075)
                     /gene="rpmG"
                     /locus_tag="PWY89_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2065403.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            49237..49881
                     /locus_tag="PWY89_000341"
     CDS             49237..49881
                     /locus_tag="PWY89_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2065404.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(49937..50440)
                     /locus_tag="PWY89_000342"
     CDS             complement(49937..50440)
                     /locus_tag="PWY89_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2065405.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(50453..50499)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            51037..52002
                     /locus_tag="PWY89_000343"
     CDS             51037..52002
                     /locus_tag="PWY89_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2065406.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(52101..53273)
                     /locus_tag="PWY89_000344"
     CDS             complement(52101..53273)
                     /locus_tag="PWY89_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065407.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(53260..54405)
                     /locus_tag="PWY89_000345"
     CDS             complement(53260..54405)
                     /locus_tag="PWY89_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065408.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(54419..55343)
                     /locus_tag="PWY89_000346"
                     /pseudo
     CDS             complement(54419..55343)
                     /locus_tag="PWY89_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            55460..56539
                     /locus_tag="PWY89_000347"
     CDS             55460..56539
                     /locus_tag="PWY89_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2065409.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            56571..57164
                     /locus_tag="PWY89_000348"
     CDS             56571..57164
                     /locus_tag="PWY89_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2065410.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(57274..57501)
                     /locus_tag="PWY89_000349"
     CDS             complement(57274..57501)
                     /locus_tag="PWY89_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2065411.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(57604..60192)
                     /locus_tag="PWY89_000350"
     CDS             complement(57604..60192)
                     /locus_tag="PWY89_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL2065412.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(60212..60871)
                     /locus_tag="PWY89_000351"
     CDS             complement(60212..60871)
                     /locus_tag="PWY89_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2065413.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(60920..61423)
                     /gene="trmL"
                     /locus_tag="PWY89_000352"
     CDS             complement(60920..61423)
                     /gene="trmL"
                     /locus_tag="PWY89_000352"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2065414.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(61644..62510)
                     /locus_tag="PWY89_000353"
     CDS             complement(61644..62510)
                     /locus_tag="PWY89_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2065415.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            62619..63260
                     /locus_tag="PWY89_000354"
     CDS             62619..63260
                     /locus_tag="PWY89_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL2065416.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(63322..64683)
                     /gene="mgtE"
                     /locus_tag="PWY89_000355"
     CDS             complement(63322..64683)
                     /gene="mgtE"
                     /locus_tag="PWY89_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL2065417.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(64701..65600)
                     /locus_tag="PWY89_000356"
     CDS             complement(64701..65600)
                     /locus_tag="PWY89_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2065418.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(65602..66399)
                     /locus_tag="PWY89_000357"
     CDS             complement(65602..66399)
                     /locus_tag="PWY89_000357"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL2065419.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(66377..67081)
                     /locus_tag="PWY89_000358"
     CDS             complement(66377..67081)
                     /locus_tag="PWY89_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL2065420.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            67212..67788
                     /locus_tag="PWY89_000359"
                     /pseudo
     CDS             67212..67788
                     /locus_tag="PWY89_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            67906..68583
                     /locus_tag="PWY89_000360"
     CDS             67906..68583
                     /locus_tag="PWY89_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2065421.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(68662..70470)
                     /gene="pepF"
                     /locus_tag="PWY89_000361"
     CDS             complement(68662..70470)
                     /gene="pepF"
                     /locus_tag="PWY89_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL2065422.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(70646..71644)
                     /locus_tag="PWY89_000362"
     CDS             complement(70646..71644)
                     /locus_tag="PWY89_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL2065423.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(71870..72523)
                     /locus_tag="PWY89_000363"
     CDS             complement(71870..72523)
                     /locus_tag="PWY89_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL2065424.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        21817 a        15028 c        12386 g        23598 t
ORIGIN      
        1 agcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca
       61 atttccgtga atttcttctt atttcttgga gttaaacgct tctgttccag cctcttttta
      121 tctgtctttc actaatgatc agttgtagat aagcaagttg ctttttctta catcataccg
      181 cccatcattg atggatccat tgcaggagct gctggcgctg ctggttctgg tttatctgca
      241 acgactgctt ctgtcgttaa taacaaggca gaaacggaag cggcattttg caaggcagaa
      301 cgtgttactt tagttgggtc tacgatacct gcttcaacca tgtttaccca ttctccagta
      361 gcagcattga aaccagtgcc tagttccacg ttcttcaatt tatcaacaat cactgatcct
      421 tcataaccag cgttttcagc gatttgacga attggttctt ctaaagcacg aacaacgatc
      481 ttgatacctg ttgccacgtc tccttctgct tctacagcag aaactttgct gataacgttc
      541 actagagctg taccaccacc tgagaccatt ccttcttcta ctgcggcacg tgtagcattc
      601 aatgcatctt caatgcgtaa ttttaattct ttcaattctg tttcagtagc tgcaccaact
      661 ttaacaacag ctacaccacc tgctaattta gctaagcgtt cttgtaattt ttcgcggtca
      721 aaatcagaag ttgtttcagc aatttggttt ttgatcaatt gcacacgagc ttcgattgct
      781 tctttttctc cagaaccttc tacgatcgtt gtattgtctt tatctacaac aactttagaa
      841 gcatttccta agttttcgat tgttgcatct ttcaattcta aacctagatc gtcagtaatt
      901 actgtaccac ctgtcaaaat agcgatatct tcaagcattg ctttacgacg atcgccgaaa
      961 ccaggagctt ttacagctac tacgttgaat gttccacgga ttttgttcaa tacaagagtt
     1021 ggcaatgctt caccatctac atcgtcagca ataatcaaca atggacgtga ttgttgcaag
     1081 atttgttcta acaatggcag aatatcttgg atgttagaga tttttttgtc tgtgatcaag
     1141 atatatgggt tttctagaac agcttccatt ttgtcgttat ctgttaccat atattgtgat
     1201 aagtaaccac gatcaaactg cataccttct acaacatcta gttctgtttc gatccctttt
     1261 gattcttcga ttgtgatgac accgtcgttt ccaacttttt ccattgcatc agcaatcaaa
     1321 tgtccaactt tatcagaacc cgaagaaaca gctgctactt gtgcaatggc ttcttttgaa
     1381 tcaacaacag ttgagatatt atgcaattct tcgactgctg cttttgttgc caattcgatc
     1441 ccgcgacgaa tgcctagagg attagcacct gcagttacgt tttttaaacc ttcgcgaacg
     1501 attgcttgtg tcagaacagt tgctgtcgtt gtaccgtctc ctgcaatatc atttgttttt
     1561 gatgctactt ctgatacaag ttttgcaccc atgttttcaa aatgatcttc taattcgatt
     1621 tcttttgcga tcgtcacacc atcatttgtg atcaatggag agccgtaaga tttttctagt
     1681 acgacatttc ggcctttagg tcctaatgtt actttcactg tatctgctaa tttatctacc
     1741 ccgcgtaaca tagctgcgcg tgcgtcttct gcaaatttca attcttttgc catgattgat
     1801 tcacctcatt gattattttt gattattttt ttatcttcca aatttactca acaatcgcca
     1861 tgatatcttt tcctgcgacg atcagatatt cttttccttc gtattttact tctgtaccgg
     1921 cgtatttttc gaacataact tggtcgcctg cttttacagc ggcaggaact ttttcaccgt
     1981 tttctaacaa acggccttca ccgactgcaa caactgtccc agtttggggt ttctctttag
     2041 cagcagaagc taaaacgatt cctccgacag ttttttcttc ttcttttgcg acttcgataa
     2101 tgacgcgatc acctaatggt tttaacacga taaatccctc cgataactct ttttttaatc
     2161 tgttagcact cttaaccttc gagtgctaac ctacattttt aataatactc attctccctc
     2221 ttttttgcaa gcttttaaat caaagaaaag aaattttatt ctgacagctg cttattgaaa
     2281 ttatgatata attgaaatca atcgatataa gcttgccttg catgcttgtt atcccttaaa
     2341 aggaggaatt acatgtcttt aaaaaaatat agcttcctca gcattttttg ctatggtcta
     2401 atttttatct cacccttgat cctagcttcg ataggacttg taaaatctac gtctgaattg
     2461 atcacagcaa ctgcagtcat gtatatacta ggcgccgttg tcctagctat tttttatttt
     2521 aagcaacgtg aacccttagc gatcgaatca gcggtaaaaa aagcttctgc tccaaagatt
     2581 gttatttatg gattggttgg aatctttgtc gcattgattc tgcaaagcat cgctgtcatg
     2641 ttagaatctg tattgtttgg caaagcaacc ccttccgaga atacacaaaa tatcatccag
     2701 atgatcatgg aagcacccgc ttttattttg gcgacaactg ttgctggacc catcatggaa
     2761 gagttcgtat tccgccgcag tatactgggg atcatcagtc gatattcaaa tttttgggtc
     2821 ggagcagtga tcagctcttt gctgtttgcg tttgctcata atgatggtca tctgctaatc
     2881 tactttttcc taggtttctt cttcagccta gagtacaaag caactggtcg gatctggaca
     2941 tcgatgatca cacacgtagg aatgaacaca ttagttgttc ttgttcaatt agctgtccaa
     3001 aaagggctag tataaacatg actataaata ctcctgtcat cctagattgg tgattttccc
     3061 aatctaaagc gacaggagta tttttgtttt ttagtcgttc atcttttctg ctgctccatc
     3121 attttttgtc tgtctttcct ttttgcagtt tgcagaatct atttttttca aaaatgggaa
     3181 ataaatgaaa aaactaattg acaacacgat accttgtaaa atcgctgcag gcactccgcc
     3241 tactaagaaa cccgagagga tcggcggtgt tgtccaaggg acttggactg ctgtaaataa
     3301 aggaacgaat ccaaaatata aagcggaata ggtaatcaat cctgaaacga ccggtgcgaa
     3361 aataaaagga acagccatca tcgggttcat cacgatcggt gtggaaaaaa taattgattc
     3421 attgatgttg aaaaaggctg gtaaaagagc aagatttcct aattctctgt atttcttcga
     3481 tttcgcaaaa accgtcataa aaaatacagc ccctaacgtc aatccagcac ccgtaaccgt
     3541 catgaactga tcgagaaatt gctgagtaac gatatgtccg ccatttgcca ccgtcaactc
     3601 ttgtcctgag gctaagattt ctgcatttgc taatgaattc gcttgcaaga cgggactaac
     3661 caacccattg accatcgcag atccatgaag accaaataac cataaaaacg gaacaaaaaa
     3721 acctaataaa agcacaccaa acaacgtgtc tatgaacgat tgaatgggaa gctggacata
     3781 tgtccatact ccttgaacaa cagtcattct gaacaaatgt tgaaaaaccg ctgaaccgat
     3841 aagcgtaaaa gtgactataa caatgattgg aatcaatgaa gtaaaagact gccaaacatt
     3901 ttcaggtatc ttctctcttt gcttccaccc tttcgcaagt cttactaaac gtgcatagcc
     3961 ccatccgatg atgagcccag ttgtaactgc tcctagtatc ccttcagcac ctgtccagac
     4021 agtatctatc tgagtattta gtggctgaat aagcagtaga taagcagaca aggctgttat
     4081 gccgactggt aagcgatcga tgtcttcttc ttttgcataa gcgtatgcaa tggcaaaaac
     4141 gatccaaaag gctatcacat caaatgtagc ctggtatact tgtgtaaaat acatagaaaa
     4201 tcctgttttg tctatccact gagcgatgta atccacagga aaatgagcaa ataagaaaaa
     4261 cagcgagcca acaataatta ttgggatcgt gtaagtcatt ccattacgca aagcagttaa
     4321 gggtctaaga ttcacaacag atgttatcat tggtaaaaag gcacgatcta accactcttt
     4381 catttcttat ctccttcaac ttctattttt atcaatcagt tttcttaatt taactatacc
     4441 caatgatcac gaaccatgaa aggaatgcgc actgagcttt tcaaaaatcg gattctttga
     4501 cgaatattca gcaactgctt acaatggaca ggaataaaaa aggaggtttt ttatgaaaat
     4561 tgcactcatt ggagctactg gccatgcagg ttcatttatt ttagacgaag cattgtccag
     4621 agatttagac gtgacggcaa tcgtgcgtca tccagaaaaa ttgccatcag atgttccgtt
     4681 tatacaaaag gatttatttg agttgacaac tactgatctt gcatcttttg atgttgtcgt
     4741 tgatgctttc aacgctccca aaggaaaaga agaaatgcat caaacttctt tgtcccattt
     4801 gattgagatg cttgaaggaa ccgatacacg cttaattgtc gttgggggtg cctcttcgtt
     4861 attcttagat acagaaaaga cgactcgtat gattgaccaa gtacctaaaa atgcaccctt
     4921 ctatccaaca gctttcaata tgtacgaagg attgttaaaa ttgaaacaat ctaaaaatct
     4981 acattggact tatatcagtc cagcttcttt atttgatcca catggcaaac gtacaggtac
     5041 ttatttgtta agtgatgatt atttacaaag aaacgctgct agcgacagta cgatcagcat
     5101 ggcagattac gcgattgcat tagtagatga gatactggat gagaaacatg aaaagcagca
     5161 catcagtgta gtcagtcgat aatacggtta tttttttaat atgctaaaaa aataaaaata
     5221 gaagataaaa ccaaccctga ttgattttat cttctgcttt ttatattttt attgattgaa
     5281 ctgtttaaat cgaaaatagc tcagttgctt catctggatc tgtatcatag atttcaaaat
     5341 catgtccaac gccaaaatct ttttctttca aaacattttc cattgttaaa tgagcaagtt
     5401 ctttcatatt gttttccttg ctaagatggc caagatagat tcgtttggtc cgattaccga
     5461 tcacatcagt catgacaagt gcaccgtctt cgttggaaag atgtcctcta tctcctaaaa
     5521 tacgctgttt taaattccac ggataaggtc ccatgcgtaa catctcaaga tcatgattac
     5581 tttccatcaa ataaccatcg gcattttcaa tgacacctcg catataatca ctgcaatatc
     5641 cggtgtccgt caacatgacg aatgaccgat tttctttatg gaaacgataa aattgcggag
     5701 ccgctgcgtc atgggagacg ccgaaactct caatatcgat atcgccgaat gttctgactt
     5761 tccccatctc aaatagatgt ttttgttcca ctggtacatt tccaatcagt ggttccatcg
     5821 cttcccaagt ctgctgattg gcaaaaacat ccaaatgata cttacgagcc aagacaccca
     5881 cgccatgtat atgatctcga tgttcatgcg tcacaagtat tccatctaat tggtccggtg
     5941 tccgatcaat ttgagccatc aaggaagtga tttttttacc gctaagtcct gcatcgacca
     6001 aaagccgttt tttttcactt tcgatataaa gtgaattacc cgaacttcca cttgctagga
     6061 tactgatttt aaaagctggt ttttcttcag ccatttgact cctacttccc ttcttacttt
     6121 gtaaactttt ttcattatac tcgctttttt attgatcttc cacttttggt acagtattgt
     6181 ttgtcacgat cgtattgctg aaagcgttga cgctctcaat ttggaccact ttatcgcttg
     6241 tttcgatcgc tacatgccaa acaggaacat acacattttt ttcccggact ttcagaatac
     6301 aggaataagc tagctgacgc cataaaattt ttgatcctct tggtattttg ttattgatgt
     6361 acaatgtatt gattgcttcc tcttccgtat acagttccgt cttttcgcgc agctgctcga
     6421 tatcagataa atgcgtctgc atgtatttcg tgatgctata atcattatcg tttttttcta
     6481 aagaaagaac gatttttgct gtatcatcat tgaatgggat gccttcatac tcttggcttg
     6541 cttggatttc tagtggttct tcttctaaat gagaaaagtt tttcgtgtac acataatcat
     6601 taccgaataa aatattgtct ttgttagata aaaaatcttc caaggaacta ctaattttat
     6661 ctggatcgat cacgttcttt tcagcatctg aactggataa agatttcgtc aaaacatcac
     6721 cgtctaaaaa cacaccactt gccagcaagc cgcttttacc agccttgcga taattcgcta
     6781 atgaagtgct aagattggtc tgctccgcgc taagataata gccttgcttg ttctcagacg
     6841 aaatcttccc cgtgtaagtg atatcgtcgc tagccagacg ttgttggatt gattcagtct
     6901 gattagagcg aatgacattg ttttcttggt ataccgcgtc atgataactg ctgaataaaa
     6961 acacattcaa ccctaaaaaa gcgacgaaaa atatccattc gatttttttg aaatccaatt
     7021 aattcgcctc caatccaggt aatttttcca gaaggtcgtt agctgaatac cagttatctt
     7081 tatatttcac ataccattca ggtaagagat caacgacacg attcgtttct ttgacatttt
     7141 gccatgtata gccaacgatc actgaaccaa tcttgttgct atctgctccc ttagctttta
     7201 actgctggat gatttcattc gtacttgcaa gttctacttc ttcgtctgaa ggtatcggca
     7261 cttgaatcgt attcaaactg gtttggatat tcacttgcac agaaccattc gtttcaccgc
     7321 caacttccac accaatctgt cctctgctgt ctgtcccaaa aaccgggaag ccttccacga
     7381 atatccgata atcgatcgta ttgtcactgc ggtcaaaata tctgaggttc cctaaagaac
     7441 tgcctaattt gctaatatag gggaaacttt cagaaaatac actagcattt tcttttttct
     7501 ctggcagctc tccacgaaaa tcaactactt gcgtttccga atggatagac attgtttcat
     7561 tcccaccgta aaaaatcaat tcattgcttt catcattatt tttcacgcta tctggtttct
     7621 ggaaaaaagc atttctaaaa agtgtatagg gttgctgtga caggatataa ctatacttct
     7681 tcagtttgat cgaatgtttc gtagcatatt gcgtgtcgat cgtcgatggt tctgttgtca
     7741 tttctgtcca ttttgtgtct tttgctgtta attctttatt gatatctgtc caattcaacg
     7801 aaatatttgc ttcatatacg agatggttcg aataattcaa aaatcggatt tttttatttc
     7861 cttgatcgaa ttgtattcta gtaaaataca tattttcgct ttcttttgcc aagttcgaca
     7921 catctaaatc taaatcaaat gcttccttgt attctcttag caaaaaaggc gcattatagg
     7981 ccaactcaaa accagagttc agatttttca cctgttcaaa ctgtgatttg tctcctgaaa
     8041 ctacttcaga aagctttcca aatcttgctt tatcgatgat caattgcaat cggtacacga
     8101 gattttcact gttggtttct tggatcattc ccgaatggtg ccatgtaaca tgaagcggaa
     8161 gaaagacttc ggaagttttt cgattgcttt gagactcgat catttgagtg tttgtctcat
     8221 ctacgttgat tgaacttttt cctgctgggc tcagccagat tgcataagag aagtatatgc
     8281 ttaacgcaac catcaaaatg agtcctatac gaacgacttt ttcagagatt ctcattccca
     8341 ccattcctcc tcataaggtt catatggcag cgagatataa aaagtcgacc cttttccttc
     8401 cttgctctct gcccaaatag cgcctcgatg cgccttaatc acttctctag aaatagctaa
     8461 tccaagtcct gtcccacctt gtgctcttgc acgtgcttta tctactcgat agaaccgctc
     8521 gaacactcgg ttcagatctt ttttaggaat ccccaatcct tgatcggtga tacttaaaat
     8581 cacgttatta tgcgtttcta ataagcgaca agtaatcgtt cccccatctg gtgaatactt
     8641 gatcgcgtta ttcatgatat tatcgactac ttggatgatt ttatctgtat ctacttcgac
     8701 ccaaagatct ctttgcgtaa attcacgacg gatcttataa tttttttcct gatttcccac
     8761 catcatatca aaccggtcaa gaacaaaatt cactaactcg ttgaaattga catattctaa
     8821 ttgaagctgg gtattacctg tatccatgcg agatagattt aagaggtcat tgatcattcg
     8881 aatcattcgg tctgtttcat ctagcgttac cttcaaaaaa tttggtgcta cttcctcgtc
     8941 tttccacgca ccttcgctca atgcttcgat ataactacgc atacttgtca acggtgtacg
     9001 aagttcatgg gaaacattag agacgaattc acgacgttcc cgttctgttt tttcttgttc
     9061 tgtcacatca tgcaagacag ctactaatcc gctgataaaa cctgattcgc gacggatcat
     9121 agaaaagtct actcgcaaga tcatctgatc actttccaat gtactggttg aacggtcaag
     9181 taccatttcg tttggatctt ctaataattt ccgcaacgta tattcttctt cgatatctaa
     9241 aagtgtcaaa atcgattgtc cgattgcttc ctcgtttttg atatctagca aggaaacagc
     9301 catatcattg atcgtaatga ctttacccct gcgatctgtc gcaatgacgc cgtctgtcat
     9361 atgggatagc acgctatcca aacgattgcg ttcagcttcc attgtatctt gagcttcttc
     9421 aatacgttca gctaactgat tgaaggtatc agccagctgt ccaagttcat cttgtccatg
     9481 cactttgact ttccgactgt agtctcctct agcgattcga atggcttgtt ctctcatttc
     9541 accgatcggc tgtgtaatcg aacgagcaac taaaacagat acgatcattg aaataaatgc
     9601 cgcaattagt gaggcagtaa agaaaatgac tgctgtgtta ttgatttccg aatatttttg
     9661 ttcgatatta cttttgacgt aaagagcccc gataacggca tcgccggtcg gggacaggat
     9721 cggttgaaca ttgatattca cccgtttatc gtcgttatct aaagcaacat aacgtttagt
     9781 cgtgaaatca ttgatatcaa cataatcatt tttctttccc acagcgcttt gttcattgac
     9841 atcggttgtt cctcggatga tccctttttc gtcaacgacc cgtatttcga tgatatcgct
     9901 agtcgcactg ttttcaataa atcttttgat attcgcattt acctcttcat catctgcatt
     9961 atcttttcga ttcaattccg ggctgattgt tgttgctaat gattccaccg tttgattcat
    10021 atctttaata aatgtattga tcgtggaacg ttctaaacca cgtataaagt aggcaccaat
    10081 aatttcgatc gagatcaata aaatcagaat aaaggtaata gcgatcttaa aattgactga
    10141 tcgaaaaaag cggacttttc ttttcatcca attactcctg ttctgggtta cgcaaatagt
    10201 aaccgacacc acgacgtgtc actaaataat ttggatggct tggattatct tcgatctttt
    10261 cacgcaaacg acgtacagtt acatccactg ttcgtacatc tccaaaataa tcataacccc
    10321 aaaccgtttg taataaatgt tctcttgtca tcacttgtcc aaggtgcttc gccaagtaaa
    10381 agagtagttc gaattcacga tgtgttaact cgattgtttc tcctcgtttc gtcaccatgt
    10441 aagcttctgg atggatcgtc aaatcaccaa tcgtcaattc cgcaggtgct gcctcttctg
    10501 gttccttcgc agccgtggca ccgcgacgaa gatttgcttt gacacgtgca actaattctc
    10561 ggttggaaaa tggcttggtc acgtaatcat cggcgcctaa ctctaagcct aggactttat
    10621 caatttcaga atctttggct gttaccataa tgatcggcat atcataagtt ttgcgtacct
    10681 cacgagcaac ttctaagccg tccattttag gtagcatcaa atcaagtaaa atcaaatcag
    10741 gttctacctc agcaactttt tccaaggctt cttccccatc atatgcagta taaacgtcat
    10801 agccttcttt ggctaaattg aatttgacga tatccgaaat cggcttctca tcatcgacaa
    10861 ctaaaacttt tttcataaag agccacctct tatatattat tgtcatacct tctcattata
    10921 ccgtatttct gcttttgttt catctttgcc tataaaacaa aagagaaaat taaaatgaaa
    10981 gtgcaaaata ctttattatt ttttgttcta acttcaataa aaaaggaaaa cactgatttt
    11041 cacctcagtg tttccttttt ttgatttatc cttctaaaac ttgtacaccg acacccaata
    11101 atcccggacc agtgtggact actaatgccg gagaaatctg gccaaagtag atttggtttg
    11161 cttgtgggaa ctctctttta agtctcgctt ccatttcaat tgcttcttct ttcgcatccc
    11221 catgggcaac agctaagttg aagacctttg cattaccgat tttttctttg acgtaagcaa
    11281 ccgttttatc caaacttttt ttacgtcccc tagcttttcc tactgtgtaa taaatccctt
    11341 cttcattaca tgagatgatt gggttaagct ttaaagcggt tcctacaata gaagcaacta
    11401 aaccgatccg tccacctttt tgaagatatt ccaatgtcgc aacattaaag aaaacttttg
    11461 ttctttcgac taactcattc agattactga tcagttgatc ccattctact ccttcttcca
    11521 gccattttgc tgcttggatc gcagaaaaac ctgcaccaat accaatattt ttcgtatcta
    11581 gtacatatgc ttccaattct tcttgctgtt gcgcaattaa acgaaccaca ttatatgtcc
    11641 cgctcaatcc actggaaatt gttaccgcaa gtaccttttc ataaccgtct tttttgattt
    11701 gctcaaagat ttctgtgatc gactcaccat ccggcaaaga tgtaccaggg atttcctctg
    11761 gcaaacgttg atagatctct tcaggggtaa tgtcaatttt atctgtatat acctgatctt
    11821 tataaataat tttcaatggg atcatataca tctcgtattt ttttatcaat tcttctggaa
    11881 catccgtacc tgaatctact aaaattgcga ttttattgtt tttcatttgt tttctcctct
    11941 tttaaatatt cggtttctag aacaatcgtt ttttccgcaa gaagtttcat tgcaaacgat
    12001 aaagttgccg ctcttacagc aatatattct acaggggttg cctggtcgaa tttttgcgga
    12061 tgagatttac cttgtgctaa ttggctgacc atccacacag ctgcttcttg ttcatcgcaa
    12121 aatagattat aggcttcacg aattcctact gtttttcctt ggaacaatat cccttctttg
    12181 atttcaggaa tagtcaatac ttgtttcaac gtagtgattg caagtaaaaa tgctacgtgt
    12241 tctttgttat atcttttttt aactggtgct ggtatcaaac ctagtttaac gtaattgttt
    12301 accatagaag aggtaagcaa tggatgcttt tctccttgta tcacaggtga aagataacga
    12361 tcaactaacg ttctgacttg gtccatataa agttctaagt cagggagttc ttcccagtga
    12421 ggcagacgga atgtttctag ttccttcccc cactccatta attgctgacg cattttttcc
    12481 atcataaccg ctcctttttt catttcataa acatgatatc agacttatga taaacattca
    12541 atctagttgc tttaactagg tgatttgatt agcgggattc catgaaaaca aaaaacaaga
    12601 aggctaagcc ttcttgtctt ttatcgttaa gaacaggaga cccgccgggg agacggcggg
    12661 aaaggagtaa aaaatgaaaa aagtgttagg gtttgttctc ttgcaagaac tttgtttgtt
    12721 ggtatgctta tataatagcg agcaaatgtg aataatctat gaatgatttc ctataaggaa
    12781 gtaaagattt ttctgatttt ctttaaatgt agtcttgcat tttaaaattc ttagtgctac
    12841 tattattaag tactttttgt acgggccgtt agctcagttg gtagagcagc tgactcttaa
    12901 tcagcgggtc gcgggttcga gcccctcacg gcccattggg tgccaaaccc acggaacagg
    12961 taaatgttgg tgcgaaagca tttcgaaaac atttacgctg ttcttttttt atttttcaaa
    13021 ataactaggg ctttttattt ttaactcaaa gtccactgta gccctttgga ctttgagtta
    13081 aaaaagaagc ataattatga acagtaaagt ttctaatata acaataattc gagaagcttt
    13141 gcaaaatcat gctgccaatg aactctctaa acatatagga ttaaatctaa gtactataaa
    13201 aggttaaaaa gtgaagaata tttaatagaa aaactaaatt tacttgctgc tttccccttt
    13261 tccattaata caacaatcag agagtgtctt tttttcgaaa aaattagaat aaataaaaga
    13321 cacgtagttt ttttatctac atgtcttcca tcgactacaa gttatcattt ctcaaaacgt
    13381 atttagcttt taattgatta atcgtttcac gttgtctaca cattgcctaa ttaagatttt
    13441 cttattagaa gcatcaatcc cgcaataata cctaaaactc caggaattac ccacaataat
    13501 gttaaaagaa aaaatggaat aatagataaa atgagtaaac taataccata gaccactgtt
    13561 ttttgatttt ttcttactaa aaaacctatt atgaaaatca ttatcgaaaa aactagggta
    13621 attcctccaa ataaaacaac attcccgctt agattgacta acatatctat atccttactg
    13681 gttagttctg tagtatttaa agtagttgaa tcaatagaag atagaaacat accacccatc
    13741 aatgctaaaa gactacataa aatccctgtt attccactaa ttaaaatcaa aataaactct
    13801 gtttttctct tattcattta attctctcca gattaagtgt tttagaatta gaaatttttt
    13861 gaccatagtt aatagcttct aatgaacgaa taacaaccac tgacgtgtta gaaaaataat
    13921 ccccaatatg ttgtttcttg ggcgtgaaag cagccaccaa atacccaatt tgaaatacaa
    13981 aattttttaa tataaaccta catatacact cacgaataaa gatagtttgc caacttagct
    14041 ctttttcctc aaagctcact actcttatcc caaaaatcat tttcccaatt gtttgtcctg
    14101 aattcagctt agtcaaaagt acaaaatagg ttaaataaat agctaatgac gctataccat
    14161 aaaaagtaat aatggatttg tcagattcca gtcccatgat tttgtaaaag agacctatag
    14221 tagcatgggt gatgatgcta atacaaatta aatcaataag aaaagcaaaa aatcgtaacc
    14281 aaaatcccgc aaatacataa ttaggcaaag tttcaatgcc actcttctta ttcattttat
    14341 agccctccat agtaatacat tggttttgga gtatcagaaa atccaagttt ttcaaaaact
    14401 gaaattacac gatctgatgt agttgccttt agcccttgcc attcagcaat cttattacct
    14461 aaccaagaag aagcaaagcc agttgtgtca tttttatatt ctataaccgt cgcatcagtt
    14521 aatttcttct ctttttttag ggaatctaat gcatcttcag agtaaccaat ttgatccacc
    14581 aacccatttt ctactgcttg gttaccatca taaattcttc catccgcaag ttctttcact
    14641 gcattagtat ccatatttct tccttttgct acaacattga caaaacgatc ataagcactc
    14701 atcacaaatt cctgtataac tttattctct tcctctgaag gtttgtgttg aggtgacatc
    14761 atatctttta atgcaccact tttatacgtc gcatcagtta cgccaagttt ttctaataag
    14821 ccagaataat ttaaaccaga aataatgaca ccaattgatc cagttgttgt ttcttccgta
    14881 gcaaatattt tatctgaacc agcagaaatg tagtaccctc cacttgccgc tgtattttta
    14941 aatgcagtat agataggaat atctagcttt ttaatttttg ccatttcttt agcaatctca
    15001 gcactttcat atattcctcc cccaggtgaa tttacttcta ataacactcc cttaaccgct
    15061 ttgtcttctt gaattttctt tagctgtgtt aaaaaattct gatggttata ttgttcccta
    15121 ctaaaaagat tgctttcacc tgtatctgca ataactccat tgacggatag tttgacaatc
    15181 tttttatttg atgcgccttc ttctaacgtt tcttctgtga tttcgttaga tccgtaaaaa
    15241 atcttattta ttcctgttag ggttgtattt gatgcttcct ctttatcttt tcctggaatt
    15301 agcgcgacga taatggatag taacaataat ccgcacgcta ctcccacagc aatccatctt
    15361 cttttattca tataaactcc tcctatattc ttagtaaaaa taattatttt ttatttagaa
    15421 acacatcgac aatttgttgc caatatgttt tttctcctac aattaaagac aactctccag
    15481 ttaatccata acgaaacgga aacgattgag ttgtttttaa cgttcctttc actgcataaa
    15541 aaacaccttt ctcagtggca gtagaatttt ctgaaatttc atctaaagtt ccgtcgatcg
    15601 taatcggagc cccacctttt tgatccaatt taaaatgaac cttcatccct tctttaatat
    15661 aggtactttc attcgcaggt aaaagtgctg taaatttcat ttttgatttt tcgtggatcg
    15721 gataaatttc tgcaagcact gttcctttgg ctagttctgc ttgtcccttt gtttcttcat
    15781 ttaaatgaac cgttccagat acaggagctt ttagtattcc ttttgaaagt tgatcgtcca
    15841 aacttttgat aactgactgg ttcttctctt gtttttcatt tagctctgta actgtttgtt
    15901 ttacagttgt tattgcttgt tctagctctt gctttccttt ttccgtttga ctaattctgt
    15961 cattctcaga agtagttggt ttaactaatt tagctttttc tgactgatac tgttctactt
    16021 ctttttttag ttgctcaatt tgttcattta tgcttgctga aattgtcagt ttcacttgat
    16081 ttttttgatc atcagaaaca ttatttagtt gttcttgcca attctcatag tttgccatca
    16141 tttcttttga aaaatctttt aatgattgat tattactcca agctacctgt acttgttgcc
    16201 attcttgtaa tttactatct gaactctcaa tttgtttttc tagttgtctt tttgtctttt
    16261 cgtatacttc taattgcttt tgatcattta attcactttg cttcaaagcg tagcggagtg
    16321 attctttttc tgacaacatg ctctttaatt gattactgta gccaaaactg tcatccgttg
    16381 aaaacaaatt ttttccttta cttatacttt caataaatag ctgagccatc tttatttgtt
    16441 gagtaatacg ttggttttct tgttctatct gagccttttc attctctatt aaagaacaat
    16501 ccaatctaac caacacatcg tccttatgaa caaattgatt ttcttttagg aagttttcca
    16561 ttattttagc ttctaatggc acttgtaact tctctatttt ttcggggttc aattgagccg
    16621 ttgtccgaat aacaacctct tttttagcaa aggttaaaaa caatccaata atacataaaa
    16681 aaagaattac agggtataac acccaacggt agaagctatg atgttgttta gtatagatac
    16741 tagaatgttc gaaccaatta ttattctgca taaattgttt ccctctccta gatttcccat
    16801 aatttttgat agataccatc ggcaaaacga agttcatcgt gcgttccttt ttctaccaaa
    16861 tgcccttcat ggagaactaa aatttgatca catgcttttg ctattggtaa atgatgcgca
    16921 ataaaaataa tcgttttttc ttttaaattg aggagataat tcataattgc atgttctaac
    16981 aaggtatcta atccactagt tgcttcatct aaaattaata tgtccggatt ttttaataaa
    17041 gcccgtgcta aggctaatcg ttgtcgttgc cctcctgaaa gattatttcc accttcttcc
    17101 aacaatgtct cgtaacgtaa tggctgttga ttaataaatt catttagttg gactatctca
    17161 caaacttcta ttatttgttc gaaacttggt gggctatcta aaccgaatat aagattctca
    17221 atgattgttc cactaaagaa aaatgactct tgaggcaaat atgtcactcg atcgcgtaat
    17281 atctgaaaag gaatgtctaa gtgattaatg tttccataat ggatcgatcc ttctgatgct
    17341 tcataaaaac gaacaaataa ctttgcaaga gttgattttc cagaaccact aactccaact
    17401 atagccgttt tactgtgcga aggaattgta catgaaattt cgttcaacgt tggggccttc
    17461 atgctatacg agaatgaaac gttttttaca gtgatatctt gttgaaataa ttgttcagaa
    17521 agcatatatt caaaagactg gttcgatttt tctgaatcaa tcgcgaaaat ttcatttaaa
    17581 cgattatttg caacttgagc cgtttgcatt ttcacttgta aattaataat gttttgtaaa
    17641 ggatctgtca aaaatactag taaagcattg tatgtgataa gttgacctag actaattcta
    17701 ttatccataa caaaataaga acctagccat aaaattaatg cactgctaat taattgaatc
    17761 aaatgtttaa ttccttgttg aatattatca agcgtaaccg tacgaaatga ttttttcatt
    17821 aatttaacaa attcatggtc tatacggtta tatactttct cttcaccgct atatgcttta
    17881 attgtttcta ttcctttaag actttctatg atactagaat ttacagctgc accggctgcc
    17941 atttcttccc tgttcgattt ttcatagctt ttcacgaaag cgaatattgc aactacataa
    18001 aaaggcaaag agcatagagt gattaaaaac aacgtactat tctgaatagc taacgtaacc
    18061 ccaacaagca acaccatacc aatatctaaa aatattgata acgtagcact tgctaaagca
    18121 tcaataatct tactggcatc aagaaaacga gaaataattt cccctgattt tctcgtagca
    18181 aaaaaattca ttggtaaagt taacacatgt ttgaaatact gaagcataat tgccatactc
    18241 attcgctgtc ctaaaataat aagaagataa ctacgactat attcaaacaa tacccgaaat
    18301 aaatagacag cgatcaagcc aatggaaatt atatttaaag ttgaacgtgc ttgattaggg
    18361 atgaaaaaat caagaatacc ttgaaaataa tatgagcttc ctatgccaaa aaacgtaatg
    18421 aaaatcgatg ctaaaacgat gtggaatact aatccttttt gtttccaaat tataggcaaa
    18481 aaagaactta atccagctac tttttcttta ataggaatat aagtttctct aggcataggt
    18541 aataaaagaa tacctgtcca ttctttttga aacgcttcaa tcgtttttct attttttcca
    18601 ttagcgggat ctgcaatcca tagtacgttt cccttcgttt tataaattac aacgtagtgc
    18661 atatagctgt tatcaaccaa tatatgtgca attaatggta agataagatc tctttctttc
    18721 cagatctcac tatctgcttg aatagccata caatcaaaag acaatttttc aaatgtcttt
    18781 ttcaatccaa atgctgaagt accctctaaa tcagtcccag ataggtcccg taatttatga
    18841 atcgggatat cagttttaaa atattttaaa atcatcgaaa ggcaagcaac accacaatct
    18901 ttttcatctt gttgctgcac aaaaggaaat gattttatca cttatctaac acaactcctt
    18961 ctaaaaaatt tttatagaaa aattctcaat atagctttaa tacagtgaaa atattgtctc
    19021 ataattccac aaaaactttg aaaacagttc ttgaacacta actttttcat tcctgaatca
    19081 aatttatttt ggaaatttta ctattcaaga ataaacttga taagtttcag gaagattttt
    19141 actgttaatc aaatacgtat gttacagtag caccgtaaaa aatattattt aaagaagcgt
    19201 gtttaaacat gcaaaatgta aaggaactaa gtattgtgga aatgcacaaa ctgttggtgg
    19261 aggcaataat gcttggggaa aattggggca agttgtaggt ggacttacca caggggcagt
    19321 aggtggtgca ggtttaggaa cagcaatttg cggaccagct tgcggtgttg ttggagggtt
    19381 atatggagct gtcgctggag gtgctgcagc tggctgggat gctcgaaaaa aataaaaaag
    19441 gagaataagt agtatgcata ttaaaaacac aaaaacaaca tttatattat cttcagaaga
    19501 gttgaaaaat attcaaggtg ggagtgccgt gggagtttta ggcactactt tttctggggc
    19561 aacagcagga gtaaaacttt gttcagccgg aggaccttat gcaatagctg cttgtggtgt
    19621 aggtggagct ttattaggtg ctggattcag catgtggacc ggtgcgtaaa caattaaaaa
    19681 atatcaaacc tcaagattgg tttgtgtcga ttacgaacat aggtatgttc tttatctttt
    19741 caaaagtttg gaatggacag aactttttac taaagcttct tgtgctagtt tttataatta
    19801 tattaaattt ttatctttta ttaaagtcag ataaaaattc tttggaaatg taatggtaga
    19861 gatattttta caaaaggact tgcagcgctg tagttggtgc tgcaggagga cctatttcgg
    19921 ccgttacagg aggtattggt aatgtacttg tcggatgtgc attaaagcct gttcctgcaa
    19981 gttaaattta taaaaacagg aaagtaatta tctcaaatcc tatcaagtac aatcatagga
    20041 ttgcacttga caggacaaat tttttaacta aaaaatgaaa aacactttct aaaaattagt
    20101 agaatttgcc aaatctttcg catcttccac catacattta attttaattc ccctttatgg
    20161 tacttaataa aaacaagaaa agaactattc atttggccct tttttggttc cagaccatag
    20221 aaatattcct ccagtaacta atattgctgt tatgcacgta actaataaca gctataaaaa
    20281 ttggtacaac tttaatagga ttcagaaaaa tttgcactag tgaaggaatc tattttatta
    20341 gaaaaatgca atctccctaa agaaaaaacc actaaacagt gctttctttc aacttctcct
    20401 taattttttg tgctttccct aaagtaaagt ttctggccaa ctctgaatca aaatatagtg
    20461 aacgtttttt aaaatctatt tctttaacgt taaagatatt tactaaacac gctcgactaa
    20521 ttcttgttaa cataggatat ttcttttcaa gctcactaat tcttccataa aattcgtact
    20581 gcccatcttt agtacacaat gataatctat gtggtaattt tgaagcttct acaaaataaa
    20641 tgtcatttat atcaaaagta aatgtttgag aacctatgga aaaaacaaat gcttgattcg
    20701 taacctgttt tgttgcatct atcctttctt gcgctaataa caataactca acaatctcgt
    20761 ttcgatactc atctagtgtt tgatcttttg ttacaaatcc taacgtttca actcttcttt
    20821 ttattgttac gggtaacatc tcatcatgag tagttgtaaa aatgattttt gcttgaacat
    20881 catattttct gattacttct gctaattcta taccgttaac ttcatgattt aaatcaatat
    20941 ctaagaaata tattccttgc tttggtcgga attgtttgag gtatttttta acttctaacg
    21001 gactttgtgt tttcaatact atcttaaata ctttatcgtg aaaaagaatg aaattatcaa
    21061 taattcgttc taactgattt aattgtataa tttgatcttc acataatatt attggataac
    21121 tcataaatcc tccttccctc aaaacaacaa aataacttga gtagtaaatt gatacggatc
    21181 gttttgatag ttcacaaaca tattgggatt ctttttattg atttcctcta tactagataa
    21241 acctaaccct tgatgtccct tttttgttgt tattcctttt ttcattagag tatctataga
    21301 gagtagtatc tctctacaac tattcgatat cgaaatttct atctgtttat catctcggta
    21361 aatagctaga gacagttctc tatcttgact ctctatagat gcttcaattg cattatcgag
    21421 aataattcca agcaccctaa tacagtcaaa tattggaaca ggaacttcaa taatagtact
    21481 tggacattcg aatttacaac aaatcttatt gttatttgct tggtatagct tcgctatcaa
    21541 caaacttttc aaatattcat tttttatatt ttttaaataa cgatattcaa acttattggt
    21601 tagataagaa ttagaataga tctctaattc ttttatttct ttttccaata tcgaatcatg
    21661 gttttgatta gcgatttctt ttaaactcag caataaattt ttatagtcat gtctaaatct
    21721 ggcaaatttt tcttgatctt tttccagtga atcagtatat ttttttagat aaaccaattc
    21781 ttttcttttc aattgctgtt catattgttc tcgttgccta atagttattc taataaataa
    21841 aaatagaaca acaaacattt gaatgattaa aaatattaag acaccaaaca caaaatgatc
    21901 aaatacttga tattgatgag caacataaga aacaaataaa ccaattgtaa gtaaataaat
    21961 catgaatata gcagtaagtt tagaactgga ctgttcaaca atcctattta tagctaattt
    22021 tctatataac caaataaaca tcagcaaaaa caaagaatca agcattatct ggaatactac
    22081 ataaccatac ccttttatcc cttcaaatga aaacatatac gtcataacca tggaagtaat
    22141 aatagatctg aaatcatcaa taagcataca taatagtaat ttatttaaaa gcactatatt
    22201 agttttcttc ttttccttta atataaaata acttccgatt ataggtatag ctgcggaaac
    22261 atcagtaaat aattcaacaa aaaaagtgag tggtatcaat aaacatacaa aaagatattt
    22321 tcttttgtta agatcagtat tagtcatata aaataagaga aaaaagaaaa agtttcccac
    22381 gaacattctt aatagataag cttttgtatc gatagtaatc aagagatccc ctcctgattt
    22441 taggatatca taaaaaaaat tttagttaca ctttttaaat aatgaaaaaa cacattccac
    22501 taaatttgat gccggttttc cttgaggttt tgttccagca cctcctttaa tttttttaag
    22561 ctcttgatct gaacattgtt tagcatttag tctatttttt tcttccatta cgattccccc
    22621 tacaaactat aaatactttt atgtcactga ttcaatatac ctatttttct aatcaaaaaa
    22681 ttttgagaca tatttaatat tataagtatc attaaaattg agatttatct ccataatctg
    22741 ctcgatataa accatttatt ccagcagttc ttccaatttc actcaaattt cttattaatt
    22801 cctcttgata ttcagtgaat cttattttat tagtataagc agtaaaatat agataattca
    22861 ccaatctatt tattaaagga ccgggatcta attcatattt ttgtttttct aattttaaat
    22921 ataccttttg taaaacctca agaatatcag aatatttagg atctttactt atagatatat
    22981 cattatataa ttcatgaaca atttgcttag catttttttt cattttagca cttccctgga
    23041 attgctccac ctaaaaaacc acctatagac attcctgcaa tacaagtagt tgccttggcc
    23101 caatcgaccg tacatttatt tttagtgcaa tacactccat ttccataata ttttccacta
    23161 tgagtggtac ccccatagat aagttgtgtc tctttaatag acaaaatttt taaatgtttc
    23221 ataatctaat cacccctaaa ttaatgtttt tagactatta ttctaacatt cattaaattt
    23281 caaaaaacaa ttccttcttg aaacgtagca aaataattcc tgaataagaa tttaatcact
    23341 tttaagtata taaactatgt atattcttta atattttttg agaatgcact tcgtacataa
    23401 taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact cgcacccata
    23461 aattcataat agtttgtcgg cgtctttgga cgtttcgaag atgagctacg cattctcttt
    23521 ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat atttgatcca
    23581 attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct ttcgactaat
    23641 tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc attgttgatt
    23701 tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa tactttgtaa
    23761 gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg ctcttcgctt
    23821 cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa catccaatga
    23881 ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc tagtgcaacg
    23941 gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc atatttttgg
    24001 atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat gattttctta
    24061 cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg cactgctaaa
    24121 ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt atattgtgca
    24181 tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga ctcgccattt
    24241 gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc atagcccatt
    24301 tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca ttcaacataa
    24361 gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc aatcgcacct
    24421 aagatcacta ctactacagc accaatgatc agtccttgac gttttttact tttctgtgtt
    24481 gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa acggtcaatg
    24541 atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac ttgtgctctt
    24601 tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga ggcaataacg
    24661 accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt acttttagct
    24721 aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga atcagaagct
    24781 tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac ggttggtggc
    24841 agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa aaatgcaact
    24901 gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat cggtttgata
    24961 atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc aatgatgatc
    25021 gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt ccatagacct
    25081 tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg acgtttctta
    25141 gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg aatcactaac
    25201 agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa tgaaaatagg
    25261 ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc cactggtaat
    25321 tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta atgcttcaat
    25381 cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg caagtggagt
    25441 cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc caatcaccgt
    25501 cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt ctaacgcttg
    25561 atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca gcatgctgac
    25621 ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga tattcgtcgt
    25681 caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga cttcttccac
    25741 aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg ttccgatctg
    25801 catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag cttcattcaa
    25861 atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca gtaattcgtc
    25921 ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca tcagcaagat
    25981 ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc caccagataa
    26041 ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg ctttttctgc
    26101 tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt ctgtattttc
    26161 caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga ttttatgacg
    26221 acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat caatatagat
    26281 actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg atttcccact
    26341 cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat tcacgtcata
    26401 cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt gatcattcat
    26461 catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt ttacttctac
    26521 ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga aaaaagtgac
    26581 cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt gcttgtccaa
    26641 actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt aatatttttt
    26701 taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt ttgtcatacg
    26761 ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa atttagcata
    26821 ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg tggcgccagt
    26881 cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt tttctgataa
    26941 agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt gattgaccag
    27001 ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca cacttgccat
    27061 gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata ggaaatcttt
    27121 tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca tgattccaag
    27181 atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt acgtactggc
    27241 aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa cgatacgttc
    27301 ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct ttccagacat
    27361 tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc aaaataagca
    27421 aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt ccttttttac
    27481 tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag tatagacaat
    27541 tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc tattcctctc
    27601 cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg aacaaaacat
    27661 aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc tgtccaccct
    27721 ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa ccgcattgat
    27781 agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata gccccaattg
    27841 ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct gctgcgggag
    27901 tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact tccgcatgac
    27961 caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct gtaatcgctg
    28021 cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc aggattcgcc
    28081 cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa ttataagaag
    28141 agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt ttaacaaaag
    28201 acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa tgatatgcgg
    28261 gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt ttcaagctga
    28321 ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct acccaagatt
    28381 gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca gaacaaggca
    28441 tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc gtgcgactgg
    28501 ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag gattttcctg
    28561 aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc atatatccgc
    28621 agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga aatcctaaca
    28681 ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat cccgctactt
    28741 gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac aatgtgtcat
    28801 tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag atcagccaca
    28861 tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga tcgattttgt
    28921 cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat agttgggtaa
    28981 taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat agtttctctg
    29041 ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg taatcctctc
    29101 ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg ccgctttgtt
    29161 ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca tatgtacagg
    29221 gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt agtttttcga
    29281 ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca taggaaagct
    29341 tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc agaccaatga
    29401 tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt tctaaattgg
    29461 tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa tctcttgcaa
    29521 tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc tgaatatgaa
    29581 tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc ggccaattcc
    29641 ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg ctgtttggat
    29701 ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta gttcggcctc
    29761 tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat ctcccagagg
    29821 ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca ttaaccgtcg
    29881 cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg cttttaattg
    29941 atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga tttgacctag
    30001 tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat tgttgcctga
    30061 taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca aatggcgttt
    30121 tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct ttgcctgcgc
    30181 taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa tcattcatta
    30241 tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca catagaatac
    30301 tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct ttagtattga
    30361 tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt ctctattaaa
    30421 tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca caaaacttag
    30481 tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt ttactaagag
    30541 ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca ccatgaacgt
    30601 aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt ttttaacaga
    30661 aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag tagactggtt
    30721 gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa cagttatcgg
    30781 attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga atcaaaacgt
    30841 gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact tccaaatcct
    30901 tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga
    30961 aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt caagctgaat
    31021 aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa agaagcttta
    31081 gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttctatc tattatggaa
    31141 aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca attgtattca
    31201 ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc tttcaaatct
    31261 tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga cggttccgct
    31321 tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc gaacggggat
    31381 gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca gccagctaca
    31441 ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg tttcttgact
    31501 caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt gcaattgtcc
    31561 cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg cgctcctatc
    31621 aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt cgaagatagt
    31681 ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta tttgaacgtt
    31741 ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt
    31801 cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga attgacacgt
    31861 aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata tggtgtccca
    31921 ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc aaacattcgc
    31981 aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca acaagaatct
    32041 ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat cgatggcaag
    32101 atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc tgtgattaac
    32161 ggcaaacagg aatatgaagt attaggaacg gatatctcat gtaacttagg ctcgacaaat
    32221 atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca
    32281 ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca aaacggaaat
    32341 aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct tgcaaaagaa
    32401 caaatggaat atggctcaga ggattcatta gactttacaa atatttactt catgctgttg
    32461 aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca agttttccat
    32521 aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt aactggagaa
    32581 ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat cccaactgct
    32641 gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca tcaaaatcgc
    32701 ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc aagtattcat
    32761 cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat ttattaccca
    32821 gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga tatggacatg
    32881 cgtaaagtaa tcgatgttta tgcaatggca caacaacacg tcgatcaagg catgagtttg
    32941 actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac aacaacgaag
    33001 caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg aatcaaatcc
    33061 atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa
    33121 agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt aaaggagcat
    33181 tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag atatcattga
    33241 taaatccact tgggagaagc tgacagagca attttggcta gatacacgta tcccactttc
    33301 aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag taggacatgt
    33361 atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg atcagttgcg
    33421 taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat tcatggaatc
    33481 cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa aagaaatcga
    33541 agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa
    33601 tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct ttttagaaac
    33661 attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca acaaattggc
    33721 taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg gaacctacat
    33781 cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag aaacgttaaa
    33841 agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt atacagaaga
    33901 attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct ataatgccaa
    33961 caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca atgatgtcaa
    34021 cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct tctcacaagt
    34081 cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt atttgattgg
    34141 attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag agtttagcgt
    34201 acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt ttcttccagc
    34261 acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa atagattttc
    34321 cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat cagcatgcct
    34381 aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa aggatggata
    34441 ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg ttggatcact
    34501 aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc gacaatgatt
    34561 gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat tccgcaaatg
    34621 accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa gctcattaca
    34681 ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt ttcgtttaat
    34741 tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg agacaacgca
    34801 tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac ggtaaataac
    34861 aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga taaactgact
    34921 aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg ttcttccaaa
    34981 ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct tgtttcatct
    35041 gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg aggcactaca
    35101 ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac
    35161 actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat ataatagata
    35221 aaaccagcaa caagcaatgg taaaaaaact gccgaaagca ttttcaatat tggactaaaa
    35281 atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag ctccactgtc
    35341 cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca aggtcctttg
    35401 atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt tgcttaaaaa
    35461 aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa aacacggttt
    35521 ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg ttcaaaagct
    35581 tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt tatttggctg
    35641 ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat tgcgcaatac
    35701 tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc acatcggcat
    35761 aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg tagtcatcaa
    35821 cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca aaataatcgg
    35881 taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt ttagtcgtag
    35941 gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata aaactgatga
    36001 tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt gcccataaag
    36061 aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta
    36121 agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc
    36181 cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc tcaacttgga
    36241 tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata cctgctggac
    36301 gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg atccctaaac
    36361 gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc tccagtccaa
    36421 tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag
    36481 gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc aatcagctga
    36541 ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt tgtcttctct
    36601 aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga aaattacaac
    36661 ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg caatacactt
    36721 gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc agctttgctt
    36781 tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat tcccattgaa
    36841 acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc attgattgtt
    36901 gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa
    36961 ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg tgcatcgatg
    37021 atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc tggtacaaac
    37081 gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg gtagctttgt
    37141 tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat ttcactgaaa
    37201 tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc tgttgccaat
    37261 cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc tttttttccg
    37321 ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt atattttggt
    37381 atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga ccaaattgca
    37441 tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa gatggttcaa
    37501 tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg ctttatgtct
    37561 gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca acattaccca
    37621 gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg ctggcgaaca
    37681 tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa tcatcactgg
    37741 tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca aaggttacct
    37801 cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa aaactgcgta
    37861 cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat atagcgagac
    37921 cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag accaaggagt
    37981 gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga tcgatgtctt
    38041 aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg tgattttagc
    38101 aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca atcacttaga
    38161 tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt
    38221 gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag acatcgagtt
    38281 tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa
    38341 tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc
    38401 ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca
    38461 attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg tctttgattt
    38521 tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag tcatcggata
    38581 tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa atattgccat
    38641 tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg gtgagattga
    38701 aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact tttcacaaga
    38761 tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc aatttccaaa
    38821 agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta atcaattagc
    38881 aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt
    38941 gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata tcgatcaaga
    39001 aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa tcatcgtctc
    39061 tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg aaccaaaata
    39121 aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc acaactcctt
    39181 cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa aacggcaaaa
    39241 tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag cttttcacaa
    39301 cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac aactgtttga
    39361 aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa ccagccatac
    39421 tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa gtacataaac
    39481 ataaatacag tattatttcg gttgcttgct gcttcaccta gcccctgtac tcgtgtttga
    39541 ttggcgactt gaccaaattg attcccgacg tctaataaaa taatagctaa aagcaaaatg
    39601 gccaaatggc ttccgccaac caataaagca ataaagctta ttgtttgcat aacaagtccc
    39661 atatacacga tttttctttc agaataagaa tctgccagtc gtcccacgat aggtgcagca
    39721 actgctccag acaaaccaaa aatcgctaat atccctgctt cgaatgttcc ccaattgtag
    39781 gcgggactgc tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt tccaaacatg
    39841 aaaaatccac tgactgccgc ttctcttaat agacgttgag aagcaactaa atgcggcaaa
    39901 cttttaagtg aatcaaggta tgaaacagct gtactacttt gctggacaga acgcggcatt
    39961 ttccagtaaa gcagaagtgt aagaattccg actgcacata aagcaaataa atagatcatc
    40021 cgccaagaaa aaagactggc aacaatacca gataccgtcc gagaaagcaa aatccccacg
    40081 agtaaaccgc taagcaatgt acccatgacc ttccctcttg cttttggtcc cgctagtacc
    40141 gcaccgtaag gaatgatgat ttgcggcacg atcgaaagta atcctattaa aaaagaagcg
    40201 catgcaaata acgtaaacga aggcgcaaaa aaagcggcca acagagagac ggcagataac
    40261 gctgccattc gaatgatcaa ttttggacga tccaccacgt cccctaaagg aactaaaaac
    40321 agtaacccta acgcatagcc taattgggtc aacatcgtca atatcccgac tgcacttgtg
    40381 ctaacagata aactagctgc tacttttgtt ccaatcggct ggatgtaata catattggca
    40441 acaacaacgc cacaagtgat tgataatagt aaaataagcg aagttgttaa aggttttttt
    40501 tcattactct tcattgattt cccccatttc ctatcttagt ttacaactta tggaaaagat
    40561 tggaaataga aaaatacaac tattcgccac acttattatt tgtgcttttg ttttccttgt
    40621 tttttcttgc gatctttata aatgaagaca actaacccta ctagcacagt aacacttgca
    40681 accaacgtga ctgcagtttt gttatatccg tgaaacagag cttctccacc acacgcatac
    40741 aacacagacg aagggatgac tccaatcacg atcgacaata tgatttgctg tagaggcaat
    40801 ttcatcgcat ctgcagcaaa attgaccata gaagatggaa taaccggtat catatagcct
    40861 aacattacac ctatttttgg atgtttcatt tgtcggattg cttgcaccca atgattcgtt
    40921 tcggttttct tatctaaaaa ttttaagcga tgcataagga aaattgcagt gatgttacca
    40981 agagcattac ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct caaaccagca
    41041 atgactccaa ctactgaagt aggaatacct ggaacagcac acatcaccgc tgtcaatacg
    41101 atcaagagta tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag ctctgcctta
    41161 ctcgctctcg gatttaaaaa taattggatg tctgtctgat attctaaata caaccgatag
    41221 cccactacca gtatcagaat gattcccaac gagatcaata tcaatcggat tattttcttt
    41281 ttcatatttc ctcctgaaaa aaatctatat tcattaaata atagtttaac ataaaaacaa
    41341 aaaagcaccg aaatccaaca taggatctca gtgcttttaa atagtaaaaa ttatttaaca
    41401 gttacagatg cgccaacttc ttcaagttta gctttcaatt cttcagcttc ttctttagat
    41461 acgccttctt taactggtgc aggagcgcca tcaactacag ctttagcttc tttcaagcct
    41521 aaaccagttg cttcacgaac tgctttgata actttaactt tttggtctcc agctgcagtt
    41581 aattctacag tgaattctgt ttgttcttca gcagcagcag cgccaccagc agcagctaca
    41641 gctacaggag cagcagcaga tacgccaaat tcttcttcaa tagctttaac taggtcgtta
    41701 agttctagga tagttgcttc ttttaactca gcaacgatgt tttcaatgtt caatgccatt
    41761 ataatttcct ccgtttaatt gttctttttt atttaatgct ttatgcggca attaagctgc
    41821 atctccatct tctttgtctg ccactgcttt gacagcgtaa gccacgttgc ggactggcgc
    41881 ttgtagtaca gatagcaaca tagatagaag tccttcgcga tttggcaatt ttgccaatgc
    41941 tgtgatttct tccaatgaag agactttacc ttcgatgata ccgcctttga tttctaatgc
    42001 ttttgcttct ttagcaaatt cgtccatgat tttcgctggt gcgactacat cttcgttact
    42061 gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc cagctttttt
    42121 agctgcacgt gaaagaatag aatttttgat aactttcatt tctacgttag catcacgaag
    42181 ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa cgactactac
    42241 tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg attgctgctt tttttgcgat
    42301 aattgcttca ctcatttttg agtttcacct cctgaatttt gatggaagag ttttttgcac
    42361 tgaaaaggca agtcactttt atagttactt tgctcaacca gtattattca tcacctattc
    42421 acttcgttca tagtgattct actcgctttg ctcgaaagga aactcctttc gctttgctca
    42481 accagtattg ttcatcatct cttcacttcg ttcatagtga ttcacaataa aaaactctat
    42541 gccaccgcag acatagaggg actattgaat atattcaata aacgtcctcg gtaggaaatt
    42601 aaggcttgat gccacctact gtcttcggta caagttaatt attaacctca actagcttag
    42661 catgctaaac tagttgagtc aagaattatt tttcagttgg atcaatcaac attctaatat
    42721 ttaattagaa agaagcttgg tcaacatgga taccaggtcc aaatgttgtt gtgatcgtta
    42781 catttttcat gtattgacct ttagctgctg atggtttagc ttttagcaat acatcatgga
    42841 ttgctgcaaa gttttcaact aatttttcat cgtcaaatga aacttttccg attggtacat
    42901 ggatatttcc agctttgtcc acacggtaag ttactttacc agctttcact tcgtttactg
    42961 cttttgttac gtccattgta actgtaccag ttttagggtt tggcattaag ccttttggtc
    43021 ctaatactcg tcctagacga cctacagtag ccatcatatc aggtgttgca acaactacat
    43081 caaagtcaaa ccatccgttt tggatttttt gaaccatgtc gtcgtcacct acgaaatctg
    43141 caccagctgc ttcagcttct tttgctttgt ctcctttagc aaaaactaaa acagtttgtg
    43201 ttttaccagt tccgtttggt aatacaacag caccacggat ttgttggtct gcttttttag
    43261 ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc aaattttgca atgtttgttt
    43321 cttttgctaa agctactgct tcggcaacag gataaacttt acttgaatca acttttttta
    43381 atgcatcttg catttttttg ctctttttag ccattttggt tcctccttga ttgtggttat
    43441 aacggttgta cctcccacgt gttctatatc tttcaatata gagccaactg agaagctgct
    43501 gtcttagggt gtatagatta ttctacagtg atccccatgc ttcgtgcagt tccttcaacc
    43561 atgcgcattg ctgcttcaac gttagctgcg tttaggtctt ccatttttaa ttcagcgatt
    43621 tctcttactt gatcgctaga aactttcgca actttatttt tgtttggttc gcctgaacct
    43681 ttttcgattt tagctgcttt tttcaataga acagcagctg gcggtgtttt tgtaacgaat
    43741 gtgaatgaac ggtcttcgta tacagaaata acaaccggaa tgatcaaacc tgcttgatca
    43801 gctgtacgag cgttgaattc ttttgtgaat cccatgatat tgatacccgc ttgacctagt
    43861 gcaggaccta ctggtggagc tggagttgct ttacctgcag gaatttgcaa tttaacgatt
    43921 ttttctactt tctttgccac gagacatacc tccttaagtc cgtgatgtgg ttaattggag
    43981 atgcatattt ctcctcccac gttttatctg atctgaaaaa atccagacaa ataatgtgca
    44041 aaacaatgca cactggtaca gtatagcaaa tttatttgtt ttttcaagat ttttttctgc
    44101 tatttttaca ttttgcttga ataaacgcta aatttattct aaaaaaacaa caatttttcg
    44161 ttctcatttc ttcttttatg ttacacttgc gctagcatgg ttcaacagat gaaagcaaac
    44221 aataacacgg ttttttagac ccttgtacaa agattcctac ataacaaaat gaggtggaga
    44281 aaatgaaaga tttaaggtat agaagtgtct ttgatattat tggacctgtg atgattggtc
    44341 caagtagctc tcacactgcc ggagctgcta gaatcgggaa aatcatacgt agtatatttg
    44401 gcgaacagcc tgattctgta gatatttatc tatatgaatc atttgcaaaa acctatcggg
    44461 ggcacggaac agatattgca ttagttggcg gcctgttagg gatggaaccc gatgatgaac
    44521 gtttagctga ttctttgaaa ttggcatatg aagccggtat ggaagtcttg tttgttccta
    44581 agagtgaaaa agccgaacat ccaaactctg taaagatgct tctaaaaaaa ggaacacgaa
    44641 agctttcagt cactggcatt tcgatcggcg gtggcaatat ccagatttct gaattgaatg
    44701 gattcaaaat ttctttaagc atgggtactc ctaccttgat cgttgtccac caagatgttc
    44761 caggtatgat tgctaaagtg actagtatac tttctgagac caatacgaat atcggcacta
    44821 tgacagtgac acgggaatca aaaggtgaaa atgccattat gataattgaa gtagatgatc
    44881 ctcaagtaga agaaacggta aaaagactca aacagctgcc aaatatcgac agcgtaaatt
    44941 atttcgatta aggagctaaa aaaatgtttt tatcgattga agaattagta gatcaagcaa
    45001 aagcttatcc aagtgtagca gaattgatga ttgcagttga aatggaaatg agtggccgtt
    45061 cccgtagtca catcatcgag atcatggaaa aaaacctctc agtaatggaa caatcaatca
    45121 acgagggaaa agaaggtgtt acctccgtca caggaattac cggtggtgat gcaaaaaaat
    45181 tagatgatta tttaaatgag gcagactttt taagtggaga aacaatctta aaagctgtac
    45241 gcaatgctgt agctgttaat gaagtcaatg caaaaatggg gttgatttgt gctacaccga
    45301 ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat cggcttgatt
    45361 tgacacatga acagcaattg gaattcttat ttaccgctgg cgctttcggg ttagtcatcg
    45421 caaacaacgc ttctatcagt ggtgcagaag ggggatgtca agcagaagtt ggttctgcaa
    45481 gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg gactcctcat caagcagcac
    45541 aagcagtagc cattacattg aaaaatatga tgggactgat ttgcgatccc gttgcaggct
    45601 tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct tttatctctg
    45661 ctgatatggc attagcgggt atcgaaagtg tcattccgcc ggatgaagtc gttgctgcaa
    45721 tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa ggtggcttag
    45781 ctgttacgcc aactgctaaa cgacttgcgg aagagatttt aaataagagg gcgtgataaa
    45841 gctccttaat ttcgagtatt aagagattag ttatcttcat aacaaaaaac gaagagagga
    45901 ctaattgccc ttctcttcgt tttttattaa tccatcaatt tcgtatattt tacttcatcg
    45961 atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag gtgttcaaaa
    46021 acagatgtta ctccttctgc cccacctaga ttcaacccat aaatcactgg tcggccaatc
    46081 gcaactaagt cagcaccact tgccagagct ttgaagacat gctctcctcg acggacacca
    46141 gagtcgaaaa tcactggtac acgtttgttg actacttcgg caatttttgg caatacttca
    46201 aaggacgcag gacctccatc tagttgacgt ccaccatgat tggataccca gataccatct
    46261 gcccctgcag agatagcgat tactgcatct tctggcgatt gtattccttt tacgataact
    46321 ggaagatggg tgatttcttt gattgttttg atgtcatctg gtgtcaagcc ttgttttgca
    46381 gctgcataaa tttctgcgat tccttttcct tcgccattac cacttgcact ttgttcgcta
    46441 tacgcagcta agttaggcat tggtaatgga aattggaatt gattgatgac gtcttcttca
    46501 cggtaaccac cgagcgttga atctgccgtt aaaatgatag ctttagcccc tgcttctact
    46561 gctttgttca aaatgaactc attgaatctg tcgtctttac tcatatataa ttggaaaaac
    46621 tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg tcgtatttgc ataagtgctg
    46681 atggaaaaga ttgatccagc agcagctact cctttagctg tatcagcttc ccctttttca
    46741 tgtgccaatc cttgggccgc tgatggcgct tggataatcg gtgtatctaa ttcaattccg
    46801 aaaacagatg tatgcaaatc ggctgagtcg attccgcgta atatccgtgg catgattttc
    46861 tttgtattaa aagaagtcgt attttctttc atcgtccact catcttcaga accaccgcga
    46921 atatagccaa aagctcctgc ttccatgcgc tcttttacac gaccttctag ttaggcaatg
    46981 tttacaatat cgattggatg ttcttcatta cttgtatgat aagtcatagt agatccttct
    47041 ttcatcttct tgattacata taaatcatac taaacttaca aaaagtaagc aaacatttac
    47101 tctcagatac ttttatcgct attctttcca aataaatcga cttacaagct gatctacttc
    47161 ttggttttca tgaagcatac tatgagaggc attttcacct gtcactactt tttcctctat
    47221 ttcatttccg cgttgcttca acaacgcata gaccgctaat gcactgttta acggtactgt
    47281 tccatcactc aagtctgttt catctagttg accagcaatc aagaaaaatc gagtggttat
    47341 aggaatatta ccgatcattt gctggtaatc ttgatagcgg ttagaaacga cagcaggccc
    47401 ctttgctaac tcatctgtca gagactgcgc actatcatcg acaaaatcat taaaaggcgc
    47461 gccaattgct gttaatgtct gaacttgtgg caatgacgta tcctgtccat acgttcctaa
    47521 atagcgtagt gcactaactc ctcccatgga atgccccact atgttgactt tatctatgcc
    47581 gtagttttct ttcagataca aaagacaagt tttgatccat tctgcttgat tccactcgtt
    47641 atttttatta tcttcaaaca atacttggac catcggattg tctgcttttc cagacaaatc
    47701 tcctgttgct tgtatttgtc catctgttcc tactgttaaa accaattctt ttactgcttg
    47761 ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc cgctgtagcc
    47821 gtggaaaaaa agtgttggaa tagtggtttt ttctatcact ttactggatt ctgccgtttt
    47881 ttctttatca gaaacttcag tagtatttat cttcgtcgtc tggcaaccac ttaacgcaaa
    47941 caaactgata aggactaaag ctgctgcagt tgcacgtttg cataaatttt tgattggttc
    48001 tgtcatacac ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc caatcatatg
    48061 atcgacattt caagtctctt tattaatcga ttttatctac ttgatcgaaa tcaagttctg
    48121 tactggtttc acgtccaaac atatcgatgt tgactttcaa tttttctttt tctccgtcaa
    48181 cttctgttac ttgtccttct aatccagcaa atgcgccttc gatgattttc acgacttcgc
    48241 caacttctac ttctagatca ttttgtcgca ggctcatacc gatagaacgc aagatatgat
    48301 tgacttcttc tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa ccaacgaatc
    48361 ctgtcacacc aggtgtgttg cggacaacat accatgaagc gtctgtcatg atcatttcaa
    48421 caagtacgta tcctgggaat gttttatgaa cgatttcttt ttctttccca tttttgactt
    48481 cagtttcttt ttcttcagga acaacgacac ggaaaataaa gtcggccatt ttcatacttt
    48541 gcgcacgaga ttcgatattt gcttttactt tgttttcata tcctgaatat gtatgcaaga
    48601 cataccagtt tttttcaaaa gattccattg aaggctcctc atttcattgt aaaataaaaa
    48661 aacctcagta cacccgaagt ttttctttcg cttcattata gcactaaaag tacgttgtgg
    48721 ctagcgagtt attttaaaat ccagccaaat aagctttgga tggctgtatc cattacgtaa
    48781 aacatcaatc cgaaaataat agaagtttcg atgactacta acgtatcttt acggagttgt
    48841 tttctgcttg gccaagttac ttgtttgatt tcatctttta cgctttttag aaatttcatg
    48901 agacaacctc ccttgctact gtttatttcg tttctttatg taatgtatat tgattacaat
    48961 atttacaaaa cttattgatt tccaaacgtt cgccgctttt tccttcactg actgattttg
    49021 tgtagttgcg agacccacaa acggaacaag cgagtgctgc ttttttcttt gccatttact
    49081 tctgccccct tgctgagaat cagacttata cttcataaca gtatcacgta actttttact
    49141 tgtcaatttg tgaaataaac tatctattgt tttggataaa attatgatat gattaatttg
    49201 aaattaagct aaaatgagac aattggaggt atagcgatgc tattaaaaac acttgtatat
    49261 aagaaaaaag atctgacaac cgttacagag actagtacat tagaagaagc attaaaaatt
    49321 ttagaaactt ccggataccg ttgtgtgccg atcttagatt cttcaggtaa tattttccgt
    49381 ggaaacattt acaaaatgca tatctatcgt cataaagcaa atggcggtga tatgacgctt
    49441 cctgtcactc atcttttgaa aaacgctaca aagttcattt acctggatac ttctttcttc
    49501 aaagtattct ttacgatcaa agagcttcct tatattgctg tccttgacag tgaaaatcat
    49561 ttttatggta ttttgacaca tagtacgttg cttaatatgc tctctcaatc ttggagcgta
    49621 gaacaaggaa gctatgtact gacaattgct tccacaggaa agcaaggaga tttggctgct
    49681 attgcaaaaa tcatttcaaa acacagcagc attgccagct gtattactct tgatatcgaa
    49741 aaagacgaat acattcgacg tacattgatc acgctaccgg ctaacactga agcgaaagta
    49801 tgtgaaacga tcgtcaacca cttagagaaa aagaattata aagtggtaga aattgaaaat
    49861 ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa aacagctaat ctgctttctt
    49921 ttttcgcttt ttataattag acacgctttt caaaatcaag cgcgcgatct aaataataca
    49981 tcaatggtgc tgaaacagcc atgacgataa actcacttaa tgcaagcgtg ccataggttg
    50041 tccaaaatgc ttggccacct gttggaacta acatataagc aatcagtccc atgctgatgg
    50101 taaagaagat cgtgttcaat actaaacgca tctttacatt tttcactttg ttttgcaata
    50161 aagctgtcag gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca tccatgatac
    50221 caaaaccaaa gaaggcatta tagacgatga ccccgcctaa aactccccaa agcaatttac
    50281 ggttaaagac aactaaatga ttcaaacttt ctgaaatacg aaactggatc ggccctgagg
    50341 atacaggcgc aactaaaata gttaacgcta gataaagagc catgatgatg ccatttacaa
    50401 cgattacgga taaccgttgt ctgcttttcg ttttttgcat tttctctcct cttttctcct
    50461 agttttttta acgcgggatg gttgatgaac cgcggttatg tgaaacaaac aataattata
    50521 acagactttt catttatttc aatccgtctt atctgctttt aaaaaagcaa aagcttaaac
    50581 aatgtaaacg tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa ggggtcataa
    50641 gaattcccag taaagcagac gaagtaggat ctgcaagaac agaattgttg tgaaacttaa
    50701 ataaataaaa aaggatgtgg gaatttgagc aacctcttac tagagaacgg tctagcaagc
    50761 agctgaagat ctaagtggac atgccgaacc ttggaacttc tccttacgta agtaatggat
    50821 catgcgcttg ctaggctttt ttgtgtttct cggtcttcgg acctttttca ttttcctaga
    50881 cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa aacaagtttc
    50941 tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa caggagtatt
    51001 aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta tcatatattg
    51061 atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat tttaccggta
    51121 ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg acacgatgct
    51181 gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga agaacagaaa
    51241 acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt acatcaggta
    51301 cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc agcaggttct
    51361 ggagatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg acttcagctg
    51421 attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga aaaaataagt
    51481 gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc tatcgtccat
    51541 gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg atcattttcg
    51601 tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat tctggtagat
    51661 gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac gaagcatcct
    51721 ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc agtcattgat
    51781 tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct tttacttata
    51841 cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag attacgtatc
    51901 gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa acgacctttt
    51961 gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag gttttgtcag
    52021 aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac ttttgtcaca
    52081 gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg atagtttctt
    52141 tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac taagacacaa
    52201 ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc actgatcaag
    52261 ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct aaccatttct
    52321 ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt gataaaaata
    52381 tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca agccgaaaag
    52441 ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac cgttatatcc
    52501 cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa caacagcagc
    52561 gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt aatgggcgca
    52621 ctctggtttc gtttactgat ctctttgcga ttaaatgcta gattgatgac gctgaaaccg
    52681 ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc cgtcaacagc
    52741 tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac cgagagtgcc
    52801 atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa cagaatcagt
    52861 gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc atctgggcga
    52921 ctctgtatct tgatcgtcgg catctttcct acttcgactt gttcggtaaa gtctgcaggc
    52981 agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc tttttgtgag
    53041 gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag gccatcgact
    53101 acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga ggtattttca
    53161 acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac acctatcagt
    53221 aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt cataacgcac
    53281 ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga tcaaccatag
    53341 aagattggta taaaaactag ccaaatcagt gtagaaataa agtttataca aactttcgct
    53401 gatcaagttg acgagattta attgaccaac aatcggaata tgaagatcaa tccaatactt
    53461 cacttgttcc gaccccatca taccagcaaa aaagctcatc gcaatcgtca ctgaaatcaa
    53521 gatgctatcc ttttgcacca gattcatttt tggtatcaaa ttaccaacca atgtacctaa
    53581 caaaatcgca cacaacgacc ccagagcggt aacaatcatc aaccattgcc aacgttcacc
    53641 aaagtctact tgatagataa aacgaaagac agctaacata atataaatct ctgtcaaaac
    53701 aatcgtaaag cttgctgcca aattagcgat tgaaactaac gattgctttc taggaatcaa
    53761 ggacaagcga ataccatttg acgattgatt tgcttgctga tcctgtacat tgcggacccc
    53821 ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg aagaaataaa aacttttgaa
    53881 actcatatta cgacctgcat gaacttcttg aaaagatact tcttgttcaa aaacagtcat
    53941 gatgggcgcg agatcaacgt tctccacttc tgccacttta ctcgaaatta taccaatacg
    54001 ctgtaaaaat tgatccatga cgtttttcat caacgtctga ctcattcctg cttgacccac
    54061 atggagcgaa acagatgaac catcaatgag ataaaaacca gccaaatcat ctgcatttag
    54121 cttttcattc gcttctactt ccgatagttc ctgtacttca aataatggtt tatcattgac
    54181 ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc gctgtttgat ccgctttgat
    54241 tacaattcca gtattaatcg tttcgagatt gccaccttga tcaatctcgc caaaggccgc
    54301 cataaacatc aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca tggatttatt
    54361 tcgcagcagt atttttgtgc gatatttata aaaatgccaa aacattattt cccctcctct
    54421 agtctctcaa atccttgcca gtaatctcta aaaagacatc attcaaggac gcttctttgg
    54481 ttgctatttt tccgtaactg atcttttctt cttgtaagat tgtaagaatc ggcaacaata
    54541 ctgtatttag ttcagtagtt gttacttgga gctgaccttt tttataagcg attccatcag
    54601 aagctattgt tcgctgacaa cacgttttct gggagtaatg ggatgtcgat cgtcaaacga
    54661 tccgccgtcc gtaccatatc ttttagagat tctttggttc ctttagcaat cacctgtcct
    54721 tgatccatga tcacgatctg gtcgcaaaga atttctactt cttccatgta atgtgttgta
    54781 tagacaatgg ttgctccttg tcgattcagc tccttgatac tttctaaaat tttattccga
    54841 ctttgggggt ctacagctac tgtcggctca tctaaaaaaa ttaaccgcgg cttgtgggca
    54901 atcccacatg cgatgttcaa ccgacgtttc aagccaccac taagttctcc tggacgaaac
    54961 gaaacatatt tttctaatcc cactaattga atcacctctt caatcaaggc tttccttttc
    55021 tctttttcgg aaacatataa accgcaaaaa taatcgatat tttcttgcac acttagttcg
    55081 tcaaagaccg caacgtcttg aggaacgacc ccaatcattg ccttgacagc ataattttca
    55141 ggagtcattg ttgtcccgaa caatttaatg gttcctttgt cataatttaa caaagaaagc
    55201 atacagttga tggcagtcga tttcccactg ccgtttggtc ctaacagacc taagacttct
    55261 ccttcttgta cttgcagatc aaaatgattc aatgctacaa aatctccata gcgtttgact
    55321 aatttatcaa tttcgataat catcatgaaa aactccttcc aaatttcttg ttgatctgac
    55381 tataataaag tgcaagaaaa gaaactagtg acgattggct aaaccacaca tgacatttgt
    55441 cacaatgaag gagtctgcta tgcataaatc tcggctactc aacgaaacta gctttctact
    55501 ggttatctca ttgcttttgc tggtaagtca aggattcgtt gctatgcaag ttatctattt
    55561 attgatcgcc gttttattca ctggtgtcag ttatttactg cctacaaagc caaattggat
    55621 tttccaagtt attctatttc ttttatgtgc ctttttacct gatcttgtcc tgtttctgcc
    55681 agcgacgatg cgagtgatcg cccctgaaga aagccacctt gattacttgt ttttgttagg
    55741 ttttttgttg atttcgacgg ttggtaatca tccccccatc aaggtcgcct ttgtcgtaat
    55801 tttgctgttt ttttcctttt acttacgaaa aaaagatcaa cgaacgaccg atcttgagaa
    55861 aaagtacatt gtctccaaag atactcattg ggaaaaacaa caagagttat cggaacaaaa
    55921 taaagcattg gccctgtcac aagaaacaca attagccttg caaattgtgg aagaacggaa
    55981 ccgaattgcc cgagacatcc atgacaatgt cggtcatctg ctgtcaagtg cgattttaca
    56041 aattggtgcg cttgaaatga tcaatcaaga accaaaacta aaagaaccat tagaaaattt
    56101 aagtgccacc gttcacactg ggatggatcg gattcgtcaa agtgtccatg acctccatga
    56161 gacatcgatc tcttttcaac actccttaga gtttctgatc gctgattttc cttcttctgt
    56221 tacgattgaa ggaaacttgt ttgaaggatt gaatgaaacg cagcaaaact gcttcttaat
    56281 ggtcatcaaa gaagccttaa ccaacagtat caaacatagt catgccaaaa ccgttgtttt
    56341 acattttcga acgttgccgg ccttttatcg cttgcaaatc acgaatgatg gcaccagccc
    56401 acaaaaaaat tctgctcccg gtattggttt gaaaacgatg cgtcaacgtg ttcaagaaca
    56461 aaagggacaa gtccatattt tccaagaaaa ccagcaattt cagttaacga tcattttacc
    56521 aagggaggga caccaatgat aacagtagtc attattgaga tgatccattt gtgacgaaat
    56581 cgttacaaac cattttagaa agcacgaaag agatccgtgt gttaggcatt ggtcattgtg
    56641 ccaaggatgc gctagtctta tacgaaaccc atcgtcccga tgttttgctg acggacatcc
    56701 gcatgcctaa acaaacgggc atcgatgctg ccaaagaaat tttggctaaa tttcccaaag
    56761 ccatcctttt gcttttgacg acattcaaag atgaagaata catccgcgaa gcattttcca
    56821 tcggtgtcaa agggtattta atcaaacaga acttgcaagc gattattcct tctgtcaaat
    56881 ccgcttataa tggacaggcc gtatttggta atgaaatcat cgagactttc actcaaatga
    56941 tgaagaataa accgacgatt gatagtcatg cttatgcttc ttttagtgaa cgtgagcttg
    57001 caatcatcaa agaagtagct gccgggaaaa acaataaaga aattgcagat gccttgtact
    57061 tgagtgacgg cactgttcgc aattacatca gccaattact agaaaagcta gatttacggg
    57121 atcgcacgca attggctatc tatttttatc agcatttgca ataagtctta tctaacacaa
    57181 aaaaacctga atgatgctcc ttcgcatcat tcaggttttt ttcacatctt ctattcaatt
    57241 gtcccgcaaa ccatttcctc acgattgaaa gaatcaagga ttcgcagggc tgccaggatt
    57301 cgttggattg ttcggcgtgt taggcggaaa aataggcgcc ttgtctaacg ttttttctag
    57361 ttttgattct tcacgatcgt ctcgttctac atcaggactt tcattcaatt tgaattcttt
    57421 ttccatctca tctaactttt taaagtcatt gccagattct tttgctaatt tcgctaattt
    57481 atcttcggtt tctttagcca taaagtaacg cccccctttt gatttcagct tagcatatca
    57541 cactgaaaaa acaaaagaaa acgctaacca acgatcattt ttagctattt ctcatcttta
    57601 aaatcaagca ttttttgccg gtataaaatc gtagggagaa ctgttttcca taccaatcat
    57661 cggatctact aactggttat cgatcacttc tgctgtaaga acgaaatctt tagaaacagc
    57721 aggttctacc tcttctttct tttcttctcc aacaaatgaa gaaaacagat ctggttccac
    57781 tgtttccatt acttggtttt tcgtttcatt cgtcccactg tcatcttcta caggtagctt
    57841 ttcttttaca acccgtggct cttttgtata gttcccatct tcaaatggat catttccgac
    57901 caaagaatct tcataagctg ctagtttggt ccggagagtg atattcattt tatcattatc
    57961 catgatcctt gatttcaacg tagtcatccg gcttgctgat tcattcgcta cacaagtgac
    58021 ataggcttgc tcttccccta acaaaatcaa cagttcctta ctgcgagtca ctgccgtgta
    58081 gagcaaatta cgctgaagca tccgctgata ttgatggacc atgggcaaaa taaccatctt
    58141 gaattcactg ccttgtgatt tatggattga gcaacaataa gaaagagtga ttttattcca
    58201 ttcgtttctt ttataagtga cctcatttgc atcaaactgg ataactaatt catccacttt
    58261 atcttcagaa tcttttgcca agatgatacc cgtgatttct cccatatctc cattgaatac
    58321 attcaattcg ggagtattga ctaattggag gactttgtct cctatccgat aaacggtgtc
    58381 gttccaatgt acttcctttt ttccaggttt ccctggattg aatatctcct gcatcatttt
    58441 atttaacgca tcgatccctg caggtccccg atacattggt gctaatactt ggatatcttg
    58501 tgcacggaat cccttatttt tcgctttttc cacaatttta gagatcaagg gttcaatttg
    58561 ataagcatca cttcggaaat aggaacgatc tttttgattc tttgtgaaat cttttggcag
    58621 acgtccttct ttgatctcat gggcaagagg aataatacta gaaccatcac cttgccgata
    58681 aatctgattg agttccattt tgggaatttg ttcgatttcc aaaagatcat ggagtacttg
    58741 accaggaccg acagagggaa gctgatcttt gtctcctact aagataactt gcatattcgt
    58801 cggaatcgct ttgaataatg tgttagccag ccatgtatcg accatagaca tctcatcaac
    58861 aatcaaaagt ccgccttcta attctttcgt actgactgaa ggtgtttttt cccgtccatt
    58921 cagccccaga agacgatgga tcgtactggc aggcagacct gtcgtttcgt tcattcgttt
    58981 agctgctcgg ccagttggtg ctgcaagtag aataggaaaa atttcttggg tatattttga
    59041 aggatccaat tccagtccgt tcaactctgc aaaaagattg acgattccgt tgatcacagt
    59101 agttttcccc gtacctggtc ctcctgttaa aataaataga ggggaacgga ttgcttcagc
    59161 gatagcttgt tcttgcgaag gtccatactg tatgccgaat attttttcga tacgccgaat
    59221 attcttctgc acttcttttt caggataatg gatttctttt ttacgactga gaaggcgctg
    59281 gatcgaatta ccgatccccc attcagaaaa gtacaaactg ttttcgtaaa gattagtctc
    59341 ttcttgttgt atctttcctt cttcaacaag ttggataatt acagtagcaa cctgatcagg
    59401 ctggatttca attgggcgac ttgtttctaa aatacggatc gtttgctgaa gcaatgtctc
    59461 cgccggtacg tatgtatctc ctgtttgaac tgcatgctga aaaatctgat gcaagatagc
    59521 tgctcgaatt cttctcggtg aatctgctgc aataccgatt tgttcggcta gattatctgc
    59581 acgtttgaac ccgattcctt ctatgtcttc gactagctgg tacggatttt cttcgatgat
    59641 atccaaagac tcgttcttat aggcctggta gatactgaaa gctaactgac taccgaaccc
    59701 gtaacgattc agtcccatga tcacttgatc catcccgtgg tttagccgaa tcgtctcagc
    59761 aagcatttct ctttttggct tcgtcaaacc ggagatttgt tttaataccg aaggatcagc
    59821 aatgattttt tcgatagcat cttctcccag caattctaca attttttctg ctgttttctt
    59881 cccgattcct gggaattttt cgctggaaag atacgctacg atcccatttg cagttgttgg
    59941 cttttcttgc tcatatcttt cgagtttaag ctgccttccg tatttaggat gatccactaa
    60001 ttctccataa aaacgataag tctcattttc ttgaatatcg ccgaaacttc cggtgactac
    60061 aatctctttt tcacggtaat cagctgtggt ttcactgatg tgtaccaaaa gcactttgta
    60121 aaaattacta ggattttgaa aaaaaatcgc ggcaactgta ccaacgaaat aaggcatttc
    60181 ttctggctgc atcggaagcc gcctccttct tctatagtaa aggttctggt gctgtttcag
    60241 ttcctaaaaa atctgtttgg ttccatactt tcagttcata tggcaataat ctgcaaggct
    60301 cttcagtctc taaaatcgtc aaactactat tgaacaatcc acccatttca cgcaactcac
    60361 ttagctcttt tcctgtcatc cattgaatgg ctgcagttaa agaagctcca tgtcctacga
    60421 ataaaagtgg gccatcttta tgttgggcaa ccgcatctgc tacaacagtt tcgatacgtg
    60481 tgattgccga ttcaatcgtt tctccgtgaa aaggagaagg atcatacaga tctaaatgat
    60541 tacgcaaatt cggcagatta tccggatatt tctggtacat ttcatcgatc gactgacctt
    60601 ctaaagtacc cagccctagt tctcttagct tatctgtata aaaaatctct accggacgag
    60661 caagctcttt gttgatacct tctgctgtat cacgtgctcg ctttgctgta ctggaataaa
    60721 ttgctttaaa aggaacatat tttacctttt tcccaaatgc tttgatttct tcgtaacttt
    60781 gtggcaaaag cggagaatcg ccattgctcc cttgaaaacg tctttcctga ttccattgtg
    60841 tcttcccatg tcgggtaaaa tatagttcca tacgcagcaa actcctgtct ttcttttatt
    60901 ttactctata ataaactgtc taatccagct tgtcattttc atagtgatgc gtcaattcta
    60961 aattagggaa tccttgctgg cgaagtgctt catacactat cattgcagct gtattcgata
    61021 aattcaatga gcggacatgc tcatcattca tagggatgcg taaacacttt tcttcgtttt
    61081 cacgcataaa tgtttctggc aaaccggttg tttcttttcc aaataaaaag aagtgctctt
    61141 ctccatcagc aaaattttcg tcactatata ctttatgggc aaattttgag atcaaatgta
    61201 gtttacgatt acctaaaaat gttaaaaaag cttctaaatt actgtggtaa gtaatattta
    61261 catcattcca ataatccaac cctgcccgtt ttagatgttt gtcatcagtt gaaaaaccta
    61321 gtggctcgat taaatgaagc ggagtattcg tggctgcaca cgtacgggca atatttccag
    61381 tgtttgcagg gattaatggt tcaaataaaa cgatatgatt catcaggatc attcctctca
    61441 tatttatgtt cgtttctatt ttctgacaat ttgatttttg cataaaaaaa aacgtatcaa
    61501 ctcggtgtca gtcactgcga gtcgttacgt tagtgaagct cttctcacta acttttatca
    61561 gaacaggaac ctgataaaaa ccaatgaaaa aacaacttgt tgctaatata acaccctcaa
    61621 cttttttttg caacctctac tcattaaaaa ctgctagaaa aacgttttct cttagttcct
    61681 tttaataatt ctatgtcaat tgtaatcttc ttcaaaggat tcttatataa gtctgctttg
    61741 attcgctctt ctacctgcca ttctaaaggt gacatggcca aaagatcttt ttgattttcc
    61801 tctggtatct caaattcata ggttacacgc tgacgttcag ttaatttcat tgcttcagca
    61861 aacttggcta ctacacgttc attggaataa ctctgttttt caggttgatt cggataaaaa
    61921 gcctgacgta gttcctttaa ataattttct tgtggcacca ccttcaacac ttctccatct
    61981 tctgagagta cacgttggaa ttcttggtaa tgagaaggcg aaaagatatt taaaaccgcg
    62041 ttgaatgttt gatcagcaaa cggcaaattg gtcaaatcag ctacacacca aaatgcttcg
    62101 gcagattgtt ctgtagctgc gtagactcct tcttttgaaa tatcaaatcc aacagcattt
    62161 tgaatctttt tcaattttga aagttcattc aagaagctac cttctccgca gccaacatct
    62221 aatagcgaga cttcttcagg taaatgctgc gcaatagtct caataacggg ctgatacatg
    62281 ccagattgga tcattctacg ccttggaacg aacattcctt gatcgtattc tgttttaacc
    62341 gcatgggata aaaaataaag tgttcctttt ttggaaaggt caaaacggtg attatttttg
    62401 catatcaagc cattatcaga cttttttact tcttcttggc aaaccggaca gcggaacagc
    62461 tgatgatttt ctgccaaaaa ctggcgtcct cggtcaattt tttttaacat agttccccct
    62521 catttctgtt ttatcttacc gttagatgct cactttggca actgctaaaa atatgataca
    62581 atgcctagaa gaaagtgaaa gataaaggag aagattagat gatcaaagaa ttttgcgctg
    62641 aaaactacac atctattcct ctagcaattg ctagaggagc cgatcggatc gaattgtgtg
    62701 acaacttagc ggtcggtggc acaaccccga gtactggtgt gatcgaagaa acgttaagtt
    62761 atgctgggga aaaagatatc cccgtaatga cgattattcg tcctagaggg ggcgacttcg
    62821 tctataacga tatcgaattg aaaatcatgc atactgattt gattgaagca aagaaattag
    62881 ggacggatgg cgtagtattt ggttgtctta ctccttcagg ctggctagac gaagaggcct
    62941 tagaactatt gatcgaaaca gcagaagggt tgcagatcac cttccatatg gcttttgaca
    63001 gtattcctga agatcggcaa ttcgaagcaa tcgattggct ggtagaacat ggtgttgacc
    63061 gtatcttgac tcatggcggt cctgctgaca aaactattgt agaaaatttc ccacggctta
    63121 aagaattgat tgcctataca gatggccgct taatcatttt gcctggtggt ggtgtcactg
    63181 cagagaatgc agcacatgta gctaaagaat tgaacgtaag cgaagtacac ggaaccaaaa
    63241 tcgtggatct tcagttataa tcttataaac aaaaaaacat ttgcaacagg cataatgctt
    63301 attgcaaatg tttttgtttt tttacataaa atagcccata aataaagaag caatattgaa
    63361 ataaatcagt acactcgtca aatcgctcaa agtcgtgata aagggaccgc ttgctactgc
    63421 tggatcaaat cctagacgat ccattagcat cgggattaaa ctacctgcaa gattcgccac
    63481 tgtgattgca aatagcatag ccatccctat gacaaacccc aagataaaat tatgtttcca
    63541 aaaacctacg attagaaaaa tcgtgatccc cgtaacagct cctgtgacaa cgccggtcaa
    63601 gacttcagcc aaaacaagtc gaacaaatcc atgatctttc tcatcagaaa tcgcaagtcg
    63661 acgaacagcc acagctagcg actgggttcc agcattaccg gcagttcctg taatcagcga
    63721 aatgaagact gctaaaatac tcgcttcact taccaagtct tcatagtgac tgattaaaga
    63781 agcggtcgac atacctaaaa acaacagagt gatcagccaa ggcaaacgtc gagacgctgc
    63841 ctttactgga ttttcattaa tttcttccac attgaccccg gcaagtcctg aataatcgct
    63901 agcagcttca tcatcaataa cgtcgatgat atcatcgact gtgacgattc ctaacaaatg
    63961 atcatcataa tcggtaacag gaagagctaa gaagtcataa tctctaaatg tttgtgccac
    64021 atcttcttgg tcgtcgccta catgaactga aagcactcgt tcgctcatca gatcagaaat
    64081 cattgcgtcg tcatcattca cgattaggtc gcgcagagaa ataaccccta ctagctgacc
    64141 ttcttgatta acgacataga tgtaatagat tgtttcagct acatctgctt cgttcttcag
    64201 cacgtacatc gcagagcgaa cagtttgatt ggctacgatc gaaacaaatt cagttgtcat
    64261 gatcgcgcca gccgtttcat cctcgtaatg gagaagttcc ttgatttcac tggcatcgtc
    64321 tgccgacatc aaacttaaat atttagcaat ttgcttttta tccagcgtat taagcaagtc
    64381 aacagcatta tctgtataca tctcagaaag catatcagct gcatagctag gacgcatttc
    64441 atctaggtat tctttcatat gttcgttgtc ttcttcaatc acatcgaaca tatccgctaa
    64501 ttctttaggc gacagatagg aatagatcag ctgcctgttc tcttctgtaa gagattggta
    64561 gaattgcccc tgctcataga tatgcatctc taaaaaactg tttctgaatg cctgaatatt
    64621 attttccctt aaatcttgag tcaactgggc aaatctttcc tctaattctt gattttcatc
    64681 caaaccaatc tcacctcgcc ttattcttca aaccgtctca caactgcctc catgtctgcg
    64741 gctaaaggct gcttgaactc cagcaactct tctgtaaatg gatggtaaaa acgcagatca
    64801 taacaatgaa gggcttgtct ttttattccc agatccattc gcccgccata catatcatca
    64861 ccgagcaggg cacagccaat tgcggaaaaa tgaactctta tttgatgtgt ccttcctgta
    64921 tgcagctgga tatcaactaa cgcgatttct gttagtcgct tctctatcca gtactcagtc
    64981 tgtgccgaac gtccttcatc agagaccatt cgcttcaata gcgaagaata atctcgggcg
    65041 atcggcaaat cgatatgtcc atgtgccgat aaatctgaaa gccttccact gactaaggcc
    65101 tgatattttt tcacaaactt cttttcacga agttgcatat ctaattttgc gtgcgcaaat
    65161 ccatgctttg caaaaagcat caaaccagat gtatcccgat ctagtcgcgt caccacatgg
    65221 attacttggt tttcgtaacc ttgtttttta taataagcct ttacacgatt agccattgtc
    65281 ccatttggat ggtattgggc aggaatagac gaaacgcctg ctggtttatt tactaccaga
    65341 acgtgttcat cttcatacac gatatcgatc ggtgtttcat ccaaaagtac ggtttcatgt
    65401 tctccttcag caggaatcac aatggttacc ttatcatcct tagctagaga aaacaagaca
    65461 ttttctactt gatcattaac tttgatttga ccgccttgaa attttatttt cgctaacaga
    65521 ccctttgaga tccctttttc ttttaaaaaa tattttactt gctgagaatg atctttctca
    65581 taatcccatg taaactgcat atcaatcctc actaataaag gcatctttca cacgatgcca
    65641 aaaatgcata tgtcgataag aagcaaaatg gatccgttct tctgctattc gataataaat
    65701 tgagcggata tttgcttttg cgacattcaa ttgatcaacc gttacaagat aatcttctgt
    65761 attttccaat tttatttcta gccattcatc atgggcaatc acgatcggag aacccaaagt
    65821 acgaaacact cgattattca gtgaagcaat ctctgccaac tgaaaggcat tgatactagg
    65881 atggatcaca gcaccgccga cacttttatt atatgctgtc gaaccagtag gcgtagaaat
    65941 cgtcaatcca tctcctctaa aactttcaaa cagctcgttt ttgatgtata catcggctac
    66001 catcgtacga ttggcccgtt taatcgttga ttcattcagt gctaaaaaat gctggtccgg
    66061 tgtttcatcc aaataactga tccgaacatc cagcaaggga taactaacac tttgttcccg
    66121 gttcgttcgt aagctgtcta ctagttcttc cagctcgtaa tcccgccaat ccgtgtaaaa
    66181 ccctaaatga cctgtatgca cgcccaagaa acgcacatca ttcagacaat gactgtagtg
    66241 atggaacgcc gacaataatg tgccgtctcc gccgactgag atgaccaatt ctggctcatg
    66301 atcatctcgt tgaatccctg cttgttctaa aagtgcgttc aaacgttctg tcacttctat
    66361 agtctttgct tctttattat gaacgatcgc tactttcatc ccctgcctcc tctctttcgc
    66421 tttctttctt caaaagcgtt cgatagtaga catcatctga ttttcctctt ccatgggaga
    66481 ataaccgctg tgcttcttgg atttcttcac ggatactgga catctcttca tccaactgat
    66541 aggcagcttc tgccgcacgc tgaagtcgtt cactcatttc ttctggaaaa acgccttgat
    66601 atttataatt cagcgaatgc tcaatcgttg cccagaaatt catcgcaaga gtccgaatct
    66661 ggatttccgc caaaattttc ttctcaccgc tgatcagctg tacaggatac tcgatcacca
    66721 gatgataaga acggtagcca cttgcttttt tatttgtgat gtaatctcgt tcttggagaa
    66781 ctttcatgtc ttttctttta cggatgatct caacaacttg atggatatct tctacaaact
    66841 gacacatgat ccttaacccg gcaatatcag acatttcttc ttctaaacgt cccattggaa
    66901 tatgccgtaa ttgtgatttt gttacaatac tatcgactgg cttgactctc ccagtgacaa
    66961 attcaatcgg gacatgtcga ttttgttcac ggaactgttt gcggatcccc cgcagtttca
    67021 ctttcaattc tgatacagtt tgttcatatg gtgctaaaaa tgcttcccaa tctcgttcca
    67081 tagctgatac ctctacttca ttaacattca ttgtttattc taccatatat gcaaagtttt
    67141 ttcgtactct tgatttatgc attgaccaaa aaaagaaaat taggcaaaat agacaaaaag
    67201 gagtcgatca aatgacacaa atgcttgaaa tcgaatataa atctatgctg acaaagcaag
    67261 agtacgagat attcatcgct cactatcaat taacagataa agatttccgt gtacagacta
    67321 atatttattt cgataccgtt gatgaacagc tgaagaaaca aaattgtggc ctacggatcc
    67381 gtttcgttgg tcatcaagct gaatatacat taaaaacacc tgctgaaaaa gggcgtctcg
    67441 aaacaacgga tacctttata acagaagaag cagaagcatt tatcttcgaa aaacgtctgc
    67501 ctcgttcagg cgcagttttt caaaaacttt ctgatttgaa tatcgatcct gcttcattaa
    67561 tccaaaccgg aaagcttaca acaaaacggg ctgaattccc tattgaagaa ggacttttag
    67621 ctattgatga aagctgggga gacaatctac acgattttga actggaacta gaggtaaggg
    67681 gatgcatctg tgggaaaaat tgcttttata aattttttaa aacgtttttc tttaccttat
    67741 cgtcctgcta agaacaagat ccaacgtatg cttgaagcta aaaattagaa acctttcatt
    67801 ttcacaaggc acttgagttc atttttttac ttttttctgt taagttatag gatgtgaaag
    67861 aatctagtca ctagttgtat agaatctatt ttgggggaga ttcatatgat cgaaatctat
    67921 ttatttgtta atcctttagg ggaatactgt ttcgaaatgg aacaacagtt attacaattt
    67981 atcgaagaag agtacgggac accatcaaaa gaaaagatgc aatttcgctt tcttcctttg
    68041 gtcaatttgc agacaattgg ggatgtcatg caacaaaaag gaatttctca gaacaatctt
    68101 gaggaacgta atcacttatt ctcaatgact tattctgcag cattggactg caaagcggcg
    68161 cagtttcaag gaaagaaaaa aggccgccgt tttttgatca aattgcaaga agcagtcggc
    68221 tgtaatgaca taccatattc tcaagaactt gctgaatcca tatttgaaga aatcggcggc
    68281 gatttgaata tgtttaaaga agatcgtcag tctgacttcg ttaaagaagt attcttttct
    68341 gatcaaaaaa ttgctcggga aatgggcatc acaaaacacc cttctgcagt cgtttacaat
    68401 tatgcctgcg accgtgattt tggtgtttta gtagaagtaa aaggtacaag tagcctttgg
    68461 agaatcacag agctgtgtaa aacaacagat aactcatatc aagtttttaa tggtgaacat
    68521 ttacatacaa atgattctca gttccattca agaactagcc atttacactt gctttctaat
    68581 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
    68641 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
    68701 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
    68761 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
    68821 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
    68881 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
    68941 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
    69001 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
    69061 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
    69121 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
    69181 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
    69241 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
    69301 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
    69361 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
    69421 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
    69481 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
    69541 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
    69601 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
    69661 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
    69721 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
    69781 tctaaattgt tcgtaaacac tgtacaatcc cttaaatgct gcttcgcgca cccttcgatc
    69841 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
    69901 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
    69961 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
    70021 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
    70081 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
    70141 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
    70201 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
    70261 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
    70321 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
    70381 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
    70441 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
    70501 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
    70561 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
    70621 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
    70681 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
    70741 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
    70801 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
    70861 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
    70921 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
    70981 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
    71041 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
    71101 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
    71161 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
    71221 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
    71281 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
    71341 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
    71401 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
    71461 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
    71521 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
    71581 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
    71641 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
    71701 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
    71761 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
    71821 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
    71881 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
    71941 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
    72001 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
    72061 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
    72121 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
    72181 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
    72241 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
    72301 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
    72361 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
    72421 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
    72481 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
    72541 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
    72601 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
    72661 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
    72721 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
    72781 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagt
//
LOCUS       DANKBF010000005        68094 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000005 DANKBF010000000
VERSION     DANKBF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(143..2329)
                     /locus_tag="PWY89_000364"
     CDS             complement(143..2329)
                     /locus_tag="PWY89_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2065425.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2547..3230
                     /locus_tag="PWY89_000365"
     CDS             2547..3230
                     /locus_tag="PWY89_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL2065426.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3311..4030)
                     /locus_tag="PWY89_000366"
     CDS             complement(3311..4030)
                     /locus_tag="PWY89_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2065427.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4342..4548)
                     /locus_tag="PWY89_000367"
     CDS             complement(4342..4548)
                     /locus_tag="PWY89_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065428.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4793..5395
                     /locus_tag="PWY89_000368"
     CDS             4793..5395
                     /locus_tag="PWY89_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2065429.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5536..6816
                     /locus_tag="PWY89_000369"
     CDS             5536..6816
                     /locus_tag="PWY89_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2065430.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6829..7557
                     /locus_tag="PWY89_000370"
     CDS             6829..7557
                     /locus_tag="PWY89_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2065431.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7626..9149)
                     /locus_tag="PWY89_000371"
     CDS             complement(7626..9149)
                     /locus_tag="PWY89_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2065432.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9401..10582
                     /locus_tag="PWY89_000372"
     CDS             9401..10582
                     /locus_tag="PWY89_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2065433.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10642..12270)
                     /gene="treC"
                     /locus_tag="PWY89_000373"
     CDS             complement(10642..12270)
                     /gene="treC"
                     /locus_tag="PWY89_000373"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL2065434.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12721..15432)
                     /locus_tag="PWY89_000374"
     CDS             complement(12721..15432)
                     /locus_tag="PWY89_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065435.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15442..16143)
                     /locus_tag="PWY89_000375"
     CDS             complement(15442..16143)
                     /locus_tag="PWY89_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065436.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16329..16928
                     /locus_tag="PWY89_000376"
     CDS             16329..16928
                     /locus_tag="PWY89_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2065437.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17009..18022
                     /locus_tag="PWY89_000377"
     CDS             17009..18022
                     /locus_tag="PWY89_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2065438.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18130..19047
                     /gene="rbsK"
                     /locus_tag="PWY89_000378"
     CDS             18130..19047
                     /gene="rbsK"
                     /locus_tag="PWY89_000378"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2065439.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19075..19470
                     /gene="rbsD"
                     /locus_tag="PWY89_000379"
     CDS             19075..19470
                     /gene="rbsD"
                     /locus_tag="PWY89_000379"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2065440.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19470..20843
                     /locus_tag="PWY89_000380"
     CDS             19470..20843
                     /locus_tag="PWY89_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2065441.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21200..23344
                     /locus_tag="PWY89_000381"
     CDS             21200..23344
                     /locus_tag="PWY89_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2065442.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23396..24436)
                     /locus_tag="PWY89_000382"
     CDS             complement(23396..24436)
                     /locus_tag="PWY89_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2065443.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24713..26515)
                     /locus_tag="PWY89_000383"
     CDS             complement(24713..26515)
                     /locus_tag="PWY89_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065444.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26529..27281)
                     /locus_tag="PWY89_000384"
     CDS             complement(26529..27281)
                     /locus_tag="PWY89_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065445.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27290..27652)
                     /locus_tag="PWY89_000385"
     CDS             complement(27290..27652)
                     /locus_tag="PWY89_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2065446.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27828..28700)
                     /locus_tag="PWY89_000386"
     CDS             complement(27828..28700)
                     /locus_tag="PWY89_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2065447.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28751..29239)
                     /locus_tag="PWY89_000387"
     CDS             complement(28751..29239)
                     /locus_tag="PWY89_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2065448.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29377..30168)
                     /locus_tag="PWY89_000388"
     CDS             complement(29377..30168)
                     /locus_tag="PWY89_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL2065449.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30223..30831)
                     /locus_tag="PWY89_000389"
     CDS             complement(30223..30831)
                     /locus_tag="PWY89_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL2065450.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30824..31141)
                     /locus_tag="PWY89_000390"
     CDS             complement(30824..31141)
                     /locus_tag="PWY89_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2065451.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31297..32025)
                     /locus_tag="PWY89_000391"
     CDS             complement(31297..32025)
                     /locus_tag="PWY89_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2065452.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32184..33065
                     /locus_tag="PWY89_000392"
     CDS             32184..33065
                     /locus_tag="PWY89_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2065453.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33302..34345)
                     /locus_tag="PWY89_000393"
     CDS             complement(33302..34345)
                     /locus_tag="PWY89_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2065454.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34348..35016)
                     /locus_tag="PWY89_000394"
     CDS             complement(34348..35016)
                     /locus_tag="PWY89_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2065455.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35158..37494
                     /locus_tag="PWY89_000395"
     CDS             35158..37494
                     /locus_tag="PWY89_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2065456.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37609..37920)
                     /locus_tag="PWY89_000396"
     CDS             complement(37609..37920)
                     /locus_tag="PWY89_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065457.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38101..39576
                     /locus_tag="PWY89_000397"
     CDS             38101..39576
                     /locus_tag="PWY89_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2065458.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39623..40207
                     /locus_tag="PWY89_000398"
     CDS             39623..40207
                     /locus_tag="PWY89_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2065459.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40209..40628
                     /locus_tag="PWY89_000399"
     CDS             40209..40628
                     /locus_tag="PWY89_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL2065460.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40909..41193)
                     /locus_tag="PWY89_000400"
     CDS             complement(40909..41193)
                     /locus_tag="PWY89_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL2065461.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41545..42255)
                     /locus_tag="PWY89_000401"
     CDS             complement(41545..42255)
                     /locus_tag="PWY89_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL2065462.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42252..43724)
                     /locus_tag="PWY89_000402"
     CDS             complement(42252..43724)
                     /locus_tag="PWY89_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2065463.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43862..44335
                     /locus_tag="PWY89_000403"
     CDS             43862..44335
                     /locus_tag="PWY89_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2065464.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44488..45039)
                     /locus_tag="PWY89_000404"
     CDS             complement(44488..45039)
                     /locus_tag="PWY89_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2065465.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45144..45674
                     /locus_tag="PWY89_000405"
     CDS             45144..45674
                     /locus_tag="PWY89_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL2065466.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46157..46600)
                     /locus_tag="PWY89_000406"
     CDS             complement(46157..46600)
                     /locus_tag="PWY89_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2065467.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46616..47776)
                     /locus_tag="PWY89_000407"
     CDS             complement(46616..47776)
                     /locus_tag="PWY89_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2065468.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48283..49983
                     /locus_tag="PWY89_000408"
     CDS             48283..49983
                     /locus_tag="PWY89_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065469.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50085..50918)
                     /locus_tag="PWY89_000409"
     CDS             complement(50085..50918)
                     /locus_tag="PWY89_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL2065470.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51222..53387)
                     /locus_tag="PWY89_000410"
     CDS             complement(51222..53387)
                     /locus_tag="PWY89_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2065471.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53647..55941
                     /locus_tag="PWY89_000411"
     CDS             53647..55941
                     /locus_tag="PWY89_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2065472.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55934..56599
                     /gene="pgmB"
                     /locus_tag="PWY89_000412"
     CDS             55934..56599
                     /gene="pgmB"
                     /locus_tag="PWY89_000412"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2065473.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56592..57599
                     /locus_tag="PWY89_000413"
     CDS             56592..57599
                     /locus_tag="PWY89_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2065474.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57621..58640
                     /locus_tag="PWY89_000414"
     CDS             57621..58640
                     /locus_tag="PWY89_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2065475.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58921..60288)
                     /gene="nhaC"
                     /locus_tag="PWY89_000415"
     CDS             complement(58921..60288)
                     /gene="nhaC"
                     /locus_tag="PWY89_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2065476.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60397..61818)
                     /locus_tag="PWY89_000416"
     CDS             complement(60397..61818)
                     /locus_tag="PWY89_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2065477.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62061..63938)
                     /gene="tdc"
                     /locus_tag="PWY89_000417"
     CDS             complement(62061..63938)
                     /gene="tdc"
                     /locus_tag="PWY89_000417"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2065478.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64229..65485)
                     /locus_tag="PWY89_000418"
     CDS             complement(64229..65485)
                     /locus_tag="PWY89_000418"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2065479.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65597..65802)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66065..67366
                     /locus_tag="PWY89_000419"
     CDS             66065..67366
                     /locus_tag="PWY89_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2065480.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67435..68034)
                     /locus_tag="PWY89_000420"
     CDS             complement(67435..68034)
                     /locus_tag="PWY89_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2065481.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20420 a        13811 c        12119 g        21744 t
ORIGIN      
        1 acctcccttt tccgaataaa ctgtgttcca gaaatagctt cttcgaaaat aagctgaaat
       61 ttcacaaaaa tttgagaagc aattttcgta cattccttct tctttcttga aactgaccac
      121 ttctgtcaca ccctctcttc tatcactttc cttttttcac acgactcccg gtttctttga
      181 taaatctcga tactttcaat tttcttgaat ttttcatttg aggtacacat agatacagac
      241 gatttcttgc tctagtcatc gcaacataaa atagtcttcg ttcttcttct atcgtcgtta
      301 ctggttctgc ttttatttca ttaaagtcga ttactttctt ttctgtcgct ccgttataag
      361 gcaaacttcc ttcgttacaa ccaatcaaaa agacatttcg gtaactaaga cctttacttt
      421 tgtgaatgct ctggatacat agtgcatcaa gttttccttg tctgtaagca atcaagcggg
      481 tttttaattc tttagctgtc gcttgggttt cttgcgataa ggcttggagt cttctgaaag
      541 tagcatgctg ctttacttct tctcgtatcc attcttgatc actctggtcg atttccttag
      601 ctatacgtgg cagatgaaga tagctttgaa caagcgaata gatttcttga tgctggtctg
      661 ttttacgcaa cgcttcctca aactctcgga tttctgcaga tttcgatttt aaattggggc
      721 ttatcgacaa aatataatct gcaacggcta tattttgata tttaggagaa aagtattgat
      781 cgcattcact ttttttcagg tagcagtctg ccatcagctg ccggagcaat tgctggtagg
      841 cctgattttt cttttgcaga ttcagtgctg tttgccaatg aacaattttt tgatagaaat
      901 gttggaaaga cttgatacca aagatacctt catatggact atctgcttcc agtacaaaag
      961 gaatattcgt tttcagcaga gattggacaa gctgctgcgt ttgtgaagct gatcggtata
     1021 atatgattga ttccttatat ttcccatttt tttgaataaa tgtctgaatt tgtttgatta
     1081 gccactgact ttctgcttta gtatcagaaa acaaataggc ttccataaca cctgcttctt
     1141 tttgtgctgg aatcatcgcc tttggtatcc gatcattttt attatagctg atcaatctat
     1201 ttgctccttg gacaatagct gcccgagaac gataattagt taataagaaa agagttttgg
     1261 taggatattc tttttcaaag ttcttgatgt atgctggctc tgatcctctg aatccataga
     1321 tactctgatc atcatctcca acaacaatca acttgatcct gttttccttt gtcatccgtt
     1381 tcacgatttc ccattgaagt ggattagtat cttgaaattc atcaatgaat agataatcat
     1441 atttttggcc gatataatgc tggatctcct cattctccaa agcgcttttc agattttgga
     1501 gtacatcatt gaactctagc aattgctggc ttcttttttg ttcttggagt tccgtgagca
     1561 atccatacag tcgttctgat atcggctgtc gtgcattctt atcaatggaa tcatattttt
     1621 ctttaactaa ctgaaccatt tcttctactg aaaggttcat attctgccaa tagtctaacc
     1681 gagaagcaat cgttttttct ccatattgat caaatatata aagattatat ttacgtatca
     1741 gctgttgcaa aacagcattc actttttgtg cagtatcctt tgaaaagtct ccttgtattc
     1801 cttgcattgc tacttcagga aattttcgac aaatttcatt gtatatccta aaaaagatac
     1861 tatggaatgt tccgatatgg acagcggttt ttctctggct gaaattatct actcgttttt
     1921 ttagttcatt tgcagcgttc cttgtaaaag tgatcattag tatgcgttct tcgtctactt
     1981 gtttttgcgt catcagataa acagcttttg cacagattgt ttgcgttttg ccgctcccag
     2041 ctcctgctat gattcgaaga tgtttcttca tatcatatgt gatagctgct ttttgttctt
     2101 gattgaagcg attataggaa cgtttctcca tcattttaaa gaaagagctg tttccttcac
     2161 tttcttttac ttgtacttct tcctcaataa gtccgggtgc ttcgccataa gccgcttcat
     2221 aggtagcaat gatttcttct tgtagccgaa agacttgttg aagcttttta tacttgttct
     2281 cccagcgttt caattctttt tgtaatcgga gaatttcttt ttcattcaac tgattgattt
     2341 ccttcccatc tttcttcttt gaatttgttt atttcaagtt ggttatttaa agtttcgttg
     2401 tctatttttt ctttatacta acagaccttt ctaaaaatga aaatgggaag cgatatgatt
     2461 ttagttttct gaaaattttt cctttttttc aatcgtttat gctaagataa ggaagacaaa
     2521 aatattcata aaaggtgatg aaaaaaatgc agacttcctt tcacgagggg cgtacaatag
     2581 gacttttgct gacatttatc ggaggagcca tggacagtta tacttatatc caatatggtg
     2641 cttttgcctc tgctcaaact ggcaatatca ttctatcgat catccaagca tttgacggag
     2701 aatggctaag tgttggtaaa aaagtattgt ctaccctttt ctttttctta gggattcttc
     2761 taacaaagta tctaattgat tattttagaa aaaaagaaaa acatttctgg cgcttgtttg
     2821 ttctatatta tgaagcagtg atttttttct ttgttagttt gacacccgtc catgagcatc
     2881 ccgcattagt aacgattttg attgctttta ctgcatcgat ccagtgggta gcttttgata
     2941 aaatcaacgg gcgtgcctac actaatttat ttacaactgg aaatttaaaa ggggtagcaa
     3001 cgaacttata tgattattgt atttctaaag aaaaagcaga ttttgatcga tttttccact
     3061 atctaagtgt cgttcttgcc tttatcgcag gagctattgt ccttgttttc tgtcatcatc
     3121 tgtttggcgc ccattcgatt ttgatcgttt ccattttatt tttgatcatg gccctcactc
     3181 agacatttca tttatggcaa ttttatcata cgaatcggtt cactatttga tctcaatgaa
     3241 tcaaaaaaac taagcaagat gctgctcaca gagaaatatc tgcgagtgag caatttgctt
     3301 agtttttata ttatttagtt gtaaagattc cctttgcttt cgggttattt gtagtgtagc
     3361 ggatcgactg ggcatctgcc tcttctgtca ttttctttgc ccgcatcttg atttcttgta
     3421 cgatttgttc tccacatgcc ggatcggcta cgaaatcata tttgtcccca tcgatcacta
     3481 ttttgggaca tatatcaaac tgttcatacc actcttcgta tcgtcgattg agttctttat
     3541 agtaggaata gagttcagga tcttgttcta gctgttcata ttcccggcct ctttttttga
     3601 ttctcgctaa catagtttca aaggatacac gaatataaac cagtagatct ggtttctttt
     3661 gtggcgaaat ttcttctaac tccttcagca tcgtttctaa aagaccttca tactgcttta
     3721 cttctacttc agaaacacgc cctaaatctg cattcaaatg aaacagcata ctatcttcgt
     3781 agatggagcg gtctagtacg ttgtcatcat ggctgaaagc atctttgatt gccaaaaagc
     3841 gtttgttcaa aaagaaaatc tgtaataaaa aagtatactt ttctggatta gaataaaata
     3901 gcggcaatac ttcattatct tccacgttct cataaaaagg ttttgtttct aaatcttggg
     3961 caagcatttc agttagtgtg cttttccctg caccgatcgt tcctgcaaca atgatcacaa
     4021 ttatcggcat ccttcctgta ataaaatctg ttcttcattt tactacttca tttatagaaa
     4081 taaaagcctt gactttatct gctccttaaa agctacttct ctaaaagtag ccaaaagtaa
     4141 ctcctattca tgaaaataaa ggctgtgaca aaagtcttgt cgcagccctt ttttcaaata
     4201 aacagtattc tcctgccaac acctcggtca caaggcaata ttcataaaac atgaaaagct
     4261 gttttttaac attattcctt gtaactcaga gctaaacggg agactcacaa cctctttatg
     4321 ctctcaacat tttatctgtt tttattcttc tattgtactt gatttgcgta caactaactg
     4381 ccagtgcaga ttatgagcgg tatcattgtc ttctactgcg catttcatga cacgtatcat
     4441 cacactattt tcatagattt tttctatttt tccttttatt ggtgattcaa ataaatcaga
     4501 tttaaatgca tatgtttcgc cgacttcaaa caattgttca ttttccataa gaatccctcc
     4561 atcacctttt ataaggtcag taatttttta acataaatca ctcgaaaaag cacaaacttg
     4621 tcataaaaag tacttttcgt atcaaaactg tcactttttt tggtaaggtt cttggaaaac
     4681 gaagattttt ttaggagatc atgacggttt tgatgtcaaa accacttttt atgaaacaaa
     4741 agacgttttt tatttttttt agcataaaat ttgtggtgga ggtacgtatg agatgaaaga
     4801 taaagttgtg aacaaattat tagaagcctt attcagcgga gacaaatcgg aagaagatat
     4861 ggtgtatgtc caagtaaaat ttgtcttaga agttttattg aataatcttg gaaaactagc
     4921 ggtcgttcta gtcttttctc tggtgacagg ctcatgggcg gaaacaggaa tcacctttct
     4981 ttcttatatc tgcatccgca gatatgctta tggattgcat tccgactctg aattcgtttg
     5041 tctgctgtgg acactacttt atttatgggg cgttcccttg gtgatgaaac atctgcaatt
     5101 gactatctcc ttcccaatga tggtcttttt gttaatcagc tgctttttgt tactgcttcg
     5161 ctatggatca agaggtacag caatcaatcc cattgaaccc gagaaacgtc ccccattatt
     5221 aaagaaggcg atcagcatgt ttctaatttt ttctctcatt acgttatttt tctcagcttc
     5281 ttacttttct acctatttgc tacttgggat tgttttagaa atagctacgt tgcttcctat
     5341 tacaaactac ttgatgaatt ttggaggaat ctttcatgaa aaaaacaatc attaaacaat
     5401 taactaactt atctgacttt tttgtatctg gctgctgctt tggtatgagt tctgaattca
     5461 aaatccctga aaaattaaaa aaataatacg ccaattgtgc tataatttgg taaaaatcaa
     5521 tgggagctgt actttatgtt tttatggtcc atagcgttgc aatatttgat ttacacaatt
     5581 tgtatcttat tgattgacca tcacatccga tacaaatatc tgtttgcggg agtcattctg
     5641 atttcttgta ttttgattgg atgggtctat tcgattttag ggatttttac tgctctaatt
     5701 gctataggaa tgctgatgct tacttttttc ttcttttcga cttcgcgtca acaattggtt
     5761 tacgcgcttc cttttgggtt attgacgtcg cttttaggag atcacttaac aagcatcggc
     5821 gatttttttg ttttgaaatc ttcggttatt gaaccaggag attcattgca gtattttcac
     5881 attgtcttat ccgctttttt atctgcttta ttagcttttt gtttaaaaaa atcgttagag
     5941 acttggtttc acactgtgga ccgccgtatg atcggtacga tcgggacatt gatattattt
     6001 acatactata tcattatttt ttatacccgt ttttctggtg aaacacctga tgtcttagct
     6061 ttgaatacga gttttttcat tctatatctg tgtgttggcc tgatcatctt ggtttattat
     6121 tggaaaatct ctaataagaa attagctgat cagttattag agcaacaagt caggatgcaa
     6181 caggattata tcaaagatct agaaaaaaac tatcaagact tgagagaatt caaacatgat
     6241 taccaaaatc tgcttttttc tcttcacagc tatatttcag aaggcgactt agaaggatta
     6301 cggtcttatt atgatcaaac gatattacct acacgccaga tgatgaacct ttttccggcc
     6361 aatctaagta tgttggataa tatgaaagtt cctgagatcc gtagtttact gtcattaaaa
     6421 ctcatgatgg cacaggaaaa agggttgatc gtccagcttc attttcctga gccaatcgaa
     6481 ctggatacga aacatacggt aaaccttgta cgtatgcttg gtattatctt ggacaatgcg
     6541 attgaaggag ctagtgctgc aaagaagaaa aaaatcgaat tgaccatact caagaagaat
     6601 cattccgtga tgattcgtgt gatcaataca acaacaaata cattaccgtt aaaccagttg
     6661 aaacaaaaag ggttctcaac aaaaacaaat ccaaaaaacg aaggccttgg cttgtttatc
     6721 ttggatgaat tgacaaagac caatccttat cttttccttg aaacatcaat tgaaaaccag
     6781 cgtttttctc aaacgattta tatacaaata aaataaagga gtaatgatat gattcccatt
     6841 tatatttgtg aagacgatcc aaaacaatta gaagttttat caacggtgat caaaaataga
     6901 atcatgattg aaaacttaga cagttatgtc catatttcta cctctgatcc ggaagaactt
     6961 ctcaatgcag ccaagatcag aacttcttct tttggtattt actttttaga tatcgaacta
     7021 gaagacagtg aaatggatgg tgtagcgatc ggtaaattga tccgtgaatt agatccttta
     7081 ggaaaaataa tctatgtgac ttcccatacg gaaatgtcga tgaaaatctt aaaaagcaac
     7141 atcgaaccga ctgattatat cgtcaaagaa gatctttatg atttaaaaga aaatgtcgag
     7201 catatcctga gcaaaatttt cgaagatttt caaatgtccc aaatccaaaa agatatcttc
     7261 aaaatagagt ttaatgatga gatcaaattt ttaccgatca aagacatcca atatttttct
     7321 acttcgctag gagcgccaca taaactggaa gtccatttga ctcatgccca attacaattc
     7381 tatgagaaga tcaaagaaat cgagaaaatg catcggaatt ttatccgttg tcataaatcc
     7441 tatgttatca atacacaaaa tgtgcggtcg attaataaaa aaaccagaga agtcacgttg
     7501 gcaaacggcg agaccatccc agcctctatc cgtggattaa agaaattgat ttcataaaaa
     7561 gaaccgaata gtttggaccg actaaatgac cagccgatcc aaacccttcg gttctttttt
     7621 gcctatcagt gcaaaagcat ttcctgtgca atttcacgtc catccattgt atctggataa
     7681 aatgttggcc aattctccat ttctttcagc aatacttctt gactttcatt tcctgcatat
     7741 aaatgaaaat gttcggagtc agccggtgca atcgtgtgat cactgaattg aatatatcta
     7801 aaagcgccac tttccttatc ttctgcttcg aatagatacc ttacgccacg gtttcctgcc
     7861 tcgtaggtca agatttcttt ccctacataa cgataatttg aacgtttcgt tgttccattc
     7921 ttaaacgtaa atgccatctc tttctcactg atcttgattg aagcaatatc tgtttgatag
     7981 cccttcgtat aatattcttt atattcttgt gctgtcttat cctgattcaa tttttcttta
     8041 tattcaaaaa caggatcaaa agtcccatct tctacatacg tatctactgt ttgccaagtt
     8101 cctgcccaat ctgaaagaga gcgatcttgt acatcctcgt cttcaaaata accgttgtag
     8161 acgctctttt cttttttggg ggtcaaagta tcttctagcg ggttttctgt ctctgtcgtt
     8221 tttttcaacg cttcgaggtt tgcttccatg atagaaagat agttttctcc attttctatt
     8281 tgttcattcg tcaatccttc caaaggattc aatacttcta aagatacacc tgcttcgttt
     8341 gctaacgttc tggcaatact atctttggcg ttttcttcaa aataaatata gttgattcca
     8401 tgttctttaa caaagtgttt cagttcagca attctcgccg cagacggttc ttcactagaa
     8461 gaaagaccag caatcgatac ttgattcaat ccatattcta acgctagata gttgaatgca
     8521 gcatgttgcg tgacaaaaga tctttgtttt gcttgcttaa aggtttcttg ataaagctga
     8581 tggagtgctt gaagtttctc ggtataggct gctgcatttt ttgtccatac ttcctgtttt
     8641 tctgggtaag cttcaatcag ctgatcacgt atgtttgcta cctgcttgat tgctaaactt
     8701 ggagctagcc atacatgtgg gtctagctca tgtgtatggc cttcttcccc gtgttcatgg
     8761 tcatgatcat gtccttcttc acttcccgga agtaaaagca taccttctgt cgcttttatc
     8821 gtatggacat tgccttcttg taatgttttt tggattgcgg ggacccatgt ttccatgttt
     8881 tcatcattat aaacaaagat atctgtatct tggatctttg ccatgtcttt agcagaaggt
     8941 tcataatcgt gggaatcagt ccctgctgga atcaataact ctacttctcc ttcatccccg
     9001 acgattgctt ttgtaaaatc atacatggga taaaaggtcg tcatcacgct caatttttct
     9061 ttactgtcct cggctttccc acttgccccg caagcagtta acatacctgc taaaaccagc
     9121 atggccaata tacctatcaa tcgtttcatt tatgacactc ctcttctcat cttctttcat
     9181 ttgaatgttc cgattcgctt ttcgcaaacc gtaatagtta cgttttagca acaggattta
     9241 atttatcata tggttaaaag aactgtcaag cgttttctta ttttaattga tgagaaagtt
     9301 tatatgaatg attgacgata tttttgcaat caacctctat cattttcctt ttatacccgc
     9361 ttttgtctat aatgaattta tagataaagg aggaaaaaga gtgaaaaaag aaacagatac
     9421 aacgaatcgt ttgatccgct ttttaggagg aaaaacctct tactatgttt taggaatagt
     9481 tatcttactt gcgctgatca tttatttttt ccatcagatc tcgtttatat tcaagcctgt
     9541 cggaatcatt atcgctacta tcttgccccc attattgttt gcactgatct tgtattatct
     9601 tttggaacca gtcatcaaac gtttgagcaa gcacctttcg aggaacttgt cagtcattat
     9661 cgtttatgtg attattttat ttttattggg gcttggaagc atatggctta ttccttttct
     9721 tgaagaacag gcaaagacat tgatcagtag tttgccagat ttgtttcaag attttcaaga
     9781 gagtctccga caatttttgg agaaaacacc ttttgccgat tcatttaatc aatttttcgc
     9841 ttctattgac gacgtgacgt ctgatatagc tggatttatt ggaaattatt gggaaagtgg
     9901 cgcacaacgg atcagcaaca ttttttcaac tgttacagct atttttatta ctttatttac
     9961 tggtccaatt gtcgcttttt tccttttgag agatcctcaa aaattttatc agtcagtttt
    10021 agcgattgtc ccaccagcat ttcgtacaga ttttaaaaat ttgacaaaga ttgctaatca
    10081 acaattgggt tcatttctaa aggggcaaat cattgcttca tttatccttg gagcagtcta
    10141 ctgggtttgt ttcttactga tcggattaga atttgctagt gttttagcta tttctgctgg
    10201 gttgttatgt atcattccat atattgggcc gtttatcgcc ttctttccag gtttgatcat
    10261 tgcattccaa gactctactt ttatggctgt caaatttgtc attgtctggt ttgccgtaca
    10321 acttctccac ggtgacttgg tgattcctcg agtaatgggt gatcgtttac agatccatcc
    10381 gattacgatc ttaatcgttt tacttgtcat gggtgatcta atgggaattg tgggcgttat
    10441 ttttggtatt cctatctata cgttagtcaa attactcgtt acttttattt tccgtaaatt
    10501 caaacaacgc tacaataaat tttatggcga taaaggagag tatgaaaact cttcattttc
    10561 tgaagaagat tattttaaat aaaacacaaa acgtcgatct tacgagaata gctcgtagga
    10621 acgacgtttt gtgtttttgt tttaattttt cagcaaagca attgcctggt atggttttag
    10681 ctccagactt tgatgaagaa cagtatcgga ataattatcg ataagaatat tggcttctgt
    10741 gaatccttct gggatggcga tccttgtatc tttatcatag aaatttgtta atacaagcag
    10801 ctgctgtcct tcaaattggc gaagaaagcc ataaacttcc ggatgatttg cttcataagc
    10861 atgataatct ccttgtgcaa cgattggata tgtttttctc agccgaatca atttcttgta
    10921 ataggacaaa atagaatctt cagaagatag ttctttttct acattaatct cttctagctt
    10981 gcctccaact tttaaccaag gtttccctgt agtaaatcct gcttgttctg tgtctgacca
    11041 ttgcatgggt gttcgagagt tatcgcgaga ttttgctttg atccgacgaa acgcttcttt
    11101 ttctgtgaat ccttgttcaa ggagtaactt ataagcattc aaactttcta cgtcaatata
    11161 gtcggaaatc gatgaatagt ctgggtcgat catgccgatt tcttctccca tataaatata
    11221 gggcgtccca cggttcaaat ggatcatggt tgctagcatc tctgctcctt gttttcgata
    11281 ctgatcgata gctacaaatc gattgagcgc acgtggctga tcatgattat taaaaaatag
    11341 agcattccag ccgttaccct cactcatctt ttctccccat gtatgaaata gtcgctttaa
    11401 ttctgcaaaa tcaaatggag gagtagacca tttattccca ttttcataat cgactttcaa
    11461 atgatggaaa ttaaagacca ttgataattc ttctctacta ggttctgtat acaagataca
    11521 attttcgatc gtcgtagagc tcatttcacc gactgtgatg atagtggaat ccgtcccaaa
    11581 cgttgcttgg ttcaattctt ttagatacgt atgggtgatt ggtttgtccg tatattcagg
    11641 tttgccatca ttttctttat tatttttcaa ttgttcgtct ttaccgataa cattgataac
    11701 atcgaaacgg aatcctttta cccctttttc catccaaaaa cgaacaactt tgaatagttc
    11761 ctctcttact gcttggttcc gccagttcaa gtcagcttgt gtccgatcat ataagtgcaa
    11821 ataatatttc cccgtatctc caaatggcga ccaagcactt ccaccaaatt ttgataccca
    11881 atccgttggc tcttctctta aaatgaagaa gtcctgataa tgctggtccc ctgctagtgc
    11941 ttttttgaac cattcatgtt ctgtcgaaac gtgattcagc accatatcga gcatgatttc
    12001 tatccggtat ttctttgctt cggctattag ctgttcgaat tcctccatcg taccaaagat
    12061 aggatcgatg gctgtgtaat cagagatatc ataaccgttg tctttttgag ggcttggata
    12121 aatagggttt agccaaatca tgtcgatgcc tagatcattc agatacggga gtttttcttt
    12181 gattcctttt aaatctccaa ttccatcacc attactgtct ttgtaagatt tgggatagat
    12241 ttggtaaatg actttgtctt taaatgtcat ggatatttac ctccattcaa ctgatttatt
    12301 acctaccaca acttatatat acaagttatc atttattatt ataactgaac cgaaagcgtt
    12361 tgtaaaattt atctttatac attctaagag gtctgttgcc caataaaaaa caagagatca
    12421 gacatgtatt tgtctatcct cttgtttaaa atagcaaaat attcttgttt cttctataaa
    12481 tggtgttcaa aagcttttat ctacggcaat cagtcttctc aaatgtaaaa catgaggagc
    12541 taattttatt tgagtattct ttttgctcga tgcaggacgg cttttttaca acctctgatt
    12601 catggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa tatgaaaaac
    12661 tattttgccg tttgtttctc taatactcag gtcaaaccgc tgatttcaca acctctgttt
    12721 ttattcattc gatttcaacg catcaatcat atcgatatgc tttaatttga aatgggtaac
    12781 aatcatcaca ataaccgtaa atacgatagt taatcctgct gataagacat aagcccacca
    12841 tgtgatcact aatggaaaga tgacattctc catagacgct tgttgcaaga tgaagtcagt
    12901 caatatatat cctacaccaa atcctccgat gattcctaaa agagtaaaga tgatattttc
    12961 tcgaacaatg tacatcgtga cttctttatc aaaaaatcca aggactttta ttgtagataa
    13021 ttcgcgtaca cgctcggaaa tattgatatt agttagatta tacaagacga taaaagccag
    13081 cagtcctgat aatacgacaa agattaagac aaccgagtct aagttcgcca ttgattcttc
    13141 ttgcgtttcg atttgtttgg acatgaaaga cgtatttgtc acagcgtctg tggcaagcag
    13201 tttctcggaa agcatttctt cttcttttgt tgtcatctta tctgtacgca gcaaaaatgc
    13261 attatccatc atcgattgat cagtaactga agcataatat tcaggggaca tataaataaa
    13321 gctacctaga tagtttttca cgatttcttt tacttcgact ttccattctt tttgatcttc
    13381 atcataaagc gaaatatgat ctcctgtaga taagtcaaat agttctgcta ctttttgcgt
    13441 aatagcgatt cctgttttag gcaaggagcg tgggttctct tcaagatcag ttaaatggat
    13501 aaaacgatcc aaatcagctg ttttacttgg tacgatcact gtcagacttt gcgaagcatg
    13561 tccttttttt ctcaattcta cggtttgagt atgaatagct aggatctcat ttactttttc
    13621 ttgcttctgc agcatatccg ctgctttttg acgactatct tccgtggatg aatccaatgt
    13681 aacgattgct tgatagtcaa tgatcggtcc aaattgtttg gctgaaacag aacttagaga
    13741 atctttcaat cccacgcctg caaccatcag cccggcacat ccagcaattc cgataatcgc
    13801 cataagcatt cttgacttat atcggaagag atttcggtaa ctgatttttt gattaaagct
    13861 aaggcgtgac cacagcggag tgatgtattc aagaaaaatg cgtttccctg gcttcggtgc
    13921 tttcggttga agcaatgcag caggccgttc tcttaaatct ttgaataaaa cgatcattgc
    13981 agcgccaaat gcggcaatga agaatgcggc agctgcttgt aaaatcggcg gccagtcata
    14041 aaaaataagc gcactcccaa tatcataccg ctcgttagac agctcaaaaa tgatccttgg
    14101 caaaagattc gttcctaata tcgttcctaa aacaatacct atccctgagg ctagacttgc
    14161 atagattgca tatttttttg cgatttcaac tttcgtataa cctaacgcct ttaatgtccc
    14221 gatttccctg cggttttctt ctaccatacg tgtcattgtc gtaaaggtga tcaatgcggc
    14281 aataaagaaa aagaaaacag gaaaaacatt ggcaatcgca gcgatacgat cagacagatc
    14341 accaaattct tgaaaaccga cattttctga acgttcattg tataggtatg atggtttagt
    14401 taaattttca attttttctg catttcgatt caattcttcc tctgcagagg ccagttcacg
    14461 ttctttctca actaattgcc tttcttgttc agcaagttga gcttcttgtt gagataatgt
    14521 ttgaacagcg attgttcgtt gcgcttcagg aagctgctgg atagttgctg actgttgcgc
    14581 taactgtttt ttagcatctt ctaaccgtgt tcttgcttgc gttgtttgtg ctttgccatc
    14641 cgctattttt tgctgtgccg gttctaattc ttcgttggcc gattttttca gctcttctaa
    14701 gcgttcttcg ggtcgatctt gtaaatactt ttctgccttt tcttgatttt gctccatttt
    14761 atctttgtaa gctttgctgt acgtattcaa attctttacg tttgtaaaat caagatataa
    14821 gacagaataa atatctgttt taaaaacgga ttcaggtaaa tagacaaaat aatcgacgct
    14881 accatttccg acgttcgcgt atcctctctc caaattgttg atgaacaatg gagaacggac
    14941 aaagcccgtg atggtatact ctttttcttt caattcatct gaatcaatct gataaatatc
    15001 accgatttta tacccttcat cttccgcttt ttcatccaat acaagctggt ttggtttttc
    15061 cgggagtttt ccatcagtta attgtagctt gttctgcttg ttatttttgt tataagaaaa
    15121 tatttgaatc acttcgtttt tgccagtatt ggaataatag atctgttttc ctgtttctac
    15181 ttttgcatct gggatctttt ctgcttgagc aatatctttt tgtgtcaagc caccagtaga
    15241 aacgatttgc aaatcagaaa gtcctgctgt accaacatag tgatccatcg ttttttgcat
    15301 gacaggtcca gcagttttga cacctacaaa taaaagtgtt cctagcatga caataaggat
    15361 gattgcaatg aatcgtcctt tagattgccg aatttcgcga aaacctgctt taagatatgt
    15421 tcttgttttc ataaaatccc cctaccatac aatctcatca atggatgaag gatgatcatt
    15481 taattcaaca gaccttactt tagcatcatt aatgtgaatg actctatcag cgatgggcgc
    15541 taaagcagaa ttatgtgtaa tgatcaaaac ggtattgccg tgttgtctgc tagcgttttg
    15601 caataatttc aatacttgct tgcccgtttc aaaatccagc gcgcctgtcg gttcatcaca
    15661 taaaagaagt ttgggatttt tagctagtgc acgagcaatt gaaaccctct gctgttcgcc
    15721 tcctgataat tgggacggaa agttattcaa tcgatgagct aggcccactt gcctcaatac
    15781 ctctacagga tcaagcgcat caggagaaac ttccgttgcc aattccacat tttcttttgc
    15841 tgttagatta ggaacgagat tgtagaattg gaaaacgaac cctacatctg ttcgtcggta
    15901 agcagtaagc tgtttatctg aaaattgcgc aatatctgtg tcatcaataa tgatctgacc
    15961 ttcatcaggt gtatccatcc cacctaaaat attaagaatc gttgactttc ctgctccact
    16021 tggaccaagt ataatcacta attcgccttt ttcaattgaa aaagaaatat cgtcatttgc
    16081 tgtaatggtc gtttctccca ttttgtatcg tttgtattca ttttttactt cgacatactt
    16141 cactctgatt cctccatttt cacgatattt ctcgctatat ctatgcatcg aatgcgtttt
    16201 ctcacactta tttattttac cactgaattt ctctagacta aagaaaggaa tcctttaatt
    16261 tagttgacaa ataaacgatt tatgatttac ttacataaaa aacattgtta tttgaaagaa
    16321 ggaacatcat gaatgattta aaggaaaata tgatttttga tttatcaaaa actgcttttg
    16381 tcgcgatcga catgcaaaaa gagatcgtaa ataacggtac actctctcca cacactgcag
    16441 catctattct gacaaacaat gatttgttag taagaacatt gaaacgtacc gctgccttga
    16501 aagttttagt gaacgtagac atctccactt tcccttattt gagtcaacaa actgatatgg
    16561 gtggaatgac tgcttctgta cctccagaat tcactgattt acttttgaag gattctttga
    16621 aagatacaga taatatgttg actatcacaa aatacaatcc aagtgctttt tttggtactt
    16681 ctcttgatct tcaacttcgc cggcgtggaa tcgaaactat cattttaagc ggtgtagcga
    16741 caacaaacgg tgtatatgct actgcattag atgcctttca acacgggtac catatcgtgc
    16801 ttgctgaaga tgcttgcagt gatcgtgaca aagaatccca tcaattgttc ataaaaaaga
    16861 tatttccgaa aactgcacga gtacgctcaa cgaaacaaat aatcgaagct atccaacaaa
    16921 gcaaataagt actggcacac gtgattttca tttacttctt tttgatttca tgggaaaata
    16981 accacaggat ttagatagga gtatgaacat ggtgaaaaaa aagatcacaa tcaaagaagt
    17041 cgctaaactt tccggcgttt caatcacaac tgtctccttg atattaaatg gaaatacgga
    17101 gaaattcagt ccgaaaactg ttcagaaagt tttagctgcc aaagaagaat taggctatca
    17161 gcctgactat tttgctagac aaatgattac caaggaaacg aaaacaattg gtgtactcgt
    17221 tcctgatatc acgaatccat tcttcagtac actgatgcga ggaattgagg atatcttgta
    17281 taagcagaat ttcgtcacga tattatgtaa tgctgattct gatcatcaaa aagaaatcga
    17341 atatctagcc gagctcacac gtcgaggtgt ggatggattc attatcgcga cttctgctgt
    17401 ctctacagat gcaatcaacg aaaatttgaa aaaacaaggc cggcccttca ttgttttgga
    17461 tcagaaaaaa tctgaaggat ttagtgatgc cgtacgaaca gacgattttc gcggtggtta
    17521 cctagcgggc atgcacttgc ttagtctagg acatcaaacg atcgcccttg tctatcctga
    17581 aaatccaccg gaaaatgtcc acgctcggat tgaggggttt aaatctgctc ttgatgttta
    17641 ccaaatccct cacgaccaac tgatcctctt acctacacag ttctcaaaac aaggtggcta
    17701 ccagatcaca gcagaacttc ttgattctgc tgcaactggt gtgtttgctt taaatgatga
    17761 attagctttt ggattatatc gcggattaga ggaagctgga aaaagcatcc ctgaagacta
    17821 cagcattatt ggctatgata atatagatat gtgcgagtat atcaagccga aactgacaac
    17881 gatcgcacaa ccaatcttcg aattgggaca aacatctgcc aagttattgt tagatcggat
    17941 ccaattccca gaaaaagaat gggaagaaaa aagattacct gtacgattag aaaaaagatt
    18001 ttcaactgca cccttgaaat agctttcaag gtgtgctata tttttatcga actattaaaa
    18061 cgttttacta aaacgtttta aaaaataatt ttcccatcaa aataaatgag atagttggag
    18121 gtttgtaaaa tgaacacggt tacagtaatt ggaagtatta atatggacac tacccttcgt
    18181 ttaaccaaca tgcccaagcc aggcgaaacg atgcatgcgc atgagatttt tcacgctggc
    18241 ggaggtaaag gtgccaacca agcagtggca gccaaacgtt caggcgcacg gacatctttt
    18301 attggaggcg ttggggctga cagcgaagga caacagttat tagatctact gacaaaagaa
    18361 aacatcgata catctgggat agctgaaatc caaggcgcca caaccggaca agcaatgatt
    18421 atggtagacg cagcaggaga aaatagtatc ttgatccatg caggggcaaa taatgctttc
    18481 catgaacaag aagtcctaaa aaacaagcaa ctgattacaa acagtgactt tattattgct
    18541 cagtttgaaa gtagcttaga tgcgacgatc ctagcttttt ctattgcaaa agacgcagga
    18601 aaaacaacga tcctgaatcc tgcaccagct cgtgaaacga ttcctactga actacttgaa
    18661 aagacagata tcattatacc aaatgaaaca gaaacagaaa tcattacagg aatcagagtg
    18721 acagatcata acagtttggt cgcagcagcc gaaaaactcc atgaattagg tattggaaca
    18781 gtaatcatta cactaggcag tgccggcgct ttctatcata cagaaaaaga acacggtatc
    18841 gtccctgctt ttaaagtgga tgccgttgac acaacagccg caggtgatac atttattggc
    18901 gcattaagca gcacacttca gccagacctt agtaacttga aagaagcgat cctttatgga
    18961 aatctagcct cttctgttgc tgtgcagtct tatggtgcgc aaccttctat cccttaccgc
    19021 gaagctttag aacaatcagc agtctaaaat taacaatgag aggaagaaac aaacatgaaa
    19081 aaaggaaaag taatcaattc agatatttca cgagtaatcg cacaaatggg gcactttgat
    19141 aaattaagta tcggggatgc aggcatgcct gtcccctttg gtactgaaaa aatagatttg
    19201 gctgtctcaa acggtcttcc aagtttttta tctgttttag aaaacgtttt agaagaactt
    19261 gaggtacaat cgattgaatt ggcggaagaa ataaagacaa tgaacccgga aatattggaa
    19321 aaaatcaaat tattgttacc agatacaccg attagcttta tcccacatga agaaatgaaa
    19381 aaagagttga ataactgtca tgcatttatt cgtacaggcg aaatgacacc ttacgccaat
    19441 attatattag tgagtggagt ggtcttctaa tggaaacgac aatgaatcct gaacagatgg
    19501 tactgtccga gcagattcat atgctgattg aacagttggg aggaaaagac aatattgatt
    19561 ctgtccttcc atgtgcgact cggcttcgta tcaaattgaa aaaacagcca gtagccaatg
    19621 cagaaaaatt aaaacagcaa atcccttcca ttaagtattt cattttactc gatagatatg
    19681 cagaaataat catggatcag ggattggctc ctgcttacaa ggaattgacg cgtcaaatcg
    19741 atcattcagc tccgaaagaa aagctaaatc cgatcgaaaa actatttggt ttgatatcgg
    19801 aaatctttac gccgatcatt ccgctgtttg ccggttctgg tatattaaaa ggtctggttg
    19861 ttttagcgac tacgattggc tggcttagtg aaacaagcgg aacttatcat attttgtctg
    19921 ctgcgtcgaa tgccgtcttt tattttctgc caatctttct tgctttttct gctgccaaaa
    19981 cgtttaaagt gaatggatat attgcggctg tcattgctgc agcactgatt gaaccgaatc
    20041 tgacctcttt actttcagac agtggcaaag cagctactga ttttctgggg attcctgtag
    20101 tactgatgca gtatacgtct actgtcatgc cggcgatttt aggtattttc ttttattctt
    20161 ttgttgaaaa attcctcaaa aaatatattc gcgaaaatat gcaattagta tttgtacctt
    20221 tactttcatt agttatcacg gttcctttga cgttaatggt attcgggcct gcgggtgttt
    20281 accttggcga aggtttagct gccgcaatta cttggatgat ggatattaac ggaccgatca
    20341 gtggcgcaat catcgctggt ggttggaata ttttggtaat atttggtatc caatgggctg
    20401 taaaccctgt catgatcagt aatatctctg cttatgggtt cgaccgaatc gttccgttga
    20461 caggagcagc caattttgga atggctggtg cagctttagg tgtatttctg cgaagtaaac
    20521 gttcaaaaac ccgttcgatc tccggttctg cttttgcttc gattttactt gcgggagtga
    20581 ccgaaccaac tgtttacgga attgcgatcc cattgaaaaa accatttgtt gctgcttgca
    20641 ttggtgcagc tgctggtgga gctgtaatgg gatttgccca agtgaaagcg atcgcttttg
    20701 tatttggtag tctgaccaca ttgcctgcat tcatttccgg tacttttttc tggtacttag
    20761 caggactggc tgttagtcta gtcgtagcaa tgatcactac tctcgttagc gggtttgatg
    20821 aagacctaat gtcttatgaa tagttaatga aaaagactga agtatgagat gtttatcaca
    20881 tatttcagtc tttttctttt taggatcatt atttttataa atttttgtga tactttccaa
    20941 actttggtat aatttacata tgcataacca tttttatccc cacttgttta tccccaaaca
    21001 aaggtggtct atgcaataaa atgaacgtca aagaaaaacg aatgaatgct cgttgcttat
    21061 ttacaaattg ttttatttag aatacacaga cagatttatg aaaagaaaaa gcttttcgac
    21121 acgattatct gttccaaaaa atgtctaatg aatgctgaat aagacagaaa atcaatgaaa
    21181 ggaaaaggac tagtttatta tgaaaaataa aaaaacgata cgtttgctag ctgctacttg
    21241 gctgattgct ccgtctgttt tgtcgaccaa taccgtattt gccgtagata atcaaatagc
    21301 tgatcaaaca gtcacaacag atacgactac atccagctca gctactagtg aaacaacgga
    21361 ttcttttaca gaagacccaa ctggaacaac taactcaaca acaggatcga ccgagccaac
    21421 tgaaattgat ccggctgatc catcagaaaa tgaaaagcca gccgagacat attcccttac
    21481 aattgctcct tctttgattg gtaagatcca actgactgct tcgacagcgg attcccctga
    21541 acagcaacct ttggatatta cggaaagtgg aacggtagaa ggtttattag cggaaacgaa
    21601 ggtaacttac actattacgc cagctgaagg tgaacaaatt gcttcagtcg ctgttaatgg
    21661 tgccaaagaa gtaaattatg atgcaaagac ctttacagta ggtacgaaca atttatctat
    21721 tactgcagaa tttaaagctg gaccggatac ttcaacagag aaaccaactg atcctagtga
    21781 agaagtccct agtacaccag atccggaaac gccaaacaaa ccagatgtgg gtaataacaa
    21841 caataataat ggttcaaata attcaaacaa taataacaat aattcgaaca gcgggaattc
    21901 ttcgaatcaa acaaacccat ctggatcgaa taacaatgga aatactcaga gagaaccaaa
    21961 ccatccaaac aatggttcat cgaatggcgg aacgaacaat gcttcatctc gtccaaatac
    22021 agcaaatcaa agcggtatag agaatccatc ttctgcttct tctgattttg ttgtaaaatc
    22081 accaattgat gctgtattgc ctacaaatac aacgaatgtc caacaagcaa tcgttcgaga
    22141 agcattcaag catcttggaa aaccatatgt atggggagca aaaggtccaa atacttttga
    22201 ctgttctggt ttgacttatt acgtatatat gaaagcaacc ggtcattata tcggtggctg
    22261 gacaggcgaa cagcaatatg caggtacgca gattccagta agtcaggccc aacctggcga
    22321 cctcgtcttc tggggacctt cttccggtgt tactcaccat gtaggtatct atattgggaa
    22381 cggacaattt atccatgcgc ctcaaccagg agataaagtt cgagtaactt ctatttcaga
    22441 tttcacgcct gattttgctg tccgagtaaa cttagcaggt ttacctgctg cttcaggttc
    22501 gctgacaggc ggctcatcta ttttagatgg attagatggc tcgttccatt tcagtcaaaa
    22561 ccaaacaact gatcagttct tgaaaaagat tgcagatgat gcacaggaaa tcgggcaaaa
    22621 agaaggaatc tacgcatcag tcatgatggc tcaagctatc cttgaaagtg gatctggaaa
    22681 cagcttatta tcaagcgaac caaatcataa cctttttggt atcaaaggat catataaagg
    22741 aagcagcgtg accttcaata cacttgaaca agatagttcc ggacaaagct accaaatccg
    22801 cgcacaattc cgtaaatatc cttcttacaa ggaaagtttg gaagactatg ccgacttgat
    22861 caaaaatgga ctgactggca atcctgattt ctacaaacca acttggaagt cggaaacaaa
    22921 agactacaaa gaagcaacca aatatttgga aggacgttat gctacagatc gccaatattc
    22981 acaaaaattg aacgcgatca tcgaagcgta cgacctgaca aaatatgatg aaccgaaaaa
    23041 agaagcagca acaactgaag aagcaaacgc tgaaatcctc aatatgtcta agcgattcga
    23101 tgtgccaatc aaatggacaa cagatggcgt gccaatcaca ggattagaat tttcaacgat
    23161 aaaacgcgtc aacaacgctt cttccatctt ccgtgtcgct tcaatctggg atttgtggaa
    23221 caattttacg gcaagacaga taccagaaac aactactcgt acagttaaag ctgcaactgt
    23281 tcctgtacta accttgttaa gtttagacaa atcattctgg cttgatctgc ctttaaagaa
    23341 atagaataaa ttaaaaaggg gatgtgacac tgtcacactc cctttttaat ttattctatt
    23401 tctttatatt tattttgtat tccgttacaa actcgcgtac ttggttaggt tccagagtga
    23461 catctccaaa actttcaagt cgctccgctc ctggcagttc ttgagtctca aatgtgatac
    23521 cgccatgatt tactaattta ttcccgcgca tttccggtcc atcttctcca aaatttgctg
    23581 taaaaatcac gattcctgaa gcatctgttt ttacttggat cgatactttt tcatctgggc
    23641 ttgtcaattc cgcagcattt agtaaaccag attgtttttt caacaaaaat ggatgatcga
    23701 tgccgcccac tatcattgat tggcgaaaat cactggcaaa gacctcacct aattttttag
    23761 gacgttggaa gtcaaatgct gtgccactta ctgaaactac ttctcctgta ggcgttgtat
    23821 ctttattgag cgtagcaaat aatcggctat tcaaccaaag tatatgttcg tcgattgact
    23881 gtgctggatc accggttaaa ttgaaataga catgatttgt aggattgaat actgttttgc
    23941 ggtcactgat cccttggacc gtcactcgcc atgtatcatc gttcgtcagc gtataccgta
    24001 cttctacatc taaagttcca ggaaatccgt tttccatatc tgggctagtc tgatagaaaa
    24061 tgaccgatgc ttcgttttca ccattgatga ctgtataatg ccactgcttg gcttcaaagc
    24121 taggtgcacc accatgtaaa caatgaccag tgtccgggtc tgtttttact tcgaacgttt
    24181 catcatctaa tttgaatgtg ccattcgtaa tccgtccagc catacgtccg attgaagcac
    24241 cgatataagg gtctttttct agatattctt ttgcttcatc aaaacctaag atcaactctc
    24301 gtttttcatt atcgattatt gtttgaaaat gaacaacccg tgccccaaga tcactgacag
    24361 acaaagatgt cccattttga ttcgtgatcg taatcaagtg atagttttcg ccaaaatttt
    24421 gttcattgat ttccatgttc cttgcaccct cttttctttc atttgcactg attccagtat
    24481 acatggaaac cgttttctta acagcttatt ccgtgagtaa cgagaaaaaa tttactatat
    24541 tctaataaaa gaccgtgaaa aaaaggttgt gacaaaaata ttgtcacaac ctctttttcc
    24601 gaacaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
    24661 gaggagctat ttttgcttgg ctgttcttat tacttgatgc aggacagctt tttcacaatc
    24721 tctttttcaa tacgattcca gtatataacc atgtcgtcag tacatagtag atgaaataca
    24781 atacgaagaa tatcgtaaac ggaacccaaa tatctttgta tggattttgc atgaatagac
    24841 caaacatttg taatccgaat aagacatgga ctacacctaa gattcctggt gctaagaata
    24901 acacgcctac ttctctacgg atcgattgtt taagcaaccc gcgtcgtgtt cctatctttt
    24961 caagcatttc ataacgagca atgtcactct tgcttcctga caagatcttg aacatcaaac
    25021 agctagcaag catggtcaag aaagctaaac ctaggaagaa tcccataaat tcaaaaccag
    25081 agaatagacc attatacact tcatacattt catatttctg gttgctgtac tctacttttt
    25141 gcaaagatgg attgttcatt ttattttgag caacaagtgt ttcgattggt ttcaggtttg
    25201 cttggaagtc ttttacctga actagctgta aagtggattg tggcaagttc agttgatcaa
    25261 attctgattg actgactacc ttgatttctt tttcttgctg ttccggcaat tcatagcttc
    25321 gaagttcctc ttgtacagtc agttcattca tttttttagc aggaactttt tctttcgtga
    25381 tcttcggcgg tgttccatct tcatgtttta tcatgatcaa tggttgagcc gcaaattcac
    25441 ttgcattata gtaaatcgtt cccgcatctt ctttttgagt ataagaagca gaaagtgttg
    25501 gtgaaagttc tttgattttt tgctgatctt ctgcccgagg attattcatc actagatcat
    25561 atccagaatt gttttctgtc aagtctgcaa tttcattttt aaaacctaat cctaccgtca
    25621 aagcgcccag tgccaaggca aacagcatag cgaccattga gagcatttgt gtatattcac
    25681 gaatacgaaa gtttaactgt gacaatgtga aattgttcaa ttttttcaat gaaatctgtt
    25741 ctgatttctt tagtaatgat aaaaagaaaa tgatcacaga atggaaaatg aaataagttc
    25801 ctaatacgat cgtaactaag gctaccccta taccaaagac ttgataggtc cccacgtgtt
    25861 tcatcatata gtaaccgcag cctagaaaag caattcccag tactacttca aggaaaaaca
    25921 agaatttatg ctgcttgatt cttgttggtg tctgatccgc acgaatcaat gctaaaatcg
    25981 gttttttgac aatcgaaaaa gcattgacaa cagcagctaa taaaaacaaa acagcaaaaa
    26041 acaaaacagt gatcaacaac ccgtgaatac taaaaggtgt aaaatgcgaa atattgagat
    26101 ctaactgatc cactaataat cggtggacga tacttgtcaa tcctacaccg acagctgaac
    26161 ccactaaagt agctgcgatc cctactaaga atgtttcaga aaaaatcatc tgtgcgattt
    26221 ttctgccttt tgctcccagc atcataaaca ttgcatagtc tttttgccgc atcgtcatca
    26281 aaaaggaatt tgcatataaa atataaacga atgtgatgat ccctagtaaa acagaaccga
    26341 atcggaaaat gataactgta gccgcaacga tcgtattact tttcaaaaaa gcttcgttgc
    26401 ttgccattga ttcaaacatg taaaaaatag cagatgccat aactaatcct gagaataaca
    26461 ccaagtagtc tcttaaacgg ctcttgatcc cagtaagtga taatttccaa agcatagtca
    26521 atctccccct actgttccaa gtttccaaga atagatagta tttctcgata gaaggaatca
    26581 cggctttgat ctccacgttt cacttcttga tggataccgc catccttgat gaataaaata
    26641 cgttggcaat aacttgcaga aaacggatca tgcgtcacaa gaagaatcga tacatgatct
    26701 tttgtattca attcgtccat cgtatctaaa agatcccggg cacttttgga atccagcgca
    26761 cctgtaggct catcccctaa tagaatcgtt ggttgtgtga tcaatgcacg tgcggcggcg
    26821 acccgttgtt tttgtccacc ggaaatctca gaaggatagc tttccaaaat atgagagatg
    26881 gataaacgtt ctgcaatttt ttggacacgt tgcttgattt cttttggtct gacgccttgt
    26941 aaagaaagag gaacagcgat attttcttgt gctgtcaagc tttctaataa attgaaatct
    27001 tggaaaataa agccaatctc attcgcacga aaatctgcta gttgatttcc ttttaatgtg
    27061 gtcacatctt tttgattgat tcggatatgc ccatctgttg gtttatctaa tgtggataaa
    27121 atattcaaaa gtgtcgactt accagaacca gaagcgccca tgatcccaat aaattcgcct
    27181 ttttctacat cgaagctgat gccgcttaat gcttgcgtct ttttctcatt gttttttccg
    27241 tatacttttg ttacattctg tacttcgact attttgttca tttctttccc taccctttaa
    27301 ttagtttttc cagtgctgtt ttcggcacgt cttttttttc gacttcttca tacgaagtga
    27361 cacctttatt tttgttccag gtgattttta aatatgctcc tttacgaagc ggtcgagtca
    27421 attgtccctt gaagtccagt tctttttcct taccctcttt cgtatagcca ggcaggatat
    27481 atctatagtt gactgtttct tgtccagagt ctgattttga aactgttttt gtaccatctg
    27541 tcgtaatttg tacatagtat tcttctccac ccataactac gtaatctgcg attttcaccc
    27601 ctgcaagtac cacaacgatg aaaacaagta gaccgatcag aagttttttc actttttccc
    27661 cttctctctt actttatata ctgattatag aaaaaagcga acaattctgc cttcgaatca
    27721 actatcattc ttctttcaat tttgtcattt tctttaaaac aactaaaaaa agagttcaag
    27781 acaataatcc gtcctaaact ccttcttcct atcttacaaa aagtatgtca cttacggtct
    27841 ttcatgtcgc ctgtattgat aacaggttgc gttcctttgt ctgtaatgat tgagacaagt
    27901 aaattattga tcaattggac tttccgatca tcggtgaaat tgatttcttg tccatcttcg
    27961 atttgctcta aggccatttg agtgattgtg acagctcctt caacaatggt ttgtcgagca
    28021 gacaaaatgg cttttgcttg ttgacgttgg agcatggcac tagcgatttc tgttgcatag
    28081 gctaaatggt tcaagcgtgt ttcgattact tcgacacctg ctactgctaa acgctcttgc
    28141 agctcttttt gcaactcgtc agacactgcc gtcgtgtttc ctcgaagcgt caaatcattc
    28201 tcattgaatg tatcatacgg gtattggcta gcgatatgcc gaatggctgt ctcactttgg
    28261 atttctacaa aatcttgata atacgctaca tcgaataatg cctttgctgt atcaacgact
    28321 ttaaagacta ctactgctga gatttcgatg gggtttccat ccaaatcatt gacttttaaa
    28381 acagaactat tgaaattccg aactttcaac gaaacagtca ttttttgtgc caaaggtatc
    28441 gtaaggaaaa agccattttc tcgaatcgta cctaaatatt gtccaaaaaa caaaattact
    28501 tttgcttgat taggactgac tacagttgcc gaacttaaca acaataacgt gatcaaccaa
    28561 agaaaaatac ttaaaaaaag tgcccaaact ttcgcttgcc acatacctaa atagaagaaa
    28621 aacaatccaa ctaaagctaa tacgaccaat ccgacaatcc ctgcataacc attgatataa
    28681 aatgctcttt tttctctcat gctacttcct gcgctccttt atccttcaat aaatatctaa
    28741 tgttactttt ttaatttttt tttcgaatac gcgccattac accttctagt tctaactctt
    28801 tgtcttcagt gatgtaccaa ccgcttgcct gtgtccctcc ggctgcctgc ggtaaataat
    28861 ccaattttgt tcgaacccag tgcttttctt ctgtaaaata gtcttctacc aagacctcta
    28921 gatccatccc tgctgaaaga ggcaaatctt cccgaacaga tttaaagata aatcgttcct
    28981 tcttcccatc atacttcaat attcctgtat cgataatcgg taaagaataa taatccagct
    29041 ctttcttcac cgtcaataaa tgtttgaaaa cttcatggta cacttgctct aattggcgac
    29101 cttctgaatt gttcgggtaa ttaaccatca ctttttcttt gtctggatca tagaaggttt
    29161 ggcttagttg agtgatataa gtatgtaatc tttccatttc tgtttttaac ttttctcttt
    29221 gaccttcttc taccttcata tactctttca ccctgttttc tttttagata ttttctttct
    29281 tattttacta tagttatgtg cgttaagcga tgaaaaacag caaagattgg aaggatcttt
    29341 gctgtttttt cttaaaatat ccagttgagt acaggatcaa tctgtttcat tgtcctcttg
    29401 atccgtcgta atattgatcg atgtgtcttt agcagtgatc tttagctttt tctgtccttg
    29461 ttctaacaaa gtatttgtta ttggctggta gtctttttca atataagatt caccagacgt
    29521 ttggatagta tagccactgt tttccatctc atgcaaagat aaattgccac cgtttaggat
    29581 gacttttcct tctttgctga tcaaactgtc ttctagatgg aagttgctgt tttcacctgt
    29641 gacgatcaag ttatccacat tggcctcatc taatcgaagt gagcctaatt catctttcgc
    29701 agttattgtt cctcctgtaa attcattgaa cgatacccag ccatcatgtg tcttagtatt
    29761 cacttttttg attgatactt cgttgatatc aattgcacta ttattccctt cgattgtcaa
    29821 ttcagataat tcatgatctg cagggacaac aatagtgatt tgtgcacttg tgtcttctaa
    29881 actaatgatt gcacctgttg attttttgac tgcattcaca gtcaacacgc catttttctc
    29941 ctttatttct ggcttttctg tcttatcata agtcgttttt atctcaaaat ccaatccgcg
    30001 tatgaccgaa actgtttgat tttctgcatg gatgacgatt ttgtttgcat ccttgatttt
    30061 ttttgtctca gtaaccatat cgatcaaccg aggaccatta tcccaagtca gactcttttg
    30121 agcgcctaat gtataagttg cacctgctag gaggccacca gtaagcatca gaccaatacc
    30181 ggtaatcaaa aaatgtttca atctcaattt atgtgtcctc cttcaaacgc ccgctgaatg
    30241 cagatctctt tttgtaataa aacgtcgtcc catccattta acaaagttca tcatgacata
    30301 aaaaagccct tttgttcctt tcagcaacag tggcgaaata aatactgcgc ttcctgtggc
    30361 gaacagaccg acaccgataa agaataatcc actcagaatc gattgtccaa acacactgat
    30421 tcctgagatg atcatgaata ggccaacacc taagatactg atacataaaa catagatact
    30481 aaaaatgagc gctagtaaag caaccaatag tcccccaata accaatataa accccagtgc
    30541 tagtggaatg aggacaggag aagcaaataa accgataatg attaaccaaa tcaggcgaat
    30601 ttgtcttttt ggatttttca ctaactcgtt ttctgcttgt tcttcttctg aaaagtaact
    30661 gatttttaat tttaaggcaa actgctttgg tgtaccatat tcatcaatga tctgatctaa
    30721 tgtttttcca gaatctaaaa aatattcctc ataatactgg atcaaatcaa aacgatcgtc
    30781 ttcaggaatt tcttgaagat gatccacaac ttcattcaaa tattcattca tttgtgctct
    30841 cctccaataa ccggtctaaa gaatctcgaa aaacaatcca ttcatcaata atttcttcta
    30901 aatgacgact tcccattggt gtcaacgagt aatatcgacg gtttctgcct tcaaaaggtt
    30961 catcataggt ctcaagatat ccttcttttt tcaatcttct caaaaccgga tatagtgtag
    31021 attcactgac agagagactt tcttgtacga tttttgttaa ggcataccca tacatatctt
    31081 ccttgcttaa tgctgctagc acagtaccat caagaagaac agttggaatc tggatgtgca
    31141 ttacgtcacc ttctattctc aagtgtaata ttttatttca atctatatta tacaacatat
    31201 aatatagttt caaaatcagt ttgtcaaata caaaaaaact gactaaaaga tttgatcctt
    31261 tttagtcagc aaaaaatggc ttctctttga tttttattat aacagtggca taatccaatt
    31321 tgtccacatc cagctcagtg gcatgataac gatcattgca ggtatcatgt cagctactgg
    31381 gaacattttc acgttgatca ttctaaaacc ggtagcgacc atcaagaatc cgccgcaagc
    31441 tttaaaatct aagatcatgt ctggtgtagt caatggataa ataaattttg ccaataagaa
    31501 caaaatataa aaaataatga attggggaat tgctatcaca gaaacgacat accctaaatt
    31561 acacgcaaaa atagctgctg tgaaaaaatc aaggacagat ttagaaatca aaatcgtact
    31621 gtctcctgtc atcccagaat ctaaacttcc atagatcccc gtaccacttg cacaaaatag
    31681 aacaatgacc gtcactaatg tgctgataaa ttcatcgtgg gtcataccaa gcttttcact
    31741 agggaagatt ttagaaatag gtacttgcat cagcgcagcc cccttattta tcccattacc
    31801 taaatgaaca atcaaaccaa tcgctgttcc tatcactaaa gcaaaaataa cagccggcat
    31861 atttttcatc ggcgcaatgg aatagattcc catccccatc gaacacacac caaaaaccat
    31921 cgtcagctgt gctttaaaat gttcacttaa tttatttcct actaatccgc caattagtcc
    31981 gccaaacaaa acggaaccta cattgatcaa aactccagtt ggcatcaaaa aactcctctc
    32041 tttttctacg aagcgaaaaa aatcagaaaa atttctgtaa ttattgcttt cgctgcaaga
    32101 aagattgtaa cagacagaga agattacacc aattcatttt ttgtgttaaa gtattcttta
    32161 ttggaataag taggaggaat atgatggata tccataaatt cgaagttttt cttgatttag
    32221 ccgaaacaat gaatttcacc aaaactgctg atcgacaatt caccacccaa ggaaatattt
    32281 ctaaacagat tctttcgtta gaaacagaat tagacgtcaa attattcgaa cgcgcccata
    32341 gaaaaatcga attgaccgaa gctggaagct tattgctccc atatgtaaaa aatgtcgtag
    32401 aacaatacca tgctatgcaa gaagtgcttg aaacctatac gaaagacaac aatctttcat
    32461 taaatatctt aacgataccg acaatgatca attataaagg tttttccaaa atcaccgagt
    32521 ttttgaaacg ccatcctgaa tttacagtcc agttgaaaga agtggaaagt gtcgaattaa
    32581 cttacaatga cgccaatgaa catagtgaca cgatccactt tgctagaagt ttccaggccc
    32641 caccaagttc gattgagtgg ttgcctactg aaacagacga ttttgtagca gttctaccta
    32701 aaaatcatcc tttagctagt aaaaagaaat tggatctaag cgaactgaaa aaggagaact
    32761 ttttgctcct tggccccact acgaacttgt atcaacctgt actagatctg tgtcgcgaag
    32821 ctggatttga accaaaaatc tcttataaag gagcaagaat cgacttgatc atcaatatga
    32881 ttgcaaatga catggggatt gctattgtta tggaaaaaac agtcaagaat ctattaaaag
    32941 aaaatactgt gattctccct atttctccaa caacagagag ttatcttgca tttactcgta
    33001 aagtcggtga gcattctttg gcttcagata cattttggga gtatttaaat caagaagaaa
    33061 actaagccct acattttctg tccatctatc cacccaaaaa aagagagcga cgcatgactt
    33121 tcgctctctt tcttgcatca tgtccttgtt tcaatgacac ctctaacatt tctcgtagtt
    33181 caataagaaa gcagcgattt ttatgccaaa agaaccgtaa gaacatttcc ctaatcacaa
    33241 aaaaacgact aaaaacccct aaaaaccttc aatagaattt ttaggggttt tattcttttt
    33301 cctattgttt acttaaccgt atcattgctg tcgttcctac tccttcgata ctgctataat
    33361 caatacgtcc atcatgcagt tctagaattt ttttaaccag tgaaagtcct agcccattcc
    33421 cagaaatttg acgacttgta tctccttgat aaaatttttc aaacattcga tctctggtct
    33481 cttcattcat tcctacacct gaatcggtca cttccagtac aatctctgtg gctgtctcaa
    33541 atagtttgat atgaatctgt ccattgattt gattgtactt tatggcgtta tccatgatat
    33601 tcagccacac ttggtacaaa aactcttcat tgcctgtgta atttactgca gcaagttcga
    33661 tatccaattc tagctgttct ttctcccatt taggctgaaa aaaaacgatt acttctctga
    33721 tttgttcatc taatcgatac tctttctttt ccagttggat acgctggttt tccagtttgt
    33781 tcaattttaa gatgttctct gttaattgtg aaagctgcgt gatactttcg ataatccgat
    33841 ataaaaatat ttggcgttct tcgtcagaga gatttggggc ttgtaacaat tgaacatatc
    33901 cttggattgt tgctagtggt gttttgaatt catgggatac actggacaca aagtcattcc
    33961 tcagtgtctc gatactattg agttcttgga ccataacgtt gaaatcttta tacagttgct
    34021 gtacttctgc tactttctgc tgttcatcca ttcgaatcga aaaatctccc gttgctacca
    34081 gactcatatt tgtccctagc gcactgattg gatgcaggat acgccgccca acaaaaatcg
    34141 atattcctgt acccacgacc atactgaacc ccgcaaagac gaataacggg tataacgttg
    34201 gttttccagc cgattcagca ggcgagatag aatgtcgaga caataagaaa ataaaaccga
    34261 gaaaacacag cagtgtcact aagatcgaaa tgaagacaat actagcaaaa tagatccata
    34321 attgtgaaaa tagctttttt ttcatcttta aataaccgcc ttatagccta gtccccgtac
    34381 tgtgatgatt tgaaaatctt ccgtcttttc aaaacgggtc cttaaacgtt tgatatgtac
    34441 atcgatcgtt cgttcttctg tattcgtatc taatccccaa atatcatcca tcaactgttg
    34501 gcgagtgaag atcttgttgg gataagacag taatttatac agaagcagaa attctttttg
    34561 aggcagaatc aacgaatgct gatcttgtaa cactgtataa gcatctagct ctaattttgt
    34621 ttgtccgatt atcaagctac tgctgtaatt gatctttgaa cgtcgaagca atgcttccac
    34681 acgtaggatc atctcattga catcgatggg ttttgtcata taatcatctg ctcctaattt
    34741 gaacccgcgt ttcttatcct caaaatcggc ctttgccgta atcattagaa caggtgtgtc
    34801 aaatctggtg ctacgtaatg agtctagcaa gtcatagcca tccattttcg gcatcatgat
    34861 atctgtaatg attaattcta tacgtgtcgc ttctagtttt ttcagcgcat cctctccatt
    34921 caatgcggtg atgactgaaa agcccactcg ttccaacaca gaacggtaaa gcaactgtaa
    34981 ttgcggatca tcttctacta ctaaaatacg attcattgat ctccctctgt tttttgaaaa
    35041 atttctagat tttttcttgt agttcatctt cagttaacat ttatttcgta tactacgatc
    35101 attgttctga tatacttatt agtataccga atatttcaat acgggagaag ggtgattatg
    35161 cttcaattaa aagatataaa aaaatattat aaagtcggcg aaaccaccac caaagcattg
    35221 gatggtgtgt ctgtcgcttt cagacaaaaa gaattcgtag cgattttagg tcctagtggt
    35281 tctggtaaaa cgactatgct gaatgtgatt ggtggactag ataattatga ttccggtgat
    35341 atggtaatca acggaaagtc tactaaagac tttaaggaca gtgactggga tgcctaccga
    35401 aacaactcta tcggtttcat ttttcaaagt tacaacttga ttggtcatct aggcattatt
    35461 gaaaacgttg aacttgggat gacattaagt ggtgtttcta aagacgaaaa acgaaaacgg
    35521 gcagaagatg cattgcaccg agtcggcttg acaaatcata tgcataaaaa gccaaatcag
    35581 ttatctggtg gacaaatgca gcgagtggcc atcgctcgag cactagccaa tgacccagat
    35641 atccttttgt gcgatgaacc aaccggagcg ttagatacag aaacaagtat ccagatcatg
    35701 gaattgatcc aagagttatc gaaagaaaaa ttagtgatca tggtcacaca taatcctgaa
    35761 ctggcccatc aatacgcaga tcgaatcatt gaattttctg atggtaagat cttaacagat
    35821 tcccatcctc acatcgaacg accaaaagat gatcaattta acttacgcag aacaaagatg
    35881 agtttttgga cagcgttaaa gctttccttc aacaatatcc gaacaaaaaa aggacgtact
    35941 ttcttgacct cctttgcttc cagtattggg atcatcggaa tcgcgatcgt tctgtccctt
    36001 tcttctggat tccaaaaaca aatcgataac acgcaagctg aaacgatggc gaaatttccg
    36061 attactattt ctaaggtaac gactaaccag actcgtgacg atgccggatt gggtgcaagc
    36121 aaagcggatt atccagacag caaaacgatt actgcaaaag tcagcgaaga agataaagca
    36181 cagcacacga ataagattga tcaaacatac gtcgattacg taacagacat cgatcctaac
    36241 ttaagtaata atatcggatt tacccgtact acaggaatca atcttttacg tgatgtaaat
    36301 ggcaaagtac agcctgtcag cttctcgaat caaaatcctg atacggaatc tctttcttta
    36361 tctagtacga tgtcagcaat gacaggtgtg ggagtgtcaa gtttccctac acagcttgac
    36421 actagcaaag aaaacttttt aaaagacaat tattctttac ttgccggaag ttatccagcc
    36481 tcagcaacag atgtcgtgct gatcgtagac ggcaataata atactaatat caacgcattg
    36541 aagaatcttg gatttgatgt gaaagaagac gaaaaacttg attttgatga tatcgttggt
    36601 acaacattca aattagtaaa caacaatact tactatacaa aacttccaac tgggaatttt
    36661 atccctaata cagattatga tgcgatgtat cagaatgctt ctgatgaatt gaagatctcg
    36721 gggatcctac gcgtgaaatc ttcttctaca atgaatctct tatctccagg aatcgcttat
    36781 agcgatcaat taacgactca aatcgtcaac gaaaacaaag aatcagaaat cgtcaaagct
    36841 caaagagaca gtggtgtaaa tgtcttaact actgaaaaag ttgacgaaaa tgccaaacaa
    36901 acattgctta gttatttagg tggagacagc ttgccttcaa gtatcatgat ttatccgaac
    36961 aattttgaag acaaagaaaa gatcttggat tatctagatg attacaataa aggcaaatca
    37021 gatgaagata aaatcattta tactgattta gctggaacga tgactgaact gactggcgga
    37081 ttgatggatg ctatcactta tgtccttatt gcttttgcag ggatctcttt agtaacaagt
    37141 atgatcatga tcagtatcat tacttataca tctgtgattg aacggacaaa agaaatcggt
    37201 gtactaaaag ctttaggtgc acgtaaaaaa gacatcactc gcgtatttga cgcagaaacg
    37261 tgtattttag gtatttcttc tgggatatta ggtgtgttta ttgcatggct agctacgttc
    37321 cctatcaata gcatcttata caacatgacg gatctaaaaa atgttgctca actaaatcct
    37381 gtccatgcga ttattctagt gatcgtctct actatattga cgatgcttgg cggtcatctt
    37441 cctgcacgaa tggcagcgaa aaaagacgcc gctatcgctt tgcgtgcaga ataaggttgt
    37501 gacaaccaca tttagatttg agtcataaga gcgctgaaat agtagaaaac agcaagaaac
    37561 gaggaagtaa tatgttcctt gtttcgcgct gtttttttta attatttttt ataattctgg
    37621 tatacttttc tttcctttgt accacaactc ttctggcgta tacctgtctt ttccatttat
    37681 gaatactgtt ccatctgctt caatcgttac ttcagtccca atccacatat tacgagaaag
    37741 tgcgagaaga ttttttcttt ttcctcttcc tttttttcca aattgcaagg catcggttat
    37801 ctcttctgtc caatgagatt tcacgtattt tacttctccg ttttcaaatg taactttcaa
    37861 aatattcgct atattttcca cttttactgc tttaggaaat gactcctcca tttttttcac
    37921 tttccttcct ccaacactaa gtatgcgctt tcttttagtg ataagtatat catagtttat
    37981 tctattatca aaaaaacgca tccattccat tgtagaatac tttaccgaac aagttgaatt
    38041 gttttcctct ttttcacctt ttactataga taggtaaaga caactaagga ggaatctctc
    38101 atgacagatc aaccgtatga tttaagaaaa gtcatcgaag aactaaaaac acttccaggt
    38161 caatatcacg aaacagatat tgaaatcgat cctgaagcag aattatctgg tgtttaccgt
    38221 tatatcggtg ctggcggtac agtaaaacgt ccgactcaag aaggaccagc tatgactttc
    38281 aacaatatca aaggtttccc cggcacacgt gtgaacatcg gaacaatggc tagtcgtaaa
    38341 cgtgtaggtg aaattttgca ccatgattat cgaacacttg gtcgtttctt gaaagacgct
    38401 gtagaaaatc caatcaaacc tgtgaaagta gacaaagcag acgctccagc acaagaagtc
    38461 gttcatctag ctaccgatgc agacttcgat attcgcaaca ttttgcctgc acctacaaat
    38521 actcctgaag atgctggtcc atacatcaca atgggtgtag tcttaggatc agatccagaa
    38581 aaaacaatga gcgatgtaac gatccatcgt atggttctag aagataaaga tacgatcggt
    38641 atgtacatca tgcctggcgg acgccacatt ggtcacttcc aaaaacaatt cgaagccttg
    38701 aataaaccaa tgcctatcac tatcaatatt ggtttagatc cagcaattgc cattggcacg
    38761 actttcgaac cacctaccac tccacttggc tacaatgaac tatggtgtgc aggagctttg
    38821 cgtaacgaac ctattcaaat cgttgatggt atagcagtcg atgaagtggg tattgctcgt
    38881 gctgaattca tcattgaagc agaaatcatg ccaaatgaaa caatgcaaga agatatcaac
    38941 acgaactcag gaaaagcaat gccagaattt cctggctata ctggggatgc taatccagct
    39001 gtacaagtag tgaaagtcaa agcagttact caccgaaaag acaatccaat catgcaaaca
    39061 acgattggtc ccagtgaaga acacgtatct atggccggta ttcctacaga agcaagtatt
    39121 cttgccttgg tcgataaagc tattccagga aaagtagtaa atgtctacaa tcctcctgca
    39181 ggtggtggta aattgatgac catcatgcaa atccgtaaag aaaacgaagc ggatgaaggt
    39241 attcagcgtc aagcagctat tcttgcttta tcagccttca aagaattgaa gacagtcatt
    39301 ttagtcgatg aagatgttga tattttcgac atgaacgatg tagtttggac tttcaacact
    39361 cgtttccaag gagataaaga tatcatcgtg atcccaggaa tgaggaacca tccattggac
    39421 ccatcagaat taccagaata taatcctggc tctattcgtt tccgtgggat aagtgcaaaa
    39481 actatctttg acggtacagt tcctttcgat atgaaagaac attttgtacg tgcaaaattc
    39541 caagaagtag aagattggga aaaatatttg aaataatgaa tgatctgcaa ctgttttgac
    39601 atcataaaaa ggagtgcgga aaatggaaca attggcacaa cggaagaaaa aaatcgtgat
    39661 tggtgtaagt ggcgcttcag gcaccattta tgccatcaac ctagtgaaga aactaaaaga
    39721 atatccgatg atagaaacac acggaatcat cagtccatgg gcaaaacaga acttaaagct
    39781 ggaatccgac ttatcactaa ctgaattcga aagcctgttt gattaccatt attcaaacaa
    39841 agatttaggt gctgctgtcg ccagcggatc atttttgacc gatggaatga tcatcgtacc
    39901 ggcaagtatg aaaacagtcg caggtatctc tgttggttta ggcgacaacc tgattgcacg
    39961 tgctgctgat gtaacaatga aggagcaacg aaagttagtc attgttcctc gtgaaacgcc
    40021 attaaatgcc attcacttgg aaaacttgac taaattagca cgactaggtg tgcaaatcat
    40081 accaccaatt cctgcttttt ataatcagcc tcaaacgatc caagatcttg ttaatcacca
    40141 tacaatgaaa cttttggatc aatttgaaat tgtaaatgag cattcaaaac gttggcaggg
    40201 tgattaatat ggaaaaagaa acgatagttg aagtaaccaa actaggtttc acgatccaag
    40261 ctgctgttaa agtaatcggt aaagacttat tgatcacttt aacaggcgga gatacaccac
    40321 atatcggaac agtcactaca ttttctcgtg atacagagat gcaaactgta cgtttcccta
    40381 gccacgatgg tcggttccat aaagacgatg tcttagccga aaaaatagct gtgatcatca
    40441 aaactgccgt tccgggaagt tgtacgatca cttccggtat ccatgttgac catatctcca
    40501 atgcacaaat caacgcctct tttccgatgt cagaagagtt aggttacaag attttagatt
    40561 ggttggaaaa aatagatttc catatcaaag aaccagttta ttataaagat ggtgaaaaac
    40621 cattatgaaa gaagaggttg tgaaatcagc gttatgacct gagtatgaga gaaaaaaatg
    40681 gaaaaatagc ttctcatatt ttgccgtttt tgagctaata ccgagggaca gctgattgaa
    40741 cgccgtttat caagaggttg tgataaagcc gcttcgtttc gagtagtaag aaaaattttg
    40801 taaacatggg tttccacatt tttgcataaa tttgacttac taccgagaaa ctggcttttg
    40861 aacaccgttt atcaagaggt tgtgacacgt cttttgtcac aacctctttt atttttgctt
    40921 cactgtaact gctacaatat caccgatttc ttttccgatg tttttacgaa tatcttttcg
    40981 tagtccaagg atgtagcaga ctgaaccatc tggatttttc acgcccatat tcacgatact
    41041 gccttcatac ggcacattgt caaaagttgc ttggacttta actcttccag caccgaattc
    41101 ttttcttata tcatacggaa aaataatata tgccccgccc ttgccgactt cggaagcata
    41161 gatttttgat tcaaaagaat aaatttccct cattttattt ctcctatttt ttacatctag
    41221 tatagcatag caaaaaaaag aaggaactgt gacaaaagtc ttgtcgcagt tccttttttc
    41281 gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatagtc aacaaacatg
    41341 agaagctgtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct
    41401 cttccttaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc agatgaaaaa
    41461 tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag ctttttcaca
    41521 acctcctaca tggtgttcaa aatattattc gccttttgct tctttcacta attttgcgaa
    41581 tgcttgttgt tggaattcag cagctaaggc tttcgtacgt tgttcttgct ctgctttgct
    41641 catttttgga ttttcagtca ttgcttttac cataccgtct tgaacataag cttttgcttc
    41701 actttccatc gtactttgct gtttcttcac taattcagct aacttggctg cttggtccgt
    41761 acttaattct acccatgtct ctgggatttt gaatgtattc actcggctgt catattcgtg
    41821 attgttatca gcaatattga accagaattt atacatgtcg tttttgtatt cccaataagt
    41881 agttttcgtc actttttcag catcgccgtc tgtcttttct actttatttg tttcgttatc
    41941 cgtaccagtt gcttttggtt tttcttgttt ttcatttgtt cggtaaagat acactttttc
    42001 ttttccattc cctaaagctt tatacaaaag catatctgct ccttgggaat cagcactgga
    42061 aacaattttt tctgtttttg tttccgttac tttttccatt ccaaagtgat tggacaaatt
    42121 agcgacgatc aaacttagtg aagcgacgaa gattagtccg aaaatcaaag ataaaaaggt
    42181 ttgccatgtt tttttagcga acacgttcgt taaagcaaaa gcaagcaagc tgacaatcaa
    42241 aactccaata atcatgcatt atcccccgct ttcattagaa caggttcttt tttcttcaat
    42301 aaaaaggcaa taataaagcc aagaatccca aagatgatcg ctacgataaa cgcggcatgg
    42361 taacctgaca gtgtggcatt gattgcctga tctttataag ctaatggcgt attatccaat
    42421 acagatttgc ttgggatatt tgcttttgtc acattcgtca acacactgat caaaatagct
    42481 gtaccaatcg agcttgcgat ttgacggaat gtattattta ccgctgtccc atgactgatc
    42541 aagttatttg gcaatgcatt cataccagaa gtcgttaccg gcatcatgac catggagatc
    42601 ccaaacatac ggatcgcata aaggatcacg atatataaaa ctggagtcgt ttttgtcaag
    42661 aaagcaaatg gtgcagttgc cgctgtcaaa atgaacatcc ctgtgatggc taaacgtttc
    42721 gcgccgtatt tatcgaaaat cgcacccgtc acaggcatca tagcccccat gatcaatgca
    42781 cccggcaaca acatcaagcc agaatggaat gctgattctc cacgaatatt ttgaatatac
    42841 aatggtacga ccatttctgc ccctaccatc gccatatttg ttacaccagc taaaacagct
    42901 gcaatcgtga atgtagatga tttgaataca cgaagttcca agaatggatg ttccaaatgt
    42961 aattgacgcc atgagaacaa ggcgatgaaa atcacaccaa caactaagaa accaattact
    43021 ttcaagcttc cccagccgtc atctcctaca ctagaaaatc cgtaaagtaa actaccgaat
    43081 ccaatcgttg ataaaatagc ggataaaata tcaatgctag gatttgacaa tggaataaca
    43141 cttttcatta ggaagaatgc taaaaccaag acaagaacaa cgattggcag taccatacca
    43201 aacaagtcac gccaatggta gttatcaatg atataaccag ataatgtagg gcctaaagca
    43261 ggagcaagtc caataacgat ccctgacatc cccattgctg atcctctttt ttctggcggg
    43321 aaaatagata gcatgatatt ttgcaataat ggcatcgata ctcccacacc gcaagcttgg
    43381 atcaaacgac cgattagtaa tgtactaaaa ttaggtgctg taaagcaagt aatcgttccg
    43441 atcaaaaaga tcgtcatggc agaaagatat aaatttcttg aactgaattt attgatcagc
    43501 catgctgtta ttgggatcat gatcccgttg actagcaaga aaccagtcgt taaccattgc
    43561 acatcagacg ctgaaatatc gaaagctttc atcaatgtag gaaaagctgt cgtcaataag
    43621 gtctgattca aaacggtaca aaatgtacca attaataaga caacaactaa aagtgtccga
    43681 ttatatggtt ttccgtaaat atctacgggt tggttgttac tcatatttct ccttctttct
    43741 gaattttttc tgaaactact agaatacttt agtcctaaat tcgctatttg tcaacctact
    43801 tgacacataa gttaatacgg atataattaa gggaagcaag aggacaaaag gagtgacaaa
    43861 aatggttggg gcttccctta aacgtttgtt ggaaaatgat caattggttg ttggcatcag
    43921 tgaattaagt gatatcgtag gtgtgtctcc acgtcaatta aggtattggg aacaaaaggg
    43981 attcattcgt tcgatcgaag ctgatgcgaa ttgtcctaga aaatatcgtt tacctacagt
    44041 cataaaagta gaactgatca aaaaatattt agatgacgga tttactttgt caaaagctgc
    44101 agaaaaggca gaaatccgcc aaaagaaaat gcaccatgtc cgtaaagttt tttcaaaagc
    44161 aatcaaagat atcgaattga tcgacgaacg ctatacgatt ttacaaatcg gtaacttcaa
    44221 agaagagaat gctaagatgt acatcattca tgacaacgag acagacgaat taacttacaa
    44281 gctccttcct gtcgatgaaa aagctgacta cgaacagttg ttaaaagaac tctaaacgcc
    44341 actaatctcc taagtaccaa gctttttaat aagtagatgc tacataaata cttgaaaaaa
    44401 ggcctgtttg aacataagaa tatccgaacg gtgatctgcg tttctttcat atagaaagga
    44461 attctccatc actgttcgga tattttttta tttttttcgg ttactttctt ctaatacatc
    44521 tgttagccaa tttgcatatt ctgtttcccg attgatcgca tgcgctaaaa tcaggtagtg
    44581 tccgtaattt tcatgttttt ctttttcagt aggaaagatc gttttttttc gctctaatag
    44641 atgaagtcgt ttttcttcat gaaggtcacg ctgctgctga ataatttcta ctaatgccgg
    44701 atcattcacg tctttgacga aatacaattt cagaacaaat tcgtctcgat taaccggtag
    44761 ttcattggtg ggtgtatgaa tccactctgc caattgttcc ttcccttctt ctgttaattc
    44821 ataaactttc ttttccaatt tttcacctgt gatctctact tgatgctgaa tgattccttg
    44881 ctcttccatt tttgcaagtt caggataaat ctgactatgt tttgcttgcc aaaattctcc
    44941 tatttcattt ttgaacgatt gattcaattc atatcctgtc atcttcttct tacttaataa
    45001 ccctaacaaa atatatcgaa gtgtattttt tcttgccatc agttttccct ccttgacaaa
    45061 tctttaaatc cattctatca taaatgagta atcgaaaaca tgtagtttat tacatgtttt
    45121 aatttataag gagatgctta aacatgaaag aatttaaaga attggaagat tttattggaa
    45181 ctcactttat ctatacgtat gacaatggtt gggaatatga atggtatgcc aaaaatgacc
    45241 atactgttga ttaccgtatc catggcggaa tggtcaaagg ccgttgggta aaagaccaag
    45301 aagctgatat cgttaaattg acggacggcg tattcaaaat cacttggaca gagccaacag
    45361 ggacagacgt ggcattggac tttatgccaa atgaaaagaa acttcacgga atgatctttt
    45421 tccctaaatg ggttgaagaa catccagaaa tcactgttac tttccaaaac gaacatatcg
    45481 cagaaatgga agcagctcgt gaaaaatatg aaacttatcc aaaattactt gttcctgaat
    45541 ttgctacgat cacttatatg ggtgatgcag gtatagacaa tgaagatgtt attagcgaag
    45601 aaccatacga tggaatgcca gatgatatcc gtagcggaaa atacttcgat gaaaattata
    45661 aacgagtaaa ataaggttgt gaaagagagg ttgtgaaatc agcgttctga cctgagtatt
    45721 agagaaagaa acggaaaaat agttcctcat gttttgccgt tttttgagct aataccgagg
    45781 gtcagctgat tgaacaccgt ttatcaagag gttgtgaaat cagcgttatg ctttgagtaa
    45841 taagaacgct agttagaaaa tagcttttca tattttctag gctagtgggc ttattaccga
    45901 gaagcagccg tttgaacacc gtttatcaag aggttgtgaa atcagcgttt tgacctgagt
    45961 attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg
    46021 agggtcagct gattgaacac cgtttatcaa agattgtaac aactgcatat tcacacagtt
    46081 aaattagtga cttcaaaatg aaataatgct cccagcaaaa aattaggctg ggagcattat
    46141 ttcgttattc tgttcatcaa aaatcagcgt tttgattcca tttcatcaca agttcctcct
    46201 catttactgc ttcatctttt tgctgtgttt gaattgtaaa accttgtgaa agatagaaat
    46261 gaatagctga ttgatttttt tgatagactg tcaaagtcag tgttttattg gtttctttta
    46321 tttttttcat tagttgcgta ccgattcttt gttgccggta ttcccttttc acaaagattc
    46381 ctgcgataaa ctgatcaatc accccagcaa aaccaataat ttcttgatct atttctgcta
    46441 agtaaatcac tgcatctggt aaagctttcc taacagattc tacttgattc tcccaataag
    46501 ttgatggaat gaattcatga gaatccaagt tagcatgcag ccaaatcgat agaacagatt
    46561 ccagttcttt ggcagacaat tttgttgttg tgcggatcat tttttctctc cttacttatt
    46621 cattgatttt aaaataaaaa ccattttttt gaagctcttt tacaagtttt cgcccctcaa
    46681 aaaagtatat cttctcttgt tttacttttc taataccaaa agaaacagca actttaggat
    46741 catcagctga aacgattcga taagttccac caggcaattg atccagcttg actttcccat
    46801 gatggtcgct ttgtactgaa aacggcacac catttctagt aagtgttttt ttcttatgtt
    46861 tcaaaatgaa tgaactacca tttaccggtt tccctgacga atcactaata ataaatgaaa
    46921 aatcaagcac ttttttactt aatctatact gatagattag tcgtccgata aacactaaca
    46981 gtatgactac agctattatc gttcctgcaa gaataaacca ttgccgcatg gttctttcct
    47041 gatttccttt tccttcttct ttttttaccg attcgttata aggaatccga tgtcctgtca
    47101 ctagcaaacg atgcgtgttg atcatatacg gcgtacatgt aagcaaggta actaagtctt
    47161 ctccttctga aatcgttaga acagacgtgt catctggcag taccacttgt atatttttaa
    47221 cttgataagc caatttctcc cccaaaacag tcaaaataaa cgtgtctccg ttttctaaac
    47281 gatctaattc ggtaaataac cgtcgattag gtaaaccgct gtgagcagag atcactgaat
    47341 gcgtcgaatt tccaccaagc gggaaagagg ttccttgcaa gacgcctgct ccattttcaa
    47401 ggatcctacc attgagccga tcataaagtg aaatatttaa ttggatctta ggtatattga
    47461 tacttccgat cagccattcc tctaattggc tggatgttgt actttcattt ctgttctttt
    47521 cattgaaagg atcagtacca ggattgaatc cttggtcagt taatcgttgg tttttctcct
    47581 ccattttctt tcgctgcact tcaatttcag ctgcggtccg tttttgtact tgttcgatac
    47641 gtttttgatc aataaaacta tttaaagaat ccacataaaa tggatataaa gcgatcagta
    47701 accctacgac gatacatatt aataaaaaca aacgaaacaa aagtcgacga gactgttttt
    47761 gcttactctg cttcacatgt gtcctccctt gcaagttttt ttgttctttc ttttattata
    47821 tgggttcagg aaaaaaatag aaaatgaaag ccgatgaggt tgtgaaaaag ctgtcctgca
    47881 tcaagtaata agtacagccc aaaaaaatag ctactcatat tttttgttgg gttgggctta
    47941 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa actgcgttat
    48001 gctttgagta ataagagcgc tagtcagaaa atagcttctc atattttcta ggctagtgag
    48061 cttattaccg agaagcagct gattgaacac cgtttaccga gaggtcatga aaaagctact
    48121 ttgtttcgag tattaagaaa aattctgttt caaaacaagg agatgcaaca aaacttatgt
    48181 cgcatcctct ctccttagaa ctttcactta tgtttttctt gtaaaattca taaatatttt
    48241 catgaatttt ctaatttctt gcctacctct cgcataggat atatgatgaa tttatctaaa
    48301 gtttgcaaag gagttcgaca attgaaaaaa tatggcccat ttttagcaat ttttttcatt
    48361 gctttggcat ttctcatgcc tcatttattc acacataaac taattttggg tgctgatgcc
    48421 ccattccatt acaatcggtt ctatgatgcc gcgcaacaaa ttaaatatgg ggatttccat
    48481 tattttcctt ccctttatgg attccagcaa agtggcagga tcgtgaatgc cgtttacggc
    48541 ccatttttcg cttatttaca aggattgatc gtgttgcttg caggtagctg gtttcattat
    48601 cagattcttt caaatttcat tctttatctt atttcaggat tttctatgct gattttactg
    48661 agatatgtca atgttcgaaa ttgggtaagt gtaccagtag ccgttttatt catgagtacg
    48721 tattctattc aatactggtc tatcaaccaa ggattcacta gctgggggac ttgtttttat
    48781 cctttatgta tgatccctat tgctgatttc gtccttcata aaaagttccc tgttatcaaa
    48841 gtcgctgtct cagtgggact tatgacacag atccatttac tgtcttcgat catgttagct
    48901 ttgatgtata tcccttttta tctttattac ttttttaacc agactgaaaa aggaaaagca
    48961 ctgcttcaac tgattaagtc ggtcttgctt tattttgttc tgacagcaaa tatttgggca
    49021 ggattagcta ttgtttacag tgggaataag attcttcctc cctttgttaa cagagaaatg
    49081 agtcaaaagg cgatagatct ggatggcagt tattggctcc attaccctaa ggcctttcct
    49141 atattgatat tatcaggttt tttgcttttt attttctttc aacaaaagaa caccttattc
    49201 caaaaaatcg tgttgtacac aagtcttgtt ttcttttttc tttctactag cttgattcca
    49261 tggacgacat tgatcgaaaa aaatgtgcct tttattagtt tgatccagtt tccatttcga
    49321 tttttcgtgc catttacgct attgcttcta ctttaccttg ccctttcact gaaccagtgg
    49381 tctgaacaaa agtggttccg tcttttaagt atatttggca ttcttatttt ttcagcacag
    49441 acgatccaga acttatacca gcatctggaa aagtgggaaa atgaaacatt tgtcagtcga
    49501 catacttatc tattcgatac gccggaagaa gcaaggaaaa cattcttcag caaagatatg
    49561 tctaccagct tgttcgtttt ccaaaaaacc acacctgact atctgccaat ctataaggag
    49621 acaaaagaca ataaatacga ccggtataac gaagaaatac tagaaaacga aacaactttt
    49681 ataaaaacac atccaggcgg tcttttgaca atcaagtgga aaaacactgg ggacaaaact
    49741 gttcatatcc ctgttatcgt ctacgatcgg actgtgctac agcaaaatgg aaaaacattg
    49801 actgattatg aagtaacaga tatcgggacc ccaatcgtga agcagcaaaa aggaatcaac
    49861 gagctcacgc ttcattatca aacgccaatc tatttctatt tcattctttc tcttactttg
    49921 attggctggt ttaccttgct atgtttattt atttatcata gatacaagct tttacgtgct
    49981 taggcaaaag ggataaatac atccgatcaa aaaactctcc aaacggtata atcgtttgga
    50041 gagttttatt ttaatcagtt tttttatgtt atatgttata ttttttattc gttcacatct
    50101 aattcgattt ccaccccgta gtgatcactg atgattggac tatcgatccc atcaaaaatc
    50161 cgttgatatt ttttaacctg cattccttca gggacgaaaa taaaatctat acgcaatttt
    50221 tctgtatttt gttcccaacc atctattttc ttttccacag tagctgcttt tcctttttca
    50281 ttcgcgacaa catacgaatc ttgaagaaga aggcaacttt ctcttacagt ataataacct
    50341 tgagagtcta ctggattatt gaaatcacct aggaaaaaaa gtggtacgcg agtttccaaa
    50401 aagtagtttt ccaagttttt ccactcaaaa taaaagccac tttttttatc tgtccaccaa
    50461 gaaaagtggc agctcgagac aaaaatgtcc tgatcatcta tctttgtttt cccaatcaag
    50521 atcttgcgtg tctgataatc ttctttcttt tgaaattggg agacggtaag gacctgactt
    50581 tccaatgttt ttttcgtaag cagtgcattt ccttcttcat atttatcata accgatgtgg
    50641 ctcatttccc aactccaaaa atactctttt ccgtgttcct ttaaaagctg aactaaacgg
    50701 taggcaaaat tatctttacg gataggtact tgattcttta ccggacagaa cttcattagc
    50761 ttcgcaggtt ctacttcttc agattccagt aattggttga tttcttgtaa agcaatcact
    50821 tcataatctt ctaacaggat cttatctgct aattcttgca attttttctc tggttctggc
    50881 tcaagccagc tatgcgtgtt gagtgtcatg aatttcattg gttttctcct tgtcttcatt
    50941 tcgaaacaaa agggttgtga caaaattttt gtcacaacct ctccagcaaa tagtgttctc
    51001 ctgctaactc ctcagtaata agctcaatgg accaggagct attttttgtt tggctgtttt
    51061 tatcacttgg tgcagggcag ctttttcacg acctctccac taaacagtat tcaaactgct
    51121 gcttctcggt aataagccca accaaacgaa aaataggagg agctattttt gtttggctgt
    51181 tcttatgact tgatgcagga cagctttttc acaacctctc cttacatttt aatggagccg
    51241 atcactttac cagctgtcac tgtacctaat tgcttgatca ctacttcttc tactttatcg
    51301 tttgtaaata cgatcatgat cgttggatct ttttcttcgt tttggatcac atcaagatcc
    51361 atctcagcta atttcgttcc tgttcctact tcttggcctt ctttgacagc aatctcaaaa
    51421 gctggctgat tcatttggac tgtatcgatt cccatatgga tcaaaacttc gataccatct
    51481 ttcgtttcca gcccaattgc atgtttgcta gggaaaatac ttgtcacttt tccagcgatc
    51541 ggcgcaacaa cttctcttga tgttggaata acagcaaagc cgtcacccat cattttttgt
    51601 gagaataccg gatcttttac ttcagataaa ggaatcactt ctccagaagc tgcggaaact
    51661 agttcttgtt ctatacctaa gtattgctta ctacctgctt gtccatttcc tgtagaaacc
    51721 atttctggct ctggaatctc tgctcctgat tgcagcagat cttcgatatc tgatttcaag
    51781 acatctgctt ttggtccata aactgcttgg actccattgt ctttgacgat caatcccatc
    51841 gcccctgctc gtttccaggc ttcttcagtc cctacttttt ctcgatcatt tacacttacg
    51901 cgtaaccgag tcatacaagc atcgacatct ttgatatttt cttttcctcc cagcaaatga
    51961 atgatctgga ccacttgctg atctgctgtt cctgttccgc tgttagatac aacggtttct
    52021 tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag caaagttgaa ttttttgatc
    52081 aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga tcacacacaa cacgaagttg
    52141 ataagatctc cacctagacc cgctttgata gctaacggtg tccgagtcag caattcgata
    52201 ttcccaaatg aatggacccg caatggcaag atatctgcca tcgcaaatgc agccccttgg
    52261 atcactgaat aaatgacata taatggcaca gcagcaaaca taaacatgaa ctccaatggt
    52321 tctgttacac ctgttagaaa gacagccaac gctgctgaaa aatacattga tttatatctt
    52381 gctttcttat caggatcgac attacgatac atcgcaaaag ccattcccat caagatacct
    52441 gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg tccagttatg caacacaaac
    52501 tcatattgcg acatatcccc tgcaccttta agattcacta aatcagtggc ccaagccaac
    52561 cataacggat cttgcccaaa tacttgtgtc cccgcctgtg cgcctgataa aatctcatac
    52621 gatccgccaa gctgtgtata gttgatagga atcgtcagca tatgatgcaa tccaaatggc
    52681 aatagtaaac gttctaatgt tccaaataaa aacggtgcta gaatcggtgc gctttcttgt
    52741 gattctgcaa tccataaacc aaaattattg attccagcct gtacgtttgg ccacaccaca
    52801 gcaagaatca atgccacgat tgttgaccat aagataacaa caaacggaac aaagcgcttt
    52861 ccattgaaaa atgacaaagc atccggcaat tttctataat tatagtactt attataagcc
    52921 attgccccga caaatccagc gatgatccct acgaatactc ccatattcaa agctggtgct
    52981 tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg taccgaacag agtgtgggta
    53041 aatgcatctt cattcgcaag catgtcactt gtgacaccaa agatagcccc agtgatccga
    53101 ttgattaaaa taaatgagat cccagctgca aatgctccgc ctgctctttc ttttgcccaa
    53161 cttcctccta ttgctaaagc aaataaaata tgaagattac caataattgc ccaaccaata
    53221 ttttcaatta ctccgcccgt tgtgacaagc ggcgtccaat caggattgat caagggaatt
    53281 gatttcccta tactgatcat caacccagct gccggcataa ctgctaccac aaccattaaa
    53341 gcctttccaa atttttgcca aaactcaaaa ctaaatatct ttttcattcc gtttagctcc
    53401 tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt tttctttttt gtaaatgctt
    53461 tattttttat ttttctgtct ataccattct ataaaaccta taatacgtgt gttcccttct
    53521 ataattttct cctaaaaatt tattttttta agcttttttc tatgaaatat tttcttttgt
    53581 ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta taaaacagct cattaggagg
    53641 agaaaaatga aacagatcaa acgactattt gctattgatc catggaaaat cagcacgaca
    53701 caattagata aaaaaaatct tcgactgcaa gaatctttaa caagtattgg aaacggttat
    53761 atggggatgc gcgggaactt tgaagaaact tactcagggg accaccatca aggtacttat
    53821 ttggcgggtg tctggtatcc cgataagaca cgtgtcggct ggtggaaaaa tggttatcca
    53881 gaatactttg gaaaagtgat caacgccttg aactttatcg cgatagatat ttcgatcaac
    53941 gatcagctga tcgacttggc ttctttggaa ccagaagact tttactggga attagatatg
    54001 aaaaatggtg tattatctcg tacattcaca atcactactt ctactaataa agtacgcatt
    54061 tccttcgaac gttttctgag cattgtcaaa aaagaatcag cttatatcta tttaactata
    54121 gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa aattggatgg aaatgttcaa
    54181 aacgaagaca gcaactatga tgaacatttt tggaatgaga ttgatcgtgg aactatagaa
    54241 gaaatcggct atttgacgac taaaactatt ccaaacaact ttgggattga acaatttact
    54301 gttactgcag caatgcgcca tttcgttaat ggaaaatcag tcatccctga ctatcacgaa
    54361 gatactttag cattacatgg ttgcttgtct gcagaattat ctcaaggtga gacatggtca
    54421 gtggaaaaag aaattatcgt tcttactagt agagatattc cagaagcgca gcaagcagaa
    54481 aaagctgtct cagaattaac gaaccagaca aacaattata tccaagcaaa agaagagcaa
    54541 acagcggctt gggcagaacg atggaagctt gccgatgtgg tcatcgaagg agacgaagaa
    54601 gcacaacaag gtattcgatt caatttgttc caactattct ctacttacta cggcgaagat
    54661 gaacgattga atatcggccc caaaggattc acaggagaaa aatacggcgg agccacttac
    54721 tgggatacag aagcgtatgc tgtccctctt tatcttgctt tagccaaacc tgaagtaact
    54781 aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag caatccacaa cgctcagcaa
    54841 caaggattaa aaggcgcgct ttatcctatg gtcaccttca ctggtgtcga atgccataac
    54901 gagtgggaaa tcacattcga ggagattcac cgaaatggcg cgattgccta tgcaatctac
    54961 aattatacga attacaccgg cgatacggaa tatttgaaaa atgaaggact ggaagtttta
    55021 gcagaaatcg cacgcttctg ggcagaccgt gtccattttt caaaacgagc aaataaatac
    55081 atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca atatcaataa caattggtat
    55141 acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag aaaattattt aacttatcaa
    55201 aaagaagctg ctatcactat cagcgaagaa gaactagcca aatggaaaga tatcgcagag
    55261 aacatgtact tcccagttga cgaagaatta ggaatctttg tccagcatga cacattctta
    55321 gataaagatc tcatgcctgt atccgatatt cccacaagcg atcttcctct gaatcagcat
    55381 tggtcttggg acaaaatcct tagatcttgc ttcatcaagc aggcagatgt actacagggg
    55441 atttatttct tcaatgatac ttttacaaaa gaagaaaagc aaaaaaactt tgatttctat
    55501 gaaccaatga ctgtccatga gtcttccctt tcgccaagta tccattcgat tttagcagca
    55561 gaattaggaa tggaaagcaa agctgtagaa atgtaccagc gaacagctcg cttggatttg
    55621 gataattaca ataacgacac agaagacggc ttgcacatca cttccatgac tggtagctgg
    55681 ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc atgatcagtt gagattcgca
    55741 ccatttctgc caaaaacttg gaaagcttat tctttccata tcaattatcg cggacgttta
    55801 ttgttcatct cagtcacaga tgaacaagtt actttcgagt tattagaagg ttctcctcta
    55861 gaaatttacg tctatgaaca gtcttattct ttagcggatc gtctagaaat aacgctaaga
    55921 aaggaggaag caaatgttta aaggggtatt gtttgattta gacggtgtca ttaccgatac
    55981 tgctgaattc cattatcgtg cttggcgaaa gcttggtcag gaaatcggca ttacgatcga
    56041 ccacacattc aacgaacagc taaaaggcgt cagccgagaa gactcactct ctttgctttt
    56101 agcttatggc ggaaaagaac actctttttc aaaggaagaa tttgcagaat tagcgaaaag
    56161 aaaaaacgat tattatttag agatgatcca gacgatcgaa ccaaaagatg tcttccccgg
    56221 ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc aagattgctc ttgcttctgc
    56281 aagtaaaaac gggccattcc tacttgaaaa aatgggtctt acaccttact ttgatgctat
    56341 tgccgacccg gcagaagcaa caaacggaaa acctgcgcca gatatcttcc tccttgcagc
    56401 aaaagctgtc ggactaaaag cagaagaatg tatcgggatc gaagacgcgc aagccggtat
    56461 ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg ggacgtgctg aagaccttgg
    56521 agaggagatc ccgcttgtcc ctgatacgac ttttcttacg atcgattact tgaaagaaaa
    56581 gtggcatgac catggataat ccagagatct ccgtcgtacc agttccagag actgaaataa
    56641 agaaaatcac attgaagaac gactacttag aagtcgttct tctcaattac ggtgcccgcc
    56701 tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga aaatgtttta ttgtcttacg
    56761 atcgtttcga agatgttcta acagacaaaa gcttttttgg agcaactgtc ggacctgtag
    56821 ccggaaggat ccgggatgct tcttggggtg agcatagatt ggagaaaaac tgcggatcac
    56881 accatatcca cggtggcacg aaaggctggt ctttccaatt ttgggaagta gaagttttca
    56941 aaactccatc atcaataggt gtggtctttt acttaaaaga tgaattttct acctatccag
    57001 gaccaatcac tgctacgatc acttaccgtt taacaaaaaa cgaattagag atgacaacct
    57061 ctgctagtag ccttgttgaa accatctgca atccgaccaa ccatgcctac ttcaaccttt
    57121 caggaaatgg aacaagagac atctatgaac atcagttaac ggtcttttta gatggtgtgc
    57181 ttgaattaga tcaagaaaaa ctgcctactg gaaattggac aaaaaaagaa aaactgccaa
    57241 tcgactttag aaaatcccca acactgcaag agatccttgc atgttatcca gatggactag
    57301 atgatgtatt tcttttgcat catcctaggc tttccaaaac ttccttacaa ttattcgaga
    57361 agcattctgg tcgccaaatg actatagcaa cttctaataa aagtatggta ttattttcta
    57421 cgactggatt cgaagctgat ttctctgtca atggaaaaca gatgcattca aactacggat
    57481 tagcaatcga accgcaagaa atccctgata tcgttcattt ccccaaattt ggatcgatca
    57541 acctccatcc tggtcaagaa cggatcagtc agacgattta tcggttctct gcacaataac
    57601 aagagaaagg tgtttttatc atggctatta ccgtaaaaga tgtggcgaaa aaagccggtg
    57661 ttgccacctc gaccgtctct cgtgtcatca acgatcatcc tagtatttcc gagacaacaa
    57721 agaagaaagt ccgaaaaatt atggatgaac taggttatgt tcctaacatg actgcccgca
    57781 atctaggaaa acgcatttcc agtgccatcg gcgtaatctt accccctctt gattcaaaag
    57841 agcggatcgg taacccattt tatcttgaga tcatggaagc aatcaacgaa gaagcgcgga
    57901 attatgatat gactacagca atcgctactg ctaaaaattt tgaattgctg ttagagaacg
    57961 tccagcgtat gcatttacaa aaacaagtag atggttttat tctagtgtat tcagatagtc
    58021 aagatactgt cattgattat ttataccaaa accacatccc tttcactttg atcggacagc
    58081 cttatcatca tgagaacgaa atcgtttatg tagataatga taaccagctt ctaggtaaac
    58141 aagcaacaga atttttgatc cagaatggac ataagaatat cctgtttgtg acaaacacga
    58201 ctcatgaaag tttgtatttc gaacgatatt tcggttatca aaaagcaatg atgatggctg
    58261 gtcttcctgc gttatccagt gtagtactag agcatccgga ggattatctg tcctttgaga
    58321 aaacgttgaa agaaaacgaa gccacagcat tggtggtcat tgacgatatc ttcgcccttc
    58381 gcacgatgca gttggcgcaa atgtatggct atgtcattcc agagacactc tccgtcatta
    58441 gcttcaacaa ctcgatcttc tcaacattaa cacaccctta ccttacaagt atcgatatcg
    58501 atatctccga attaggacgt gtcgctatgc aaaagctgcg cgaattgatc catgaagaat
    58561 tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat caaaagagag acagtcgttc
    58621 ctttagaaaa cccatactaa atagctgact cactaaatac gctgaccatt tatcctatgg
    58681 tcagcttttt atgtaaaaga agatactact tctaacacat tgtttatctg taaaaaagag
    58741 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa
    58801 gcagaagcaa ctccttcgga aataagctga aattcacaaa aatttaaaaa gcaattttct
    58861 tgagtttctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttccttcct
    58921 tcaatttttt acacgtcgac gattcaataa aaaaccgcct aatattgtaa agacaggtgc
    58981 aagaatcgag aaaaaggcga atggcaagta atgcaacgcg ctaactttca tcgttcccat
    59041 aatgaatgtg ccgctgactc cccacggtac gagagaattg acggtcgcac ctgaatccgc
    59101 tagtgttcgt gtcaaatagg tttttgataa gccaacgtca tcaaaagaac gcttgaatgt
    59161 ttctcctgga agaatgatgg acagatactg ttctccaacg atcagattga ttccgataca
    59221 gctcaaggca gtaaacccga ctaaacgaga aggtcggtcc atcttttctt ttaattcttg
    59281 aacgatggta tcaacgatca gatatttgat cagtaaccca cctaatgcca atgcaagtaa
    59341 aatcaaacta gcagaaccta acatactcat gatcccgcct cgagaaagca gttcatctag
    59401 ctttggatca ggtgtatttg cgacataacc attcatcaag atgtctgctg cttttccgac
    59461 cgtcaaactt ttctcatgga taaaagaaag aacgagagca gctgtcgatc ccgctagtaa
    59521 tgtagggata gccggaattt ttttgattgc ccccccaaat aaaataacta gtggaatcaa
    59581 tgtccaagga gagatccaaa agctcgcttt catgttttgg atcatttcat tgatactagc
    59641 tagatcggct gaatgactgt tatgtcctaa tatcgtataa aaaatcaacg aaaggacgaa
    59701 tgctgggaaa tccgtataac gcataccagc gatatgttcg aatagattaa ctcctccaat
    59761 acccgctgcc aagttggtcg tatctgataa aggcgagcag ttattcccta aaaaggcacc
    59821 ggaaacgact gcgcctgtag tcattgcatc attgaatcca agtaaatgac cgatccccaa
    59881 aaaagcaatt ccaatcgtcg aaatcgttgt aaatgaactg ccgacagtca ctccgacaat
    59941 tgcacaaaca agaaatgctg tcggtaaaaa gaaacgcaca gaaacaagtt taaatccata
    60001 aaccataatc gtagggatgg ttccagataa aatccatgaa gcaactaaga caccaatcaa
    60061 aagaaaaatc aggattggaa tgatccctgg agtgattcct ttgatgatcc cgtcatggat
    60121 ctcatcccat gtgaactttt taaatctgcc ataaaacaac aaacacatga aagctgcaag
    60181 gatcgggata tgtggcgaga gctggaatcc gatgatcagt actcctagga gtgccaacaa
    60241 cacgatcaat acagcgatac tttcttttaa agaaatattt ttattcatcg tcgatctcct
    60301 atttcaaatg aaaaggccct gccatgaaaa aactattcac ttggcaggac ctcctactaa
    60361 atcagcgaca aaacgtcggc tgaaagactt atttttttat ttttcttcta aacttttttc
    60421 tacctcaatc ccccaagaat ctttcttgaa gtgattgatg atcaatggaa taacgaacat
    60481 gaccactgtg atagctgcta ctaataatgc atcactcaat gtcgctgtac ctaatgtagc
    60541 aaatacgaca acgatgatag agaagatcag catgcagctg actacccaag ctaatccgtt
    60601 tccgttttta ccaattcggt aaggacgttc gaattctgga tgtttcttac gcattttaag
    60661 caatgaaaca gcaatcaaaa tgtacacgat acaataaata acagttgtag catttgtaag
    60721 tgtcaagaat acagcactta cgtcatccat tacaccataa agcaaagcaa ataatgaaat
    60781 aacgattgat tgtgttagaa cgacatttct agatacgccg tatttgtttt ctttatggaa
    60841 tccgaatttt ggtggaagga atccttcacg agctacttgg atgattgttt ttgatggtcc
    60901 agtaacccaa gctgataatt gtaaaagcac accaataaat accatgaagc tgaagatatt
    60961 tccgatcact tctggtaagc ctaggatttg acagtcaatc aagataggct gcgtgatatt
    61021 tgctaattcc atttttccat ctggtacgac atttgctaca agcatcgcat tgatgacatt
    61081 caataaaact aatccgatca aagcgataaa tacgcctttt gtataatttt ttgttgcgtc
    61141 tttcaaacga ggcatgtaaa cagaactcat ttcgatccca acaaaaataa atgtgatacc
    61201 agctaagtat ttcaatgaat caatatgttc caagtcaggg aaagcttttg accatgaaaa
    61261 gtcacctaga tagccgtttg agactagtcc gactttgaag gctgccaaaa cacctaaaac
    61321 gaacatgatg actactggga tatatacccc tagccataca ccgatgttcc cgccgacttt
    61381 tgccatatcg aatttcaagt tcaagatcgt gatgatccag tagataacta aaatacaacc
    61441 taaaacgaac cagtgattat tgccaagttc tacattacca aatgtgtttc ctaacaatgg
    61501 tcctaaagta gaagcgacca ttaccatacc tgggaacatt tgcacccata atagccatgc
    61561 aacgacaaat ccccatttgc tgcccagtgc tgtttttacc cataactgag gaccgccttc
    61621 ttgaggaagc atcgtggaaa gctcaccgga aaccattgat atcggaccgg caaaaaacaa
    61681 aactgcaaaa attgaataaa aaatcaatgt ccaaccgact gcggctaacg taggaatact
    61741 ccgaaccgtt gcacacagtg ccattgtcag accgataaag gtcccaagac ccattttact
    61801 tacttcagat ttactcaccg ttgacactct ccttgatttt ttggataaaa taacggtgtc
    61861 tttgaagtag tttcagcaat aaacgaaaca attcagcgaa tatttgaggg gattctttga
    61921 gtttattgct tgaagggttt cttcaaagac aacctcaaaa caaagggatc gaagagctta
    61981 ggtctcccct tgctcttcag atccaaacat gcgtcagaaa cagttagtat tcaagctgtt
    62041 ttctaacatg attaccttgc ttattttgct tcgcttgcca ataattggtc tgcgtagatt
    62101 ttttccaatt tttcttgaag agctgctttg attttttcag catattcttc aaagtgttct
    62161 tctttgttca tgtatggtgt cattacgctg gcacgcaata cagttacttt tccggcacgg
    62221 ttccattctt catctgagaa tcctagttgg ttcacaaatt gtaatgggct gttgccatag
    62281 tctgggatag caaagtcagt atgtgaagtt aagaactcat taccgtaaat gcttcctttc
    62341 acgtatgaag aataatcata tacgtcatgg ttcaatttat tcattgcaac taagtcatcg
    62401 ttgccttttt ctttgaatac atagtctacc atattgaaat ctggataagt taatggatga
    62461 acttcgattt ctttgtctcc tactttaaat gacaagtcat tcaagaagtt gtagaaacga
    62521 tgtgcacctt cgattgatgc acccattaat ttaccatatc ctgtgacatt caaaggtaag
    62581 acatggtgtg ctgcccaaac gctggctgct gttgcccctg cttttgaacc ttccaagata
    62641 taagccccaa gtaatgctgg gatatctgcg cctttttcaa atacatatgt cgcaaagtaa
    62701 gagataacgt ctctcatgcg gatatctttg atgacgatac caccggcaga atatggaaca
    62761 tagcccattt tatgtgggtc aatcgttaca gattctgctt cttcgattgc tttatatgca
    62821 ctgtgtactt cttctaagat ataatctttg ttttctgtaa agacattgta tttgtaatga
    62881 acatctttta aatcttcaaa tggaatgaag ttgttgtctt cgtctaagaa aatcgcacgg
    62941 ccgtatccgc cataagcagc atctacgtgt aagtagaagt aaataccgtc tttttctaat
    63001 acgcgacgca atgcaacgat tttatcgata ccgtcgattg ctccttcttc tgttgatcct
    63061 acaacaccga ctacacctag gattggtgtt ttttcagctg ctagaccacg aacgattttt
    63121 tcaagttcgt tgatgtccat gcgatagttg tggtcaactg gcactggaat cacttggtct
    63181 aaaccgatac cgatgatgtc ggcagctttt agccatgaat agtgtttagt ttgtggaacc
    63241 aaccattttc ctaatttttc taaatgtttt ccgctacgtg ctgagtgagc tttgatttcg
    63301 tcgatttttt ctgggacgct gtctaacaga ttcatgattt cttctgttga caagttcatt
    63361 aattcccaat cgctttttcc agcaactagt tcaggtgtca cttctttcat tgcaagcggc
    63421 aatgatttga tgttgcgggc ataccaaagt ccttctaagt ttgctaaaga accatcagca
    63481 acgatgtggc cccagccatc tttatagctc attaattttg caaattccat tcctacttct
    63541 tcttccattt ggcttgttgc aggagaagat tcataggcaa cgttattacc attccatagc
    63601 atagcaaaat tgtacgctag taaagatggc atcaatgttt cagagttcat atgaccccag
    63661 taacgtccgg cattatgcca aggaacggaa tgggtacgca tccgcgctga aatttctgat
    63721 agaacatcct ttgttttatt cacagtgtgt tcaaaactag cactgctttt ttcttctggc
    63781 gtaataatag gcatgtcttg aggcatgtag ttttgacgcc agcctaaatg ttcatccacc
    63841 aattcattca acaaagcttt atagatttgg ccgttctcag ctttgtcccc aatgaatagg
    63901 gcatttaggt ttagatcttt cgacaatgat tcactcatag tgaaaccctc ctaaaaaaat
    63961 atttaaattt tgtcgagccc tacattacac ctgttcttct attctgtaaa ctaagttttg
    64021 gcctgatttt taatcatatt ttaatatgta tcaattatcc gaattgttca cacttttgaa
    64081 ataattaaaa aaaacgaggg aatttacaaa tgtaaactcc ctcgcaaaga ttgtattaac
    64141 gacaaccagc gttctatttt aggatgggtt tcaaaattgg aatgttgtaa tagtatttcc
    64201 ctttttcaag gtaaaaactt actaaattct aatcaattac tttggccaaa aagtaatttt
    64261 tcttaccttt tcgaataacc acaaacttac catcgaattc atcggatgga tttatgacaa
    64321 aatttaaatc ggtaacgcgg tcaccgttaa tacttatagc tccgtttgaa acatcttcac
    64381 gtgcttgacg tttagatggt tcaatttttg tggaaacaag cagttccacg atattttcag
    64441 gtgtgcttga aatttcaacg ttcggcattt taccgaatcc ttgcgcgatt tcttccgcat
    64501 ttagttcttt aatatttcca gagaacaatg cttgcgttat tttttgcgct tctttcaagt
    64561 cttcttcact atgcacaaat cttgtaactt cttctgccaa acggcgttgt gcttcacgtt
    64621 tctcaggttc tgtctctact tttttcgcta agtcttcgat ttcttcttga gacaagaaag
    64681 taaagaattt caagtatttc actacatcgc gatcatcttg attcaaccag aattggtaga
    64741 actcaaatgg tgaagttttc ttcggatcaa gccaaacggc accgcctgca gtttttccga
    64801 atttcgttcc gtcagccttc agcatcaatg ggatcgttaa accgaacgct tctgcttctg
    64861 gtccttctaa ttttcggatt aggtctaatc cagctgtgat atttccccat tggtctgctc
    64921 caccgatttg caattgcaca tcgtatgttt tatgcagatg caaaaagtcg atagattgca
    64981 ggatttggta agtaaactct gtaaatgaga ttcctacttc caaacggctg gcaacgatat
    65041 cttttgctaa catcgtgttg atgttgaagt ttttaccgta gtctctcaaa aattcaagta
    65101 aagagatttt tgacaaccag tcgtagttgt tcacaaatgt gatatttgca tctttaccaa
    65161 acaattttct catttgattg gaaagtgcgt ccacgttatg ttgcacttgt tccatcgttt
    65221 gcaacacacg ttctgttttg cggccgcttg ggtcgccgat cgttcctgtt ccgccgccaa
    65281 tcagaatata tggatgatgg cctgccaatt ggaatctttt catcatcata aatggaatca
    65341 aatgtccgat atgcatcgaa tcaccagttg gatcaacgcc gcaataaagt gaaatgctct
    65401 tttcttcaac gagctcgcga agtctttctt cgtttgtttg ctgattaatg gcgtcgcgcc
    65461 aagttaattc atcaataata ttcattttac tgacctccgt gtagtttttt tatgaaagca
    65521 ggaacctgct agcatatgta attaattatt tcttcaggat aggataggaa ataaaaaaag
    65581 tcccttggca tataagccaa gggacgaaat ctcgcggtac cacccaattt gtatgtctag
    65641 atatccaaca tacctctcga acatttaacg ctgtcgcgtg gcaatcactg ccaaagactc
    65701 ctgaaatgta attcgcttta aagcatgtac tggtttgcag cgaccaccag ctttctgaaa
    65761 cattggcttt aacaactact tcgtttcgat cttcgtcttg ttattattaa tatgtgtcta
    65821 tcatagagcg caactttttc tttgtcaaca gaaaaaggat atttttttta aattcctcat
    65881 tttttctttt ttgaatccgc tctaattccg aacattttat tttcaattat tgttaaaaga
    65941 aataactttt tctgtatttt ttctgcaatt tatcgagaag tcactttact ttgcctctta
    66001 ttctttcttc gttacaataa ggaatgggac aagtaaaaaa atgaaaaagc aagggacgag
    66061 gtatatggaa aaaaagcaaa aaattatttt aggagccact tctgctttaa ctatcgttat
    66121 tttaattcta agtttgtttt ttgtcatccg atcatctaca aaaaaagaac caatcattcc
    66181 tgaaaaaaaa ataacagaaa aagcaaaaga accgacagat gataagcaac aactagttac
    66241 tcaagcaaaa aatctggctc aaagctacga ttatgaccaa gcgattgcgt tattggaaaa
    66301 agatagtaag aacggatcta ctgaaacaca acagcttctt caaacactaa aaaaggagaa
    66361 agaacaactg atcgactggt ctgatcctac tcagatctct catgtctttt tccacagctt
    66421 gatcgtggac ccgcaaaaag ccttccactc tcaacaggca caaggataca aagattatat
    66481 ggtgactgtt gaagaattca accgttcgat cgaacaactt tatcaaaatg attttgtttt
    66541 agttaatttg aatgatttga tccaaaaaga cgaacaagga aatctttcct ttaacgggct
    66601 tgcgctacct gaaggaaaaa aaccattggt tctttcacaa gatgatgtca gctattatga
    66661 atatatggat aattccggtt ttcctagccg attggtcgta tcaaataaca atgaaatcaa
    66721 aaatgtctat aaagagaaag ataaagaaga aatcggtgac tttgacatgg ttcctctgat
    66781 cgatgccttt gtcaagaaac atcctgattt ttcttatcga ggcgcaaaag gtaccttggc
    66841 cctgacaggt tacaacggag ttttaggtta tcgaacatca aaatctcaat atggagacaa
    66901 tgaaaaaaca aaaaaagaga ttaaagaagc taaaaaagtg gcggaacagt tgaagaaaga
    66961 cggctggagc tttgcttccc atacatgggg tcacttgaat atgactcaag cttcactaga
    67021 agatgtaaaa aaagacaatg aattgtggca gcaagaagtt gcacctatcc taggaaaaac
    67081 aaatattctg atttatccat ttggtgcaga catcagcgac tggcagcctt attcagcaga
    67141 aaatcaaaaa tttgcttact tgaagagtca aggtttcgat attttctgta atgtcgatgc
    67201 ttctacacca gcttggggac aattaggtac ggatttctat cgaaatgctc ggatcaatat
    67261 cgatggcatt cgttttcaat ctgatttaga cggaaaaaat cctattcttg atccattcat
    67321 caatgtccat gatgtctatg acaaagaagc acgcgaatct gcttgatttt acgtcattta
    67381 tgaaccaaaa aagccgaact gacattttat cgtcagtttc ggctttttta tgctttattt
    67441 cttcttcggt gcgattccct tgatcaggac ttctgtccat tctgctgtat atggaagcag
    67501 ttttgcgtat atctccggct tagaaattcc ttctaccatt gcttgaaaat agaggatcaa
    67561 gaactcatcc gaatatttca aatccacttt tccttctttc cgccctcgat ggaaaagatc
    67621 aagcatgata ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa attctttttt
    67681 ttcttttttt gtatagtatg tcatcaaatc gatataaaat tgtttgttca ctcgattgag
    67741 ataacggatt ttgttttcac tcatggcaat cagtgtttct tcaaatggaa gattttccgc
    67801 aatcaacgct ttcgcttctt gccctaaatc attcataaat gcaaaaaata cttcataaat
    67861 cagcccttcc ttgctgccaa aatatttaaa aatcgtcgct ttacttacct gagcgttctc
    67921 agctacctta tccattgtca ctccagcggt tcctatgtct gtgttcatca agtcaaaagc
    67981 tgcttgtaaa atctctttct ttttctcttg tgctctcttt tcaaaaccgt tcatttgtag
    68041 agcctcccgt aattttaaac ttttataata tatatagttc aaatttattc tgag
//
LOCUS       DANKBF010000006        62197 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000006 DANKBF010000000
VERSION     DANKBF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="PWY89_000421"
     CDS             complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="PWY89_000421"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL2065482.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(1390..3468)
                     /gene="topA"
                     /locus_tag="PWY89_000422"
     CDS             complement(1390..3468)
                     /gene="topA"
                     /locus_tag="PWY89_000422"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2065483.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="PWY89_000423"
     CDS             complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="PWY89_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2065484.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(4503..5270)
                     /locus_tag="PWY89_000424"
     CDS             complement(4503..5270)
                     /locus_tag="PWY89_000424"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2065485.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="PWY89_000425"
     CDS             complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="PWY89_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL2065486.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            6383..6781
                     /locus_tag="PWY89_000426"
     CDS             6383..6781
                     /locus_tag="PWY89_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2065487.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            7060..7560
                     /locus_tag="PWY89_000427"
     CDS             7060..7560
                     /locus_tag="PWY89_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2065488.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="PWY89_000428"
     CDS             complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="PWY89_000428"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2065489.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8346..8573)
                     /locus_tag="PWY89_000429"
     CDS             complement(8346..8573)
                     /locus_tag="PWY89_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL2065490.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="PWY89_000430"
     CDS             complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="PWY89_000430"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2065491.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(9108..9821)
                     /locus_tag="PWY89_000431"
     CDS             complement(9108..9821)
                     /locus_tag="PWY89_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL2065492.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(9824..10720)
                     /locus_tag="PWY89_000432"
     CDS             complement(9824..10720)
                     /locus_tag="PWY89_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2065493.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(10878..11720)
                     /locus_tag="PWY89_000433"
     CDS             complement(10878..11720)
                     /locus_tag="PWY89_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2065494.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11852..12505
                     /locus_tag="PWY89_000434"
     CDS             11852..12505
                     /locus_tag="PWY89_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL2065495.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(12568..13095)
                     /locus_tag="PWY89_000435"
     CDS             complement(12568..13095)
                     /locus_tag="PWY89_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2065496.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(13115..14062)
                     /locus_tag="PWY89_000436"
     CDS             complement(13115..14062)
                     /locus_tag="PWY89_000436"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2065497.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(14074..15960)
                     /locus_tag="PWY89_000437"
     CDS             complement(14074..15960)
                     /locus_tag="PWY89_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2065498.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(16120..18198)
                     /locus_tag="PWY89_000438"
     CDS             complement(16120..18198)
                     /locus_tag="PWY89_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2065499.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(18211..18486)
                     /locus_tag="PWY89_000439"
     CDS             complement(18211..18486)
                     /locus_tag="PWY89_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065500.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(18848..19303)
                     /locus_tag="PWY89_000440"
     CDS             complement(18848..19303)
                     /locus_tag="PWY89_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065501.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(19457..20665)
                     /locus_tag="PWY89_000441"
     CDS             complement(19457..20665)
                     /locus_tag="PWY89_000441"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL2065502.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(20777..21109)
                     /locus_tag="PWY89_000442"
     CDS             complement(20777..21109)
                     /locus_tag="PWY89_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2065503.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            21257..22132
                     /locus_tag="PWY89_000443"
     CDS             21257..22132
                     /locus_tag="PWY89_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2065504.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(22214..22795)
                     /locus_tag="PWY89_000444"
     CDS             complement(22214..22795)
                     /locus_tag="PWY89_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL2065505.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(22801..24060)
                     /locus_tag="PWY89_000445"
     CDS             complement(22801..24060)
                     /locus_tag="PWY89_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2065506.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(24062..25108)
                     /locus_tag="PWY89_000446"
     CDS             complement(24062..25108)
                     /locus_tag="PWY89_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065507.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(25343..25618)
                     /locus_tag="PWY89_000447"
     CDS             complement(25343..25618)
                     /locus_tag="PWY89_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2065508.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(25861..27171)
                     /gene="der"
                     /locus_tag="PWY89_000448"
     CDS             complement(25861..27171)
                     /gene="der"
                     /locus_tag="PWY89_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2065509.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(27353..28582)
                     /gene="rpsA"
                     /locus_tag="PWY89_000449"
     CDS             complement(27353..28582)
                     /gene="rpsA"
                     /locus_tag="PWY89_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2065510.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(28717..29397)
                     /locus_tag="PWY89_000450"
     CDS             complement(28717..29397)
                     /locus_tag="PWY89_000450"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2065511.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(29446..30081)
                     /locus_tag="PWY89_000451"
     CDS             complement(29446..30081)
                     /locus_tag="PWY89_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2065512.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(30147..31550)
                     /locus_tag="PWY89_000452"
     CDS             complement(30147..31550)
                     /locus_tag="PWY89_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL2065513.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(31537..32568)
                     /locus_tag="PWY89_000453"
     CDS             complement(31537..32568)
                     /locus_tag="PWY89_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065514.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            32599..32820
                     /locus_tag="PWY89_000454"
     CDS             32599..32820
                     /locus_tag="PWY89_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL2065515.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(32985..33722)
                     /locus_tag="PWY89_000455"
     CDS             complement(32985..33722)
                     /locus_tag="PWY89_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL2065516.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(33875..35215)
                     /locus_tag="PWY89_000456"
     CDS             complement(33875..35215)
                     /locus_tag="PWY89_000456"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL2065517.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(35452..36975)
                     /locus_tag="PWY89_000457"
     CDS             complement(35452..36975)
                     /locus_tag="PWY89_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL2065518.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(37353..37964)
                     /locus_tag="PWY89_000458"
     CDS             complement(37353..37964)
                     /locus_tag="PWY89_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2065519.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(38087..38212)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(38294..39010)
                     /locus_tag="PWY89_000459"
     CDS             complement(38294..39010)
                     /locus_tag="PWY89_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2065520.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(39016..39585)
                     /gene="scpB"
                     /locus_tag="PWY89_000460"
     CDS             complement(39016..39585)
                     /gene="scpB"
                     /locus_tag="PWY89_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL2065521.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(39569..40366)
                     /locus_tag="PWY89_000461"
     CDS             complement(39569..40366)
                     /locus_tag="PWY89_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL2065522.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(40336..40707)
                     /locus_tag="PWY89_000462"
     CDS             complement(40336..40707)
                     /locus_tag="PWY89_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2065523.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(40726..41613)
                     /gene="xerD"
                     /locus_tag="PWY89_000463"
     CDS             complement(40726..41613)
                     /gene="xerD"
                     /locus_tag="PWY89_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2065524.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(41709..42182)
                     /locus_tag="PWY89_000464"
     CDS             complement(41709..42182)
                     /locus_tag="PWY89_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2065525.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(42287..43150)
                     /locus_tag="PWY89_000465"
     CDS             complement(42287..43150)
                     /locus_tag="PWY89_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2065526.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(43346..45445)
                     /locus_tag="PWY89_000466"
     CDS             complement(43346..45445)
                     /locus_tag="PWY89_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2065527.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(45665..46774)
                     /gene="rpoD"
                     /locus_tag="PWY89_000467"
     CDS             complement(45665..46774)
                     /gene="rpoD"
                     /locus_tag="PWY89_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2065528.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(46798..48639)
                     /locus_tag="PWY89_000468"
     CDS             complement(46798..48639)
                     /locus_tag="PWY89_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2065529.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(48821..51136)
                     /locus_tag="PWY89_000469"
     CDS             complement(48821..51136)
                     /locus_tag="PWY89_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2065530.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(51321..52181)
                     /locus_tag="PWY89_000470"
     CDS             complement(51321..52181)
                     /locus_tag="PWY89_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL2065531.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(52240..52761)
                     /locus_tag="PWY89_000471"
     CDS             complement(52240..52761)
                     /locus_tag="PWY89_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2065532.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            53039..53800
                     /locus_tag="PWY89_000472"
     CDS             53039..53800
                     /locus_tag="PWY89_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2065533.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            53826..56417
                     /gene="mprF"
                     /locus_tag="PWY89_000473"
     CDS             53826..56417
                     /gene="mprF"
                     /locus_tag="PWY89_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2065534.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNAQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(56605..57792)
                     /gene="tuf"
                     /locus_tag="PWY89_000474"
     CDS             complement(56605..57792)
                     /gene="tuf"
                     /locus_tag="PWY89_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2065535.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            58160..58516
                     /locus_tag="PWY89_000475"
     CDS             58160..58516
                     /locus_tag="PWY89_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2065536.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(58757..59761)
                     /locus_tag="PWY89_000476"
     CDS             complement(58757..59761)
                     /locus_tag="PWY89_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL2065537.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            59927..60778
                     /locus_tag="PWY89_000477"
     CDS             59927..60778
                     /locus_tag="PWY89_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2065538.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            60795..61040
                     /locus_tag="PWY89_000478"
     CDS             60795..61040
                     /locus_tag="PWY89_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065539.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            61227..61931
                     /locus_tag="PWY89_000479"
     CDS             61227..61931
                     /locus_tag="PWY89_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065540.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        17334 a        13010 c        10709 g        21144 t
ORIGIN      
        1 aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt
       61 gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt
      121 gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag
      181 ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt
      241 ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg
      301 ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc
      361 tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct
      421 ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct
      481 aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag
      541 cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa
      601 ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc
      661 gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct
      721 ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg
      781 aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac
      841 ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca
      901 tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa
      961 gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt
     1021 atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg
     1081 cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga agtgattact
     1141 accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt
     1201 cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc
     1261 gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa
     1321 gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc
     1381 tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt
     1441 accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac
     1501 tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct
     1561 gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc
     1621 gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa
     1681 cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc
     1741 tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt
     1801 ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat
     1861 tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa
     1921 ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt
     1981 caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg
     2041 ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg
     2101 ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag
     2161 ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg
     2221 catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc
     2281 taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga
     2341 ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg
     2401 tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt
     2461 tttgatcttt cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc
     2521 tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat
     2581 caagccgact gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat
     2641 catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt
     2701 agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa
     2761 gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt
     2821 tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt
     2881 accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat
     2941 aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg
     3001 ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg
     3061 tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc
     3121 ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta
     3181 cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt
     3241 ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga
     3301 aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc
     3361 tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt
     3421 cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac
     3481 tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca
     3541 tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa
     3601 attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat
     3661 gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa
     3721 gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac
     3781 atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg
     3841 ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca
     3901 agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc
     3961 gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac
     4021 tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg
     4081 ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata
     4141 gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat
     4201 atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt
     4261 gaatttcctg ccaacttttg cgaaacagct cccggtgttt ggttatttta cctatcttaa
     4321 tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc
     4381 attttccttg actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt
     4441 gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt
     4501 ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct
     4561 tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca
     4621 tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg
     4681 atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga
     4741 ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt
     4801 tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc
     4861 tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga
     4921 agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt
     4981 ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca
     5041 tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt
     5101 tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt
     5161 gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt
     5221 ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg
     5281 ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg
     5341 cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc
     5401 catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct
     5461 gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa
     5521 atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc
     5581 ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg
     5641 aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc
     5701 tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg
     5761 aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc
     5821 tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc
     5881 aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag
     5941 atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg
     6001 attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt
     6061 cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt
     6121 cattgttctc tccattcctt tcataccgga atattccccg gctttacttg tttttttctt
     6181 tttcaatata aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt
     6241 gaaccagtcc tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt
     6301 ttctatatat gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca
     6361 aaacagaaag gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga
     6421 tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc
     6481 tactgaagaa gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca
     6541 tgatttttat aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg
     6601 tgctttgatt tcgtttagtg ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc
     6661 aaaagaaaat ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt
     6721 ttcattagaa gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta
     6781 agcagataac taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag
     6841 tattctccgc ctctattttt tttattcgta caatctcttt tttacagttt ataaaagcag
     6901 tcttctcacc ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg
     6961 acacattggt gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg
     7021 aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg
     7081 acctctgctc ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct
     7141 tattttcaaa tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg
     7201 atctgggctg gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca
     7261 ggaacgtttc aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta
     7321 tttttatatc ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg
     7381 atcccagtca gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc
     7441 aaagtaattt ttctaggtag attcatcagg aatatgatcc aaatccctat tcaaataatg
     7501 gttgtttatt catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga
     7561 ttttacgtac taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac
     7621 agactgcacg ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga
     7681 caaatcttgc tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt
     7741 ttgaaactct tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc
     7801 ccgttagctc ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca
     7861 ggtttttagt tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt
     7921 ggtagctgac attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg
     7981 ctaattgaaa aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca
     8041 tatcaccttg cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata
     8101 aaaatccccg caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc
     8161 agaagcgatt aatatagcgt tgtttttttg tcaatctcta tcaatccatc cctctttttt
     8221 tcattctttc tttattataa gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa
     8281 aataattctt tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact
     8341 atctcttata tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc
     8401 atggacggca aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag
     8461 ctgtgcttag gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca
     8521 tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt
     8581 tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag
     8641 gatgcttttt ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt
     8701 cgatttttgt aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt
     8761 tttcagcaat gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac
     8821 cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta
     8881 ctaattgttc atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc
     8941 cagtcgtttg gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc
     9001 ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaagc
     9061 agcctcctgc aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat
     9121 gattttttat tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt
     9181 gagttgtttg aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca
     9241 tttactaagt cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc
     9301 aacctaagca tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag
     9361 tttctcttga tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga
     9421 cttctagctt ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag
     9481 aaatcgatag gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa
     9541 taaaatttat attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt
     9601 tgattgactt tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta
     9661 agagctggta tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat
     9721 acggtcgaac caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt
     9781 ttctttgttt tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct
     9841 tctctaacca ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt
     9901 gataacctaa attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc
     9961 catttagatt ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt
    10021 cctgattcaa tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt
    10081 tttttgtcac cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga
    10141 tctcaggaaa gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt
    10201 tattgaggga tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg
    10261 gctttttaaa ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat
    10321 cattaccgcc aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt
    10381 cttttgtttt tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt
    10441 ctgtttcaac cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga
    10501 atcctccttg cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt
    10561 agtgaagttt ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg
    10621 ggccagcttt cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg
    10681 ggaacaaaac gaggatcaaa tgtcgtttcg ttaatttcat tttttcacct cttgtattcg
    10741 attggttcta atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac
    10801 aattattttc ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg
    10861 gcttatcctc ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta
    10921 tgcgtagcga taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct
    10981 tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt
    11041 tgtcgcacat ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac
    11101 gttttctttc ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc
    11161 acgcgtatgt tcaaaacact agacagcaaa ccagttgttc ggctgattct tccacctttt
    11221 accaagttat ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg
    11281 acttcttcaa ggattttctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt
    11341 acctgaaaag ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt
    11401 gttagattac tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg
    11461 atcgaaatca cttcactgcc gtcttctcct agctcatcat acatcgagat gaattctcca
    11521 attggtggtt ggcttgtttt gggcagattt tttgctttcg ccatcatttc cataaatctt
    11581 tcgccgtcta gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc
    11641 atgacacgaa tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt
    11701 acgattttta catttgtcat tattttctct ctctctttct ttaacaagat gccttattca
    11761 tgttaaacta catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc
    11821 attaattaca cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta
    11881 tgaaatatta aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat
    11941 cgcgggacta gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt
    12001 ctcttatgct atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca
    12061 ttcattgatt tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt
    12121 tcttttgatt gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg
    12181 ttggacttta tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt
    12241 gacgttgcat aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg
    12301 gtggctctgt gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat
    12361 tttgatggta gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc
    12421 tatccgctat atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta
    12481 cttctctgtt ttattcttta cttaataaaa aaaataaagc tatggatggg acagctctaa
    12541 ataacagcta gtctatccat agctttttta tatcgtcaat tcgcctgtcc cgctttttac
    12601 aggattctca ttacgctgat acgtttcaaa ttgatgcgga taacgatttt tttcatccat
    12661 ttctccttga ctagtattga tcagtgacca atcagaccag tcaacaggtg gaaaataagc
    12721 atctccatcg aatacatgat ggatgactgt acggtataat atcgtacaaa atggcagaaa
    12781 ttcttgataa acagctgacc ctccagcgat gaaaaagata ccgtctgttt ctttttccag
    12841 agcaagcacg tcttccacac tatgcatgac atgcacgcct tctgctttat aggaagaatc
    12901 tctcgtcaaa acaatcgtct gacgattcgg caacggacgg ctacccatcc cttcgaatgt
    12961 ttttcgtccc atcactagtg tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt
    13021 tggcaaacgc caaggaagac ggtcttcttt tccaatcaat ccgttttcat cctgtgccca
    13081 aattgcagct aacattttct catcctttct gattctaaac agcgatagga gctttgatcg
    13141 ccggatgtgg gtcatatcct tctaattgga tatcttccat ctcaaaatca aatactgact
    13201 gcttttcctg attcaaaact aaagtcggaa atgcgcgtat ttctcggctc aattgttctt
    13261 tcatttgttc gatatgattg atataaagat gagcatctcc taatgtgtga acaaattcgc
    13321 caaccgctaa acctgtttca tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt
    13381 tgaacggaac gcctagaaac acatccccac tgcgttgata caattggcag cttagccgtc
    13441 cctcttgaac ataaaattga aacatcgtat gacaaggagg caaagccatg ctaggaacat
    13501 cttccggatt ccatgcagaa acgatcaatc ttctagaatc aggagtcttt ttgattgctt
    13561 cgatcacttg tttgatttga tcgataaagc caccgtcttt tgtttcccag tgacgccatt
    13621 gatagccgta gatgtgtcca agatcgccgt atgtttcagc aaaggaatca tcggttagga
    13681 tcttctcgca aaaaagcttg ctctgctttt tatattcttc attgaattca ggatctgtca
    13741 aacatctatg gccaaaatca gtcatatctg gtccattata gtctggactt ttgatgtagc
    13801 gttcaaatgc ccattcatcc caaatatggt ttttattttg taatagatag cggatattcg
    13861 tatctccttt taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg
    13921 ttgttaacaa aggaaaccct ttttgcagat caaaccgcat ttggtatccg aataagctta
    13981 atgtgcctgt gcctgttctg tcttctttac gatgcccttc ttccaacaat tttcttccta
    14041 agtcaagata cgcttgttcc atcctttttc ctcctaatct tctacccatt cactcaagta
    14101 ttcccaccgt gccatttttt cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa
    14161 ttcttgtaat cgagtgaaat tatctccttg atgattcatc tcttcttgga gcaatgagat
    14221 tttttcttca agttctgcaa tctcatcttc aatcgtttcc cattcttttt gttccatgta
    14281 agataatttc ttcttttcag caggttcctt gctctgtatt tttggtttcg ctttttcagc
    14341 aggctgctgg gtctcttttt gcttcagtaa ataactggac atgtttccgt aataaagttc
    14401 ttgttttccg tttccttgga atactaataa cttatctgct accttgtcca agaaataacg
    14461 gtcatgggaa acagtgatca cagcacctgg aaaacttcta aaataatctt ccaaaatcgt
    14521 caatgttgct atgtccaaat cattggtcgg ttcatccaat aataaaacat taggttgctg
    14581 aatcagcagt ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt tgcctatcag
    14641 tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt aattctgcta ctccgatttg
    14701 actgccatct gctgtttttg cttcttcagc agcttcttgt aagtaagcaa tcatccgttt
    14761 atctggtgcc atttcttcat tttcttgtgt ataatacgca agatgaaccg tttcaccgat
    14821 agacattgta ccgctatcca ggggaattct gccagctaga atattaagca atgtcgattt
    14881 tcccgcacca tttttaccag taatacctag tctttcccga gattgaatca ataaatctaa
    14941 atgttctagc agtgtttggt catttatacg gtaactgcca tctttgattt ccagtacttt
    15001 tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt ttacttggta
    15061 caagttctcc tttagatctt cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc
    15121 ctgtacgcca gccctcatcc aagctaattc ctgtttataa agccgcttgc gcttttcttc
    15181 ttgttctact tctacacgat cacgttctgc tttttctaag acgtaagctt cataatttcc
    15241 tttatactcg tataattttc caaatgaaag ttcgaagatt cggtttgcta ctctatctag
    15301 aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca aatagttttc
    15361 caaccactcg atcgcttcat aatctaaatg gtttgttggt tcgtctagta atagtaaatc
    15421 gggttcatca atcaacactt gcgccagact aactcgtttt ttctgaccac ctgataaagt
    15481 actgattttt ttatctaatt cactgattcc taatttttgc aaaataatct ttgcgttggt
    15541 atctgtagtc caagcatctt ctttattcat ttgttcttct gctttagcat aacgtttttg
    15601 cacatcaggg tcttctccgt tttcccctag ttcgatcaat gcttcctcat atgctcgaac
    15661 agtttggatc aaggggctgt ttccttgaaa gactgcttgg agaacagtta attcatcgga
    15721 aaattcagct gcttgagaaa gataaccaat acggtaatca gttggataaa acactgtttg
    15781 ccgatcaccg tcgctgctat cgataccggc aataatattt aagagacttg ttttacccgt
    15841 cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag aaatctggtc
    15901 aaacaaatct ttttcaccat aagttttttt gaggtcagtt acttttaatt ctttcaacat
    15961 gttccctcct tatcttggat attgtatcat aaaaatagtg aacttcagga aaattttttt
    16021 ggtatttgtt ctttttgtgt tctcaaaaaa agaacaggac cgattcgatc ctgttctgat
    16081 atgttttgtt ttttataaaa cttgaaaatt tttacctttt tattttattg gactcgtttt
    16141 ttttgacttt gctcgaaacg caaatcggaa agaaaagtta tcagatggtt tgttcatcgt
    16201 ataagattct aacaaattga tattcctgcg atagctgttc gcagaaaacg tcgtgatttt
    16261 ttcagcaact tcgtattcat caatgatttt tctttcaaga taataacctc ttagcatttt
    16321 tttcgtaaac agactatctt gctggatcat ccacgaagaa atcaaattcc ccgagttcat
    16381 ttttattcct tcaagtttgc gttgctggat aaaaaattga accatcgttt catcataaat
    16441 atcagtcaag ttatctaaac tttcaataat caactgagta tttttttgat atgtctctct
    16501 gatttcttgg atatcttcat cactaataaa tgtctgacga tcagcatatc ttctttgcca
    16561 aaacattttc ggatggagaa cgattcgcat taacctacgg acaacaatga accaaaagcc
    16621 gataaaagac aaaatctggc ttgtgataga atgttcgaat cgagaaagaa aacgggagta
    16681 aaaacgataa ccattagaag aaagcttgcc cgaacgataa ctttcatcca atccatcccg
    16741 ttcgatcgac aaaatcagtg cttgtatttc ttgtacttct tgcttttcat catcggttaa
    16801 atcttccaag tacagatcac gtatccgttc ttgataattt tctatcactg cttcagtggc
    16861 taaaaattct tttgtattac gatcaatttc ctttatttct tttcgcaaaa cttcaacgac
    16921 ctcttctaaa atcatcaaag cattcaaatc cgtgcttttc gcttcctctg tttccgtcag
    16981 cataggcaag acaatgatcc ctatgaccaa agtcactaag atgacacccg ccgtcagaaa
    17041 caaaagcaaa gaccgctggt aaaacatcat attattgatt gaaaatggca aaataaaaat
    17101 cgtagccaaa ctgactgttc cctttacacc gccaaatgtt aagatcaaga tctcgttcaa
    17161 ctgttttttg aactttttac tgccatcttt gaatacataa aaaagactga tagaaatgaa
    17221 tctgatcaca aaaagcatga cagagattaa tacgataatc gctaataaaa gaccgtttgg
    17281 atataaaccg tctccccata ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat
    17341 aaatactaat gcattcagtg taaaagcaat agttgtccat gtactgtggg acaagctcga
    17401 caattctgca tcaaacacag agattttcct aaaaccagaa gcttggagaa tacctgctgc
    17461 cactgcagca atgattccag atacatcaaa tgcctctgcc aatacatagg ctagaaaagg
    17521 aagcaaaagt tcgatcagca gataccctgt gacatcttgt gctgatgctt tttcgatcaa
    17581 atgaattatt ttttgtttga accagacaag caaaaagccg atcaaagctc ccccaatact
    17641 agaaactact agactcatcc ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc
    17701 aagagcaaac tggaaggcag taacaccaga tgcatcattc aaaagacctt ctccttccaa
    17761 aatatgcatc gcctttttag ggatattcac tcgtccagaa agagaactga ctgctaccgc
    17821 atctgtcgga cctaacgctg ctccaaaagc aaagcaggca gcaagtggga cacttggcaa
    17881 caggaatgat aacgtcgcac ctaccccgac aagggtcaaa atgactcctc caaatgccaa
    17941 gaacaaaatt gtcccgaaat ttttgagaat cgaagaaata tctgcttttt ctccttctct
    18001 aaataacaat ggtgcaataa tcataactaa gaacaattca ggttcaaaat cgattgattg
    18061 tccccactct gtcagaccta ataccactcc aaggaaaatt tggatcagtg gagctggaat
    18121 cattggcaaa atgcggctag ctatgttcga taaggtgatc gttactgcaa atacaataac
    18181 taactcagca aattccatcg tccatcccct ttatttttta ttttgtgtaa gcatttttaa
    18241 aaattgttct tttttcttta aaattttggt ttcgactact tcaacctgtg tttggttccc
    18301 ctctaattca cactgtacac gttttgactc caaacgaagc agatcatgtt tcagacgttc
    18361 caactgacga tacatcaata tatttctttt acgcaactta caaagtattt gacggcggtc
    18421 atccatcgaa agggcgccat agcgatcatg caacagtttt ttttgttgtt caaaactctc
    18481 tctcattttc cagatctcct atacacaaac ttccatcatt atagcaaagt tctttttgga
    18541 tgaaaaatat ttcattgttt catcgtttat tgctagttag aaatttccat aaatttttca
    18601 acgcctcatt ctattccaaa tagtatcaga tatcaagtaa aacttaagaa agataaaagt
    18661 ttctgacaaa aggatgttta ctttcccatt cctgcctttc ttgttctttt atcacaacaa
    18721 aaaaatgggg ctgcgacaaa aatcttgtca cagtccctta ttctgaataa acggtgtgga
    18781 agagacagcc tatcagtagt aataagacac atctctttca cagcctcatt tttgcgtaaa
    18841 aatcgattta ttcgaaaaat tccccgtata catcttctac acgttgatct ggattgatca
    18901 cgatatacaa gttacctgtt ttttcgctga tttttgttga ttgatattga ttgtctaatc
    18961 cttctgttcc tttatctaga tatgggaagt acacaagatc aggtgtgcca tttcggtaaa
    19021 ggatttccat tcgctcgata tcttcgtatt tcaatgcgcg attaaacatg cccatttcca
    19081 gtccgcctag gttgatatca tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt
    19141 taaatccagc acacggatgg atttcaacaa attcactgcc attgattcgt ccaaaacttg
    19201 ttgtgatttg tttgatccaa agatcgccga tatgacggcg gttgatcgtc caggtttctc
    19261 catttctaaa ataaaatttt agcgctgtca tttctttact cattaaaaag actcctttat
    19321 gatgtaattt attcttgtac tacctcagtt tagcacaaat ttatgtaagt ggtcacatct
    19381 tatgtttttt cttctaatga cgtatctgcc cactttaagt agccaacaaa gctgagcaga
    19441 tacagtctag tgttttttaa atcaattttt ctttcgataa aaaatccaaa acctgttttt
    19501 tatcattttt tattctttgt tctacgacgg ctttttcagc taattgcaaa gcttcactaa
    19561 cccacttacc aggttttttt ccagtttctt ctagcaagat tttaccatct actgctaatt
    19621 cgcttaatga cttgataggt aaacgatcat aggattctcc tacggcatcc atctgaggtg
    19681 tttcgtcaaa atagatcatc aattcttcta cttgcagtgc atgttttttg cctaagaagt
    19741 acaattcgaa tggttccaaa gctccagttt ctcgttgtcg tagtccttgg acaagtgctt
    19801 gaacgtcttt aatcatttgg ttagaacact tccacgattt caaaaaggaa cgaatctcat
    19861 tttcttttag atccagttgg ctgatcaata atgcccatgc ttggctctct tctttcaatg
    19921 ggacggatgg taattcagca aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac
    19981 agcaaatgta gcactctgtt tcaataaatg catggattcc cgcggcccgt tcatttccca
    20041 agagcatctt gatgaattcc acatttatac gttctacaga tattttacct aatagctcgt
    20101 ggttttcttt gatggcgtag aaggtttctt cctctaattt gaaccctagt tgactgacaa
    20161 aacgcaaccc gcgcatcatt cgtaaggcat cttcatggaa ccgctcatga ggattgccta
    20221 cagctcgcaa gatctgttct tctagatctt gcagaccatc gaacaaatcg atgatttctc
    20281 catcttcttt caaagcaaac gcattgatcg taaaatctct tcttttcaaa tcttctgcca
    20341 acgaacgaac gaattcgaca tgatctggtc tacggtagtc ctgataagtt gattccgtcc
    20401 gaaatgtggt gatctcatat tgttcatcat tttctaatac caacacagtt ccatgttcga
    20461 tcccaatatc tatcgttttt ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg
    20521 cgatatccac atcatggatt ggctggttca gcaagatatc acgaacactt ccacctacaa
    20581 aataagcttc atatcctgca ttctctaatt ttttcagaac tggaattgcc ttttggtatt
    20641 ccagaggtaa ttgttttaat ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag
    20701 ataaagactc agttgcatgg tgactgtttg acaagtccgt ttcagagtct tcaaggccag
    20761 gaccacttca tcttgtttac tcgtccttac gctcaaagcg attatggtct ctttgattga
    20821 atttttccat gtttttttca aaaacttcag ttaagtcgat attcaatgaa ttagccatga
    20881 tcatcgtaac gaataaaaca tcaccaagtt cttctgctac cgagttaact ggttcagaag
    20941 cctttttgct tttttctcca taagtgtggt tgacttctct tgccaattca cctacttctt
    21001 cagttaatcg agccatctga gctaatggag agaaataccc caccttgaat tgctggatat
    21061 attcatccac ttcctgctgc atactgtata aagaacgctt tttatccatc tttttccctc
    21121 caaaactatc ttaccaaaac tttaacgcaa atcctagata agagcaattg agaaaatcga
    21181 atttccatgc tacatttagg aaggaatttc taaatggaaa ttctgtgaaa atacataatt
    21241 gaaagctgga attcatatgg tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat
    21301 aggaacaagt atttatgcat ttggtcttgt ttacttgaac atcgctaatc atttagcaga
    21361 agggggagtc tcagggatca ccttgatctt aagagcttta tttggtattg atccagctta
    21421 tacgacttta ttaatcaaca tcccattgat tttactagga ggaaagatcc tcggtaaacg
    21481 ttccttggcc tatactgttc taggtacact ttctttgtca ttctttttat ggatctggca
    21541 aagggttcca ctagaaatca atttacagca tgatctactg atcgtttctc tacttgcggg
    21601 tttagttgca ggtgtaggaa gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag
    21661 tgatattatc gctcggattt tagaacaaaa ttacggcatc actatggggc gttccctatt
    21721 ggcatttgat atcatcgtct tacttgcttc attaacctat atcgacttga aacgtatgat
    21781 gtatacgttg attgtttcgt atgttttcag tcgtgtgatt gattcgatct tagatggcgg
    21841 atattctgca aaaggtctac ttgtcgtctc caacaaaagc gaagagattg caccactact
    21901 gatgaccggt ctgcaaagag gtgtgacttt cctttctgga gaaggcgcat tctctggtaa
    21961 ttcaaaaaaa atcatctata tggtcgttag ctctagtgaa ttaactgaag ctaagaggat
    22021 cgtccacgag attgacgaaa aagctttcct ttcaatcatc aatgtgcatg aagtggaagg
    22081 gcaaggcttt acttatttga agccacagac aagattactg aaaaaactat aagaaaaaag
    22141 acaaagatag gctatgagga aagattccac atccagctga cctttgtctt ttttagtact
    22201 atactatttt taactaattg ttatgttcac ttacccggtt acttttcttt tgtgtctgca
    22261 aaaggatctt tcggttaact tcatcagata atttcaaaaa agcatcccta tctccatctt
    22321 ctagtgcttg atcgatttga gcataaagca tttttagttt tgcctcttct tctttttctt
    22381 gaaaatatcg ttcaattaat tcagtatctt cttcactgac tgaagaatcc caagaagaaa
    22441 atggattgtc ttctaagata gatagatatt ctcttgtctg ccaagcattt tcgaatatac
    22501 attccacata taaaggcttt ttccaattca aacggatttc atgaaaaatc tggtctgtgt
    22561 ccgtaaattc ttttccctgc aaaaacaatt tcatgggttc cccttcaaca gatagatcac
    22621 tgatctgcag tcctcgatcc gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa
    22681 tcgcttcatg attggcaaga taattcaaga tccaaaaaac ttctcttcgg ctaaaagata
    22741 catgattgac catccacgtt aaaaaattct tcttttcccg tacatcaatt agcatttttc
    22801 tcacctctct gttaggtcat ctaataatga cagtgcctcc atatctcctg gttcaagtgt
    22861 taaataataa gtcaataatt cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa
    22921 ggcatactct ttcaagaaat ctggttcatg tgatagttca tgataagcct gttcatagtg
    22981 tacagctgca acggtaaagt cttctaattc attataagca tgggctaggt tccattctgc
    23041 ataaggatta gcagtttctt ccatctgatt gatcacttgt atcacatctt catatctttc
    23101 atcatccaag taaagattac ttaaagtaag taatgtttca tcttgtttgt ctcctacttc
    23161 taaagctttc agtaaaagtt cttctgctcg ctgtctatca tgcagacgga aagcattttc
    23221 cgaggccagg tgataaagtt cgacttggta aggattttct ttgattcctt cttctaacac
    23281 tgtttgtgct tcttcaatca tttcttcttc ttgaagagct tgtcctagat atagatataa
    23341 cgattgataa tgcggattaa cttctcgaag ttcttccaga tagttgattg cctgctgatt
    23401 ttctttcaat tgaagattcg taaaagctag ctggaacagt cgatcatctg tcctttcttc
    23461 tttcagtgct tcttctaaag gtacgatagc tgcttcgaat tctccttgca tacttaagga
    23521 ttgtcctaaa cgttcctgca tcgagattcc tgaaatttca tttatcccgc ttgccaacaa
    23581 tgtttcataa accactgctg cctctttgaa acgatcaact gaaaaataca attcagctag
    23641 cgcaaactgg atcaatggtt catcaggaag caacccagct gcttctttca attttgcttc
    23701 actcacttca gggattccga taacttggta caaatcagct atcgccaata agctttgcgg
    23761 atagtaatca ctttgcttat ccactttttc caggtattca aaagctaagt cgatctcatt
    23821 atcttcgatt gcaatttctg ctaacgggat gttcagtccg tcattttctg ggtattggtt
    23881 gatcagttgt tcaaaaatct gttttgcttc gatcaaaaag ccaatagcca gcaattcttc
    23941 gcctagttca gctaatatat ctgctgggtc ttcttttaat gctttttcta aaaagatttg
    24001 tgcttgtgac aagtcttcat ctgataaagc ttgcaacatt ttttcgctat ttgtattcat
    24061 ttcaattccc tcttatctca ctgacgattt gttgaaatac cgtcattgtt ctcaataatt
    24121 ctttttcgtt tggatgttgg tatccgtcta ttatacctat tttttctaaa acaagtattt
    24181 ttttgctttt ttctgccaaa aaaagaactt tttccaaata ctccatcttt gaaatctgtt
    24241 ccagccagtg gattggatag ccaagcaagt ccagccacac aaaaaagttt tttagttgga
    24301 aatcaaaagg atatgcttgg agattccaca cgaaatgaaa catcattcct aaaaattttt
    24361 tcatactagc agaaagtaga tggccatttt cggtcagata aaaagcttgt tcaaacagtt
    24421 taccataatt tgacaggttt tctgcctcta attgataaaa atagatcatt tcttcgatca
    24481 tcccggcaaa cggccgagtc gttaattgct ttttatttgg atagttttta tacagatttt
    24541 gcaaaaaacg atgatcacaa atcattccgc aacagataaa agtcaaaaag tcaatctgct
    24601 ttccgtccac ctgccgctcg ctgattgttt gatcataaac gatgcgttct ggcagattga
    24661 tggtttgcag cactacacga tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc
    24721 ggcttagaga acggatcgat acagggatca tccatagtga tgaatcaagg gttgtatgtt
    24781 tttgaaaaaa acctgccaga tccatgatcc cttcatttcc aaatccgatg ataaacatgg
    24841 attgattttc gggatagcgt ttgacgaaat caagcaggtt ctttaattct gttaaatgat
    24901 tgcattgagt gttggcacaa atataccagt cattgtttgc ttgatttgca aataacgggt
    24961 tgatcttatc tgcaaacaaa tcataatagc gctgattcgt taaaaataag attggctgat
    25021 caatggaaag ttctcttagc tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg
    25081 catcttctac ttgataatca agttccatac gcatccctcc tcttcttatt tttattttac
    25141 cattagtaag caaattctag aaaagataag agcaaagctc acctcataac ttgcaaataa
    25201 ataaaaatta aataaaatac atgtgttttc tatgtatgac atacggattc ggaatctctg
    25261 tctcttattt attccttttc tgctaggaaa taaaaagaaa agcacttgta gccctagagc
    25321 cacaagcgct ttttcaactg tcttatttaa cagcgtcttt taatgcttta cctggtttga
    25381 atgcaggtac tttgctagct ggaatttcga tttcttcacc agtttgtggg ttacgacctt
    25441 tgcgagctgc gcgttcgcga acttcaaagt taccaaaacc gattaattga actttttcgc
    25501 cgttagctaa agcatcttga atagttgaaa atacagcatc tactgctgca gttgcatctt
    25561 ttttagttaa atcagtagct gaagcaactt tttcgattaa ttctgctttg tttgccatgg
    25621 atagattcac ctcctccaca agagatatga tagaaatatc atttagttaa aactttctga
    25681 gtggtccaga agtttaaaaa gaaatattga ttaacttcaa tattctgtta tcacgatatc
    25741 atatgaaccg cttaatagca aggtttttac caattttcac atgctttttt tagtcataga
    25801 aacgtaacaa acgaaactgt ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc
    25861 ctattttctc ctacgaggga taattcggat ggacgtccct tcaaaagtaa atgcttttct
    25921 gatttgattt tctaagaaac gttcataaga aaaatgcatc aattcctctt cattgacaaa
    25981 aacgacaaat gtaggtggtt tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt
    26041 tcctttatct gttggtgttg gattgattgc tacagcatcc ataatgatat cattcaacac
    26101 agccgaagag atacgaagat tctggttcat acttacttgt tcgatcattt caggcagacg
    26161 ttccaaccgt tgtttagtca ctgcagaaac aaaaacgatt ggagcgtaat caagataccg
    26221 gaattcatca cgaatctctt gttcgaaatc acgcattgta ttggtttctt tctttacaag
    26281 atcccattta ttcacgacga taataattcc acgaccagct tcatgggcgt aacccgctac
    26341 tcgcttgtct tgttcccgaa tcccttcttc agcattcagt accatcaaaa cgatatcaga
    26401 acggtcaatg gcacgcattg cacgcatcac gctgtatttt tcagtatttt cgtatacttt
    26461 tccgcgttta cgcataccag ctgtatctat cattaagaat ttctgaccac tttcagaaac
    26521 gaagtgggtg tcgattgcgt ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc
    26581 ttctcctaaa atcgcattga tcaaagagga tttaccaaca tttgggcgac caatcaaact
    26641 aaacttaatc gtgtcgtcgt cctcttcttc agtctcttca gagaaatact tcactgcttc
    26701 atctaaaata tcaccaatcc ctagtccatg gcttcctgaa acaggataag gatcgcctag
    26761 ccctaaggaa taaaattcat agatatcgtt tctcatttct ggattatcta ctttattgac
    26821 tgctaaaatc actggtttat tacttctgta taaaattttc gcaaccatct catctgcgtc
    26881 tgtcacgcct tcgcgtccgc tgactacaca aatgattaca tctgcttctt caatcgcgat
    26941 ttctgcttga tgcttgatct gatccataaa aggttcatcg ccaagatcaa ttccgcctgt
    27001 gtcgatcacg ctgaattctc gacctaacca ttcacctttt gcataaatac gatcacgagt
    27061 tactccagga gtatcttcga caatcgagat acgctcacca gcgatgcggt taaaaatcgt
    27121 agacttaccg acgttcgggc ggccaacgat tgcaattgtt ggatttgcca tattatttcc
    27181 tcctcacaag tttccaatct cacttagttt aacgaatgca ttgccaactt tcaagcatag
    27241 attgtcaatt aaacgaagta tttttattcc ttattgaaat actaatcgct tttcaaaaga
    27301 aaaactgaga caaaagcagc tgctttcatc tcagtcacag atacaaaatc aattattttt
    27361 cagaatcatc ggttgctttt tcttctactt cttctttcaa cgcatcacct aaaatgtctc
    27421 ccatagtaaa tccagtattt tcttctggta attcataaga ctcaacattt tttggttctt
    27481 cttctgaagc tggtttttct tctaaagctt tgatactcaa tgcaatacga tgttcttctg
    27541 gatggatctc taatactttc actttcactt cgtcaccttc atgaagcact tcatgtggtg
    27601 tcgcaatatg tttatgtgaa atttgagaga tatggactag cccttctact ccagggaaca
    27661 cttcaacaaa ggcaccgaag cttgtcaaac gtttaactgt tccgtctaat actgtgccaa
    27721 ctggagcttt tgtatcgatt tctgaccaag gtccaggctg tgtgtctttg attgacaagg
    27781 aaacacgttc ttgttcagga ttgatagaca acactttgac atttacatca tcattgacag
    27841 ccaatacatc acttggtttt gctacatgac tatgtgaaat ctctgagaca tggactagac
    27901 cgtcaattcc gcctaggtcg acaaatgcgc caaaatcagt aagacgtgca acacgtccac
    27961 tgagaacatc tccctcatgg atagttgaaa gaatttcttt tttcttttct tctctttcgt
    28021 tttccactac cgctttatgc gataagatca aacgattttc tgatggttcg atttcgatga
    28081 ttttaaagct caatgttttg cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca
    28141 ccattgatgc agggacaaaa ccacgtacac cgacgtcaac gactaaaccg cctttgacaa
    28201 catttgtaac aggtgcttcg atgaccttgc cctcttggaa atctctttcg atatcttccc
    28261 aaactttttt agcatctaaa cggcgttttg aaagtagata gctgccgttt tctttatctt
    28321 tgccgatcgt actgataacg actaaatcta atacatcacc gacttttact acttcgctga
    28381 tatcttctac cggcaaagtt gataattctt ttgccggaac gactccttca actccagccc
    28441 cttcgattcc gacaatcact tgtttgtctt caatgactaa aacttcgccc ttaacaacat
    28501 cgccaacttt tacttcttgt acgcttttca ttgcgtcttc cattgactgg ttgttctctt
    28561 gattttgatc aaattctgtc ataacacttg tcctcctaac caacattctg ccgtaagtac
    28621 ggtactgttt ttagtttaat tgattattca aaaaaaataa agcgatttcg ttcgttttcc
    28681 ttgaaaaaac aattgttttt catttttttt cttaatttaa aaaagaagat aacctttttt
    28741 caaaacaaca gcttttattg ctgcaacgac ttctggaatc gatttgccag tcgtatcgat
    28801 acgaacagca tcctctgctt gcttcagcgg agagacgtcc cttgttgaat cataatggtc
    28861 tctttcctgt atctctttcg tcagtgtttc aagatcagtc ggaattcctt tttcttggtt
    28921 ctctttgaaa cgtctttctg cacgctcact tacgcttgct actaaaaaga ttttcacttc
    28981 tgcattcggt aatacagctg tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc
    29041 ccctattttt cgttggacag caactaattc ttctctgact tttccatgtg cagagacttc
    29101 agaaacagct tttgtgacat ctggttgacg gatttctaaa gtcacatctt ttgatgctat
    29161 gaatacatgc tgtccatctt cttgttgcga aaaagagata ggatactttt tgatcagcga
    29221 aactagccct tcttcatctg aaaaagatac attgttcgta atagctaaat aagttactgc
    29281 gcgatacatg gcaccggtat ctgtataaat ataattcaat tcttttgcta atatcttggc
    29341 aacggtactt ttcccagatg aagcaggccc gtcgattgca atactgattt gtgtcatgat
    29401 ataactcctt tttctcagaa aaaaagccgc aaagcgcggc tattattatt taattcttaa
    29461 ttcttgacca gggtaaagca taaagttgtt tggatctaag ccattcaatt caaacaattg
    29521 atcagtggtg atccctgcac gctcagccac ttgttttgga ccttcgcctg cttgaacagt
    29581 cgttgttgaa ccggctgcag ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga
    29641 atcccctgct tgataagttg cctcacttga ggaagtcggt gttgtctctg ctgcaatact
    29701 gctttctgac gttacacctg tatcttgaga cacagtagat tcttccgcga ttgtactaga
    29761 ttctactgtt gaagtctcag ttgactcctc ttttgttgta cttgtagaac tttctactac
    29821 tgatgtcgaa gtttcagaag aagttgctgc tgttgcattg ttgcttgatc catttctgat
    29881 ccagaaataa gcacctgctg gaatagcgat acataagacc agtaaaatca aaaatagcga
    29941 aagaaataat gtatttcctt tcttttgttg tctctgagaa cttcttgatg aaacttcatc
    30001 atctgtatcg tagattggtt gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg
    30061 atgtctatct tttctgctca ctgttttacc tcctaaactc tatcgtgtgt attataccat
    30121 aggaaaaagt gtcttgtcct aaaaaatcaa tgttccatgt tttttttaaa taaatttagt
    30181 aaaatcgttc cccaatgaag ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt
    30241 tctgcttttg ctccatcaat atcacaacaa ttctgcggct tttcggagag tttttcgcca
    30301 aaataagcta agatgaattt tctgcggcag ttttcttcat taatatagcg aagcatctgc
    30361 tgcaggcgga attctttttc tttttcctga cgtttgagcc gttccaccca attttctggt
    30421 ttttcagact gctggatcag ctccatccat tttttttgca gctcatcaaa gggagcttgc
    30481 tctgtggcat attccaaata aagctcaaaa ctttgtcgct gttctctaga aagttgatta
    30541 aagaaataat ggatacgttc atccccactt tgataaaaaa gaattgctgc actttcttcc
    30601 tgatctctgc ctgctcgacc gatttcttgt acatagtttt ctagactgtc tggcaaatca
    30661 taatggacca cataacggat atcagattta tcgatcccca taccaaatgc attcgtagca
    30721 atgagaaact gcagctgatt ttttacaaac tgctgttgaa gttgtcttct ttgagctgca
    30781 tcaagaccac cgtgatagta tccaactgta aaacgttccc tgaataaatg atacaatcgt
    30841 tcgacttctt tttttgttgc acaataaatg attgccgctc cttgtgctct ttccatgaag
    30901 tgttcaagtt cttgttcttt ttgctgcgtt ttccgaacaa aaagcgagat attttgccga
    30961 ttgactgatt gtctgatctc ttttggtggt cgatcaaata gtacatgacg tatatctctt
    31021 gctactaaat ctgttgctgt cgccgttagt gccagtgtga ccgggtttcc taaaagcttt
    31081 cggatttttc ctaactgttg atattctgga cggaaatcaa ctccccattg agacacgcaa
    31141 tgagcctcat ctactacata taacgcaata ttttgttttt tcagatgctc taaaagagaa
    31201 ggattagtca acatttcggg actcaaaaac aaaaatttat attgcgatag atgacggagg
    31261 acgtatcttc tttcagattg cgaaagtaaa ctattgaaag cagctactcg tttctcccct
    31321 cgtttttgta attgcgtcac ttgatcttcc attagagaaa gcagcggtga cacgatgatg
    31381 accagacctt ccattaaata ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta
    31441 ggcaaaatag cgagagtatc ttctccatca agcaacgcct gaataatttc ttcttggccg
    31501 ggacgaaatg aatcaaatcc aaaatatttt tttaattcat ttattaacat tctgtatcgc
    31561 ttcctttctc aatagataga tttgatacaa acgaaattct ccataatcta attgtccatc
    31621 tacattccat tcgctaaagc gccactctcg cacggaatcc ttatttggtt ccaaacgttc
    31681 gattgtttct gcagagagaa tccactcgta tggaaaatca tcaaaaaaca aggcccactc
    31741 aataagatga tctgttactg ttcctctttt taaatgacgg attgccatga tctggtcaat
    31801 tgtctgacca ttcataaaca tctgcttggt gatcatcatg ctttgattga aattttgctg
    31861 taataatgaa tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa
    31921 caaaaggtgg atacatctgc tttgttgcag ataaaccgct gactcatcat tgttttttgc
    31981 cagctgataa ggaagcagcc ctgttcgctc atttccagat aattggttag ctaaaaaatc
    32041 tgctgcttct tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat
    32101 caaaatttca cgtggcaatt ggctttcact aagccatttt tttagttgga aagtataaaa
    32161 aggacttgtc tctgctggaa gatagtcttg aatacccgtt gccagattag aaattacttg
    32221 aacagcaaat ttaatgagcc gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg
    32281 caaaccagta tgttctattt gatgttcgga caacatatca gcccctgcag gcgttagctt
    32341 tgcttcattt tcatttattt cgatccaacc ttgctgcgca attttttgca aaatttgata
    32401 aaacttatcc tgcttcaatt ctggaaaact gttatggata aacaaaagtt cgtgggtaaa
    32461 tccataaata agtactgaag tcgttcgttt tcccacaagg agttggtaaa gagaactaat
    32521 ccgcagcttg tgtcctgatg aaaatagcga taaaataaaa acaatcatat tattctcctt
    32581 aagttaggat ggttagaaat gtcactacta tgtaaacttg tacctgaacg ctgtattgca
    32641 tgcggtcttt gtcaaatcga agcgccagat attttcgatt atgatgaaga aggaatcgtt
    32701 ttatttgccc aagaaccaga ggctcatcaa cagttcgtga tgccggaaga agaaacatat
    32761 gtaaaaaaag cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa taaaatataa
    32821 atataaacga aactttaaaa acgtacacta agttctctaa acttgaaaat aagccgggat
    32881 ctgtttctct tgttccgtga aatcccgact tattttgaaa gattcatttg ctatctatag
    32941 ttaattgact agttttttca cggaccctat aggacccatt tatattattt caactttttc
    33001 agccagtttt ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga aatttctgcc
    33061 tctccatctc ctttttgctc accatagctg ccaaaacctc catgatttcc gccattgatt
    33121 gatacatact ctgttctttc cggtaaatac tgcttagctt cttgataagc ttcctgattc
    33181 aatactttat cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt
    33241 ccttttttgt cgggataact tgccatgaag aatacccctt ttaaatgttt gtctgaatga
    33301 ttcttattga cataacgact tgccattacg ccacctaatg aatggccacc gatcacataa
    33361 tcggaatttg gaaaatcttc tttgacctta tccgctttgt ttccgcccat cactgctaga
    33421 tcaagtggca ttttgacaat agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa
    33481 atactataac tttctggctc tacgagagca ccaggataaa aaataagtaa tggagacttt
    33541 ccatgattct ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt tccttctttt
    33601 accaacactt gcgcttctga cgatggttcg tacgtatttt gctgtatata gtaataaccg
    33661 gctccaccga gtaatcctaa aatgatgact aagccaatta gtattcgtct aaatattttc
    33721 actttctccc accttttcta tctgagtgac ccctttttct taatcaagta tgattatacc
    33781 ttaaaaaaat aagagagaaa tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc
    33841 catttgaata tccgcccttt ttgtatgttt atttttattt agcacataaa cgatacacag
    33901 cttctgcata aatttccatt gctttataca tactacttaa ttcccatcgt tcattcgcct
    33961 ggtgcataaa gtcaggagta tcagggaaca tcgcaccgaa tgccacacac tggttcatcg
    34021 tacgagcaaa agtagcgccg cctgatacaa acggttcagt catgtctcca gtaatatcgc
    34081 gatatgtccc caatagattt ttcactaact cactgtctag tggcacgtat aatgaatcaa
    34141 ggaagtcaaa ttcttcataa gtcaacccat atttttctgc tgttttagtt aatttagcta
    34201 ctaaatcatc tttcttgaat gtaacaggga ttctcatatc tacaccgatt tttgtttgtt
    34261 ctggtgtgat ttccaaactt gcaaagttca tcgttagctc gccggattgt tcatcttctg
    34321 ttttgcccac gacagcttca ccagttgcat tttcttgaac taattttcct aagaaattga
    34381 ttgggccaaa atcaaatact tctgaaagag caattgccag acgagaaata gcattcactc
    34441 cttgtgctgc atctttcgcg tggatacttt taccaagaac gacgatgcct tcaccttgtt
    34501 cttcgaaatc gaattcgtgt tttttcaatg cttcttttac ttcagaaagc ttttctccag
    34561 agtaaggagc tgcatcagga acaacattca acgctcctcc agcttttaca gaaaactcgt
    34621 tcgttcctgg tcctgtcaag taagcttgca ataagccttt ttctgcataa attaaaggaa
    34681 attcagcatc tggcgcaaat ccttgcgtaa ttccttcttc tttttcgtta tatttttcta
    34741 aacaacgcca taaattttct tcatctgtac caaagataaa acgaatacgt gtcttgaatt
    34801 ccacgccagc atcaatcaaa gctttgacgg catacattgc cgcaatagaa ggacctttgt
    34861 catcttggga tccgcgacca actaaccagc cgtcttttat agttggatcg aatggttttg
    34921 tttcccaatt attttcgtcc cctgctggaa caacgtccat atgacacaag ataccgaata
    34981 attcatctcc agagccaatc tctgaataac cgtaatagcc ttctggatct ttaaatgttc
    35041 taaatcctag cttttcactg atttccaata ctttatccaa agcttcaatc acttttttac
    35101 cgaagggatg tccttgtcct gaatctgctt catctagtac ggaaggaatt cggatcaatt
    35161 cgctcaatga ttctaatgct tgttcatgat gttgttctgt cacaaatttt ttcatgatat
    35221 tatcctccta atatgttaaa aagggtcatg tcaaattcct ccgacacaac ctcttcttct
    35281 caaataatga cgttttaagc ctgctccatc agcaagcaga atgttcttaa caaatgatga
    35341 aagaaaaaac actcactgaa tggagctatt gacttatcca cgtgcttatc gatagccgtt
    35401 tatcggtttc ttcattctta tgatagacct tgtacctgta tttgtctagg tttagaacat
    35461 tgctgctaca cctaggatca cacagatggc tacaaataat atcgcaatta atttaccggt
    35521 aaatttccac cacactgaaa catcgaaacg agcaattgct acagcaccca ttacgatagc
    35581 agatgttggt gtaattaagt ttactaaacc agatgcagat tgataagcag tgattactaa
    35641 atccttaccg acgccggcaa attcacccat aggtcccatg attcccattg ttgcagcagc
    35701 taagccggaa gttgaaggaa tcaagaatga catagggata tagaaaataa atgtaataat
    35761 gatgaataca cttgaagata atgaacttaa tccttgttcg ccccaatgta agatggtgtc
    35821 tgtgattaat ccattgttca taaccacttg gataccacga gcaacagcta ctacgattgc
    35881 tacaccgatc aaatcagaag caccagctaa aaattcagag acaaattcag actctttcat
    35941 gccataagtg attgctacta cgatggacat tactaagaat aacatagtaa tttctgggaa
    36001 gtaccagtca cctaatggaa gcatgctttt acctagaacc gtacctagta ccggaaggtt
    36061 tgttaaccag tttgtcaatg attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg
    36121 aatcaaactg atgatcatga taccaaaagt aataaagaac aatacattaa catgtttttg
    36181 ttttttcgtg attacttctt gacgaccgat tgcttcaata tcgaaatgct tcatatcttc
    36241 tttacgttgg ttgtatacta atgattttgt tggatctttt tcgatctttg atgcataacg
    36301 atacacatat acaatagaaa tagctaacaa aataacgaac atcaagaatc gaagaccaat
    36361 accttcaccc atcgaaatcc caacagcttg tgaagctacg cctgttgcaa aaggatttac
    36421 cgttgaagct agacagccta cttgagaccc tactaatacg attgctacag ctactactgt
    36481 atcaaagcct acagccatca tgactgggat cagtaaagca tagaatggaa tcgtttcttc
    36541 tgccattccg taagtagagc cgcctaatgc aaataacagc atcaatactg gaataagcat
    36601 tttttctttt cctttgaatt tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt
    36661 tttgttgata atacctaaaa atccaccaac aaccaaaatg aagaaagaga ttgggattgc
    36721 agcaggtgtt tctttagtta gctcccccgc tggtacacct agcatcccat tgattggcgc
    36781 catgaaaata tcccacagcc cttgaggatt ttgttgtact cgttcatatg atccagcgac
    36841 gatgttgcct gcatcgtcta cactatagtg cccagctggt ataaaccaag taagtacagc
    36901 gattactgca ataataataa ataagaccgt atacgctgaa ggcatttgaa attttttctt
    36961 ttttgtttgt tccatcttct tcttcctttc ttttccgtta gagacaacaa ttttggctct
    37021 aatgttattt tatcaaaaag agtatcagaa caaaaataat aacacgcatt ttgtaaaact
    37081 tttgttcaaa aagttcgttt ttccctattc cctcctcttt tttcaggttt ttaaagcttt
    37141 cttctttttc attgtagcgt ttcctttttt ttataaacaa acattttcgt cttttaaaag
    37201 gagaagaatt tcacatttgc aaagaattga ttgattttta tgcataaatc aaaaaaatac
    37261 tcaaaacaaa caaataaaac gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa
    37321 aagaccaaga aatttcttga tctttttgat atttatttga ctgctgtatg acgttgttcg
    37381 aactgatgtt gttttcgact taaccatgga agaagttgcg catgcagcac taaaaagaca
    37441 gcactcacga tgaatccttt gatcaagttg aaaggaacga tcccaatcag tacatagttt
    37501 gctaaagaca tgcccagcat ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc
    37561 gtaataacga aataatttgc gatactcata aaaatagtta gagcgatggt tcctgtaatg
    37621 acacctaaaa atttgtttct tcctttatgc tgtttcttta cactaaagaa ataataaatc
    37681 gggaatgtaa agatcgttgt tgataagaaa ctagcagtgt ctcctaccaa attatccgga
    37741 gaaaaacctg tagtcagcag atgtaatact gaacgcaaga acgctgttgc aactccggcc
    37801 gctggaccga ataagaacat gctgatcaat accgagatat cactaaaatc gatcttcaaa
    37861 aaactaaaag ccggcatgat cggaaaagca aaatactgta aaactaatgc gatcgcagca
    37921 aacatcgcta ctgcaaccat tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt
    37981 aggactcctc ttcagcgaac tttttgacgt ttctctccaa atgaaaaaac tctattttcc
    38041 agggaaaata gagttagagt tatttgatca agcgtcgtca aataagctct atcttcttta
    38101 tccagactat actgtcggta tcggaatttc accgattcag ctattttttt gcaaaatagg
    38161 tcgtggacta taaccaccgg tcgggaattt caccctgccc ctgaagacga accttatata
    38221 atttttgttt acatattcat tatacatatc tattcaaaaa acgcaacatc taacacttat
    38281 aaaaagtaag tgttcatttt gagtggttca gcaaactgct gacttctttt ttgttcagtt
    38341 cacggtaatc cccaggacgt aggccttgta aagtcagatc tccgtatttt tcacgtttta
    38401 gtttttgcac aggaagacca actgcttgaa gcatattttt cacttgatga ttacgtcctt
    38461 cgtggatcgt caattccacg atactgtggt ttgtcttagg atccactgat aaaatttgga
    38521 atttagctgg agatgttttt cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca
    38581 tcgattttgt agcgatccct tttacctttg caacgtaaac tttatccact tcatgttttg
    38641 gatgtgtcag gcgctgagca aaatctccat catttgtcag tagcaagatc cccgaagtat
    38701 cgtaatccag tcgacctaca ggatagatac gttcttttac catcggcaaa agatccgtaa
    38761 caacttttct tcctttgtca tctgcaacag cagaaataac tcctttaggt ttgtacaata
    38821 aatagtaacc gtattcttct tgataaattg ggacttcatc cacttccaca gtgtcatgtt
    38881 tcccaacttt tatccctaat tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa
    38941 ggatgaattc ttctgctttt cgacgagaag tgatgccagc gtgggcaatt actttctgca
    39001 atctctccat tacaattatt ctcctttatt cattccatca aagaatagat ctggtgacac
    39061 gtcttcttca agtgaaattt ccatttcttg tgtatcaggc agttcttcta atgatttcaa
    39121 accaaaataa tctaagaagt agccagttgt tccatataat atcggccgac ctggtccttc
    39181 tactctacct ttttcttcaa tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac
    39241 ctgaactcct cgaatctgct cgatttcagc tcttgtgatt ggctgtttat aagcgatgat
    39301 tgctaaggtt tcgacggcgg cttgagataa tgtgtttgat gtcccttgcg catatcgctt
    39361 aagtaaggga gccagctctt ttttagtcgt taatacataa tggctttccg tttcaaaaat
    39421 atgcaaagct gagtgcgagt tttgagtgta agattctttt aattctttca ctaaacttgt
    39481 gacttctgct tcgctgagat ttaacagtcc gcttaattct tcattactga tcccttcctc
    39541 gccaacaata aaaagaaggg cttcaagttc acttaatttc gtcatgttcg gtatctccta
    39601 ctgctggata aagcataatt gattcatagt tttctttttg ttgcgcaatg atctgccctt
    39661 ttttcatcaa ttctagcaaa gccataaaag tagtgacaac ttcactttta gaaggtgact
    39721 caaaaaaaga atcgaaagta cagccatgcg catttctttt tatacgctct tcgataaata
    39781 ccattcgttc ttcgatcgtt gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa
    39841 atttcttacg ttctaagatt ttctgcatgg cacggatcaa atcgattttt ttgacttgtc
    39901 ctcgcttcaa cggcttttca gcatccgcta acccatcgat gttcataggt tcttttgtgt
    39961 aataaagact tcgttcctct gctttttctg tcagtctttc agctgcatat ttatatttac
    40021 ggtattctaa aagctgagtg accaattctt ctcgaggatc ttcttcccat aattcttcat
    40081 cgtctgtgac gaattcttgg acaggaagca atgtcttgct tttgattgcc atcaatgtag
    40141 cagccatcac taagtattct cctgcaacag ctagctcgag tgttttcatt gtatggatat
    40201 aattcatata ttgctccgtg atcgtagcga tcggtatatc atatatatcg atttccattt
    40261 gctgaatcag atgaagcagc agatcgagag gaccctcgaa tacatctaat ttaatattga
    40321 tctccgtcaa ctcattcact ctttcttctt tttctttgct agccattttc caatgtaagg
    40381 ctctgtgatt tcagttgcac ggatggagac atccgggtac ttttcctgta attcatctaa
    40441 catttgaaag cccatattct caccgcgata agacggattc aaagaaatgt catggatgat
    40501 cagctgatcg ttcgttgccc agtaaatacc aataatccct tgaatgttgg ttgaaccttc
    40561 tggcgtaaag aaaaaaagtt cataatcgtc ttcagattcg tacgtatcaa tctccttcaa
    40621 caaagaagtt ttacctgtca gtttcttatg gaaactcaat agtcccatag caattttacg
    40681 gttttctttc cggtaatgtg ttaacatctt ctcaccttcc tatgactaag cccttggaaa
    40741 atgctgctta taaacatccg tcattctttt cttcgtgata tgtgtataaa tctgagtcgt
    40801 agagatatcc gcgtgaccta acaattcctg tactgtccgc aaatcagctc cattttctag
    40861 cagatgtgtg gcaaagctat gccgcagcgt atgtggtgtt acttctttgt aaatcccagc
    40921 ctctcgtact aactgcttca gatttttcca gattccttgc cgagaaagac ctttcccatg
    40981 attattgaca aatacatgca tttcagatgc gtcctttaca agtaatggac gtgcttcgtc
    41041 taagtagcgt tccagccatt ggatcgcata atcacctaaa ggaatgatcc gctctttatc
    41101 tccttttcca actgtctgta ccagtccaag agaaagatga agatctccca atttcaatcc
    41161 aatcaattca ctgacgcgca tcccagtagc atacatgact tccaagattg cacgatctct
    41221 tatccctaac gtttttgtcg tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt
    41281 caatgtactt ggtaatttct gtgctttctt cggcgtatcg atatgttgca tcggatcatg
    41341 atcagtgata cgctcttgcc gtaaaaactg atggaatctt ctgagacttg agatcatccg
    41401 agtaacagtt gctgtcgctt ttttttcttc atgcaacttc tccagaaaac ggatgactaa
    41461 aaagcgatcg acatcctgcc atgaagcaat tttctgctcc tgtagaaaat gcaagtactg
    41521 ttccaaatca cgttcatagc tttgtcgtgt attggcggac aatccccgtt caatattcaa
    41581 gtagtggaga taatcgttga tttgctcttt cataataatc cccctcatgc ctctctatca
    41641 tatcagatac cgcttcattc tgatagtctt tttccataat ctgagcaaag aaaaagccgt
    41701 ctagacactc agacggactg gtttcttttt tctttttcat agcattcctg acagattcca
    41761 tggaacgtta gccgatgatc ttttactaaa aaatgataac gttcttctac tacttgctcc
    41821 acttcaagta aaagatcctc ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa
    41881 tgatgatgga aatgtttggc gccttctttt ctcaaatcat aacgggctaa tccatcatta
    41941 aaacttactt tatcgacaat tttcaattct gtcaagattt caagggttcg ataaacagta
    42001 gcaagtccaa tttccggact cttttttttc actaaaaaga agatttcttc tgcagataaa
    42061 tgctctttct cattttccaa aagcacaagc agagtagctt ctctttgcgg tgtcagctta
    42121 aaccccgctt catgtagctg ctgttttgtt tttttcatcg ccgaaatagc attcatgacc
    42181 atttactcaa ccccaacaat ttataataat tacaaataaa aaaagatata acactatgtt
    42241 aattaaaatt attataagct agttaagaaa aacatgcaag ggattctcaa ttagattttt
    42301 tgactagttt tgtcacaccg ttttcttgtg taatcaatcc ttgtttcaaa agattaccta
    42361 atgcgcgctt aaatgctcct ttgctgatac caaaagcttt catgatttct tctggatccg
    42421 atttatctgt aaagttgatt tgctgatctg cagaacgttc taaaatggtt aaaatcattt
    42481 gtgcatcatc tgatatagct tcatatgctc ttggcttcaa tgaaagattc agtacaccgt
    42541 ctggtcggac accaatcact cgacctttta tctgctcgcc tagacgaggt tcattatatc
    42601 gttcagaagg atggataaac ccaagataga aatcttccgt taaaacgtac gtacctgtta
    42661 atttcaagcg gtatgctgtt cctatcacat ctttgttttt catttcttct gttccatatt
    42721 tgcttaaaga tttgaatatt ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat
    42781 caactctaag ggaaatcatc aagcgatccc cttttttagg ccatagctct gtcattgtcg
    42841 gcaattcatc aagagatacg acaaagtctt tatctggaag accgatatcg acaaacgcac
    42901 ctaaatcttt tcgtgtagca gtaacgattc caaaagcgta atgaccgata cggcttttag
    42961 gaatttgtgt agtgatccga ttttcatgtt tttgattttg gtaaccgaat ccttcaactg
    43021 cttcaccgat tttgtgttca ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt
    43081 ggacaaagta gtttttttca ttttcatcaa taataatacc tgtaaatata gttgctaata
    43141 aatcgttcat ttttttctcc ctctatagtg aaaggttttc ttaatacatt tctttgctat
    43201 cataacataa tgttatctac ttgaatagct gagccttggt ttggaaaggt gaaaaaagga
    43261 gtatgacaac agttgtgtca tactcccctt tttcgaataa acggtgttaa acggcaggct
    43321 cacagcctct ttcatttgcc taaaattaat tgctcccatt tcctccgaac catccagttt
    43381 tacagttgat ggttacgatg taaaatggca aagcactttc tccaatgcct ttcatcaatg
    43441 cagattttgt tgaagtggca tatagattat aatagccttc cccattttcg attctggctt
    43501 tttctaatct gtaacctcct tcaacgatat atccacgttg gatacatgta gcaatcactt
    43561 tgctttggac acctggtgcc cacgtccatg ctggattaga tgcatcggca atatcacttg
    43621 ctctggcaga tttgattggc tgattcgctg agtttgaaga ggaaggggtc gaggatgccg
    43681 cttcttgctg tgctgcttgt gcgaccgcag cttgttgagc ggcttgagct gcagctgctg
    43741 cttgagcggc ttgttttgct tgttcttcag ctgctttttt cgcttcttct gcttttttct
    43801 tttcctcttc ttcttttttc tctttatcag tgagcatctt atctacttgt ttcaattgtt
    43861 cattcaactg agcagataga tctgtatttt tcactttttc tactgcttct tttgccgtct
    43921 tgtattggtc gcgtgttgct gaatccacta cttttccgtc tttaaagact atagctgttt
    43981 tttctttagc tgtttgcaat tcagcatatt gatttttagc ttctgtttgt gcttgttgaa
    44041 tcaattttcc aaaatctgtg ttatccgttt cgattgcttg gatctcttta tctgctttca
    44101 acttgatgtc atctgctaga tgatcatcga cgatggcagg tgctatgaac cactcattca
    44161 ctttttggat atctcggatc ttagattgga tatcttggta agttgtttct aactctgaat
    44221 atcctttttc attcttaaga ctggataaat cattttttag ttttgataaa tcttgatgaa
    44281 tcatatctgc acgaataaat tgatggtgat catctgtata aaaggcagca agtttctcat
    44341 gaagttcttc catttgttgc tgtctttttg cttctgctgc tacttcggct tctacttttt
    44401 gatgatggtc ataataagcc cagcttcctc caccgagtag aagtactgct gctaaaacga
    44461 gataaggtac tttttttgat tttttctttt gcgctttatc tggatctgtt ttttctaagt
    44521 ttgattcttg atgtgttttt tcagtatctg gcacgattgt tattgatttc gcttctgctt
    44581 caaacagctt aggctggttc tcttcttttt ctatttttgc ttcaagatca gtaccctctt
    44641 tgcttacaga ttgattttca gcatcggatt cactatttat ttcatcgggc tctgttgact
    44701 ttttgtctgt gttttcgagt atttcttttt tctctgcttc cggcagttta ttcgtttttt
    44761 ccttttctaa ttgtttttca gtaaatactt cagttttttc tgtgatatcc gtttttacgt
    44821 cttttatttt tactgtttct ttacctgtgg cgttttctct atcagagtct ttttcagttt
    44881 cttgttcctt ttctggctct actttgtttt cctgtgcaat tttttcactg atcattttga
    44941 gcagctcttt cgcgtgctct tcttttgtat cttcacgatg tgctttgata taagcggata
    45001 gtatcgaatt ttcttcctca ttatcgacga tttctttcag ttcagctact cttctttttt
    45061 gttttgattc aagcgtttct tcttgattct ttcctgattc ttccgaagtc tcatgaacag
    45121 ttgctttctt ctgttcattt ttctctatat tagttacttg atcgtgagca ggtggttctt
    45181 cgtcttctgg ttctgctttt tcgaataatt ccattacaga ttctagaggt aaatctttca
    45241 attccgacca ttcgattgga tcgtttaatt ctgtatcagg aaagactgca tcttcagttg
    45301 atttatcctg catagctgca tctgtatttg tttcttcaat tgattctttt atttgctgat
    45361 cttctgcagt tatttgatga ccgcagtctg gacagatagt attctgctcc atttttttcg
    45421 atccgcaatt tggacatttt ttcatcactt agcgatcggc tcctttacta ggtttacgct
    45481 accatttaat ctatcatatt ctatacgtca cgccaatagc ttatttttag gatttactta
    45541 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
    45601 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
    45661 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
    45721 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
    45781 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
    45841 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
    45901 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
    45961 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
    46021 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
    46081 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
    46141 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
    46201 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
    46261 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
    46321 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
    46381 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
    46441 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
    46501 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
    46561 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
    46621 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
    46681 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
    46741 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
    46801 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
    46861 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
    46921 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
    46981 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
    47041 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
    47101 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
    47161 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
    47221 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
    47281 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
    47341 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
    47401 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
    47461 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
    47521 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
    47581 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
    47641 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
    47701 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
    47761 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
    47821 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
    47881 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
    47941 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
    48001 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
    48061 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
    48121 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
    48181 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
    48241 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
    48301 agattcagga tttcggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
    48361 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
    48421 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
    48481 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
    48541 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
    48601 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
    48661 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
    48721 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
    48781 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
    48841 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
    48901 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
    48961 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
    49021 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
    49081 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
    49141 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
    49201 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
    49261 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
    49321 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
    49381 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
    49441 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
    49501 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
    49561 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
    49621 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
    49681 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
    49741 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
    49801 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
    49861 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
    49921 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
    49981 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
    50041 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
    50101 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
    50161 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
    50221 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
    50281 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
    50341 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
    50401 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
    50461 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
    50521 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
    50581 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
    50641 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
    50701 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
    50761 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
    50821 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
    50881 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
    50941 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
    51001 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
    51061 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
    51121 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
    51181 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
    51241 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
    51301 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
    51361 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
    51421 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
    51481 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
    51541 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
    51601 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
    51661 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
    51721 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
    51781 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
    51841 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
    51901 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
    51961 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
    52021 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
    52081 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
    52141 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgt actccttctt
    52201 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
    52261 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
    52321 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
    52381 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
    52441 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
    52501 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
    52561 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
    52621 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
    52681 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
    52741 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
    52801 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
    52861 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
    52921 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
    52981 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
    53041 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
    53101 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
    53161 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
    53221 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
    53281 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
    53341 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
    53401 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
    53461 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
    53521 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
    53581 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
    53641 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
    53701 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
    53761 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
    53821 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
    53881 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
    53941 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
    54001 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
    54061 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
    54121 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
    54181 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
    54241 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
    54301 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
    54361 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
    54421 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
    54481 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
    54541 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
    54601 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
    54661 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
    54721 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
    54781 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
    54841 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
    54901 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
    54961 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
    55021 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
    55081 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
    55141 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
    55201 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
    55261 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
    55321 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
    55381 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
    55441 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
    55501 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    55561 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    55621 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    55681 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    55741 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    55801 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    55861 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    55921 atctacaaag agcgcctatt gcagttgtca aaaatgccca agaagaaatt gtcgcttttg
    55981 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    56041 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    56101 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    56161 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    56221 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    56281 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    56341 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    56401 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    56461 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    56521 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    56581 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    56641 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    56701 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    56761 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    56821 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    56881 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    56941 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    57001 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    57061 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    57121 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    57181 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    57241 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    57301 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    57361 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    57421 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    57481 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    57541 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    57601 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    57661 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    57721 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    57781 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    57841 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    57901 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    57961 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    58021 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    58081 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    58141 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    58201 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    58261 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    58321 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    58381 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    58441 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    58501 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaaagag gctgggacag
    58561 aagcgtttaa ctccaagaaa taagaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    58621 tagagcgtga gacaaaagtg ttctttactt ttgtctcacg ctcttttttt gttatattcg
    58681 tcttttccga tccttctctt gacacaattc atttaactga tcactggtac ttgaacacct
    58741 aatattctag tcatttttat aaacgattca atacttcatc cacatttttg atcatcacgg
    58801 atatcgtacc atccggatta ttcacaaatt caatcaaatc tgggttgcga tatacatcta
    58861 ctggtacgat cagttcgatc ccgtttgata gcttgaattt ttgttttcca aattttttct
    58921 cagaaatttc ttgtacttct tttacaggag gtgcttctgg gacaaatcct tgctcttgta
    58981 cttcttcttt aaaagccatt tttgccgtga tgttttcctt gaagactttt tccgcaatca
    59041 tctcatgatc caaccgacct ttttcttcta tcgtatcata gacagcttct ttcaaatcag
    59101 ccatgatatc aaattcttct gtctcaaatt tttttccaag atgcttggct actttttgga
    59161 tcacttttac attttcttct aaagaaggtg caggaacact ttcgatcact cttccagaaa
    59221 aataaagctc ttttttgcca gaaaactcat attttttttc aattaattcg taggtccaat
    59281 cagtcaaatt cacagcgaat gcttcatctg ctttttgtgt tttcgcccct agaattgctc
    59341 gattcaaaat caagcggttg tcgatccctg cttcgtctgc ttcaacaaag tgagtgtaag
    59401 cctcacgata attgactttc aataaagata agtacatcac tgtatctaat tcatacaaga
    59461 caacaaatac atctgcacta ggtgcttctt cactttcttg gtagatatcg aaccaccgct
    59521 gaacgattgc ttcacttgct tccacaaaat tttcttgagc gattttaagt gattcaacga
    59581 tcatgctttc ttctgctagt tgtccagttt ttgtttgagc agaagagatt ttttggatct
    59641 tcttttgaag aaaatcacga atatattctt ttgtcagatc cagctctttt tgtgaaaaga
    59701 ctggagaacc agtttctctg tcaataatat gcagaattgc tttttttaaa tagatatcca
    59761 ttttctcatt cccttctgtc ttagtttacg gaaaaaaaat caaaaatacc agaatcaaaa
    59821 tcattttctt ttaaacaaaa atttcaagag tagaaacaaa atggattctt tttttcgaaa
    59881 aatgcgctac acttatagtt gaacgaataa aaaaggagct gaaagtatga attcattaac
    59941 aagcacttat cgtctatcaa atggttacga aatcccggta gtaggattcg gcacatggca
    60001 aacacctgat ggggatgtcg ctgtttcctc tgttaaagaa gcgctggctg caggttatcg
    60061 ccatatcgat actgcccaag gatacaagaa tgaagaaagc gtcggccaag cgattaagga
    60121 aagtggaatt cctagagaag agatattctt gacaacaaaa ttatggaatg caaaccacag
    60181 ctatgaactt gtgatgtctt cttttgaaga atcattgaag aagctccaaa cagattattt
    60241 agatctgttt ttgatccact ggccaaaccc tgttgctttt cgtgataatt gggaacaagc
    60301 aaatgcagat acatggcgag catttgaaga actgtatgaa gcaggtaaaa tcaaagcaat
    60361 cggtgtgagc aatttcttac cccaccatct agatacacta gcaaaaacag caaaaattat
    60421 gccgatggtc aatcaagttt tccttgcacc aggcgaatta cagacaactg tagttgagta
    60481 tgcaaaaaaa catgatatga tcctcgaagc atatagtcca ttaggcactg gtaaaatctt
    60541 cgatgtgcct gaaatgaaac aaatcgctga agcacatgac aagacgattg cacaagttgc
    60601 tttgcgttgg tcgctgcaac acgaattcct tcctcttcct aagtcagtga caccaagcag
    60661 aatcaaagaa aatactgaat tatttgattt tgaattaaca gaagaagaaa tgaaacaaat
    60721 cgatcagtta gatggtgtag tcggcaaagc aaaagaccca gatactaccc aattttaagt
    60781 cgaaaggtga ttttatggat ccaatcgaac gcttgaattc tctctcggaa gaagttaccc
    60841 agacttttca ttccgatttt gtctttttga tcgatgcaga gaaaatccaa cattttcctg
    60901 caagaaactg gacacatgat caaatcatcg aagaactgaa aaaacggttc gatcattcgc
    60961 taatggtcaa accctggcac gaacatgaag tcatttattc acctgaacta cctgtttttg
    61021 ccttgatacc gaaaaaatag aaagaatgga acggattatg tatatataca taatccgttc
    61081 cattttttat ttttctttca aaaaacggga aaatacgcaa atttttctgt tgtaatttgc
    61141 gtatacgcaa gtttttttgc attactcctt ttcatttggc tgtttttttc ttataatcga
    61201 aagggtatga gcgaaggaga gaacatatga agaaaaaaca gaaacaaaaa ttattaaatc
    61261 aattccgtcc ttcattggat ggtgtaagag gacaattatt ccgttctcta gaagaagaga
    61321 gcttgcgacg ttatggtttg ccgttacaag tcatgcttga tcccaaaaac cgtcaaatct
    61381 taacgatcgg attagctggg aaaacaaatg aagatacacg gatcaacgtg ccaatcgatg
    61441 acaacttcaa tactgtatta aagagaattc gttcaggaga aaacgggatt ttcgaacgat
    61501 tccgtgacaa tcttcttatt gaaatcgtgt cttactggaa tgatcaacga ttgaaaaacg
    61561 atgaaccaac tgcgcaagct gttgcgacac cagttacaga agaaacagtc agtgtgccag
    61621 aagaaaaaac ggaaaaacca aaagcggaca caactgaaaa tacacgctca actgatacga
    61681 atgccgcttt aagttttgct gatttttcaa aagccgttgc tgagtatcct aaattttaca
    61741 cagaacaaaa tgaacatgaa gtagttatta atgaaaaaaa tgctgatgaa cctcgtaaat
    61801 tagcaactat ttctatgact actgaaaatg agttcgttat cgaaaaagca ctcgaacgga
    61861 aatacaaagt caaattgact ttgattcctt tgatcgaaca atttgcagcg acagcgatta
    61921 ctgatcgata aaaattctat ttgtcataaa aggctgggat ttaaaatccc agccttttta
    61981 gtcatataat aatttctaca gcaagtaaaa agaaattcct tctaacatca ggctcaaata
    62041 tcagcttgac ctgtccaaca aatcacctat gatcctacaa tgttaggcga cttctcttct
    62101 acctgaagat atcttggagt tgcctgattt cttaagatgc ctaaccttaa ctataaaaaa
    62161 ttctaataat aaacatattt tctgatagag aaaaaag
//
LOCUS       DANKBF010000007        57044 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000007 DANKBF010000000
VERSION     DANKBF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            811..1197
                     /locus_tag="PWY89_000480"
     CDS             811..1197
                     /locus_tag="PWY89_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2065541.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1291..1482
                     /locus_tag="PWY89_000481"
     CDS             1291..1482
                     /locus_tag="PWY89_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065542.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1996..2328
                     /locus_tag="PWY89_000482"
     CDS             1996..2328
                     /locus_tag="PWY89_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2065543.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2624..2779
                     /locus_tag="PWY89_000483"
     CDS             2624..2779
                     /locus_tag="PWY89_000483"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2065544.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3055..4395
                     /locus_tag="PWY89_000484"
                     /pseudo
     CDS             3055..4395
                     /locus_tag="PWY89_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4636..6252)
                     /locus_tag="PWY89_000485"
     CDS             complement(4636..6252)
                     /locus_tag="PWY89_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2065545.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6370..6588
                     /locus_tag="PWY89_000486"
     CDS             6370..6588
                     /locus_tag="PWY89_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065546.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6783..7244
                     /locus_tag="PWY89_000487"
     CDS             6783..7244
                     /locus_tag="PWY89_000487"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2065547.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7437..7676)
                     /locus_tag="PWY89_000488"
     CDS             complement(7437..7676)
                     /locus_tag="PWY89_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065548.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7700..9223)
                     /locus_tag="PWY89_000489"
     CDS             complement(7700..9223)
                     /locus_tag="PWY89_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2065549.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9241..9363)
                     /locus_tag="PWY89_000490"
     CDS             complement(9241..9363)
                     /locus_tag="PWY89_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2065550.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9393..10376)
                     /locus_tag="PWY89_000491"
     CDS             complement(9393..10376)
                     /locus_tag="PWY89_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2065551.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10400..10549)
                     /gene="rpmG"
                     /locus_tag="PWY89_000492"
     CDS             complement(10400..10549)
                     /gene="rpmG"
                     /locus_tag="PWY89_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2065552.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10570..10947)
                     /locus_tag="PWY89_000493"
     CDS             complement(10570..10947)
                     /locus_tag="PWY89_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065553.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10979..11158)
                     /gene="rpmF"
                     /locus_tag="PWY89_000494"
     CDS             complement(10979..11158)
                     /gene="rpmF"
                     /locus_tag="PWY89_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2065554.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11173..11442)
                     /gene="rpsN"
                     /locus_tag="PWY89_000495"
     CDS             complement(11173..11442)
                     /gene="rpsN"
                     /locus_tag="PWY89_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2065555.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11965..13731)
                     /locus_tag="PWY89_000496"
     CDS             complement(11965..13731)
                     /locus_tag="PWY89_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065556.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13691..15445)
                     /locus_tag="PWY89_000497"
     CDS             complement(13691..15445)
                     /locus_tag="PWY89_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065557.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15555..16124)
                     /locus_tag="PWY89_000498"
     CDS             complement(15555..16124)
                     /locus_tag="PWY89_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL2065558.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16142..17557)
                     /locus_tag="PWY89_000499"
     CDS             complement(16142..17557)
                     /locus_tag="PWY89_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2065559.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17568..18236)
                     /locus_tag="PWY89_000500"
     CDS             complement(17568..18236)
                     /locus_tag="PWY89_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2065560.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18366..18950)
                     /locus_tag="PWY89_000501"
     CDS             complement(18366..18950)
                     /locus_tag="PWY89_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2065561.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(19281..19514)
                     /locus_tag="PWY89_000502"
     CDS             complement(19281..19514)
                     /locus_tag="PWY89_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL2065562.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19529..20479)
                     /locus_tag="PWY89_000503"
     CDS             complement(19529..20479)
                     /locus_tag="PWY89_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2065563.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20476..21318)
                     /locus_tag="PWY89_000504"
     CDS             complement(20476..21318)
                     /locus_tag="PWY89_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2065564.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(21320..22039)
                     /locus_tag="PWY89_000505"
     CDS             complement(21320..22039)
                     /locus_tag="PWY89_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2065565.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22731..22877
                     /locus_tag="PWY89_000506"
     CDS             22731..22877
                     /locus_tag="PWY89_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065566.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNT"
     gene            22949..24175
                     /locus_tag="PWY89_000507"
     CDS             22949..24175
                     /locus_tag="PWY89_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2065567.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(24263..24655)
                     /gene="rpsI"
                     /locus_tag="PWY89_000508"
     CDS             complement(24263..24655)
                     /gene="rpsI"
                     /locus_tag="PWY89_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2065568.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24671..25114)
                     /gene="rplM"
                     /locus_tag="PWY89_000509"
     CDS             complement(24671..25114)
                     /gene="rplM"
                     /locus_tag="PWY89_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2065569.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25633..26100)
                     /locus_tag="PWY89_000510"
     CDS             complement(25633..26100)
                     /locus_tag="PWY89_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2065570.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(26220..27758)
                     /locus_tag="PWY89_000511"
     CDS             complement(26220..27758)
                     /locus_tag="PWY89_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2065571.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27838..28524
                     /locus_tag="PWY89_000512"
     CDS             27838..28524
                     /locus_tag="PWY89_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2065572.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28725..29252)
                     /gene="msrA"
                     /locus_tag="PWY89_000513"
     CDS             complement(28725..29252)
                     /gene="msrA"
                     /locus_tag="PWY89_000513"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2065573.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(29336..30013)
                     /gene="pgmB"
                     /locus_tag="PWY89_000514"
     CDS             complement(29336..30013)
                     /gene="pgmB"
                     /locus_tag="PWY89_000514"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2065574.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(30010..31140)
                     /locus_tag="PWY89_000515"
     CDS             complement(30010..31140)
                     /locus_tag="PWY89_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2065575.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(31140..33323)
                     /locus_tag="PWY89_000516"
     CDS             complement(31140..33323)
                     /locus_tag="PWY89_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2065576.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(33320..34345)
                     /locus_tag="PWY89_000517"
     CDS             complement(33320..34345)
                     /locus_tag="PWY89_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2065577.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34390..35217)
                     /locus_tag="PWY89_000518"
     CDS             complement(34390..35217)
                     /locus_tag="PWY89_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2065578.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(35214..36281)
                     /locus_tag="PWY89_000519"
     CDS             complement(35214..36281)
                     /locus_tag="PWY89_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL2065579.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(36307..37143)
                     /locus_tag="PWY89_000520"
     CDS             complement(36307..37143)
                     /locus_tag="PWY89_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2065580.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(37140..38009)
                     /locus_tag="PWY89_000521"
     CDS             complement(37140..38009)
                     /locus_tag="PWY89_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2065581.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(38079..39362)
                     /locus_tag="PWY89_000522"
     CDS             complement(38079..39362)
                     /locus_tag="PWY89_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2065582.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39376..41061)
                     /locus_tag="PWY89_000523"
     CDS             complement(39376..41061)
                     /locus_tag="PWY89_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL2065583.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            41336..42361
                     /locus_tag="PWY89_000524"
     CDS             41336..42361
                     /locus_tag="PWY89_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2065584.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(42956..46630)
                     /gene="rpoC"
                     /locus_tag="PWY89_000525"
     CDS             complement(42956..46630)
                     /gene="rpoC"
                     /locus_tag="PWY89_000525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2065585.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(46688..50305)
                     /gene="rpoB"
                     /locus_tag="PWY89_000526"
     CDS             complement(46688..50305)
                     /gene="rpoB"
                     /locus_tag="PWY89_000526"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2065586.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            51033..52001
                     /locus_tag="PWY89_000527"
     CDS             51033..52001
                     /locus_tag="PWY89_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2065587.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            52019..52924
                     /locus_tag="PWY89_000528"
     CDS             52019..52924
                     /locus_tag="PWY89_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065588.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            52921..53727
                     /locus_tag="PWY89_000529"
     CDS             52921..53727
                     /locus_tag="PWY89_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2065589.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53863..54852
                     /locus_tag="PWY89_000530"
     CDS             53863..54852
                     /locus_tag="PWY89_000530"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2065590.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(55169..55693)
                     /locus_tag="PWY89_000531"
     CDS             complement(55169..55693)
                     /locus_tag="PWY89_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL2065591.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            55980..>57044
                     /gene="scm"
                     /locus_tag="PWY89_000532"
     CDS             55980..>57044
                     /gene="scm"
                     /locus_tag="PWY89_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2065592.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        17266 a        11520 c         9693 g        18565 t
ORIGIN      
        1 agtagttcta tctgagcgat actcttttat aatactgaat tcggaattaa aaaccacgat
       61 aggtaacttt aaacaaacat gaagagactt taataagact tctttatctg aaaacacttt
      121 tgtttctctc tttcctttct acatcattaa atagtatttt atacgttatc taaaatacta
      181 ttcctctgtc aaaataagct aacttacttc attataaaat atccacttat cgctttcatg
      241 tatattccac cgtaaaaagt acattttatc accgagaact catcattttt catcatgatg
      301 aatactattt acatatatct agttcaaaga caataattat tatgatccgt tattatctaa
      361 gcactataca gataatagga atttttcgtt tctaaatatt ccaatttggg aatccaataa
      421 aaattttcgt tcaaatacgt gtctattcga actcttttct agttgttaat tatgaagggg
      481 gaagaaataa aaaatacaat ctatacacga ataagaatat tatccaaaag gaggttttag
      541 aaaagattta ttatacgtaa aaagctcaag acattttctt agccataagg acatgtttga
      601 tatttaagaa gatatatgaa ttttcttttc taagtataaa gataactatc cattatcaac
      661 taaaaaaaca tttttgaaaa taaacatcag cagtcctatc aatgcatgtg agcttataga
      721 agttattcgc agcgcagcat aattgcatta gcttcttttc gtgttttttg cctcttttcc
      781 ctttaaatct taatggagga ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc
      841 cagttgattc gattctttga aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa
      901 caagaagaaa ttcaaaaaca tgaacaactt gatcaactcc caccatattt gtttactaaa
      961 gatactataa aatttaaaca accgattaat gttttaaatg tgactctcct tgttgaagat
     1021 ataaaattag caaaaatcct tcctttgttg aaagaacctg aacaaatttt tcttatcgaa
     1081 aaatttatct ttgataaaac tgataaagaa attggaaagg ttctggggat tacaagacag
     1141 ggtgcgacaa atttaaaaca tcgactttac aaaaaaattc aaaaaatgct ggtttaatat
     1201 ctgcataata actaattttg tatgcttctt tgctcgttct cctccttccc aaagaaatag
     1261 attataagtc catgggaaga aggtagattt atgaattctt taaaagaaga tttcatttta
     1321 gcaaaggctg gaaatgaaga agctgttgag gcaattctca aaagatttag ctctttgatt
     1381 cataagcaat cctggagaac tggaaaatat gatcaagatt gttatcaaga atgtatgctt
     1441 gcaatatatc tagctatctc gaaattcgaa attaaagaat aactcaacta agggagtctg
     1501 tgacataagt aaaaagatag tttcaaattt cgaacaataa atttgaaact atcttcttta
     1561 taattataaa gtatcgattt tacagcgttc tataatgatt catagaatct aaaacgaatg
     1621 attttaatta tcaacgtttt ttaattcgtt tttagcttaa aaataacttt tgtcacagtc
     1681 tcactcctaa aattacttta tttacgtatg aagtgggcag gaggtcgaat ccagaactgg
     1741 gctatggtac ttcaacgagg acttctcctc tagaattgaa aagcatgcga tttaccttcc
     1801 gtaacgacgg tagatttatt caactatgtg tccaatcaaa atttgtctga tttcaaaaat
     1861 agtcaagaga attttggttg ttaaaaactc ttatctatca acttccacag tttagttgac
     1921 aaaccctgat actaagttgg gctatgatac acttttcttg actatcagtt acgttaccgg
     1981 aaggaatgaa ctctattgag aaaaacatat gatcgtaaat ttaaactaaa gatttcacag
     2041 gatatccttg agaaaaaagt aactaccaag aaaattgctg aagaatacaa tatctctcgt
     2101 ccaacaattt cccgatgggt ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga
     2161 caaggaaaaa gattacctga taaagcagat ttctacattc ttgaacagga aaataaacga
     2221 ctaaaagagg aagacgagtg ttctacgaac acaaagatcg atacggatct ccagggatca
     2281 ctcaaaaact ttacgatgaa ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc
     2341 gtgaaatgaa tttatgcaca agaggatttc atcgacgcaa atcttctcat ggtagagaaa
     2401 aaacaatcaa agaaatggta aaaaaatgtt gaatcgataa tttgaacaga accaaattga
     2461 tgctgtttgg gtgatggata ttacttacat tccatgtaat gacggtcgcc tctatttgtc
     2521 cacctatatt gacttagcaa ctcgtattct acgttactat atggtagatt cacacatgaa
     2581 aaaagagatt gtcgtccgtc cattacaaat ttattaagga gaattgccta cggtaattca
     2641 ttccgataga ggtagccagc atcggtcttt tatttatcaa gagctgttga acgaccacca
     2701 catcacacat tcagtgagcc aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt
     2761 tcatcgatca atcaaatgag aattaatcga ctccaacaaa tataaaagta aaatagagat
     2821 gaaggtgtta atccaagatt atttagaaga ctactaccca aacaaaagaa tccatacaaa
     2881 atttatgatg actccttgac aatttgaagc gaagaaaata agggatctat aatatctagt
     2941 gttggtgacg ccttctgttg ccaatacttt ttcttttttc taataaacgc aaacagacac
     3001 cttaaaatcc tttagaataa agtcgacgaa aaccattcaa aggagtgttt caagatgtcc
     3061 tattcagagt ttaccaaaga gttactagat attctagacc taaatcttat ctttcatgaa
     3121 gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga tggcacattg
     3181 acctatcaac cggaggaatg ctttcatcgc cactatcaaa atgaacaaac gatcattaaa
     3241 tagggctgga aaaaagtatc catcttattg aatgatgtca gtaactacaa gaccattctg
     3301 cggattaaca aacagcgctt caaatgtaaa cactgtggaa agactttttt agcagaagat
     3361 tcagttagtg atcgtcgctg ctccattgct cgcagagtca aacaagccat tcttgagcta
     3421 ttaagcgaac ctctttctat gtctttgatc gcacgtatga agcacatttc tccgaccact
     3481 gtcattcgaa tacttcgcag ccttcggcct aaaaccattt ctttgagcca gctgttacca
     3541 gaagtggtgt gtttcgatga attcaaatca gtgaaaaatg tctcgggagc catgagcttt
     3601 gtcatgatgg acggcaagac ccatcaactg attgatattg tggaaaatcg gcaactcaat
     3661 cctttacgcg actatttctt gcgctaccct ataaaagtcc gtgaacaagt gcgtctagtg
     3721 gtttcagatt tctatacacc ctatcgcaca ttggtgaaag agtgttttcc gaatgcccga
     3781 atcgtcgccg atcgctttca tattagccaa cacattggtc gggcttttac caaccaccga
     3841 attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt cgaagcactt aaagaaatac
     3901 tggaaactac ttcaaaagaa tgcgtgggaa ttgaaaggtc agcatcgcta ctggcgtccg
     3961 tcatttcgag atcatctgac agaagcagag atagtcgatc ggctattatc ttatgatgat
     4021 tccttaaagc gaggctacga agtctaccag gattttcttt ccgttatcag aagacaagat
     4081 gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg agttgccaga gcactaccaa
     4141 ccagtgttca cgaccttcaa gaaataccaa acagaaatca aacgagcgtt gcgcgttccc
     4201 tattccaacg gccctataga atgtttgaat aaccatatca aagtactaaa acgaattgcc
     4261 tatggctata gaaactttca aaactaccga gaacgaatct ttttatatcg aggaaaatat
     4321 tttaagaaaa ctaagaatac tacccaactg actaaagcca gaacaacaac acggctagac
     4381 aagatagctg tctagccgtg tgagtatctc gattattcta ttgaatttct tcaccaacac
     4441 tcattgacga agagccaaaa taagtaatct aaccgactgt taactaaacc attgcaggtc
     4501 ccgttaaaga tacttgtgcc tgttgtcacc gcttcttttt cttgtccaca agcgcttaac
     4561 acacttaata aagcccctgc tattattaat aataatatct ttttcttatt aaaaacctcc
     4621 tcatctattt ccagattagt aaatgtagta atctgtgctt tcttttttct caatactaat
     4681 aactaactta tggggtaaac aaatactcgt ttgaccgggt tttgagatcc atccagttct
     4741 aaccgcgatt tgatcaggac tattatcttc tttaactcga attcttccat ctttaatctc
     4801 aacaatatta tattgtccct tcgcaggatg ataattcacg agtttatgat tgatcttatc
     4861 caaatcgaag cgatccattt ccttgccatc aattcgaacg atggcatatt tgatagcatc
     4921 ttcagttgtt tcattctcct tttctataga gaatacaaat aagggaataa aagaggtaat
     4981 aatcaaaatg ccaataatta tgacatcgcc tatcttgaca ccagcttttc ttaaaaattt
     5041 cattaacgat atctggctaa acatattaga ctttttccat ggaaatggtg aaatttttcg
     5101 gaaattatct gcattaagag aatcagttaa acaaccctca agttgttcct taatacattg
     5161 ttgatccatc ttcataccaa taaaaactag tttattaatg cgttcaccaa acaacggatg
     5221 ccaggcttcc ttcaattcgg gatttttttg tataatcatt tgacactcaa ttttaggtaa
     5281 actagctacc caataattta atgattcaat acttttatag ctacccgatg tttcaaagat
     5341 cgtcgaaaaa tcactatgtt gggcaaacca aagaaaccct tttgctcttg taatattgcc
     5401 aggaaaattt ttatccgtaa gatgggcaaa tttttgtgcg tcaaggagta accgatttcg
     5461 atagataaaa cttgagatac catattcttc tgtttcagga atatgatttt gatagccaag
     5521 ctctaactct ttgaaccagc ccactgaata ggaagtctca tctatattaa aaagatttgt
     5581 atttagaatt ttcgataagt ctacttcacc tttaattgtc ctaattattt tggcattgga
     5641 ctgtaactta gcaattagac cgttaatttt gtaaatcgtc ttggttgaca ctaagtcact
     5701 attatttaaa agtaatatgt tacaacactc gaattgatca attaacaagc cacgaatatc
     5761 ctgttcttct tcaactacat taccttcagc ttttctttcc tgaatctttt cacccgtttc
     5821 gaaattatcc caaaaacgtt ttacatccac tactataata atggcaccaa tctctaatcg
     5881 ttgggtcaaa tcaatgtcta aagggttttt agctaatata attgcttgaa taattggaat
     5941 tggtttgctg attcctgaag cttcaataac aatttgattc aagtcagaaa attgcactaa
     6001 ttgatctagt tcaacaatca aatcttcgcg taatgtacaa caaatgcatc cattcggcat
     6061 cgaaacaagt ttttctccag ttcttttaaa acctttcttg tcgactaata ccccatcaac
     6121 atttacttcc cctatgtcat ttacaatgac agcaatttcc tttccgtgat caccgtacag
     6181 aatatgattt agaagtatcg tttttcctga accaagatag ccagttaaga ttgtaatagg
     6241 tatttttttc atagggaacc tctttctata ccttgaattt acactttaag tgcaacacat
     6301 aaaaatgagt cataatcgat atccaactag tataagtttg tcgagaacac tcctagaagg
     6361 aaaataatta tgagccagta tcgtcatctt actatactcg aacgagaaag aatctttctg
     6421 cttcacgaag aaggattttc tattagtata attggcaacg acattcagca tagcccgttt
     6481 actattttac atgaactgag aagaaacaag aaaaaaacta ttcaccttcc aaagcggaaa
     6541 aaaattatgc ttatcgaaga aaaaaatgcg gacgttatca cattctaaat gatccgtgag
     6601 tttggtcaat tgttcgtcat ttatttgtcg attttcagtg gtcgccagag gaaatctcga
     6661 atcgtttgaa gcatgaacat atggactttt caattagttt tatcacgatc taacgtgcaa
     6721 tttgcaatga tcttttcgat acagaacccc tctcacacgg caataaaggc ctgatccgtt
     6781 cattgcacca tcgaggaaaa acaagacata caaaaaatca tgtcgagtct cgtggaaaga
     6841 tttgccttac tcattcgatc catggccgtc caaaatccgc agatgataga agtgattttg
     6901 gtcattggaa agccgatatt gtagctggca aaactggtgg tacttgttta gtgactttga
     6961 cagacagaaa atcatgcttc cttttagcag gaaagaaggt ccccagaaag aaagccgctt
     7021 atgtgagcga gaaaatgatt gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga
     7081 tcactccaga tagaggaaaa gaattctcca atcatttcgc agttacagaa gcattgaata
     7141 atatgccctt ttattttcca gatcctcatg cccttggcaa caatgaacaa attaaaatac
     7201 gaatagactc attcgagaat acttgccaaa atcgaaagag atagctacgg tagataactc
     7261 aacgataaat cagatgattg aaaaactgaa taacagacct agaaagtgtt tagattggag
     7321 aacaccttat gaagtcttct ttgataaagt gttgcgcttg atttgacaat tcaagacata
     7381 ataaaaagca gatcaaatcc aaataaggga aaaatgatct actctggtat tatcaattaa
     7441 ttggccaagc tattatgccg ttttgctaaa cgtctacttc ttccagtatt gaccttgata
     7501 aaacaacaat caggatcccg ttttaatact ctatgaactg tgtcaatact aacatgtaaa
     7561 gttgcagcaa tatcagctaa tgtactccca tttaatctca tttgctttgc aaatcgataa
     7621 cgccgataag catcaatatt cttggaatcc atagacaata atacttttct taccatgtat
     7681 ttcctccaaa aattgaattt taatgagcca tcatttcttg agcaatttca tgtttagaca
     7741 atgaagctgg atagaaagta ggccaattat gcaattcatt atagagtttt tcttgacttt
     7801 ctccaccaaa gaaaatatgg aaatgctctg ccttacttgg tgcaattcca tgatcactaa
     7861 attgaatata tttaaaacga ccagcacctg catcatcagt ttcaaaatta aaacgaacac
     7921 cacggtttcc tttttcataa ttcaaaattt tgtatccaac atatttgtat gtgtattgat
     7981 gatgttcacc gttgactaca aaatcaatgg tgttgtcttt aatgttgata gtatcaatat
     8041 ctgtcttata gccagtagta taataatctt tgtactcggc agctgtcatg tctttattga
     8101 tttttgcttt gtaatcgaaa acttcatcca aaattccatc ttttaacaac ggataaacag
     8161 attgccattc gcctgtataa tcactcaatt cgcgatcctt cacatcggca tctttaaagt
     8221 acccattagc aactgtcttt tcatccttcg cttctttttc tggaactacc tcttcaccag
     8281 caaccatagt tgttttttct aacgctttta gattgtcttc cattactgaa acataatctt
     8341 caccattatc catttgttct tttgtaagac tttcaagagg attcagtact tccaatttta
     8401 tcccagcttc gttggccaaa gtttttgcaa ttttatcatt agcattcttt tcaaagtaaa
     8461 tataattgat cttatttttt ttaacatact cttttaattc agctaatcga ccagaagatg
     8521 gctcttcttc aggacttagt ccagcaatag gaacttgttt caatccataa tccaaagcta
     8581 aatatccaaa ggctgcgtgt tgcgtcacaa aacttttttg tttcgcttct ttaagactag
     8641 tggtatagtc agcatccaac cgtttcagtt tagtcaaata cttttcagca tttgtctcaa
     8701 atacttttgc ttttttcgga tacaatttaa ctaactgatc cttgatattt gaaacttcct
     8761 taattgccat tttgggtgaa acccacgtat gaggatctag ttcatgatgg tgatcttctt
     8821 ctccgtgatc atgatcttct tcttcactac ctggcaataa aatcatgtct tttgttcctt
     8881 ctactacatt aggcccagct ttcttccaac tttctttagc ttttggtacc catgattcca
     8941 tgttttcgtt atgatataca aaaacatcag catcatgagc ttttgccata tccttagctg
     9001 agggctcata gtcatgaggt tctgatcccg ctggaaccat taaatccacg ttcgcttcat
     9061 cgccgacgat gtttttggtg aaatcataca ttggatagaa tgttgttaca atttctaatt
     9121 ttccatcttt atcattcgat acttttgcat cattagtgct ggagcaagct cccaataatc
     9181 ctagagctgc tatcccaata agtcctacac tgattttctt cattcttttt ccttccttaa
     9241 ttatgctaat ttcttcattt tatttcgttt atgttctttg ataattttta atgccctttt
     9301 tctagtttta atattcgggc ttttaagtcg tcgatatgca ctagatatat ccgttttctc
     9361 cattagaaat tacccccaat cactacttat cattatttgc tttccgccag taatttatca
     9421 aaactctgtt tgatcctttc ttcatccaac atttttccga ttagaataat agtggtttgg
     9481 tctttttgtc gagtggtgta ttgaaaatta acctgttcgt ttacctcttg catggcaact
     9541 ataaaatcct gctcttgtaa agcaatcaat cctttaaaac gatacaggct cccttggttg
     9601 gttaaaatca gccagtcaat ccattggagt aaaaaatctt tttttatgtc ttgatctgaa
     9661 gttaagagaa ttgatttaaa ctcatgtact tgatggtgtt gatgatagta tttgccatta
     9721 tagtgatttt ctgatgaatc actttcaaaa actaagggat gattaaattt tttcaaatta
     9781 taaaaatcct ttttttgaat cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg
     9841 ttaatacttt tgatttctct ctgtaatttt tttagtgcag ataaattcaa gttttgtttt
     9901 ctagacacaa aaatgcgatc tgccatgaca agttgcttaa gggcttgtgg ttctctttca
     9961 ataagttcaa aactgtcact atcaataaca gtcaataaac tatcgatata tactcttcct
    10021 cttagttgag gcgctgttaa aagtgtttga atgattggtt ggggatctgc tatacctgta
    10081 gtttcaatga tgatttgtga aattggttgt tgatgttctt ctattgtttc cagtaaagaa
    10141 atcaatattg ctaataaatc actgcgaaga ctgcaacaca tacatccatt atttagttgt
    10201 aacacccgtt cctcgctatg aactaataat ttgtgatcaa tcccaacttc accaaactca
    10261 ttttcaatta taataatttc ttccctgggg tagggtgaga cttgcaaggc ttgattgatt
    10321 agcgttgttt ttccagcccc taaaaaccca gaaacgattg taactggtat gcccattttg
    10381 tcaccttttt accacttagc tatttcactt ctttaaaaat tgcccgtctg cgcaattttg
    10441 gcgaatactt ttttaattct aaccgttcag ggttattccg tttatttttg ctggtgagat
    10501 acagacgttc tccagtttcg acacattcca aaataatatt ttgacgcata aatcttcctc
    10561 cttcaatact tatcgatctt caagatatgc ttgaatttgt tgtgcatctt caaaagttaa
    10621 ctcgccagat tccaattgtg aaactaaatt ttctttcata tcaacaggta tctcccaata
    10681 accatcgata cattgaaata actcctcgtc atactcttca aaaattggat agattcttaa
    10741 aaaatcatca aatgataagc cttggtcctc atagtaatct ttgactaatc cattttcaat
    10801 tgcctgttgg gtcggtaaac ccgtttcagt aataattcct ctctctacca tcgaacgata
    10861 aaaagtgcat ttatctagcg attgctgggt cgtttcaaga taatacttta atccttcttc
    10921 aactaaaggt gggtaaataa tttccatttt gattccctcc taaaagttat atattaaact
    10981 attttgaagc tcctttaatt actttctttc cattatagta cccttttaaa gatacatgat
    11041 ggctacgtct ataatcacca ttcgcttcgt caaatgaaat ttctggtttt actacagttt
    11101 gatgagttct tcttaaccgt ttcttcgcct tagatgtttt tcttgctggt actgccatga
    11161 tatttcctcc ttttaccaac ttgctttttt tactccagga atcaaccctt gatgggcaag
    11221 ttctctaaac ttaatacggg acataccgaa ctttcgcata taccctcttg gacgaccatc
    11281 cgtctgatca cgaagcctca gacgattcgg atttgaatct cttggcaatt tagcgagagc
    11341 ggtgcgatct cctgcggctt ttaattcctg acgtttatct gcatactttt caatcatcgc
    11401 catttgtttc tttgctttag caattttcga cttttttgcc atataccttt atactccttt
    11461 tattttgatt tagacacctc aaatcgtaat cgttacgttt tgacactgat actttaccaa
    11521 caagagttgc tcttgtcaac aataaataac atgaaataaa actcatatga atagataact
    11581 atttgaaaac gaatttatcg gtacatagag ataatcaaat catttaaaag aatatgcagt
    11641 ttaggctctg aaaaataaga attcactttt attagtccaa tcaaacacaa ataccgtttt
    11701 aagtagcttt tcctctactt aaaacggcaa tatgaaagaa actataactt tttattcaat
    11761 cttttatgtt taactggttt atcgccaatt ttttaaaact ttgattattc cttatattat
    11821 aagaatagac attaagtgaa ctaattcaat catactgatt ctttcgtgta ttcaaaatta
    11881 gatgctgttt aataaaatta ttctttttga aaaagaatta taactatatc ccaaaaattg
    11941 taggttaatt taaagcctat ttcatcatcg tgattgagct tctttttcat agacgaattt
    12001 ctcagatagt ttagttttct ttatgagatt tttgtatatt tctgaccttc gatacaactc
    12061 attatgagta ccttgatcaa ctagttttcc ttgatccatc actacaattt tatttacctt
    12121 ttcaatagac ttcaagcgat gcgatataat tagtactgtc ttatcttgta ttaaacgatt
    12181 taaactttcc tgaatacgtt tttcattttc cgcatctaaa ctggcagtaa tttcatctaa
    12241 aattaaaatt ggcgcatttt tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc
    12301 tccagaaagt gaacttccgt tttctccgac caatgtatga taaccgtcag gtagtttttc
    12361 aataaagtct tcgcagttag ctaaacgagc tgcttttttc acttcctcat ctgacgcttg
    12421 actatttcca atacgtatat tttccaaaat acttgtatta aacaaagtta cctcttgaaa
    12481 aacaatagcg attttgctaa acaaagaagc tggagataca cgtttaatat catacccatc
    12541 aattaaaata cagccttcat cataatcata cagtctagac actaatttca agatactagt
    12601 tttcccggaa ccactagcac ctactaacgc agtcacttct ccttgtttag ccgtaaaaga
    12661 gatatgatct aaaattgggg tattgttatc atatgagaaa gagacatctc gcaactcaat
    12721 gtcaaaagat ttcagaggac tatctgatcc ctcttggata ctggtttcct tcatagctct
    12781 aattcgctgt attttaggag ctaaataaaa tatttcaagc actgcttctt tcatagaatc
    12841 gaaggaatcc tttattttta ttgcggctag taagtaacca acgacgtaga gtatagtcac
    12901 ttcacccgtt aacagtaagt gaacacctac tagaatgact actgctaaac taacatatga
    12961 gaaaatagag gataaggcca tgactgaaaa agtacttaat tctaccttta aatgaatcct
    13021 ttcactctct tccatttttt tgaaaagtct gtcttgaact tttttagata gattaaagct
    13081 attaatctct tgctgcaatt ctatagtttc ttgaaattct tccgagtttt ctctcaaagt
    13141 atcgtaatat ttagtattgg ctttcgtttg ggatttcttt gataacaaga ttaagacaaa
    13201 actaaataac gttggcaaaa taacagctaa tcccattttg acattaccaa ccaaaagcat
    13261 cactgaaata aaagggaaaa acagagccat accgccggct ttaggtattg catgactcat
    13321 cgcatgctca ataccttcaa cgtcagacat gatagtttga gataaatctg ataaattatg
    13381 ttttgaaaaa taggataatg gtaaatttga caaatcgtct gcaatttgca ctcttaaatg
    13441 cgcactttct ttataagtac tattatataa gttttcatac tccctatcta acaaccaata
    13501 catgaaaagc aaagtaacaa acgatacggc aaaataaagc caacggggct ttgtattttc
    13561 taaaactaat tcgtctatca gcatcattat aatgaatgct ggtaccatat taatgcagta
    13621 tacaaaaaaa ctagaaatag aagctttagt taaagcttct gcgccctgat cacttaaagc
    13681 aaatttgttt ttataaaaac ttttcattgt caagcctcca atcatttgca ttctcataat
    13741 tagtgaccaa ttgtttatac acttgatgtt tagccattaa ttcattatga tttccacgtt
    13801 ctactatttt cccttctgac aagacaagaa tttcatctac agatttaatg ctggataatc
    13861 gatgagctat cataataact gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt
    13921 catactcatt atctggatca atagatgcac tggcttcatc cataataaca atctttgcat
    13981 cttttaaaat tgctctggca atagctatac gttgtttttc tcctcctgac agataaactc
    14041 ctttactacc aattaaggta tgctctcttt cgggaaattt ttctaaaatc gattcgcaac
    14101 cggctaatct tagcgcgtca aatactttac ttgctggcgc ttgaggattt gctatagaaa
    14161 cgttatcgta aatactctga ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt
    14221 taataattgt gtcttttgaa tagtgattta aaggcttacc accaatttta atctgtccct
    14281 tatccacatt gtagaaacct gaaattagtt tagctatcgt actctttccg cttcctgatt
    14341 ttcctactaa tgcataaatt cttttttgtt ctaaaactaa gttgaaattt tctaaaacac
    14401 actcttcttt gtatgaaaag ctgacatttt caaatgcaat gtcacaattt ttaatctgtg
    14461 tctcgttccc atattttagt tggtcttgct tcatttctaa ataaagggag tctagtgttt
    14521 cgactgcata tcttgcttca aacaagtgca tacttacata cattattctc atgaaagaaa
    14581 caaaaataat tccacttaaa aaaaacaaca tcaacaagtc aacagctaat atcttgggag
    14641 tggtaagaga agttataaaa aaggttatag gaactatcaa aatgcaaact atgccaaaaa
    14701 atagccattg aaaaagaaca aaaggtgttt tcccactcaa agaatactta tatgcaaact
    14761 tagcataatc gttaattgct ttattcaagg ctttaaatga tgttacttta gcaccaaata
    14821 tctttataac ttgaattccg cggatatatt ctaccgtttc agagcttaac acatctaacg
    14881 attcttgata tttttgaata aaatttgtat tctctgtcat tttctttaac aagaatacac
    14941 caattactaa catggcgatt agaaccaatc caattttaaa gttaatgaaa aagcctaagg
    15001 caactgataa aacaggtgtt actatggctt gagcactatc tggaatcaag tgagcaattg
    15061 ccatatgtgt tttagccgcg ttgtcatcaa ttgtctttcg aatatatccc gaagaattta
    15121 aatcaaaaaa acgaaaactt gcttctgtta aaccgctaat tccctttttt ctcaaattag
    15181 tctctaagcg aaacccaagg atatgagaaa ataagccaga aaaaatataa aataaagctc
    15241 ctaaagttaa ccaagcaaca gtttgaaccg cataactctt agctaaatat tcatctccta
    15301 caataattag agactgaaga aatttgtaaa tactgtaata tccatagact gttaatacga
    15361 cagaaaaatc agaaaataaa gtggctaacc atccgtagta tttcatttta gggacataac
    15421 taaataattt tttgtatact ttcatttttt cctcccccgc attcaatttc ttctcttatt
    15481 cgtttacaac tgttaatgct ttccgtaata gcgtaaatta gagatgtaca aacagataaa
    15541 aggtaaacta cgttctattt tttgtatttt tttaagactt ttcttccaat caatacacca
    15601 atcatagata aacttgctac aactaacact gaaatgattc cccatatacc taccgcatag
    15661 tggatcattg catccattgt tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa
    15721 cgatctgtaa agaaccatat aggaatccac tgtccggcag cactgcctaa ttgcattaga
    15781 gcccaagacg caacttgagg aattgctcga tcatatccaa atacagatag aataatatca
    15841 gctaagatcg ccatactaat cgaactgata aaccaaggta agtacgcccc gcccattaac
    15901 gtaaaaaata acaataaaac aattgtgtaa atagtaaaaa ttccttttct agggtagtta
    15961 tagctaatat aaacaaatat gattccagat aataatccaa aaatcccggg ggatatgctg
    16021 tgaccaaacg ccccaccaat actactaacc attattgaaa aaaagtaaat tactagggaa
    16081 ataactgtag ctaaaccaat aaaaatataa tcttttgttt tcatatatat tctcctcaat
    16141 tctatttcgt tatgtcatta aaaatctgtt ctatcaaagt tgaatcttct actctaagtt
    16201 ttatatcttt ttttattcca ccatttttta aataaataat tctattgcat gttttataaa
    16261 gaaattccgg atcatgagta gctataacta ctgcaccctt ttcttttaag tctttaataa
    16321 ggtcagcaat cctttccata ttaatataat ccaacccact tgtaggttca tcaaatagaa
    16381 ataaattagt ttttgacaat gaagctatag aaaccaataa tctttgtttt tctccaccgg
    16441 ataaatcaaa cggtaattga tctttcattt tataaagttc catttttttt aaaatttttt
    16501 cttgctgatc tttactaact atttcattcc ccagggctaa ctcgctcctc actgattctg
    16561 tgaaaaattg gtaatccatt tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat
    16621 tttcgattat ctttcctttg gtagggcgaa taatcttcat gagtattctt agtaaggttg
    16681 ttttccccac accgtttttc cctaccaaac caataacatc tccaactttg acatcaaaat
    16741 ttatgtcttt tagaatattt tcataagtaa tttcttcaac ttttaataca tctgcactta
    16801 ccttttcgat atagggaact tcaatcgaac tattaaaaat gtcaaatttc cttaatgcgt
    16861 tattgttttc agtttttaac aactgaatag ttgtagtttt tacagttcta tttttaacaa
    16921 atagaacttg atcaattaat tctttcaaat agtaaaaacg atgttcagct ataacaatag
    16981 taaatccttt attttttaaa tcacaaagta agtcagcaag tttcttagtc gctttgaagt
    17041 ccaaatttga agacggttca tccaatataa ttaatttaga gtttagcgat aaactacatc
    17101 caatagaaat tttctgccgt tcaccacttg atagcgaaaa aatgttttta tctaatatat
    17161 ttgaaagatc taataattca gctaattcat tgattctttt atccatatgc tcttttgtaa
    17221 atccaaagtt ttccatcgaa aatactaact cactcgttgt atttgttgta aagaattgtc
    17281 ctctaggatc ttgacttacg tagccaatat tttttgatat actttcttgt gttaaggatt
    17341 gtagcgagtt attttcaaat aaaatttccc caaccaattt tccgtcataa agtcttggaa
    17401 taagtccatt cattaacttt aatagtgtgg atttcccact cccagaagaa ccagtaatca
    17461 atgtaagact gccttttttt attgaaaaat taaggtcaac aagcgcttgt tccttaacat
    17521 ccttatatga aaaagaaaca ttgttaaatt caaacaattc tttcacctca aatcaaaata
    17581 cttaagaaaa aaatagtaac ggaaaacagg atgatatagt ctaataagct catgtttgtt
    17641 ttatagtaag aacttctttt cacaggagat tctatcccct ttgttattgc attagcggtc
    17701 atttcttcag caatcatagt tgcacgtata agaacgggaa ctaacagaaa ttccaaatat
    17761 tgcagaggct gaaaaaaaga caagcgaatg tttctcattt ttaaagattc tttaatcatt
    17821 cttatttcag aacgataagt cggaaagaat ctgatcacta ctgtaatact taacattatt
    17881 tttttcggta aatgtatttt ttctaaagag gataataatt cattgctagt atagacacta
    17941 accaacaacc atgagatcat tacgaccggc aacattcgaa atacaatata taaggatgtc
    18001 gcaaaaaata tagtaaaaaa ctctatcttg atagcatcaa taattttcat tactaaaaac
    18061 agaactgaat aaatcaagat gaaataaata catgtcttta ttttacgaaa catcaatact
    18121 aaaactgtag caataatgaa acaccccact tgaaatatat ttccctttcc taaaaaaata
    18181 atgaccggaa aaaaaatatt taaagtaatc aaggttaacg gattgatttt tgacactctt
    18241 tcacctccaa aaaataagat cgcactgtta tcttaagctg ataaaaatgc accgttattc
    18301 agataacagt gcgatcttat caaaaagcat aactgagaac aattctcaat gcaagctttt
    18361 tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa tcttctggtt
    18421 aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa tgtagcgtag
    18481 aatgagtaaa tgtagaagtg gatttcttct tcattaaaga ctaaattttg attgatcatt
    18541 ccgatagtct ttaacttatc tagacaacag actgtctttt tcgtcacaaa tgatgtaatt
    18601 ttttcaacaa tatcttgata tttggttcca gctgattgga ataaaatcaa atcgaatata
    18661 cgtttattct caaaaatata attaataaat ggatctaatt cattgaagtt ctctctccaa
    18721 cttttctcta tatctatatt ttctaagttt aagaggtatt tggtaaactc ttcattatac
    18781 atattaataa gcgcattaat atgcggctga ataatttctt caaataacgc ttccttagaa
    18841 gtaaaatgtt tataaaaagc acccgtagtt acgcctgcgg tcaggcacag ttgccttatc
    18901 ttagtttgct gaaatccttc tttttcaaaa tggcttagtc cactttctat taacttttgg
    18961 tgagttataa tataatctct ggtttctatg ggtacttcct cctaataaga tgaattaact
    19021 ttattatctt acacaggact tgaagtttca atcgttaacg tttattactg aactcattcc
    19081 ttagagctaa actaatgaga aagagcagaa taactgatac aaaaagcaat cctacaagaa
    19141 ttaatagact catctaaatc attcaccttt tctaaaataa tccgagaaag atttaaaaaa
    19201 attatatcca gctattagga taagtcctat cataatcact ccaatagcca ttttcttatc
    19261 ctccctaaaa ataaagtctt ttacttaaaa ccttttttgt aatcgtcaat aaattttttc
    19321 cctttatcag ttaattgaaa tcctatatct aaatagtcct catatctaag tttagccagc
    19381 atctcactta cagatgctgg actaacactt agaacttttg ctaaatcatt attttttaat
    19441 ttcttttttc tttttgataa ttcctgaatt gctagtaaat aaattagtat agtagtcgtt
    19501 ttaatttgta ccatacctac cctccaaatt atttcgaaag gccttcagca attttatcca
    19561 ggttccattt catcatcgca tagtaactat caccatcttg tcctttttct gcaattgaat
    19621 cagtaaaaat cgttgaatag ataggaatat tggtatcttt tgatactgtt ttcattggtc
    19681 tatcatccac actgctttct acgaataagg agggaacttt tgttgtgcgt aatttttcaa
    19741 ctaagtgttt tatttgatct ggtgttcctt cttcttcagt gttgatttcc caaatgtaag
    19801 cagaaggcac attatacgct ttcgagaaat atttaaagca tccttcactt gtaacaatca
    19861 tttttttatc attcggaatt gaagcaaatt tagatttagc ttctttgtct agagaatcca
    19921 atttttcaat atacttatct agattttctt tatagaattt tttattatca ggatcttttt
    19981 ccgctaactg tttttcaata tttttagcgt aaataatacc gttttctaaa tttaaccaag
    20041 catgaggatc ttccttccct ttctcactct gaccctctaa gtaaataaca tctatgccat
    20101 cacttgctgc aaaatagtct ttgttttcct ctttgttcgc atttttaact aattttgtaa
    20161 accaagcatt tcctccagtt tccaagttaa caccgttata aaaaatcaaa tctgcttttg
    20221 aagttttttg aacatcttca ggcaaaggtt catattcgtg gggatctttc ccaataggta
    20281 cgatactgtg taaatcaatt ttatcttttg ctatattttc ggtaatatct gctaaaatcg
    20341 aattagtaac tactactttt aatttttcgt tcgaagccac tttatctttg tctttactat
    20401 ttattgaaca tgccccgaat ataaaaagag caatcaaaat ccccagactt cccagtactg
    20461 ctttccattt tctcatcata acgatctctc cttctttctt aaaaattttt gttttggtga
    20521 gattagaaaa ctaattacaa ataagaatgc cgctgttaaa acaatacttg atcctgctgc
    20581 tatgttaaaa ctataaccaa taaacaatcc aagcaatgat gaacttgctc cgactgttgc
    20641 agaaataaat atcatagttt tcaagctatt tgcatataaa tatgctgtag ctgctggtgt
    20701 aattaacatt gcaacaatta atatagttcc cacactttgc atagctgtta ctgctactaa
    20761 agttaataga accatcaata gataatgata aaaactgaca ttcatcccta ttgcttttgc
    20821 cattaatgga tcaaaagaag tgattaataa ttctttaaaa aatagaacta ctataaccaa
    20881 cactaaaacc gaaacgccaa ttgtaatcca cttatcaata tcttgtacag ctaaaatatt
    20941 tccaaataaa atatgaaata aatccgttga gctatttgca actcctataa ggataactcc
    21001 tagtgctaaa aaagaactga aagtaatacc aattgctgta tctcctttta ttacactatt
    21061 gctttttata aaagtaataa tgatagaagc taatagacca aatacaatcg caccaataaa
    21121 aaaattaata cctaaaatat aagaaattgc tactcctggc aaaacagcat gagaaatagc
    21181 atcacccata agagacattc ctcttaaaat aataaaacaa cctactgttc cagaaacaat
    21241 ccctataact acagatgtaa ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt
    21301 aataaaattt tgaagcatat tattccccct ctccaataaa tatagtatct ccataggctt
    21361 tttttaaatt ttcttcatta aatacagatt ctacacttcc gtgagcaatt agatttcgat
    21421 tcaaaagaat aatattatca aaatacttct ttaccttgct taaatcatga tgcacaatta
    21481 gcactgtttt tccctgatgt ttcaattttt ttaacgtatc cataattatt cgttcactaa
    21541 ctaagtctat ccctacaaat ggttcatcta aaaatatgaa atccgcattt tgagcaagac
    21601 atcttgctaa aagtacacgt tgaaattgtc ctccagataa ttctcctatt tgatgattag
    21661 aatattttcc catatcgacc ttttccaaag cctttgaaac tctttgccat tctgcatttt
    21721 taattctttg aaaaactttc attccggcat atgttcccat agatacacat tcttttactt
    21781 ttattggaaa agtaaaatca atatcagctt tttgctctac atatgcaatc ttcaccagtt
    21841 ttttattgat atcttcatta ttaatggaga cctctccctg atgagggata atatttaaca
    21901 ttgctttaag caatgtggat tttccagcac catttggacc aataatacct gttaaagcac
    21961 cttcttgtat ttttaaagaa atctcgttca atgctaaaaa atcgttataa caaacattaa
    22021 gctttttcac ttctaacata actttctcct tctaaatgat aatgattatc acgatcatat
    22081 ggtatacaaa aattatattt tagtcaaccc taaattttat agtttagatt tttttctgtt
    22141 gctgaagatt agaatgacaa aactatttga aactacgcgt atagtaagta gctttcaaaa
    22201 tatataaaaa aggagcgttc tcaaatgaga acatgctcct aaaactactt tattggcgta
    22261 tggaaaaatt atacttgaag tggatagcgg gttgaataag ttgataagca tcaaaacatt
    22321 tataaaaatt attcaagact atgtctgttc catatacaaa tatgaaaatc agtaaactcc
    22381 atttaacttt tttcgtagtg tgtcttttta tacatgttta tcgttattaa tagtgaatag
    22441 gaaaaaaact atttttgatc attgacaatt aaatacaatt tttgaataac gatccctgct
    22501 attcgagcta gaaactcaag tacgccatga cctgattagc gacaaatact tagagatttc
    22561 tttttgttct gggaaaacaa aatgccatga caatagcagc aaataatgat acttccgtaa
    22621 ggctcgccgt aaactgggga gaggtgaaat tcctatgtgc ttacccaaag caattcaaaa
    22681 atttcataac tactatttta ggagatgatt ttatagaatc cagagtctac ctgaataaaa
    22741 aacaaacctg tgcatttcta ggaatcgata ataatactct agataagtgg attcgtatat
    22801 ataatttccc agtaataaga gttggtggtg tagttcgctt tgacgtagaa gatattaaag
    22861 actttatgaa tacttgaaat tttcttctcg ccgcatatac taaaattagt gaaatatact
    22921 gccttacttt ttttaaggag tgaggatttt gcctacaaag ctttcaaatg ggaaatataa
    22981 aactaatctg agatatccaa aaaggtttaa agaaataaca ggaattgcat cagaaaagta
    23041 tcaaaaaaca tttccaaata ggcaattggc gattaaagcc gaaaatgata tgaagaagaa
    23101 aatagaaaaa gttttgagag aggagaatgc aaactcatta gaattaaaag gaaaaataac
    23161 gtttaaaaaa ttttacgaat ctaaatggct tcctagatat gaattaggtc aaacaattag
    23221 atcaaacagg cctcctagtg atataaccat tagtaataca aaagatatat ttcggttgca
    23281 tattttacct atgtttggtg aatacgcaat gaattatcta aatatcaata ctgagataat
    23341 atcagatgaa ttaaccaaaa aatcgaaaga atatgcaaat ataaaaatca taaaaggata
    23401 tgttcgttcc atgttcgata ttgcagaaat actaaattat atcgaattta atcgaactac
    23461 taaaattatt caaagtatca ctgcacctaa aaaaaatgct ttagaagaaa aaagaataca
    23521 agaaggtaag caagcactat catcaaaaga acttactaat tggatagaag cagtaaatga
    23581 tgattttaat aatcatttac ttacttttca tgattacaca ctttttatga ttaccctgta
    23641 tcttggtgat agaaaaagtg agacttacgc tttgcaatgg aaatatattg attttgaaaa
    23701 acaaacggtc cgtcttaaac atactttaga taaatatcaa agaaaaaagt ttacaaaagg
    23761 tcgaaaagat accgtcattc aagtaccaga agttgtaatg acattgctaa gtgaatggaa
    23821 atcggtacaa gctgatcaat tgttgaaatt aaaaattaag caaacgttag atcagtatct
    23881 ctttacttat actaaaccat ctggagaagt aaattgtcct gttcatgccg attacctaaa
    23941 ctatcgaatc aattcaatta aaagaaggca tcctgactta gttcatttat ctccacacaa
    24001 actccgtcat acttatgcaa ctatagctag acaaggtgga gcagatatga atcaaatttc
    24061 aaatgcttta acacactcag atatttctac aacaaagata tatgtgaaca caccagatat
    24121 agttgataag gctgtttttg aagcttttca gagaggattg aataagtgtg attgatatgt
    24181 gttcttaaat gtgttcttaa aaacaaaaga agcactttcc aaataattga aaagtgccag
    24241 aaacgttgat atataaagga tattaacgtt ttgagaattg tgaagcttta cgtgctttct
    24301 taagacctgg ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt
    24361 taagagctaa acggaagtca ggatcaactt ctagcaatgc acgagcgata ccatgacgga
    24421 tagctcctga ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc
    24481 cttttgtttc agtaacagcg aaaggttgat tgattacttc acgcaagtca gcatgtggaa
    24541 tgtattcttc aacatctttt ttatttacag taattttgcc agttcctggt actagacgta
    24601 cgcgagcaac tgcgttttta cgacggcctg tgccgatata ttgaacttgt gccaatgaaa
    24661 ttccctccta ttagattaag tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg
    24721 gatgttctgc tccaccgtat acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt
    24781 taggaagcat accttttaca gaagtttcga tcaaacgacg agaattttta gcacgcaatt
    24841 cgccagcagt gattgatttc aatcctcctg ggtagttgct gtgacggtaa taaattttgt
    24901 cagtcgcttt tttacctgtt aatttcactt tgtctgcatt gatgacgatg acgaaatcgc
    24961 cagtatccaa atgaggtgtg aaagttggtt tatttttacc acgtagtaca gatgcaacaa
    25021 ctgttgaaag acgtcccaat ggaacatcag ttgcgtctac tacataccat ttacgttcta
    25081 cttcgcctgg tttggccata tatgttgtac gcacgttatt ttcctccaat ttaagttctg
    25141 atgtttgaca tacacttcga gtttccgggg ctctttgtgg ggcaaacaat accatttaac
    25201 atattacccg ctaatctagt caatgtcaac gtttttctgc ttgattgaaa aatttatatg
    25261 aaactttact tcttttcttt cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac
    25321 aaaaagaggc tgtgacaaaa gtcttgtcac aaccccttat tttgaataaa cggtgttctc
    25381 ctgccaactc ctcggtcaca agtcacaata tccataaaac ataagaagct gttttttaaa
    25441 attgtttctt gtgactcgga gctgaacggc aggcttacaa cctcttggta aacggtgttc
    25501 aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgacg
    25561 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt
    25621 caacaacagt ttttacgcgt cgatctctgg cgctttgccc aaatgagctt ggatcatcca
    25681 gatacgtttt tctgtggctg ttttgaattc gataaagata tcttgtgttg gatcgtcgcc
    25741 ttcttctcca gaaacttcga ttcctttttg gtaaagatct gctagataac gatagcctgc
    25801 aacaagtgtt tccaaacgtt ctggaatagt acgatcccag cggccaattt catcagggat
    25861 ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa
    25921 gcgttcggat acttcatcta actgagcgtt caaatcatcc atgaactcat ccatcattgg
    25981 gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa caacaacaga
    26041 catttggctt agatctgcaa ctaattgatt taatacttct ttggtttgtt ggaatttcat
    26101 ctttcttgcc tcctaacatt ttcttacact taaaataata gtagactttc aatgaaaagt
    26161 acaacaaaat catttacctg agaacgattt tcagttcgct tttttatccg taaagccaat
    26221 catttttcag aatcagctaa ttcttctcgc agttgagcaa attgctgata agtctgtgct
    26281 aaatgctttt gtgtcgaaac aaataatgga tagattttct gatatttttt atgatcagtt
    26341 tcttgtactc caacttgctc tttcaattct atcaattcct gtgcttcact gatactttgg
    26401 atctttccga tgcttctcca gcctaaaatc acagctccta atgcggatgc ttctgtgaca
    26461 gtagggaaaa caagcgggta acccagtaca tcagccatca tctgacgaaa aatcggagat
    26521 tgagcaaacc cgcctgtagc acggatctct gtgagctgat tttcctggca aactcctttt
    26581 aaaatccgat aaaggttgag acagatccct tctagaatcg ccttcatcat ttctttttct
    26641 gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca aagcggtgct
    26701 cgctcaccaa gtaaataggg atgaaaaatc agaccattgg cacccgctgg cacaaaagcg
    26761 atagccgaaa gcagtgcatc atatggattt tctccttttt ctttagcaga ttgaacgact
    26821 tcctgtaaaa atgtatgccc tgcccaatca aagatgccag caccattgct agtcgctcct
    26881 cccactaccc aatgtttctg atccagaaca taacaaaacg tctccatttc tggatggaac
    26941 tgaggctgag tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc
    27001 gtatcaccaa aagcacctaa ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca
    27061 aatgatatat catttggcaa acctagttct gctgctgttt ttgatggtag acctgttact
    27121 tgataggtgg aaggaaagac ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc
    27181 acttcttctg cccattggaa ttcatggata tcaaaatatc cagttcctga agctgttccg
    27241 tagtcgctga tcagttcgcc aaacaatcta taaaagatat agtctttgat accgacaaaa
    27301 taagcagttt ttttgaataa ttcaggttgg ctgttttgca gccacttgat tttgttcaat
    27361 ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa attctttgcc aagcacggta
    27421 tatttcagtt tttctgcttc gtccgctgct cggttatctg cccaagtgat cattcgagat
    27481 aacggtgttt tctcttcatc caacaatatc aggccatgca tcgcagagga gaaggagaca
    27541 aattgaatga ttgtgtctga aggcaactgt ttaattactt cttttataac ggtgcagaca
    27601 ccttgaaaga tcaattcctg atcttgttct gccatccctt gtgtttcttg gatcaaaggg
    27661 tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt
    27721 tgtgtggttc cgatatctaa tccgattgaa acgttcattt tttcacctca aattttatgt
    27781 atgtacaaaa atttagttta agcttgcgta tcttggtttt gaaaaggggg agatggcgtg
    27841 ttactttatt ttatcattgg ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg
    27901 attccgttag gtcactctat tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg
    27961 ctttcttggt atgaattgat tccgattctc tcgatcatcg tccaacgatt tcgctgccgc
    28021 tattgcaggt gtcggttgcc cttctactat ttgttggcag aagccttatg tggtggtctt
    28081 tttgcctggt tctttacttt ttcttccgca agaaattttt cgacgttcat atggatgctt
    28141 tcggctttgc tattttcgtt gatggattta ttttacttca tggtccattc tcacacgcta
    28201 ttctgttctt gggcaatttt atggattttt tggttgcagg ccggcgtctt ccaatggcaa
    28261 agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc gctatggtca atcctatctc
    28321 ggagctggcg atactctgtt gatgctttct tggagtggtg gcttgtctct agaagagttg
    28381 cttcagatct tgtttcttgc tagtctgttt ggaatatccg ttttttctgt ttactttatc
    28441 tgtcataaaa aaaagttgga aaagctgcct ttcattcctt ttttgagcag tgcgttgctg
    28501 attgtccttc atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg
    28561 atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag gttcacaacc
    28621 tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat gctggtttta
    28681 caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt cttgacgccc
    28741 caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt gtaatggagc
    28801 ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc gcttcttggt
    28861 tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa
    28921 cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg ataagaatca
    28981 ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca atagatttcc
    29041 accaactgat cataagaaat aacttctgga tcatattcga tctctactgc ttctgtatgc
    29101 cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc gccagtataa
    29161 ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat acaccaaaag
    29221 catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt ctcatcatac
    29281 cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga aactattata
    29341 tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat
    29401 caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc aatacatgca
    29461 tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc aagcatgcat
    29521 ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca ggttttgctt
    29581 ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc gttttttcta
    29641 aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt gtttttttca
    29701 agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt ttttgaatcg
    29761 cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt tctgagaatt
    29821 tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg gatattcctt
    29881 tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct aaaaccagcc
    29941 acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg aatagtgctg
    30001 ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt
    30061 tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc
    30121 tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt cgcttccttt
    30181 accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc tagcaatccc
    30241 aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta cgattacgag
    30301 gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt cgatgccaga
    30361 gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac cagcactact
    30421 gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca tggcttggtc
    30481 agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc
    30541 ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg ctttaatcag
    30601 ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt ctcccagtgc
    30661 ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg ccatgccgat
    30721 gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa caccttgttg
    30781 caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg gctgttgttg
    30841 gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg cagtttgacc
    30901 gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga ctgctgtttg
    30961 ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc catcgcctat
    31021 aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt gaatggcttg
    31081 tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca gaatacgcat
    31141 tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc tccattgaca
    31201 aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt tgtcaaaaca
    31261 aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga
    31321 agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa accaaatata
    31381 actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga tgaatgcgga
    31441 tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca ttccatttct
    31501 tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc gtggattgtt
    31561 ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata ctcaggtaat
    31621 aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt tacttcgcta
    31681 cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt ggtcaaatca
    31741 ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt gttaggcact
    31801 ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg atcgccagct
    31861 tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata tgcattattg
    31921 tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa atgatcatcg
    31981 acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt
    32041 gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc ataagcaagg
    32101 tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt tccggttttg
    32161 atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg
    32221 aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc taatcgatca
    32281 taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa gaaaggcagg
    32341 ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt tagtcccttc
    32401 gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg
    32461 aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg atgctgatgc
    32521 caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact aacgcttttc
    32581 tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt agagagcacg
    32641 atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa ggaagcggtg
    32701 atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc aatcccaata
    32761 tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc aaatactcgc
    32821 agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc gatacccgtt
    32881 ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag ggctgcctga
    32941 tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt tccttgagca
    33001 gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc ccgctcgtat
    33061 gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat ttcccatctg
    33121 atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg gttatataca
    33181 ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct tcctctgata
    33241 ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata ctctaaatca
    33301 aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc gaacgatata
    33361 aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc atctgagtct
    33421 ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg tcattaactg
    33481 gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt tcgcctggat
    33541 acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct tctctgatat
    33601 tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc agtgtgccaa
    33661 aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc cctgaatttt
    33721 ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta ggaaatccag
    33781 taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt
    33841 gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga agcgcattcg
    33901 cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt gttgcttcgt
    33961 cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg gctgtctctg
    34021 ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc actgaagcat
    34081 tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta cagacactca
    34141 cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca tgagaaaatc
    34201 ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca gcggccaatt
    34261 ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct acttgtccag
    34321 cgcctatgat tgctactttc ttcatttatt tctccctcct ttgcttattt acttattgtt
    34381 attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca gacagttttt
    34441 caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca aacgacattc
    34501 atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc gagtaaaatc
    34561 aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat gacccacata
    34621 ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca tatgataaaa
    34681 atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac gcgcatccgc
    34741 taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa taaccgtacc
    34801 tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt tatagtctcc
    34861 ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc cccaagctgc
    34921 tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct cagcaatttg
    34981 acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat cgtagccgtt
    35041 acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg tgtcgatatt
    35101 ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt tgataaatct
    35161 aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt
    35221 tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg gtctacataa
    35281 cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa
    35341 tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt acggttccat
    35401 cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt tgcattattg
    35461 aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa ggcaacataa
    35521 gaaatgatcc cgccatagcc gatccctctt aaagaagctt gtaatgcggc agcactgcca
    35581 ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag acgaaaaccg
    35641 acatctgctt tcataggatc tagcgtgtaa tcggcaccgt atttttctgc taattctcta
    35701 cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt agctaattga
    35761 atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc tgctcgaaca
    35821 tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac tgcatttttc
    35881 cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt tgtaatcagc
    35941 gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc tgtcaccaaa
    36001 ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc gccaaatacc
    36061 acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc tgaaaattct
    36121 tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg ttttggagta
    36181 gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc gtaatctttg
    36241 aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt cctctttctt
    36301 ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc gttcagataa
    36361 cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca ttcgtcccca
    36421 aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga
    36481 ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa agcaataaac
    36541 gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa tgatctggat
    36601 ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata
    36661 actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga taatcagtgc
    36721 tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa caacgatcaa
    36781 aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc gagctttgaa
    36841 gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag ttacggaggc
    36901 tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa tctcagcatt
    36961 gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc
    37021 aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt
    37081 gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct ttttattttt
    37141 catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat gatgaacaat
    37201 agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt ctgatacaaa
    37261 taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa catatagaac
    37321 atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc cggcagtgtc
    37381 acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc ataaagcgtt
    37441 ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata gtatctccag
    37501 atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca cagcggtgct
    37561 tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata actattattg
    37621 aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact taataaaatc
    37681 agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc tacggctaat
    37741 cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa acctaaagaa
    37801 cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat aaaaacggcg
    37861 tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga aagcattgga
    37921 atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat ggctaatttt
    37981 aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc tgccaatagc
    38041 tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt tttttgtgcg
    38101 tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac tgcttttccg
    38161 atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc gactagtcca
    38221 aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc aaaagacttg
    38281 atgacttcat tttctttgta accatccagt tcgactactt gtttattcac tggttgagca
    38341 ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa gaaagctgtg
    38401 aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt caatccagat
    38461 actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat attttctgag
    38521 ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat tggtgctgtt
    38581 ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat cgtgtattgg
    38641 gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat cgttacctcg
    38701 ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa cgcttgctca
    38761 ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc tttatctgtg
    38821 aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa accagcttct
    38881 gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg tgcagcaata
    38941 tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata attatcttct
    39001 ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc tttatccatc
    39061 actttagcaa aatcctgact gacttccatt acttctggta atttaccgga acgagctaac
    39121 gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat tttgatgtct
    39181 ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg ttcctgttcg
    39241 acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct ttgactttca
    39301 ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat aatttttttc
    39361 atttttttcc acctcttatt ttattatttt cagccacata tattcatacg gctggaatat
    39421 caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta ggtaatctcc
    39481 ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta agactttctc
    39541 ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta agtaaatcaa
    39601 actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg tcaattcacc
    39661 atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg ctaacaaaaa
    39721 tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt aatcatttcg
    39781 acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta ccaaaaatga
    39841 atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag aacttaacgc
    39901 atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt ttttcttgta
    39961 gtttactcgg gtaccctctt tcttcagctg ttccactaat tcaaggatcg ttttttccgg
    40021 cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa
    40081 agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact tacttgtatt
    40141 acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga ccatctgtgc
    40201 ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat ttgtctcagt
    40261 gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt ggatgatttt
    40321 atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa atcccacagc
    40381 gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat cgatcatttt
    40441 caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa aagtcgtcca
    40501 tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc tggtccttgg
    40561 tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt
    40621 atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat gattgcagac
    40681 aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc atgtgcccaa
    40741 acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg tatatgggaa
    40801 aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat aaaatttctt
    40861 aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag taatcaagaa
    40921 aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct gataaacact
    40981 gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc
    41041 ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt cccaacgatt
    41101 tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat tttgatatac
    41161 acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta aaaaaatatg
    41221 gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa aaaagaatgt
    41281 ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa
    41341 acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg tctccagagt
    41401 attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc aagcaatcaa
    41461 agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg gttttgacgc
    41521 gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt ttttttcaga
    41581 tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga ttctacaaac
    41641 ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat
    41701 ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg aattggatca
    41761 atacaatatt cctattgtgg taatcggaaa agtcggaaac gactacacga acatctactc
    41821 agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga tcaagaatgg
    41881 acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca atgatcgtct
    41941 caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg aatggattat
    42001 agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa tgaagctacg
    42061 agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta tttacaaagt
    42121 ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat tcagtgatcc
    42181 cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga caaatcagtt
    42241 aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc ctgaagaaaa
    42301 aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac aagaaatgta
    42361 gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg ttcttttttc
    42421 aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt attttccatc
    42481 tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt ccacagggaa
    42541 gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt tttaaacaca
    42601 tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca taaatagaat
    42661 aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata gctagcaaaa
    42721 tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa aatgatctat
    42781 ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct taattggata
    42841 cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa ttattttaga
    42901 caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa aatatctatt
    42961 ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct gaaatactgt
    43021 agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa cgttgcatac
    43081 cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca agaagaggat
    43141 ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg aatgatgcag
    43201 cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag acaggtcgag
    43261 aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta aagtctgcga
    43321 tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca cgaactttac
    43381 gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc ccttgcatac
    43441 ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca tcgcgaactt
    43501 gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg tggatcatat
    43561 cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt gtatctgttt
    43621 ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata tcgatgacat
    43681 cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca aagatttctt
    43741 ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta tggaatgtac
    43801 gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta ccaactgctt
    43861 ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt ttgcagacac
    43921 catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca cctgcatcaa
    43981 cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc aattcatttg
    44041 tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct tctaatgatt
    44101 cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca gtaccgcaat
    44161 cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc aagtaacctg
    44221 aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga gttgagataa
    44281 acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat tccatgattc
    44341 gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag ttggaaatgt
    44401 taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc aaaccttcca
    44461 tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca cgttcataac
    44521 gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca acttgtttat
    44581 gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg atccctactg
    44641 tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca agcatcttag
    44701 aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct aagtttttct
    44761 tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca gtaccagctt
    44821 caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt tcattcagat
    44881 aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt gtgatcatcg
    44941 tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg ttcacaccga
    45001 tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg ttttgatctc
    45061 tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag tagttaccta
    45121 gaaccatatc ttgggaagga gtaacaactg gtttaccatc tttagggttc aagatgtttt
    45181 gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat ggaacgtgga
    45241 cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt ggatgcaaac
    45301 ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt cggtgaagcg
    45361 taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa acatcccata
    45421 cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa gctagttcgc
    45481 gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt tcttttggaa
    45541 gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga cctgagtagt
    45601 cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc aacatatgag
    45661 aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga cgaccgttat
    45721 cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg atgattccag
    45781 gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca cgacggtaaa
    45841 gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt gggcgtaaat
    45901 ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt cctgaagcgc
    45961 ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt ccttgtgcag
    46021 atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta tcaagtaatt
    46081 gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt tcacgttttt
    46141 cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt cctggttcga
    46201 tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc atgtctaata
    46261 caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca ggagctgcta
    46321 attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc actccgcatc
    46381 gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca cattcccagt
    46441 cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt tttaaggtac
    46501 gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg attttttcgg
    46561 gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa aggggttccc
    46621 tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt gcagctcata
    46681 gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca tcgtcttttt
    46741 catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc tgttcggcaa
    46801 attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg tcgcgaagtt
    46861 cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat tgcaattctt
    46921 tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca cccttgacga
    46981 ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc aagatttctt
    47041 gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca ccaaaacgtt
    47101 gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag taaggtccga
    47161 ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg tacatcacac
    47221 cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga acagttttcg
    47281 catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca cttgcaccat
    47341 caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc aagtgtaatt
    47401 ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc atgatatcga
    47461 ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg gatacaaccc
    47521 ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt ttttgaacga
    47581 tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct tcacgagtaa
    47641 agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat gatgtatcgc
    47701 ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct gcagataatt
    47761 ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg acttctgcac
    47821 caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg acattaggga
    47881 tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat tcatattctt
    47941 caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg atgatcgcat
    48001 cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg cctagagcca
    48061 tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg actttctcac
    48121 ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga cggaattttg
    48181 ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca gaagcatcaa
    48241 cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca tgcgctgatt
    48301 tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc actgcttgac
    48361 gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag aaaggaatac
    48421 atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct actttatcaa
    48481 tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga gcaaatgttc
    48541 catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg atatcagctg
    48601 tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga cgataaggtg
    48661 tttcgataaa tccgaattta tttactttcg cataactgga caaactattg atcaacccga
    48721 tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag tagtgaacgt
    48781 cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag gctgaaagac
    48841 gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt gacaactgag
    48901 aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc aattgttgag
    48961 gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt tccatacgtg
    49021 ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata cgacgatttc
    49081 ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag aaatagctca
    49141 ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga tagccgtttc
    49201 cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg acttggacag
    49261 tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa tttaggccat
    49321 tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt cctttttcaa
    49381 cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga ttcaatagac
    49441 gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct aagtcgtaac
    49501 gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct gtttttggtt
    49561 cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt gagtcgcttg
    49621 cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg ccgaagattt
    49681 caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt aaaggaatct
    49741 tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc atttctagcc
    49801 aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt ttgtcgactt
    49861 ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact cgctctgccc
    49921 cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag aatacttctt
    49981 gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt aatggtgcag
    50041 agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct ttcaattcat
    50101 aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa atgtcttcaa
    50161 acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga atttcgatca
    50221 aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg cgatgttttc
    50281 cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat tcaaaaagac
    50341 tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca acagacattt
    50401 tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa ccttacttcg
    50461 tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt cgtttctgcc
    50521 ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct ataaaagtca
    50581 acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt caattttatg
    50641 ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta tactttagaa
    50701 aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc accaacacaa
    50761 ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga tggatacaaa
    50821 cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag caaaaagcca
    50881 cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta tcaagcattc
    50941 tactttttca atttattttc cgacttttca atccatttgg acattttgtc tgaatggatt
    51001 ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc atcggattag
    51061 gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc gcagaggcat
    51121 cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg ttagatgctg
    51181 cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc gataatctaa
    51241 caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg agtgagaaac
    51301 tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca caatctttat
    51361 taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg gaacaagccc
    51421 atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca gatatggtgc
    51481 caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa acaatcggca
    51541 tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca gaaaaagaat
    51601 tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat gatgtccaac
    51661 aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact gataacacgt
    51721 tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca cctgtcgttg
    51781 ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc gactacgaaa
    51841 gcttgggtaa acaaacaggg atcatggcag caaaaatctt agacggcaag aatccaagtg
    51901 aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac atggccaaag
    51961 cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga ggattctcat
    52021 gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat gggccctact
    52081 tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga ctgctgaagg
    52141 aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct atcatccatt
    52201 gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt ccggactgct
    52261 tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga ctggtttata
    52321 ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc agccaacgat
    52381 ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga tcgtcggtct
    52441 gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag aaacaggact
    52501 tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga tcaacacaga
    52561 taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt ctggctcctt
    52621 gcttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga tcgtaattgg
    52681 acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat ttctccgtct
    52741 cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag tcttcgaact
    52801 gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga tctgtctttc
    52861 tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg gtagtgtcaa
    52921 atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg aacgatcaac
    52981 gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt cgtgacgatc
    53041 atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg tacattttct
    53101 gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc tgttgtcgaa
    53161 cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc cgttcgttta
    53221 acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg cttcttccgt
    53281 ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct aaatttgggg
    53341 ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg tcaagctatt
    53401 acgttattga tggccacatt acagcgtcca gaactgatct tattagatga acacactgca
    53461 gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct gatcactgag
    53521 cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg ctatgggagt
    53581 cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga ggaaaaacag
    53641 cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac ggcattaaaa
    53701 gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa atgaacgccc
    53761 accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa ccgttgtttc
    53821 ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa ggagcttgat
    53881 gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga ttttctttcc
    53941 ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa agccatcaaa
    54001 gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg ttacctgccc
    54061 tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat tcctgatttg
    54121 aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgtcaaact ggcagcagct
    54181 caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc tcgtggacgt
    54241 tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct cttgctccat
    54301 ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag tgcctgtgtg
    54361 aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa tgatatctac
    54421 atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat ggaaagcggc
    54481 aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca aaatcatttt
    54541 ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc tagtgtcaca
    54601 aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc tcacccagaa
    54661 aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt gtcgttcaat
    54721 caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg cgaactgatc
    54781 gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct cacatctatc
    54841 cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag tgtccttttt
    54901 tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct ctatcctcta
    54961 gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa tgaatcaatg
    55021 caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc gcttcctttt
    55081 tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg cgacttattt
    55141 tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa cgtctcgcat
    55201 agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga cgcattcctc
    55261 gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa ggttccagcc
    55321 ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc agatcttcta
    55381 tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct tgttcgttat
    55441 ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt ggagaagttc
    55501 ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc agccaagtca
    55561 ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc tcaatagaga
    55621 cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg catagcatat
    55681 ctaaataatc catttatttt cctccttttt atttatccta cttacttttt cctaattcta
    55741 ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca gtcaccatca
    55801 tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga acaagaagca
    55861 acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa acgaacagtt
    55921 taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga aggggaaaaa
    55981 tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc ttttcgtatg
    56041 gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt tataatgatc
    56101 taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt gctaaaaata
    56161 agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt gatgcaaaaa
    56221 ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat tatatacaaa
    56281 aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga atgaataaat
    56341 ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca gtcaatggat
    56401 ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg catgaatata
    56461 ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt gcaacgatca
    56521 ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg acaatcgatt
    56581 taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa gtgttaacga
    56641 tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt aatgtcatca
    56701 acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt tcaaatcatg
    56761 aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag atgactgatt
    56821 tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact atccgcatta
    56881 ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca gctaccaatc
    56941 gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca gacacaagcg
    57001 acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DANKBF010000008        55430 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000008 DANKBF010000000
VERSION     DANKBF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            3..371
                     /locus_tag="PWY89_000533"
     CDS             3..371
                     /locus_tag="PWY89_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL2065593.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="PWY89_000534"
     CDS             551..1492
                     /locus_tag="PWY89_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL2065594.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="PWY89_000535"
     CDS             1561..2292
                     /locus_tag="PWY89_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL2065595.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="PWY89_000536"
     CDS             2436..4616
                     /locus_tag="PWY89_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2065596.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="PWY89_000537"
     CDS             4636..5118
                     /locus_tag="PWY89_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL2065597.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="PWY89_000538"
     CDS             5134..5895
                     /locus_tag="PWY89_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2065598.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /locus_tag="PWY89_000539"
     CDS             6026..7360
                     /locus_tag="PWY89_000539"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2065599.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="PWY89_000540"
     CDS             7667..8152
                     /locus_tag="PWY89_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL2065600.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="PWY89_000541"
     CDS             8145..8828
                     /locus_tag="PWY89_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL2065601.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="PWY89_000542"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="PWY89_000542"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2065602.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="PWY89_000543"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="PWY89_000543"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL2065603.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /locus_tag="PWY89_000544"
     CDS             12444..13073
                     /locus_tag="PWY89_000544"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL2065604.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="PWY89_000545"
     CDS             13125..13457
                     /locus_tag="PWY89_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2065605.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="PWY89_000546"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="PWY89_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL2065606.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="PWY89_000547"
     CDS             14283..15656
                     /locus_tag="PWY89_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2065607.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="PWY89_000548"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="PWY89_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL2065608.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="PWY89_000549"
     CDS             16711..17151
                     /locus_tag="PWY89_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2065609.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="PWY89_000550"
     CDS             17295..17996
                     /locus_tag="PWY89_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL2065610.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="PWY89_000551"
     CDS             17993..19114
                     /locus_tag="PWY89_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2065611.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="PWY89_000552"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="PWY89_000552"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2065612.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="PWY89_000553"
     CDS             20493..21401
                     /locus_tag="PWY89_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL2065613.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="PWY89_000554"
     CDS             21436..21708
                     /locus_tag="PWY89_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2065614.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="PWY89_000555"
     CDS             21719..22765
                     /locus_tag="PWY89_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL2065615.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="PWY89_000556"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="PWY89_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2065616.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="PWY89_000557"
     CDS             complement(25776..26462)
                     /locus_tag="PWY89_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065617.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="PWY89_000558"
     CDS             complement(26440..26934)
                     /locus_tag="PWY89_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2065618.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="PWY89_000559"
     CDS             complement(26954..28174)
                     /locus_tag="PWY89_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2065619.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="PWY89_000560"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="PWY89_000560"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2065620.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..31263
                     /locus_tag="PWY89_000561"
     CDS             30352..31263
                     /locus_tag="PWY89_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065621.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(32203..33162)
                     /locus_tag="PWY89_000562"
     CDS             complement(32203..33162)
                     /locus_tag="PWY89_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2065622.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            33325..33576
                     /locus_tag="PWY89_000563"
     CDS             33325..33576
                     /locus_tag="PWY89_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065623.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            33659..33931
                     /locus_tag="PWY89_000564"
     CDS             33659..33931
                     /locus_tag="PWY89_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065624.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            34021..34260
                     /locus_tag="PWY89_000565"
     CDS             34021..34260
                     /locus_tag="PWY89_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065625.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            34407..38690
                     /locus_tag="PWY89_000566"
     CDS             34407..38690
                     /locus_tag="PWY89_000566"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDL2065626.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            38789..39154
                     /locus_tag="PWY89_000567"
     CDS             38789..39154
                     /locus_tag="PWY89_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065627.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            39156..39608
                     /locus_tag="PWY89_000568"
     CDS             39156..39608
                     /locus_tag="PWY89_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065628.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            40222..41532
                     /locus_tag="PWY89_000569"
     CDS             40222..41532
                     /locus_tag="PWY89_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2065629.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            41555..41866
                     /locus_tag="PWY89_000570"
     CDS             41555..41866
                     /locus_tag="PWY89_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065630.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            42227..43483
                     /locus_tag="PWY89_000571"
     CDS             42227..43483
                     /locus_tag="PWY89_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2065631.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            43480..44025
                     /locus_tag="PWY89_000572"
     CDS             43480..44025
                     /locus_tag="PWY89_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065632.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            44026..44526
                     /locus_tag="PWY89_000573"
     CDS             44026..44526
                     /locus_tag="PWY89_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065633.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            44607..45163
                     /locus_tag="PWY89_000574"
                     /pseudo
     CDS             44607..45163
                     /locus_tag="PWY89_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            45183..45527
                     /locus_tag="PWY89_000575"
     CDS             45183..45527
                     /locus_tag="PWY89_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065634.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            45546..46457
                     /locus_tag="PWY89_000576"
     CDS             45546..46457
                     /locus_tag="PWY89_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2065635.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            46536..47528
                     /locus_tag="PWY89_000577"
     CDS             46536..47528
                     /locus_tag="PWY89_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2065636.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            47525..47743
                     /locus_tag="PWY89_000578"
     CDS             47525..47743
                     /locus_tag="PWY89_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065637.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            47756..48166
                     /locus_tag="PWY89_000579"
     CDS             47756..48166
                     /locus_tag="PWY89_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065638.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            48218..50746
                     /locus_tag="PWY89_000580"
     CDS             48218..50746
                     /locus_tag="PWY89_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2065639.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            50762..52972
                     /locus_tag="PWY89_000581"
     CDS             50762..52972
                     /locus_tag="PWY89_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065640.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            52980..54071
                     /locus_tag="PWY89_000582"
     CDS             52980..54071
                     /locus_tag="PWY89_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2065641.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            54092..54634
                     /locus_tag="PWY89_000583"
     CDS             54092..54634
                     /locus_tag="PWY89_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065642.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            54829..55086
                     /locus_tag="PWY89_000584"
     CDS             54829..55086
                     /locus_tag="PWY89_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065643.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            55100..>55430
                     /locus_tag="PWY89_000585"
     CDS             55100..>55430
                     /locus_tag="PWY89_000585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065644.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
BASE COUNT        18755 a         9103 c        11900 g        15672 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31201 cgaattatcc aatatgctta tataggaaat ctttctttag aatttttgat ttacgggata
    31261 taaaaatagt ttacgtcgat acttattaag ttatcgacgt tttttattat agaaaaaatt
    31321 tataaaagga ggtttcttga tatgataaga aacaaaaagg gtttgttgaa agttgcttta
    31381 ctggcagctt ttttctaagt aactttttga caagctcaat ggcaacacaa gcggaagaag
    31441 taatacaaga actaactgtt atgaataaca gctagataaa atgggctatg tatgttatcc
    31501 aacaatttag taatcactag aagcctagcg aaagttcgct tgataagtta tagatgagta
    31561 ttgatttgta gtgtttccaa atcaaaaggg tacagagcca catgaaatat gttgtcctct
    31621 gcagcgtcag gatgggaatc cataccatgg agattcggag atctgtgagg gaatacttac
    31681 gtggttaata tgtcagttgt gttggacgta gtaatggtca tagtgggtag attgactgaa
    31741 ttcttatatg agaaaatgaa accaacaaga agatagggtt atttatacag aattggggat
    31801 cttttctgga aaagtaatcg gataatgcga gattcgtacg gtctttttta aatttctcta
    31861 aaaaaaacgg catcgaatgg gttgggtgat tgataagcaa tgaatcatag actgatatag
    31921 aataaaaaat gattctattt atctttttac atctatcgtt ttttcaagta gccgcaaatt
    31981 atatcagcat tcaacaattt ttgtatacaa gttctagtaa aaggatctga agaaaaagtt
    32041 ggttgttgtg aagataaaaa aagaatcagg ttttactgag tttttcttca aaatttgtgt
    32101 tattaggtta ggacaaagat aagaagaagt atgaagattt aaaaaaggaa tgaggaaaag
    32161 atagtaattt ccgattattg gagttttttt atttttcgtt tgtcaaatta agttagacaa
    32221 atcatctttt gtgacgtaac tcaaaaatac ttccattggt gtccgatagt gtaatgattt
    32281 tctaggaata tgatttcgtt tcgatgccac cgattggata aaggtttcag atacaccatt
    32341 aaaatccata gctttataca aaccatcttt acgtaataag ccatttgaat gttcattcaa
    32401 tcctcgttga gaaggtgttc ctggatccgc aaaataaatt gaaatatcgt tggtattact
    32461 aatctttttc caatttgaga attcttttcc acaatcaaat gtgatggatt taaataagtt
    32521 ttttgggaca caacgaaacc attgattcag cctattttca atgtctttag cctgtctgcc
    32581 ttcgggtttc aaggtaatga tgactttaga tagccgttca actaatgtga tgaccgcgct
    32641 tttatgtttt ccacccacga tagtgtcacc ttccaggtga ccaaattcat gatcaaactg
    32701 ggtatagtct gtctttctgt ctcggattga ccggcgaaac ccttgttttc ctcttttttc
    32761 tttatgaccg tttggttttc tttttccctt cattggtaaa tcgtgagtgt caaaggtttt
    32821 atctttaaac attcgataca gtgtgcgtac cgaacaagca ataggaaact ctgctcgacc
    32881 aataaggaca tcaggagccc acccttgaac aacctttttt tgaatgtatt cctgttgctc
    32941 atcaggtaaa acaatcggat gtcttccgca gttacgtttg tttttcttat attgtttata
    33001 gtagctcatt gcgcttccac cagatttaag aaaacgatag accttataaa tagtttgtcg
    33061 agaacgttgt aacagattgg ctacttttga aacaggttgt gattgattga aataagcttc
    33121 tatcattacg agttcagttg gtgtaagatg gttataggtc atttgtactc actccttata
    33181 attctttcgt cggaactatt ttgagtgtag cacaaatgac tttttttagt tgtctagctt
    33241 aattttacaa tcggcgattt ataaagaaga agcgaataag ttgtgattcc tagatttcgc
    33301 gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag tgtttaatac acgaggacaa
    33361 agtttcttag gtattgaagg cacacgtgta acggttgatg ggtggtgtga ttgtttcgag
    33421 gttggaaaac atgttgaact tatggcattt actgagctaa aagataagaa caacgtggaa
    33481 tttttcgaga gtgatattgg ttgggacgat catgaagaag tttatggaaa atttattttg
    33541 cggaagggaa attcttatac gaatggggaa gcataagtag cgatttgtta gagatcattg
    33601 ataggattga aattgtcggg aatatttatg agaatctaga gttatttgag gtgatagaat
    33661 gagtgatggt tttgaacaag tatcaataat taaaaatcaa gttcgggaga ttcttagaaa
    33721 aaaaggctat ttggtagata gttattttga aggagattac gaaacatggg taggtgtcta
    33781 tgctcgtcca gagaataaac ctacctattt agatcctacg acgtcagaag atgcctattt
    33841 acaaaatagg tatcgagtag atggttttaa gcaagacttt gcggagtggt ttgaatggga
    33901 aattgaaaac ggagaagtaa aagaagaata agagtacttg ttgtatcaaa ataagccagt
    33961 agaacaacca aaccagtagc tagtaatgac aataatactg aggaatcgga tcattatggt
    34021 atgattgata tttctcttcc agaagatctt aagccagatc ctgtaacaat tggcaatagt
    34081 gtcatgattt ttgattcatt agatgaagct atgtcatatg gttggaaaga aattaaggat
    34141 gaaggtagta attggtatgg catgagattt ggtgatggtg aagttgccaa aaaagctggt
    34201 attccaattg ggaaatggac agttgatttc cacgaagcag gttctttttt tgttctgtaa
    34261 ttatagtggg agggtaaaat cattgatgaa atgtgttcag ataagagcgc cagataaaaa
    34321 tagaagagac agttcacgtc gttatcactt ttggtaacgg cgttttgttt tattatataa
    34381 aattctcagg aaaaggagga attcttgtgt atgcaaaaac gaaaatgaat tggttgaaag
    34441 cactcgcatt ggtggctttt tctcaattct gtttcttggt gggaactgct caagcacatg
    34501 cagaagaaac gacggctata caacctacga tggaagccat tgcccaagtc gcttctgaag
    34561 tgacagaagt ccaaccagca gaagccgggg tcccagctgg tgaagccact gttgaacagg
    34621 cccctgtgac agaagcgcca acggtcaatt ccaaaacgac tctcgatcag acgattgggg
    34681 aaacacaaca cccagggaat agtcctggta cttcacaacc agttgaagaa actgcctcaa
    34741 ttacaacccc tgcttcagaa acgtccactc tagaaggaga aaaagctgcc gaagcaacgc
    34801 cttctgtgca gccagagtct tctgagaaac aagcggaaga aaagaaacga atagaagaaa
    34861 cagtggaaca accagcggct actgctgaca aagaaggaaa aaatgaggaa aacgttgaaa
    34921 aaacaatctc ttcaaccgca gctagtcaaa atcaaccaac gaaaccaaac aaagaatcta
    34981 cggctagtat tgaaaatcaa aagaaaaaag agcagaacat tccaaaaaca ggtacaccta
    35041 cactgttagt aacagtacca accaatcgtt cggttcgtgt tcgccgtgca accaatgcag
    35101 ctgttttagg tggtcggaaa attgattggg gcgaaaatat tggtgttact tctagtccga
    35161 acaatctaaa cttcacttta aattatatgg ttcgttttga gattaatgga caagtggcgt
    35221 tttgtattga gcctggtgtc tcagccgcag aaggtcaagt ttatacagct actgctttga
    35281 aaaactattt gaaaaattca aatgtacgga aaaaaacaag cttgatctct tatttaggat
    35341 atgtcacaaa taaagacaaa tccgatgaac aatacattgc ggcacaattt atgatttggg
    35401 aagctttggg cacaaaaatc ccaatcaaaa atatccgtgt tgattatgat cgtcgaaaag
    35461 cagaaattca acgtttgatt aataactttg gtgaaaaagc aacttttaat aatcaaacac
    35521 atacggtaaa aattggcgaa acgttgaaag taaagaataa aaatgatttt ttgaaatatg
    35581 tgaaagacat tcaaaaacca aaaggtgtca atgtatctat tcacggcaat gaagtatgga
    35641 ttacagtcac aaaagaggca ccggataaag caaaaattca attgaataaa attaaagttc
    35701 aaggtgcgcc attagcctat cgtcgtccag gagctcaaac aattggtgtg ttacatccaa
    35761 cagaacctaa tgcgtctgtt attaatttac aaattctaaa acaaggaaac attcgtgtgt
    35821 ttaaagaaga tgcggaaact ggtgtgaaag cacaaggtgc agcttcttta gaaaatgcgg
    35881 tctatggttt gtatcaagca gatgggaaaa agattaaaga aattaccttg aaaaaaatta
    35941 acggcaaagt acaggcagaa attaaagatc ttgatcctgg caaatatgtt ttaaaagaaa
    36001 tcaaagcgcc aaaaggttat gtgctttctg atgaagcaat tgaaattgtt gttgaacctg
    36061 gaaaaactgt caatgcgacc gcaaaagatc aagtgatcaa aggaaccatt gatttagtga
    36121 aagttgctaa taaagattta gtcgattcaa cgaatccaga taacaaacca aaattagctg
    36181 gcattgaaat cactttgacg tcaaaaacaa ctggcaaagt ggtgaaaacc gttgtgacag
    36241 acaatgatgg ttatgctact tttggtaaaa ataccgttgt ctttgatacg tacattcttt
    36301 ctgaaaccaa aggaaaagaa gggtataaac tatttgaacc acttgaagtc acaatttctg
    36361 aaaatgggca aactttccat tatgttttag aagataaagt aattgaacaa cgtcttaaag
    36421 tggtgaaagt ggatcaagaa acaggaaaag tgattccact tgctaataca gaattcaaga
    36481 tttttgatac tttagccaat cgttatgtca caatggagat tccaaatgat acagaaacaa
    36541 cggatatctt taaaaccaat gaaaaaggct ttttcctaac taacggtaca cttacttatg
    36601 gaaaagatcg ctatcgtttg gaagaaatca aagcccccgt aaattacgtt ttgaatcaaa
    36661 acccattagt gttctcgatt accaatgata cagaaccgat caaagtgatc cattttgcga
    36721 accgtcaagc acggaaaagt gtaaaattat tcaagtatga agaatggaac aagaagaaaa
    36781 cgccgctcgc aggtgtgaac tttaccttgt ataacggtaa aaatcaagca attggtgaat
    36841 ataccactga tcaaaacggg gaaattttag tgaaaggtct agttgccgat gactattact
    36901 tcttggaaaa tgccccatta gaaagtttcc aaccaaatac agataaacaa gcattcacgg
    36961 ttgcattcaa acaaaatgcg ccagatgatg gggaaacgtt agtggtttct gtacgtaatt
    37021 acttgattcc accaaaacta gaaacacaag cgtcaaataa agccgatggt gaaaaagtgg
    37081 ttgatccatt agaaacgatc gtcattaaag acagtgtttc ttataccaac ttgtttgtcg
    37141 gaaaagaata caccatttca ggagtattga tgaacaaggc aacgaatgcg ccaattttac
    37201 aagatggcaa agaaatcaca agtaccttgg cctttattgc agacaaagtc aatggcacaa
    37261 aagaccttga atttgtggtt aatgctagtg ttctgaaagg tcaaacgatc gttgtttttg
    37321 aaaaactttt ccgtgatggc attgaagttg cagcacatgc ggatatcaac gatgaagccc
    37381 aaagtgtgaa agtgaataat ccaaccgttc ataccacggc tgtctttgaa gacggattga
    37441 cagtcgcaga cccaacagga ttgtttacct taaacgatac cgttgaaatt accgagattc
    37501 ttccaggaaa agcatacttg gtcaaaggga tcttaatgga taaagcaacc ggtgaaccct
    37561 tgcttgtgca tggaaatacg gtagaaacga gtgttggctt tattgccaaa gcagaaaatg
    37621 aaacggtgat cgtaccattt acctttgatt tgacaggtat aaatggtcgt gaaatcgtcg
    37681 tgtttgaaag tctttttaga aatgatgaag aactagcaaa ccataaagac atcaacgaca
    37741 aagggcaaac ggtacgagta actaacccaa gtgtacgtac aacggctaca cataaaccca
    37801 ctggcttgaa agaggttaat ccattaacga aagtcaccat tacagacgct gtgaaatacc
    37861 acgatttggt cgttggaaaa acatacacag tcaacggtgt tctaatggac aaagtaacgg
    37921 aaaaaccagt gctagtagat ggaaaagaag tcacaagtac cttaacgttt gtggcagaaa
    37981 caacggatgg tacagtcaat ctagacttta ttctagacgc acgtgcgttg cgaggcaaag
    38041 aagtcgtggt ctttgaagat ctgtttagag aaggcgtatt attggcttct cacgcagata
    38101 ttaccgacaa tgaccaaacc atcaaaatca gtaatccaac cattacgacg aaagcgacga
    38161 ataaaaatgg cggaaaagag atcttggcct tgccaaatca aactgtgctt gaatgggtca
    38221 aagtagacgg tcttgcgatt ggtcaaatct atcgcttaat tgttcaaggc tacttaacac
    38281 cagacggcac accatttgaa ggcactcatg cggaaaaagt ctttgcggcc gataaagaaa
    38341 tgatggaatt cttatttgaa ttcttagtgg acggtcgcaa tcttgcagga aaaggcttga
    38401 gttttgcgga atggctccaa gcgaaaacgg aagatgagaa agaaacgttt gaagaagtgg
    38461 cgaaacacaa tgtagatcta acaaacaaag atcaaacggt tcaaattgtg gaagcgccaa
    38521 aaccaccgca accagtgaaa ccagaacaac gggttaaacc agctactgtt gtaccaatgt
    38581 tgccaaaaac aggtgaacaa acaagtagta tacttatggt tcttggatta gtagtattag
    38641 tcattgcttt aggtataaca ctttataaag tacgtaaagc caaagattag tcatgaggaa
    38701 caagcggtta ggatttttaa ccgcttgttt gattgtaatg tagaaatatt tagaatttat
    38761 tttggttttt aagaggagga aaaactttat ggcagaaaaa gcattgcgta tgaagaaaga
    38821 gtttttaggt caagtatttt tattgggttc acgggaacct aatccaccaa aggaaattaa
    38881 tggtgtgtct gggcgatatg ttcaattaca agcagagtgt tcgcgagaag aacagcctgc
    38941 tttttttgtt tatctcacag ctgaagagga taaaagatta cgattaccag aaaaagaggt
    39001 gcgtatttca tttgaagaag cttattttgt ttttcgtaaa gaacgagtga atagtcgtga
    39061 aacaaatgat atgggacaaa cagagcgtcg aacaactggc tatcaagatg ttggtgtctt
    39121 tttaactggt gtaaaaattg gggaggtttt gtaagatggg attaaaattt ccaaataatt
    39181 atgacattac agaagcatta gatgaaactt gtttaggagc cgtacgcttt ggcgcattag
    39241 aagagtctga tcgtgtattt gataatgaaa caggcgatta tacgggagaa attcgatcac
    39301 agcgagcaca attagttttt ggaaatccag atgaaccaaa gattgcacga cgagttaaat
    39361 ttccagcaga tgtagatttg actggtttta aacgacgtga tcctgtgaaa ttaattgatc
    39421 cagaatatca tgtggtatat attccagcac aagatggcaa tcgtgaatat tcagatttag
    39481 tgatttatgc gaaaggatta gagaaagtag aagctccaac aaataatcct aatatgaaac
    39541 aatcacaaga aaaaggttta gatcaaaaac aaccaagtgt tgattctaag gaaaaagaga
    39601 aaaaataagg tttttttgaa agaataggaa ggagtcttcc tattcttttt atctaagata
    39661 gaaggaaggg gagattttga attcagataa aaccaatcaa atactttgag agattgtgct
    39721 tgttatcaaa atatccaaca attgattgag tattttggag acaatccaac gatttttgta
    39781 caaacaaatg catatatttg tgcggttatt gggcagtgta tgtttttacg tgatcttgtt
    39841 cgtttttcat taccgtacca gtggaaacaa gatcatcagg aagtgatttg gattcgtata
    39901 gtacgaatat gtaatatgtg tgcacaccct tataaaaatc gattaaatct ttttcgatta
    39961 tgttttttta ctaaatgtga tattcctgta ttagttgctt tttgtcaaaa gtttttagca
    40021 caggaaaagt aaaaacagaa aggaggaatg tgacaattga aagctcattt tttcaagtat
    40081 cgtggtcggc gaattcgcta tttcaatcgt tatttatggt tttgctatta tggtttcttt
    40141 ttgtttccat ttttgattct gttaggttgg tttagttgga aagtatttta tccacgttta
    40201 agtacgtttt cgaattggca aatgtatctt attcctggaa taagtattgt gttgtatttg
    40261 ttgctggtta gtgggttaat tttagggttg atgcattggt cgcgactaaa agaaggatat
    40321 tttgcctctg tttattggcg tcagttactt gttcgaatgt taattgacaa tcggtttgtt
    40381 tatacaaaga aacaagttag tgagaaacaa acaagggaaa aaatgagatt accaaaagtt
    40441 tatttttatc aaaagaaaca agaattagtg gtttcttttc ctttagatgg tgggcgtttt
    40501 cacgaacgat ttttaaaaat gggtcatttg ttatctgaag tatttttgcg tgatttaatt
    40561 cgtgaagaac gagaaaaagc ccgaatcaat tatgtattcc tttcagattc tggtcggatt
    40621 cctattgatc aatgtatagc agaaaatgga cgtattcatt taatgaaaac tttggattgg
    40681 gtatatgatc aatcaccaaa tatgttgatt gctggtgcaa tcggtggtgg gaaaacctat
    40741 ttattgtatt ctttaattca agcttgttta agtgttggga cagtagatat ttgcgatgga
    40801 aaagcggcag atttagcagc attaggtgac gtcgatgtat tcaaaggaca tgtgttttac
    40861 aaaataaatg aagaaatgat tatttgttta cgaaatgcat taaaagaaat gaatcaacgg
    40921 tatgcctata tgaaaaccat gaaagatcct aaatatgaac cagggaaaaa ttatgcttat
    40981 tatgggttac caccgcattt tatctttttt gatgaatggg cagcttttta tggttcgttg
    41041 gatatggaag cgagaaatag agtcgataaa ttgattcagc cattagtatt aaagggtcgt
    41101 caagcaggga tgtacattat tttagcaatg caacgaccag atgcagaata ttttccaagt
    41161 ggtgttcggg ataatttaac attccgtatt tctgttggtc gattgtctcc cattggttat
    41221 ttaatgactt ttggagaaga ttttaaaaat aagccatttt acaacaaaga tcaacctggt
    41281 cgtggatatg cttttccagg atttgatgta cctgtagagt tttttgcgcc atttgtttac
    41341 aaagattatc actttatcaa agaatttgag aaatttactg aaatgattgt ctatgatttt
    41401 acagtaaaag aggatagtca agatcaaacg atgggagatg ataatcagtt gttacaagaa
    41461 caactagagc gccaaatgta tttagcaaaa gatccaaaag gaacaagttt ttctgatgcg
    41521 catattttgt aatcaactaa ggaggtgaag ttaaatggca gagagttgtt attatagagt
    41581 agctacagca atacgaatga ttaatccttc tctttcttcc agaacgtttt atgattggtt
    41641 gaatcgtatt gaacaagtaa ctgattaccg ctttttgcgc aaagaacgtg tttttacagg
    41701 aaaagttatt aatcaagtat tgctaacgaa aaaagatata gaacgattga ctcgattgta
    41761 tcattatcga gtagatttag aagaggattt aactttgtca atttatcgcg ttttttctcc
    41821 tgaaaaatat agtgaaataa caaaattaga tcatttaatt ttgtagttat tatctatcgg
    41881 gagggctcta gacgtagtct ggtagccgca ctctgcctgg cggtgcgcat taaggctgcg
    41941 aagcatgggc ctataatgcg cactcgctag cagcgtgtgc agctcacaaa gaggtcgtgc
    42001 gcttgcgcac ggcctctttg tgtaaagccc cccgatatat aatatggggg gcagaaataa
    42061 cataacacat agaaaaaagt tttataaacc cttttgtatc aagtgattat ctaaggtcag
    42121 cagttgtact acttttccca aaaaacgagg gggactagtt gatatatcgg tgtttctaaa
    42181 gggttaaaaa aaatcgattt tgttatttta taaaaggagc gagaaaatga caccgattga
    42241 gctaaaaagc tacagaaaga agttaagtgc aattcataat ataagtggag ataaaaagcc
    42301 gctatcacaa gcaagatttg cgcaattgat gggctgctca aaagattaca ttagaaagat
    42361 ggaacaaggg aaaagaaaaa tcccagagga ttttgaacaa cgaatcaagg aacgctttcg
    42421 attgtatatg aaaagcggaa tagatcttcg agcaatgatt gattatttgc gtctttcctt
    42481 ttttgggaca gactacaaaa cagtgattca acgtgtatta cgaatggatc tgacagaatt
    42541 tgaaacacgt ccaacgggct tgtatctttt tgactgtctt tctgttcgtg ggaatatctg
    42601 ggtgttctgg cattcaaaac gaactgtaac agaaaatgta cttgtgcaat tttcaggaca
    42661 aggttgccgg gaatatgagg aagtcatgcg tgaacaacaa acagattggc gaacatggct
    42721 atattccatt tggaaaaatc aaggaaagtt ggaagctggt tatacacgtg tgcaatgtag
    42781 ccgattcgat attgcgttag atgaactttg gggattggaa acggaagaac atttcgatct
    42841 ctttgaatta ctagaaaaac aaaaagcagg tttattagaa atggacttta agagattcca
    42901 gaatattggc ggttgttttt tagatgatgg ctattttcga agtgaaggat tatctttgta
    42961 ttttggatcg cgaaagagtc ctctcttttt taatttttat gagaaacgtt ttgaaattgc
    43021 gaaccgagaa aaaatttcag aattagaagc attaacgaaa tatggtattt ataatcgata
    43081 tgagttacgg ttagccaatg agaaagcaac acaagcagta gaagcattta ttttaggtga
    43141 ttcaccgaaa cgtttaggag agattggcgt tggtttaatt aatgcaaaac ttattgtttg
    43201 ggatagactg gaaagtgatg cacggattgt tgataaacga tgggcaaaat tatttcgttc
    43261 gatggaagaa ttggatttta cagtaaagcc agaaaaattc tcattagatc gttcagaacg
    43321 ctggtttttg aaacaagtag ctccgacatt aaagttgtta caagaagcag atcgtatttt
    43381 aggaagagac tttgtacaag aagggattga taatgccgaa ctaaatgacg agcaacaaaa
    43441 ctatctagaa tggatgggaa cgtcaggagg gaaaattgca tgaatgcata tacgacaaca
    43501 gaatggctgc gcctgttgga tttaaaggct gcaatcgaag cattaaatga aaagatggtt
    43561 gatttgtctt attttcgttt tcgagttcct tatattgaac aagcggtaaa agctggacgt
    43621 tatcaggaaa aagagaactg gcaagaaatt gcacgcttat tagaagttcg taagggatac
    43681 gaacaagaac tcgaagagtt ggaatttagc cgaaggaaag gaaggctaga attcattcgc
    43741 ttttatcgat tttcattgcc aatacctgcg attttagcgg ttaaaaaagg ctgcgataaa
    43801 atgaagatat atgaaaactg tgtggcagca cttagcagcg aaaaacctct tatggaagaa
    43861 atttcattgg tgactgtcac ttgggaaatg agtcgccagc caacagaaga acagacatat
    43921 ctttctttag aagagattga gatagaatta gaggaaattg gtcgctatgc cacttgttct
    43981 acctattgtg gatcggtcat tagtattgct ggtgtgattg tctaaatgaa aaaaattatg
    44041 atttatgttg gtgcgtattg gtcacgtgat ccaacagtat tagaaaatcc agaggcggta
    44101 cactattgct tgcgccaatt attctatctg tataaagaac gattagaatc gttgattcgt
    44161 cagttaccat atactgacag acgtttagat gaacttcttc taaggtatcc agcaatgtat
    44221 aaacgacgaa aaaatagatt gttaccagaa gagtatccca ttgaaaaaca tgaacttgaa
    44281 gggcgatttg tggcttattt ctatgatgat gttcgtatgc gcttagtaga acaacgaatg
    44341 gaaatagaaa atgatcgcta ctattttatc aattattgta agaagcgtaa gtatcaagta
    44401 actgatgatt tttatgattg tattttacaa gatggggaag tgattctatc caaaattgct
    44461 ccaagttttc gtgaacttgt gccgattgat tttttgaaaa aatgtcattt gcggattctt
    44521 ccgtaaaaaa gagctataaa aggaaagaaa agtagaagcc aaatcggttt ctgctttttt
    44581 atttttttta ggaaggagaa gatggtatgt cactggaatt tttaggagag ccaattgaaa
    44641 aggaagagtt cgtttcgcat tatatggatt tgtttattca ggtgataaaa aactttgtgc
    44701 gattgatgaa ttttatccac gcgaacaaga ctatctagcc gctgaacgaa taaaaatcag
    44761 tttattatgg gaaaaattca cagatgaaaa taaccgaccg ccagattatc gctatttatc
    44821 agattgttta acgaataatg tgctttctaa agaataccta tttcacaaac gtgagaaaaa
    44881 tcaaatgtcg gttgaacatt tttgtgattg ttatattgca ttattgcgta gtcaacagtt
    44941 attaacagta gaacgatatg gtgcagagtt tggttatgtc atgcaagctg agcgtcatag
    45001 agctacaaga tgctatgaaa aagaagatca agtgattgat ggctatgaag aattatgtat
    45061 tcgaaacaat cgctatttgc aacaacgttt atatgaacaa ttgatgatgg atttttcaaa
    45121 tctctataga gggtatgaat gttcgatgat tcaagaaagg tagaagaaaa gggggaattt
    45181 ttatgtggcg attattattt ttggtggtat gtggtatggc attgttctat gtgtatcgtg
    45241 tctatctcta tccacgaatg gaacgatgga caagttggta tagagatcct ttaaaacaac
    45301 gaaaaggatt gaagcggtat cgttatgaat tacttgttga attttcggat gggcgacaag
    45361 cacagtatta tttttttgca aactatccaa aatacaacac cagtgaacta ttgattcaat
    45421 tattcaaaaa taatccatat gttgggttag aagaaaacgg acgggagttc ttttatcgtt
    45481 cagataatat tagtgcggcg tctatgaaca aaacacgaat tgcataaaaa attggaggaa
    45541 taatcatgaa aaaaacaaca ttagcagtcg taacaacact aggaatttca ttattttcgt
    45601 ttcaattagg gagttcattg gtttatgcag atgaaactgt agaaacacct aagtcagaaa
    45661 ttgtagaaac attgccaggg gaaggattac cagatgttga gacagagcta aatggaggaa
    45721 caacggaaaa accagcagaa cctgaacaat tgcagtcaga gaaacctgaa aaagagccag
    45781 tgacaccaga accagagttt cctgaatcaa ctttaccgac tgattcgatc gaagaagaag
    45841 gtgtgcatag ttcaacaaca acggattcaa gtatagtcac aactccggaa caatcaggaa
    45901 caaccagttc ttctgaaaag aatactgatt cgtcaagtac ggaacagcca acgatgtcgt
    45961 ctaccagtga agaacaaaaa gaagaaccga aacaacctgt tgaaaaatca gaagcaattc
    46021 caaaagaaaa taaacctatt gttccagcaa aagaagtcac agtatcggta accccaagcg
    46081 gtgaaattac gacgaatact tctcaaggaa tgagtgtacc gattgtcact agtaatgtag
    46141 aggaattgac ccatattcca acaccaacca ccccattaaa agtagcaggt gatcaaacaa
    46201 ttgttggtgt aaaagatggt attccattaa tccaagatag tgaagggaat ttggtaaagg
    46261 atttgtcgat tcctgtaaag aaacttccaa gtggcaatat tgaaatcaaa acagcagatg
    46321 gaaaaacaaa agtcttacca aagactggtg aagaaataca tgttgcactt tctgttctag
    46381 gaggagtgtt gacaagtatt gctggatttg ttggatataa aagaaagaga aaaggaatag
    46441 aaaatgaggg taattgaatg gactattttt cagaaaaaca agcttatcgt tgtcaatcgt
    46501 acgtactagt aaaaacatac tagaaggagt agcttatgaa aaaacgacaa ctacggcgga
    46561 aagaaaaagt ttatacagag aaagtaccga agcaaaagaa aatccattca aaaaaagcag
    46621 tgaaatggat ttggtgtttg gttttatttc tttgtttttc agggggcttg gcattttttc
    46681 aatccatgaa aacgaaggtt caagtgtcgt ttttgaaaga tgaaatccaa caattacaaa
    46741 agaaagaagt acaaagtcgg tcagaattaa cgtacacacc tgaaattgaa tcctttatga
    46801 atcgttttgt tgctttgtat atgaatacat tcgataatca tgaacaagaa caacaacgtc
    46861 aagaaacatt aaaaaaagag ttttatgcac aaaactttat tcaagaacca ggaagtgttt
    46921 caggtgttcg aaaattaact gctgcttcgt ttattggatt aaaagaaatt gaaaccattc
    46981 caactgctag ttataaagta acttatgata ttcgctcgat gaatccagag agtaagaaag
    47041 aaacaagtgt gacaatcaca caagtactca atcttccctt ttctatttca aacaagggct
    47101 gtcgtattat tgcttatcct tattttacgg cactaccaaa gaatacagaa aaacaacgaa
    47161 tgattactta tgatgacaca ttatacgaaa cggttgatgc gtcggaagcg aaagatgttg
    47221 aaggatttgt ggaagatttc ttagataagt atgcgaaaaa caatgcaaaa gatatgtcat
    47281 acatgatgaa agaacctgaa ggactcaatc aaacagctaa agttgatcaa gtagagatga
    47341 aagtattgcg ttataaagaa acgaaaaaac cttttattgt aaaagcaacg gtcgtctttc
    47401 gagatggtga tacgtcactt ttacaaaaag aacaaatgac gattgtcatt acgaaaaaaa
    47461 caaatcagta ttatgttgaa aaattaacac ataattggtt ggataggaaa ggagaagaac
    47521 ggaaatgaca ctgaagggaa ttgtagattt tatattaacg caaggtggct attttttaat
    47581 aatggtggtt gtttatctgg gattgcgaaa ttttatgaaa tcaaaagtag gaaatgcatt
    47641 aatatctatc gcagcaggtg ccgcagttta tttcttctta aatgatccaa cacgtgtatt
    47701 tgatgcgttt ggtaaaatca ttgaaaaatt atttatggat taggaggata gtgaagtgga
    47761 ttgggaacct tatgattatt cgattgttta tgcggaacct tatcgcgtac aacagattac
    47821 aagcaattgg cgtcttccat ttccagttgc attagtcaat gcagcaactt ttttgttggt
    47881 cgtaatcttt gaatatttat ttttacgttc atttattggt tgggtcattt ctttttttcc
    47941 accagcagga gttgtttttt atataggact cccctattta tgtgtgttag cactggcaaa
    48001 actaaaacct gatggaaaaa aagtgcattt ttatttatgg gattggtttt gttatatgat
    48061 tcgtatgatg aatggtgatt gtgtgtatta taactgggac aaggtatcaa acaatgcatt
    48121 ggaagaatta ctttttgtga ataagtatgt ttatttagag gattagaaaa agggacaatc
    48181 aactggttgt tcctttttta ttgggaggtg aaattcattg gtgcttgtaa aagaaccagt
    48241 aaaagcattc aagaaaaatt gtttgttaaa tggagaggat tgtatctggg cctactatca
    48301 tgtttttcca cgaacaattt caaaaaacac accaaaaaag acacaggaaa taaaagaaat
    48361 gatggctcgt ttactgttgc ttttagcaga ttatgaagat gtggagttgg tgatgtatcc
    48421 acgaacaatg aatttacgga cgaagtttga cacattagga aagggatttt ctcccgatag
    48481 tcaagcaatt ggtgcgtatt atgcaaaaga aacagtgaca ttgttggaac gagaaggtca
    48541 agtctatcgt ccttcattta ttttaggaat caaagtcaaa gaaaaagcaa cggtggagag
    48601 ttttcatgat gtaataaatc taatctggtt agatttagca ggaagtttag cacgcttatt
    48661 tggattagaa gtagatgatt tggaaacttt gttttctgaa agtgatcctt atgtaaaaga
    48721 agtgacacaa ttactttata gcttttctat tcgtccctta gtaaaaaaag agctgttgta
    48781 tctcaatcgt ctgcaatata ttcgaggaat ggctcacaaa gtagaagaac aaaataattg
    48841 ggaagtactg aaagacaatc aaggaacgta tgtacgtata ccgaaggaac ataaaggcct
    48901 tcttcaatta gaagatgagt tagggagtag ttatttagca ttgttgccta ttggggaaac
    48961 acctgaaaat atgagaaatt gtgatctatt tcaatttgct caggatcttt ctttcccagc
    49021 agaattgcga atgaaaattc actatccacg cttgaaagga cagggaggta ttggtttaaa
    49081 actgaattgg ctatcggaac ggttcaaaac agagcataag gaattggttc aaaatgatga
    49141 agaacctagt gaacgcttat taactgttcg aggattagta aaacatttaa agcaacgagt
    49201 agataatggg caacctgtcg tcgattggat cgcatgtttt attgtatacg gtcaaacggt
    49261 tgaagaagtt cgtcaaaata gtgcaacggt tattcaacaa cttcatacga ttatccatat
    49321 ccaccgtgca aaaaatgatc aacgagaatt gttctataaa tgtttattag ggcaaccatt
    49381 gggacggcag ttacattgga agcaccgaac aactgtagaa gcagttggcg aatggttgtt
    49441 ttcagcagta tcagaattag gtatggaatc cggctggtat attggtcggc aagatacatt
    49501 aggggatggt ggttctcgag gagaaaaaac accattagaa gaattaattt atgcgtcaaa
    49561 tcgttttgtc tttttgaatc ttctagccat tgcggaagga atcaaaggtg cgttgtttga
    49621 tgcgcctcat gtagcagtaa ctggaaaaac cggtaaaggg aagacctttt tagttggctt
    49681 gctctttttg tatagttcgt ttatgaatgt acaaagttta tttgtggatc ccaaaggaga
    49741 aaagcgcaaa tggtttacga aactcattca agacccgtat tatcagaaac attacccttt
    49801 gtttgtagaa catttgcaaa aaatgagtta tgtcacgtta gatgctacaa atccccgtaa
    49861 ttatggtgta ttagatccaa ttgtttgttt atcccgagta gatgcgaaac aagtagccca
    49921 ggatatgatt gatgagttaa gtccgttagg taccaatcac cttttaaaag gggcggtatt
    49981 aaagggaatc caagaagttt tagttcaaaa agaagccgga caacaagttg gcttgatgca
    50041 tgtggtcgac tatttagaac aaatggaaca aaaagaggtt cgagaatatg gtgagttttt
    50101 acgcttgacg gtagaagatt cgattttacg tttaggattt tcttatggag aaaacccggg
    50161 attagacttt aatgccaaaa caacgatttt agagatccaa gatttgaaat taccaaatga
    50221 caatgtccga ccagaactat atacagatgc ggatcgaaag tctttgtgtt tgatgattag
    50281 tttaggtcgt ttttgtgaaa tgtttggaaa acgagatagt agtaagaaaa cagcgattta
    50341 ttttacagaa gcatgggtat ttaataattc gaacgcagga cgtagtatta ttgcagcaat
    50401 ggcacgtgtg gggcgttccc agatgaatca attagtgtta gatacacaat ttattggtga
    50461 cttaggttca gataaagaaa aagggaattt tggtgtcgtc tttgcttttg atgaagacag
    50521 tgagcgagaa aagattttga atcatttacg tttggaagta aatgaagcga acttagctga
    50581 aatgagccac atgattaaag gacagtgttg gatgttagat ccctatggac gtgtaggaaa
    50641 actcaatgta cacagtttgt ttgaagaaat gaccgctgca ttacaaacaa cggaaaaaac
    50701 aagtcactct caagtagaag aacaggatac gttaatcatg caatagaaat gaggtgaaac
    50761 aatggcttat ataaagcata aggaaaagaa atggtgcgtt ttgttttttt taacgattgg
    50821 attcggactg ctttttgttg gtggattggt gtatgcggca gaaaagcagg tggatcaatc
    50881 gatctatgag attgggaaat ttcatattga taccttttct tcttatttat ctgatagtga
    50941 attttcatta ggcaaaatgt tatttggggc aaatggcaca gattttctaa aaatgatgac
    51001 gaatatgttc tttagtttaa gtaaattgat ttggcaaggc cttgattttg tcattgagaa
    51061 gctctatgaa ggagctgcaa tgaacgagtt aattcaccag tttttcactt tttcggatac
    51121 tatttattca aaaatatacg atcctattgg tgcggggatt gtcgtcttgt atgtgttata
    51181 tctatttact cttcgtatat ttagagggaa acattacgca aaaatggtgt ttattcgatt
    51241 tctcttcgtt gtcatttttt caatggtttg gtttggctat ggtagtaacg tacctagtaa
    51301 aggagaagct tttaccaaag ggttaaatga tttgtccgtg gaggtagaag gctttatttt
    51361 ttctgctaca aatagtctag atgaattggc gacaaaagat gtcaatggtg ttttagtgcc
    51421 aacgacacaa aaggaagcca ttcaaaaagt gcgccaactt tattataaag cggctgttgt
    51481 tgatccgtat atattgttaa attatgggac aacaaattta gaagaattgt atcaagcaga
    51541 gattgaacca caagaatttc tagccaagga agcgtcaagt gaagaattat ctgatattga
    51601 gacaaaagtg accactgctg caggagaaga tagagggaaa aagaaggctt atcgtgtcta
    51661 tattcaacca actaaagctg tttataaagc agtcgttggt tctatggtgc cattgcttaa
    51721 tctaagtatt ggattaccac ttgtagtgat tggcatgatt cgttttatct ttcaaatgtt
    51781 ggtgttgtgt ttattacttg tgattccttt ttcattactc gcttcttttg ttccagtatt
    51841 tgattatatg ctcatgaata ccttaaagtc ctttttgggc ttgatgtttc aaaaatcttt
    51901 ttacagtgtg attattttgg tggcattttt gatctataac atagtagacg atttaattcc
    51961 aatgaatact gtttttggtt ttttaggaaa tatgtttgtc aaagcactcc tatcaatttt
    52021 actcattgta aaacggaaag atttattaaa gaaactaggg ttaggagctg cagatcaaac
    52081 tataaacaac acgaaaaaca tagcaagtca ttctttgtct caaatgaaac aactgcctgg
    52141 tcaactatca actgctggac aaaatttggc aatcaaaggt gcaggattag cagggaatgt
    52201 gtatcgagca acgaatccac tcgcttctgc agcgatacat ggatttcaaa agtttcaaca
    52261 aaaacgtaca ccacaagaat cggtaaaagg gaggcaagtg gttaatccct ttgaactagg
    52321 tgggcgttca aatcaagcct tgccttatag tggtcgtcat gttcgttatc atggggtaga
    52381 gcctgaaatg tcttctacca aatcgaaaac agagtggaat tctggtcttt caaatggttt
    52441 gattccaaat tcgtccatga attcagtaag accaaaacaa caggtacaat cattggcaca
    52501 attgagtaag caacaaggga ataatcaaag aaaaccattg ttaaataaag gtatcgcttt
    52561 tgttggtcaa tcgattccgt tgccttctgg aaaagtaccc gtgccacaat taacagctgc
    52621 tggttctctt tcacaacaga ttcgccaagt gaaaaatcag gcgaaaaatg ggagttatcc
    52681 agcaatgcag cagttattga accaatactc tgttcctaat tcgtcggtag ccactctatc
    52741 aggaaatcgt tctgtgcaac aaccggggca acgttcgtct gaaagtaaac cgtcgatttc
    52801 cgcaccaaaa tcgttacaac ctgcattttc ttctttagat aagccaacag agcaatcgga
    52861 aaaaatagaa aaggatccta aaacactgcc gattttggac gcaaaaccag gaactgattt
    52921 tactttacca aaaggacgaa cagtccaaaa accattatcg aagaaaggat gaggaagaaa
    52981 tgaagaaagg gtgtctaggt tgtcttcttt tctttattct tccctttttt ctattgccat
    53041 tctttttttc aggagaactt tctgagcatg gaggagaagc tacaggtcta gctgtcgctt
    53101 ttaatgggga atataccgaa ggattaccga ttttttcaga gattaaagga cgtggtccaa
    53161 tcacagatga acatgcgcaa gcagcagttg gtgcagctgt aaagtatcag ttattaccga
    53221 gtgtgattct ttctcaatta ggctatgaat cagcttatgg acagtcacaa tctggaagaa
    53281 acgatacgaa cttttttggg attacctggt ttcctggttg tccctaccca caaggttctg
    53341 cacgtggaat tggtggttct gaaggtggtt tctatatgaa gtttccgaac ttaaaagctt
    53401 gttatgacta ttatggcttt atggtggcga gccaaagcaa ctttaatcaa tgtgtgggca
    53461 ataaagatcc tggaagttgt ttattgattc taggtcgtgg aggctatgcg gcagcaggta
    53521 ttaccgaagg gagtccgtat tatcgaggag caatgagtat cataacgtcc tataacttaa
    53581 cggaatatga tacttttgcg atcaagcatt ggaatagcct accaacaaaa aaaacttcgc
    53641 ttattgcagg aaaaagtaaa ggcaatgcga aggttttgga tcggatcatt ggacgatccg
    53701 taaataatgg ggaatgttat ggtggtacgg catggtatgt atctcaatta ggtggcccac
    53761 agttaatggg ttctggacac atgtttgcga tgttaatagg ggaagattat aactggacaa
    53821 cctatggttg gcaagtaatc atgaacccaa aaccaagtga tttacaagca ggagatgtgg
    53881 tgaattggca agcaggtggt gcattgtctc ctggaatcta tggtcacacc ggaattataa
    53941 cagatgtttc atatggcgga caacatttct ctagtgtgga acagaatgcc gagcaaggac
    54001 ggattatggc tcggtatcaa cgcacgtaca atcaaacaaa aattcgtagt ctggttcgta
    54061 aggtgaaata agaaaggcag tgactagaaa cgtgaaaaaa gggataattt tagtatttag
    54121 ttggttatgt ttgataggtc ttactggttg tcattggttt tcttctgaaa aacaaaaaac
    54181 acaattgaga acgacagaag aaacaatcga agcagtaaaa gaaacaagtg gaactcttca
    54241 aagtactgaa agtaacagtc aaattggttc attagaagac gagacagcgc aacaaatgca
    54301 aatagatgaa atgacaaaaa atttcttaaa agattttgct agaaaatgga gtaatttcac
    54361 agatgtttat aaacgcaatc aagctgtgag ggactatatg acagatcgtt gtattaaaga
    54421 caatagtatt gatgtagacc cgcatgtaca aatgaaaaca actgggaaac tttaccaggt
    54481 gacacaagat atagagaatc cggatcaata tttgttattt ggagaagaaa caatgaataa
    54541 ggttactcaa tttgtattac ttcaattgaa agtagatcaa gaagctcaaa agattgatgc
    54601 gatcaagatt tattatgtac ggagtgcgta ttaaaataaa aaatgaacac tcatatttat
    54661 tatacaccga agaaatagaa aaaaacagac tgttttttta agaaaaacta tgtcattctt
    54721 tacttgaaaa aagttgttat aggacagaaa ggataaaaca aaaacggaaa aaatatgtct
    54781 tatgaataac tataagaact attctatttt ttaggagatg gatacgagat gaaaaaacta
    54841 attggctata gtgtgatgtg tattggattt gttggtgtgg gattgtttgt atcgactgaa
    54901 tctgcttctg cggctgaatg gtataatcct atctggtata atccgaacat tgaatgtcaa
    54961 aaaggctatc cgtatggacc gacaaatctg aaaaatggaa aatgtagagt aaattggaat
    55021 ggcgttgtag atagtgtaat tactggttca atggactctg ctctacatgg ctttacgggt
    55081 cactagaagg aggaagaaaa tgtctgaaga gaaaaaagaa atcgttgaat cattagtagc
    55141 tttaaaagat tctctttcta agattgaata tgttgatagg caagaaattc aaaaactaat
    55201 tgatgatact attattgaaa tccaagatgc gcgttgtgaa ggtataaaaa tttctgtagc
    55261 tttatctaaa gtaatagaaa aaatgaaccg ttcattagcg tttaatggat tgaagctaga
    55321 tagacaaact agtctgatat gggatcattt aaaagacttg tatgacaagt ctaaaagaag
    55381 tgaacggact gctgtatcaa tcttaaaagg cctttgggga atgaattcat
//
LOCUS       DANKBF010000009        55283 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000009 DANKBF010000000
VERSION     DANKBF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..888
                     /locus_tag="PWY89_000586"
     CDS             <1..888
                     /locus_tag="PWY89_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL2065645.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(938..1240)
                     /locus_tag="PWY89_000587"
     CDS             complement(938..1240)
                     /locus_tag="PWY89_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2065646.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(1265..3358)
                     /locus_tag="PWY89_000588"
     CDS             complement(1265..3358)
                     /locus_tag="PWY89_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2065647.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(3847..4611)
                     /locus_tag="PWY89_000589"
     CDS             complement(3847..4611)
                     /locus_tag="PWY89_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL2065648.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(4722..5591)
                     /gene="mdcB"
                     /locus_tag="PWY89_000590"
     CDS             complement(4722..5591)
                     /gene="mdcB"
                     /locus_tag="PWY89_000590"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL2065649.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(5616..6692)
                     /locus_tag="PWY89_000591"
     CDS             complement(5616..6692)
                     /locus_tag="PWY89_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL2065650.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(6716..7309)
                     /locus_tag="PWY89_000592"
     CDS             complement(6716..7309)
                     /locus_tag="PWY89_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL2065651.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(7313..8989)
                     /gene="mdcD"
                     /locus_tag="PWY89_000593"
     CDS             complement(7313..8989)
                     /gene="mdcD"
                     /locus_tag="PWY89_000593"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL2065652.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8979..9278)
                     /locus_tag="PWY89_000594"
     CDS             complement(8979..9278)
                     /locus_tag="PWY89_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL2065653.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(9380..11026)
                     /gene="mdcA"
                     /locus_tag="PWY89_000595"
     CDS             complement(9380..11026)
                     /gene="mdcA"
                     /locus_tag="PWY89_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL2065654.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(11033..11944)
                     /locus_tag="PWY89_000596"
     CDS             complement(11033..11944)
                     /locus_tag="PWY89_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2065655.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12064..12921
                     /locus_tag="PWY89_000597"
     CDS             12064..12921
                     /locus_tag="PWY89_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2065656.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(13108..13470)
                     /locus_tag="PWY89_000598"
     CDS             complement(13108..13470)
                     /locus_tag="PWY89_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL2065657.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            13816..15255
                     /locus_tag="PWY89_000599"
     CDS             13816..15255
                     /locus_tag="PWY89_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065658.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(15262..17814)
                     /locus_tag="PWY89_000600"
     CDS             complement(15262..17814)
                     /locus_tag="PWY89_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2065659.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17928..19259)
                     /locus_tag="PWY89_000601"
     CDS             complement(17928..19259)
                     /locus_tag="PWY89_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2065660.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(19225..20703)
                     /locus_tag="PWY89_000602"
     CDS             complement(19225..20703)
                     /locus_tag="PWY89_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2065661.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(21008..22843)
                     /gene="tdc"
                     /locus_tag="PWY89_000603"
     CDS             complement(21008..22843)
                     /gene="tdc"
                     /locus_tag="PWY89_000603"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2065662.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            23313..24740
                     /locus_tag="PWY89_000604"
     CDS             23313..24740
                     /locus_tag="PWY89_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065663.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(24832..25197)
                     /locus_tag="PWY89_000605"
     CDS             complement(24832..25197)
                     /locus_tag="PWY89_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2065664.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(25194..25559)
                     /locus_tag="PWY89_000606"
     CDS             complement(25194..25559)
                     /locus_tag="PWY89_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2065665.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(25751..25999)
                     /locus_tag="PWY89_000607"
     CDS             complement(25751..25999)
                     /locus_tag="PWY89_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065666.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25998..26126
                     /locus_tag="PWY89_000608"
     CDS             25998..26126
                     /locus_tag="PWY89_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL2065667.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            26183..27877
                     /locus_tag="PWY89_000609"
     CDS             26183..27877
                     /locus_tag="PWY89_000609"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL2065668.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27881..28000
                     /locus_tag="PWY89_000610"
                     /pseudo
     CDS             27881..28000
                     /locus_tag="PWY89_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27977..28150
                     /locus_tag="PWY89_000611"
     CDS             27977..28150
                     /locus_tag="PWY89_000611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065669.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            28528..29661
                     /locus_tag="PWY89_000612"
     CDS             28528..29661
                     /locus_tag="PWY89_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL2065670.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            29692..30012
                     /locus_tag="PWY89_000613"
     CDS             29692..30012
                     /locus_tag="PWY89_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL2065671.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(30041..30505)
                     /locus_tag="PWY89_000614"
     CDS             complement(30041..30505)
                     /locus_tag="PWY89_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2065672.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(30637..31890)
                     /locus_tag="PWY89_000615"
     CDS             complement(30637..31890)
                     /locus_tag="PWY89_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2065673.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31919..32236)
                     /locus_tag="PWY89_000616"
     CDS             complement(31919..32236)
                     /locus_tag="PWY89_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2065674.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(32262..32582)
                     /locus_tag="PWY89_000617"
     CDS             complement(32262..32582)
                     /locus_tag="PWY89_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2065675.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(33046..34461)
                     /locus_tag="PWY89_000618"
     CDS             complement(33046..34461)
                     /locus_tag="PWY89_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2065676.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(34478..35794)
                     /locus_tag="PWY89_000619"
     CDS             complement(34478..35794)
                     /locus_tag="PWY89_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2065677.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35944..36642)
                     /locus_tag="PWY89_000620"
     CDS             complement(35944..36642)
                     /locus_tag="PWY89_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL2065678.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36931..37887)
                     /locus_tag="PWY89_000621"
     CDS             complement(36931..37887)
                     /locus_tag="PWY89_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2065679.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(38000..38833)
                     /gene="msrA"
                     /locus_tag="PWY89_000622"
     CDS             complement(38000..38833)
                     /gene="msrA"
                     /locus_tag="PWY89_000622"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2065680.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(39008..40642)
                     /locus_tag="PWY89_000623"
     CDS             complement(39008..40642)
                     /locus_tag="PWY89_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065681.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(40629..42497)
                     /locus_tag="PWY89_000624"
     CDS             complement(40629..42497)
                     /locus_tag="PWY89_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065682.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            42463..44100
                     /locus_tag="PWY89_000625"
     CDS             42463..44100
                     /locus_tag="PWY89_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065683.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(44245..44985)
                     /gene="truA"
                     /locus_tag="PWY89_000626"
     CDS             complement(44245..44985)
                     /gene="truA"
                     /locus_tag="PWY89_000626"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2065684.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(45176..45811)
                     /locus_tag="PWY89_000627"
     CDS             complement(45176..45811)
                     /locus_tag="PWY89_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2065685.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(45958..47199)
                     /gene="sstT"
                     /locus_tag="PWY89_000628"
     CDS             complement(45958..47199)
                     /gene="sstT"
                     /locus_tag="PWY89_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2065686.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            47397..48395
                     /locus_tag="PWY89_000629"
     CDS             47397..48395
                     /locus_tag="PWY89_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065687.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            48408..50426
                     /locus_tag="PWY89_000630"
     CDS             48408..50426
                     /locus_tag="PWY89_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL2065688.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(50486..51202)
                     /locus_tag="PWY89_000631"
     CDS             complement(50486..51202)
                     /locus_tag="PWY89_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2065689.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(51429..51971)
                     /locus_tag="PWY89_000632"
     CDS             complement(51429..51971)
                     /locus_tag="PWY89_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2065690.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(51976..52203)
                     /locus_tag="PWY89_000633"
     CDS             complement(51976..52203)
                     /locus_tag="PWY89_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2065691.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(52282..54162)
                     /locus_tag="PWY89_000634"
     CDS             complement(52282..54162)
                     /locus_tag="PWY89_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2065692.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(54351..54965)
                     /locus_tag="PWY89_000635"
     CDS             complement(54351..54965)
                     /locus_tag="PWY89_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2065693.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            55107..>55283
                     /locus_tag="PWY89_000636"
     CDS             55107..>55283
                     /locus_tag="PWY89_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2065694.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT        17030 a        11127 c         9178 g        17948 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc agtttttacc ctcttttctt tttgtattta gataactccc ttatcttgca
      961 gaactggggt gattttttct tcaatttcat tttttgaaac aatatttttc aaatagatca
     1021 tcgccgtttt aggatcaact gcgaactttt cgaactgcga acgaaaattt tctgctgtga
     1081 tgatcacatc tggtttcatg gaaactgcgg aagaaatatc acaatggtcc agtttggctt
     1141 ctactcctaa tttgttcaaa acatcctcag cagacatctg tgctgcaaaa ctacttccta
     1201 aacctgcacc gcatacgaat aaaatgttca atttgttcat ctttgttctc ccccgtatca
     1261 tttattattt ctcaactact gaaaaaagca gttgtttgaa atcttcgata tttgtgcatt
     1321 caatcaatcg atccaacttc tcttcgttgt tgatcaattc aattagttct ttcatgatgt
     1381 tcagatgcga gtaggagtct actgctgcca aacaaaaaat aatctttact ggatccattt
     1441 caggattacc gaaatcgatt ggttgctcaa tcgtagctac gctgactcct aatttattga
     1501 ccccatcttc tggtcgggca tgggctagtg ccatgtgttt gccaatcacg atatacggtc
     1561 cgtaatcatt cactgcacga atcatcgctt cgacatagcg ctcttcgaca atgttttctt
     1621 taaccaatgg tctagcagct agttcaatcg attcctccca agtagtggct ttttctttga
     1681 tcatgatatt gctgtccgtc aaaatatctt ttaacatagg ctggatctcc cttttgttga
     1741 tttctaaatt gtttgtttcg aataattttt ctaacttgcc atagatgatt cgatctactc
     1801 ggccaccact ttctttcatg acatctatta tttgagtgaa taactccgtc gattcatgac
     1861 tgttaggctc taaccgctgt agttcttcat ttcttttcaa aaagtcttct ataaccaaga
     1921 tattcgtctc agttaaaatc ggatcgacaa ctaaaagcgg cttatttcga taattcaatt
     1981 gaaaggtgct gaaaaccaga ttgacatcca atttatctac taattcgatt tcttttgaac
     2041 ttaaaactgc agtaatctcg atctgaggaa acttttcttt cagattctct gctaacagat
     2101 ttccagtagc catcccatgg ttacagacaa cgactgattt ataaacataa ttcaagtctc
     2161 gcttgattac actggcgatc gtcgaaaagt ggatgacaag aaaagctatc tcgtcctcca
     2221 gtatcttgcc accaattact tcttcgatcg taggaacaaa atgagtgact gcgcggtaga
     2281 ttgggaaata atttgttcgg atgttttctt tcaatggatt catgacttgg atatgattct
     2341 tgattcttac aatcagtgca gccatgtgct tatagagatt ttcgaataaa gcttcttcct
     2401 ttagatggaa aggtacgtgt gtctcgttct ctacaaattg gatcaattgg atcgttagta
     2461 attgagcttg tacccactcg attgaattgg agatatctct agcattgaac gtctcaatag
     2521 tgaaacgaag ataatttctt tctacttgcg ggacattgtc caattgaaat tcctcgatca
     2581 tgtggtcaat aaatgcagaa aagctactgt cttttatttc tgtatttttc caattaatgg
     2641 cagagataaa ttcatgtttc ctgattcgct ggatccagat caatgtaaaa agcaggattt
     2701 gttttttata aatatcatct tcttgtttca acaacttatt ggaatagatc tcctgtattt
     2761 ttttgatttc caccaaagga aaataatgga gaaagatttt ttgagccggt gttaattgtg
     2821 catcttgatt cataaagaag tcggtcattc ccagattctg attgataaaa tcatagatca
     2881 tcgtacggat agatcgttct gaacctttat aaaccagtcc ttgctttcca taactgacga
     2941 tctctatccc ataactgacc agtgttgctc gtaaccgtct catatcttca tctaatgtac
     3001 ttttagagat ttgaaattct tcttcttttt tgtttagatg gatcgggatt cggctgtaag
     3061 caacggctaa aactagatca aaaatacgtt cgcgccgatt caaaaagccg tctttcacat
     3121 cttttatttc agtttctaat cgagcttgct tttccttttc cagcttcaac aaaaaacctt
     3181 tgttgcgaat tgtacggatc tccggaagct ttttactgct caaccagata ttcgcttcaa
     3241 gaacttcctg acggatcgtc cgctgactga cattgaactc acttgctaga tgttttgctt
     3301 ttactggata ttgggctgtc atgattctgt gaagtatttc aattgttctt gttttcatag
     3361 aaatgatctc cttctgattt caagtatatg gttccgctta cataataaaa agtagatatt
     3421 tctgccactt gaagtggaaa gaaagtaagc aaagcaaaca aaaaataagc catatgcttt
     3481 ttcatctcct caacgaaaaa gacatacgcc tattattaca aaaattttgc ccatctatac
     3541 aaaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacaact aaactaaaat
     3601 agtgcttcct atttttcgtt tggttgttct tattatcgca gatgcaagct tttaaacacc
     3661 atttagtgga gaggttgtga cagagatgtt cagccttgag cgataagaag cgattttgaa
     3721 aaatcgcttc ccatatttta caaaatcgtg ccttattgcc gataggctat ctctggcaca
     3781 ccgtttattc agaaaaagga gctgcgacaa ggcttttgtc acaactccgt tcataaatat
     3841 ttcctgtcaa tccagcataa tcggctgaat aaaatcggct tgccatcttt tttctttctc
     3901 agaaaaatca aaataccata tctgagaaaa atactgtcgt tgtgtcttac ttcctgagat
     3961 caatcgccca tcttttcgat cgactgaata atcacagcat ctgaaatcaa ttcggatggc
     4021 aaaactattt tcgtggatct gtcgataatt tcctacttcc tgaatagtta tttttttagt
     4081 atgatttctt acaccgtctg ctttgttttt ttgcagaact ttctgatgtt cggcaaacag
     4141 cttgtctgta taacagcttc tggctttttc aggagtttct tcttcccaag cggattggat
     4201 cttcatgaat atcttttgga tttccagaaa ctgtgctttc ttttctttag ccgttcctga
     4261 catgcgggac aatatctccg ctttcagcct tttcttttgt cttttattct tgacgataga
     4321 aaaagcagaa acccctccta ctgacaaaac agctaatgca agaaatcccg tttgctcata
     4381 actagaagga cctgtacctg gaccataata cccataagaa tctctgccgt aagaacgact
     4441 gccaaaataa ctgcccccat atgaacgacc accaaatgaa cctctgcccg aacttcgacc
     4501 gctagatgta cctccaccat gaccacccgc gacagcgaat ccttgggtcg gcagagcaaa
     4561 caaacatagg cccactaaca aaatgattgc taaataattg atttttttca ttgtcttcct
     4621 cgttatcttt tattacaaaa aactattttt atgatagcct tgctaaaagg aagagtcaat
     4681 aaccagatgc ataaactgtt tttttagcaa ttttgtctta tttagtcatt gagtaacatc
     4741 ggacaaatgt atgtgtcggc agtccataat tcctctatct tcagcatgaa caagcttgca
     4801 gacaagagat ctgcgcttcc tccaggagag atttggtttt cctgacaaaa gtgcaacagt
     4861 gctgtaaatg gacgatttgg taaagcactt tgactagcag catgagcaag tttttgcatt
     4921 tccactaacg ttttctgatc actgcgatgc aatacgcatg tgtcatctag cgaactcatc
     4981 aaaactaaca acatatgcat ttgtttctgt acatatgaat cattatctag ctccttagct
     5041 gcttgaattg caagcctgat atgcggatac cctaatatcg cttcttcata tgcaccattt
     5101 acgccatatt tttgcttcac cctatgacca tgagtggttt ttttatgacg atattttgta
     5161 tcaggaaaag cagcaagctt gctgacatca tcaagtattt tcgataaaga aaaaggctgt
     5221 tccatactga ttttttgtgc aatgacactg atcaatagcc ccattgccca aatcgctcct
     5281 ttatgggtat tgatgccatt tgtagcaatc agcattgcct tttccgcttt tcgcccaatc
     5341 tcagcaattc tttctcttaa ctcctgatca atagcatgat gccatgctat ttgagccatt
     5401 tcttcaaaaa aaggagttag cgtcttagca gacttaagaa ataaagcata gtccatatcg
     5461 gaatgtgcac ccgcatccag tgcatccact aatccaggtt ttggacttaa tgttacttct
     5521 gcgatcaatg cttcctctac caacgcagcg agttgctgag tcactgcttt ttttcgttcg
     5581 tcatttatca tgacactcac tccttcttcc tactattact tgctgaaacc ttttttgata
     5641 gctacccaaa tgatatgaag taaccagctt aaagcaaatg cacaacttgc cgtcattaga
     5701 taagctacaa gaatcatgac tcctgaaggt aactgcagcc aactatcttt aaacaataat
     5761 aacagtactt ggaaaacaaa tacattggct aaatacgtac gataagcata aacagacaac
     5821 catttgaaca atggcaattt tggcgaatcg tttacaatca ttttggaagc gcctatgaag
     5881 acagcaaaga tgatgactaa actgtataaa ctttggatcg tattcaagta aggtgcattc
     5941 gcaaagctca agtctcctgg atgtgcgagc aatgaacgga cagaaaagaa cgcgataact
     6001 aaggcaacag gtaagaaaag ataacgtact ctgtccaaat attgatagac tttttcgtga
     6061 taaacaaatg cggctacccc ataaatcatg aaactagcaa caaaccgatc taataagtac
     6121 cagctgtcat gataaggccc agtcaatact tgtgtttgat agaacagata ccatgcgaca
     6181 tataaaatcg ttgaaccaat caatactggc catacatatt ttttattagc aaatactttg
     6241 taaccgagcc aaacaaaaaa cggcatgaac aattggatct gcagcatcat aacggtatac
     6301 cataaatgag gcgctccatc acctgtgaca tatttcagta aaaagcttgc agtatccgta
     6361 tacggagttt gttgttggac tgctggaaat agccaaagat aagccgtcgt ccatagaaga
     6421 tacggaacga ctagttcaaa aaacttttct ttcaaaaatt cccaataaga caaatgctga
     6481 cttgttttta ccatattata aacaattgca aaaataaaca ttggtgctgt gtatttaacg
     6541 atgacgtaaa aggtaccaag aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca
     6601 aagctcaata tcgtttgcat cataacagcc gttacagctg atactttctg gaagtcactt
     6661 acgttgattt tgattttttt gttttcaaac atctctttct ctctcttttt ctttattacc
     6721 aaagttcttt ggttaattta ggtccttcga ctgttttcaa tagaattttt ttctctggct
     6781 ctctcaaata ctcttttagt gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt
     6841 gctcaaaaaa agcaggatgc gattgaatta cctgaattgg caattcaatc ggtgaatttg
     6901 cgtacagcag caaatcaaaa tcactggttt cttttactgc cggaatccca gtcgataatt
     6961 caaatcctaa gctgccgccg attccccatt tacatcctga taatatgttt ctagcagcat
     7021 gaaattgctg ataaacagga taatccatga ttttctcttg tctatacgat tcttgttcga
     7081 tgatgtccca cggatgaatg acttgtttga cggcatgttt tgagatttct gttgaccatc
     7141 tctgattgcg ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg
     7201 cacgacgaac tgttccgaac atcttttcag ttagaacgac ccattcaggc aaggtgaacc
     7261 cttctatttc atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat
     7321 cccattcttc gtccattttg gcacggactt tatttgtttc gacacgtccg aatgttttgg
     7381 ctgtttcgtt tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa
     7441 tggcttcatt gattgcttcg atgactttgc tgacattttg ttctgaagca gcttggtcgg
     7501 tgatatcttc taggaaatga tagatcgctc ctagttttgt ataattattg atatcgtaag
     7561 cgatagctgg aactttttca gcagctgctt cgatttcagc aaccgaacgt tttgtcacac
     7621 gtgcagcact ttctttagac atcgcttgga cggtaatttc tttattgttt aaagcaatca
     7681 agcggcttga ttgtaatcca tgtgctaaaa atgcacctga aatagcattt cctgcaatca
     7741 atccaataac tggatgtcca gcttgacgga gttttgcata agcagcagca ctattagcca
     7801 aagccacatg gataccgatc aattcttcgt tatagccgta tgcttggctt ggtacatcga
     7861 tcacaacgat gattggccgt ttcacttttt tgtctttatc ttcttcgtaa acgtgatcca
     7921 aaatttgtcc catgcggaaa ccttcaacta aaccaacttc accgtttcgt acacggtaca
     7981 tcgggttttt aggatcaggt acgattgatg tcacgatggc ttcttctatg aatttacttg
     8041 atttaccata atacacagta ctaatagaag aagttggatt tttcatgcca gttagtgccg
     8101 caaaccaatc gtaaccaaca cttgctgcag ctggttcatt tccttctgct acttctggta
     8161 agtcaattga ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca atggccattg
     8221 gttttgaaag atctaaagaa tcaagtaatg ataaataaaa tgctgagctt tcactgcgat
     8281 gcgtgtcttt tcgttcgtcc attgctgcga tgacagcagc tttgattgct tctacactat
     8341 cagtcactaa ttcatcaatg atttttgatt ctacccgttg acgagatcct aatgtattcc
     8401 aaatcaagtc tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt
     8461 ctggtccatt caaaccgact ctcgcttttt ttgttgcgat taaataactc attaatgctg
     8521 aagtaatcga cataccgcca aatgatccta cacgtcctgg aatcaaaccg atgactggta
     8581 catattgttt tagagcaatg attacatttc cgatttctga aatagataac agtccgtagt
     8641 ttgcttcttg caaacgaaca ccacctgtat ctagtacaat gactggaaag atctcatttc
     8701 cttttttatt ttcttctaac acatgttcaa gtgcagcaac gatttttgca ccggaaactt
     8761 caccgatacc gccaccttgg aattttcctt ccatagcgat tactactgct tgtttgccat
     8821 tgatcgtgcc acggctgaca acgatcccgt catcactttc agggacgatt ccttgtgctg
     8881 gtaaacttgg cgcgatgatt tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta
     8941 gagcgttcac tcgttctctg gcgtttaatt ctacaaaact atttttcatc tgaaagttcc
     9001 tccattgctt gcgtcaatcg taggtacaca actcctggtg tagcaccgaa gtcattgatc
     9061 acgatatctg ctgaaattgg ataacgatca aaaaatctcg ttaatacatt gttccatact
     9121 tcgccgaagc catcgcttcc tgtacaaatt tttacagtag attctgtttt ttctgttgga
     9181 aacatcaaga tttccaaatc tcctgaaccg accactccta catggatttt cttattgatc
     9241 ggctgtttcg ttggatattt ataagctaat tgttccatgg atctctcccc tttttgtaaa
     9301 acagagcata cacgttctgc ttattcgtct ttcttcttcc atctgggtaa atggcgacaa
     9361 aaggtttttc tcgccaattt tatgaccaag ttctgaattt cattggtggt tcatacaggc
     9421 catctgacca ttcgatcaaa tcatcgatgg tttgtgcagc taataatgaa cgtttcgctt
     9481 cactacgttt gatgcctaga tcttctggta atttcacgat gccggctgca cgcaagctat
     9541 ctagtgattc tttcgtgcta gtcattccaa gaggtgtaac accgccaatt gctgcaattg
     9601 ctgcttgtcg ttcttctttt gtttttgctt tgtacaaata agcaattcct tcttctgtca
     9661 cgatatgtgt agtatcttcg ctataaatca tcactggtgc attggctaag cctgcttgtt
     9721 tttgaacagc tagtgcatct aattcttcga caaagactgg ttttttattt gaaccatatg
     9781 tttcaaccat ttgaacaact aatttttggc ctttccctaa tggatcatca ttgtcgcgaa
     9841 gtgattgcca tgctggtgta gaatgacgac gaccgccagg attatgtccc atgttaggcg
     9901 caccgccaaa accagataaa cgtccttttg ttacggtaga agagtttcct tcgtaatcta
     9961 attgcaaggt tgaaccgata aacatatcga tggcatattg tcctgcagct tgtgataatg
    10021 ttcggttact gcgcatcgtg ccgtctttac cgacaaaaaa gacatctggg cgagcagcaa
    10081 tatatttttc cataccaact tcgccaccaa aactatggat gctttcaacc caacctgatt
    10141 cgatggctgg aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg attttccctt
    10201 ttaaccctag tgattcacca taagttggca gtaacaattc gatagctgct gtattgaatc
    10261 caataccgtg attcaaagat tgtacgccgt gttctgcgta gattcctttg attgccatca
    10321 ttgccattag aatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta
    10381 attgataagg tttatctgct ttcacgacaa cgtctaccca gtcaccaggg atatctacac
    10441 gtggtaaaga atcgacaact tcgttaactt gaacgatcac gataccatct ttgaaagctg
    10501 cagcttcgat gattgttggt gtttcttccg tattgaaacc tgtgtacaag ttgccgtctt
    10561 tatctgcttt atctgcagct actaaaacaa catttggaat caaatcgatg aataagcgtc
    10621 cataaagttc caaataagta tggatatcac ctaatttcat tgtgccgtct tcaatcattt
    10681 gggcgacacg caagctttgt gcaccagcat aagagaaatc gatcttgctt gcgattcctt
    10741 tttcaaaaat attcaaatgt tctggtcgtg agatactaga catgatcaaa tgcagatcat
    10801 tcactttttc tgcatctact ttttcaagac tttctgaaag aaaacttgct tgtttttgat
    10861 tgtcgccttc taatacaact ttgtcgccgg gagcaatcag tttttccatt acttctacga
    10921 ttttttcagt ttctacatac ttgccattga gcaagttgct gattttttcc aatcgctgcg
    10981 cttttttttc gcgtttagaa gcccagttgc ggtttggttt ttccataatt agtcatctcc
    11041 atttttgata tagaaacgtg gcgtcatcaa taccatgttg gtctagattg atcgtacgga
    11101 tccctttatc tacaccaaat tttgtatgaa tcagctttgt caaagtattg ccggggggaa
    11161 attcaatcgt cagatccatt cctaattctt ttgccaagtc catcatttgg ttccactgca
    11221 cagggaaagc taggttttcc aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc
    11281 ttccatccgt attttttata tacaaacatc tagcatcaga tagatggaca gactgcatat
    11341 acggcttgaa tttcttgacc gtatcattca tcaagagaca gtgagatggc acagggactt
    11401 tcagcagctt cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta acggataaac
    11461 cttcgatact cccagataac gtatgttgca tcggacaatt ttcatttgac agataaactg
    11521 gatggtcagg atcaaatgtt tttgctacaa tttcctcagc ttcgcttctc gtcacaccta
    11581 ctactacgcc cattgcataa ccgcttggat aagcttcttt cattaagact gcgcggtgat
    11641 agactagttc cgctgcattc tcaaatctca aagatcgaca agcaacagct gcacaaaaag
    11701 caccaatcga atgtcctgct gcaatatcag gctgaatacc gtttttttcc atttcatcta
    11761 aataaaatac tgcttttgtc agtaatgcta gctggataaa aaccgtatct tgatagtttt
    11821 cttctttatc aactaaccga taccccgtca cattttctaa acgttccatg tacatttcag
    11881 ggatatccgc taacatccct aacttctgac tgccttgtcc aggaaataag aaacctgtac
    11941 tcatgagcct cgcctcctca gcacttactt tttccactgc tatcttagtc aatttctgct
    12001 ataataaaaa atgccaaaaa aagaaaaaac cataacaaaa aggtatggat gggatggata
    12061 attatgaaat tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc
    12121 caagcagcca aagaattatt tatttctcaa cctgccttga gccgtgcgat caaacatttg
    12181 gaaaacgaat taggtgtccc tttgttttac catagcggca gaaacattga gacgaccgtt
    12241 tatgcagaag aattttatcc atatgcagtc aagacattag acacattaga agaaggaatc
    12301 aaattattgt cttcggtcaa tgagaagatc gttacttccg tcatattgta tttagaggta
    12361 gcctctgttt ctattccgaa cttggtccga atttttatgg aaaagcatcc tgatatccaa
    12421 ttaacgatca tgcaacatca tttaccagat gatattaccg atcctgtact gtacatcact
    12481 tcggagatga agcctggcat gaccaatatt cctatcatga aagaacctgt ttatgtcgcc
    12541 attcctaaga aacatcctct tgctaaaaaa gaacatctgc aattagaaga tatccaacgc
    12601 acccccatct tgatgctgtc aaagagcaac gccttccgtc atacactgga tgcagcactt
    12661 gaagcgaaaa gtatcgaatt gacgatcagt tcgacgacag atgatcctgc gacattacgt
    12721 tctattgtca agcaaggatt agctgttagc tttttcccaa aagtttcttg gtcttacgat
    12781 aaaaatgatc ctttcgtctt gcgggaaatc aaagatttgc ccttgacacg aacaatttat
    12841 ttatcttcta gcttcacaga agatcatcct ttgatcaaaa tgattgcaaa aacgatgcgg
    12901 gaattttatt taggaaaata aaaaaaaaag agctgggaca aaattcttgt cgcagctctt
    12961 ttttcgaaca aacggtgttc cagaagtaat ttcttagaaa ataagccgaa atttcccaaa
    13021 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctaaac atttctgtca
    13081 caacctcttc tctattgtcc tagacattca ttgcgttgat ttaaaacttc catcaatcaa
    13141 atctgctagt tcatctagct tttctatttt acgtagatca atagagatcc aatgttcttt
    13201 attcatgtga taggctggaa aatacccctc ttttcctcgc aaaattccgc caagttcagg
    13261 atcgaccttc aaatcaagaa tattgatgat cctatggtca cttgcatcat catagatttt
    13321 ttcttctgga acagacatca ggaccccaaa ccattttttg cttttttgat gtctgaatac
    13381 gcaataatca ggaaattttt taaaaatata ttctggagac aaattgtatg tttcattgat
    13441 gtgatcgatt agttcttttt tcgttagcat ctgcttccat ctctttctta ttgattcgct
    13501 ttattcaaag ataccacgta agaatagaaa aatgaataca agtagttgct aatcaaatac
    13561 ttcgatattt ttctttttta tcaactctta aatgttcaat ccgcctgtgt tggtcttgct
    13621 accatctact attaaaatgt ttattattgt ttgcccacct attttttgat tttagtggta
    13681 tcatttttca agttcatgat gaaattctta tcatcagtaa atgctccaga aaaataaaca
    13741 atgattgaaa aatcagttga aacttgacca ccgtttattt tctccgtatt cttagaatag
    13801 aagggagtct gattgatgta ttcactaatg aaaaaaatca ttaccgaaaa agatattcga
    13861 cgtcatgttt ctttagtaga acaattgctg aatcatacaa agataaccgt aaatgaatta
    13921 gctgaaatca tagggacaac cgaacgaacg attttttctg accttcaatc tattcgctct
    13981 cagctgcccg aaggctggga catcatcagc gatcaagcag gaatcagttt gcaaaaccag
    14041 caaaatttat taacaaatga cctttgggag atttttttca aacaatcagt cagtgtcgaa
    14101 ctgctgaaaa atctgctttt tacaaaaaaa gttgctgtcc ctgattttct agccgaccat
    14161 gggctttctt atgggacatt gaaaagacat gtgactaaaa tcaatcagcg tttagcaagt
    14221 tacgacttgc agatcgattt gacgaaatat acagcttgta ttcttggaaa agagcgtgcg
    14281 attcgtactt tttatcatcg attgctgatt cctttcactc ataacaacta ttttttcgag
    14341 gattattcga tccatgaatc ccattatttt caatttttaa gaaacctcag tcaaactgaa
    14401 ctagcggttg aaacagaaga gattttcgga acatgttggt tctttatcaa tacgatccgt
    14461 ataaaagcga attgccgatt agattcttct atccatacca acagcacact ctcctccctg
    14521 tatgactctg cattgaaaaa attgtattta acggaaggta tttatcttaa agacactgag
    14581 ctttcttttg cttctttctg ctttctggaa agttggaatt acaacaataa ctatggacaa
    14641 gaaaccgctc gctgcctgca tcattctcca tttttagagg tcctcgaaac gtttgtagag
    14701 gaactagctt cagagctgtc tcttgatcaa ctaaaaaaaa catctctgac tgataacttg
    14761 gctctcctca tattaaaata tcatgaatcg ccgatattga ttgaagcatt ggaccgtcaa
    14821 taccatcgtt ttttagaaac atatgatcag caatatgcac acttgtatca acagaaagag
    14881 aatttactga acgatctaaa aaaagaaata gaaatcaatg aaccaaatta ctttctccaa
    14941 ctcttgtctc tactgatcca acaaacgatt ttttctatca aaccgaattt gttcaatgtc
    15001 tattttattt tccaaggaga accttcatgg aaggcgttct tgcagcagga attaaaagat
    15061 tacttaggaa aacgtgtctg cttcttacct atcgaactaa ccgatctttc cactatttct
    15121 tttgaaaaaa cagatatctt gatttccaat ttccctctag attctctaga tatccctatt
    15181 gtatacattt cttctattcc aacgaaaaat gagctcaatc gtttgactga actcactttc
    15241 aaaacttttt tatagtaacg gctagcgact gtattctcta gctgtttttt cttttttgcg
    15301 aaacagtttg ttcatgatcc ctttttctat ttctacaact aagaatagaa gaatcacatt
    15361 tgctccaatc aataactgtt gtgtcatcgt taaacttact gtaccgatca gctgctggcc
    15421 gactggtaga aaaataacca tcccttgcaa taaagcaagg atacctaatg aaagaaaaag
    15481 cgctttgttt tgcaaaagac cttggttcaa tgacggcttg gacaactctc tgcaattgat
    15541 catgtaaaca gcttgagcta agacgatatt ttgcagaaga atggtttgct gtaagctttg
    15601 cccttcaaac tgcatagcca ataaataggc aggaaccatg atcagtaggg agacatagat
    15661 gatccgaaac aaatgataac cagataggat tccttgcttt ggatctcttg gtgggcgctt
    15721 catgatttct ggatctggcg actcgaaccc tagtgcataa gataaagtga ttgtcgttac
    15781 catattgacc cataaaatct gtactgggga caaaggtagc gggcgattca gcataagtgc
    15841 ccaaatcaag atcaatcctt gagctaatgc agttggcaag aagaaattga tggttttttg
    15901 caaattatcc aaaatccgcc ggccctcttt tacggctttt gcaatcgtat ggaaattatc
    15961 atctcctaaa accatatcag ctgcctgttt gcttacttca ctacctttga ttcccatagc
    16021 aattcctaca tcggatcttt tcaaagcagg tgcatcattt acgccgtctc cggtcattcc
    16081 gacgatttct ccatttttct gcaaagcttt aacaattcgc agtttatgtt ctggtgtcgt
    16141 tcgagcaaag acatcgacta ttggcacttg ctgaatcaac tcttcatcag acataagatc
    16201 caaatcgatc ccttctaaaa ctttggcagt atgttttaag ccaatttgtt ccgcaatcgc
    16261 ttttgctgtg tctttgtgat cccctgtaat cattttcact gagatccctg cttgttggca
    16321 ttcctttaca gctttgactg cactttcttt tggcggatcg atcaatccgg cgatccctgc
    16381 aaaagttaat tgggttaaag tctcatgcgt gagttcctgt ttcgaatcta cttttttata
    16441 agcaaatcct aaaacacgct gaccttttct cgcaaattct gcagctgtct gactccatgc
    16501 ttcttgttct gcagaagaaa gagttgattt agcaaataat acttctggtg cacctttgac
    16561 gtaaatcacc gctccttttt cctccgcatg catcgttgcc atatatttgt attcagaact
    16621 gaacggaatc tttgcttcta tttttttcaa aggtagattg gctgtttctg tgtgtcgaat
    16681 cagcgcaact tctgtcggat tgccacttaa attttctgtt ttctgttctt cttttgtttt
    16741 taattcctga cagtttttca taatggacaa cacgcaggct tcttcttttg cagctacatc
    16801 cacaactgtc atttcatttt ttgtcaatgt tcctgtctta tctgaacaga tcactgtcat
    16861 agaacctaat gtttctaccg aaggaagtcc tttgatgatc gccttttctt ttgccatttc
    16921 atgtactcca acggatagaa tcatggtcaa aacagctggc aatccttcgg gtaccattga
    16981 caccaccaaa gcaatcgtag ccgaaagaag gatatgccaa tccataccat atcgaaatgt
    17041 cgtaaaaata actaagaata ggctcaaaca aagaatgcct tgaaaaattt gtttgtttaa
    17101 tcggttgatt tttttaatca gaggcgtcgt ttgttgttcg accgattgca atgcttgatt
    17161 gattttaccg atttctgtcg tgtttcctgt ttctacaacg acaccgactg cagaaccaga
    17221 ttgtacaagc gttcctgaaa aacccatatt tttctggtcg ccgatcgatt cttcttctgt
    17281 caaaacggca tgactttttt cagatgcttc tgattctccc gtcaaaatcg attcttccac
    17341 taataaatca tgtgcttcga ttaatcggat atctgctgga acgacatctc cggcacgtaa
    17401 gacaatgaca tctcctttta ccagttcttt cgcagggatt ttttcttgca tcccgtttga
    17461 tagtactatc gcttcttgtc ccatcagctg cttcaaacca ttcaatgatt cttccgcttt
    17521 tctttcttgc cagtaactaa cgagtccatt gacgatcact acgaataaaa taatacttcc
    17581 ttcaatatat tcactagaag caaatttcaa aaaagaggca atgatcaaca cgatcatcag
    17641 cagatcagta aaatgctttg ctattttttt ccatcgtttt gtctgctgcc ttgcttttat
    17701 ttcattatat ccttgatcag tcaggcgttt ctgtcgttct tcttgcgaca agccttctgt
    17761 tgatgtttga tagtttttca atacatcttc taccgtcaat ctgtaccagt tcatcctatc
    17821 ccctcctgtt taattgatac tgttattata cgcagggaag ttttttcagg cgtatcgatt
    17881 atttcagggg ggtctgaaat aattcgtttt gcaaaatgta tctgggccta actgatattt
    17941 ctattgctta ttcttttata tacaaaattt aagaatacaa accagataac caataaaaaa
    18001 ggaataaagc gtgtcgtgct ttcgaataac aagctgaaag caattgaagt aaaaaacacc
    18061 aacaaagcaa gatctttata tctttgtgta gtgttccgtt tcttttcttt aataaattga
    18121 acatgtgcac acaagatcga agaccacaca aatagaaaac aactcgttgt catgcctgcc
    18181 aaaagctgaa aaaacaatcg actatcagct acaaaaaaat gtagacaaag accggcaaaa
    18241 aataacattc ctgtaattaa aataccgtgt aaaggtacgc ctttcttaga aagcgcacaa
    18301 aaccaatggg gcaaactttt ttcttttgct gtttgatata aaatcctgct agtactgtag
    18361 actgcactat ttgctgagga caaagaagca accaaaatca ttagattgac aattgctgct
    18421 ccgccttggc aatggatcac ttgcagcata gtcactaaag gactttcttt taaatctaaa
    18481 caatgccatg gaacaacaaa taaaatcgac aaaatcgttc ctatatagaa aaagagaata
    18541 cgccaaggca gacagttgat tgcttttttc aaattctttt gcggatcact tgtctcgcca
    18601 acggttaagc cgatcatttc aattccagca aaactgaata aaaccatagg aaatgagact
    18661 accattccag caaagccaaa cggtagtaat tcatcgatct gtccaaaatt ttctttgatt
    18721 ccgatagact gggtatgaaa atctgagaca ctcaatccta tacccaataa gataaaaagg
    18781 aaaatcccca agatcttgat gattgagaaa aaagattcca attcaccaaa ccacttgact
    18841 gcaacaaggt tgactgccat taagatcatt agtgcgctac ctccgcttat caaagctggt
    18901 acatgtacaa accagaaatg gatatacaat cctattgctg ttaattccgc aagtccagct
    18961 ccaagccagc aaagccaata agtccaattg acaaaaaatg cccacttttt gcctaaataa
    19021 tagtcaacga aatcactgat agagttaaat gtactatttg ctaaaaataa atcacccatg
    19081 gcccgcatca aaccataaat agccaaaccg catatcaaat aaacgagaat aacagctggc
    19141 cctgctgatg caattgcctt tgctgaacct aaaaataatc ctgttccaat tgcgccacct
    19201 aatgcgatta actggatatt tcgattagtt agagtccgtt tcattgccgg tttttccatc
    19261 aagttcacct ttttcttttt caagcagcat ggcgacttcc tgttcccaag aaggtttctt
    19321 tattttttca atacagagtg gtactacaaa caaaatgaca ctgatggctg tgactgcaat
    19381 tagattgatc catgttccag ctttcatcgt cagtccgaca gaaaatccga ttgccgcaaa
    19441 taaaacaata acgacagtcc aaactccaag attgctcttt ttctcttttt ttccaattct
    19501 aaatggtcga ttcaactttg catgtgtata acgcaatctc aaaactgcga tccccatgat
    19561 gacatagacg atacagtaga taacagtagt tgaatttacg agcatcaaaa atgcttgatt
    19621 gacgccagga atcagcaaat aaaaaagtgc aaagattgag ataatgacag actgagtcaa
    19681 aataacggat ttggatacgt caaattgatt cgtcttccaa aaattgaatt ttggtggata
    19741 cgcacctttt ctagcacttt ctgtcactgt ctttgctggt cctgaggccc aagcagataa
    19801 ttggactgct acaccaatga agacaagtaa actaaacaga ttcacaataa gatgtggcaa
    19861 accaagaatt tggcaataaa tcgaaataga ttgcgcgata ttattcaatt ccatttgtcc
    19921 ttttggtacg acatttgcta caactaaagc attcaaaata ttgacgaaaa atataaaaat
    19981 taacgcggca aatactccgc gaatatacgt tttaactgga ttttccaatc gagtgatata
    20041 cacagaggac atttcgatac cagtaaaaat aaacatgatc ggcgcaaaat aaacaaatga
    20101 ggatgcagtc gtagtatctg gtattaattt gtcccatgaa aaactgccta atgttccggt
    20161 tggtacgatc ccaactttga tccacgcagc aaaccctaat aagagcatca tcgtcaaagg
    20221 cacgtacaat cctaaccaaa caccgacttt tccgccgatt tttgccatat caaatttcaa
    20281 attcaaaaaa gtgatgatcc aataaacgac taaaatgaca actaatgtaa atttgctgtt
    20341 caaaccaagc ggcacattat cgataatatt accaaacaac ggagcgagtg ctgaagcgac
    20401 catcaccatc ccaggaaaca tttggaccca taacagccat gagacaacga acccccattt
    20461 ttttcctaga gcattcgata cccaaagttc tggaccacct ttttggggaa acattccagc
    20521 aaattcacca gagatcaatg agattggaaa agcgtacaat aatactgcaa ccagcatgta
    20581 aaaaatcatc gtccagctcg ttgctgctac atctggaata tttcgtacac ttgctacaag
    20641 ggcgcaggtc attccgataa aggtcatggt cgtgatttgc tttgatgaac cagtatcttt
    20701 catctttatt ccttctttca atagcgacat atgtttttgt tttcttttat ttttgttcaa
    20761 gaacagaaat gacaaggttt catcgattca gtacaaaacc cagtatcacc gaaacatcca
    20821 ttgaacgatc ttagtgaact gggtttgata catgaaacga acgatccaac ttatagaacg
    20881 ttttataaaa aagaatatct tggcgaggaa tatatgaaaa acagggggaa atacaattgt
    20941 tttgatgaag caaataattg tctgttctcc atatgtctct tgttattctt tcacacaaaa
    21001 atcagttcta ttggatgatt tcgtttagtt tcttttccat tgctttggcg attttttttg
    21061 tataaaaatt gaaaattcga tcatcattca aataaggagt catgatagcc gcacgtagca
    21121 gagtgatttt ctgttctttt ttccattctt ctttcgttag acccattctt tcaacaaaac
    21181 gaatcggaga atcaccgtaa tcttcttgtg taaatgtagt atgagaagta ataaaccgtt
    21241 caccataaac atcgccatcc atataagaag accgatcgaa cattttttca tttaattcat
    21301 tgatagcgtt caaatctgta cagccttgtt ctttgaatac ccaattgacc atattaaaat
    21361 caggatgatt caatggatag acttcgattg tttttccttt aacagtaaaa gtcagatgat
    21421 cgagaaattc tctgaatctt tgagctgcct cgattgaagc cccaatcaat tgaccatatc
    21481 cggtcacatt caatggcaat acacgatgtg ctgtccagac tgcggcggcg gttgctccag
    21541 cttttgatcc ttccaaaata taagctccta acatatctgg agcctttact gatttttcaa
    21601 acacatatgg cgcgaaataa gaaatgatat ttctcatatt tttatgtttg attacgattc
    21661 ctccagcagc ataagggaca tagcccatct tatgaggatc tatcgtgaca gaatccgctt
    21721 ctgaaatagc tctgaaccct tcatatacct ctttgtcaat cgttacgcag tggtggaaaa
    21781 catgatgttc ttcaaagaat tcagctaagg aagcatacgg cacaaattct cctgcttcgt
    21841 ttagaaacag tgaacgcgca tagccgccat atgcggcatc aacatgtaaa tagaaataga
    21901 tcccttcatc ttttaacctt tctcttaatt ggacgatttt atctacactg tctacttgac
    21961 cttcttctgt cgttcctacg acagcaacca cacctagaat tgggattttt tgtccagcta
    22021 attcacgaat tgttttttcc aaagcattaa tatccattcg ataatcctct tgaactggga
    22081 tagcaaccat ctgatctaag ccgactccgc aaatatctaa cgccttcatc caagaatagt
    22141 gttttgtttg agggaccagc catttcccta atctttggat attttttccg ctacgggaag
    22201 aagctgcttt gacctcatcc atttcttcgt ccgtaaagct ttcggtcatt tccaaaattt
    22261 cttcaacaga taaattcaaa agttcccact cagacatttt tttcactttt tcaggcagca
    22321 cttccttgac agctaaagga atcgatttga tgcaacgggc gtaccataat ccttcaagat
    22381 tagcaataga accatctgct gcaatgtgtc cccatccgtc tgtcatattg aataatgaag
    22441 caaaatcctg tcctacttct gcttccattt gcgaagttgc catagaagat tccaatgcaa
    22501 cattatttgg gttccacagc atcgcatagt tgtaagctaa taaagcaggc atcaatgttt
    22561 cagcattcat ttgtccccag taacggcctg ctgaatgcca tgggatcgat cctgcgcgta
    22621 atcgctgact gacttcatcc aagacctcta tcatatggtc gcgggtggct agataatctg
    22681 gagtgagctt atctccttcg ctaattgcag gcatatctga aggaatataa ttttctctcc
    22741 atccaagatg ttcatctacc atcttgttca acaacatttt ataaactggt ccattttcag
    22801 ctttgtcgcc tataaagacg gccttgatat ccatatcttt catttttctt ccctccatat
    22861 tttgttggtc aatgtcttgc agatacatgt gtttatctgt aacacctctt cactatagcg
    22921 ccaaaaatga gaattttttt tttataacat caaatccacg tagattttta caaacaaaaa
    22981 taaataaagt cggatttttc tgataattca acgcaaaaaa gcgaacaatt tttcaataaa
    23041 tcagtcttct aaaatagata acctaaaaaa agcgaacaaa caaataaaat gactgtcctt
    23101 cattcactaa acatattttt atttttttat attgttaaaa attcgcaatt tattttttaa
    23161 tagaattatc tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat
    23221 gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat
    23281 tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata
    23341 ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa
    23401 agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa
    23461 aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat
    23521 atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt
    23581 ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg
    23641 aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt
    23701 ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac
    23761 gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg
    23821 aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat
    23881 tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc
    23941 agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta
    24001 tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag
    24061 aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc
    24121 agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa
    24181 taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac
    24241 cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca
    24301 tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga
    24361 caattttttc cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca
    24421 tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta
    24481 tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg
    24541 taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga
    24601 agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg
    24661 cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt
    24721 cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt
    24781 gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt
    24841 ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac
    24901 taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat
    24961 cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat
    25021 agcattgttc tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa
    25081 aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag
    25141 tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct
    25201 acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg
    25261 actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata
    25321 actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct
    25381 agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga
    25441 gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa
    25501 caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata
    25561 tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt
    25621 gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact
    25681 ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt
    25741 aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg
    25801 tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc
    25861 tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt
    25921 ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa
    25981 acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc
    26041 ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt
    26101 tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt
    26161 ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat
    26221 tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat
    26281 caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt
    26341 ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg
    26401 cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc
    26461 ttctgttact gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca
    26521 ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa
    26581 actgggattc aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc
    26641 agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt
    26701 ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc
    26761 tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca
    26821 tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat
    26881 cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga
    26941 attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac
    27001 actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa
    27061 tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat
    27121 ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg
    27181 aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac
    27241 gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa
    27301 tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa
    27361 tatgtctatt gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt
    27421 tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat
    27481 taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc
    27541 tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat
    27601 ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc
    27661 aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc
    27721 ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca
    27781 ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc
    27841 tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc
    27901 cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt
    27961 ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagc
    28021 tgccctgcat caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg
    28081 gttagactta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa
    28141 aggcgtttag tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca
    28201 taaatttgac ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt
    28261 ctgtttcttt ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca
    28321 tctaacggat acaactattt caatgattac cattacacta aaaaatcgga aacacacctc
    28381 tccgaacaca aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta
    28441 tcaaaaaggt aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat
    28501 tttatgaatc tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa
    28561 atggaaatca gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt
    28621 atcacagcaa cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt
    28681 ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct
    28741 caagttcaaa cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt
    28801 cattgtcatt tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag
    28861 ttatatggcg ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg
    28921 gaatatctaa gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt
    28981 cctgatgaaa aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa
    29041 aacgcaggag taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta
    29101 ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta
    29161 cagcaccgtc tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac
    29221 acggcacaag ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc
    29281 gattgtgtag gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc
    29341 ttacaaagcg gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga
    29401 acagtccaag taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt
    29461 tttatgcggg atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa
    29521 atttacgcaa tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg
    29581 ccactatcag aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata
    29641 aaagttgttt tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa
    29701 atcaaacgaa atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga
    29761 cgtcgcttcc gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta
    29821 ttaaccggga ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga
    29881 tatggcacct atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga
    29941 atcgtaagga acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa
    30001 aaagaggcat gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt
    30061 gatacagacg agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt
    30121 atcgttcata aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat
    30181 gcatttcttt tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg
    30241 gttcaacaaa cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt
    30301 caatgacgcc cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag
    30361 gaatactttc cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa
    30421 ctccccattt tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta
    30481 atttgatcca gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa
    30541 aaattataca agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa
    30601 cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct
    30661 tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa
    30721 attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat
    30781 tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc
    30841 aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg
    30901 gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc
    30961 aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc
    31021 taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg
    31081 tttgttcacg ataaacggtg cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat
    31141 ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa
    31201 gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag
    31261 tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa
    31321 tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa
    31381 tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt
    31441 ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat
    31501 aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga
    31561 aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa
    31621 gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc
    31681 tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa
    31741 gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga
    31801 gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg
    31861 tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt
    31921 acgcgttcaa tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag
    31981 atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt
    32041 gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg
    32101 catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt
    32161 ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat
    32221 ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt
    32281 tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca
    32341 tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact
    32401 tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa
    32461 gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt
    32521 gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc
    32581 attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt
    32641 taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac
    32701 actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta
    32761 acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat
    32821 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt
    32881 tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg
    32941 tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt
    33001 tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc
    33061 gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt
    33121 caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa
    33181 tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc
    33241 gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta
    33301 atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc
    33361 gttttctgtt atcagccaag ggatattatg ataattttct tttagataca tggctatatc
    33421 atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa
    33481 atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg
    33541 aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg
    33601 ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa
    33661 ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata
    33721 aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac
    33781 catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc
    33841 tactgcttta gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt
    33901 ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc
    33961 gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata
    34021 cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa
    34081 agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga
    34141 gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc
    34201 agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat
    34261 cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc
    34321 attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc
    34381 tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt
    34441 ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac
    34501 gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt
    34561 gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca
    34621 ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac
    34681 caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca
    34741 tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata
    34801 acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac
    34861 cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa
    34921 tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa
    34981 gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga
    35041 cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg
    35101 ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga
    35161 taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac
    35221 tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc
    35281 tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt
    35341 tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga ttttcattca
    35401 ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga
    35461 atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga
    35521 tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta
    35581 aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa
    35641 acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta
    35701 tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta
    35761 atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc
    35821 tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt
    35881 tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa
    35941 ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat
    36001 atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat
    36061 ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa
    36121 caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc
    36181 ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg
    36241 aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc
    36301 atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc
    36361 tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc
    36421 tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc
    36481 tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc
    36541 atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat
    36601 cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc
    36661 atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta
    36721 tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa
    36781 gactaggata aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat
    36841 gatgttcaat aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat
    36901 atatggataa aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc
    36961 ttcttctggt gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa
    37021 ccgacttgct ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc
    37081 catttgcgct aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc
    37141 cagagttgat ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga
    37201 atcttcagga ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa
    37261 cgtgattgcc cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc
    37321 acgagaaaac atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa
    37381 aatttcagtc attttttttg tatcttctcc attatagttg gatataccaa tatataatgc
    37441 ttttccttgt cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg
    37501 gtctgggcga tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg
    37561 gtcacaactt gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat
    37621 atgataacct gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct
    37681 aaaaatacgc ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag
    37741 gtcgaaatgg gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat
    37801 aggatcataa tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc
    37861 tgaatgaccg gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa
    37921 tttttctcct cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact
    37981 acattttaaa gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt
    38041 tttttactcg ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt
    38101 tcggttgttt tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg
    38161 gttcgatttt tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg
    38221 attgttccgc taattctttt tgtcgttcga cttgataaaa aatcactgga cgatattgct
    38281 tccctctatc ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg
    38341 tcagcaactc gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat
    38401 gacctgtgta accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt
    38461 atcctgacaa aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga
    38521 aacatccccc tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg
    38581 aaaagtcata tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta
    38641 catcaggcgt cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata
    38701 actgttcttt aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc
    38761 gttcccaatc tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt
    38821 gttcctcatc catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta
    38881 atttatcata aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa
    38941 aaaagagaga atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa
    39001 agttaattta ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg
    39061 taacaagata agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa
    39121 ctcctttgac tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt
    39181 gatgatttat ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc
    39241 tcttctcatt agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta
    39301 cccattgact gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag
    39361 cgataggaga ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg
    39421 tttctttgtt tacaacaata tgtcccggct gagaaattat tttttgattg aaattttttt
    39481 tactagaaac agttaaagtg attaataaca aaacagtcat aaataaataa aatagttctc
    39541 ttgtcttatt gtatcttcta tttttgaaaa atatcatcgt taatatcaga gaagagaata
    39601 ttatttgcaa gcttaagatc cgccaaggaa attgtaacaa tttggctggc gtattttgca
    39661 gaagatacca tggcaccaaa ctagttgtag ctagccaagt caaacacgta ccataataaa
    39721 tctttttttc tatgtttgtt aattttttaa ataaaatcag gggaatacat aaagataaca
    39781 gaccaactag tcccattgag taagtagtta agtcgttgtt catagcttca gtcagactat
    39841 cgaacacatt agatgcatga gcatataaat ttgtcacgct aggtttgttg attttttggt
    39901 agagcatttg ttcaaacatc ggataccaaa aagcactcgt cagaaccaat gtcattcctg
    39961 ccgcactaaa taatttttta aatatagcaa taaaatctgc attcttgaaa cctttagaaa
    40021 ccaaacgaat aaaaataaaa atgataatta atgctgtact cattaatgtt gatagtacat
    40081 ggctgtacac tagcaaactc atactcaatg ctagctttac agacggatac ttttcttctt
    40141 ttccagagat gatttgataa gcataaaata atacagggat aacaagtgtt attgcgatag
    40201 cttcggcaat tgcggaacga tagtagatgt ctactgtccg ataagcagag aaaatataga
    40261 gacaactaaa taaaaatgcc gctttttttt gttttaaaaa ccgctcgcca taatgataag
    40321 ctacgagtgt tgtacacaag ttcaataacc aaacgtaaat aatatagctt acaatcaaat
    40381 tttttgtcat ccaatataac attacagctg gaaaaaaagt cagaaaggga taaaaataat
    40441 ttacaccata tccagtataa ttgaatgtct cataattaat cggactggta aaaatagtcg
    40501 ataacccctt gattcgagat aagtgaaaag aaagatctcc agtggtataa atactctgtg
    40561 tatttttgaa aaagatgaaa gaatatatac tactaactat aattaatcca ctcaatatta
    40621 ctactttttt atttattttc attcttcttg tttatctcct ttttagtcac atatcgagaa
    40681 tctttcaact tattaggtgc aaatcgttta aaacttgaat acaccactaa acaataagtc
    40741 agaatactga tccataaagc catgagagca gttggagtat attttgttgt aatttctatc
    40801 ttattttctc cagcatttac atccacattc actaagccat gttttttata agagtcattt
    40861 acgactttac cgtttacttt tacctgtgta cctaaataag catatactgg tactgtcact
    40921 ttttgctttt tgttgcttgc cactgtgtaa gtaatgttat tatctgcagc tttatacttc
    40981 cctttttccc acgattctcc tacttttact tcatgttttt ctaagtgagg cctttctttt
    41041 atcgcttctg ttggggcgta atcgtataat cctcccatat ctgatgttgt ataacgtggg
    41101 agtgtttctt cattgataac aatatgaccg tgttttgatt ggataacttt tgcgtaattt
    41161 tcttttgctg ctatagacgt gacgagtaga aacaccacag tagcgccaat gattaaccat
    41221 gaatattttt tattcatagt ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa
    41281 aagatttgga tacctaagat acgccaagga aactggagta agcttatcgg tgttttttga
    41341 aacaaaaacc atggaaataa attagttacg catacccatg tagaaactac gccaacatag
    41401 atgatttttt cttttttctc catttctttc agtatgaata aaggaaaaat caacgcaacc
    41461 aatcctaata accccatagt gtatgttgta atatcgttat ttaacgcttg aatgatacta
    41521 tctgaaatag aaagtgcttc tagctgcaaa tctgttttga agggactgtg tatttcttga
    41581 taattcatct gctgcaacat aggataccaa aaatagctag ttaatgcaca agtcaaaaaa
    41641 acagccttta ctaaattcac gaccacaaat tttatatcgt tttttatttg cttgcttaaa
    41701 aacaaacgaa ctataatcat acaagcaatc aatgcactac acaatagtgt agttaagaca
    41761 tgagtataca caagtagact tatactgaca gataaagcga tgactttttt ttctctattg
    41821 aatatcaatc cataagcata atataaaaca gctgggataa tcgtaagagc gattgcttct
    41881 gcaattgcgg atcgataata gatatcaact gttcgataag ttgcaaaagt atataaacaa
    41941 ctaaataaaa aagcagcatc tatcttttta aaaaactttt ctccataata aaaactaatc
    42001 agaatcgtac aaatatttaa taaccaaata tatagaacat aacttaatat taagttattt
    42061 gaaattccat aaaaaacgac tgcaggaatt attgtaagaa aaggataaaa atagttcagt
    42121 ccatctccat aattgttaaa tgttgtatag ttgattggcc cttcaaaaat agaagataac
    42181 cctttgattc gtgaaagatg aaaagataaa tcacctgttg tataaatttc ttgagcattt
    42241 ctcataaaat ttactgaata taaagtacta agtaagataa gaactactaa aagaaatgct
    42301 tttttactta ttttcataga tttgttgttc tcctttttta attctatttt cagtatacta
    42361 gaattgtctt tttttaataa gttttgcgtt aaatttttca tagcactagt atgatatact
    42421 agaaaagaat caatttttat tacttttaaa taggagaaaa aaatgaaaaa aaacaaaaac
    42481 caaataatag atatcatttt tatgttattt ctaagttttg cttacgtcat ccctctattc
    42541 atgtctaagg gaatctatca tagtgtcaat caggatactt actttcattt atctcgaatc
    42601 ataggactag ataatgtatg gagttctcca gtaaacttca ataacttcga tcatcatgga
    42661 acaatgatga atatttttta tccatggctt actctttatc cagctttttt attttataaa
    42721 atgatgggaa atcttgtttt aagttataat atctactatt tttttatcac ttttttaaca
    42781 atggtcgttt cttatttttc tatgaaacaa ataaaaaaca atcgttatat ttcgctgtta
    42841 ttttcgatta tttatacatt ttccgcctat cgtgccattg acatattcca tcgtgcttca
    42901 ttgggagaag ctgtcgcttt aacatttttg cctttgatac taatggggtg ttacgaaatt
    42961 tatattagag attaccaaaa atggtactgg ttgtctattg gcatgacgct agtagtgtat
    43021 actcatttac tttctgtcgc tatggtttct gtatttattg gaggaacgtt gtttctttct
    43081 ttttattttt gggaccaaaa aatcgctaga ctcttaagtc ttttaaaagc taccgtcctc
    43141 accttttttt taagtgcggg attcttaatt ccttttattc aacaatctag agcacaagaa
    43201 ttgaaagtcc cactgggaaa agaattaagt ggtatggctc ctagtgatat gctgacacat
    43261 attttaaata ataactataa caactatact attggattat tcttatttct cggattgatt
    43321 ggtgctttta tttttataaa aaaattaact gctgatgatt tatttatttt ctttcttggt
    43381 gtatttgttc ttttttgttc aaccaattta tttccttggc aactttttaa ccatacacca
    43441 gttaagagtc tacaatttgt ctggcggtta aatggttttt cttctttatt tatagcctat
    43501 acgatgagta tagtgatcta ttatattttt tctactaaaa ataattcatg gaaaataatg
    43561 gtctttacgt ttcttgcact aatattgcat ctctcaggag taagcaattc tatccatgct
    43621 aacaaagatt cattaacact tatagcacct gaagacgctg ttgctatagc tgaaaattac
    43681 aatcatactg attatgcaaa caaagagtca atctatcatc ctgatatgat taataatgat
    43741 caatatttac taggtaacca ggaaataaat ccaacaactc attttatgag taacaaactt
    43801 acaattgaat tagataattc gaataataaa aatactgtct tgacgacacc tatttatcgt
    43861 tataaagggc aagtggcttc tattaatggc aaactagtac aaacaaaatt atctaagttt
    43921 ggcacaactg aattaactat tcctccaggt ataaataaag tcgttattac ttatcagtac
    43981 acaaaactag caattgcttc aagatattta tctatcgtta ctttgatttt atttttactt
    44041 tatcgattta ctttttctaa acaaaaacaa ccgagaagag aagtacaaca ttctcactag
    44101 atttttattt aagaaagact ttttagctgt acacataaac aggctaaaaa gtcttttttt
    44161 atatttcttt aatttgcttc atacattatt taacatcgat atttatactg tgaaacatgc
    44221 gtttcatcca ttatcaaagt atttttatcg ataataaact tcatccaaaa acagaccttg
    44281 tgcagggacg gtttctcctg catgttgccg cactttgctt tcaaatattt catcgattgt
    44341 ttctatcggc atggcacctg tgccgatttc tagtaatgtc cccatgataa tccgcaccat
    44401 tttatataag aatccgtctc cgacaaaagt aaaatgaagc atttctccct ttcgttcgat
    44461 agataattca tcaatcgtac gcactgtgga tttttttgat tttttcaatg cagaaaaacc
    44521 aatgaaatca tgtgtaccca ctaatttttc gcaagctgca ttcagcttgt ccatatccaa
    44581 ctcttgaggg aaataaaagc tgtggtttcg ctcaaatgct gaaggaatca cgttgttcca
    44641 aacataatag ctgtattttt tgcccaccgc attatatctt gaatggaagc gctcaggcat
    44701 ttcttcgatt cttttgatga caatatcccg cggaaggtaa cgattcataa agtcgatcat
    44761 ttctgccagt ccaatttctg tgtttgtttt aaaatttgct acttgtcctc gcgcatgggt
    44821 gccagcatct gttcttcctg atccaataat ttctatctct tcatttgtca tctgatgcaa
    44881 tactttttcg atttttcctt gaatggtttt atcgtcatct ccaagtcttt gccatcctga
    44941 atatcttttt ccgtcatatt cgattgtcaa cttgatgttt ctcatatttt agtggctcct
    45001 ctgtcttcca tttggtcttc tctctacctt tttaagattg tagcacaaaa tatagctcct
    45061 tgcttctcta agacagaagc tatacaagaa aactgtggaa atatatattt cagaaaaaca
    45121 aaaaacagcc accggatgaa gtatccgctg actgttcaat ttgacggctc aagttttact
    45181 gtttgagatg caagatactc aaccaggcaa tgggcacata attaaagaaa aaactgattc
    45241 tttggcaaaa acctatttta ttcagtaatg gccaagaatt tatagaagga attcttgcca
    45301 gtccataaat cgccgcaaaa agcaaactga tcacagacag tccaaggtat aagtggctta
    45361 cgtccatttg cccatttttt cgatagatca agaaaagaag aactggaaac aataaaaaac
    45421 cactgtaacc taaacctgat ccgatattat ggacccatgt cgagacattc cacgaagatt
    45481 catgggtatt gatactgaac aaacctgtaa agatacaatc acctatacca tagaacgcga
    45541 tggctgctgt aactagggta gctaacacac gagatgtact aacaaatgca gcatacaatg
    45601 caggcaatga caggatgaaa aagatccctg aaaccacaga ccaaataaga aacgcttttc
    45661 ttacaggact ggaaacctct ccaaatacac tgatcacgct cttcatttga tctaattcag
    45721 gataatacaa tcctaatacg tacggagtca aaaaatcact cagcacgcct aataataaaa
    45781 agaaaaaacc aaatttacgt aaaaaatcca tgatacgcct ccaataacaa ttacctatgc
    45841 ttttaattgt tattataaac gactttatct tgatttaaca gaaacctcta tttacacgaa
    45901 catctatata actcattaaa aactcgaatc attttaaaaa tactaaatat ctaacgttca
    45961 acgttcactt aatccatcta aacaatcttt caatttgatt ttttccccac ttcttttacg
    46021 cgcgcctagc tctcctattg cagtaaacaa cagatcactt gacgaattta aggcagtttc
    46081 cattgaatcc tggataacac cgataataaa tccaataccg acgatttgca tcgcgacatc
    46141 attagagata ccaaacaaac tacatgctaa tggaatcaat aacagtgaac cgcccgcaac
    46201 acctgaagct ccacaagcag ctaaagcaga caaaatacat aaaaggactt tcaaaacaaa
    46261 aggtgcttca atccctaaag aatgtacagc agtcaacgtc atcaaggaaa tggtaatcgc
    46321 ggcaccgccc atattgatcg tagcacctaa cgggatcgat atggaataag actcttttgt
    46381 caatttcaat ctttcagcca acatcatgtt tatcgggata tttgcggcag aacttcgggt
    46441 aaagaaagcg ggaagtgcgc tttctttcaa acaaaagaaa atcaatggat agggattttc
    46501 tttaataaac aaaaacgtga ggaatgggta cacaactaat gcgacaaaca gcatcgtacc
    46561 gatcaaaaca agtaaaagct gcagatactc tgccaatcca gcgattccag tcgtagcaat
    46621 tgattgataa accaatccta aaatccctat cggagcaaat gcaataatca cttgtacgac
    46681 ttgtgacaac gctatagata tctgatccac caactctttt gtcgattctg aacgtgctcg
    46741 aaaagctagt cccaacaata cagcccagaa taagacgcca aggtaattcg catcaataag
    46801 agattgaaca ggattttgtg tgacattcgt caacatagta gataaaacac tttccaaact
    46861 ttgcggtgct ttttcagata ctgtttcttg taaagtaata ggaacagtaa aaattgaact
    46921 tacaataact gctacaagtg cagaaagcaa tgttcccact aaatataaga tcaaaatagg
    46981 cttcacgaat gtcttattcc caattttatg cttggcaatt gacgccatcg tcaagaaaaa
    47041 aaccagaata ggtgcgattg ccttcaagca actgacaaac aacgttccca atattgaaat
    47101 aaatgtccat gtggggaaaa agataccaaa caatattccg actaaaatac caacaacaat
    47161 acgttgaact aacgacatat ccaaaaactt ctctaacaaa tgaccaccct ttcacaattt
    47221 taacaatcat tgtatcattt tatgcaaaga aactaaaggt ataattttct aaaaattcta
    47281 acaatacaaa caaccatttc ttgctaattt tgtacttttt ctgtctacac gttgtttttt
    47341 gagatattct aagttaggat gttattaatg ggcaacttat caggaaacgt tttcttatgt
    47401 ttttgtccaa tttttttgtg aggtgtttat tcttgaaaaa aataaagaag gagagttatg
    47461 gcttcctgac aatctttgtc gcttgtataa tgatggtttt acagcgtata ttgatttcag
    47521 cagtcactat tcctacatat gcaacaaacc ctggtcgatt ctacatctat acatttttac
    47581 aaacttccgt tgttattggt gcaaatttga tcccacttta catgggttac caatatcaaa
    47641 aaatcgcccg tttaaaagtt ttacattatt taagtcgttt tgcctttata ttcctaatag
    47701 ccacactagt ttgcaatatc tttttttacg tacaagctgg cagcttgaat attcgtgatt
    47761 attggcttat cctaacacct atcagtcaga attactttac ttatgctgtt tcttgtactt
    47821 tgcttttttg tagtattcct tatacagttg gatttttaaa taaattatcg aaaacaacaa
    47881 ttaaatatct actattattt ttaacaattg gattagttgg ctgctctact ctatttaata
    47941 aagacccgtt tgcattacag aatggacaga gtattttatg gattttttat ttatttttaa
    48001 tgggttatgg tttgaaagaa tggaactgga aagaaaaggt ccgctataag cttcctcaag
    48061 tatttatcag cttgattgtt ttattcggtt tgattatttt gatgacacaa atttcactca
    48121 tcatcagagg aaatacagat acagcgctga ggttttctaa accatattct ctcttcgcaa
    48181 tgtattatac tttctcttcg tttttatttt tagaaatgct atctgataaa ttaggtataa
    48241 aaggaagagg aaattttctt gctgtatttt tgattgtcac tcaagttata tgtaatttcc
    48301 ctttagtttc ttatcgcgta tctacattta tgaaacgtga atttccaaac tcagggaagg
    48361 catggctgat aaatatcggg cagtttttta tttgatattt attagcagtg tccctcctag
    48421 ttatcttttt attttatata caaaaaatcc gtttcattca tcgtttcatc gaatacttcg
    48481 cttttgattc gattgaacaa ttaactcagc gttggaaaaa aagaattcat tggctttttg
    48541 ttcataaaag ttatttttta gttgcagcat ttttttactg ctttaccttc gtgcagattt
    48601 tcgttttaga accaaaagaa ggttggaaag aaactatcca ggttgcctta aaaatattta
    48661 ctcagcgaca agcacccatg atcctgacaa tcatcattat tatgggattt ttcttcttac
    48721 tattgttgat ttccaatcgt ttttggtacg ccttgactgc taccttgatc attaacttac
    48781 tgctcactat ttcgacagta atcaaaatgg aaatgcggga agagcctgtc tttccttcag
    48841 atttaaaaat gctgaccgga ttgtcagaac tattatcgat ggttagccca gtcttattaa
    48901 tcgtgggcgg attgatcctt ctcctattat tgattactag tattattgta caacgtagac
    48961 tacaaaaaca gtattccttg aagattcatt ggaaacgtcg atttatcagt atcgctgtat
    49021 tattaggatg tttttcgggt gtctttttca ttaatcacaa aaattcgcct tctttcttgc
    49081 tgtttaattt atttaaagta aacaagactt tctttaacca gcaaggcgcc gtaaaagata
    49141 acggaccagt cattcaattc ctaaacaata tcgacattaa aatcatggaa gaacctgctg
    49201 gctattcgaa agaaaaaatt aatggaataa tgaaaaagta caacacaaag gcagatgaaa
    49261 tcaaccaaac aagaagtgac tggttaaaca atcagacgat cgttctcaat ttaagtgaaa
    49321 gcttcagtga tcctgcacgt gtccctaatc tgacagtacc tacagaccct attccttaca
    49381 tcaaaagcat tataacagaa ggaacaggag gattgatgtt atccgtcggt tatggtggcg
    49441 gaacagctaa catggaatgg gaaggactga caggcttgag tatcagtaac ctgtctgctt
    49501 cgttagttac accttatacc cagctagtgg agcgtcagaa aataagtcca aatattaccg
    49561 atctattcga tgaaaaaata gccatccatc cttttacagc tgctctttat cgcagaaaac
    49621 aagtgtttga taaatttggt tttgacaggt tctattattt agacagtccg gataaattaa
    49681 cttatacgga taaaatagaa caaagcccac gcatatcgga tgattcagca tacaaagaaa
    49741 ccttgaaagc tttacaaagc aatacggaaa cgtcacaatt catccaatta tccactatgc
    49801 aaaaccatat gccttatgat gattactata gtgaattgaa ctacactgct gaaggaactg
    49861 ctgtcattcc taatagaaga aaagaattac aaacttatat gcaaggcctt cactataccg
    49921 atacagcagt aaaaaaattc atccaagaaa tcgacaaaat cgataaaccg attacctttg
    49981 ttttttatgg tgaccattta cccgcaatct atagtgggaa tgattcgaaa aaatatggat
    50041 tagaacagca ccagacagat tatttcattt ataacaacaa gtatagtcgt gaacaagcag
    50101 ctacgttaaa taaagaagtt gttgctccta ataactttgg tgctatggct cttgagcatg
    50161 cgaatatcaa agtaactcct tactatgcgt tgttgacaga agtatctaat catctacctg
    50221 cattaacaat tgatcctaca gaaagcttgt ctaatcgttt taatggaaaa caggtctttg
    50281 ttacagagga tagtcaaatt atccaagaaa aaaatttaac tgagaaacaa aaagaaattc
    50341 tagaagatta tcgtctgata caatatgatc ttgttgcagg taaccagtac agtgcaacat
    50401 gggcgacaca aaaaataaaa gactaacaaa taaactgagc aaggggttgt gataaaagac
    50461 atgtcacaac cccttgttta tctcattaat ttgcttgtcc ataaacgatt tgattaaagc
    50521 catttttcaa ttcattattc gctgtctcgt tgaagtaaag ataattatcg ggattcccct
    50581 gattttgttg tgtcaccaat acttttggct gattttgctg aaacccgaat agatagacgt
    50641 gctttttcag atatggttct gtctctgcat cggaatagac ggctgtgatt tgatagtctg
    50701 cttgacctgt cccactttct gaccattctg cagaaacagg cactccacca atagccatct
    50761 tccactcccc gttcaaaata gcttgaggga ctttaagacc atataagtcc acactcattt
    50821 gattcgtata acttttgtat tgttgtccca ttgtttggcc ccatttggtc ataaatgcct
    50881 ctaattgtgc ggatttgtct gcattccaca gtgtttgttc aacttgttcg ctgctttctg
    50941 aagaggaaga tgaagcctct acagaagaac ttgaagtaga tgtttgagtt gtttcagtcg
    51001 ttttggaaga ctctgacgaa gtagaggtat ctgctgtttc tgtacttgct gcagttgact
    51061 tgctctctgt gctagttgtt gtagtagttg caactgttgc tttcgctgtt tcttttgttg
    51121 attctttttt cgattcattc ccgttggtac aaccagctaa caaaaaagct agaccaattg
    51181 ttactaaaat cctttttttc aattaaaaaa ctcctttcga ttactcccac ttctttattc
    51241 tatcaaataa gcaatgtgat tttattgatt tttcgtattt tctcatcaat ctttcaaatc
    51301 ttctaaaaac tgagaagcac ccgctgatgt tgtctgtaat acatcagcgg gtgcttcttt
    51361 tattttaatc ttcgtgattt tccaatttac ctcacgcttt atatttgtat ttgaagaaac
    51421 atagactttt agtcatcatc gtcttcctca tccaaatctt gtcggacagt ttttccttgg
    51481 agtctttgta cttggtagat gatttcttta ttgaaactta aaagttcaac aagcaattgt
    51541 tgcaagccat atttttcttc tttttcagct aatttgattg cccgtgtgat aaagacattt
    51601 tggaaatcaa aatcttttgc aaagttttcc aacattgttg aggctggtaa atatttgttt
    51661 tgaccatgtt cgtaaatttt tccataacgc atgacttctt ctaaagtaga cggaacgatt
    51721 tcattttcaa tcactaactc atgagagatt ttattgaacc atctcgcttc ttgatcagca
    51781 tattctgaaa aaagggtact taattcttgc gaagatgtac ccaaaacata caattgtgct
    51841 tggatcaatt tcatttcttc taatttaaaa tttgctaata catgccccac catggcgcca
    51901 gcagttggtt tatgatggtc gatatctgct tgttttgttt cttgaacaaa tttttcttcc
    51961 actatattca ttgatttacg cctcttttac ttttgctgat tggacttcat aacctaattt
    52021 ttccacaact gcagctaatt gttcagggtg gacttcatct ggatcaaagt ttgctttgat
    52081 tttgctcgca ttaaacaaaa ctttcgtatt gcttacccca ggctgttgag aaaccgcatg
    52141 ttcgattttt tgcatacagc ttggacaagc tagtgtatct aattgcataa taattttttt
    52201 catcgtagtt cttccttcct ttttcttgat agtcttattc tagattacag cttccataaa
    52261 aaaattgatt cttatcaagt tttacgattt ttcttctttg ctttcgtgtc caaaatacaa
    52321 taaacgcatc gcgttaaaga taacgatcaa gatactagct tcgtgaacga acatcccact
    52381 agccatgtaa ataaacccgg caaataagcc gttgagtaaa aataagacga caccaatggc
    52441 aatcactaca ttttcacggg tatttaaaac agttttctta gccaagcggt aggcatgaac
    52501 caacgattca aaattagatt gcatcaagac aacgtcactc gtttcgatag caacatccgt
    52561 tcctgatccc atagcaatcc cgatatcagc cgcagccaaa gagggagaat cattgattcc
    52621 atctccaata aaagcaacgc gcaatccttg ttcttggtat tttttcacaa attgaacctt
    52681 ttcgtctggt aataattcag catggacttc atcaatgcct aacatatcag ctacataatc
    52741 agcagtcatc tgattatcac ctgtcagcat cactaactgt tttgctcctg cttgtttcaa
    52801 cttagctaat tgttcagcga cttctggtcg aatcacatct gacacaccta tgatttgagt
    52861 gatttttttg tcgattgcta caataatgac cgagctgcct gtttgttgta attgatgtaa
    52921 atcttgtact tgctttggtg tgagagaaag tttgtgagcg gcaatcaatt tggtgttgcc
    52981 cgcacaaact gtatgaccat caatctcagc tgtgattcct tgtcccttga ttgtctgatt
    53041 atctttgact gctaattgtt gaaaatcaat gtctttctcc tgagcataat ggatcacggc
    53101 gcgccccaga ggatgatcgg ataacgtttc taatcgagcc actaaagcta agctgtcccc
    53161 ttcatcctct gtatagttct tgatagtgga tacagcagta gttcctttgg tcaatgtacc
    53221 agttttatca aagacaaaag tatcgatttt ggaaaaagta tccattgctt caccgccttt
    53281 gactaagatg ccccgtttcg ctccattacc gattcctgct acattcgaaa caggcgcacc
    53341 aatgactaaa gcgccaggac aaccaagaac caaaacagtg attgctaaac gaaaatcacg
    53401 actaatcaga ccaacaacca acgcaataag taaaacggtt ggagtataat actgagaaaa
    53461 tcgatcaatg aatttttccg catgggattt ggtatcttgt gcttcttcta ctaattcgat
    53521 aattttggca aaggtcgtat catctccgac cttagttgct tcaatcgtca cgtagccatc
    53581 tgctaaaatc gttccagaga agacttcatc tccactgatt ttgacgactt gtcgtgattc
    53641 accagtcact gcggcttcat ccgcataccc tttcccgtca atgactcgtc cgtccacagg
    53701 aatagctcca ccagttttta ctaagacgat atcgccttct tccacgtcat cgatttccac
    53761 ttcttcagtc gttccatctt cttggacaac actagcagta gatggggcca tctcggtcaa
    53821 agccttgatt gactcccgcg ttttggtcaa tgttttttgt tccagatagc taccaaacaa
    53881 aaacaagaat gtaacgatag ctgactcctt gtattctccg atcataaagg caccaagcac
    53941 agcaatagta acgagtaaat caattgagat aactttgact ttcactgcct gatacgcatg
    54001 gatcataata ggtatgaccg caataataga agctatggct agagcaatgt tatagaacaa
    54061 gacaaaacca gtaaattttc cgataaaacc aaaaataatc aacacagctg ataaggcggt
    54121 gatatgattt cgatgctctt ggatccactt ttgtaatttc atctttctcc ctcttttcct
    54181 ttcattctta cgagactaag tatagatact tatccatcat aaaaaattga ccactatcaa
    54241 gtttagtcaa aaaggcggtt ttcttttggt agaatacact acaaaaaata gttagcaaga
    54301 agactcacta aaagtgatct tgccaactat tgtcgtttct atcgaaattt tcattgaatg
    54361 attttcctgt acagcataca tcggagatct atttctttat ctaagatttt tatcttgcta
    54421 attttttcct ttagtctttc aaatccacgt cgttcataga attgatacgt acagccacta
    54481 tctgtaaaaa ggaatatttc ttttcctttc tctctttttt caaattctgt tagcaacttg
    54541 gtaccgatgc cttttgtttt caaatcagga tttgcagcta aaaaaatgat ctctccatct
    54601 ggcgaatgct tctttaaata tgctgcaagc atttgttcat ttgctgtctc gtaagctcct
    54661 ttactttctt tagcaaatat attttgtaaa aaatcgaata tcttaacata tatttgctgt
    54721 ccaaaagaac gatgtttctt tttttctcct tttatctccg ccagcaaaac acctgccaac
    54781 tcgtcctctg catacaaagc aagaatttga gttgctcgat tcaactccat atacaaaaaa
    54841 tatcttccgt aaagattcag cagaagttca ttttccatat atctctcaaa gttcattcct
    54901 tcaacagcaa aatagatagc ttttttgtaa tctttctttt ctaagggttt aaaagttagt
    54961 ttcatcattt cgcctcctca acttcaatga ttgtgttata cttacattat aatagacaca
    55021 gtgtctacta tctataatca atcatctata agtttgtcga ttaagcgaca gctttgcttt
    55081 gatatgtcgg ataaggagat aggtaaatga aaaagacaga tagacgcgtc aaaaaaacag
    55141 aaaaagcttt ggctgaaaca ctatccacat tattagtaaa taaaaagatt caagcaatca
    55201 ctatacgcga actaacagaa acggcagatg tacatcgttc gactttttat acacattata
    55261 aggatatcta tgatctatat gat
//
LOCUS       DANKBF010000010        54452 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000010 DANKBF010000000
VERSION     DANKBF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="PWY89_000637"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="PWY89_000637"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2065695.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="PWY89_000638"
     CDS             complement(1036..1956)
                     /locus_tag="PWY89_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2065696.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /gene="xth"
                     /locus_tag="PWY89_000639"
     CDS             2069..2821
                     /gene="xth"
                     /locus_tag="PWY89_000639"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2065697.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="PWY89_000640"
     CDS             3685..4218
                     /locus_tag="PWY89_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL2065698.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4708..5232)
                     /locus_tag="PWY89_000641"
     CDS             complement(4708..5232)
                     /locus_tag="PWY89_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2065699.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="PWY89_000642"
     CDS             complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="PWY89_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2065700.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5821..6801)
                     /gene="pta"
                     /locus_tag="PWY89_000643"
     CDS             complement(5821..6801)
                     /gene="pta"
                     /locus_tag="PWY89_000643"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2065701.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6954..7634)
                     /locus_tag="PWY89_000644"
     CDS             complement(6954..7634)
                     /locus_tag="PWY89_000644"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2065702.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7760..8629
                     /locus_tag="PWY89_000645"
     CDS             7760..8629
                     /locus_tag="PWY89_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2065703.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8655..9170
                     /locus_tag="PWY89_000646"
     CDS             8655..9170
                     /locus_tag="PWY89_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065704.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9217..9810)
                     /locus_tag="PWY89_000647"
     CDS             complement(9217..9810)
                     /locus_tag="PWY89_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2065705.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9835..10014)
                     /locus_tag="PWY89_000648"
     CDS             complement(9835..10014)
                     /locus_tag="PWY89_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065706.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10091..10600)
                     /locus_tag="PWY89_000649"
     CDS             complement(10091..10600)
                     /locus_tag="PWY89_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065707.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10696..11076)
                     /locus_tag="PWY89_000650"
     CDS             complement(10696..11076)
                     /locus_tag="PWY89_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2065708.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11406..12155
                     /locus_tag="PWY89_000651"
     CDS             11406..12155
                     /locus_tag="PWY89_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL2065709.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12476..13693)
                     /locus_tag="PWY89_000652"
     CDS             complement(12476..13693)
                     /locus_tag="PWY89_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2065710.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(13951..15228)
                     /locus_tag="PWY89_000653"
     CDS             complement(13951..15228)
                     /locus_tag="PWY89_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065711.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15307..15951)
                     /gene="pcp"
                     /locus_tag="PWY89_000654"
     CDS             complement(15307..15951)
                     /gene="pcp"
                     /locus_tag="PWY89_000654"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL2065712.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16150..17094)
                     /locus_tag="PWY89_000655"
     CDS             complement(16150..17094)
                     /locus_tag="PWY89_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL2065713.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17096..17782)
                     /locus_tag="PWY89_000656"
     CDS             complement(17096..17782)
                     /locus_tag="PWY89_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL2065714.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18256..18576
                     /locus_tag="PWY89_000657"
     CDS             18256..18576
                     /locus_tag="PWY89_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL2065715.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18730..19635
                     /locus_tag="PWY89_000658"
     CDS             18730..19635
                     /locus_tag="PWY89_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2065716.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19632..20405
                     /locus_tag="PWY89_000659"
     CDS             19632..20405
                     /locus_tag="PWY89_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2065717.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20586..22010)
                     /locus_tag="PWY89_000660"
     CDS             complement(20586..22010)
                     /locus_tag="PWY89_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2065718.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22221..22667
                     /gene="nrdI"
                     /locus_tag="PWY89_000661"
     CDS             22221..22667
                     /gene="nrdI"
                     /locus_tag="PWY89_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2065719.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22732..24714)
                     /locus_tag="PWY89_000662"
     CDS             complement(22732..24714)
                     /locus_tag="PWY89_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2065720.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24728..25624)
                     /gene="murQ"
                     /locus_tag="PWY89_000663"
     CDS             complement(24728..25624)
                     /gene="murQ"
                     /locus_tag="PWY89_000663"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2065721.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25627..26688)
                     /locus_tag="PWY89_000664"
     CDS             complement(25627..26688)
                     /locus_tag="PWY89_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2065722.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(26899..27465)
                     /locus_tag="PWY89_000665"
     CDS             complement(26899..27465)
                     /locus_tag="PWY89_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2065723.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(27493..28995)
                     /gene="eat(A)"
                     /locus_tag="PWY89_000666"
     CDS             complement(27493..28995)
                     /gene="eat(A)"
                     /locus_tag="PWY89_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2065724.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29253..30374)
                     /locus_tag="PWY89_000667"
     CDS             complement(29253..30374)
                     /locus_tag="PWY89_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2065725.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30500..31441
                     /locus_tag="PWY89_000668"
     CDS             30500..31441
                     /locus_tag="PWY89_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2065726.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31591..32487)
                     /locus_tag="PWY89_000669"
     CDS             complement(31591..32487)
                     /locus_tag="PWY89_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2065727.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            32756..34096
                     /locus_tag="PWY89_000670"
     CDS             32756..34096
                     /locus_tag="PWY89_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2065728.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            34314..34757
                     /locus_tag="PWY89_000671"
     CDS             34314..34757
                     /locus_tag="PWY89_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2065729.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(35261..36679)
                     /gene="nhaC"
                     /locus_tag="PWY89_000672"
     CDS             complement(35261..36679)
                     /gene="nhaC"
                     /locus_tag="PWY89_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2065730.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(37082..38305)
                     /locus_tag="PWY89_000673"
     CDS             complement(37082..38305)
                     /locus_tag="PWY89_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL2065731.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(38336..39175)
                     /locus_tag="PWY89_000674"
     CDS             complement(38336..39175)
                     /locus_tag="PWY89_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2065732.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(39192..39872)
                     /locus_tag="PWY89_000675"
     CDS             complement(39192..39872)
                     /locus_tag="PWY89_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065733.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(39874..40947)
                     /locus_tag="PWY89_000676"
     CDS             complement(39874..40947)
                     /locus_tag="PWY89_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2065734.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(41003..41241)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(41284..41517)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(41594..42265)
                     /locus_tag="PWY89_000677"
     CDS             complement(41594..42265)
                     /locus_tag="PWY89_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2065735.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(42352..45030)
                     /gene="mgtA"
                     /locus_tag="PWY89_000678"
     CDS             complement(42352..45030)
                     /gene="mgtA"
                     /locus_tag="PWY89_000678"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL2065736.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            45689..46126
                     /gene="ndk"
                     /locus_tag="PWY89_000679"
     CDS             45689..46126
                     /gene="ndk"
                     /locus_tag="PWY89_000679"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2065737.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(46148..47014)
                     /locus_tag="PWY89_000680"
     CDS             complement(46148..47014)
                     /locus_tag="PWY89_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2065738.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(47174..49267)
                     /locus_tag="PWY89_000681"
     CDS             complement(47174..49267)
                     /locus_tag="PWY89_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2065739.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(50016..50639)
                     /locus_tag="PWY89_000682"
     CDS             complement(50016..50639)
                     /locus_tag="PWY89_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065740.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(50798..52195)
                     /locus_tag="PWY89_000683"
     CDS             complement(50798..52195)
                     /locus_tag="PWY89_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2065741.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(52227..53198)
                     /locus_tag="PWY89_000684"
     CDS             complement(52227..53198)
                     /locus_tag="PWY89_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2065742.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            53572..53997
                     /locus_tag="PWY89_000685"
     CDS             53572..53997
                     /locus_tag="PWY89_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065743.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        15718 a        11284 c         9619 g        17831 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttaccaaga ggttgtgaaa
     4501 tcagcgtttt gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc
     4561 gttttttgag ctaataccga aggtcagctg attgaacacc gtttattcaa aaaaaatgga
     4621 ctgcgacacg acttttgtca cagtcccttc gtgtgttttt ctgccttctc aaaaggatga
     4681 cagtttctca ctcttttcct atttcttcta ttcaatcaaa taactcattt tatatacatc
     4741 taaaaattca cctagatctg ttcttgcccc tctaggcatg accgcttcga tttggaagcc
     4801 catttttcgg taaaggtgta cggctctttc attacgaact tgaacgtcaa gctccaatcg
     4861 aaaaagtaca cccatttcct tcgcccaact gatgatttct tctagcataa gagtgcctag
     4921 tcccattccc caatattcct gcagaatact gataccgact tctcccacgt gagacaaacg
     4981 aaattgactg tctgccttga cagatgctgt tccaataatc gtcccttcat agattgcaag
     5041 cagcaataaa ttattattgc tttcgtatat ataatccaac tcttcttcta aggtttctgg
     5101 tggtaacagg agttctgctt catccaaaac taaaaattct gtctccttat taacttgtcc
     5161 catcatcaaa agaatgtctt ttgcatctcc tggcactgct tcccgcaaga tgatcccaac
     5221 ttcttcactc atgattttca gcccattttt ctagaattcg ctgtttgaag gtttcacttt
     5281 gctccccgta tgagattagt ttcacatacc gcttttctaa atctgtatca cttttttcta
     5341 aaatcagttc caaataatcc tccggcagtg cttcttctaa tagatttccc cattcaacaa
     5401 cggaaagccc atcaccttca aaatattcat ctaaccctaa atctgctccg ctagctgcta
     5461 cccgataaat atccatatga tacaacggaa tccgaccttg actatattct ctaataatcg
     5521 tgtatgtcgg acttttgatc atttgcgtta tccctaatcc ttgggcaatt cctttcgtca
     5581 acgttgtttt acccgcacct agatcaccag tcaatatcag attgtcacct ggcattgcgg
     5641 aatgaccaat tgcctctgcg aattgttctg tcattgttaa tcctgatagt tcgatcattt
     5701 ttcatcttct cccgctcttc tttctttttg ccagtatatc gcgtaatgaa gtttttttaa
     5761 agaaaaaatg aactagtcaa gaacagcagc tgctgttttt ggctagttcg cgtgtatttg
     5821 ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat ttatatacat cttcttcatt
     5881 tgctccacga gacaaatcag aaaccggttt gttcaagcct tgtagaattg gtccgattgc
     5941 ttcaaagtta ccgaaacgct gagcaatttt atagccgata tttcctgact gcaattctgg
     6001 aaatacaaag actgttgctt gaccagctac ttctgaatct ggcgctttca gttgtgcaac
     6061 tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag
     6121 ttttttagcg attttagttg cttcaactac tttgtctact tctggcgatt tcgctgagcc
     6181 ttttgtcgag aagctcatca ttgctacttt tggttcgata tcgaacaatt ctgctgtttt
     6241 tgcagaatct acggcgattt cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca
     6301 atccgaaaag acatattttt cttgatctct tccacgtacc atgatcatcg ctccgcttgt
     6361 ccgactgatg ccaggctttg ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt
     6421 cgaatggatc gctccagaga ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc
     6481 aaagtaattc acatctttta aaatcttttc tgcttgttct ttggatgcct ttccatttct
     6541 acgttcaaca aaagtatcca ccatttcatc ccacttatcg taattatcag ggctgatgat
     6601 cgtaaagcta gatgttttga ttcctctggc atgcgctgca tttcttattt cttcttcgtt
     6661 ccctactaaa atgggttcca caagttcttc tgctttcaac cgggcagctg cgcctaaaat
     6721 acgagcatca gtagcttccg ggaaaacgat tttgatccca cggcgaacaa ttttaaatct
     6781 taaactatca aacaattcca caagatgacc tccagacgtt tttatatgta ttatcataca
     6841 ctattccaga aaaaaagaac agtttatttt atctgtatca gtcaaaaatg ctggaaaaat
     6901 taagtttttc acatacaaag cgaactgcac tataacagga aagcgctgtt ttattacact
     6961 gtttcaggca gttgccaatc aatcggttct tctcctaacg caaggagcgc atcattcgtt
     7021 ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag ataacggact gggatgtgga
     7081 gatgttatga tgatatggcg actcttatcg atcattttga ttttttcttg tgccggtttt
     7141 ccccacaaga taaacacgat tggtttttcc cgttcattta atttttcaat gatcgtgtct
     7201 gttaatcttt cccaaccttt cccgcgatga gaatacgctt gcccttcccg aacggtaaga
     7261 acggtgttga gtaaaaggac tccctgttta gcccatgaca ctagatttcc atgttgaaca
     7321 ggtgcgattc caagatcatc ataaagctct ttatagatat ttcttaacga tggcgggatc
     7381 tttacgccag gttgcacaga aaaagataag ccatgagctt gattttctcc atgataagga
     7441 tcttgtccta aaatcaccac tttaacttct tcataaggag tcaattccaa tgcttcatag
     7501 atatgataca tatctggatg gatcttttgg gacgcatatt ctttttttaa aaattctctt
     7561 agatgcagat aataatcttt tgaaaattct gaggacaaaa cttcttgcca actgttatga
     7621 atgatttctt tcaaaaactc cacctcctaa taaaaagaat aactcacatt ttcttcaatc
     7681 acaagggaca atcttcaccc tcagcacttg attaatatgg taaactagga acaagaagca
     7741 gaaaggaacg ataatcatta tgatcaaatt aattgcatca gatatggacg gcacattatt
     7801 agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc
     7861 cggtgttgag ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg cagctttaga
     7921 tgaggtagga atcgattgtg cgatgatcac tttgaacggg gcacaggtat ttgataaaga
     7981 aggaaattca ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg ttttagatat
     8041 tctagatgct aacggtattt actatgaagt agcaaccaac gatggtcttt attctgaaaa
     8101 tcaagcgaaa cgaattgaaa gttttgcttc catggtggct acccatttac ctcaccttac
     8161 gtataaaatg gcgattgcca tggcctcagc taagctggag ttattgcaca tcacatacgt
     8221 ggacagtatt cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt
     8281 tcatacagaa gggccagctg tattaggtcc agttggaaag cagatttctc ttttaggcga
     8341 tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa
     8401 aggaattgct gttgcccatg tcgctcacga gcgtgggatt tctttagaag aagtcatgac
     8461 gattggcgat aacttcaatg atgtcagcat gctgcaagta gcaggagtca gtttcgcaat
     8521 gggaaatgca gaaatcgaag taaaagatta tgccaaatat acaacggaca caaaccttga
     8581 atctggcgta ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt tttatctaga
     8641 aaagagggat atagatgtca gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc
     8701 ggacaattgt caaaaacgaa aaaggacatt ccctgttttt gatggttggt cgctggggaa
     8761 caaagggcga tgcattgtct ctttatgcca tgaatggcga tctcgttgct cacatcaagc
     8821 agataagcct gacattcggt acgcgttttg agctgtataa agagttcgaa aaggtgggaa
     8881 ccatgcagaa aatattcaat tggcccggtg acttttatta tattcgtaga cttcactgga
     8941 cagtccaagg tgatatctat aatcatcaat atcaaatcca tcatttcaat cagcaaatca
     9001 tgaaaatgga taaagcaaca cttttttcag gagattacta tgtattggat gtctcaaatg
     9061 atgaagatgc tccagtttgt atttgtattg ctgcggtttt agattactgg ctctaccgta
     9121 aagacaaaag tcgcgataag cctgtttctt accgacttaa cttaagttaa aaagatgtac
     9181 ctgaaatgcg gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc
     9241 ataaagatga ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa
     9301 ttttttagcc tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt
     9361 cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc
     9421 ttctgataca ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa
     9481 caaacgtgta gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat
     9541 gattgattct tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc
     9601 ttctacgata cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa
     9661 aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag
     9721 aatcactaag atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat
     9781 agtttcctct ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt
     9841 taaaaaatct tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc
     9901 agttcggcaa taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt
     9961 gacttcttcg tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc
    10021 tcaacttcct ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa
    10081 tatccttctt ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga
    10141 ctttataata aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt
    10201 tcccgtagta gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt
    10261 tttctatatc aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc
    10321 cagtggatgg attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg
    10381 cattcgtagg attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt
    10441 cttttgtttc gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt
    10501 cactaatatc atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta
    10561 ttacgatgag ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt
    10621 ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg
    10681 acaattttgt tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa
    10741 cgtcattttt tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga
    10801 ctgcaattct gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc
    10861 acaagcttct ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc
    10921 agcacttcct agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct
    10981 gattacttca aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac
    11041 aatgatggct atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta
    11101 agctcggatc tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc
    11161 tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac
    11221 tcttcctgtt cccaatataa taaatgaaag taagaaatta tattttcaag aaaacaaaca
    11281 gaaaattatc ataacacaaa ttatttataa ttgaaagact agtttttttt caagaaaagc
    11341 atacaataga ttgtgttagt aaatacagaa cacactacat ttagtgtctt aaggagtcct
    11401 catacatgaa gtcatatttt cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa
    11461 attattatct tttctttttc agtcttggct gccaggtcat gactcttgtc aatagtccca
    11521 tcacttggct gtccgttatt acatttatcg gtaccacgct tggtgtattg tgtattttat
    11581 ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg atttgtttta
    11641 ttatcgttgg gttttcagca aagaactatt taagtatcgg tgaacagatt gcttatatga
    11701 tcacattgga tatccccgta ttgttaagta aagaatggaa tcataatatg gcttcaaaaa
    11761 tccgaaaatt cactggaaaa agctggttta tcgcaatcat cagtactttc cttgtctatc
    11821 ttgtttcagg attcctgatt ggtcgttata cagatgatcc tcggccgtgg gtagatgcca
    11881 ttgctttttc tattagcttg actgctggta tcatcagttt cttgcgttac aacaaccaat
    11941 attactggtg gttagcttca gggcttgctc aaatggtcct ttggtatatt tcatttacgc
    12001 atggatctgc aagtttggcg atgttcgtga acagctctgt ttacttgctg aatgatgtcc
    12061 ttgcatttac cgtttcacct tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg
    12121 aagaagaata ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta
    12181 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaatcaga agcaactcct
    12241 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    12301 ttcttggagt taaacgcttc tgtcccagcc tctatttcct acgattagtc aagtaaaagg
    12361 tgcaagaaaa atagctcgta aacgaaagcg acaaaatcaa agcacaccaa ataaagcccc
    12421 gttcgtaaaa aattttcgat ctgggcaaag aagtggtata ctgtaagtgg tttagttaat
    12481 ggtccactga gaccgcgtac gcatagtcgt actctgtatg tgccctaacc atagcgtgca
    12541 tacatttcag aagcttgttc atacaagcaa ctgtggcaac cttatctttc ttagggatag
    12601 gttgcttttt taatttgtag taataatcga caatatgatt aggagctgcg cgttgctggc
    12661 gaatcatatt tcgaatggtg aagaaaataa ttttccgtgc tttgggatta ccacgcttat
    12721 tgatgcggtc ttggccagta tacttacctg aatgataccg tcggatgtcg attccgacaa
    12781 aggcattcaa ttgtttatgg gttttaaatc gtgtgatatc tcctaattca ccgagcaata
    12841 aagcggctgt gagtgcgcca atcccaggga acgtttggta caacaaaaag caaggagagc
    12901 cttcggcttt tttgatcatt tgtgtcgcca gtgctttttt ttgttcgagt aaatcttgga
    12961 gaagttcggc gtaatagatc gttttttgac agtggatact gtctttttca acagctggat
    13021 aggcacactg tgcatagtgg atgatttgat ctgccttttg ctcggcacga ttcgcggaga
    13081 tttttttccg tgtttcgttg atcagtttat tttttatttt tgtccgagta gatgccaata
    13141 caaaatctgg atggggaaaa agacggatca atgtcaatgc gtaaggggtc agcctgtttg
    13201 aaaagaactg ttcaagctct ggaaaagtca attgaaggca gttgtgtaaa tccatgcgca
    13261 gacgagtgat tttcttctca atttcttgat aaaaacgggc taaatcgcgc atctctaagt
    13321 aaaaattgct ttgtacttct tttggctgac gttgattttt aagatgagtc tgtgcgagcc
    13381 ggtgggcatc cgatttatct gttttccaac tgcgtaaggt gctttcttcc atctgttttt
    13441 tcgcctctaa cgggtttaac aaacagtaag aaaagtgatg atcttgaaag aacttttcga
    13501 ctggtctgga ataaatcccc gttgcttcaa aaacaatcaa tggacgctca ggcaagtgct
    13561 ggatctcttc gagtaatgca tgaaatcctc tttgagtgtg ttcaatctcg tcttcaaata
    13621 agcaggtgtc gtctttgtat aggacactgt agctgtgtcc cattgaaaca tctaatgcaa
    13681 gaatgtaagg catttgtttt cctccttttt aagttttgaa agaactttac tgcttcattt
    13741 cgattccaat ttcctataca cgggctcatg gccccaacat acgtaaacct gattctaaaa
    13801 tgacagtaaa gaagttcggt ttttgatagg gactcgtagt cctcaaggct ttgttcgaac
    13861 tttctttcac tcttcactat aatctaaaaa tagaaaatag aggagaacat cattcgtcaa
    13921 tgatgttccc ctctaatctt agtatgtttt ttatagtaat tccttcagat actttcttgc
    13981 ctcatcattg aaacgcagtg attcttctaa cttctgataa tattccaacg cttgtttctg
    14041 agcttcagta aaacgataat gttttccttt ccatttgtcg attgtctctt gcacatttcc
    14101 agattggata tctttcacta ctagttgcca tttttctttt gagactgtac ggtctttctt
    14161 atcagcagat tgtatagctt ctcgttcttt acgcaatact tttaaaaaac catttctgac
    14221 tatccgagga atgctgtcga agaacccttc tgtaaaacca tagcgacaat attgcttcca
    14281 aataaataga tagatttttg gttggatgaa tcgttggttt ttcgcaaaat attccatctg
    14341 agcagtgact tctggtgttc gttgacgttt tgtctttctc aacacggaaa taaatgtttc
    14401 aaaataagca ttaggatgta ctggcacttc ttcatatacg atggattgcc aatctttacg
    14461 taaaaatgcg cgatagttga tcaagaattc ttctacatcc tttactaatt cttctgcaaa
    14521 gtcgtcaaat agttcttttt ttatctgtcg cttttttgac tgcccattga tgtgttcact
    14581 tattttcttg atttcatttt ccaatgcttg ataatccgct atgtaaatcg gcatttctac
    14641 ttggcgaaat tctgcgagcc gctgagctaa ttttatatcc ggtaaatatg gctgtttcat
    14701 ccgacgattt tggtacaaga caaaacagta tttttgccat agtccttcag gaaaatcgat
    14761 attcttctgc ttaagccata ataattcgtc taaggccaga tcaacttttg tcattccctg
    14821 atggatcgaa gctgccactg tcggagagac atgcaggtga tcggcaaagt ggttgatgcc
    14881 tagttttagg atctttccgg tttttggcga tttaacgatg tattgcactt ttaattctct
    14941 accacactca caatacaatt tttgctggct tcgttttgtt cgaaaaaacg gattgatctt
    15001 gaagtcagaa aacacccaat ctgttgcagc taaataatgt tcttccataa aacgagagct
    15061 gattagatat cggcggtgct tatcgactag ttcccgctgt tttttcgtta attttgcata
    15121 gatctcccaa cgttgactgc ttgtgtattg tttagaaatt ttgatgggat agcttttgct
    15181 gatttctttt ggaaaccatt cttcaaagac ggattgccat tcttccacgg ctgtttcttt
    15241 cctttctaat tacaaaaagc tggctactaa cataacgtta gcaaccagcc agtgtttctt
    15301 gctaaattag tgagttgttc cggcatttcc ggaaatatct tctttatttt ctactgctgc
    15361 ttcaactgca taagtcaaag aagaagcaat gtcttctaaa gacatagaag gcgtacccac
    15421 tggacgatcg atcacttgat cgggtgagaa tggcacgtgg atgaacccac ctcgaattgc
    15481 cgggaattgt gtttcgatca aatgaagcaa acgatacatg atatcattgc agacaaatgt
    15541 tcctgctgta taagagatgt acgctggtag accatgatct tgtacatttt ttgtcattgc
    15601 tttgattggc aatgaggtaa agtaagcggc tggaccgttt tcttctaaag ccgtatcgaa
    15661 tggctgattt ccttcattat cagggattct tgcttctgct agattgattg cgacacgttc
    15721 gaacgagact gctgaccgcc cacctgcttg tccgacacag atcacgacat ctggttggtg
    15781 ctgttggatg gcttctttga ctacttgact gcttttttcg aatacggtcg gtacttccac
    15841 tttgatgatt tcagcaccgc tgatttcatc tggcattttt tttactgctt cataggctgg
    15901 attcactttg tccccaccaa atggatcaaa acctgttact agtactttca tctgttgttc
    15961 ctccgtccga ttgatttaac atatggttat cttttatcat accattttcc ttgaacagcg
    16021 taaataaagg aaacaatagt ttaaataaca taacagatca aacttaatat attcttataa
    16081 aaaaatagtg ttccactaca atgttagaag aggttgcgac aattattctg tcacaacccc
    16141 tctcttctat tatttaaata gaatcatata agcaatttgg aaaactagca taaataacgc
    16201 aataaacaac tggtttttga tcactccgta tttgtctttc atctccagca tcgctacagg
    16261 cacgatattg aagttagcag ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa
    16321 tgcaaccatt cctattaacg ctggattcgc tccatgacta aatacgaatg gtgcaccgat
    16381 cccaaccgtc atcacggtga tggccgcaaa tgcattcccc atgattaccg taaatatcac
    16441 catgccaaga gcgtaaatga tgatgccgat gttcacattt ccttctggaa cgatcgtacc
    16501 tactgcattg gaaatcactg tcccaactcc agcgcttgta aataccgcac ctagagaagc
    16561 taaaagcatt ggcaacatgc ttaacggccc tactgtgccc agcatatctg ctgcatcatc
    16621 taaaaatgta cttggtcgat tatctcgaga aaagaacatc aagattagca tggctgcaaa
    16681 aacaccgacg cccacgccga ctaaagcacc taaattggta aatagagcaa acaagatcgc
    16741 aaagacaccg atactcaaag ctggaataaa aattttcatt cctagtttgt ctgacatttt
    16801 ttccatatat tcttttgatg gaagccggct ttctcctttg ctgactttct tagcaattgc
    16861 tggtatggtc atcgcaaaga tcaagccacc gttcacgatt gccggaagcc atcttcctcc
    16921 tgcaataact acccctaaga ccgtccagaa aaaggctgac ccataacgat gaggattggt
    16981 tttatctgtt aaatttctta ctccagtgta aatcaatacg agtcccatca cgatataaag
    17041 gatctccagt aatttatcgc ctaataaaat atcaggattt gtaaaaaagt ccatcttatt
    17101 gcccctttcc tggataatat ttcttcgcta atttcttttc tttaagatag aagtagacac
    17161 ttgccacaac taaagccgtg atagccactg gaacttccac taaagcaagc tgtcccaatg
    17221 ttacgtcata tcctagatct ttcagtgttg attggactag caacgcccct gctcctccga
    17281 tgaatagtac ttggccaaag aaccagcaga cattttccat agctgaagcc atacctttta
    17341 gttcttcttc atgcttagga tggacttcta acccttttga ttcgatggtc cccaatgcca
    17401 tcggtaaaat gattggacga acaaatccag caactccacc aaaactcacg ttaaaagccg
    17461 caaagatcat tcgaaagatc ccataaatat ctacaatcat accagcagat actttcttga
    17521 atcgcttgat caaggaagcc gctgattctt tcagaccatt tctttcaagc gttcctgttg
    17581 ccaacataat gatgataaaa atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa
    17641 gttctaacat tccttgaatg ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca
    17701 ttacgatcaa tatcgagtct aatttaaatg caaacccgag aataacaatc aagacgccta
    17761 aaaggctaag ataattttcc atatattttc ctccataaaa tgacaatatt tttttaatta
    17821 taaagcaaaa cacataaaaa atatagttgt tttctcacga aatagacact atttttaaat
    17881 atttttcgtt atttttttat cacaaaaatt atcttactta ttgtaaggta aagctttttt
    17941 taatttattg cccgataata tatagaaata aggtctagtt actgtttttt ttaaatgatt
    18001 aataaataac aacttctttc tatgaaattg caaagcattc atcttctttt ccgctaacga
    18061 tatatccgtc agtgcttggt acactttact aaaaagtatg agattgccga tatctggata
    18121 ggtgtgctat actacagaaa aagatataca atctgccacc gggtaggata aagaggatga
    18181 tcattccatt aggtctttga agggatgatt ttcctctttt tgtgtatttt tttatagtta
    18241 aggaggaatg taagtatggc atggatggaa ttaatcatag caggaatttt ggaagtattc
    18301 tggtcaacga tgatgaaatg gagtgatggt ttttctaaaa tcaattattc tttttacaca
    18361 gtgattggca tgattgctag tttttacttt ttgtcaaagg caatcaaaag tttgccgatg
    18421 agtctctctt atcctatctg gacagggatt ggcgcagttg gttcggtcat aattggtgtg
    18481 gtcttgtttc atgataagct gaatctttcc acttggttct ttgtggggat gctactcatc
    18541 agtatcattg gcatcaagat tactagcggc cactagacac ttggaataag tactgatcag
    18601 aaaaaataag cctttttatt taatattcaa tttttttatg aaaaaataag aaataagtcc
    18661 aatgacctag cgacttctcc actcatcagg tgtatgcttg tactatctat agaaaaggag
    18721 tgaatatttt tgaaaagtct ttcggtccaa gaattatcca agaactttgg tcacaaacaa
    18781 gccctcagcg atgtcagttt tagcttatcc cctggcaaaa tcgttggatt agtaggtcct
    18841 aatggcgctg gaaaaacaac gatcatgaaa gctattttag gactgatcca ttactcagat
    18901 ggaacaatct gtattggtga aaatgagatt tctccctcta gtcataaagg gttggaacaa
    18961 gtaggagcat tgatcgaata cccgggtatc tatccattct taacaggcta tgatcatctg
    19021 aagttattct ctgaaaccaa cgatgtatct gctatcgaca ctatcgtgaa tcagctcaag
    19081 atggaaaagt acattcacaa aaaagcgaaa tcctactcgt tgggaatgaa acaaaaattg
    19141 ggaattgctc ttgccttact gaaccatccg tctatcgtta ttttagatga gccgatgaac
    19201 ggtttggatc cacaagcaac aagagatgtg cgaaaaatca ttaccgactt ggctactcag
    19261 ggcacctctt tcttgatctc cagtcactta ctcagcgagc ttgaaaaaat ggtcgatgag
    19321 ctgatcctga tcaatcaagg aaagatcatc aaacaatgta caatggctga attgaaccaa
    19381 acagcgcaag atttcatcgt tatctctaca acaaatgatc tagctgctcg aacgatcttg
    19441 acagaagctg gatatacttt ggaagatcct gataaaatca agcttttaaa agaaaacgaa
    19501 gaattattag gaaccgtgat ccagctattg tctgcacaaa atatccatgt gacagacgtc
    19561 cagcatctga cgaatgattt ggaatcttct ctattggaaa ttctggattc agtaaagggg
    19621 gaagaagaaa aatgacattg cttgtaaaac aagaattatt taaattgatc aagaaaaagt
    19681 caactgctgt tttatctgtt ctattagttg ttttgctcat aggaacagca cttctagcca
    19741 aaaaatacac gacgatcatt gatccagttg aaatgactgc tcagcttttt agtgccacat
    19801 cttggattgt ctttatcatg attgcagctg caagcacgat catttcgatg gaagcacaat
    19861 atggtacatt gaaaaatctc ctttatcgaa agtattctcg aggagaaata ttagtaagca
    19921 aatggatcac attagttatt tattctgttt atttatacct tttagctatt attgtcactg
    19981 tgttgatgaa actgattctt ttcccttcga tttccttcac ggagaaggtt tctactggtc
    20041 aaacactgat ccaaagcttg ttcacttata ctttgggtag ttatatcggt ttatggctga
    20101 ttttaagttt agtgttgatg attgcttgct ttatcaatag ttccggcgca tccatctcag
    20161 caggaatcgt cttttatttt gcttcgtcaa tcatatctgg tatccttatt gcactcatcc
    20221 aaaaatggga gtggatcaaa tggaatccga tcagtatgtt gaatctgcaa aaccaaattg
    20281 gaaacgaaga gatcatgaag caattgactc atctttctac taatcaaatg ctattcggta
    20341 atttagctta catcgttttg ttcttagcat taggatatct cgtatttaag cgaaaaaatg
    20401 tatgatgact catcaaaaaa gaggctgtga aagagacgtt cagccttatt gccgattggc
    20461 tgtctcttaa acaccgttta ttctgaaaaa ggagctgtga caacatttgt gtcacagctc
    20521 ctttcctagt atgtttttga ataaacgaca ggctcacaac ctctctttat acttctttta
    20581 tacttctata cgtgaagcat cccagtggca aataaaacca ttgcaatcac tccgaccatc
    20641 gtcacaccta taagtaaaat cttatctaag tggatatgtt gtacaggctc gttttgctcc
    20701 ttacgtgctt tgaggtaaaa ataaatgcct ggcaagaaaa gaacaagaac aagcattact
    20761 tcattaaagg ctacgactag catagcccat acttgaaagg cacttgctaa aagccctact
    20821 aaaaattgga cccattcttt ccgctgataa gaaagtttca tttggtacaa tcctaagaaa
    20881 agccacgtga agaaaatagc tgctgtacca agtgtgtaag caaagttata agcactgtca
    20941 gtgaataata atgaaagtaa aaagacattc gtacataacg tcgtcaatac gatagcataa
    21001 gttggtgccc catttttgtt gagttttccc catgctttag gcaaaagtcc gtcttcagcc
    21061 atgtttccca ttgtttcaat cggtaagatc gtccatgaga gccaaacacc aactaaagaa
    21121 atgatcaaac cgccgttgat cacgactgct ccccatttac caaccatcat ttcaaacaca
    21181 tagcccatag caggctgcgc cttgatcgtt tgcagctgat tcatcgtcat tgtcccatat
    21241 ggaagcatgg aaaccaagac gtaaataata atcaagccga atatccctag aatcgttgct
    21301 ttcccaacat ctgattgttt ttttgctcgt gaacctacga cagaggctcc ttcaatacca
    21361 acaaataccc aaagcatcac catgatgcaa ctcaatactt gactggaaaa cgggatcggc
    21421 tgtccattcg cattcaatat aatgtcgcta gaaaagttat caatgaattg ttggatcgaa
    21481 aacgtgctga tagctactac aataaataaa aataatggca ctaatttaca gaatgtgacg
    21541 atagcattga cgaaactagc attttcgact ccccgattca ctagccatgc atatccccat
    21601 aaaaagactg tactcatcac gatcgatgcc agattttgcc caccttcaaa tactgggaag
    21661 aaatatccta atgcgctcat caacaatgtt gcaaaagcta cgttgcctaa ccagcatgtg
    21721 agccagtaac cccaaccgct gataaaacca gcgaaacggc caaaaccttc ttctgcgtaa
    21781 ctaaagatcc cgccttttaa atctggacgt tttttattga tattattgat cgacaaggag
    21841 agcatcaaca ttgctgttcc tacgagtacc caactgaaaa tagctgggcc gccagccgat
    21901 ccggagatgt ccgtcataat accaaatacg ccacctccaa tcgctgaacc aattactaag
    21961 gcagcaagtg gaattaaacc aattcctttt ttctgttgct ctgtatccat gtaagcatcc
    22021 tccaaaataa aaccgctttt atcaaaaagt ataacaaacc ttttttattt ttacttaatt
    22081 aataaaaact ttaatagaaa tataattcta ccggcttttt tctcattata caagcaaagc
    22141 gcctagtccc tttccaaatt acaccaccaa aaaacttaaa aaagatgcta tactaaaagg
    22201 aaagaattgg aggatgatcc atgaacattt tatatatatc gatttcagga aatacgcgtg
    22261 cctttgccaa acatctagca gaatatgctg aaaaaatgca tgcagaagat cctgtcaatc
    22321 ctgaagttac tttaaaagaa atccatgaaa atagtgattt tacgaaagaa acagaaccat
    22381 tttttacttt cgttcctact tatttagacg gcggaaatgg tttagataac ggtgatacag
    22441 aaatcttgac cgaaacgatg cgtgaatatc tggaacatga agacaatcat aaactttgtc
    22501 ttggtgtagt cggtagcgga aataaaaact tcaataatca gtattgtcta acagccaaac
    22561 aatatgcaca aacatttggc tttcctttct tggcagatta tgaattacgg ggaacaccat
    22621 ctgatgtgac acgaatttat cagattttag cagaaaacaa taaataataa aaaagaagaa
    22681 gctgaaaaat agatttcagt ttcttctttc gtttctattg tttctatcat attaagcttt
    22741 gacatccatc acaggtgctt ttgcttttgc tttacctgtt ttttccaaga caaagttttt
    22801 tacacgatcc atattggtta ccaagacgat catggatgtt tctttgcctg ctgctttgat
    22861 cgcttctaag tcagcttctg ctacaagcgt atcaggtgtc acttgctgcc cttctgttac
    22921 tttcacatca aatggcgtac cgcctaagtc aactgtgttg attcccatat gaagcaagat
    22981 ttctaaacca tttggcgttg tgatcccaat agcgtgtttc gttgggaaga cagagctgac
    23041 taccccagta actggagaat agatatgtcc gttttctggt tctacaaagt agccttcacc
    23101 catcatcttt tgtgcaaaaa ctggatcact tgctttatct aaagaaacaa ctgttccgtc
    23161 agcaactgat gagaggatag tcacactttc agctgaagtt tgaggtacta ctttttcagg
    23221 cacttcattt actgtggctg tttcagtcgc taattcttct ttttctaaac ggtctttagg
    23281 aataccgaag aagaatgtag caacgaagcc accagcataa gctgccaata atccaaggat
    23341 gtagcctaac catttaccgt cagagatcaa tgggatcaaa gcggctcctg aaggtccaat
    23401 agcgatagcc ccgacatttc cgatcattcc gatcactgcc ccgccgatac ctccaccgat
    23461 acaagcagta ataaatgggc gtcctagagg taatgttact ccgtaaatca atggttcgcc
    23521 gattcctagg atcccgactg gtaaagctcc tttgatcatt tctgttaatt ctttatcttt
    23581 tttgcagcga atccataatg ccaatgctgc acccacttgt cctgcaccag ccatcgcaag
    23641 aattggaagc aatagcgtac gaccagtttc atcaatcatt tgcacatgga ttggagtcaa
    23701 aatttgatgc agtccgaaca taaccatcgg caagaataat gtgcctagaa cgaagccggc
    23761 aaatgctcca ccaacgccaa gtacccagtt gatcccaccg accatgccat cagaaatgaa
    23821 acctgctaaa ggcatgatga agaaaatttc aatcagaccg atggcaatca atgaaagagt
    23881 tggtgtaacg atgatatcta ccgcatcagg tactacttta tgcagttttt tctcaacgat
    23941 tgacaatagc caaacagcga agatgacacc taggatccca ccttgtccag ctgcaagagc
    24001 ttcaccagta aataagtttt tcagtggtgc ttccggattc atccctgtca gcatcacaac
    24061 ggccccgata actccaccaa gagttggtgt tgcaccaaaa acttgtgcag cattgatacc
    24121 tgtatagata actaggtagc taaacatccc gtttttcaag atgttcatca catcgatata
    24181 ttgttgccat gttgctgcat caagtgttcc ggctgtgact aagtttgcta aaactgcggc
    24241 aatccctgcc acgatcccag taccgacaaa tgcaggaatc attggcacaa agatattaga
    24301 aatgtctttt aataccgatt tgattttaga tggtttttgt ttcgattttt gtgccgcttt
    24361 catttcttgt gcccgttcgt ttacacggtc ttttccagat gctgatgaat ttgctgtagc
    24421 tgtacttaca gaagaaagtt tttctcctaa tttcacgcct gccaaatcaa ccatttgctg
    24481 tgctactttg ttcactttcc caggtccgat gatgacttga agctgttcat cttctactac
    24541 accaagcact cctggaatcg cttttaattc agagacattt acaagatcat cattgatgat
    24601 cttcatacgc acacgagtca tgcagtggac aatgctttct acattgccca tgccgcctgc
    24661 ccctttataa atttgttttg ccagttcttc caaagtcagt tcgttttttg ccatggtctt
    24721 ttccccctca taaggtccct tttacaaatc cgtttgcttc agctaatttt gcttgagctt
    24781 cctctttatc actatttgtc aaaatcatga cgatagccag cttaacattt tgatctgctt
    24841 cttcaaagta cttttcagct gtttcgtaat cagcttctgt cgcttgcatg atgattcgtt
    24901 ttgagcgttc gattagtttt tcatttgttg gtttgacatc gaccatcaag ttattgaata
    24961 ctttacctat accaatcata ctgatagtag aaatcatatt caagataagt ttttgtgctg
    25021 ttcctgactt caatcgagta gagcctgtca aaaattctgg acctgcatcg acttcgattg
    25081 ggaaatccgc gtgtttactg atttcggctt gtttattgca agaaatcgtt cctgtgacag
    25141 caccgatcga ttttgcgtaa tctaatccac cgatcacata tggtgtacga ccgctagctg
    25201 cgataccaat aactatatca ttttttgtca aattcagctt gattagatct tcttgcccta
    25261 gctcataaga atcttcggcc ccttcgacag ctacagtcat tgcttcttct ccaccagcaa
    25321 ttagacctat gaccatttcc ggttctacac cgaatgtcgg tacgcattca gcagcatcta
    25381 acacacctag tcttccactt gttccggctc ccatataaat cagacgtcca ccttttttaa
    25441 aggactcaat cgtgttagca atcactggtt cgatacttgt cagttctttt tcaaccgcag
    25501 cggccacttt ctgatcttct ttgttcatta gttgaacggc ttgagaaata ctcatctcat
    25561 ctaatccaaa agtttcttgg tttctgcgtt ccgttgttag gttctctaaa ttgattttgc
    25621 tcatttttat cttccttctt ttcttataaa ttgatagttc ataccagctt tgatttggtt
    25681 gatcaatggt agctcttctt tgataacttt agctactaca ttcacttttt catccgctgg
    25741 caaatcatat ttggttaatt ggatctcacc catatagcgc aaatactttt cgttatctaa
    25801 agtaatagac cctttcatac gcgtagctgt atttcttgct gggataggcg gaatctctcg
    25861 aaaacgtgca tctacgcttc gtatgacatc tcttgcatcg tcttgccgat tcgtatgctt
    25921 ccctaatata tattcgtaaa attcttcatc tacaggttcc gtatggagca agactgtctg
    25981 ttctttttgg aaagaagcaa attgctcctg aacgttttct gacagacctg catctccgat
    26041 gtaaacaata tccgtgttac ttgctaatag atctagagcg cctgcaagag gatggatccc
    26101 tcgatgttct tccaaagttg gcaatccttg atatagtggg cctctcaact ctgtatctcc
    26161 tggtacaaaa ccttggacaa ggagaccttg aattttgagc cattgatttt ttttctgata
    26221 ccattctttg tccagccctg tttcgggacg tggataataa ttatgccaag cttcaatctg
    26281 tgagaaatca gcttctgctt ctttcaattc atcgatatct ttaggcgtga tcgtgctagc
    26341 attcaaactg attttcaatt gatgtgacca gtcggcaatc tgctgattgg aaatatgata
    26401 atccattctt aaacctgtta caccaattgc tttcaaaggc gttagatctt ctaatgaaaa
    26461 acccgctcgt tccaatgcct ctcctgaaat atcgaccatc aattctagtt tattcttttt
    26521 cgcttcgctt cctagttcga tcaatcgctt ttgatatgcc gaaacatcat cttctggtat
    26581 atgcatggaa gtgaaaatac cgacaaagcc attggatgcc atttggcgga tatactgttt
    26641 cttctcttct gacaaagaat cgttcataaa tattgaaaag ccaaacatat tatccctctt
    26701 tttctgtatc cgtcttcatt tctatgacta gagtatatat ctaaagaaac aaaatttcaa
    26761 tacttttatt aaaataatta aatttcattt ctaaaggttg agtttatatt atcaaaagaa
    26821 ttgtcataac ctaaaaaagg cactgctaag caaaagcagt gccttgaaat attttattta
    26881 aataacacaa aaacataatt actcataaga aaccaattct gtattatcta ccatgaatgt
    26941 aaagaacccc tcattaggca tcggatgaaa ataagcattg atcactctta tcaaggcatt
    27001 atggcaaact aacagataga cattttcttc gtcttcgatg cattctttca ataaaggaac
    27061 gatccgtgca tacacatcta agactgactc cccatttggg aatcgtacag cgaacgctaa
    27121 acgtgctttt tgaaagttct catctttgct tggcagtcca tcataatccc caaaatccat
    27181 ttctaccaat cgttcatcaa cggtcagcgg caagctgaga cgatcagcaa ctgcttgtgc
    27241 tgtatctctt gctcgttgaa gaggggaatg gatgattttc gtgattggaa caggcaaatc
    27301 tactaccttt tctgctagtt tctgtgcctg catctgtcct ttttcagtca gcggcacatc
    27361 tgcccgtccg caaactaatc cttgtgcgtt ccaagttgtt tctccgtgtc tcgttacata
    27421 aagcatgtga ttctctcctt cgttggcaaa gttcccctta accatacctt gttgttcttg
    27481 agattacaag ggttagattc tctcaagaga aataatctca gtagatactt tatccaggaa
    27541 ggtttgatca tgttccacta gtaacatggc aggtttcacg ttcaagatca gttgttctaa
    27601 ttgttcttga ttgaagacat ccaaataatt caatggttca tcccatgtat atagttcagc
    27661 tggctgtgat aaagatttag ccaattccac ttttttccgt tggcccatac tcatctgctc
    27721 gatcttgtta tgaaaaacat ctctttccat cccaagcttt cggaggttgt tcaaaaatgc
    27781 ttggtagtct acttggtttt tctctgcaaa ttccgccaac gttcctcgat tgtcttcata
    27841 attttggctt gcataactaa tgctcagatg ttttggctgg tatttttctc ctataacctt
    27901 gccgttgaat gcccccagaa gataatggat aatggatgac tttcctgcac cgttaggacc
    27961 tgaaatcgcc acccgctgat gaggctcgat tgtaaaatga attggctcga ataacagatt
    28021 ttcataccct aattgaagat cttctacgct taaaagtcgc ttatggtgag acgcttggct
    28081 attcatcgtc aaagaatcga tatattcgat atcttttaat agtttttctt tctcgctgat
    28141 ctgggtctcc atccgattca cgattgcttt ggatcgtttc atcgtccgtg cagcatcagc
    28201 acccactgct cctttattca ctcgtctaga ttcagtatcg atgaatccga cttgtttctt
    28261 tgttttatct ccttctcggg aacgagacca ttcggctttt tcagctgctg ttttctttag
    28321 cctactgact tcttttttca atttttcatt ttgagccatt tcaaattcat cacgaagttt
    28381 tttctgttct tcatagatag agaaattccc ttgataaagt tccagttgac ttttttcgat
    28441 tgctaaaaca tggtccacta cttcatcgat aaatccccgg tcatggctga ccacgatgaa
    28501 gccttgtttt ttctttttca aataagccgc tacttgtttt ctaccagaga tatccaaatg
    28561 attcgttggt tcatcgatta acgggaaatg agtgtcatcc acaaataaaa gtgccagtag
    28621 gacttttgtc ttctctcccc ccgatagtgt gctgaattct ctccataaga tttcaggatc
    28681 tgtttgcatc aattggagct ctctttcgat ttcccatatc tcaaaatccg taatatcatt
    28741 taacacgtaa taggttaaac gttctttatc ttttgtctgt tgcgggaaat aggcaaattc
    28801 ttgctgatgg attacttgcc cttgataagg tagtttgttt tgtagaatat tcagtaaagt
    28861 tgtctttcct cgaccgtttc gtccgataag tcctagtttc cattgtgtgt caaaatttag
    28921 atttgcttgt tcaaataata atgtgccttg gctgtcgtag ccgaatgtca gattttttat
    28981 ttcgatttta gacattttcc atcacctcgt aaaaaaagac cagtcctatt ttcagaactg
    29041 gctaaagagg atgcaagggt tggatacaca caaaaagacc catacatcga aaaaaccaag
    29101 ccaatcccga aaatctgcac actgcttcct tgtctccaca aggtacagtc tgaatgcagc
    29161 tttcatcaat cgacttaatt tttcaatgca cggatccctc actttgtcct atatttgaaa
    29221 ttacgatagc ataaagaaaa tgattttgtc aactacttcg ggaggatggt gaaaaatctg
    29281 ctaagtgtct gtcctaagat ttttgttttt tctaatgttt gcctgtgttc cattgcttct
    29341 tctaaaccta ttggacccaa atgaatactt aagacaattg ggagaaaatc agaaagtcct
    29401 tctagctctt ggtctctact tccgcaaata gcgattattg gttttcgata tttttttgca
    29461 agcactgcta caccgtatgg aacttttccg tggatcgttt gctgatcgat ccgcccttct
    29521 cctgtaatga tgacatctgc ttgttcgatc tttttttcca atccagtgat ttggctgact
    29581 aaatcgaaac cagaaaccat cttcgctccc aacaaaaaaa gggctccacc aataccgcct
    29641 gctgctccac ttcctggaat atcatctaaa tccacctccc agaaatcttt tatttgctga
    29701 gcaatctttc ctgcttgttt atctaagaaa gctagcgtcg tatctattcc tcctttttgt
    29761 cttccaaatt gccaagcagc tccttgttga cctgtatatg gattggtaac atctgcagcg
    29821 ataatcaatt ttactttaga aacaagaacg tttcttcgat caaaatgaag ttttttagta
    29881 gataataaag gatttccttc tgtgtaccct tctaaagaag cacccaagga ttggagtaat
    29941 cctagaccac catcgctaca cccgcttcct cctaaagtga caataatttg ttctacttct
    30001 tgaagcagtg catctttaat aagttctcct aaggcataac tggaagcaag tcggatagtt
    30061 tgatccgttg gtttatcaag tagatctaac cccaatgctt gtgcagattc gatgactgcg
    30121 gtgttttttc cttgccaagt tgtcaataaa tagctgattt ttcgctgacg gaataagaga
    30181 tccatcccat caatggtacg ccatgttccc ctgagcgcgt aatagattgc ttctgcagta
    30241 ttttctccgc catctgcaat cggcacattg atttttcttc ctttacgatc ttcccagcca
    30301 tttagaactg cctgatttag ttcaaatgaa gaagcacttc ctttaaatga atcaattgcc
    30361 gccaaaatat ccatctgtat ccctcacttt cttcttaagt aagacacttt ttttcatttt
    30421 tttcaaacaa aacaatgcta ttttcttcca tttttctata tactaaaagg gaactcattg
    30481 aataaaggag agaaaaaaca tgaaaatcgc aatagctggg gccggagcaa tgggcagccg
    30541 aatcggattg atgctacatc aaagcggaaa tgaggtcctc ttgatcgatc gttggcctgc
    30601 tcacattgaa gccattcgta caaatgggtt gattgctgac tttaacggta aagaagtggt
    30661 cgcaaaactg ccaatctatt caccagaaga aatcatcgaa agcaacgaac acgtggactt
    30721 gatcgtagca ttaacaaaag ccaatcaatt agatgatatg ttttgttcga tccaatccat
    30781 tattactgac aatacttatg ttttgtgttt actaaatgga ttaggtcatg aagatgtact
    30841 ggaaaaatat gtgccaaaga aaaatatctt atttgggatc acgatgtgga cagctggtct
    30901 agctggtccc ggaaaagtca cattgttagg tgatggagaa atcgaactag agaacttgga
    30961 accagaaggc gaagcattta caaaaaaagt tgtagaagtc tttcaagaag caaatttaaa
    31021 tccgatctat agccataacg ttcgttattc catttggcga aaagcttgtg taaatggaac
    31081 attgaatggt ttatgcacga ttttggattg taatattgct gaactaggcg cgcaaaaagc
    31141 ggcagagagc atggttcgaa caatcgtatc tgaatttgct tctatcgcag caaaagaagg
    31201 aatcatacta gatcaagaag aagtgtacca acatattgct tctacttatg atcctgacaa
    31261 catcgggttg cattacccat ctatgtacca agacctgatc aaaaaccatc gtttgactga
    31321 aatcgactac atcaatggtg ccatttggcg aaaaggacaa aaatatgata ttgctacacc
    31381 atattgtgcc tttttgaccc aattagtcca tgcaaaagaa ggaatccttg gcgcagaata
    31441 aaaggctctc tgatgagaat cagctgaatc agcagctggt tctctttttt taagcccaat
    31501 aaaagatcaa aaagaaaaca caactctcat gatgccataa gaattgtgct tgtttgtttc
    31561 agtacataat ctcttcaaaa tgtaggtttt ttatttgttt tcaacagagg agaggtcata
    31621 ttcgtattgc tcataaatag tggaatcgtt agtgataaat cgttttgccc aattttctgc
    31681 atcataaaat tccaatagct ctgataggat tggcttaaat ggtgtatgca gaaattccgg
    31741 taattcatct aaatcacgtt ctcgaagcaa aaaaatattt tcaggacgat aaaaatgata
    31801 atgagcaatc gcttgattat ccgtcactag tttcttttga aaatacatgg attcaaatgt
    31861 ccgcaaagtc actccttgct gaccctcccg caacagttcc agaatcccgc gcgattcccc
    31921 tgtttttttc aggtactctt cataagtaag atatccaggt tgttcattcc ctcgatgtgg
    31981 aagaatgaaa tagttggtcg taacatctct ttttataaat tcctgcttat agctttctgc
    32041 tcgttctttt ctcccattat tcgttgcacc aaaaaataga tccgtctttg gttcgctaga
    32101 agaaaaattc atatgtttcg aataaaatga actattatgt ttcagcccaa gttcttttgc
    32161 ttccaaaaaa tcaaaatgat aaatctcatc gattgccggt tcatcttgtg cctgcttcag
    32221 catcgcctcg tgttctgctt tgaagtgatt ccaaaaaaat aacacaattt ttgcattggt
    32281 aaatgggcgc acgcgtcgag ccatctccat atcaaatcca taatctaaaa aaacgatcag
    32341 ctgataattt gccaactgtt gtctagctga tttaatgaat tccgactgta gtaaatcttt
    32401 tccggaccaa acatctaaag agcggatgga aggcaaaaaa taaagtgatt tattttttgc
    32461 aactagcagt acgtcatttt tgttcatctt ttccgcctca ctttctctgt cactttttcc
    32521 tatgatagcc tgtttaaata gtaaaaagcc agtaaaacgt ttcctatatt tgtatgaaaa
    32581 tttaacattt gtatgaaaat atgatgaaat aaatcattct tctattaaaa ttcgcctaaa
    32641 agttttctga atagttgtaa ttgtctgaaa atttgttatc attagtcatt gttaaatatt
    32701 gctatttatt cttaatataa attttgattg tgaatttaaa agaaagaggt catttatgga
    32761 agataaaaaa ttgaaaaaac aacttagctc cagacatatc accatgttag ctttaggtgg
    32821 agcaatcggt gcaggtctat ttaaaggaag tggcgaagcg attgggatcg ccggcccttc
    32881 tgtattgatt gcttttttga ttggtgggct gattttatat atcgtgatga aaggtctggg
    32941 aaaaatcgtt ttaagcggtg gcgacacgca tcacggatta tctggtttga tacgccctta
    33001 tctaggtgct cattctgctg attttactga ttgggtctac tggtctatgt ggattatcaa
    33061 tatcatcgct gaagctgttg cagctgcttc ctttttgcag ttgtggttcc cgcacattcc
    33121 agcctgggta tttgttttta tgttggctgt tttaactacg atcatcaatc tttattcggt
    33181 acggttattc gcagaaacag aatattggct ggcttttgct aaaatcagtg tcattatctt
    33241 actgatcatt tttgctactt atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc
    33301 tacccttcag caattgactg accatggtgg gtttactcct catggtatga agggaatcat
    33361 cagttctttg cttgtagtta tctattctta cggcggttct gaactgattg ctatcacggt
    33421 aagcgaagca gatgatccta aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag
    33481 aatcatttcc ttttacatca ttcctttgtt tttgcttttg attctttttc cttggaatac
    33541 tttagcaggc acgaatgtca gtccttttgt gatggtattt gaaaaaatga atatcccatt
    33601 tgcagcggat atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta tcaattcggg
    33661 tgtgtatgca tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag gttcctctag
    33721 aaaactagct gttttaaaca agcatcaagt accgcaacgt gctgttttat tttgtaccag
    33781 tgttctttat cttggtgtag ctctttctta ttttgtcggt gacaaattat ttggctatct
    33841 ggcaggttcc ctttcgtata cagtattatt gatctggatc attatcagtg cggcatcctt
    33901 tgtactggca ctaagaaaag gaacattatg ggataaagga atcagctttt ttgcattagc
    33961 tgtcttagga ttgattttcc ttggtatcct gttcacgaac tccattgggg taactgtatt
    34021 gactggcctg ctttacctgt tcatttattt cagttatcaa aaaaagaatg atgcgttcgt
    34081 attgacgaat gaatgaaatc gatagaaaat tttattttat ttatgtggaa tagatggagc
    34141 tattgaagaa aggagttgtg acaaaagtct tgttgcaact cctttttccg aataaacagt
    34201 ctcatgacct cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat tgattgttct
    34261 ccatgctcat gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa
    34321 agtattggaa gtttttgttg atgatcggtg tttcaacggt agttatgttt ggtatgatgt
    34381 acttgaatgt ctatgcgctc gatcatatct tcttcagtcg tacacgagta ttcatggcgt
    34441 tgatgatggg tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa
    34501 acaaaaagat aaatgttatg atcttagccg ctagtcttct tgtttttgcc ggttccttgt
    34561 ttatggtacg aagccaaact actgtcaacg acacagcttg gatgaaggcc atgattcctc
    34621 atcattctat cgctatcctg gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa
    34681 aattagcgga agaaatcatt caggcacaag aagaagagat cgacaaaatg aaaaaaatga
    34741 tcaaagaaat ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta
    34801 tcagcttcaa gaaataagaa gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa
    34861 aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagcttctc ctatttttcg
    34921 tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttatta agaggttgta
    34981 aaatcagcgt tctgacctga gtattagaga gacaaatgga aaaatagttc ctcatatttt
    35041 gccatttttt gagctaatac cgagggtcag ctgattgaac accgtttatc gagaggttgt
    35101 gaaaaagtcg cttagtttcg cgtattaaga aaaattttgt gaaatggctt tccacatttt
    35161 tgtataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg
    35221 gattgtgaca aaagtcttgt cacaatccct ttttatgttt ttattgttct aattgcgctg
    35281 tcttgcttac tgcttcttgc ggtttcacaa atttttttga gattccccaa cctgtgaagg
    35341 cactgaccat cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc aagtaagtga
    35401 gtgtaggaat accaaatgta cttgctacga aacttccggc aattccccaa ggcactaagt
    35461 agttgattac tgttccacca tcttctagcg ttctacctaa tacagttgga tcaaatcctg
    35521 ctttttcata agtttcttta aatgcattac caggcagaat aacagatagg aattgttcac
    35581 caataaaaat atttactccg atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt
    35641 tcgtcatctt tcttgatact cgttccatca cggttgagat caagccaaat tcgatcaaca
    35701 atccacctaa tgacaaagtt agaataatca atgacacagt aggcatcata ctttcgattc
    35761 ctccgcggct aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca aaccctgatt
    35821 cgatgacccc agccaacttc gttaaggaag tttgtgggtt ttgaataaaa atcattccta
    35881 ctgctacagc gatattcaac aaaatcgtta aaattgccgg cacctttttc catgcacaca
    35941 gtaacattaa aaataaagga atcagtgacc agattgaaat agtaaagttt gcttctagaa
    36001 catcagtcac ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca
    36061 tgaataaaaa gaaagaaaca acaaatgccg ggacagttga ccacatcata tgcttgatgt
    36121 gtttgaacaa atcggcatca acaatagctg ctgctagatt tgttgattcg gataatggag
    36181 acatcttatc tccaaaaacg gcacctgaaa caatagctcc aacaaccagc gctggattta
    36241 agcctagtgt tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg
    36301 cactccctac agcgctacct acgattgcac acacaacaaa tacagaaggg acaaaccact
    36361 ggacactgat caatttgaac ccaatgacca ttagagttgg aatgatccct gcttgtatcc
    36421 atacagcaat caatgaaccg actaaaataa aaataaagat tgggatgatt cctggtttga
    36481 tgccattgat gattccttcg tgcagccaat ctgtcgggta tctttttatc actgcataca
    36541 acatgaccat tccgatcgct gctaagatcg tgatattagg tgagaccttt aatccgacaa
    36601 cgccaatcgc aatgcctaaa acaataacca ttaatacaag tgttgcttct aaaaaacttg
    36661 gtgttccttt ttttcccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa
    36721 gttaagatat cggattactc cgccgcaatt catccgcgat tcctccttta taaaaataaa
    36781 aagacctgtt ttaaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat
    36841 ctgtttttca ctttcaaact tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt
    36901 aggcttgatc ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccgctactgc
    36961 gtgaaaagtt gacttgtacg aagtttacct tccataagat catttgtcaa tgaaaaagaa
    37021 aatatttttt catttttctg aaaattattt ttataacttt cacacacaag attatttttt
    37081 actagaaagt agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgcttga
    37141 ttctttctaa tagctttggc tctttcaaca gatcaagagc tgtattggcg agtagttctg
    37201 ctgcaatcgc aatagaagca aggcctttgt cacttttagc agcagctttg aattcttctg
    37261 tgtgtccggc aatatatgtc tcagagattg ctaccgtagg ctggattgtt ggtatgactt
    37321 ggctgacatt tcctacatca gaagaaccga gacttgttct ttgttccgtt tctatttctt
    37381 catctggaac acctgcttct tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa
    37441 cgatgacatc atcaactgca ttttggaaca atccaaactc aaaagtcgtg cccgtactta
    37501 tcgcagcacc tcgtacgata ttttccactt tttcatatac tttatctaat gtttgccgat
    37561 ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg aatgacgttg gcagctactc
    37621 ctccgtctgt aatgatccca tggatattga catcttttgg caattgcaag cgtagagcgt
    37681 tgattccatt aaacacttgg atcaatgctt ccaaagcatt tactcctttt tcaggtgctg
    37741 ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt
    37801 ctgtcgtttt cccataacga aaagctggat ggacgcataa cgctacatcg acatcctcaa
    37861 aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct tcttcccctg
    37921 gggtgccata tacacggatt tctccaccag tttcatcaat gaattgcttc actatgctgg
    37981 cggctaatac ggagtaagta ccaaacaaat tatgcccgca tgcatgacca atacctggta
    38041 aagcatcgaa ttctgccaaa aaggcaattg tgggaccggg tttattggaa atataacgtg
    38101 catcaaaggc tgttcggtgt ccagctactt ctttttctac ccaaaacctt tctttttcta
    38161 attgtttaat caacgctgct gaggaataaa actcatagtt acttacttca ggatgttcgt
    38221 ggatatctag agcaagtgct tgatattctg gtaaacgata ttggattaga tccttcaatt
    38281 ggtttgttga gattgttgtt gtcataaaaa attcctccta tgctagtcat ttttcttatt
    38341 ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt tgatgttttc tcgattactt
    38401 tgactgtgtc ttctgtttga taagcgtcta ctattttttg atacgtttta ttgttttcat
    38461 cttcttttct tgcaacgatg atattcacat aaggacgtgc atggtcatcc actggttcta
    38521 agaagatggc atcagaagca ggattaaatc cagcatctac agctacaccg ctattgatca
    38581 ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga agcatctaat tcagaaatgt
    38641 ctagattttt tctattctcg gtaatatcag aaacagtggg cgcttgtcct tttgccttat
    38701 ccactttgat caatccagca gtttgcaata acagaagagc tctgccgcca tttgtaacat
    38761 cattagggat tgctactttt gctccgtctt tgatatcagc tacatccttc aaagtgttag
    38821 aataaattcc cagcggtgca ttgacagtat ttccgatcga tactaaatca gttccgtgtt
    38881 ctttattgta attgtctaaa aagaattgat gttggaaaga attcaaatca atcgatccat
    38941 ctgctaaagc cacatttggt tgattgtaat ctgtaaattt cacgtattcc aaattaatgt
    39001 tctccttttt caaacggtct ttcacgttgt cccatacatc tgtatcttct ccaataatcc
    39061 ctagttttac gatagtttct tttttgtcag ttgtatctgc ttttccttgg ctacaccctg
    39121 ccaaccaaat aattgctaca aatgtaatga ctacaaaacg aataattttt ttcatttttt
    39181 ctcctccact ttcaatgtgt aattttttta atcagttgat tactgataaa ttgacttaaa
    39241 aaaactaaga ttagaatcaa taatgtagcc acaatcgtta catctgtttg gaatcgatta
    39301 taccctctcg atatagcaag attccctaat ccgcctgcac ctactgctcc agccatggta
    39361 gtcagtccga tcacgttgat gatagtcaac gccgagaccc gaatgatacc aggcaaacct
    39421 tctaacaaat acacacgtaa aatgataccg attggactgg ttcccatcgc ttctgccgct
    39481 tcgatcaccc caggatcaac ttctagcagt gcgttctcga tttgacgagc aaaaaacgga
    39541 acaactccaa aaatcaatgg cactaaagct gctttcgcac caatcgtcgt tccaactaag
    39601 aaacgagtag tcaatgctaa aagagaaagc aagataatga aagggatgga acgaatgata
    39661 ttgatcagct tatcaagtat ttcaaatagc agacggttag ctagtaatcc attagggcgt
    39721 gtcactacta acgtcacacc taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa
    39781 aaagacatat atagtgtttg cttcgtcgct tcgacaaatt catccggaat caaataaaca
    39841 ttgggcaaat atgttttaaa gaaatcagtc attttatctt cctccttcga tcactttgat
    39901 acgactatct aaatcgtttt tttgtaaaat atttattttt acaccagatt tgatcaaata
    39961 atccaatgct tttttccgct gtttggtttc tccagctaaa gtaacgatca aactccctaa
    40021 cggcgtttct tgtaataact ctacattgcc atataaaata ttcgctgtta cttgatattt
    40081 gctgtacaga tgagcgatta gaggttcgtt cgtctgattt ccaatataac ttaattccac
    40141 cagccattta ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc
    40201 ttggtcaaga tgagtagctg ttcgaataaa atctttggtc aattcttctt caggattgct
    40261 gaagatctgg atgctgctac ctttttcaac gattcttcca tcttccataa ccgctacctt
    40321 gttgcagatt tctttgacaa cttgcatctc atgagtgatc aggacgattg ttaaacctaa
    40381 ctggcgattc aattttttta acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc
    40441 actggttgct tcatcacata ataatacctt aggttcattc gccaaagctc gtgcgattgc
    40501 tactcgttgt ttttgtcccc ccgacagttg tttaggataa gcagatgctt tttcttccaa
    40561 accgacaagt tccagtagtt cattcacttt ttgacgacgt tcctgtttcg attttccaga
    40621 atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta gattgaaatg
    40681 ctgaaagatc ataccgatgg cctttctttc ttttcgcaat tctttgggag agagctgact
    40741 cagattcgtc tgattgacaa tgacttctcc tgctgttggt ttctgcagca aattcatcac
    40801 acgaactaac gtacttttcc ctgcacctga gtaaccgacg attccataaa catcgccttt
    40861 ttcaatcgtt aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca
    40921 tacagaaaca tctcgcaatt caatcattaa aattcctcct tatctaaaaa aagcgtcctc
    40981 aagaaaatga aaatgtcatt ttcttgagga cgcttttttg cgtgttacca cctcatgttc
    41041 aagctgtgtt cacacacaac tcctcttcaa gtacgatcat actctagcaa gatatcggtc
    41101 gcagccgtca tcgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat
    41161 agtcgttcct tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac
    41221 ttaccatttc aatgcaaata atattcaaaa aaaaagtcct tttactaaca taaactatgt
    41281 cagtaaaagg acgacgtatc gcggtaccac cttttttcgg aagtttcctt ccctcaagcc
    41341 caacatctca aaaagagaga ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc
    41401 tacttatcga caaattattc tccaaggcca ttttcagctt ttcttacttg actcttctca
    41461 ccagccagag cctctctgca aagtttccaa acttactttc cttttcaacg aaatcgatat
    41521 tttgttgttg ggattaatct acatgaggaa aactgaaaag tcaagtacga aattcaaatt
    41581 attttttcta tatttatttt tctgtttttt tatttaacat ttcaataaca gttcgaagat
    41641 cttttccttc cgcaagaagt ttcaattttt ccttttcttc gacggtataa tttcggacaa
    41701 aactggaatc aggtatcaaa acaacgccta atcctgctcg tgaagcagct gttgctccta
    41761 ctaaagaatc ttccactgca aaggctgaac tagcttctaa tcctgttcgt tccaaagctg
    41821 tcagataagg aatcggtgaa ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt
    41881 ccaagtaatc agctaattta aagtgatcca agatatctgt tgctcttttc gtaacagtgg
    41941 tcgtagcaat gcctagtttc atatgacgtt cttttaagtc tgccaatatt tcttttgcat
    42001 atggttttaa gtggatggct ccgcttgtca gctgttgata aaaatattct tcacgcttcg
    42061 ctcggacttc ctgtactttt tcagggctac ctgctgcttt cactaaaata tcaaaacttt
    42121 gtttccagtt ttttccgccc caagcgtcaa cgatttcttt tgggatatgg atatcaaatt
    42181 gctgaaatcc ccaaagccag ccatcacgat acgttttttc tgtgtcaact aacaaaccat
    42241 ctaaatcaaa tattgcacct ttcatctata cattaatcct ttccaaaaca atacacatag
    42301 tttagcacga ctggtccaaa aagaacagaa gcaaagaacc agccttctcg tttataagag
    42361 ttcttggaat cgacggatat agattttctt cacagctgtc acaagcgcca gataagcgat
    42421 gactgtagca aataaccata accagtaatt gccaggaagt ggcatcaagc ctaattcagc
    42481 accaaaactt gtaaaaggaa ggacagaacc gacaataatt cctaatgtag tgataacaaa
    42541 catggcaaat gttgcgttgc tttggataaa tggtactttc ggtgtacgca gtgcatggag
    42601 cactaaggtt tgtgtccata gtgattcgac aaaccatcct gcatggaata atgcgatgaa
    42661 aatcactcgc tgtgaactat ccaatgtatg gaaacttccg cccactacag caggacaaat
    42721 aacaaaatac atcaacaagt aagtagtaat atcaaatatc gaacttgttg gaccaaagta
    42781 aaccatgaat tccccaatac tagatgcatt ccattttttt ggctcgtcca aatattcttt
    42841 atccatccga tcccatggca aggaaatgca ggaaacatca tagatcagat tcaaaaacaa
    42901 aagttgtaaa ggcagcattg gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa
    42961 catattgcca aaattagaac ttgctgtagc cttgatatat ttcatgatat tcccaaaagt
    43021 ttctcgtcca gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta aaaggatcac
    43081 gtctgcagat tctttcgcaa tatccacagc tgtatctaca gaaataccga tatcagccac
    43141 tttcattgca ggagcgtcat tgataccatc tcctaaaaat ccgacagtat gaccattttt
    43201 acgtaaagct gtcgtcaaac gtgctttttg ctgaggattc aattttacga atacttcatg
    43261 gtcttcagct acttgtgcta attcatgatc actcatttcc gagattttct caccggtaac
    43321 caattcattg atctctagac ctacttgacg gcacactgaa cgtgtaacta attcgttgtc
    43381 cccagtcaga acttttacag ctacaccatg ttctttcaga gctttcaacg cttgtttcgt
    43441 tgtttctttt ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc
    43501 tttgactgaa aattctccaa cgacgcttgg attggtcttt tgtgcgacac caatcactcg
    43561 taagccatct tcattaagtg actgtacttt tgcaagtacg gcttcttttc gttcttttgt
    43621 taatggtgta acagtaccgc caagatcaat aaatttagaa atgttcagca tttcttcaat
    43681 cgcacctttt gtgatcattt gtgttttacc agctggatca gcaaccacaa cactcatacg
    43741 acgacgttca aaatcaaaag gaatttcatc cactttttga taattgatct tatctgtatc
    43801 taaagtttct gttgcttcat caatgatagc cacatctaac aaatttttca gccctgtttg
    43861 atagtagcta tttagatacg catggcgcaa aacacgatca tcttcatttc cttcacaatc
    43921 catatgatat tcaagaatga ttttatcttg tgtcaatgtc cccgttttat ccgtacataa
    43981 cacatcgatt gcaccaaagt tctggatggc attcaaattt ttgatcactg ttcctttttt
    44041 ggccatcaca cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg
    44101 tgttagacca actgctacag atagtccaaa aagaaaagct tctagccaat ccccttttgt
    44161 aagcccattg atgacgataa ctgctggagc catcaatgac ataaatttga tcagtagcat
    44221 ggatgtttta tggatgccta attcaaagct tgattgaatt ggtttttcag atagcgtttc
    44281 tgctacatga ccaaatagtg tttcatttcc aacagaaaca atgattcctt ccgcacttcc
    44341 actgatgaca ttacttccca taaagacaag attttcgtaa cttgtttcgc tttgctgatc
    44401 gtcttcacat actttcgcat gcttttctac tggatagctt tcccctgtca atgctgcttg
    44461 ggaaatgaat aaatcctttg cggacaataa acgcatatcc gctggtatca tatcccctgc
    44521 tgaaagccga acaagatctc cacagacgat ttcttccata ggaatctctg tgtactttcc
    44581 attacgtttt acagctgccg ttactttcac aagtgatttc agtttttcag ccgcttgatt
    44641 ggaacgtacg gactgaatca acgtcattgt cccgctgacg ataaccatag tgaagataat
    44701 ggctacaccg aaaagatctt tatctccagg agctgctaat acatattccg tgatgaatga
    44761 aataattcct aatgcgatca acactaatgt aaatggcgtg atgtatgctt tcaatatttc
    44821 tacaatgaat ggtgtttttt tgccatatga aatcgcattt tcaccgtaat tttctcttga
    44881 acgttcgatt tgttcttctg tcaatccatt tgctgatgca tggaatagtt caaataattc
    44941 ttgtttcgtt tttccagcaa aagctgcata gattttttgt gtatctttct ttttttccat
    45001 ctcaatctct cctttgtgtc caaaagacag ctgttctcgc tgcttttggt ctttagctat
    45061 tcatcggatc gatagagaaa aaactgaaag gggctgagaa cagatagata atagccctgg
    45121 cctttcctct agatccttgt ttttcgaatg actgtcatcc atttgttctc atctccttta
    45181 ctaagtaatt ttaattcatg acttccaatc atttttcgaa tagttatttt tccaattaga
    45241 ccacctcctt tttatttttc accgatgggg atttgcggca acaaaaaaac ctctcgccaa
    45301 atactcagcg aaaggtttga aaaagcatat taaaaaaagc ttctcagatc tcatcgttga
    45361 gttttgacac tgtacaacgt aaaaagcaga tacctgcttt tagctacctt atgaaaagcc
    45421 ttatctcgac aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct
    45481 atgtttgtat aggagcctca cctaacaaat cttattcggt tttccttcgt taatataaca
    45541 actaaaaaag aggatgtcaa atcaattttc catgtttttc atactttttt taaaatacac
    45601 acgttatatt cctgtctcac gcggttttat ttgtctcatt ttccacctcc cgctataata
    45661 gaaatatagt aggaaacgag gtgctgttat ggaaagaaca cttgttatta tcaagccaga
    45721 cggcgtgaga cgccatttag tcggaagcat catccaacgt tttgaagcaa agggactggc
    45781 cattgctgaa atgaagtttg aaactatgac tcctgaatta gcaaaagaac attatcagca
    45841 cttgactgag cgcagttttt ttgatgaact gatcgattac atgacttccg gtcctgtcgt
    45901 ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac
    45961 aaaagctgcc gatgctgttc caggtacgat ccgaggagac tacgcgctgc cggggactga
    46021 aaacatcatt cacgcttctg attcccgtga tgctgcggta aaagagatcg cccgcttttt
    46081 cccgccattc gatataaaca aaccgtctga aaaagcggcg aagtgataca aattctaaac
    46141 tgctggtcta gttgctgggt tcagcaatgg ccagcagttt tttctctaag gacctccgtt
    46201 gattctttcc taacatatat tcgagcactg tgaagaattc ttggtagtct ttcgtctttt
    46261 cttcccctaa agcagcagaa atcaatcttt gataataatc tgccatcaat gtttcataga
    46321 tcactgtact tctttcttta tgtgctaagc tccgtttcat atcttcattc acactacgtg
    46381 cctttttcaa ccgaccattt tctatataaa acgaagtcag gttcattaaa aaagtaaaga
    46441 gatactgctc atgattgttc aactgtgcta atcgtcgcat tctttttttg attttcaaat
    46501 aattgttctc aatgaaccga tcactgaata tccaaggaaa atcagctaga atagatagtt
    46561 caaattgacc atagtctggt atctgcatca agtattcttc aatgattcgg cattcataac
    46621 gtaaaaattc aaattctgtc tcagatactt ctgttttttc gaccatgtga agattcaaaa
    46681 ccaaataata cagatcttct gttttcttaa aatgcctacg gttattgctg atctctttat
    46741 aaggatcaaa gctgtcttct tgtacccgat ctagcattcg tctttttttc tttaacttta
    46801 ttggttcatc cagctggttt cgcatccatt catattctgc aagtgtcatg ttcattcgct
    46861 ctaaaaatga tctcaatgtt acaaatgaca tttttttcac tgaatttgtc tcaatcccga
    46921 ctagagaagt cttaaaacag attccttccg ctaattggga ctgtgtcaac ttcttgttga
    46981 ctcgaagttt cttgatcaat tcaccatcat acataatagt tctccttttt aatttaaatt
    47041 tttccacatt caataaatag atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa
    47101 tgtttttttc ataaaaaata ccaaccttca agcattagaa tgttgtctaa ctactgaagg
    47161 ttggtttgat tgtttattga ttattttttt gctgttcaac gtctgttctt gttactggat
    47221 ataagttggt tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt
    47281 tttgcttttc gattgcttca ttacgttccg cttcattttt atagtcatat tttttcggat
    47341 caatcgtctc aaatccgttt ggcacataaa accgcaagag attttcctga ttcagcttat
    47401 ctgaaagact taacgcagtc ttgacttttt cctgatcttt ggagatttcc tcatcgattc
    47461 ctgctgcttt cgtatcgatg atttctcctg tctgattatt gtaagctttt cctcctagga
    47521 cggtgtattt gggtgtcaca aagttcccat ttcgaaaagc aactacttga tcatgctgag
    47581 aagataataa gtctgtacca aaatgaagat aatctttatc atcgattccc aatagatgta
    47641 gtaatgtcgg caaaacatcg atttctccac cgtattcatg attgactgtc cctttagtat
    47701 aaccaggcat atggatcatc aatggcactc gttgcatctg tgcgttgtca aattcatccc
    47761 atgtatctgg atctttacca agagcgcttg ctaaatcttt attatctgta ttcgagatcc
    47821 caaaatgatc cccgtaaatc acaaagattg aattttggta aataccagat tttttcaaat
    47881 acgtaaaaaa ttgttccaat gattgatcca gataatgtgc cgttcgaaca taattatcta
    47941 ctgtgctatt gccagtattc aaagatggaa agtcgaaatt cttgtcatcc gtataataag
    48001 gcgtgtggtt ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat
    48061 attgggcaga ttctctgaat aaatctttat ctaatatccc ataacctaac gtttcgtcgg
    48121 attcatcaaa ataagatcga tcaaagaaat tgtcataacc taagttttta tatacatgat
    48181 ctctattcca aaaactagct acattcccat ggaaaacagc gcttgtatag ccttgctttt
    48241 gtccaaggat cgctggtgcg gcttgaaaga cattgtctga cccaagttct gtgaataatg
    48301 agccttgagg taaaccaaat gtgccagttt ccaacatatt ttcagcatca cttgtctttc
    48361 cctgtcctac ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc tggaaaatac
    48421 tatttaaaaa cggtgtgact tcttgtccgt ctaccttcat attaatcaaa aactgctgga
    48481 aactttctaa atggagaacg atcacatttt tccctttagc gatcccaaat gtttcgggat
    48541 ttggctctgc ataatgtttc tttgtataat ctaaaacttc atcaatccca ttactgcttg
    48601 catgcgcgcg aacactgcta gtcattcctg ttttgattcc gtcataaata gtatacgcat
    48661 cgattcctag atatttgaca atatatgaac ggtcaaatgt tcgctgcagc aattgtggac
    48721 ggttggcttc gctcagcgct agatttcctg aaaaaataag ccctgaaaga caggtaacag
    48781 cgatagccat cggtttgccg acagggttgc ttttgatagg tattttcttg accttcaacc
    48841 aaaccagtag cccaatgaac acagcgatat ccagccagta gatgacatca tggggcttca
    48901 tcaaagaaaa ggagcttcct gacaatcctt gcgacacttt cgaaaagcct aatacagatt
    48961 tgactgtaat gaaatctgtg aattcccgat aaaaaataat attcaagtac aacaaaagcg
    49021 tatttaaaat atcaatgatc aaaagaatag ctaacgctgg ctttggcttt ttaatataca
    49081 aagaaaggcc aaaaaacaat aaggtagtcg caattgggtt gatccacaag ataaagtact
    49141 ggatcgtacc ttccacacct aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc
    49201 agaaaaagaa aacagctgtc aaaacgattg ctaaacgatt ttggcacaat tttttaagtt
    49261 gtgtcacgta tatcacttct ctctattcac attttttctt attttgctcc tgaaaaatga
    49321 aaatttccta aaaaagttgt tatcttaaga tttttttatt ctttctaaag attattccgt
    49381 ccacttttca cagcataaaa ccgctgcgat gtaagcgatt aaactaatga agagataaaa
    49441 gtcttgtggt gaccgcttca ctccacggta ttcaaaagcc agtttctcgg tattcgtaca
    49501 aaaaaagagg ctgtgacaaa attcttgtcg cagctcctta tttcttgatg caggacagct
    49561 ttttcacaac ttcttcctta aacggtgttc tcctgccaac tcctcggcca caagtcacaa
    49621 tatttataaa acatgagaag ctgttttttg aaattgttcc ttgtggctca gagttaaacg
    49681 gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccctcggt attagattat
    49741 gtcaggacgc taattttaca acctcttggt aaacggtgtt caaaagcttg catctgcggt
    49801 aataagccca accaaacgaa aaatataagg agctattttt tgaaattgct ccttgtggct
    49861 cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccttcg
    49921 gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac
    49981 tcaggtcaaa acgctgattt cacaacctct gtttttcatt gtccatagga ttgatattgt
    50041 ccttgtgatg actcattcgt aataattgag gcttgatttt cttcacttcc cgttatcgtt
    50101 tgtccactgc cagaatctga aggacttgtt cctgtcgact cgtttttttg agaagagaca
    50161 gttgttttct tgacttgttc atttgtcttt ttccattccg ctaaacgatc ggcgaccact
    50221 tttttgtcta ctttttcaga ataagcggtg atcacttttc catttttcaa acgggctact
    50281 ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa ttgatattgt
    50341 ctgagagaaa tcgcactaag tacgtcatcg atattaataa attgattttc ttcttttcct
    50401 tttggcaaat aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt atactccgcc
    50461 caccaataag ttgagaattc ttttttgaaa ttatctactg attctttgct tgctacgatt
    50521 ttttctgttt ctttctgagc aagatctgct gtcatttttc ctgaaaatgc tgcggataaa
    50581 ataagcagta agaaaaaacc gaataaccaa aagagaagaa acggtctttt tttcctcatg
    50641 ctttcctcca ctcctattta attttgacta ttctattata tacaaaaaat gaaatagaaa
    50701 gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa atggtaagaa
    50761 tggagaattt cacgcatttg ctctcaacta ttctttctta agcctcaaat ccgtttttat
    50821 ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt tctttttcca tcttgtgcta
    50881 agtctactga aataaagcca tatcgattct tatatgcatt ggtccacgac cagttatcca
    50941 tacatgtcca catatgatag cctaaacaat tggcaccttc ttgaatggct tgatggacat
    51001 atttcagatg agattggaca aattcgatcc gataatcatc gtggatcatg ccatcttctt
    51061 tgataaatcg tgcttcgttt tctacaccca taccgttttc agaaataaag cagtcgatat
    51121 ttccataatg atctcgtaca ttgatcaaaa tatcatagat tccttgttca tagatctccc
    51181 atccgcgata aggattcatt ttacgaccag gcatttcata attgtcgaaa taatcctcag
    51241 gcattggacc gtttttagta tcgattggtg tttcttttgc ttttacccgg cgtggctggt
    51301 aataattgat acctaaaaga tctactgtat tttctttgat aaccgtcaaa tcctctgcat
    51361 ggatcacagg catgagatca agttctttta ctatctcaat caattcactt ggaaactcgc
    51421 cttttacagc tggatcaagg aatgaacgat tgaagaacgc atctgctatt tgcgctgctt
    51481 taacatcttc agggtttgat tcgtctcttg gatagcttgg tgtcagattc aagatgatac
    51541 caataacacc accttgctcc atttcatggt aagatttgat ggctaacgct gaagccaacg
    51601 cttcatgata gccaacttgg atcgcttttt tcatatctga ctcttctgga taatgccatc
    51661 gatataaata acctgcttcg actggaacga ttggttcatt atgagtaaac cattttttta
    51721 ctttcgaacc aaacaattca aaacaacttt tggcatattt ttcataggct tcaaccgttt
    51781 ctcggttcaa ccatccgcct ttttcttgca gatacatcgg catatcgaaa tgatacagat
    51841 tcacaaaggg ttcgataccg ttttcaatca actcatcaaa ataacgatga tagaaacgaa
    51901 cggcttcttc gttgattgtt ccagattcat caggtatcaa acggctccat tggatagatg
    51961 ttcgaaatga attatgccca gtctgtttca tcaactgtac atcctcttga tacttcgtat
    52021 aaacttgcga ggttttttca ggtcctactt gatcaaagaa cttttgcggt tcttttttgt
    52081 accaataatc aaaaatgttc tctccttttc catcgcctga aatccttcct tctgtttgtg
    52141 ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt ttcatcaaag
    52201 cacctctttt tcttttttag ttcgttttag ttgattccat gtatgatatt ttccatcaca
    52261 atcacttttt cattttcttc ctcttctttt ttgattcgtt ttaacagctg ttctgctgct
    52321 cgttccccga ttttatctgt tggtggcgtg atcgtgattt tcggaaggat actcagatcg
    52381 tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct gacaccttct
    52441 tgattgaaac attcgatggc accaatcgac atattgtaat tgctgacaaa aatagcagtt
    52501 gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga atagtctcct
    52561 gtaaagacct ctaattggat cgatggatca ttttggaccg cttctttggc tcctgctaaa
    52621 cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa atccgatttt tttatgtcct
    52681 tgttcaccat gatgagcgat cacttttgct acactttctc gatcattcac taatattgta
    52741 tcaatacctg ctggtccttc tggacaattc aatgcgataa ctggaatact catttttgtg
    52801 agcatttcaa atccagacca ctcttcttcg gataagaaga caatcagccc atcgatcaca
    52861 tgcttcatca ggtattcaat tttttgttca attttttctg gctgatcggc aaacccgctt
    52921 aataataagc tatacccatt ttcttcagct actctttcga tccctgagac aacttccgac
    52981 ccaaaacggc tggaaatccc gtttatcaaa agtccaatag agaagcttcg attattcttc
    53041 aaccctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc tgctattttt
    53101 ttcatgttct tttccttcag ttgctgacca ttcaagtaac gggaaactgt tcccactgaa
    53161 acaccagcca tcttagccac atcacggatc gatgccatac attctcactc ctgccagtta
    53221 catttgattt ctctttcttg ttgttcgatt atgatgaccg cattctttgc atagtgttgc
    53281 ttgtcatcaa tatccatata actaaagtct tcagtagagc aacttcctct cactattaat
    53341 tatacttaaa caagtatttg aaccggttca tttttaatat agcatttttt taaaagattg
    53401 caacactttt ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt aaaattttat
    53461 tctaaaattt actctatttg tgggtgtttt gtgcgttttt ctcttccatc cgtttgaagt
    53521 tttacattcc aatggtataa tttctttatc ctatcaaatg ggggaagtat tttgaagaaa
    53581 ttaatcgtac tatttatttt gtctgcggga cttttgacat tgactggctg tgatttgttt
    53641 ggcggctctg accaatcctc ggacgaaaca accgattctt ctgtcttaaa agcggaaaaa
    53701 gcagatacca ccaacagtgt atatacgcta aaagtacgta aagatatgga tttatccgcg
    53761 ggacagttga catttacaga taaaaaaatg gaatggacac gcacgtatca gacacaagga
    53821 actgattctt ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa gatcactacc
    53881 aaagatgacg aatatattat ttcaggaaaa gaaaataaaa aagaaacaac agtcatattt
    53941 acaaaaatcg gtaattaccg tttgaaggat gaggatggca acatctacag tctatagtgc
    54001 cgtctgttgg atcttctttt gtttctcccc ttcttttctg ctatagtaaa gaagcaagat
    54061 gattgtaaag gagacgaaat ggatgatcga tagtcctgat tattgtacat gtaataacaa
    54121 caaaaatatc catacagaat ttaatgacca aggaagcttg agcatatgcg acaactgtca
    54181 caaactagtc aaagattcat ttaaaacatt tgaatcttat cctgttcaaa attatgtaaa
    54241 aagtagaaga agataaacaa atccaagcaa aactcactat ggctgatttt tatgaacttc
    54301 aaactcagct tcttcctttg atcaagcttc atcagtaaaa caaacagtgg atcatttcag
    54361 tttacaaatc aaagagtaaa gatttattta tttcaaatat ttgaatataa aataaccaaa
    54421 cgatagaaag gcttattcta catcacgtag ta
//
LOCUS       DANKBF010000011        54193 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000011 DANKBF010000000
VERSION     DANKBF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(751..1254)
                     /locus_tag="PWY89_000686"
     CDS             complement(751..1254)
                     /locus_tag="PWY89_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2065744.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1613..2638
                     /locus_tag="PWY89_000687"
     CDS             1613..2638
                     /locus_tag="PWY89_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2065745.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2961..3839
                     /locus_tag="PWY89_000688"
     CDS             2961..3839
                     /locus_tag="PWY89_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL2065746.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4315..4431
                     /locus_tag="PWY89_000689"
     CDS             4315..4431
                     /locus_tag="PWY89_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2065747.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5359..5697
                     /locus_tag="PWY89_000690"
     CDS             5359..5697
                     /locus_tag="PWY89_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065748.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5791..6021
                     /locus_tag="PWY89_000691"
     CDS             5791..6021
                     /locus_tag="PWY89_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065749.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6421..6792
                     /locus_tag="PWY89_000692"
     CDS             6421..6792
                     /locus_tag="PWY89_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2065750.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(7066..7758)
                     /locus_tag="PWY89_000693"
     CDS             complement(7066..7758)
                     /locus_tag="PWY89_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2065751.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7761..8546)
                     /locus_tag="PWY89_000694"
     CDS             complement(7761..8546)
                     /locus_tag="PWY89_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2065752.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8783..10183
                     /gene="lacG"
                     /locus_tag="PWY89_000695"
     CDS             8783..10183
                     /gene="lacG"
                     /locus_tag="PWY89_000695"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2065753.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10208..10525
                     /locus_tag="PWY89_000696"
     CDS             10208..10525
                     /locus_tag="PWY89_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2065754.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10538..12211
                     /locus_tag="PWY89_000697"
     CDS             10538..12211
                     /locus_tag="PWY89_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL2065755.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12225..13856
                     /locus_tag="PWY89_000698"
     CDS             12225..13856
                     /locus_tag="PWY89_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL2065756.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14347..15912)
                     /gene="guaA"
                     /locus_tag="PWY89_000699"
     CDS             complement(14347..15912)
                     /gene="guaA"
                     /locus_tag="PWY89_000699"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2065757.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15971..16894)
                     /locus_tag="PWY89_000700"
     CDS             complement(15971..16894)
                     /locus_tag="PWY89_000700"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2065758.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            17090..17695
                     /locus_tag="PWY89_000701"
     CDS             17090..17695
                     /locus_tag="PWY89_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2065759.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            18003..19019
                     /locus_tag="PWY89_000702"
     CDS             18003..19019
                     /locus_tag="PWY89_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2065760.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            19160..20461
                     /locus_tag="PWY89_000703"
     CDS             19160..20461
                     /locus_tag="PWY89_000703"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL2065761.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20507..21169
                     /gene="deoC"
                     /locus_tag="PWY89_000704"
     CDS             20507..21169
                     /gene="deoC"
                     /locus_tag="PWY89_000704"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2065762.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            21190..21588
                     /locus_tag="PWY89_000705"
     CDS             21190..21588
                     /locus_tag="PWY89_000705"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2065763.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21654..22736
                     /locus_tag="PWY89_000706"
     CDS             21654..22736
                     /locus_tag="PWY89_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2065764.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22948..24510
                     /locus_tag="PWY89_000707"
     CDS             22948..24510
                     /locus_tag="PWY89_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065765.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24503..25636
                     /locus_tag="PWY89_000708"
     CDS             24503..25636
                     /locus_tag="PWY89_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065766.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25636..26592
                     /locus_tag="PWY89_000709"
     CDS             25636..26592
                     /locus_tag="PWY89_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065767.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26691..27857
                     /gene="deoB"
                     /locus_tag="PWY89_000710"
     CDS             26691..27857
                     /gene="deoB"
                     /locus_tag="PWY89_000710"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL2065768.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27881..28699
                     /locus_tag="PWY89_000711"
     CDS             27881..28699
                     /locus_tag="PWY89_000711"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2065769.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28716..29423
                     /gene="deoD"
                     /locus_tag="PWY89_000712"
     CDS             28716..29423
                     /gene="deoD"
                     /locus_tag="PWY89_000712"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2065770.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29721..30407)
                     /gene="gpmA"
                     /locus_tag="PWY89_000713"
     CDS             complement(29721..30407)
                     /gene="gpmA"
                     /locus_tag="PWY89_000713"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2065771.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30579..31160)
                     /locus_tag="PWY89_000714"
     CDS             complement(30579..31160)
                     /locus_tag="PWY89_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2065772.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31293..31976)
                     /gene="rpiA"
                     /locus_tag="PWY89_000715"
     CDS             complement(31293..31976)
                     /gene="rpiA"
                     /locus_tag="PWY89_000715"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2065773.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32295..32708
                     /gene="rpsL"
                     /locus_tag="PWY89_000716"
     CDS             32295..32708
                     /gene="rpsL"
                     /locus_tag="PWY89_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2065774.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32792..33262
                     /gene="rpsG"
                     /locus_tag="PWY89_000717"
     CDS             32792..33262
                     /gene="rpsG"
                     /locus_tag="PWY89_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2065775.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33356..35443
                     /gene="fusA"
                     /locus_tag="PWY89_000718"
     CDS             33356..35443
                     /gene="fusA"
                     /locus_tag="PWY89_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2065776.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35615..36802
                     /gene="tuf"
                     /locus_tag="PWY89_000719"
     CDS             35615..36802
                     /gene="tuf"
                     /locus_tag="PWY89_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2065777.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            37209..37517
                     /gene="rpsJ"
                     /locus_tag="PWY89_000720"
     CDS             37209..37517
                     /gene="rpsJ"
                     /locus_tag="PWY89_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2065778.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37548..38177
                     /gene="rplC"
                     /locus_tag="PWY89_000721"
     CDS             37548..38177
                     /gene="rplC"
                     /locus_tag="PWY89_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2065779.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            38205..38828
                     /gene="rplD"
                     /locus_tag="PWY89_000722"
     CDS             38205..38828
                     /gene="rplD"
                     /locus_tag="PWY89_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2065780.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38828..39118
                     /gene="rplW"
                     /locus_tag="PWY89_000723"
     CDS             38828..39118
                     /gene="rplW"
                     /locus_tag="PWY89_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2065781.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            39157..39990
                     /gene="rplB"
                     /locus_tag="PWY89_000724"
     CDS             39157..39990
                     /gene="rplB"
                     /locus_tag="PWY89_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2065782.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            40033..40311
                     /gene="rpsS"
                     /locus_tag="PWY89_000725"
     CDS             40033..40311
                     /gene="rpsS"
                     /locus_tag="PWY89_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2065783.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40334..40681
                     /gene="rplV"
                     /locus_tag="PWY89_000726"
     CDS             40334..40681
                     /gene="rplV"
                     /locus_tag="PWY89_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2065784.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40695..41351
                     /gene="rpsC"
                     /locus_tag="PWY89_000727"
     CDS             40695..41351
                     /gene="rpsC"
                     /locus_tag="PWY89_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2065785.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41354..41788
                     /gene="rplP"
                     /locus_tag="PWY89_000728"
     CDS             41354..41788
                     /gene="rplP"
                     /locus_tag="PWY89_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2065786.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41778..41966
                     /gene="rpmC"
                     /locus_tag="PWY89_000729"
     CDS             41778..41966
                     /gene="rpmC"
                     /locus_tag="PWY89_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2065787.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41991..42257
                     /gene="rpsQ"
                     /locus_tag="PWY89_000730"
     CDS             41991..42257
                     /gene="rpsQ"
                     /locus_tag="PWY89_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2065788.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42315..42683
                     /gene="rplN"
                     /locus_tag="PWY89_000731"
     CDS             42315..42683
                     /gene="rplN"
                     /locus_tag="PWY89_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2065789.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42720..43028
                     /gene="rplX"
                     /locus_tag="PWY89_000732"
     CDS             42720..43028
                     /gene="rplX"
                     /locus_tag="PWY89_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2065790.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            43056..43595
                     /gene="rplE"
                     /locus_tag="PWY89_000733"
     CDS             43056..43595
                     /gene="rplE"
                     /locus_tag="PWY89_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2065791.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43614..43799
                     /locus_tag="PWY89_000734"
     CDS             43614..43799
                     /locus_tag="PWY89_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL2065792.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43835..44233
                     /gene="rpsH"
                     /locus_tag="PWY89_000735"
     CDS             43835..44233
                     /gene="rpsH"
                     /locus_tag="PWY89_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2065793.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            44265..44801
                     /gene="rplF"
                     /locus_tag="PWY89_000736"
     CDS             44265..44801
                     /gene="rplF"
                     /locus_tag="PWY89_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2065794.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44962..45318
                     /gene="rplR"
                     /locus_tag="PWY89_000737"
     CDS             44962..45318
                     /gene="rplR"
                     /locus_tag="PWY89_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2065795.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45339..45839
                     /gene="rpsE"
                     /locus_tag="PWY89_000738"
     CDS             45339..45839
                     /gene="rpsE"
                     /locus_tag="PWY89_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2065796.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45854..46033
                     /gene="rpmD"
                     /locus_tag="PWY89_000739"
     CDS             45854..46033
                     /gene="rpmD"
                     /locus_tag="PWY89_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2065797.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            46077..46517
                     /gene="rplO"
                     /locus_tag="PWY89_000740"
     CDS             46077..46517
                     /gene="rplO"
                     /locus_tag="PWY89_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2065798.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46517..47812
                     /gene="secY"
                     /locus_tag="PWY89_000741"
     CDS             46517..47812
                     /gene="secY"
                     /locus_tag="PWY89_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2065799.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47874..48521
                     /locus_tag="PWY89_000742"
     CDS             47874..48521
                     /locus_tag="PWY89_000742"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2065800.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48712..48930
                     /gene="infA"
                     /locus_tag="PWY89_000743"
     CDS             48712..48930
                     /gene="infA"
                     /locus_tag="PWY89_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2065801.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48963..49079
                     /gene="rpmJ"
                     /locus_tag="PWY89_000744"
     CDS             48963..49079
                     /gene="rpmJ"
                     /locus_tag="PWY89_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2065802.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            49097..49462
                     /gene="rpsM"
                     /locus_tag="PWY89_000745"
     CDS             49097..49462
                     /gene="rpsM"
                     /locus_tag="PWY89_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2065803.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49490..49879
                     /gene="rpsK"
                     /locus_tag="PWY89_000746"
     CDS             49490..49879
                     /gene="rpsK"
                     /locus_tag="PWY89_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2065804.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49959..50897
                     /locus_tag="PWY89_000747"
     CDS             49959..50897
                     /locus_tag="PWY89_000747"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2065805.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50925..51305
                     /gene="rplQ"
                     /locus_tag="PWY89_000748"
     CDS             50925..51305
                     /gene="rplQ"
                     /locus_tag="PWY89_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2065806.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51577..52416
                     /locus_tag="PWY89_000749"
     CDS             51577..52416
                     /locus_tag="PWY89_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2065807.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52392..53261
                     /locus_tag="PWY89_000750"
     CDS             52392..53261
                     /locus_tag="PWY89_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2065808.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            53251..54048
                     /locus_tag="PWY89_000751"
     CDS             53251..54048
                     /locus_tag="PWY89_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2065809.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        17296 a         9686 c        11649 g        15562 t
ORIGIN      
        1 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
       61 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
      121 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
      181 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
      241 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
      301 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
      361 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
      421 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
      481 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
      541 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
      601 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
      661 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
      721 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
      781 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
      841 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
      901 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
      961 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     1021 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     1081 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     1141 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     1201 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     1261 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     1321 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     1381 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     1441 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     1501 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     1561 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     1621 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     1681 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     1741 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     1801 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     1861 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
     1921 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
     1981 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
     2041 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
     2101 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
     2161 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
     2221 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
     2281 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
     2341 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
     2401 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
     2461 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
     2521 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
     2581 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
     2641 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
     2701 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
     2761 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
     2821 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
     2881 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
     2941 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
     3001 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
     3061 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
     3121 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
     3181 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
     3241 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
     3301 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
     3361 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
     3421 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
     3481 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
     3541 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
     3601 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
     3661 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
     3721 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
     3781 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
     3841 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
     3901 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
     3961 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
     4021 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
     4081 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
     4141 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
     4201 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
     4261 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
     4321 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
     4381 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
     4441 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
     4501 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
     4561 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
     4621 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
     4681 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
     4741 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
     4801 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
     4861 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
     4921 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
     4981 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
     5041 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
     5101 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
     5161 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
     5221 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
     5281 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
     5341 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
     5401 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
     5461 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
     5521 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
     5581 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
     5641 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
     5701 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
     5761 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
     5821 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
     5881 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
     5941 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
     6001 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
     6061 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
     6121 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
     6181 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
     6241 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
     6301 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
     6361 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
     6421 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
     6481 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
     6541 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
     6601 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
     6661 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
     6721 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
     6781 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
     6841 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
     6901 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
     6961 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
     7021 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
     7081 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
     7141 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
     7201 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
     7261 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
     7321 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
     7381 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
     7441 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
     7501 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
     7561 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
     7621 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
     7681 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
     7741 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
     7801 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
     7861 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
     7921 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
     7981 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
     8041 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
     8101 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
     8161 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
     8221 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
     8281 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
     8341 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
     8401 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
     8461 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
     8521 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
     8581 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
     8641 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
     8701 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
     8761 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
     8821 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
     8881 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
     8941 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
     9001 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
     9061 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
     9121 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
     9181 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
     9241 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
     9301 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
     9361 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
     9421 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
     9481 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
     9541 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
     9601 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
     9661 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
     9721 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
     9781 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
     9841 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
     9901 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
     9961 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    10021 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    10081 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    10141 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    10201 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    10261 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    10321 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    10381 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    10441 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    10501 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    10561 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    10621 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    10681 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    10741 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    10801 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    10861 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    10921 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    10981 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    11041 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    11101 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    11161 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    11221 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    11281 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    11341 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    11401 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    11461 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    11521 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    11581 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    11641 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    11701 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    11761 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    11821 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    11881 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    11941 tgggatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    12001 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    12061 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    12121 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    12181 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    12241 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    12301 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    12361 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    12421 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    12481 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    12541 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    12601 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    12661 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    12721 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    12781 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    12841 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    12901 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    12961 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    13021 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    13081 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    13141 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    13201 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    13261 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    13321 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    13381 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    13441 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    13501 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    13561 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    13621 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    13681 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    13741 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    13801 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    13861 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    13921 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    13981 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    14041 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    14101 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    14161 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    14221 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    14281 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    14341 ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg
    14401 gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg
    14461 gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt
    14521 gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt
    14581 gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc
    14641 atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc
    14701 aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt
    14761 ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg
    14821 catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt
    14881 accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc
    14941 accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt
    15001 acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt
    15061 gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc
    15121 gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt
    15181 ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt
    15241 tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt
    15301 ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta
    15361 ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg
    15421 gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt
    15481 agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa
    15541 gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg
    15601 ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac
    15661 aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact
    15721 gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt
    15781 aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa
    15841 ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc
    15901 aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta
    15961 ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat
    16021 ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat
    16081 tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa
    16141 aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat
    16201 ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa
    16261 agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga
    16321 tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat
    16381 gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga
    16441 tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt
    16501 ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt
    16561 tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta
    16621 cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac
    16681 ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag
    16741 gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat
    16801 ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc
    16861 ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa
    16921 tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct
    16981 ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata
    17041 cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca
    17101 ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa
    17161 aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta
    17221 tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat
    17281 cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg
    17341 gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa
    17401 gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga
    17461 agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca
    17521 tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat
    17581 ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc
    17641 agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc
    17701 tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag
    17761 ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata
    17821 gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa
    17881 ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc
    17941 tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata
    18001 ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct
    18061 cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt
    18121 cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac
    18181 attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga
    18241 tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc
    18301 gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg
    18361 gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt
    18421 atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc
    18481 ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc
    18541 atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca
    18601 acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac
    18661 atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag
    18721 atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg
    18781 atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc
    18841 tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt gatattggat
    18901 tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca
    18961 aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac
    19021 tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt
    19081 agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga
    19141 agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa
    19201 cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc
    19261 agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga
    19321 aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct
    19381 tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat
    19441 tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct
    19501 aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct
    19561 ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc
    19621 aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt
    19681 tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga
    19741 tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc
    19801 aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc
    19861 ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt
    19921 cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc
    19981 cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac
    20041 gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca
    20101 aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta
    20161 cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt
    20221 aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca
    20281 cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact
    20341 attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa
    20401 tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata
    20461 aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg
    20521 aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat
    20581 cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct
    20641 agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct
    20701 aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc
    20761 agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt
    20821 ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat
    20881 tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc
    20941 tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga
    21001 ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg
    21061 tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac
    21121 aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag
    21181 gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat
    21241 aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc
    21301 aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag
    21361 cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta
    21421 gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa
    21481 ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg
    21541 accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt
    21601 cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa
    21661 aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat
    21721 gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg
    21781 cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg
    21841 aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat
    21901 atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg
    21961 gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa
    22021 caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg
    22081 ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt
    22141 atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc
    22201 gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa
    22261 tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg
    22321 ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta
    22381 acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta
    22441 tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa
    22501 acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa
    22561 aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg
    22621 gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa
    22681 aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc
    22741 atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat
    22801 tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa
    22861 tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga
    22921 tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag
    22981 aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag
    23041 gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac
    23101 gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta
    23161 aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt
    23221 atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc
    23281 gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat
    23341 ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg
    23401 gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca
    23461 gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa
    23521 gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac
    23581 cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc
    23641 tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa
    23701 gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt
    23761 ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc
    23821 gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag
    23881 gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa
    23941 atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta
    24001 gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa
    24061 aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat
    24121 gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa
    24181 aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca
    24241 actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg
    24301 gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg
    24361 gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca
    24421 tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag
    24481 ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt
    24541 gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg
    24601 ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg
    24661 gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt
    24721 agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat
    24781 cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt
    24841 atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg
    24901 agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata
    24961 cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc
    25021 ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg
    25081 cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct
    25141 gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga atccttttgc
    25201 ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg
    25261 cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt
    25321 tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg
    25381 atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact
    25441 tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat
    25501 tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg
    25561 gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga
    25621 agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg
    25681 gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg
    25741 atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc
    25801 aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt
    25861 ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct
    25921 gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg
    25981 atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt
    26041 tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc
    26101 ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg
    26161 aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt
    26221 atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg
    26281 ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga
    26341 caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg
    26401 ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca
    26461 gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt
    26521 gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc
    26581 aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc
    26641 gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac
    26701 gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt
    26761 tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc
    26821 cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga
    26881 aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga
    26941 caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg
    27001 gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta
    27061 acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag
    27121 gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa
    27181 tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg
    27241 atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca
    27301 cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt
    27361 ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg
    27421 gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc
    27481 tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg
    27541 cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact
    27601 tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg
    27661 atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat
    27721 tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata
    27781 tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct
    27841 tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat
    27901 gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt
    27961 gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc
    28021 ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt
    28081 ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga
    28141 aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca
    28201 ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt
    28261 gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga
    28321 agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc
    28381 aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg
    28441 ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc
    28501 cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa
    28561 agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca
    28621 tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga
    28681 aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag
    28741 aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg
    28801 cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta
    28861 ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag
    28921 ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag
    28981 gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg
    29041 cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca
    29101 gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc
    29161 atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc
    29221 aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag
    29281 gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg
    29341 gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat
    29401 tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa
    29461 gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa
    29521 tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca
    29581 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
    29641 gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat cgtgtcacag
    29701 tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa gtcatcattt
    29761 aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc ttcgtcagag
    29821 ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc tactaaaaca
    29881 gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa agcgcgttct
    29941 aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat tgcgtaacga
    30001 cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag agtatcgtaa
    30061 gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc
    30121 aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc
    30181 caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg agttaaaaca
    30241 gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc gccttctttt
    30301 gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa ttggttcaat
    30361 gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt tagctctcct
    30421 tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca attttttgga
    30481 agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga gaatataaaa
    30541 aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa ttgctcgatt
    30601 tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc gcattttgaa
    30661 gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt tttgatcgtt
    30721 tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc atctaatcca
    30781 actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc cattaattgg
    30841 aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat caagtgcagc
    30901 ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt gcctaatcga
    30961 tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa
    31021 tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat ttcttgacaa
    31081 ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt
    31141 gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa ctggatataa
    31201 cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt
    31261 ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc gtcaagaatc
    31321 tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa aagcccatgt
    31381 tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg ttcgatgact
    31441 tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc tccgttttcg
    31501 tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag
    31561 ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac taatttagaa
    31621 gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc gaaaagcaaa
    31681 gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc accgtctatt
    31741 gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa tttttccgct
    31801 tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc ttcacgcata
    31861 cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag acctacgatc
    31921 atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa attcatcttt
    31981 gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc gtgatttacg
    32041 gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa ttgcatctct
    32101 tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta caggtttctg
    32161 tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga cattgcattt
    32221 attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa
    32281 ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt aaatcaaagg
    32341 tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag aaaactcaaa
    32401 ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca atgacaccta
    32461 aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag
    32521 ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt gtattattac
    32581 gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc ggtgctttag
    32641 atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact aaaaaaccta
    32701 aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga agtaattact
    32761 atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg
    32821 tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga tcaaccgtgt
    32881 aatggttgac ggaaaacgcg ggattgcagc taacattatc tataatgcct ttgacttgat
    32941 caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga aaaacgttca
    33001 acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag taccagttga
    33061 agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact acgctcgctt
    33121 gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa
    33181 cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg ccaaccgtgc
    33241 gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata agtcgacctc
    33301 agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa ggagaatgac
    33361 cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca tcgatgctgg
    33421 taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata aaattggtga
    33481 aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac gtggtattac
    33541 gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca tcatcgacac
    33601 accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc
    33661 tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag tttggcgtca
    33721 agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc
    33781 tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg ctcacccaat
    33841 ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg taacaatgaa
    33901 ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa ttcctgaaga
    33961 ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag ctgaaactga
    34021 tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg agttaaaaga
    34081 aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg gttcagcatt
    34141 taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc catcaccgtt
    34201 agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag aacgtcctgc
    34261 agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc cattcgtagg
    34321 tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat acgtacaaaa
    34381 cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg ccaactctcg
    34441 ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt tgaaagacac
    34501 aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat ccatggaatt
    34561 cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc aagataaaat
    34621 gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg aaacaaacgt
    34681 ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata tcatcgttga
    34741 ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag tatcttaccg
    34801 tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa
    34861 aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg ctggtttcga
    34921 atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag ctgttgaaac
    34981 tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag tagatatcaa
    35041 agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg ccttccgtgt
    35101 tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa tccttgaacc
    35161 aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta tgggtcacgt
    35221 aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt
    35281 tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc gttctgctac
    35341 tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac caaaatcagt
    35401 acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttcttg aaaccggata
    35461 gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt gtaaaataat
    35521 tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc agacaaataa
    35581 aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat ttgaccgttc
    35641 taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa ctacattaac
    35701 agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg cttacgatca
    35761 aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag ctcacgttga
    35821 gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg cggattacgt
    35881 taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag tttctgctgc
    35941 tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc
    36001 ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat tactagaatt
    36061 agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg acgatgttcc
    36121 tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg aagaaaaaat
    36181 tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg acaacgacaa
    36241 accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta ctgttgctac
    36301 aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc
    36361 tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat tgttagacta
    36421 cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg aagacatcca
    36481 acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat tctctgcaga
    36541 agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca ctaactaccg
    36601 tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac cagaaggaac
    36661 tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc acccaatcgc
    36721 tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt
    36781 cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca tttattcgga
    36841 cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta tttattgcag
    36901 gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa agagtcagta
    36961 ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt
    37021 gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc acagacgtaa
    37081 tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac gctttgccac
    37141 gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata ggcgaaggag
    37201 ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa caccgtattt
    37261 tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg
    37321 gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg actcataaat
    37381 acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc gacattgtga
    37441 acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct ggtgtaaaca
    37501 tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg accaaaggaa
    37561 tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa ttaattccag
    37621 taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg gaaacggatg
    37681 gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca aacaaacctg
    37741 cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa
    37801 atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt ttccaagcag
    37861 gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg gttatcaaac
    37921 gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt cgtcctgggt
    37981 caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg
    38041 gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg
    38101 ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact atcaaatcag
    38161 ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg aatgtagcat
    38221 tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa atcttcggaa
    38281 ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg tcattaagac
    38341 aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc
    38401 gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa tggcgtggag
    38461 gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct aaaaaagttc
    38521 gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa
    38581 tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt cttgctaact
    38641 tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt gctgctttat
    38701 cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt gtgctagacg
    38761 ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt gaggaggtgc
    38821 ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa aatcaatgct
    38881 tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca aaactttagt
    38941 aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt
    39001 acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc
    39061 gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg ctgaataagc
    39121 actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct
    39181 acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac aacatcaaca
    39241 cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa caacggccgc
    39301 atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat cgacttcaaa
    39361 cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc aaaccgttca
    39421 gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt agcacctaaa
    39481 ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa agtcggaaat
    39541 gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga aatgaaacct
    39601 ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa
    39661 ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt agctgcatgc
    39721 cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat cggtaaagct
    39781 ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat gaaccctaat
    39841 gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc accagtttct
    39901 ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa atcagacaaa
    39961 cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc acattttgag
    40021 aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga gcatttaatg
    40081 aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc
    40141 cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga tggacgtaaa
    40201 catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga atttgcacct
    40261 acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta atttgagggg
    40321 aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa aaacagttcg
    40381 cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga
    40441 tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg aaaaagtatt
    40501 gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa acttggtagt
    40561 aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg
    40621 atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat cagaaaaata
    40681 aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta ggcatcatcc
    40741 gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta cacgaagatt
    40801 taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca acagttgaaa
    40861 tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca ggtatggtta
    40921 ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa ttaactggta
    40981 aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca aaattagtag
    41041 gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag
    41101 ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta tctggtcgtc
    41161 taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt cctcttcata
    41221 ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac ggaaaactag
    41281 gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac actgagaaag
    41341 gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa
    41401 aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc
    41461 tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg caatgactcg
    41521 ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat cttatactgc
    41581 caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat gggttgcacc
    41641 agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga
    41701 agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa aacgtgagga
    41761 aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact gccgaaatgc
    41821 ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa ttagcaacag
    41881 gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa
    41941 ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt atgactgaag
    42001 gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa
    42061 ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc atgaactatt
    42121 ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac atcgtgagaa
    42181 ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa gtagtcgaag
    42241 aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca aaatctgaaa
    42301 ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg
    42361 cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact gctaacatcg
    42421 gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc aaaaaaggtg
    42481 acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat
    42541 atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc cctcgtggaa
    42601 cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt
    42661 cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc gaaacagtta
    42721 tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa aacaaagaag
    42781 gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg
    42841 ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt gagatggaag
    42901 cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct ggccgagttg
    42961 gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag
    43021 ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc
    43081 ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt atgcaaacac
    43141 ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca aacgctaaaa
    43201 acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca atgattacaa
    43261 aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag
    43321 ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct gtttcactac
    43381 cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata
    43441 ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta gtagataaag
    43501 tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt
    43561 tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac tacgtggcta
    43621 aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc
    43681 gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt tgccgtattt
    43741 gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa
    43801 aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc caattgcaga
    43861 ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag aagtacctgc
    43921 atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt
    43981 tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa
    44041 cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt
    44101 caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca tttcgacttc
    44161 tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc
    44221 ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa
    44281 ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac aacgttacag
    44341 ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa atgaatatcg
    44401 aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa acaattcatg
    44461 gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag
    44521 cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa cttgtattga
    44581 acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact gttgaagtac
    44641 cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc gaattagctg
    44701 caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt cgttatgttg
    44761 gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat
    44821 agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa aatacatttt
    44881 tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata gtagtacaat
    44941 aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac acgtcaaaag
    45001 agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg cttgaacgtt
    45061 tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg tgtaacgcta
    45121 gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga agcagcacaa
    45181 gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt
    45241 gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa
    45301 aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc gatccaaaac
    45361 atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg
    45421 gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga cacgtaggat
    45481 ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt gaagatgcga
    45541 agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa gtaatcggtg
    45601 cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg gttgctgctg
    45661 gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact tctaaatctt
    45721 taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg aaacaattga
    45781 aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta atcggataag
    45841 gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta tcggacgtcc
    45901 tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca gttcagtggt
    45961 taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc tagtggacgt
    46021 tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc aaacctatga
    46081 aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg
    46141 gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt
    46201 caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc cgtcgtttac
    46261 caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat cttgatgtat
    46321 tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg
    46381 tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac aagaaattaa
    46441 ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg gctggtggat
    46501 caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa aagacattag
    46561 atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg cacatattac
    46621 tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat ttttcaacat
    46681 gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa tgggggtttc
    46741 accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg tgcctcgatt
    46801 tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata
    46861 tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat tcaatcaata
    46921 cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga tcggtttgat
    46981 tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag aaaaaggtat
    47041 cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc ctgaaagtat
    47101 cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat ggaaatctgc
    47161 tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg taacattttt
    47221 ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac
    47281 aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta tctttgctag
    47341 ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat tcggttctga
    47401 aaactggtac aaagtgatga tggagatctt taactataat acagcacctg gtgctacgat
    47461 ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag taaaccctga
    47521 gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac gccctggtaa
    47581 ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg gtgcagtctt
    47641 ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc ttccacaatc
    47701 aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag aaactgcgaa
    47761 acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt
    47821 gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa acaatgaacc
    47881 tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa atcattgatg
    47941 cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg caaaacgaaa
    48001 cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt cctgatgaag
    48061 taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa ggcttcttat
    48121 tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg ctaaaagatt
    48181 tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac
    48241 gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa attttcaacc
    48301 ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat caaagagaag
    48361 atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa
    48421 tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct cgtgaaatcg
    48481 atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc ccgtagccga
    48541 cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata tgtcagtccg
    48601 acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga actgcagttt
    48661 ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa
    48721 gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa
    48781 gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat tcgtatgcat
    48841 tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata cgatcttaac
    48901 cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca ggaggtatta
    48961 tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta
    49021 aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt caaggataat
    49081 tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg ataaacgtgt
    49141 agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa agatcttagc
    49201 tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc
    49261 tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt
    49321 gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc gccgtggatt
    49381 gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc
    49441 tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca tggcagcaaa
    49501 aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg cacatatcca
    49561 ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg cattagcgtg
    49621 gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca
    49681 aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga ctgttgaagt
    49741 aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac aagctacagg
    49801 tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg cccgccctcc
    49861 aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta acgtcattga
    49921 acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc gaaaaaccaa
    49981 gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa
    50041 gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct tctttgccag
    50101 gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct accatcaaag
    50161 gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg ctgaaaatgt
    50221 atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca gtaactgctg
    50281 gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac atctgtagtg
    50341 tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt ggttatgttc
    50401 aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc gattcaatct
    50461 acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga caccgtgaag
    50521 attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg ttggaagcaa
    50581 tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac ttaactgatg
    50641 aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg
    50701 aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg aaacgtgcag
    50761 gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc aaagtccgta
    50821 acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta ggtttaggtt
    50881 tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt tatcgtaaac
    50941 taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact gatttattaa
    51001 tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca actgcagaaa
    51061 aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg
    51121 tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt
    51181 tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc ggatacacac
    51241 gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc ttagaacttg
    51301 tctaaaatca cactaataac atcactttat agatgattct tttagagtgt tatgatgttg
    51361 gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg cttggggagt
    51421 aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac agatatgttt
    51481 gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt tttttgatag
    51541 tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat tgaattagaa
    51601 aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga tgtctccttt
    51661 acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg aaaatcgaca
    51721 cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa agtgggaaat
    51781 caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat ggtttttcaa
    51841 aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt tggtcttgaa
    51901 aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt
    51961 agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca aaaacaacgt
    52021 gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga tgaagcaacc
    52081 agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa
    52141 gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc taatgccaat
    52201 cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa agaaattttt
    52261 tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga aaaactgaag
    52321 agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga agaaaggatg
    52381 gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag ccgaatacac
    52441 catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac agttatactg
    52501 cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg aatgcgcttg
    52561 ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg gatacggata
    52621 ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac
    52681 agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca
    52741 gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg ctggatgaaa
    52801 gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga gtagcaatcg
    52861 ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg gcaggacttg
    52921 atccccaggg cagaaaagaa atgatggaga tgttttggcg tcttcataag gaacagcaga
    52981 tcactattgt attagttacg catttaatgg atgatgtagc caattttgca gattatgtct
    53041 atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc tttcaaaaca
    53101 ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt gcagaagaat
    53161 taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa ttagcagacg
    53221 ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt tggtcgttat
    53281 atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt agccagtttt
    53341 tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt gttggcattt
    53401 ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat ccgaggcatt
    53461 cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt tacaagtggc
    53521 ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt gatgaacggc
    53581 ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact cacgcttact
    53641 acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta
    53701 cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca
    53761 ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt
    53821 gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc tttatttgtc
    53881 agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg ctacaaaggt
    53941 ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac gatggttatc
    54001 attgcttttg tattattaac tgtgctactg atctttttaa gaggataaac gttcagtggg
    54061 ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc agtgtatgac
    54121 aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt atctgaaaag
    54181 aaagaacgaa aaa
//
LOCUS       DANKBF010000012        53719 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000012 DANKBF010000000
VERSION     DANKBF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            160..840
                     /locus_tag="PWY89_000752"
     CDS             160..840
                     /locus_tag="PWY89_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2065810.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="PWY89_000753"
     CDS             930..1964
                     /locus_tag="PWY89_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2065811.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="PWY89_000754"
     CDS             1978..2313
                     /locus_tag="PWY89_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065812.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="PWY89_000755"
     CDS             2297..4777
                     /locus_tag="PWY89_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065813.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="PWY89_000756"
     CDS             4833..6392
                     /locus_tag="PWY89_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065814.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="PWY89_000757"
     CDS             6389..7753
                     /locus_tag="PWY89_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2065815.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="PWY89_000758"
     CDS             7924..8994
                     /locus_tag="PWY89_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065816.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="PWY89_000759"
     CDS             9009..9677
                     /locus_tag="PWY89_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065817.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="PWY89_000760"
     CDS             9697..10275
                     /locus_tag="PWY89_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2065818.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="PWY89_000761"
     CDS             10676..11587
                     /locus_tag="PWY89_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2065819.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="PWY89_000762"
     CDS             complement(11965..12882)
                     /locus_tag="PWY89_000762"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL2065820.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="PWY89_000763"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="PWY89_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2065821.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="PWY89_000764"
     CDS             13579..14127
                     /locus_tag="PWY89_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL2065822.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="PWY89_000765"
     CDS             14242..16602
                     /locus_tag="PWY89_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2065823.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="PWY89_000766"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="PWY89_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2065824.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="PWY89_000767"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="PWY89_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2065825.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="PWY89_000768"
     CDS             19108..20007
                     /locus_tag="PWY89_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065826.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="PWY89_000769"
     CDS             20000..21262
                     /locus_tag="PWY89_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065827.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="PWY89_000770"
     CDS             21423..23321
                     /locus_tag="PWY89_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL2065828.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="PWY89_000771"
     CDS             complement(23651..24370)
                     /locus_tag="PWY89_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL2065829.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="PWY89_000772"
     CDS             24707..25039
                     /locus_tag="PWY89_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065830.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="PWY89_000773"
     CDS             25026..27029
                     /locus_tag="PWY89_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL2065831.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="PWY89_000774"
     CDS             27048..27518
                     /locus_tag="PWY89_000774"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL2065832.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="PWY89_000775"
     CDS             27539..28123
                     /locus_tag="PWY89_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL2065833.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="PWY89_000776"
     CDS             28138..29124
                     /locus_tag="PWY89_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL2065834.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="PWY89_000777"
     CDS             29114..29425
                     /locus_tag="PWY89_000777"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL2065835.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="PWY89_000778"
     CDS             29447..31228
                     /locus_tag="PWY89_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL2065836.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="PWY89_000779"
     CDS             31221..32597
                     /locus_tag="PWY89_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL2065837.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="PWY89_000780"
     CDS             32604..33239
                     /locus_tag="PWY89_000780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL2065838.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="PWY89_000781"
     CDS             33269..34624
                     /locus_tag="PWY89_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL2065839.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="PWY89_000782"
     CDS             complement(34816..36210)
                     /locus_tag="PWY89_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2065840.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="PWY89_000783"
     CDS             36302..37273
                     /locus_tag="PWY89_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2065841.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="PWY89_000784"
     CDS             37270..38052
                     /locus_tag="PWY89_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL2065842.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="PWY89_000785"
     CDS             complement(38221..38592)
                     /locus_tag="PWY89_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2065843.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="PWY89_000786"
     CDS             38726..39169
                     /locus_tag="PWY89_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2065844.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="PWY89_000787"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="PWY89_000787"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL2065845.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="PWY89_000788"
     CDS             complement(40040..41407)
                     /locus_tag="PWY89_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2065846.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="PWY89_000789"
     CDS             complement(41391..42221)
                     /locus_tag="PWY89_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL2065847.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="PWY89_000790"
     CDS             42384..42821
                     /locus_tag="PWY89_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL2065848.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /locus_tag="PWY89_000791"
     CDS             42897..43517
                     /locus_tag="PWY89_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL2065849.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="PWY89_000792"
     CDS             43706..44212
                     /locus_tag="PWY89_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL2065850.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="PWY89_000793"
     CDS             44229..45128
                     /locus_tag="PWY89_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2065851.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="PWY89_000794"
     CDS             45215..46099
                     /locus_tag="PWY89_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2065852.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46291..46362)
                     /locus_tag="PWY89_000795"
     tRNA            complement(46291..46362)
                     /locus_tag="PWY89_000795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46327..46329),aa:Arg,seq:ccg)
     gene            46422..47756
                     /gene="rpoN"
                     /locus_tag="PWY89_000796"
     CDS             46422..47756
                     /gene="rpoN"
                     /locus_tag="PWY89_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL2065853.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47905..48996
                     /locus_tag="PWY89_000797"
     CDS             47905..48996
                     /locus_tag="PWY89_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL2065854.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49037..50038
                     /gene="gap"
                     /locus_tag="PWY89_000798"
     CDS             49037..50038
                     /gene="gap"
                     /locus_tag="PWY89_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2065855.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50148..51338
                     /locus_tag="PWY89_000799"
     CDS             50148..51338
                     /locus_tag="PWY89_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2065856.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51442..52197
                     /locus_tag="PWY89_000800"
     CDS             51442..52197
                     /locus_tag="PWY89_000800"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2065857.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52313..53611
                     /gene="eno"
                     /locus_tag="PWY89_000801"
     CDS             52313..53611
                     /gene="eno"
                     /locus_tag="PWY89_000801"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2065858.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17827 a         9355 c        11351 g        15186 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttagttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgacttgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta
    46261 ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc
    46321 aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt
    46381 aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt
    46441 tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca
    46501 tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg
    46561 aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt
    46621 catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt
    46681 ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga
    46741 aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag
    46801 aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga
    46861 ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc
    46921 aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg
    46981 aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg
    47041 ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg
    47101 accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa
    47161 atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact
    47221 tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac
    47281 aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg
    47341 ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc
    47401 ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg
    47461 cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca
    47521 ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga
    47581 agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga
    47641 aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat
    47701 atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag
    47761 tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca
    47821 aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat
    47881 agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt
    47941 ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat
    48001 attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg
    48061 attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa
    48121 acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca
    48181 actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg
    48241 catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca
    48301 ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc
    48361 ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg
    48421 gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc
    48481 cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga
    48541 atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg
    48601 gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt
    48661 gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag
    48721 atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc
    48781 tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg
    48841 caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa
    48901 gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct
    48961 gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat
    49021 aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg
    49081 tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt
    49141 gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa
    49201 cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt
    49261 agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga
    49321 atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa
    49381 acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa
    49441 ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg
    49501 tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac
    49561 aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga
    49621 cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa
    49681 agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt
    49741 tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt
    49801 tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga
    49861 cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca
    49921 aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa
    49981 cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga
    50041 tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc
    50101 gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa
    50161 cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc
    50221 ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca
    50281 actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa
    50341 cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat
    50401 taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc
    50461 gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg
    50521 gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg
    50581 tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg
    50641 cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg
    50701 gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt
    50761 ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg
    50821 gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag
    50881 aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct
    50941 tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg
    51001 aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat
    51061 ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg
    51121 aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag
    51181 gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg
    51241 ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta
    51301 aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct
    51361 gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata
    51421 aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac
    51481 aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat
    51541 gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca
    51601 gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg
    51661 ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc
    51721 ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca
    51781 aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc
    51841 tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat
    51901 ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt
    51961 actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca
    52021 gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct
    52081 gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc
    52141 ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg
    52201 ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc
    52261 gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat
    52321 tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt
    52381 agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac
    52441 tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg
    52501 tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga
    52561 tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa
    52621 aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc
    52681 tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc
    52741 aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca
    52801 agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc
    52861 tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg
    52921 tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat
    52981 cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc
    53041 tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga
    53101 aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc
    53161 aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac
    53221 tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca
    53281 aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca
    53341 aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac
    53401 agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt
    53461 tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa
    53521 atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt
    53581 gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac
    53641 tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa
    53701 atatcagcag gtagagagt
//
LOCUS       DANKBF010000013        52141 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000013 DANKBF010000000
VERSION     DANKBF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            330..1175
                     /locus_tag="PWY89_000802"
     CDS             330..1175
                     /locus_tag="PWY89_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2065859.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="PWY89_000803"
     CDS             1456..2676
                     /locus_tag="PWY89_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL2065860.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="PWY89_000804"
     CDS             2673..5393
                     /locus_tag="PWY89_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2065861.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="PWY89_000805"
     CDS             5377..6321
                     /locus_tag="PWY89_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2065862.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="PWY89_000806"
     CDS             complement(6390..7400)
                     /locus_tag="PWY89_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2065863.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="PWY89_000807"
     CDS             complement(7580..7924)
                     /locus_tag="PWY89_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2065864.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="PWY89_000808"
     CDS             complement(7917..8351)
                     /locus_tag="PWY89_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL2065865.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="PWY89_000809"
     CDS             8489..9226
                     /locus_tag="PWY89_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065866.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="PWY89_000810"
     CDS             9219..10430
                     /locus_tag="PWY89_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065867.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="PWY89_000811"
     CDS             10634..11416
                     /locus_tag="PWY89_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL2065868.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="PWY89_000812"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="PWY89_000812"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2065869.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="PWY89_000813"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="PWY89_000813"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL2065870.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="PWY89_000814"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="PWY89_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2065871.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="PWY89_000815"
     CDS             15018..16067
                     /locus_tag="PWY89_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL2065872.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="PWY89_000816"
     CDS             16064..16342
                     /locus_tag="PWY89_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL2065873.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="PWY89_000817"
     CDS             16317..16787
                     /locus_tag="PWY89_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL2065874.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="PWY89_000818"
     CDS             16804..17106
                     /locus_tag="PWY89_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065875.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="PWY89_000819"
     CDS             17123..17494
                     /locus_tag="PWY89_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL2065876.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="PWY89_000820"
     CDS             17466..17825
                     /locus_tag="PWY89_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065877.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="PWY89_000821"
     CDS             17997..19004
                     /locus_tag="PWY89_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2065878.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="PWY89_000822"
     CDS             19029..20213
                     /locus_tag="PWY89_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2065879.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="PWY89_000823"
     CDS             complement(20650..22233)
                     /locus_tag="PWY89_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2065880.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="PWY89_000824"
     CDS             complement(22811..22954)
                     /locus_tag="PWY89_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL2065881.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="PWY89_000825"
     CDS             complement(23177..23656)
                     /locus_tag="PWY89_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2065882.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="PWY89_000826"
     CDS             complement(23729..24013)
                     /locus_tag="PWY89_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065883.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="PWY89_000827"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWY89_000827"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="PWY89_000828"
     CDS             complement(24357..25643)
                     /locus_tag="PWY89_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2065884.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="PWY89_000829"
     CDS             25781..26452
                     /locus_tag="PWY89_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL2065885.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="PWY89_000830"
     CDS             26418..26906
                     /locus_tag="PWY89_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL2065886.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="PWY89_000831"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="PWY89_000831"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2065887.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="PWY89_000832"
     CDS             27867..28631
                     /locus_tag="PWY89_000832"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2065888.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="PWY89_000833"
     CDS             28864..31077
                     /locus_tag="PWY89_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL2065889.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="PWY89_000834"
     CDS             31088..31534
                     /locus_tag="PWY89_000834"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2065890.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="PWY89_000835"
     CDS             31705..32964
                     /locus_tag="PWY89_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL2065891.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="PWY89_000836"
     CDS             complement(33053..34000)
                     /locus_tag="PWY89_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2065892.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="PWY89_000837"
     CDS             34394..35686
                     /locus_tag="PWY89_000837"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2065893.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="PWY89_000838"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="PWY89_000838"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2065894.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="PWY89_000839"
     CDS             37579..38454
                     /locus_tag="PWY89_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2065895.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="PWY89_000840"
     CDS             38720..40603
                     /locus_tag="PWY89_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2065896.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="PWY89_000841"
     CDS             complement(40678..40962)
                     /locus_tag="PWY89_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2065897.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="PWY89_000842"
     CDS             complement(40952..41686)
                     /locus_tag="PWY89_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL2065898.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="PWY89_000843"
     CDS             41815..42438
                     /locus_tag="PWY89_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL2065899.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="PWY89_000844"
     CDS             42630..43751
                     /locus_tag="PWY89_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL2065900.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="PWY89_000845"
     CDS             43758..46886
                     /locus_tag="PWY89_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL2065901.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="PWY89_000846"
     CDS             46905..50099
                     /locus_tag="PWY89_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL2065902.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="PWY89_000847"
     CDS             complement(50245..50952)
                     /locus_tag="PWY89_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2065903.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51717..52115
                     /gene="spxA"
                     /locus_tag="PWY89_000848"
     CDS             51717..52115
                     /gene="spxA"
                     /locus_tag="PWY89_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2065904.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17111 a         9068 c        10789 g        15173 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaat taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatt ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gtgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ccagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac
    51361 agtattattt aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct
    51421 taagaaatcg gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt
    51481 tcatatgttt agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa
    51541 gcgagaatgc tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat
    51601 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
    51661 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
    51721 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
    51781 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
    51841 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
    51901 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
    51961 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
    52021 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
    52081 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
    52141 a
//
LOCUS       DANKBF010000014        51350 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000014 DANKBF010000000
VERSION     DANKBF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            134..682
                     /gene="aac(6')"
                     /locus_tag="PWY89_000849"
     CDS             134..682
                     /gene="aac(6')"
                     /locus_tag="PWY89_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2065905.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2889
                     /locus_tag="PWY89_000850"
     CDS             1228..2889
                     /locus_tag="PWY89_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2065906.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2959..3306)
                     /locus_tag="PWY89_000851"
     CDS             complement(2959..3306)
                     /locus_tag="PWY89_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL2065907.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            3390..4331
                     /locus_tag="PWY89_000852"
     CDS             3390..4331
                     /locus_tag="PWY89_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065908.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4332..5375
                     /locus_tag="PWY89_000853"
     CDS             4332..5375
                     /locus_tag="PWY89_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065909.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            5381..6451
                     /locus_tag="PWY89_000854"
     CDS             5381..6451
                     /locus_tag="PWY89_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065910.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            6441..7394
                     /locus_tag="PWY89_000855"
     CDS             6441..7394
                     /locus_tag="PWY89_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2065911.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            7406..8416
                     /locus_tag="PWY89_000856"
     CDS             7406..8416
                     /locus_tag="PWY89_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2065912.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(8701..9522)
                     /locus_tag="PWY89_000857"
     CDS             complement(8701..9522)
                     /locus_tag="PWY89_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2065913.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(9619..9897)
                     /locus_tag="PWY89_000858"
     CDS             complement(9619..9897)
                     /locus_tag="PWY89_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065914.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            10165..12174
                     /gene="metG"
                     /locus_tag="PWY89_000859"
     CDS             10165..12174
                     /gene="metG"
                     /locus_tag="PWY89_000859"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2065915.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(12653..13240)
                     /locus_tag="PWY89_000860"
     CDS             complement(12653..13240)
                     /locus_tag="PWY89_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065916.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            13415..14737
                     /locus_tag="PWY89_000861"
     CDS             13415..14737
                     /locus_tag="PWY89_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2065917.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14892..15668
                     /locus_tag="PWY89_000862"
     CDS             14892..15668
                     /locus_tag="PWY89_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2065918.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            15661..16230
                     /gene="rnmV"
                     /locus_tag="PWY89_000863"
     CDS             15661..16230
                     /gene="rnmV"
                     /locus_tag="PWY89_000863"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL2065919.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            16223..17107
                     /gene="rsmA"
                     /locus_tag="PWY89_000864"
     CDS             16223..17107
                     /gene="rsmA"
                     /locus_tag="PWY89_000864"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2065920.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            17558..18187
                     /locus_tag="PWY89_000865"
     CDS             17558..18187
                     /locus_tag="PWY89_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065921.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(18311..19420)
                     /gene="ugpC"
                     /locus_tag="PWY89_000866"
     CDS             complement(18311..19420)
                     /gene="ugpC"
                     /locus_tag="PWY89_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL2065922.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            19629..20051
                     /gene="mgsA"
                     /locus_tag="PWY89_000867"
     CDS             19629..20051
                     /gene="mgsA"
                     /locus_tag="PWY89_000867"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2065923.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            20374..20910
                     /locus_tag="PWY89_000868"
     CDS             20374..20910
                     /locus_tag="PWY89_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2065924.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20950..22713)
                     /locus_tag="PWY89_000869"
     CDS             complement(20950..22713)
                     /locus_tag="PWY89_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065925.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(22716..24431)
                     /locus_tag="PWY89_000870"
     CDS             complement(22716..24431)
                     /locus_tag="PWY89_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065926.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(25210..25434)
                     /locus_tag="PWY89_000871"
     CDS             complement(25210..25434)
                     /locus_tag="PWY89_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065927.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            25625..28402
                     /locus_tag="PWY89_000872"
     CDS             25625..28402
                     /locus_tag="PWY89_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2065928.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            28483..28902
                     /locus_tag="PWY89_000873"
     CDS             28483..28902
                     /locus_tag="PWY89_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL2065929.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            28914..29384
                     /locus_tag="PWY89_000874"
     CDS             28914..29384
                     /locus_tag="PWY89_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2065930.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            29401..30180
                     /locus_tag="PWY89_000875"
     CDS             29401..30180
                     /locus_tag="PWY89_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2065931.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            30167..30988
                     /locus_tag="PWY89_000876"
     CDS             30167..30988
                     /locus_tag="PWY89_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2065932.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            31005..32054
                     /locus_tag="PWY89_000877"
     CDS             31005..32054
                     /locus_tag="PWY89_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2065933.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            32067..33071
                     /locus_tag="PWY89_000878"
     CDS             32067..33071
                     /locus_tag="PWY89_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2065934.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            33704..34723
                     /locus_tag="PWY89_000879"
     CDS             33704..34723
                     /locus_tag="PWY89_000879"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2065935.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            34766..35647
                     /gene="galU"
                     /locus_tag="PWY89_000880"
     CDS             34766..35647
                     /gene="galU"
                     /locus_tag="PWY89_000880"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2065936.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35947..36324
                     /locus_tag="PWY89_000881"
     CDS             35947..36324
                     /locus_tag="PWY89_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065937.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            36930..37544
                     /locus_tag="PWY89_000882"
     CDS             36930..37544
                     /locus_tag="PWY89_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2065938.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            38317..40056
                     /gene="yfcC"
                     /locus_tag="PWY89_000883"
     CDS             38317..40056
                     /gene="yfcC"
                     /locus_tag="PWY89_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2065939.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            40213..41397
                     /gene="iadA"
                     /locus_tag="PWY89_000884"
     CDS             40213..41397
                     /gene="iadA"
                     /locus_tag="PWY89_000884"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL2065940.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            41508..41711
                     /locus_tag="PWY89_000885"
     CDS             41508..41711
                     /locus_tag="PWY89_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2065941.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(41812..42474)
                     /locus_tag="PWY89_000886"
     CDS             complement(41812..42474)
                     /locus_tag="PWY89_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065942.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(42487..43989)
                     /locus_tag="PWY89_000887"
     CDS             complement(42487..43989)
                     /locus_tag="PWY89_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065943.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            44537..46216
                     /locus_tag="PWY89_000888"
     CDS             44537..46216
                     /locus_tag="PWY89_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2065944.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            46343..46822
                     /locus_tag="PWY89_000889"
     CDS             46343..46822
                     /locus_tag="PWY89_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL2065945.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            47041..47721
                     /locus_tag="PWY89_000890"
     CDS             47041..47721
                     /locus_tag="PWY89_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2065946.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            47729..49060
                     /locus_tag="PWY89_000891"
     CDS             47729..49060
                     /locus_tag="PWY89_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2065947.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            49075..50037
                     /locus_tag="PWY89_000892"
     CDS             49075..50037
                     /locus_tag="PWY89_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL2065948.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            50148..50486
                     /locus_tag="PWY89_000893"
     CDS             50148..50486
                     /locus_tag="PWY89_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL2065949.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            50565..51272
                     /locus_tag="PWY89_000894"
     CDS             50565..51272
                     /locus_tag="PWY89_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL2065950.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT        16751 a         8963 c        10687 g        14949 t
ORIGIN      
        1 atttttttat tttataagta agataaataa tgaaagaata gagatactga ttggtagcta
       61 aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca
      121 agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca
      181 gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga
      241 agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt
      301 agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt
      361 agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca attacttaga
      421 aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca
      481 tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat
      541 ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg
      601 tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat
      661 tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa
      721 ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa cttatttccg
      781 aagaagctac ttttggaaca ccgtgtacca gaggttgtga aaaagtcgct tagtttcgag
      841 tagtaagaaa aattttgtga aaatggcttt ctacattttt gcataaattt ggcttaatac
      901 cgagaaactg gcttttgaac accgtttatt cagataaggg actgtgacaa gacttttgtc
      961 acagtttctt tttgtttatt cttttttatc tttgtatggc aataatagac aatataacga
     1021 aaattattta atggcttttg gaagaccatg gaaaaccttg tacaaagcta ttttttctga
     1081 aaaattcctt gaaacctcac gaaaaaatca ttattttatg aaaaagacaa aaaaaagtgt
     1141 tgatttttta ataattattc attaaactct taaacaactt cagaaaatat taattattct
     1201 gaaaattcca ataaaggggt acgtaaaatg aaaaagaaat ttacatttgg tttggttgcg
     1261 gtatgcggcc ttgtacttgc tggatgttat ggcggcagtt cagccacaag cagtagttca
     1321 gcaaaaagcg gttcagcaga tggaggaggc gtatttaact tagtcgtgcc tcaagaaatg
     1381 cctacagctg acttatcagt tgcaacagat acaatcagtt ttacagcatt gaataacgtt
     1441 tatgaaggaa tttaccgtct tgatgaagac agcaaaccac agccggcagg agctagcgag
     1501 ttggcagaag tcagtgaaga cggattaact tacaaaatca aattacgaga agacgcaaaa
     1561 tggtcaaatg gtgatcctgt tacagcagcg gattacgttt atggctggca acgaacagta
     1621 gacccagcta cagcttctga atatgcttac ctgtatgcgc cagttgaaaa tgcagaagac
     1681 attacagctg gaaagaaaga taaatcagaa ctagggatca aagcagttgg tgactatgaa
     1741 ttagaaatca aattgacgaa acaaacacca tatttccaat atctgcttgc tttcccatca
     1801 tttttcccac aaaaacaatc cgtagtggaa gaaaatgggg atgcctatgc atctgcaagt
     1861 gataaagctg tttataatgg accattcaca ttagaagatt ttgatggacc aggaacagat
     1921 acagaatgga cttataagaa aaatgatcat tactgggaca aagatacagt aaaactttct
     1981 gaaatcaaag tcagtgtagt aaaagaatct tctactgcat tgaacttatt caaagatggc
     2041 caagcagatg acgtgatctt gtctggtgag ttagcacagc aaaacgcaaa cgatccagct
     2101 tatacttctg ttaaagaagc gcggacaagc tatatcgaat tcaatcaacg tgaaaagaat
     2161 tcaccattca acaatgtgaa cctaagaaaa gctatttctt attctatcga tcgtgaagct
     2221 ttagtaaaac aagtactagg tgacggatca gttgtctcta caggattgat cccatcagat
     2281 atggtaaaaa gtccaacaac gaacgaagac tttgcaaaag aagctggaaa attagtttct
     2341 tatgacaaag ataaagcaaa agaatattgg gaaaaagcga aaaaagaatt gggcgttgat
     2401 tctttagaat ttgatttgat ggcttctgat gatgattcat cgaaaaaagt aatcgaatat
     2461 gttcaaaact caatccaaga aaacttggat ggcgtaacag tcaaaccaac acctgttcca
     2521 ttctctgtac gtcttgaccg ctcaacttcg ggtgattttg acgcagtact aggcggatgg
     2581 gctgctgact atgcagaccc aagcagcttt actgatttgt ttgtcacagg taattcatac
     2641 aaccgtggtc gctggagcaa tgcagaatat gacgaagcag ttaaagcagc ttcatcaaca
     2701 gacgctggcg atgaacaagc ccgttgggat gatttccaaa aagcaatcaa gatcatctct
     2761 gatgatatgg gtgtcgttcc ggtgtaccaa aaagcagaag gtcacctagt gaatgaaaaa
     2821 atcaaaggta tcgttcacca tgcggcaggc gcttcatggg attacaaatg gacatacgtt
     2881 gaagaataat cgttaatacc gtgagcatga gaaaaaacga ggctgcggca aatgtcgcaa
     2941 cctcgttctc atattcactt atcctaacaa agaaaaaagc atgaaaatag cagaaagagc
     3001 aattagtatt ccggcaatga tcagccaaaa gctgtacata ccgtagctgg cagacgagag
     3061 cgaggaaaat tctggttttt tctttcggtt ccttgttcgt gcaagatgga ccgatcgctg
     3121 aaaatgatcg aagaaacccg aagaaaagac ccataaaaaa ccgccgataa tcagaaacgg
     3181 caatgaccat aagaatagat cattagataa ttgaatgagt gtaaaggaat ggttgatgag
     3241 attttttatt aaaataacca gaatcagtag aaaagctact gcgtagggga tagatttttt
     3301 tttcatttga ttgctccttt atttttggtt cattatctaa taatgtagcg aaacatctgt
     3361 cagaagtcaa aagctagggg gaaacattag tgaatagtta tatcaaatat gtattaaaac
     3421 gtgtcttttt catgatcatc acgctttggt taattgcaac aatcaccttt ttcttgatgc
     3481 aattgctgcc gggcacgcca tatacgaatc aggaacgttt gagtcctgaa acaatcgaga
     3541 tgctaaataa acaagtcggt ttggataaac cagttatcgt acaatatggg atttatcttt
     3601 ctaatttgct tcaaggagat tttggaattt ccttccaatt taaaaaccag ccagtcgcac
     3661 atttgttagc tggaagaatc ggaccatcct tgcagttagg gttacaggca attatctttg
     3721 gaacagtcct agggacgatt ttaggaacga tctctgccat gaagcaaaat acatgggcag
     3781 atacatcttc tacactcgta gcgatattag gtcgttccat tccaaacttt gtatttgctg
     3841 ttttattaca atatatcttt gcaatcaaat tacaagtttt gccaatcgca aaatgggatg
     3901 gctttgtgta cacaatctta ccaacaattg cgttggccat gtcgccgctt gctgactctg
     3961 ctcggtttat ccgtacggaa atggtggaag tactgcatag tgattatgtg gaattagcaa
     4021 aagcaaaagg attgagccgt tgggaaatcg cctttaaaca tggtcttcga aatagtttga
     4081 ttccattgat gactttatta ggaccgttag ctgtagcatt gatgaccgga tcactggtgg
     4141 tagaaaatat ctttgctatt cctggtatcg gggaacaatt cgttaagtca atcacaacaa
     4201 atgattatcc tacgatcatg gctgtaacga tcctttactc atttatgctg atttttgtta
     4261 ttttgatcgt ggatctattg tacggtctag ttgatccaag aattcgtgtt tcagaaggga
     4321 gccgaggata aatggaaatg ataccaaagc gttatgaaac agttgcagat atccctgcgg
     4381 atgaatttct tccattacaa aaaaataccg aagaagaacg tgaacagatt actgcacctt
     4441 ctttgaattt tatccaagat tcatggcgcc gtttaaagaa aaacaaagct gccgttattt
     4501 caatggtttt actattggtc atcattttga tctctgtgat cacaatcttt gtttctcctc
     4561 atgatccaac tgcccaaaac gtggcttata tcaatttgcc tcctcgtatt ccgggaatca
     4621 atatcgatgg attgaatgga aaagcaatgg ttggtggtga acttgttgat aaatatgctc
     4681 aagcaaacgt cccttcaaac gtaaatttct ttttaggaac agacggtttg ggacgtgatg
     4741 tattaagccg cttatttatg ggaatgagaa tctcgttgct gatcgcattt attgctgcat
     4801 tactagatgt aacgattggt gtagcttacg ggttggtttc tggtttgtta ggcggacgtg
     4861 tcgataacgt gatgcaacgt tttttggaaa tcttatcagg aattccaaac ctcgttgtca
     4921 tgattttgat gttggtcgtt tttgaaccag gaattttttc aatcgtggct gctatggcca
     4981 tcacaaactg gataccaatg gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc
     5041 aagaatatgt tttagctgga ttgacattag gtgaatcaag aatgaaaatt gcattcaaac
     5101 atattttgcc gaatatctct agtgtcatta ttattcaaat gatgtttagt attccatctg
     5161 ctattttctt cgaagccttt ttaagcttta tcggtttagg tcttacgcct ccgtcagctt
     5221 cattaggaac gatgttgagc gaagggtaca agacattcct gtatctgcct tatctattat
     5281 ggattccggc agtaacttta tcggttatca tgattggctt taacttactt gctgatggat
     5341 tgcgtgatgc ctttgatcct aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt
     5401 gaaagactta gaaatctctt ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt
     5461 caattttcat ttgaataaag gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa
     5521 atcagtgacg accagaagca tcatgcgctt gctgtcaagc aacgccaata tagatgctgg
     5581 tcaaatcctc tttaaaggta acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat
     5641 ccgtggtaaa gaaatagcga tgattttcca agatccaatg acctctttag atccgacgat
     5701 gcccattggg aagcaagtcg ctgagtccct tagaaagcat aataaagttt caaaaaaaga
     5761 gggacaaaaa gctgcattag aattgcttaa attagttgga atacccgaag ctgagaaacg
     5821 tatcaacagt tatccgcatc agttctcagg aggtcagcgt caacggatcg tgatcgcgat
     5881 tgcactggta tgttatccgg aaatactgat tgctgatgaa cctacaacag cgcttgatgt
     5941 gactattcaa gcacagattt tggagctgct gaaagaaatt cagacaaaaa tcgatacatc
     6001 aattatcttt atcactcatg accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt
     6061 catgtatgga ggaagaatcg ttgaagttgg aacatcggaa gaaattttct acaacccgca
     6121 acatccctat acttggggac tgcttggttc aatgccgaca ttagatagtg cgaacgatcg
     6181 attgtatgct attcctggtt ctccaccgga tttattgaac ccgccaaaag gagacgcatt
     6241 ctatccaaga aatgaattcg cgatgaaaat cgatgcagaa caagaaccac cattttttga
     6301 actatcaaaa acacacaagg cagcaacttg gctacttgct ccgcaggcac ccaaagtgac
     6361 accgccagaa gaaatccaac gtcgctgggc tatctttaaa gagaaacaaa aacatactta
     6421 cggtcaagga ggaattgctg atgccaaata aactacttga agttaaagga ttgaaacagt
     6481 atttcaatgt aggaaaaaag aatgaagtac atgctgtaaa tgacatcagc tttcacattt
     6541 atgaaggaga aacattcggt ttagttggag aatctggaag cggaaaatcc actactggcc
     6601 gtacgatcat tcgcttgaat gaacctactg atggtgagat attattcgac ggtcaagata
     6661 taacgaagat caaagataaa aaaggtttga cgaaattccg gcatgatgtt cagatgatct
     6721 ttcaagatcc atacgcttcg ttgaatcctc gtatgaaagt cagagatatc attgccgaag
     6781 gaattgatgt taatggacta gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc
     6841 tattgagaac tgttggattg aatccaagcc acggtactcg ttatcctcat gaattttcag
     6901 gtgggcaacg ccagcgtatt ggtattgcac gagcattggc agtaaaaccg cgttttatca
     6961 tttgtgatga accaatttct gcactagatg tgtcgatcca agcccaagtg gtcaatttgt
     7021 tgcaagatct ccaaaaagaa catcaattga cctatctatt tattgcacat gatttgtcga
     7081 tggttaaaca tattagtgac cgaatcggtg taatgcataa cggcttgtta ttagagatgg
     7141 gaacaagtga tgaaatctat aatcacggtg ttcatcctta tacagaaagt ttgttatctg
     7201 ctattcctct accagatcca gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg
     7261 aaccagatga cggacaagta cgtcaacttc gtgaaatcgc accagaacat tttgtctatg
     7321 caactgagca ggaagtccca tattacgcaa aaaaactaaa acgacaaaag gaatctcttc
     7381 tggttgctaa ctaggaggga catagatgct aaaagctttt aaattccgta tgtatccgac
     7441 agaagaacaa aaacagcaat tgattcgtac ttttggatgt gctcgattta cctataacca
     7501 tttgctgaaa aagcggcaga aaagttggca acaaacagga gtagccaatt tttcgctcac
     7561 acccgctaca ttaaaaaaag aatacccatt cttaaaagaa gtagacagtc ttgctttagc
     7621 aaatgcacag cttaatttgg atcgggcttt tcgcaattat ttcaagggac gtgccagctt
     7681 cccaaagctt aaaacgaaaa aaagtatgtg gcaatcttat acgaccaaca accaaacccg
     7741 tacgatctat ctggaaaatg gctacttaaa gttaccaaaa caaaaagaac taatcaagat
     7801 aaatcagcat cggccagttg aaggatcgat tcgctccgct acgatttctg ctcgatataa
     7861 tgaagaattt tatgtcgcac tactttgtga tgtttcgcca gtaaaaaaag aatctttagc
     7921 taaatggatc gggatcgcct atcatccgaa gacattgatc caaacatctc gacctctaga
     7981 agtaacttta ccaaaatttc accagacaga agaaaagctg caacatgcac agcggaaatt
     8041 gaatgtgaaa gtccgctccg ctcaccatag aaagatcagg ttagatcagg caagcaatta
     8101 tcagaaacaa aagagaaaag taatggatct ttatctgaaa caaaagaacc agcgagaaga
     8161 ttatcttgaa caattatctg ggaaattggt caaacaatat gactacttat ttgtagaatc
     8221 ttttccaaaa gaagaggcac atgctgattt ttctattcac gactggcata aattaataac
     8281 aaaattgcgt tataagtccc aatggtataa taaaaaattt cttttgatca ataccgacgg
     8341 agcagaagaa agcaattccg taagaaagag ccaagtagta gaaaaaatgg gcagacattc
     8401 ggttatcaag ggataaaaaa tgaatgaaac tgataggagc gttagggtta gccttgttaa
     8461 taaaaagtct taatgacttt tgtttctagg aatttttctt ttgaagaaaa gctcactaat
     8521 tatgacaaac caataaaaca atagcagaaa aagttcgtac gagctttttc tgctattgtt
     8581 aattttttat tcagaaagtt tacgaataca tcaaaattct gaaacaaaaa acgtaactca
     8641 caaagagttg tttttgcgtt ttttcttgaa tgattaggtc tgaatctttt gctcacaacc
     8701 ttattcatgg tgccccggct tgagctgata gatttttgaa ggacggccga ttcctacagg
     8761 acgttcaccg atctcatcga aatattggag catagctttc ttaaaattgg aatgatcgat
     8821 cgttttataa tcgatcccta agaatttagc aaatactttt cgagcttccg taatcgtgaa
     8881 atctttacct aaaacttgta gaacttgcgg ctcgtgttcc attttattga ctacacggtt
     8941 gaatgctttc aagatgattt gactatggtc aaaagccaaa gtatctttgc cagaagactc
     9001 tccagtgact aaatcaagcg agatagccac ttctccgcta gataaatgga tcttgttact
     9061 atgacgttct agtgtgaacc agcgtacttc cttggcatca tctccggcga ttagcggttc
     9121 ttctccaata aaggctaagt agctgactgt gacgacccag cctcgcggat ctcgattagg
     9181 cgtactgaat gtatgcagtt gctcaatatt ttcttttgaa ataaccacac cagtttcttc
     9241 ctttgtttct cttaagacgc tttctccagt tgactcatcc ttttggacaa agccaccagg
     9301 caatgcccat gaatttcgat aaggatgccc ttttctttgg atcaacaaaa ctttcagctg
     9361 atcttcttct ttgttatagc acatcagtac gatatcgaca gtcaaagagg gcttgtcata
     9421 ttctggacgt tcctgttttt gataccagtt aagaaattct tcttcacttg ctttatgttc
     9481 ataatataat ttttcttctt ctttagaagc aaaacggttc atctgaccac ctccattgac
     9541 agtaaaagta ctttttacct tattgtagca gactctgaaa aaaaaccaag gatttacttg
     9601 aaaaatctag gttatcattt aaatatgaaa aataaatgga agtatgagat tgaataaaaa
     9661 gaaaagcaga taaaacggca atactttata ccgctcttca cgaatggcaa agaagccaca
     9721 aataacgata tatacgtcaa tgataaaacg caaaatccaa gctgctgtca taaaactgtt
     9781 caaatgccca tggaggttaa aataaccata catacgaata taaaaatagc tgaatattaa
     9841 atgtgctaat gaaagaaaag caatactaag tgtaaaaaaa agatgtttct tctgcatgat
     9901 tgttccagtc ccttactaag atagatagtc tttattatac cacgtgtgag gggagaaata
     9961 aaaagccgtt ttttagtaga tatgaaacaa aacaaaaacc cgcatttatt caacctattc
    10021 atccttttaa aggatagatt gaacgtagat agaagtcgtt tatccagtct ttttcatttt
    10081 gaaaaaaagc taacagcatg ctttttttat ggtatactag caaggaaacg aattcgcatc
    10141 atgcgataaa ggaggaagag ccccgtggca gaaaaggaaa ctttttatat cactacgcca
    10201 atctattatc caagcggcaa acttcatatc ggcaactcgt atactacgat tgcatgtgac
    10261 gcaattgctc gttataaacg gttgatgggc tttgatgtgt tttatctcac tggagtggat
    10321 gaacatggtc aaaaaatcga aacaaaagca gaagaactag gtgtaaaacc gcaagaatat
    10381 gtagataaaa tggcggctga tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat
    10441 aaattcattc gcacgacaga tgattaccat aaagcagctg ttcaaaaaat atttgatcgt
    10501 ttattagaac agggagatat ttacttaggt gaatatgaag gctggtactc tgtttctgac
    10561 gaagaattct ttacagaaac acaacttgca gaagtgtaca aagatgacga aggtaacgtg
    10621 atcggtggta aagcgccaag tggacacgaa gtagaattgg taaaagaaga atcatacttc
    10681 ttccgcatga gcaaatacgc agaccgtcta ttggaatact atgaagaaca tcccgaattc
    10741 atccagccag aatctcgtaa aaacgaaatg atcaacaact ttatcaaacc aggattagag
    10801 gatcttgcag tttcacgaac aacttttact tggggagtac cagtcaagag tgatccgaaa
    10861 catgtggtgt atgtatggat cgatgcatta tccaattata ttactgcact aggatatggc
    10921 tccgaagatg aaagtctatt cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa
    10981 gaaatcgttc gctttcatac gatctattgg ccaatcatgc tgatggccct tgatttgcct
    11041 ctacctaaaa aagtatttgg gcatggctgg ctattgatga aagacggaaa aatgtctaaa
    11101 tcaaaaggaa atgtcgttta tcctgaaatg cttgtggaac gttatggttt agatgcactc
    11161 cgttattatt tgcttcgtgc tgttccgttt gggtcagatg gtgtctttac gccagaagat
    11221 tttgtttccc gtgtcaatta tgatctggca aatgatctag ggaacttatt gaatcgaacg
    11281 attgctatga tcaacaaata ttgtgatgga ctcgttcctg actatgcctc acttgtcact
    11341 ccatttgata gtgaactgtc tacgacggct gctaacgttg tcggacgtta ccatgatgca
    11401 atggaaaaaa tggaattcaa tacagcattg gctgaaatct gggtgctgat ttctcgtgca
    11461 aacaaataca ttgatgaaac agaaccatgg gtattagcaa aagacgaaga aaagaaagct
    11521 gaattgaata gtgtcatgat ccatttagct gaatctttgc gtatcgcagc gatattactt
    11581 cagccaatca tgacagaaac gccagagaag atcttcaatc aactaggatt ggactctgaa
    11641 acaatgaatc ttgaaggatt acactttggc gaatttcctt caggaacaaa agtagttgca
    11701 aaaggtacac caatcttccc acgtttagat atggaagaag aagttgcttt tattcaagaa
    11761 aaaatgtctg aaggtactca aacaaacgaa gatacagtca aatgggatcc agaagaaaca
    11821 gaacttgttt caacgaaaga aaaacaaatc aaatttgatg tatttgaaaa agtagaacta
    11881 aaagtagcag aagtcatcaa ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc
    11941 cgactagatg ctggagacag ccaagaccgt caaatcttgt caggaatcgc tgaattttat
    12001 cctgatccaa gcgaattgat cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga
    12061 aagatgcgtg gacaaatcag tcaaggaatg atcctttctg cagaagcacc agatggctct
    12121 ttacaagtaa tcgaagcacc aaaatccatg ccaaatggct cagaaatcgc atagaaattc
    12181 aaagggggta tgacactagt tgtgtcatac cccctttttc cgaaatttga gaagcaattt
    12241 tcgtaaattc cttcttatta cttgaagcca atcatataat acagaaacag agaactatcc
    12301 aaaataaatc ctagaaaaag aagtacgagc ttcgctgatt tttgttaatt tagaataata
    12361 ctaataggaa tcgtaaaaac agaaaaaaag cataatgata aagggtcggg cagattttaa
    12421 ttgattcatc tggtactctt ttttattgtc cttacattca taaagtttga atgcaatgac
    12481 tttattacat ctatatttat atctaacagt agcgttgatg tgttaattat aagttctatc
    12541 actttatgtt caattagttt gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac
    12601 aatcatcggc tttgacttcg atgattgttt tctttatttt gtaaaaatcc tatcacttga
    12661 ttttatggta accttttatt tttgtcttca aacggcttgg taagagagca gtaagcagtg
    12721 caaatgggat cgaaaagaca taccagaaaa tcgtacgtcc gataccataa tgttgataga
    12781 gctttgtcaa catcacgtgt aacaagatca accctaaaag caaggagcca gctataaacc
    12841 aatgattatc ccaatgcatg acttcactaa gtgccatcac tccaaaagga tagaaataaa
    12901 aatagatccc gactaaggtc atccgccagt tctttgtaaa tgttccgata ttcaataaaa
    12961 agaaaatcgc tgaaagcaaa ataccgatag gcggaaaaag cccaaccaaa aaggaataca
    13021 acatccccca caagatcatc acaggacctt tgatcaatgc aacgattgcg atgaacaaag
    13081 tcagcaatat tagtttttgt gataacgttg caacagtcat taatccaaat aaaattaatg
    13141 tgaaccaatg ccaatgggac tgctgttgtt ctttacggga aaaatcgacc cggttggata
    13201 aataatctgc taatttcgga taagaacgct taaaatccat aaactgttcc tccttcgtgg
    13261 aaaaggtagg tttagtatac gaaaaataaa aaagaaatac atccgtctta agagggagtt
    13321 tttcactgaa agtggagaaa aaagaatggt aaattaaaat attctcagtt atactgaaaa
    13381 ttataccaaa tttcaaagaa gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat
    13441 caatctgttt ggcgcattag ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt
    13501 caaaactgct gctgttactg ctagtactca gtctacaagt ttgacattgc tagcttggct
    13561 tttgggtggt ttcttaacga tctgtgctgg attgacagtg gctgaattag ctacagcaat
    13621 cccggaaact ggtggagctg taaaatatat tgaagctgcg tatggaaaat tgcccagctt
    13681 tttattagga tgggcgcaaa gcttgattta ctttcctgct aatattgctg cattatcgat
    13741 tatctttgcg acacaattga cgaacttatt acaattatct accgattatc ttttgttgat
    13801 tgcagtgatc accgctgttt ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc
    13861 ttctgtacaa tcagtgacgt tgattgttaa attaatacca attgctgtga ttgtcatatg
    13921 gggattattg accccaggac aaggaactgt ccagttattt ccaatcgaag ctggaaaaga
    13981 tgttactttt gtcgaaggat tgagcagtgc gttgttagca acgttgtttg cttatgacgg
    14041 ctggttaggt gtcggtgcaa tggcagggga aatgaaacgg ccggaaaaag atttgcctaa
    14101 agcaattatt cttggtttga gttttgtaac agtcgtttat ctattgatca attttgtttt
    14161 tttgaaaact ttgccgattg atcatcttgc cggtaatttg aatgcagctt ctgaagcatc
    14221 agacgtcatt tttggaggaa tcggtggaaa gctggtcact attgggatct tgatctcggt
    14281 atacggtgca ttgaacggtt atacactaac tgggatccgc gtgccttatg cgatggcttt
    14341 agaagacgag ctacctttca gtaaacagct gacaaagctt tcaaagaagt ttacggttcc
    14401 ttatgttcct gcggttttcc agttagcagt agcttgtatt atgatgagcc ttggttcttt
    14461 cgacttttta acagacatgt tgatttttgt gatgtggcta ttcagtttac tgatttttat
    14521 cggtgtcttt attttaagaa aaaaagcgcc ggaattgcca cgtccatata aagtaccact
    14581 ttatccaatc gttccgatta ttgcgattct cggtgctatt ttcattttag gtatgacgat
    14641 gatgacgcaa acgaaattag cattgatcgg tataggtgtt accttgatag gtatcccagt
    14701 ctattatcaa aaaaagaaaa aacgctcaga agagtaaaat atttcaacaa cagacagaaa
    14761 aagtacgaac taggtcctct agatcttcgt gctttttcta tttcaataaa acaagttctt
    14821 ctattttgat ttttgctact tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag
    14881 gagagataaa catgatattt gattctcata cccatttaaa tgctgaacag ttcaatgaag
    14941 atattccaga aacgattcag cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg
    15001 gcttcgatac gccaacgatt gaaaagtctt tgcaattaag ccatgactat ccaaatatat
    15061 acagtattat cggctggcat ccgacagagg ctggcagtta tacgaaagaa atcgaaaata
    15121 aattacaaga acaattgact ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt
    15181 atcattggat ggaagacccg aaagaagtcc aagataaagt atttcgcaga cagattgcta
    15241 tcgcaaaaga aatgaaccta ccaatcagta ttcatactag agaagcactt gaagacacgt
    15301 atcaaatcct aaaagaagaa gatgttcgag atatcggtgg aatcatgcat agtttcagtg
    15361 gtgattatga atgggcaaaa cgatttcttg acttagggat gcatatttca tttagcggtg
    15421 tcgtcacttt caaaaaagca ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac
    15481 gattacttgt ggaaacagat gccccttatc tggcaccagt tccttttaga ggaaagcgaa
    15541 atgaaccagg ctatactcgt tatacagtag aaaagattgc tgaacttcgg aatcttccga
    15601 cagaagaagt ggctgcccaa acatggacaa atgcgcaccg attatttggt ctaagcgaac
    15661 atggataaga tgaagatcca agagattata gtcgttgaag gaaaagacga tacaagaagg
    15721 ctgcgggaag tagttgatgc agatacgata gaaacaatag ggtctgcaat caatgaagag
    15781 atcctgatgc aaattgaaca tgcccaagag actcgtggag tgatcgtttt taccgatcct
    15841 gatttttcag gagaaaaaat aagaaaaatc attatggaag ctgttcctca ggcaaaacat
    15901 gcttttttgc cgcgcacaca agctaaacca aagaaaaagg gaagccttgg tgtcgagcat
    15961 gcaagtgatg ctgctatttt agaagcattg aaaaaggtcg tgacgccaga catttcccaa
    16021 acggctgttt ctgaaatcac tagacaagac cttttagact atggtttaat agctggggca
    16081 caagcaaaag aaaaacgaga aaaactaggt gacgagttgc gcatcggtta cacgaacggc
    16141 aaacaattgg aaaaacgttt gaagatgttt cgtattacgc cgaaagaact cgcccaagca
    16201 atgaaaatag tggaggaaca acatgagtga atatcgtgaa attgcgacac cctctagaac
    16261 aaaagagatt ttattgaaac atggtttttc atttaaaaaa agtttaggac aaaattttct
    16321 gactgaacca aatattttaa ggaaaatcgt agaaactgcg caaatagatg aacatacgaa
    16381 cgtgatcgaa gttggaccag gaatcggtgc attgacggaa caattagcca agcatgcaaa
    16441 acaagtcgta gcatttgaga tcgatgatcg tttgatccct gtattggctg atacgatgca
    16501 accctatgac aatgtgacta ttgtacatca agatatttta aagaccgatt tgtcaacagc
    16561 tgttagagaa acatttcatg aggagcttcc gttaaaagtc gtagcgaatt taccttacta
    16621 tattacgaca ccgatcatga tgcattttct tgaatcagat ttggttgtgg atgaactagt
    16681 cgtcatgatg caaaaagaag tagctgatcg tatctctgct gaaccaggaa cgaaagctta
    16741 tggttcatta tcgattgctg ttcaatatta tatggaagcc agtttagcat tcatcgtacc
    16801 aaaaacagta tttgtccctc agccaaatgt agattcagcg attttgaaac tgacaagaag
    16861 agatataccg gcagtcgaag tgacagacga aaaagcattt ttccgattga caaaagcggc
    16921 atttcagcaa agaagaaaaa cgctttggaa taacttgcag cattcgtatg gcaaagatga
    16981 ccagacaaaa gcatggttag caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg
    17041 tggggagact ttgtcattac aagaattcgc cgcattaagc aatgcgatgt cagaaaatat
    17101 gcagtaaaaa aagaggttgt gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa
    17161 gaacagccaa acaaaaatag ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga
    17221 tgcaagcttt tgaacaccgt ttaagaaaga ggttgtgggc ctgccgttta actctaagtc
    17281 acaaggaaca atttcaaaaa acagcttctc atgtttttga gttagttggt cttattactt
    17341 gatgcaagct tttgaccact attatgccga acaaggagat gtgacacaaa ttttgtcaca
    17401 tctcctattt ttgtttcttg atcaaaaaat tcttatactt gatactcaca ctcattttgt
    17461 atggatttag aaaagaatat gtgtcttgac agttattagt aagtgagtta tactaagtcg
    17521 acatttatgt gtcaagacat attaggagag agaaaaaatg agaaaaaatt cagttcgaaa
    17581 actaacgatt tctgctttat tgattgggat gggggtcatt atcccaatgg tcatgcctaa
    17641 aatcatgatt ccaccagctt cctttacttt ggcgtctcat gtgccattat ttattgcgat
    17701 gttttttaca ccaggagttg cagtggctgt tgcgctaggt acgacatttg gtttcttttt
    17761 aacaacgccg gttatcatcg cattgcgagc attgtcacat cttgtgtttg cgttgattgg
    17821 ggcttggtac ttgcaaaagc atccgagtat cgttttaaaa aatggacagt ttacttttgc
    17881 taattggcgt ttccaagggt tcaattttgt gatcggtgtg attcattctt tagcagaaat
    17941 gctagtagtc agcatctttt actttattgg taatatgcct gaaacatatt attctcaagg
    18001 atatttttat acagtattca tcttgatggg gattggtggg cttatccaca gtttagttga
    18061 ttataatatt gcttatttta tagcaggaac actcagcaaa tattttgata tccccgtatt
    18121 tacggcagca aaaaaagaaa atcggaaaga gtcgattcaa gggtacgcga agaaaacgac
    18181 atattagaca tgatctctca aaaaaaaaac tctcatctag taatggataa taaatcctct
    18241 aagatgagag ttttttgtcc taaaaagagc tgcaccaaca tcttctggca cagctctaac
    18301 agggggcaat ttatggaagt ttgatgactt gttctgtttc tttatcaaag aagtggcctt
    18361 tgctgatgtt gaaggcaagg tcgactattt cgcctggttt atggaaatct cttgcatcta
    18421 cttttgagat gaactcagta gaaccagttt ttgtgtaaag catcgtttca gctccaagta
    18481 attcggaaac gacaacttct gatttcacta cggcttcagg tgaagcttca agggctactt
    18541 gttcactgtg gatgtcttct ggacgaatac cgaagataac ttcttttcct tcataacctt
    18601 tttcaactaa tactttgttt tttccttcag gaatacgaag cttaagccca tgtccatctg
    18661 tgataacacc atttttcaat gtcacattaa agaagttcat ggcaggagat ccaataaagc
    18721 cggcaacgaa catattgacg ggtgtatcat aaacttcttt cggtgtaccg atttgttgga
    18781 taaatccgtc tttcatgata acgattcggt cagccatagt cattgcttct gtctgatcgt
    18841 gagtaacata gatggtcgtt gtttcaagac gctggtgaag tttggcaatc tcagcacgca
    18901 tggctacacg taatttagca tctaaatttg ataatggctc atccattaag aagacttttg
    18961 catcccgaac gattgcccgt cccaatgcca cacgttggcg ttgtccacca gataaagcag
    19021 ctggttttcg tttcaagtat tctgtcagac ctaagatctc tgctgcattg tccacacgtt
    19081 ttttgatgtc tgctttatca tatttacgta atttcaagcc aaaagccata ttatcaaaaa
    19141 cagtcatatg aggatacaat gcgtagtttt ggaaaaccat ggcaatatca cggtctttag
    19201 gtgccacgtc attcatgact tttccgccga tatttagttc tccttcagag atatcttcca
    19261 gtcccgcgat cattcgcaaa gtagttgact ttccacaacc ggaaggacca acaaaaacga
    19321 taaattcacg gtctttgatt gatagattaa aatcagttac agaataattt tctgcattgt
    19381 catatttttt atatacgttt ttcagagcca tttctaccat aatacgttcc acactttctt
    19441 ttttagtttt attaactatt taaagtataa atgaaagcgt taactaactt caatgtcatg
    19501 ttgaacaaga attttttctt ttttttgtca atgtgctagt tttaagcttt aataaatatc
    19561 cataagaaat aaaaactgat acactggagt aaataagtat gttaacaata tcaaaaggag
    19621 tgtctcttat gaaaatcgct ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat
    19681 tagcaacagc gtataaaaca atattggaaa aacatgaact ttttgctacc gggacgactg
    19741 ggcagaggat cacggaagca accggcttgc cgatccatcg gtttaaatcc ggaccgttag
    19801 gcggggatca gcaaattgga gccatgattt cggaggatca gttagatttg gtgatttttc
    19861 ttcgagaccc gctggctgca cagccccatg aaccagatgt aacagcactt atccgtttat
    19921 ctgatgtcta tgagattccg ttagctacga atatcggaac agctgaggtg ctactaagag
    19981 gactggaagc cggcttccta gattttcgaa gaatcgtcca tgaaatagac aagcatccac
    20041 tttcattttg atggataagc gtatggaaaa ataacgttta atgttcgcct atatgtagct
    20101 ataaaagcta aacaaactga taatatgcga tataatacga acgtattgga taatataaac
    20161 aataatatta gtttttcgta tttgcagtaa atgtgaaaat atgctatagt ttctgtggga
    20221 aaagatccca aaaataatac agagtagaaa ataaagcgtt aagtgctaag aggatgggat
    20281 gttgcctctt ggacgaagga cgtaatgttc gcggttttat tttcgcattc gctgcatgat
    20341 acgatgtagc agctcttaaa ggagatgcta aaaatgaaga aacaccggtt agacgcacgt
    20401 atgattgcta ttatgggctt attgattgca ttgatggtca ctttgtcccg actggtagca
    20461 atcgagaccc catttattaa aatcagtgtc acatttattc cacaagtgat catgggcatt
    20521 ctttttggtc ctttttggtc agggataggc gcagttttag ctgatttagt aggaatggcc
    20581 ctcttctcga aatctgcatt ctttattgga tttacattga atgcatttat tgaaggagca
    20641 atctacggat tctttttcta tcgaaaagag atcacttgga aaaatgccat tctggctacg
    20701 cttagtgtca cgctgatcat caacttattt ttgacaccat tatggcttgc gttgatgtac
    20761 catgtgccat tgttcagctg ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg
    20821 ttaccgattc aatcaattgc catttattac gtaggacgta gtattcctta caaaaaaatt
    20881 ttaagaagtt tagcgatcca cgctaaataa aaaacaagca gtcttcccat cccagaagac
    20941 tgcttgtttt tattgtgctg tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt
    21001 ttgaagcagc tcatcatggg tgcccatttc gacaatagct ccctgatcca tcactaatat
    21061 ctggtccgca tttttgattg tggacaggcg atgggcaatc acaaaactgg tttttccttc
    21121 catcattgct aagaaggctt gttggatttt tttctctgtc aatgtatcga ctgagctggt
    21181 agcttcatct aagatcaaca tagggggatt actgatcatc gttcgagcaa tcgtcaacag
    21241 ttggcgttgt ccatctgata tctttaaccc ttgaccgcca atcttagtat ctaatttttc
    21301 cggcaaacgt tctacaaatt catacatata agaggctttt agggcatcat tgatttctgc
    21361 gtcacttgct tccggattcc caaaacgaag attttctcgc aatgtactgt caaatagcca
    21421 agtatcttgt aacaccattc caaaactttt acgtaatgta tcacgttgga tagagcggat
    21481 gtcatggtca tcgactgtaa ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt
    21541 aacaagtgtg gattttccgg cccctgtttt tcctactata gcaatggttt gacctggata
    21601 agcagtaaaa ttgaaatctt cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg
    21661 atcaaaagag acttttcctt tgatattttt gggttccaag gcataggcat ctggcaaatc
    21721 aggcttttct tctggctgat cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt
    21781 ttgcagttgt gtcgtgattc cagaaagttc gataaatggt ttagaaaact gacttgagta
    21841 gatcgtaaag ctggaaatca caccaacagt gattgacgaa ccactcttca atgccaatag
    21901 gccgccaact aaaccaatcg ataaataagc aaggtgatct acaaaacgag aagacgggtt
    21961 tgtcaaggaa gaagaaaatt gtgcccgttg tcctttttca tatagatcag catttagcag
    22021 ttcaaattgc tgttgactag cttcttcttg ctgaaaagct ttcacgattt tctggttacc
    22081 gattcgttcg gtcacaaagc ccgagatatc cccaataatc ctttgctgtc tatcgaaatt
    22141 tgtttgtgaa gcacgagcaa cgatccaact cacaaggaaa ataattggtg tagaacaaag
    22201 aacgaccaat gtcagaagtg gacttagacg catcataaaa ataaacgaaa gaatgatgat
    22261 tgccactcca gaaaataatt gattgaaggc cgcagaaatt gcaatggaaa tattgtccat
    22321 atcatttgta aatcgactga cgatatttcc gtgggagttt tgatcataat aacgaagtgg
    22381 caatcgattc agatgagcaa atgcatcttt tcttaatttt gcaacggata agtaagctaa
    22441 ccgattgctt aacacttgga tgagccattg actgactacg ttgattaaaa cgataccacc
    22501 gaatagaata agtaaatgga acagtcgatt gaagcggacc tgtccttctc caatcatttg
    22561 atcgatactt tgaccaattt gtaacgtcat cacaactgtt gcaatcccat tgatgattcc
    22621 tagtatcaca gctagccaaa tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg
    22681 gagcgtctgc aaggataatt tttttacttt cataactatt cttcctcctg tgatgcgacg
    22741 atttcctgat attctgctga ttgagcaagt aattcttcat gggtaccctg agcagcaatc
    22801 ttaccttgat ccatcactaa gatatgatgt gcttcttgaa tagagcggac acgttgggag
    22861 ataataatca ctgtactgcc aagataagtc tgtagcgcct tacgcaaatc caagtctgtt
    22921 tgataatcca acgcacttaa agagtcatct aaaatcagta atgctggctt ggcaatcaaa
    22981 gctcttgcaa tcgtcagtcg ctgtttttgt ccgccagaga aatttttccc attttccatg
    23041 acttttgtat ctaaaccatt tggaagctga cggacaaatt cttctgcctg agcgattttc
    23101 agagcttccc aacattcttt ttctgtggcg tctttcttac cccattgaag gttttcacga
    23161 atagtcccgg taaatagtac agaagtttgg ggaacaagag caatttgctg acggagatga
    23221 tccagctgcc aagaacgaac atccattccg tccattataa ctgtaccgga actgacatca
    23281 tagaaacgcg gaatcaattg tgtcaaagtc gttttcccgc ttccggttgg accaatgatt
    23341 ccaaggactg tatttcgctt cagctgaaat gtaatatttt ggagagaaag accagaatca
    23401 ggagtataac ggaagtcaac atggtcaaaa gcgataattg cttcagaaga aaaatcggga
    23461 gtagtactga cctcagcttc aagaatcgaa ttttctgtgt ctagcacctc tttcacccga
    23521 tttcctgaag cttcggcacg tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc
    23581 aaaagcattt gattcatata attgatcaaa gcaagaactt gtccttgttg caagctacca
    23641 atatttactt tgatcccgcc aactgtcagc aaacagatga tcccgacatt catgatcaag
    23701 gttgtagcag gtgataatag ggcagagata ttggaaacac gtaaataatt gttcgctaga
    23761 tcatttgtgc ttttatcgaa cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg
    23821 acacgaacac cgctaagatt ctgactaacg tgttcattga gctgatctag tttttcttgc
    23881 actttttggt agagaggaac ggtttttttg atgataaaaa ataaaatgat tgagaaaata
    23941 ggcaataatg ctaagaaaaa cagacctact tcccagtcga tcacaaaagc catgaccact
    24001 gacccgatac ttaaaaacgg tgctctgatc aataaacgaa tgaccattgc taaagctagc
    24061 tgaagctgat tgatgtcgtt tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc
    24121 aactcgttgt gagagaaagt attgattttt ttgattagct gattacgtaa ctctgtacca
    24181 aatccttgtg aagctactga agcatagtat tggcaaatga tagcacaaat cagaccaatc
    24241 acagacatag cgaccatcca taagcccatt tcaatgattt ttcctcggtc attcattttt
    24301 agtccattat cgatcaagga agccatcatt aaaggcagga caagttcgaa gacagcttca
    24361 agaaatttga agaacgggcc caaaatgatt tgcttacgat agtcttttgc ataacgtaat
    24421 aactgaatca tatgattcct cctattattt atttgtccac aattgaaaaa acaagtacta
    24481 ccacattgta gcttaggaaa tttagaatga aaagagaaca agccttttca taaatgaaag
    24541 ttttattatg aagaagcttt agaaattgaa tagactattt tttttaaaaa aaagaaaatg
    24601 agaaaacatg ccagtctctt cattcatagg aacgtctatt ttacttaatt tttctataaa
    24661 tgaattctgc ataaaaaata tatcaagtaa atgtttatag atgaaactct atttacttct
    24721 tttttaattt gttcttgcaa tcttttttgc tacgtaattt ttgtaaaaaa gtgggttgag
    24781 agaaatctaa ttgattttca aagaaggagt atgaaaatga gaaaaatatt tattcgttta
    24841 ttgtgggtac tatcttagag gaataaataa tagtggagaa aactatggtt tggcgcagat
    24901 tctatcaggt ttgatggaac gaagacaacc tcccgacatc aagtcaaaag ctacttgaga
    24961 ttttgatctg aaaataaaaa aagaaaacaa ggtaggaaca tcaatgtttc taccttgttt
    25021 tcgtaatcaa tgggaaaaaa ctaagagtgg tgtacgtaat caataaaaag gatggtgaca
    25081 acagtacctc tttactctct gaaagcatca taactcgttt ttcctcatta tgccaataaa
    25141 ttgttgttat accacatata aggatttgta atagctaaaa acagacagga aagttgaaat
    25201 ctgtgaatct tattttgtat ggaaggattt atagaacgca aaaatcaaga taaagatgaa
    25261 taaaaatcct gtttcggcta tatattgcgt atctacgcca ataaaacgag tttgttctgt
    25321 catagccata taaagcaaag gcaaaaatac aactacagcg agataagatc cgatgatttc
    25381 tcgaaaagtt ttccaggaag tatgtttcct gtttttatat ttatccgatt tcattgaata
    25441 gtcatagcct ccttattctt tcatatgttg aaattccagt ataaaaactg gaaggaactg
    25501 tacgtttttt agaataaaaa ttataaggga taagtaggct gctgtctata ataagatata
    25561 tttttcaatt ctttattaaa gattttattg acaaaatgaa tccgttttct atgatggagg
    25621 aggggtgaat aagttgttga agaaggaaat tttatgttac ttagagaacc agacagcctt
    25681 tttcgattta gaacatatga acgaggtctt tacagcacaa aggttagctg atcattttgg
    25741 ggtaaaaaga aacacagtca gtcattattt gaaccagtta aatgatgagc aactactagt
    25801 caaaatcaat tctcgaccag tgatttattt ccacaaagaa gcttttgaaa aacaattttt
    25861 taagttacgc acgaattttt atttttctat cgatgatctc aaagaggaac agcctttttt
    25921 cgcgcaacca aaagacttat tctcttttat gatcggatac gacgccagtt tgaaagaaag
    25981 tatcgaacaa atcaaaacag ctctttacta tccagatggc ggattaccac ttttgattac
    26041 aggcgaaagt ggcacaggta aaagttattt agttaatttg gtctatcagt attgtctgct
    26101 tcatgatctg ctagaagatt ctgcaccatt catcactttt aattgtgcac aatatgcaga
    26161 taatcctgaa cttttgacta gcaatttgtt tggacatgtt aagggagcgt acactggtgc
    26221 agaagaaaat aaaaaagggg cttttgaagc agcagatggt gggatcttgt ttttagacga
    26281 ggtacatcgc ttaacaccag aaggtcagga aaaactgttc acctatcttg atcaaggtgt
    26341 gatttatcgt atgggagaac ctaatttgtc acgccggatc aaaacacgtc tcttttttgc
    26401 tacgactgaa gagattacta gtcatttctt aacaactttc attcgaagaa tacctatccg
    26461 aatcgaacta cctgcactta atcagcgtag tcgtaatgaa cggctagaac tgatttactc
    26521 tttttttctc gaagaacaaa gaaaaatggg gagccctttg tcagtcagtg gaccagcttt
    26581 gtcactttta acaagtcaga aattatccgg aaacatagga gagctgaaaa atatcgtcaa
    26641 agtttcagca gcaaaagcat atgctgaaca gcgtgaacag cctgaaatcc atgtcacgat
    26701 ccatcatttg caaaaagaac tacttgcgca gcctattact aaaaatacag cacagcaagg
    26761 aatctatatt acgcaagacc agaccttaga gcaattaatg gagaagcaac agcccgaaca
    26821 gcagcgaatc gttaaaagtt ttgagcaaat cctaattgat ttcaagaaaa ataactgtct
    26881 gctatattct tgcgaaggaa aattaaagca agaagtgatt caattatttg attttttgct
    26941 gtttgagacc agtagacaag aaaaacacga gctgctggtt tattggacac aatctatccg
    27001 agagaccttt cgacaaatgg aaactgccta tcaaatcaaa ttcgatggaa acagtgtcta
    27061 tgccttgagt tactatctct atcagcgaag cactatcaaa tggttaccgg aagaacatga
    27121 tattatccaa ctaatcaaag aattagaaca acaagtttct cagtcttatc catctagtta
    27181 tcactatgtc caacgaattc tggaattaag taagatcaaa ttagatattg aagacacaag
    27241 tatggatcgg attttactga cgatctatct aaaaaaagcg aaatggtcaa aagaaaaacg
    27301 actgccaaaa gcgatgattg ctgctcacgg ttatgcaact gctagcagta tggcaaatgt
    27361 agtaaatcgt ttattacaag aggatctatt tgaatcattt gatatgccac tagatgtcac
    27421 accacagcag attgcttcag aaatccttga ttattgcgaa aacagcgatg tttcaaatgg
    27481 attgatcata ttagtagaca tgggatctct gaaagaaatc catcagtttt ttaaaaaaca
    27541 gttatccgta ccgcttttga ttttgaataa tgtgacgaca cctttagcaa tcacagtagg
    27601 ggaatgcctg caaaaaaatg tatcattaga agagatagca gaagagactg ttcgacagat
    27661 ccagccagaa tggcgactgc tctatccaga ggaaaacaaa cccaaagcac tcattactac
    27721 ttgcttcact ggaatcggta cggcggtcca cttaagtgaa ctgctagaaa aaagcttacc
    27781 tacggcttgt cagttgaaaa tcatcccata tgaatatcag caattaaaag ataagaaaaa
    27841 cagtgaccca ttattttcca tatacgaagt cgtaggaatg attggcacca cagatcctga
    27901 tatcaccaat atcgactatc tttcattaga agacctcatc tcgggggaaa agatgtctgt
    27961 actagctgaa tggttggaca ctactatgaa tgcttcagag aaggaaatat tcaatcagcg
    28021 catcattcgt aatttttcat tggaaaaagt actggattcc gtgactattc tagatacaga
    28081 gaaggtgatg ggagaaatcg atcattttat gagagaatta gaggtgcagt tgaatcgaag
    28141 catcagcaat cccaaaaaat tagcgttgta tgtccatgtg agctgtctca ttgaacgatt
    28201 gatccgacaa atgccaatcg aaacctatca aggattagag aaatggaagc agtgtcaaaa
    28261 agaagggtta tctgctatta aatctgcgtt tagtgtcatt gaagagcatt atagtgtcat
    28321 gatccccaat tctgagattg cctatatcta tgatattttg agtgaagata cagagtttct
    28381 ctcgatagaa gaagaatttt gaacataagc aagttgttgc cactaatttt ggcatgcaac
    28441 ttgctttttt agtatgtgta agtgaaaaga ggagatgaaa aagtggatag agaaattatc
    28501 ttagcttccc atggaaaatt tgcttcaggg atattagatt cattagagct gatctatggg
    28561 aaaaatcatt ctattactgt attcgattgc tatactgatg agaattttga tttagcggaa
    28621 acagtaaggc agctttttat gaggtacaaa gataaagaaa tcattgttct gacggatctt
    28681 tttggcggaa gtgtcaacaa cgaattctta caatatatca atcgggatca tgtctatctc
    28741 gttgccggat tgaatttgcc actattgatt gagctagtat ctcggttgga cgctgaagta
    28801 gagacaactg ttttgatcca gcaggtgcta actgattcaa aaaaaatgat ccagttttgt
    28861 aacgagcggc tcaatcagaa aatagaagaa gacgaatttt aggaggaaga aaaatgattg
    28921 tattgacaag agtcgaccat cggttattac acggacaagt agctttttca tggacacaaa
    28981 caataggcgc agactgtatt ttgattgcta atgatgatgt acctacgaat gagatacgaa
    29041 agacaacgat caaattagcc aagccacaag gagtcaaact agtgatcaaa tcaattgatg
    29101 actcgattgc cgcgttgaaa agcggggtga cagataaata taagttattc atcgtagtgg
    29161 aatcgatcga ggatgcatat aagctggcag aagcgtatcc agaaatcaaa accatcaact
    29221 taggagggat caaggcaaaa cctggtactc gcagcatcgg aaaagcggta aatattttac
    29281 cagaagagga ggaacgcttg aaacgattaa ttgacaaaca aatagaagta gaaatccgtc
    29341 aagtaccagc agataaaaaa gtggatgtaa gagaagtttt gtaagaaagg aagaacctca
    29401 atgattgtac aagcagtctt attaggaatt gttgcatttg tcgcacaatc agaatatgca
    29461 ctagggacat cgttgctttc tcgtccgatt gtgacggggc tgtttacagg aatcgtgtta
    29521 ggtgatgtaa aaactgggat cattatgggc gcaactttag agttagcatt tatcggatcg
    29581 ttctcagtag gtgcttcgat tccacctgat gttgtgaccg gtgggatttt agggacggct
    29641 tttgctatta cagctggagc cggtacagaa accgcgcttt tactaggttt gccgattgct
    29701 acgttgacat tgatattgaa aaatatttac ttagggcttt tgattccaat tatgaaccat
    29761 aaagcagaca attatgcaga agaaggaaat tataaaggag tcgagcatat gcacttgctt
    29821 gccggtatcg gtctttcctt gatgcttggt gttgtggtaa tggtttctta tatggtggga
    29881 agcaatacga tcggaaatct attaaatatg attcctgact tcgttcaaaa ggggttgtca
    29941 gtagctacag ggttgattcc agctcttggt tttgcgatgc ttgctcgttt gctgatcaac
    30001 aaacaagttg caccatattt tttcttaggt tttgctatag cttcttactt agagattcct
    30061 gtgaccggag tagctatttt tggtgcgatt ttagcagttg tggtagtcaa tattatgaat
    30121 gtccgtcaac taccacttca aacaactgca ggggaggtag aagaagatga agaattctga
    30181 gatcaaaaac agcaaaataa cgaaaaaaga gctaaatcac gtattttggc gttcttttca
    30241 aatggagttt tcttggaatt atgaacgtca gatgaatatg gcttatgttt atgcaatgat
    30301 tccgattttg aaaaagttat atcagtcgaa agaagaaatg gcagcagctt tgaaacggca
    30361 tctagagttt tttaacacga ccccacatat cgttacactg attttaggaa tcacggctgc
    30421 aatggaggaa gaaaataagg atgatcctga atttgatgtg actgcgatcg atagtatcaa
    30481 aacatctttg atgggacctc ttgcagggtt aggagattct ttcttttggg gaactttgcg
    30541 attgattgcc acaggtgtgg gtacctcttt agccatgcaa ggaaatattt taggccctat
    30601 cttgttcatt ttgattttca atattccgca cattctgttt cgctatctag caacaggttg
    30661 gggatatcgc ttaggaacgg gttttttgaa aaagatccag gcaaacggta tgatggaaag
    30721 tttgacatta ggcgcgtcta ttattggact gatggtcgtt ggagcaatga ctgccacgat
    30781 gatcaccatc aatattcctt taaaaatcgg ttcaggagaa aatgctgtga ctgttcaaag
    30841 tatttttgat gatatcgttc caaatatctt atgtcttgga gcatttggca tagtgtttta
    30901 tctattgaag aaagaagtga aaccattaac gatcttgatc ggactagctt tctttggaat
    30961 ttttggttcg tggataggga ttttttaaaa aaggagcaga gaaaatgttt acgatgcaag
    31021 attatatcta tgaagaaaag gaagtactaa gtacgatctt gaaaaagaat gatttttcga
    31081 caagagaaca tatgaaaaaa acagtgaatt tactgatctt agcaacgggt tcatcttata
    31141 acgcttgttt agcagcgaag ccagcattgg aatcttatgg tgatcttact gtcgatattc
    31201 aagaaccttt ttattttttc aattatggaa aactttctcc ttcaatcgat actgtgattg
    31261 ctgtgtcaca aagcgggaaa agtgcttcta ctgttgaagc agtaaaaatg atacaaaaac
    31321 aagggttacc tgttgttgct atcacaaatg acgtgcaaag tccacttgct ttagaagctg
    31381 cacaaattat tgatttaggt gccggggtag aatcagtagg cttcgttaca aaaggctatt
    31441 ctgcgactgt tttacagtta ctactcttag ggataggaat tggaataagt aaaaataaaa
    31501 tcagcaaaaa aatagaacaa gactacatgc aacagttgcg caaaataatc aaccatttac
    31561 cagcgattat tcaaaaaacg gaagaatttt ttgatggata tcaaagttta tttcgtttag
    31621 cacagcgttt cgtcaccatt ggatatggtc caaactgggg aacagctaaa gaagcagaga
    31681 cgaaattgac tgaaacaatc cgcgttccgt cacaaggatt tgagttagaa gcttatatgc
    31741 acggcccata cctggaggca gatgcttcgc atttgctatt ctttattgaa ggggaaagtg
    31801 taaataagga acgttcgcaa aagttgcaaa gatatatgag tcggtacgtt ggagagactc
    31861 tcaccattac cacaaaaaaa gcaagaaacg agaaaacact tggattagca atagaatgtg
    31921 atgaatatct gtctgtatta gcgcttgtcg ttccgtttca actattcgct tataaaaccg
    31981 cagtagctaa aggtattgac ttgaacaaaa agatttttga agactttgat acagtcttga
    32041 aaagtaaatt ataagaggtg gaaatcatgc tgaaattcaa tgaagaagaa caaatcaaag
    32101 caataaaagg cgcactagct ttacgtccgc aagtagaaga aatcatcgat aaactttatg
    32161 cggaaaaatt tgacgctgtc tattatctag gaatcggtgg aacgtatgct tcctcgatgc
    32221 aagctgtcac ttatatgaat gggaaaagca acttgcctgt ctttgtacag catgctgcag
    32281 aatattacac tactggaaac aaaagattaa caaaagattc tatcgttatt ttgtcctctg
    32341 ttaccggtac aactcaagaa gtagtgaaag cagtagaaca aatcaagaaa gtaggagcga
    32401 cactgattgg atttatcgat aaagcgaaca gtaagttgtc tcagctatgc gattttgtgg
    32461 tcacttatcc agctcctgga acagaacaaa tcaaattttt tatggcagca gatcgattga
    32521 tgtatttgaa tggagaattc gaagcttatt cagaatacta tgaacagctc gaacgttatt
    32581 taccaacagg gttagtggaa gcagagaaaa aagcagatgc atttggctta tcatttgcag
    32641 aaaaacatcg acgtgatagt atgcattatt ttattggagc agggaatcaa tggggagctg
    32701 tttattcata tgccatgtgt tactgggaag aacaaagctg gcttccatcg aaatctatcc
    32761 atgctgccga gtttcttcac gggacgttag agatcgtaga agaaacaacc ccagtcacat
    32821 tatttttagg cgaagatgaa cagagagtgt tatctgaacg tgtggctaaa cttttgccaa
    32881 aaatttgcag caattataca ttgatagaca caaaagacta tccagtagaa ggtatcagtg
    32941 aaaaatatcg cggacgtgtt ttgtctttct tattgatgca tgtagtgaca caacgcatcg
    33001 atgctcatgt ggagcagttg aactgccatc cattagagat acgccgctac tatcgtcagt
    33061 ttgattatta ggatagactg gataaaaaga atataaaaaa taaagagagc agcgataact
    33121 gctcccttta tttttttaaa atattggcat caattacgtt ataaaacaca ttttttgcat
    33181 tatctacagt tcaacttata atctgtttaa tgggacatgt gggtgccaac cattttttgt
    33241 tatgcaatag tactatttag ttgttgcatg gatccgtagt aaaagtccaa gtacaacgag
    33301 ttgcgccagt ttacatagcg ttttgtatcc agaatgttga ctaaaacaaa agttcctatg
    33361 tcaaccgtca acagagacaa tctgtccgtt gcaggacctg acagatttag aaatgttcaa
    33421 tctgtctctt atgtatttta aatcaatact cactaggaga agtttcctaa cctcctaata
    33481 gtaatgctgg tagtaacatt gtcaaaactg cttcctctta ggaacaagca aagcataacg
    33541 agtgtaaaaa taaaatgtag actattgtaa catatcattg attatttcaa gtaaaaacga
    33601 taaaagaagt aaacaaacac ttaaaaacaa tggaaattag tcccaacttt aagaaatgta
    33661 tactataatg gaaattatca attatttatt tggaggaata tccatgaaac aaaaaatcgc
    33721 agttcttggt cctggatctt ggggcactgc tttagcacaa acattggcag aaaacgggca
    33781 tgatgtccgt atttggggaa atgtacctga acagattgat gaaatcaata agtatcatac
    33841 aaatcagcat tttttacctg atttgacgat cccagaatcg attatcggat ataaagaatt
    33901 ggcagaagca gtggataacg cagatgctat tttatttgtt gttccaacaa aagcgattcg
    33961 ttcagtcgca caggaactta ttggtaaaat gaatactaag ccaatcatta tacatgcaag
    34021 taaaggacta gaacaagata cgcataaacg aatctcagaa gttttggctg aagaaattcc
    34081 agaagaaaaa agacaggcaa ttgttgtatt atctggtcct agccatgctg aggaagtggc
    34141 agtacatgat atcacaacaa ttacagctgc aagtattgat caaaaagcag cagcctatgt
    34201 acaaaaactg ttcatgaatg attatttcag gatttataca aataatgatg tgattggcgt
    34261 agaaactggt gcagcactga aaaatatcat tgcaattggt gccggcgcta tccatggatt
    34321 aggattcgga gatgatgcaa aagcagcaat catgactcga ggattggcag aaatcagccg
    34381 tcttggtgtc gcgatgggtg ccaatcctct gacatttatc ggtttgagtg gtgtggggga
    34441 cttgatcgtt acttgtacaa gtgttcattc tcgcaattgg cgtgccggta acttgttagg
    34501 acagggacat aagctggaag aagttctaga aaacatggga atggtcgttg agggtgtggc
    34561 aacgacaaaa gcagcagtag agctagctca acagctaggt gttgagatgc caattactca
    34621 aacaatctat aatgttttgt ataatggaga agatatcaaa aaagcagcaa aagaaatcat
    34681 gttacgtgac ggaaaaatgg agaatgaatt ttctcttcgt taaatagatg atcaatagtt
    34741 aatcgtcgtt taggaggaac agcaagtgaa ggtcaaaaaa gcagtcattc ctgccgctgg
    34801 gctaggaaca cgttttttgc cggcaacaaa agccatggca aaagaaatgc ttccaatcgt
    34861 ggataaacca acgatccagt ttattgtgga agaagcattg gattcgggaa tcgaagacat
    34921 cttgatcgta acgggaaaag aaaaaagacc cattgaagat cactttgatg caaatatcga
    34981 attggaaaac aatttgcggg aaaaggaaaa aacagaatta ctcgctttag ttgaagaaac
    35041 gactcaagtc aatcttcact tcatccgtca atctcatcca agaggactgg gggatgccgt
    35101 gcttcaagca aaagcattta taggaaatga accgtttgtc gtcatgctgg gggatgattt
    35161 gatgaaagat gatatcccat tgactcaaca attgatgatg gattatgaag aaacacaagc
    35221 ttccgctgtt gccgtcatgc gtgtaccaga aaatgaaaca tctaaatacg gaattattga
    35281 tcctaaaaca gatagcgaaa agggtcgttg cagagtaaaa gggtttgtag aaaaacctga
    35341 aatgggaaaa gcaccaagta atttagccat tatcggacgt tatctactga ctcctaaaat
    35401 atttgagata ttagaaactc aagaaccagg agcaggaaac gagatacagc taacggatgc
    35461 acttcagact cttaaccaga cagaggcggt ctatgcgaga gagtttaaag gaaaaagata
    35521 cgacgtaggc gataagttag gatatatgaa gacaaatatt gaatatggac ttcaacattc
    35581 ggaaattggg acttcattaa gtgcatatat tatccagttg agtaaaaagc taagaacaga
    35641 aagttaactg gaaataagtg gcctgctgag aatgaaaggc tatttgttat taaagcggca
    35701 gcttgtgcgt catttgcgca actgtcgttt tagttttttg actctagatc agttgagtcg
    35761 tggaaaacga aagatagaaa aagactttcg tgaatagatt cagaaaatat tatgctaatg
    35821 gtttaatttt tttttaagtt gtataaaaga aaatgattac ataagagaaa tgaaagccaa
    35881 gccagaagat tcatagtaga ataaaaaggc gaatttttta attataaaaa aaggtgtgtg
    35941 ttcactatgg gaaaattagt cgctgttgca cagtcgctgc ttgatatact caagtggctg
    36001 atggaaggaa gcaattggat atttgtgttg ccactattgc ttattagtct atttatcatg
    36061 tttcgcatgg tcagaaaaaa agaaacgttt ctgattttta tctggtatgc agcatccgtt
    36121 ggggttgcta ccttgatggt tgaattggcg ttaggtagtt gggaagcttg cgtatttgct
    36181 ttattttttg ctgtactgta tctaatcatt tatatcccat atatgaaaag aaagtctcgg
    36241 ataggcagaa aaggtagaca tacaactact ttttctagta cagattctct cgaacaaagt
    36301 agaagaacga gaagaagacg gtaatattta gtaggtatac gcaaaaaaag ataaaataat
    36361 ggataaacta ttgagatgag agatttattt aattggtgga aaaaagcttt tttcacttat
    36421 ataaaagaat cttttttaaa ggaatgcttg ttgtatcggg ataagccatt ccttttttta
    36481 tgttatttat atatatataa atcataccaa tgtttttttg tattttcttg cttgcacttg
    36541 gataagagat tacttacaat aaattcagat agaaacagct gaataaaaaa aggctccctg
    36601 acccttagtt ggcgctaaga atcagggaat gaccagtcac acacatacac cctgaccgat
    36661 cattttgcca tagtcaatgc agtgcatcga cctaatttat tgtacctaat ctagggtgaa
    36721 aattctaccc ctatgtagat attttccatg gtctgcacgt tgttttattt gctatgtcaa
    36781 ggtattgggc gtattcgttc aatgcctttt ttgtgtttga aacagcaaag accacatgtg
    36841 ttactgtcta actactgttt ttttatgtac ttccttagat taggcagctg tttctattca
    36901 tgtcaagtat agctaggcgg gagagataaa tgagtttatg ttattttgac tattatcacc
    36961 aattggaaga gaaattaagg atagaagagc cgctgatgct tcaagctggc acttggatcg
    37021 cgaaacaaat taatcaaggt ggtagattgc agatatatgc tagccgcaca ctaagtggtg
    37081 tagcatttga attttgggat caaaccccgg atcttgtacc aagtatattg attgaaaatc
    37141 cagccggagg tgtctatgaa acgctagaag gtgcaggaca ggcaattatt gatcagcttt
    37201 ctgtcaaatc agaagacatt tttcttcttc tttcgaatga aggaagaaac ccggcaatca
    37261 tcgaactagc tcagtggatc aaagaaaatg gtcatccatt aatcattgtt actggatttg
    37321 atttgtcacg aagtatcaaa tcgcaacata gcagtggttt gcgattattt gaatttgctg
    37381 atttggtttt ggacaaccac gccaatttac atgatgcagt cttgactttg ccaaatctag
    37441 atccagcgat ctgcggaaca gcctctcttg caacgatctt tttgctgcag caagttttgt
    37501 attttacagc aaaacagcta gtccaggaca caagtggaaa atagttatcc acatgttgac
    37561 aaagttcctt aaaaaaataa tgttccacat aaaaatggag cgtttgaaaa aaagaaaacg
    37621 attattaatg aggattaaca acgactactt tttttgtttt agttatttta agatggatca
    37681 tctttgattt taagcttgtt ttgtttaaag tttgttattt ttataagttg tttcacagtt
    37741 atgagaaatg agttataaga atatgtagtg tcagaaaaat aaaaagtagt ggaaaaagag
    37801 acaagaaaga atccatatgg attctttctt gtctcttaat acacgttgat gaaagagtct
    37861 tccccaaaac tctttacaaa gtaattatag tacatttgtt atcattattg caatgaagtt
    37921 tacctgatat aacaaaattg tccaacagga tacctttttc taatagaaat aaaatacaca
    37981 aaaattgaag gatagataat ggatatttat aagtgtttat tatttttttt gcaatgaaat
    38041 tcaatttttc aatgattgta aaatgaaaaa aacaggacga gattttctct aatctaaaaa
    38101 taaaaaaaga attttaagga aaaacagaga aataaaatac ttaatataaa tggaaagaga
    38161 aaaaaataat catacatagg agaaaaaaaa caaaatagaa aaaagtgatt ttctaataaa
    38221 ttattcatgt ggaaatgtca tttttactgt ataattcatt tgattatcca ttttttattc
    38281 atcaaaatta ctgaaaaaag aaaggaagaa ataaatttga agaaaacaaa ttcttcagga
    38341 aaaccgaaac gcgacttcac taaattatcg acaccccata cgtatgtcat tatttttggt
    38401 gtagtcatct ttgcatggat actgactttt gtcgttccag caggaaagtt cagcacgcaa
    38461 gatattgaat acaaagatgc aaatggcgaa acaagtactc gtactgtttt acgtcaagat
    38521 tccttccgtt atgcttacga gctggataag tcgtatgtgt ttgatcaatt agaagaactg
    38581 caagatcatc cagcagaacg tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc
    38641 attgctgacg gagaaaaaaa tcttacgcag gaaaaactag atgaaatctc cttgacagat
    38701 gatgtgcttt acgatcagta cggcgaaaat atctatgata cttctaaaaa attgcataaa
    38761 acagccaaaa tatggggaac agatgatttt ggcggctttg gtttcttaaa ttttgttttt
    38821 gaaggactag tatctggaga taagtatgga tctgctgtag ggatcgctgc attgatcctt
    38881 gttgttggag gtgcttttgg tattattatg cgtaccggag cgatcgacgc aggtatctat
    38941 gcgtttatca gtaaaactaa gggactggag cgtttagctt taccgctatt attctttgct
    39001 ttttcattcg gtggcgctac attcgggatg gcagaagagg taattccatt ctcaatggtg
    39061 atggttccat ttgtcattgc gttaggatat gactcgatcg tagcagtaac cgtgacttat
    39121 gtcgcatctc aagtcggaaa tgcgacctct tggatgagcc cgtttagcgt agcggtggct
    39181 caagggattg ctggaatccc cgtgttgtct ggtgcgacat tccgattgat tatgtgggta
    39241 gttgttactg cattagccgc tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa
    39301 ccagaaagtt cgttgactta caaatcagat gattatttcc gttctcatat caaaaagaca
    39361 tctgatgaaa ataaaaaatt catgcttggg cataaattga tcttattaga gatgttagtt
    39421 gtccttgtct ggatcgtttg gggcgtgact caaaaaggct attacattcc agaaatcgct
    39481 tctcaattct ttgtgatggg attagttgct ggaatcattg ctgttctttt caaattagat
    39541 ggaatgaaag tcaatgacat cgcttcttct ttccaatctg gtgcagctga tttagctggt
    39601 acagcaatcg tagtaggtat ggccaaaggg attcttcttg tcttgggagg atctgatgca
    39661 tctgttcctt cagctttgaa tacgattttg catgggatcg ggacggcgtt gaccggtgtt
    39721 ccagctgtca ttggtgcttg ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt
    39781 acatcaaatt caggacaagc agctttgact atgccaatca tggctccact ggcagattta
    39841 gtaggtgtat ccagacaagt agcagttctt gcttatcagc ttggttcagg ttttatggac
    39901 gcttttacac ccgtatcagc cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg
    39961 gcaaaatggg caagattcca aatcaagatg caaggattcc tgtttgtttt aggaactatt
    40021 ttcatcatga ttgccattgc aataggtctg cagtagtact ggagacctgt aaataaaaaa
    40081 gtaatccaaa cagtagataa ggactccaag aaccgtcatt ttacggtaaa atcaaaggag
    40141 taaagatcct atcgtttgga ttatttttta agatcctatc atcagttata cttaaagaaa
    40201 gaggaaaaat gtatgaaaat cattcgacaa attgaagtgt ttgcaccaga ttacttagga
    40261 aaaatggatg tactgattgc cggtgggaag attttggctg tagaagaaca gctagaaggc
    40321 ggctacgaag gagtggaagt agaagaactt tcaggtgagg gaaaagtact aaccccgggt
    40381 ttcattgact gtcatttcca tattttaggt ggaggaggag aaggcggtta tcaaaatcga
    40441 acgccagaag tcacattgag ccaattgact acagctggtg taacgacagt tgtcggttgt
    40501 ttgggaacag acggagaagg tagagatatg actgcgttga tcagcaaagc gaaaggtctg
    40561 gaagcagaag gaatctccac ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca
    40621 gtaacagatt ctatcatcaa agactttttg acaattgata aaattatcgg aatcggagaa
    40681 atcgcagtat ccgatcaccg ttcttctcag ccaagttttg aagagttcgc tcgtgcagta
    40741 gcagatgctc gtgtaggagg catgttatcc ggaaaagctg ggatcatcaa tgtccactta
    40801 ggcggaggaa aaagaaagct agagctattg acacgaatcg tagaagaaac agaaattccg
    40861 atcacacaat ttttgcctac tcatgcaaat cgcacaccag aattatttga agcttgcgtg
    40921 gcatttgcca aacgtggtgg cacgattgac ttcactgcta gcgaagatcc tgatttttgg
    40981 gagaagacag atggtgaagt tcgcttcagc aaagcactga aacgattgat cgaggaagat
    41041 gtttctttag ataatttcac gatgacctca gatggacaag gaagcttgcc ttattttgat
    41101 gaaaacaatc atttcctagg gttaggagta ggaagtgcca aggcattgct agtagggatc
    41161 aaagaagctg tgcaaaaaga aagtatccct ttggaaatcg ccttacgagc tataaccagt
    41221 aatccagcac gtatattgaa attggataaa aaaggaaaaa tcgaaattgg tgctgatgca
    41281 gatctgtgta tcttggataa agaaacgctt gatattgata cagttatcgc taaaggagaa
    41341 ataatggtcc aagaaaaaga agtcaaagta tgggggactt tcgaaaaaag tttttaatca
    41401 acagctctaa ctatcttttt tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat
    41461 gttaccctga aggtagatca ataaacaaac taagaggagg aagcacaatg gcagatttaa
    41521 aaggacgttc taaagatatg aaagataaag tagtcggtga aggaaaagaa gcatacggca
    41581 aagcaacaga cgacaagagc aaagaaacag aagggaaagc acaatcacta ggtgctgaca
    41641 tcaaagaaaa agctcgaaat ttaggcgacg aagtcaaaga caagttttct cgaaaagacg
    41701 aaaaaaaata aaaaccactt attactaatt gaatcaagca aataaaaaat ccgaaccata
    41761 tctgcgagac agatatggtt cggatttttt atttgatcat tatcctgttt tttaaacttc
    41821 gattttttga aattttttat tttttagttc aaagacttca tcacagcttt ttgctacttc
    41881 aatctcatga gtcacgatga taatacagcg attttgttca tgggcgattt cttggaaaag
    41941 tttgacgata tcttgtgaag ttgtttcatc tagatttcca gtaggctcat ccgctacgat
    42001 tagttgatgt tcacagcaga tagcacgaac gatagcgact cgctgctgct gacctcccga
    42061 aagtttggtc acctgttttt ttgctaatat ttcatcaatt cctacacgag ccagtcgatc
    42121 taaagcaact tgtcgataat tctcttgctt ggtcttagtg atttccattg ctgctatgac
    42181 gttgtctaga gccgataaat aaggaagcaa gttgtaagct tggaaaataa tcgagacatc
    42241 atttttacga taacttctta acccgatttt gttcagtgaa cgatcttgat agtatacctt
    42301 tccttctttt ggtgcatcta gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc
    42361 acttgctcct aaaatagcgt acatttttcc agcttcgaaa ttcagttcta catctttata
    42421 aagcgcatct tgcggatctt gataccaaaa tcccaattgt tctgtccgta acatcaaatt
    42481 cctcctttag tttaatagga tttttcttgg gtttaatctt aatatcccta tagaagctaa
    42541 aaagacagaa aacagactga ttccaaatgc tactccggct aatagtccca attgtgcagg
    42601 tttgacattg atttccaatt cgctgacttg atcagaaaca gcaaatggat tattcgtggt
    42661 tccttgttga ccatcgcgtt gaccgccttg ttcttgtctg ttacctggca tttgtccacc
    42721 gccaggagct tgctggtttt gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc
    42781 cacggcattc gcgacgacgt ttccgctaag cgaagcaact cctaatgcaa gaatcataca
    42841 aatagccact tcggtaaaca attggagaat gattttactt ctggattctc ctaaagatag
    42901 caagacgcct aactcatgcc gacgctcccg aatggtaatc ataatgatca gagtaagtat
    42961 gataataccg gctacagcca ctagtaaaac aacattttta gaaaaacttg ccacgttgtt
    43021 taaaggttcc aacattgact gatacatgga atcatttgat tgtaagctaa atgtatctgt
    43081 gtcgatcagc ttctcagctt ctttgagaaa ctcatccatt ttatctggat cagatagagt
    43141 gtaaatagca gaatctatcg tgttatcgtc agatgtccca tttagttcat ttgcaaaagt
    43201 gtaagaagtg taaagtgtat tcgacggatt catgaagttg aatttcatac ccatactgcc
    43261 gcttgtttct ctggattcat aaattccttt gatcgtcatt tcataagtgg tatcttcatc
    43321 ttcagtactc gtgattgtga aagtatctcc gactgccagg tcatttgttt ctgctagagt
    43381 agaatcaatg actacattgt ttgtgccttt gtcgctgtca tctattcctt caccatccgt
    43441 gatttttgct gttccatcag aaaaattact attttgtgca gttgctgaca caccactgat
    43501 ttgaaagtct gcttgagtca tttgcggatt cccgcccatt cctccagcag gttcaccgcc
    43561 tgcatcagta gtttctgaac tgtcttcact tgaatcagaa ctagtgatgg cagttattcc
    43621 atcttttgcc atagctgagg cggaactttc aaaggaataa ctctttacat tatctaattc
    43681 agctattttt tccgcgtccg ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg
    43741 acgttcttca gtggtagaac cgcttgaaga agtttgttcc tgttttttaa atgaagcttc
    43801 tcgattggca cttaaagtca ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc
    43861 ttctgcagcg cttcgaatcg tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac
    43921 ggcaaacaat aacaatgaac gtccccattt tacttttgta cttttccatg cacgtttcat
    43981 aaaattcata ttttgcatcc ctcctagtag atgcatttga taatagacaa cctatcttaa
    44041 agctagctta aatcttatgc tgtttgcaca ccatcaacat gaggttctgt atatataaaa
    44101 agtcactata gttattgcaa aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg
    44161 aagaagaaat gaaagaggaa tagcaaaaga aatcctagat gctacagagg gatttttacg
    44221 tgcatagttg ttctaacaat tgctgatact agcgatttag tttatttgtt gtataataat
    44281 ggggaacagc tgaatactta tataagtatt ctggctatag cgtttcgcaa aagatccatt
    44341 ttgttaatga agtatatcca aaataaaatg cccatccaac tgtcagaacg cggatgtgct
    44401 ggtaattttc ctgctgattc tccataagtt gagaaatagt gattttgcat atgcttcatt
    44461 ggaatggctc atatatttag ataaagaaga aaatcgacat ctttaaactt ggatgtcata
    44521 agaagaggtg aagtttgtga gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa
    44581 tggaaaaaac atgtatgtcg cagaagtggg cgaggatatt tttattttag attgtgggct
    44641 aaaatatcct gaaaatgagt tgttggggat cgacgtcgta atccctgatt ttacgtattt
    44701 agaagaaaac attgatcgtg tagctggtgt ctttttaaca catggacatg cagatgcgat
    44761 cggggcactt ccttacttgt tgtcaaaaat ttctgtacca gtattcggga cagagttgac
    44821 gatcgagtta gcaaaattga atgtcagtcg taatgaagca gccaaaggat ttaaggattt
    44881 ccatgtggta gatgagcata cagaaataga ctttggtgaa acagtagtga gctttttccg
    44941 taccactcat acgataccag attcagtggg gatcaatctg aaaacaaaag aaggtagtat
    45001 cgtttatacg ggagatttta aatttgacca aacggctatc ccaatgtatc aaacagatta
    45061 tgcacgattg gcggaaatcg gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa
    45121 tgcagaaaat cctcagcctg tagcttctga agcccaaata gcagatgaag tatttgatac
    45181 gatcaaatat tgggaagggc gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt
    45241 gcaacaagta ttggatgcag cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga
    45301 tttcgaacga attattcgca cagcgatgcg tttagaaaaa cttcaattac caagtgaaga
    45361 tctgcttgtt acgttgaaag acatgaaaaa ctatgatcca gaagaactga tcattttaga
    45421 aactggccga atgggtgaac ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg
    45481 taatgtcaag attgaagaag gagatttggt ttatatcact actacgccaa gcatcgcgat
    45541 ggaaaccatc gtggcgaaaa cggaagatat catttatcga gctggtggaa cagtgaagtt
    45601 gatctctgag aatatgcgcg tttctggcca tgccaatcct aatgacttgc aattaatgat
    45661 caatttgatg aaaccaacat attttgtgcc agttcaagga gagtatcgtg aattagctgc
    45721 tcatgcagat ttggctcatg cagtagggat gccatacaaa aatatctata ttactggacg
    45781 cggagacgtg ctggaatata agaacaagcg tatgagtgtt gcaggaacta caagtgcaga
    45841 aaatgtaatg attgatggat taggcatagg tgatatcgga aacattgttt tacgtgatcg
    45901 aaaaatcttg tcagaagacg ggattttcgt agcggttgtc actatcagtc ggcgagaaaa
    45961 gaaaatcatt tcaaaacctc aaattacttc acggggattc gtttacgtaa aagctagccg
    46021 tgatctgatg aaagaaagca gcaatatgat tgaaacaatc gttgagaaac atttagaaag
    46081 caatgatttt gagtggagca aattaaagca agacatccga gatcaattag gccgttactt
    46141 atttgaacaa acaaaacgcc gcccagtgat cttgccagtc attatggaag ctactcaacg
    46201 tcgaaataaa aaataaacaa aaagagtaag atgaacctct ctatagagtg ctcgtcttgc
    46261 tctttttttg cgaaaaacaa ataatttttg aatacagcat ccctcgcttt ataataaacg
    46321 atagaataaa gggagtgaat caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga
    46381 acaaaagatc agctatcaac cacttgattt tagtcatgtg gtagaagaat ctttcacaga
    46441 agaactacaa aaaagaggga ttgatccagc tttgatatgt aaaactctcg tagtgaaagg
    46501 aaacaaaact ggggtgatca tcgcattgat tcctttgaac gatcacttag attacaaaaa
    46561 aacgagacaa gtgacgaaag atcgtaaagt tggttttcct ggaatggatt ttgtattaat
    46621 gcatacagga tatccccacg gagcaaacac ccctattgga ataaaactcg cacatcctga
    46681 ttattgtttt ttagcagaca gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg
    46741 tgaaatcggg agaagccttc ggataaaagt agccgattta gaaaagatcg tccatcctat
    46801 ttacgctgat ttaattagtt aagggacgag aagagaagct aagcagagca gagacctcaa
    46861 acttgagatt atatctgctt ggtacttggt gcatcctgta ttatctgtta caattgcttt
    46921 gtacatactg tactatcaat ccaatgaaaa tatatttagc agctatattt tttcattgat
    46981 gtgcaagtga tgaaatcgtt tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat
    47041 atgaaaatta cgtatcatgg acattcctgt ttaacaattg aaatggaaaa tggccaacgc
    47101 gtgatgattg acccgtttat tacaggaaat ccattgacag acttaactgc agaagaagta
    47161 aagacagatt ggttgctgat tactcatggt catagtgacc acataggaga tatgattcct
    47221 attgcaaaag aaaatcaagc cacaattatc agtattgttg agattgctga ttatgcgaac
    47281 agcagaggag tagattcatt cggtatgaat atcggtggaa aacatgcgtt tccattcggc
    47341 gtggtaaaat tcgtacatgc acagcatagt tcaagctatg aagtagacgg tatcgttcag
    47401 tatatgggcg agcctagcgg aattattatt caagcagaag gaaaaacgat ttatcatgca
    47461 ggggatactg cttatttcag tgatctagga ttgcttgcag aagaattcga tattgatgtc
    47521 gctttcttgc cgattggcga taactatacg atggggccag aagatgcagc aaaggctgcg
    47581 caaacaattc gtgccaagaa agtagttcca atccattaca atactttccc tgtcattgaa
    47641 caagatcccg aatcttttgt agagttactg gaagatagtg aaggaaaaat aatgagcgtc
    47701 tcggaaacgc tggaagtgta ggcagaaaat ggcgacaaaa catgatttga ttttagaata
    47761 tattgaaagt ttacctgttg gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa
    47821 tgtcagtgaa ggaacagcgt accgtgcaat caaagatgca gaaaatgtcg gactagtttc
    47881 aacaattcaa cgagtaggga ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg
    47941 tttgacattt ggcgaggtcg tcaggatcat tgaaggggac gttttaggcg gggcggcagg
    48001 attagacaag gtcttgaata aattcgtcat cggcgcgatg actaaacatg ccatggaacg
    48061 ctatattacg ccaggatcac taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc
    48121 attggaagat ggcgccgcag tattgatcac aggaggattc aatacgactg ctgagattgc
    48181 aaagctagct gatgatcttg aaatgccgat cttacgcacc tcttatgata catttacagt
    48241 cgcaaccatg atcaaccgag cgttaagcga ccaattgatc aaaaaagata tcatgcttgt
    48301 cagtgatatc tatatgccag ctgaaaagac tcattatttg cagcagacag atacgatttc
    48361 tgattatcag cgtttgtcag aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca
    48421 tcatcgtttg atggggattg ttacggcaaa agatgtttta gggaaatcgc caaaccagat
    48481 catcgatcgg gtgatgacga aagaaccaat cagtgtaaag aaaacaatga gtattgcctc
    48541 tgttagccat caaatgatct gggatgggct agaagtcatg ccggtcgttt ccgatgactt
    48601 gacattggaa ggcttgatta cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg
    48661 acaaccgcag attgcagata cgatttcgga tcagatatct ggaaatatcc atacgattga
    48721 tactgaccgt gaaggaaatc gtttggaaaa ccctaagttc aaattcgtag tagcaccaca
    48781 aatggtcaat ggcgttggaa ccatttcttt tggcgtactt agcgaaatca tttctgatgt
    48841 cgcacaaaaa acaatggtga tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct
    48901 acattatttg cggctgatcc aattggaaag cgaattagac attcgtccac gtgtattgga
    48961 aatcggcaga cgttccgcta aactggatat cgaagtgttt attgaaaaca ccatcgtttc
    49021 caaagcaatc gttgtttgtc aagtaatgga acgttcttag aaagcaggag tgaaatgaca
    49081 gtacagcaag aaatagtaga tacaataaaa aaatacgata aaatcattat ccacaggcat
    49141 ttgcgccctg atcctgatgc attaggatca caagtaggat tagccgaact attacgggaa
    49201 agtttcccag aaaaagaaat acgtcaagta ggtgaaacga ttgaagggct tcgcttttta
    49261 gcagaaatgc aagaagttga agatgtcttc taccaaggag cgctagtcat cgtgactgat
    49321 acagctaatt cacccagaat cagtgatcaa agatatcagt tggcagaaaa actgatcaaa
    49381 atcgaccatc atcccaatga tgacccttat ggagatcttg tgtgggtaaa tacaaaagca
    49441 agtagctgta gtgagatgat tgcagaattt gctttgatgt ttcctgaatc tttaaagatg
    49501 aacgttaagg ctgcacggtt actttatgca ggaatcgtcg gagatacagg gcgctttttg
    49561 tatccctcca cgacgtcccg tacattagag attgcttcga tattgagaaa ttatccattt
    49621 gatgcaagtg ctttgaacag agagattgaa caaatgccga tgaaagtcgc taaactttcc
    49681 ggttatcttt atcaaaatat ccaggtggat gaaaatggtg caggaagaat aatccttccg
    49741 caagaaattc ttgataaata tggaatcgat gattcagaga cagcttcgat cgtttcactt
    49801 cctggagtga ttgacgaagt gctggcttgg gggatttttg ttcagcagcc ttcagggtat
    49861 taccgcgtac gtttacgttc taaggggccg atcatcaatg aattggctaa acgccatcat
    49921 ggaggaggcc atccattagc aagtggtgcg aatgcaaaag atttagcaga agtagaggaa
    49981 atctataaag agatccaaga tatatgccaa tcgtataaga gcagaaaaac agaatgatac
    50041 ttcgttcgaa aacgtgcctt ttgtagtaaa aatcacaaat gtatcttttc aagatcttct
    50101 ttttccgtta caatagtgct cactaaagat tttaaaggag aaagaagatg acattaccaa
    50161 attgcccaga atgtggctca gagtacacat atgaagatcg aggcttgttg atttgcccag
    50221 aatgcgggaa cgaatggaac aaagaagaac aagaaactgt catagaagac ggattagtgg
    50281 tattagatgc aaatggaaac cgtctgttag agggcgatag cgtgacagtg attaaagact
    50341 tgaaagtaaa aggtgcgtca ggtgccatta aacaaggaac aaaagtcaag aacatccgtt
    50401 tagtagaagg cgatcataat atcgattgca aggtagaagg atttggaccg atgaaactga
    50461 aatcggaatt tgtgaaaaag aattaaagaa tattcaaggt cttttcacat tcttttccta
    50521 ctactaatga taaaataaag taaacgaaaa ggagtgaaac gaaaatgtat ggttatggaa
    50581 tgtatggttt tttagatccg acatttcttt tggtcattct tggattagtt atttccggaa
    50641 ttgcgtcagc gtacgtcaat agtactttcc gaaaatacga tcaagtgaga agcaaaaaca
    50701 atgtgactgg tacacaagca gcacaatata tattacaaag tcaacagata aataatgtcg
    50761 gtgttcaaca aattgccgga gatctcacag ataactacaa ttcaggtaat aagatgttga
    50821 gtttgtctca agcaactgca cagtctactt cagttgctgc gataggtgta gcagctcatg
    50881 aatgcggaca tgcggtacaa gatgcttcgg gttatgtccc attgaagatt cgtgcagcac
    50941 ttgttccagc agctaacttt ggttcgatga tttcgttccc cttgattatg gtaggtgtat
    51001 tgcttagctg gaactctacg ttaatcaata tcgggatttt cgctttttct ttagcattac
    51061 ttttccaatt ggtgacatta ccagtcgaat ttaacgcgtc tcggcgagca atccaaattt
    51121 tgagtgaagg cggactactg acggaagaag aagtaccgat ggcaagacac gtattatttg
    51181 cagcagcact gacttatgtt gcagcagcgc tttcgacatt cctgcagctg ttgcgcttga
    51241 tcttattgtt cggcggcaac cgaagagatt aaaataaaga agttgtgaaa tcagcgcttt
    51301 gatctgagct aataccgaga gttagctgat tgaataccat ttatttggaa
//
LOCUS       DANKBF010000015        48714 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000015 DANKBF010000000
VERSION     DANKBF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            33..656
                     /locus_tag="PWY89_000895"
     CDS             33..656
                     /locus_tag="PWY89_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065951.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="PWY89_000896"
     CDS             1106..2155
                     /locus_tag="PWY89_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2065952.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="PWY89_000897"
     CDS             2152..3033
                     /locus_tag="PWY89_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065953.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="PWY89_000898"
     CDS             3036..3806
                     /locus_tag="PWY89_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2065954.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="PWY89_000899"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="PWY89_000899"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2065955.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="PWY89_000900"
     CDS             5117..6280
                     /locus_tag="PWY89_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2065956.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="PWY89_000901"
     CDS             6397..6807
                     /locus_tag="PWY89_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2065957.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="PWY89_000902"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="PWY89_000902"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2065958.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="PWY89_000903"
     CDS             9380..9664
                     /locus_tag="PWY89_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065959.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="PWY89_000904"
     CDS             9824..10060
                     /locus_tag="PWY89_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2065960.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="PWY89_000905"
     CDS             10057..10455
                     /locus_tag="PWY89_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2065961.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11122..12066)
                     /locus_tag="PWY89_000906"
     CDS             complement(11122..12066)
                     /locus_tag="PWY89_000906"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2065962.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12362..13306)
                     /locus_tag="PWY89_000907"
     CDS             complement(12362..13306)
                     /locus_tag="PWY89_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065963.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13526..14740
                     /locus_tag="PWY89_000908"
     CDS             13526..14740
                     /locus_tag="PWY89_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2065964.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14762..16777
                     /locus_tag="PWY89_000909"
     CDS             14762..16777
                     /locus_tag="PWY89_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2065965.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16860..18233)
                     /locus_tag="PWY89_000910"
     CDS             complement(16860..18233)
                     /locus_tag="PWY89_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2065966.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18259..18543)
                     /locus_tag="PWY89_000911"
     CDS             complement(18259..18543)
                     /locus_tag="PWY89_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2065967.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18545..20560)
                     /locus_tag="PWY89_000912"
     CDS             complement(18545..20560)
                     /locus_tag="PWY89_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2065968.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20739..21044)
                     /locus_tag="PWY89_000913"
     CDS             complement(20739..21044)
                     /locus_tag="PWY89_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL2065969.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21133..22163)
                     /locus_tag="PWY89_000914"
                     /pseudo
     CDS             complement(21133..22163)
                     /locus_tag="PWY89_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            22370..23245
                     /locus_tag="PWY89_000915"
     CDS             22370..23245
                     /locus_tag="PWY89_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2065970.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23397..24239)
                     /locus_tag="PWY89_000916"
     CDS             complement(23397..24239)
                     /locus_tag="PWY89_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2065971.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24331..26208)
                     /locus_tag="PWY89_000917"
     CDS             complement(24331..26208)
                     /locus_tag="PWY89_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2065972.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26205..27626)
                     /locus_tag="PWY89_000918"
     CDS             complement(26205..27626)
                     /locus_tag="PWY89_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2065973.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27629..31018)
                     /locus_tag="PWY89_000919"
     CDS             complement(27629..31018)
                     /locus_tag="PWY89_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2065974.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31275..32666
                     /locus_tag="PWY89_000920"
     CDS             31275..32666
                     /locus_tag="PWY89_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2065975.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32739..33386)
                     /locus_tag="PWY89_000921"
     CDS             complement(32739..33386)
                     /locus_tag="PWY89_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2065976.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33394..34080)
                     /locus_tag="PWY89_000922"
     CDS             complement(33394..34080)
                     /locus_tag="PWY89_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065977.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34086..35159)
                     /locus_tag="PWY89_000923"
     CDS             complement(34086..35159)
                     /locus_tag="PWY89_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2065978.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35381..36055
                     /locus_tag="PWY89_000924"
     CDS             35381..36055
                     /locus_tag="PWY89_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2065979.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36060..37400
                     /locus_tag="PWY89_000925"
     CDS             36060..37400
                     /locus_tag="PWY89_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2065980.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37549..38535)
                     /locus_tag="PWY89_000926"
     CDS             complement(37549..38535)
                     /locus_tag="PWY89_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL2065981.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39259..40290
                     /locus_tag="PWY89_000927"
     CDS             39259..40290
                     /locus_tag="PWY89_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2065982.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40399..41931
                     /locus_tag="PWY89_000928"
     CDS             40399..41931
                     /locus_tag="PWY89_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2065983.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42070..42882
                     /locus_tag="PWY89_000929"
     CDS             42070..42882
                     /locus_tag="PWY89_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL2065984.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42872..43357
                     /locus_tag="PWY89_000930"
     CDS             42872..43357
                     /locus_tag="PWY89_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2065985.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43380..43925
                     /locus_tag="PWY89_000931"
     CDS             43380..43925
                     /locus_tag="PWY89_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL2065986.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(43998..45641)
                     /locus_tag="PWY89_000932"
     CDS             complement(43998..45641)
                     /locus_tag="PWY89_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2065987.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45770..47998)
                     /locus_tag="PWY89_000933"
     CDS             complement(45770..47998)
                     /locus_tag="PWY89_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2065988.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(48031..48510)
                     /locus_tag="PWY89_000934"
     CDS             complement(48031..48510)
                     /locus_tag="PWY89_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2065989.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKRSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        14830 a         8971 c         9168 g        15745 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt
    11101 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
    11161 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
    11221 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
    11281 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
    11341 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
    11401 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
    11461 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
    11521 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
    11581 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
    11641 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
    11701 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
    11761 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
    11821 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
    11881 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
    11941 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
    12001 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
    12061 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
    12121 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
    12181 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
    12241 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
    12301 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
    12361 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
    12421 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
    12481 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
    12541 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
    12601 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
    12661 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
    12721 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
    12781 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
    12841 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
    12901 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
    12961 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
    13021 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
    13081 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
    13141 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
    13201 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
    13261 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa atgtctaatt
    13321 cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac
    13381 gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg
    13441 acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat
    13501 aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct
    13561 gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat
    13621 gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc
    13681 ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa
    13741 acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc
    13801 acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat
    13861 caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat
    13921 gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg
    13981 acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat
    14041 tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg
    14101 cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt
    14161 tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac
    14221 gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc
    14281 aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct
    14341 tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct
    14401 gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta
    14461 cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat
    14521 cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc
    14581 ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt
    14641 gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag
    14701 agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa
    14761 aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg
    14821 ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg
    14881 gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc
    14941 ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca
    15001 tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg
    15061 acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg
    15121 ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga
    15181 tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga
    15241 aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga
    15301 actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt
    15361 agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa
    15421 agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca
    15481 acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc
    15541 ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt
    15601 gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc
    15661 attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc
    15721 attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt
    15781 gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat
    15841 cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg
    15901 gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga
    15961 aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg
    16021 cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca
    16081 agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg
    16141 cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt
    16201 cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt
    16261 attgaatgac actatcacac agattactac tccatatttc aaattctatg aacaagatgc
    16321 tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg
    16381 gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa
    16441 tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc
    16501 aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta
    16561 tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc
    16621 tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga
    16681 tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc
    16741 acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata
    16801 tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct
    16861 taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat
    16921 acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct
    16981 agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg
    17041 attttaagat ctcctaatac tggaaggata aataatggca gccaactaat caataagcta
    17101 ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca
    17161 gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag
    17221 cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat
    17281 acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt
    17341 acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc
    17401 ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt
    17461 acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca
    17521 agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag
    17581 ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt
    17641 ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg
    17701 tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt
    17761 ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact
    17821 aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat
    17881 ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa
    17941 gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact
    18001 actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg
    18061 attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg
    18121 atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg
    18181 aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt
    18241 tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt cttttacttc
    18301 ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt
    18361 atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac
    18421 atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt
    18481 gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt
    18541 catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta
    18601 ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa
    18661 gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat
    18721 tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc
    18781 catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat
    18841 actgctccac gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata
    18901 atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt
    18961 ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta
    19021 aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt
    19081 ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc
    19141 ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca
    19201 tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag
    19261 aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct
    19321 ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc
    19381 taatgccact tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg
    19441 cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc
    19501 tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag
    19561 catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac
    19621 gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa
    19681 attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa
    19741 aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa
    19801 atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa
    19861 tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt
    19921 ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg
    19981 cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat
    20041 aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca
    20101 tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc
    20161 taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta
    20221 tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa
    20281 aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt
    20341 gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg
    20401 ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt
    20461 gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt
    20521 ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact
    20581 tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga
    20641 ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga
    20701 ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt
    20761 tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt
    20821 tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa
    20881 gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct
    20941 tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt aggggccatt
    21001 gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt tcctcctagt
    21061 agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac aaacccgatt
    21121 cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc cttcttccaa
    21181 aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc cattaactaa
    21241 tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa ttactttttc
    21301 ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt cgggcaaatc
    21361 gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt cttgtcgatc
    21421 ttttgtttgt ttcacgaaca tccctttttt ccgtaaacga cgaaacttgg acgttctttg
    21481 atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg
    21541 aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct
    21601 ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag
    21661 ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa
    21721 tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc
    21781 aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt
    21841 gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt
    21901 acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta
    21961 gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat
    22021 tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg
    22081 attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg
    22141 ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc
    22201 ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac
    22261 ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata
    22321 gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga
    22381 agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat
    22441 ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta
    22501 tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga
    22561 attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat
    22621 tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc
    22681 ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga
    22741 tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc
    22801 tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat
    22861 ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc
    22921 catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa
    22981 gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca
    23041 ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt
    23101 ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca
    23161 aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct
    23221 tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg
    23281 tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct
    23341 cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa
    23401 cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt
    23461 atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact
    23521 tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga
    23581 ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt
    23641 ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt
    23701 gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg
    23761 aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc
    23821 cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc
    23881 gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta
    23941 tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct
    24001 gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg
    24061 attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc
    24121 tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt
    24181 cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt
    24241 tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg
    24301 ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa
    24361 gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt
    24421 accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa
    24481 ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg
    24541 aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt
    24601 cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac
    24661 ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg
    24721 atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac
    24781 tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt
    24841 atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt
    24901 caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt
    24961 cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc
    25021 gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa
    25081 tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc
    25141 ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc
    25201 gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt
    25261 tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt
    25321 ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc
    25381 aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt
    25441 gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt
    25501 ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc
    25561 ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt
    25621 tgtaactttc aacgttccat cattacagac tgtattttta gggtagagat ggatagtatc
    25681 tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg
    25741 gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt
    25801 gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc
    25861 atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc
    25921 tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac
    25981 tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct
    26041 gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc
    26101 tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat
    26161 aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct
    26221 gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct
    26281 aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta
    26341 ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta
    26401 aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga
    26461 taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga
    26521 agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt
    26581 cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc
    26641 tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt actgacacca
    26701 aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt
    26761 agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc
    26821 gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt
    26881 tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta
    26941 tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa
    27001 taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa
    27061 ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt
    27121 tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg
    27181 acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta
    27241 gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct
    27301 gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc
    27361 gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg
    27421 acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga
    27481 tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct
    27541 gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac
    27601 atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa
    27661 gataccaaat agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa
    27721 ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt
    27781 tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc
    27841 catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa
    27901 aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt
    27961 cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc
    28021 cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc
    28081 gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa
    28141 tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg
    28201 caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa
    28261 gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt
    28321 tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat
    28381 acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat
    28441 catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga
    28501 tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg
    28561 tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat
    28621 gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat
    28681 ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag
    28741 tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt
    28801 tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct
    28861 cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat
    28921 ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat
    28981 ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt
    29041 tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg
    29101 gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt
    29161 tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt
    29221 aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc
    29281 cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa
    29341 gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta
    29401 cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat
    29461 cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag
    29521 actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac
    29581 catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg
    29641 tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta
    29701 gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc
    29761 ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca
    29821 aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg
    29881 ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt
    29941 tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg
    30001 agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga
    30061 caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg
    30121 accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag
    30181 cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg
    30241 catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag
    30301 gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat
    30361 gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat
    30421 catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg
    30481 ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt
    30541 ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa
    30601 taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg
    30661 gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa
    30721 ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc acatcactta
    30781 agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat
    30841 ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg
    30901 tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg
    30961 ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt
    31021 ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac
    31081 aggcgttaca taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg
    31141 gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat
    31201 gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaat
    31261 gcaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga
    31321 ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg
    31381 aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt
    31441 atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa
    31501 gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt
    31561 attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca
    31621 atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta
    31681 gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg
    31741 gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga
    31801 ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta
    31861 tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg
    31921 gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc
    31981 tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga
    32041 ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata
    32101 tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc
    32161 aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga
    32221 cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg
    32281 gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt
    32341 tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg
    32401 ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat
    32461 atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag
    32521 atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt
    32581 tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag
    32641 aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg
    32701 ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc ttcagcaatc
    32761 caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt atttgcaatg
    32821 agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact aactgcttct
    32881 tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt ttcgttcatt
    32941 ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg ctgggttaac
    33001 aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca ctctaaatat
    33061 ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa gatatcttga
    33121 tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa atcttgaaaa
    33181 tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga gactttgact
    33241 tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct ctgtttcttt
    33301 gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt atctctgatt
    33361 atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct cttcttctgt
    33421 caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac ggacatcatg
    33481 agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta atttgacgac
    33541 ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga tcaagcttgg
    33601 ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag aaaggtcttt
    33661 aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat cgatttttgt
    33721 tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct ttaccgttaa
    33781 aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc gtaaatctgc
    33841 tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt ctccggaagt
    33901 cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac cagaaggacc
    33961 aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca aagctgagac
    34021 ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata tttttttcat
    34081 ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg tatcgaaaac
    34141 aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca ttgtgaccaa
    34201 aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc aaacaaaaga
    34261 gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga tttagcgatg
    34321 aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc
    34381 atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa gtatatcatc
    34441 gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa tgtcccaata
    34501 gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga atcctttatt
    34561 acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa
    34621 gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata agtttcagga
    34681 aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt atctccaatt
    34741 ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt tttaaattca
    34801 tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa aactgtaaca
    34861 ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc tttctctcct
    34921 ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca
    34981 tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt aaactcttct
    35041 gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa gaacataata
    35101 ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc tagaaacatt
    35161 tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga atagtgtaac
    35221 tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt gtctaagaca
    35281 tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta tttatgcttt
    35341 ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac tattagtagt
    35401 agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa tcggcgagat
    35461 aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag tttatgatct
    35521 tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag caaaaatacg
    35581 gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt tggaagataa
    35641 gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct atcgagaaga
    35701 actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg atgaaaatgt
    35761 tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca
    35821 agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac aaaataaggg
    35881 aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt ctgatacgac
    35941 gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa gtactggatt
    36001 ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa
    36061 tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat
    36121 ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac caatctgcat
    36181 acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca gtacaattag
    36241 agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca
    36301 gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac actcaagtga
    36361 tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg cgtttcaacc
    36421 aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat attgaattaa
    36481 atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa gcggagacga
    36541 atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc aagaattcat
    36601 tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt ctctctattg
    36661 gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct tggagaagac
    36721 aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg attattcaaa
    36781 atagtttgga acatcttttt accaagccta atcatactat tattgaagaa tccgaaagca
    36841 tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat ttgctgacaa
    36901 tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat acacaagaat
    36961 atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga aaagagttta
    37021 ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc catcaagttt
    37081 tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag atcattttgg
    37141 attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc ccttctatca
    37201 gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa tctcgggcga
    37261 aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc gaacagcatc
    37321 atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa gtccaattgc
    37381 cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca
    37441 cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg aaataagccg
    37501 aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt acgatttttt
    37561 attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt ctcggctaac
    37621 atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa cttcttcacg
    37681 aataactgta tgtttgctta catctacttg ctcttctttg attgggatag taatcgtgtc
    37741 atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt tgcgttcgat
    37801 gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga ccacatgttt
    37861 gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt tttcttcttt
    37921 tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt
    37981 acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg tcatattcaa
    38041 tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat ctgttccagt
    38101 gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt gtgtatccat
    38161 tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa attttccatc
    38221 agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg tctcagaatc
    38281 ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt cttcttcacc
    38341 atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa atcctagtct
    38401 agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc cttcgttacg
    38461 caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga tgaccgttaa
    38521 atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt atattgttac
    38581 ttataaattt attctatagg agacgattgt tttctacaaa tatttagatt ggaatttgat
    38641 taaaacaatt taattgaaat ttgaaaacat ttatgttaat gaaagtcata ttctaataaa
    38701 atgtcatttg tttctatatg aatttttatg aaaaatttca tataatttcc actcgttgtg
    38761 atggtttaat ttatccaatc catcaataag caaacaactt atataaaaaa ggattaattc
    38821 tcaatataat tgctcctacc atagaataaa tcttcatgta aagtaatgaa gtaccaacta
    38881 tttctaatac ttctttaaga attatcccaa caatatcgtt agacaaacca ttattgaata
    38941 aatggagagc caatcgattc tgaaaaaatg ttttctaatt tttcggaaat aaaagaaaga
    39001 gcctcctgcc attagaacga accaaatcca tttcattgct catgaagtaa ccacatagct
    39061 aaaaagagga taaatcaatt aaaagatcaa aagaatgact gcactaataa aataactaaa
    39121 aatgtatctt cttacttcat atcttttagc tagtacttag ttaaaaattt gcttcctaag
    39181 atcaagacaa actgtctatc ttcatttgat gaaatgcggt aaaatataga aaatgaacac
    39241 tattggaggg aatttgtaat ggacagcaaa actagaaaac aactgcaaga aaagttaaag
    39301 caacgtacac acttgctccc ttccgtcttt caacagtttt tagaagaaaa tcgttcttcc
    39361 ctttcattag gcagtcgcta tgaatacgca aaagattttc atctgtttac tgattatttg
    39421 agaaaagtat tttctgaaga agtggatgat cagctgatta tccatttaga aaaacaagat
    39481 tatcagaatt tcttagctaa aatctatcga catactcgtt cgttccaaac tacagcgaac
    39541 caagaaacgg agcaattgtt tgaaaatagc tattacggga tttcaaggaa gcaatattca
    39601 ttaaatcatt ttttacgatt tttgtatcaa aaaggactga ttgaagaaga gatttctctt
    39661 ttgggtacaa gtttaccgga aacaaccaaa gcagctccgc gatatatcga tgcagaactt
    39721 ttcaaagatt ttgagcattt gtttatcccg attgaaggct ttcaaacaag cagagaagct
    39781 agctttgaag aaaagaatag accgaggaac cttgcaatta ctgccattct tctgtattgc
    39841 ggattaaaga tccatgaagt agtagaactt aaacgtaaag atgtatcttt gaccaagcag
    39901 attcttaatc tgaatagaaa agaaaacaga ctgaataaag taccaatacc aaaagaagcg
    39961 atgccatttc tggagaacta ttttaagtta acagcacaaa atacggacga aaattctttt
    40021 gtttttcgtt cctcacatga tcagcaaatt tctcctcgga ctattcgcca aattttgagc
    40081 aaaattacag tatataaaaa cgtatcgcca gaactatgca gaaaaacata tcgttattat
    40141 gcagaactat atttagatga tgctgacgcg gtaaattatt tatgtggtaa tcgatacctc
    40201 tcatcccatt cttttcaaga tatctgggaa aagatggttg atttttctta tcacgaatta
    40261 gcggcattcg ttcgggtttc ttccatataa aacaaatgta tgatcttttt ttcacaataa
    40321 actatgttat tatataaagg attatgttac gattatcttg aatttgtata ttacttaaca
    40381 aaagataatg gagtgtgaat gatggagacc ataacgtatt cccctttgaa cttatttacg
    40441 aattttcaag aagctgcagc taacttttcc gagaccccga ttatctttga tgagccactt
    40501 tcagctttcc cagaattagg acttgaaaca acttacaaaa aatgctgcga aagtgtgttg
    40561 aaacgggctt atcaattggc tcatcttgga gtgaaatccg gagacaaagt tattatttat
    40621 aagagttccg cttttgacac ataccttcta gctgtttctg tgtcttacct tggtgcagtg
    40681 cctgtcatga cttcttatca tctaccaaca acaacaatgg aagtatttat cgatcgttta
    40741 gaagaccctt tcattttatt tgatgatgag acaaaaaagc gagtgagaga aatttcaaat
    40801 ggaacaaaat caaaacagat tcctattctt tatttaatgg aacaacctgc tttgtctgtt
    40861 tctcaatcat ttttagacaa aaatgaaatt tcttatatga cccatacttc tggtacaact
    40921 ggtatcccta aacttatttg tcattctgct cattctatgg gatggcggac gaaatggcaa
    40981 aaaacagttt tcacaaaaat ttcggaaaag aaattaatcg cttttcacat ctctccagtg
    41041 cattccagat tcaatattgg tatctcttct ttgatgtcca tgggatttcc tatgatgcct
    41101 ttagcaaacg cacaaagttc aaaagttgtt catatgttgg aagctcaccg gccaattgca
    41161 ctagagaccc atccgaataa ctttgttcag tggcgtttta ctgctaagga gcatccagaa
    41221 gctttttcgg gtatccgata ctatcactcc acttttgatg ctataaacaa ccagacaatg
    41281 aagacatttt tgcgaacgtc gatggcacag gatgcggtat ttcttcaagt atatggccaa
    41341 agtgaatgcg gaccaatgat tcttaaggct catacactag agagtttgaa aacaagtgat
    41401 gcaagagata tgggcgttgg actggaagac atgacaagcg ctcggattac agattcagtt
    41461 gggaatgtcc tacctgaaaa cacagatggt catatacaat tactgtctaa aggacgggct
    41521 ttgacttatt acaaggaaga tgcacggttc caggaaaatg tttatggcga ttggtgggat
    41581 agcggagatt acggttttat ggataagcat ggccacctat ttttgaaaga tcggcaagtg
    41641 gatttgatcg aaacgatcaa tagtacatta gctattgaag actttttatt agattcgtta
    41701 gattttttgg cagaagtagt gattgtaagg gataaaaatc attctccaca accgattatt
    41761 gctttagctc ctgataaaga aatggattgg aatcgatggt gggagcaagt ccatgaactt
    41821 cctcgcttga acgtccctat catccgtgat tttgatgcaa tcccgcgaac agctacaatg
    41881 aaagtgcaac ggcttcaaat cgagaaagaa ttaaaatcac aaaagtccta actatttaag
    41941 gaaagaaaga tccattttgg tctttctttt ctttttgttt ttttaaattg gatgagataa
    42001 aaatatgcca attggctgtt tttctttttt gaaaatttcg ttatactctt attcatggag
    42061 gaaaatgtta tgaaaagaat cttgacaatt ggcggttctg atccatttgc tggtggagga
    42121 atccaaacag atttaaaaac ttttgagaat tttggtctat tcggactaag cgccttgact
    42181 tctatagggg cactagatat taatgagcga tttatgctgg aatctatatc tttggaattg
    42241 cttaaacgac agttagtttc gattgataaa atgacttcat tagatggtat caaaattggg
    42301 ttattgaatt caagtgaagc aatcatggtt gttcgagact tcataaaaac aaaacaaaat
    42361 atcccaatca ttttagaccc tgttttggca ttcaaagaaa ctgaagctgt gatcaatcaa
    42421 ttgtatattt cgcagctcat cgaagaactt tttccacttg ttacgatgat tacgcctaat
    42481 ttggcagaag ctgctttact ttctggtcaa gctcttccct cttcgttaac agaaatgatc
    42541 catctcgcta aagaactcac tgcttcagga acaaaaaaca ttgtgatcaa aggcggcaca
    42601 aggatggctg gagatgaagc tgtggatctt ctctattcaa ttgatcatta tgaggtattt
    42661 tctcataaaa aaatcgacac tacaacagta aacggggccg gttgtgcctt ttcttctgca
    42721 ataacagcga atcttgtgtt aggggcagat tttaccaaag cagtagcaga aagtaaaaaa
    42781 tttgttgccc attgtatttc taatggcgtg ctgatgaacg atcataccgg aagtgtctgg
    42841 tctggcggta ttcaaaaagg aggaatgaga aatgaaaact aagcaattaa cgatctatgc
    42901 tatgctgatt gcgttgacag ttgccctttc attaacaatt ttgatccctg taccggctac
    42961 gaatggattt gtgacattat gcgaggccgg gatctatacc gctgcttttc tttatggtcc
    43021 aacgggtgga ttagttgttg gtgcttccag tggtctattg atcgatttga tttcaggata
    43081 tccgcagtgg gcagtttttt cattagtgat ccacgggtta caaggatatg ttgctggttc
    43141 tttcagcaaa tcttctactc gtatgtgggg attagggctt ttattaggaa gcttagttat
    43201 gatcatcggc tatggtatcg ccggttggat tttatatgat tggccggctg gagtggcttc
    43261 tataccagga aacatcttcc aaaatctgat tggaatggga cttgcctttc ctataactgc
    43321 aagtcttcaa cgtattcaag cacggaagca atattaattg aaaaagaagg ttttagaaaa
    43381 tgaagttgtc agaaaaagaa ttaaaagacc aattgactga aatagttaat gacatattgg
    43441 ctgaggcaca cttgaaaaaa ggtgatcttt ttgttttagg atgcagcaca agcgaagtcg
    43501 ttggaggaca tattggaaaa aattccagtg cggaagttgg tcaatggatc atccgtacgt
    43561 taaaagagtt gttggatcct aaagaaattg cgctagctgt ccaaggttgt gaacatttga
    43621 accgagcatt agtagtagaa cgaacagtgg cagaagcaaa aaatttcgaa attgtctctg
    43681 ttgttcctgc actacatgct ggaggtgctt gttctgttgc tgcttttgac cagttcaatg
    43741 atcctgtcga agtggaacac gtagttggac aagccggtat cgatatcggt gacacatcaa
    43801 tcggtatgca tatcaagcat gtccaagtac ctgtcagacc tcgtttgaaa acccttggac
    43861 aagctcacgt gacagccctt cgctctcgtc ctaaatatat cggtggtcct agagcgaatt
    43921 attgaaatga gtaattgaca gagcaatctg aaaaacttcc tcgcaaagtc caactaatga
    43981 actttaacga ggaagtttta cttgtttgtt ttatataacc gagcttcata aggtgctagt
    44041 atgcctttag cttcaaattc tccgctagag gaatttgaca agagtaatgt ccaggactga
    44101 tctgcttctt ttggaagttc atagtcagca agtgattctg ttaagtttac cataataaga
    44161 tattgttcat cttctaaata tctttcatag acataaagtt gtggatgttc atgaagatat
    44221 tctttataag aaccgtatat cagaccttta ttggtttgtc taatgcggat catgttctta
    44281 tagaattgca ggaccgactc tggttcatat tcttgttgtt ctacattgat cgttttggta
    44341 tttggattag tgatcagcca aggttcatgt gtagaaaaac cagcatattg ttccgaagtc
    44401 cattgcatgg gtgtcctgct gttgtctctc gttgtttcac gaatgatatc caatgcttct
    44461 tctcttgtct taccttccgc aagcagcctt ttataaagtc ttttcgaatc tactgcatca
    44521 acttgatcga tcgattcaaa gggcatattc gtcattccga tttcctgtcc ttgataaata
    44581 aatggcgtac cttgcaaaaa gaaatacatc atagcgattg cttttgcaga catagcccaa
    44641 aaatcgggac gagtatctcc aaacactgaa acggcacgag gaacatcatg gttttccata
    44701 tataaagcgt tccatccgat accatccaat gaagtttgcc aagcgcttaa tgcatgtttc
    44761 aatttaagga catctaattg cccttctttt tcttgctgcc agatatggat atgatcaaat
    44821 tcgaatatca tatcgaaata accgtctttc cctacccatt gtggtccttc ttctgcagtg
    44881 acaccacttg cttctccgac agtcatgata tcgtactctt caaaaacatg tttcaaatcg
    44941 gtcaaatgtt cttcgattcc tgagacattt tgaaagggtg agaaagggtt ctctgtggct
    45001 tttactgaat attcatcttt ttttacatga gaaatcgcat ctaaacgaaa tccatctatc
    45061 cctttatcca accaccatcg gatcatattg aatagttctt ctttcaattt ctcgctttcc
    45121 caatttaagt ctggttgttc ttttgcaaaa acatgcagat aatactgtcc agtttcatga
    45181 gaatactccc aagcagaacc gccgaaaatc gatgtccaat tattcggttc tttgccatct
    45241 accccgtcga cccaaatgta ataatcgcga tacgggcttt ctttagattt tttcgattct
    45301 tcaaaccatt gatgctgatc cgaactatgg ttgatgacaa gatccataat cactttgatc
    45361 cctaattcat gtgcttcttt tagcaataaa tcaaaatctt ccatcgttcc gaatctctct
    45421 tctatatctt gatagtcact tacgtcataa ccattatcga caaatggaga ttgatagatc
    45481 ggattgatcc agataaagcc gataccaaga tctttcaaat aattaagttt ttcacggatc
    45541 ccattgatgt ctccttgacc atcgccattg ctatctttga aactagcagg atagatttga
    45601 tagcctactg cttctttcca ccatttttgt tcttgtatca tatcgtttca ctcctttaac
    45661 ggtccctctt gattataacc gattctattt tagaagtatc cttgttaccg gtaactagaa
    45721 acgaaaagct caacgaatct gctaagtgcg ttcgttgagc tttattttat catagatagc
    45781 gggcaatcag ttgaatcatt tgcttgggaa attcattcaa gtcagtaata tctacaaaac
    45841 gactttcacc gtagatcgac ttgatttctt ctttgtcttg gccaatagct gcggagacaa
    45901 ataggattcc ttgacgttca tattctttca aaacagactg gatatcttct tttgcttttt
    45961 tgcctgtata atccggcaat gcttttggct gaccgtcact aatattaagc aacagtttgg
    46021 ttgttgcgga ttgtctgttt aatttttctg ctgctaaacg aagtgcagca ccatcacgat
    46081 tgttttgcct tggtttcata gtgactaatt tttcatttaa ccattgaaat ccgtcttcaa
    46141 attctttata agaaaacaaa gaagtctttt ctcttgacga acggtctgct gtatctccgt
    46201 aaatcaacaa tgggatatcg actttcttag aaaattcggc aatggcaatc gctgcttttt
    46261 ttgccgcttc gatccgatca tcccgtatca ttgacccaga ttcatcgatc cgtacagcta
    46321 ccgctaaaga aggttgctca tgtggcgggt tctttttgtc aaaatttctc aaatcaccat
    46381 atgctacttt gcttgcattg aaccgctgac cttcgtattt tcctttttgg tacgtctctg
    46441 tttgttcatt atccagtaac tctaaaattt gccggctaag tgattctact attggcatta
    46501 cttgcatgtg tagctcacgt gcttccggcg tttttccttg gaaagctggt cggtgaacga
    46561 tgagaccttc tttttcatga atcgtttcct tcatgatttg tcgcgcttct ttattcagtt
    46621 gcttcttgat ttccttatca tgtgcttcca tgtcagaagc agacatttct tgagaatcat
    46681 ctcctgtttg ttgttctttt tgattttttc cttcgatatc gctactgcgt tctttttgca
    46741 tttgaggagg aatctcgttt ttttctattg tttcttcttg tttctcttct ccctcatttg
    46801 gttcttcagc agacaacgat ttcttttcat ctaagcgcag aatttcttca gcattttcag
    46861 tgatgtttcg ttcatttgct tcttcggcgc taatggttct ttcttttgta tgctcagatt
    46921 gaggatgacc ccatttttct tttctcaata tgtcctgaag ttccagtagc aatccactgt
    46981 cagacaaagc ttgttcaaga attgccaatt catctgaatc agtggataat ttatacaaaa
    47041 cttttggata aatcgactgg agtaaatctt gtccttgctt ataggtattt gcccaataaa
    47101 aataagaacg cattcctgct acacctttga tggcgttagc ttttgcagtt tcatccaaca
    47161 aacggataat ctctgccatc tttaagagaa gttcttgttt ttggatcata gttttcgcca
    47221 tcgcacgttc tgccatgact tctaaggcag gaagatccat tttttccgca tgttgcatcc
    47281 gatctcttaa agattcattt aatgggcgat ttccttggta atttcgattt gtagtgatca
    47341 caatgacgca atctggatgg cgacgaatga ttcctgtcgg taaattcaac atcccatttg
    47401 gttcgagcgc tgagttaagt gccacaagaa cagaggcatc tcggatgaca gtcggctctt
    47461 gaatctctaa taaatagcct ttttcaatcg ctcgaacgat ttcagaaggg taatagctgt
    47521 aatggattgc atctggataa tcagcttcga tcctttgaac tagctctgct aatttttctt
    47581 tagcagaaaa aggatcaatg ccgtaatggt tctggatcag tgtcagtaca gcttcaagtg
    47641 tatctgtttg gtaaatagct tctagtaatt cttggtcttg ttccttatta gaatctacta
    47701 caggtaataa ggctccgaaa acatctgatt tgtccatatc tgcaaaacag gtcacttttg
    47761 tatagggaag ttgcagatca gcagatagtg ctttggctaa ttgtgtcttc cccgacccgg
    47821 catctccttc caaaagtacg ttactgattt tcatttcagg atcttgccaa ttccctttga
    47881 tttcggaggc gatccgccat tcttctgcac ttgtccgatg gcttttcggt ttcttccaga
    47941 tcatttgctt ttctagccgt gaaaaggttc ggttttctgt tagtgagaaa ttctccatgc
    48001 ttctcttccc cttttgatca tctctgtcta ttaatatcct agctcatcta aaagacgttt
    48061 taaaatacgg aatcgttccc caatcagttc tccgtctttt tgtagtgtat cgtaatacaa
    48121 aatgatgtct tcatcgatct ttggattttt ggtatctact tctaccgtca ttcggttccc
    48181 ttgtaatcca atgatgtcta cgactggatt ttctggatca taaaattttt cataacgata
    48241 agcatcttgg aagtataaac ttccttgcgc agctaagatc gctaaatcta aaatacgtgt
    48301 gattggtaat tcttccgact gtcgtgaccg tttttctcca gtatgtcgcc ataccttgcc
    48361 tgagatatcg acttttccac gatcattcca ctgagcaagg cccagtgaaa gcccttttgc
    48421 atccatattt aatgcatttc ttccatcaac tttatcatag tcttctacaa cgattactgg
    48481 tttatgtttt aaatttgttg gtatttccat tattttaact cctcatcctt ttatttttag
    48541 taaataagta aattagtagt ttactcattt actaaactat atgcgacatt atagaggatt
    48601 gaagtgagga ttgcaaagaa aaagagcttc attgatagaa ataaaaacac catctcgttc
    48661 ttttacacgc tcattagtaa catgtagaaa gtccaagatg atgttggaat attt
//
LOCUS       DANKBF010000016        46199 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000016 DANKBF010000000
VERSION     DANKBF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            171..1052
                     /locus_tag="PWY89_000935"
     CDS             171..1052
                     /locus_tag="PWY89_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2065990.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1065..2675
                     /locus_tag="PWY89_000936"
     CDS             1065..2675
                     /locus_tag="PWY89_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL2065991.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2752..3411)
                     /locus_tag="PWY89_000937"
     CDS             complement(2752..3411)
                     /locus_tag="PWY89_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2065992.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3694..4359)
                     /locus_tag="PWY89_000938"
     CDS             complement(3694..4359)
                     /locus_tag="PWY89_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2065993.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4506..5111)
                     /gene="dhaL"
                     /locus_tag="PWY89_000939"
     CDS             complement(4506..5111)
                     /gene="dhaL"
                     /locus_tag="PWY89_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL2065994.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5139..6149)
                     /gene="dhaK"
                     /locus_tag="PWY89_000940"
     CDS             complement(5139..6149)
                     /gene="dhaK"
                     /locus_tag="PWY89_000940"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2065995.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6124..6570)
                     /gene="dhaM"
                     /locus_tag="PWY89_000941"
     CDS             complement(6124..6570)
                     /gene="dhaM"
                     /locus_tag="PWY89_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL2065996.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6673..6993
                     /locus_tag="PWY89_000942"
     CDS             6673..6993
                     /locus_tag="PWY89_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL2065997.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7105..7974)
                     /locus_tag="PWY89_000943"
     CDS             complement(7105..7974)
                     /locus_tag="PWY89_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2065998.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8070..9374)
                     /locus_tag="PWY89_000944"
     CDS             complement(8070..9374)
                     /locus_tag="PWY89_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2065999.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9371..9880)
                     /locus_tag="PWY89_000945"
     CDS             complement(9371..9880)
                     /locus_tag="PWY89_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066000.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10055..12190)
                     /locus_tag="PWY89_000946"
     CDS             complement(10055..12190)
                     /locus_tag="PWY89_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2066001.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12273..13928)
                     /locus_tag="PWY89_000947"
     CDS             complement(12273..13928)
                     /locus_tag="PWY89_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2066002.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13930..14244)
                     /locus_tag="PWY89_000948"
     CDS             complement(13930..14244)
                     /locus_tag="PWY89_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2066003.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14414..14674
                     /locus_tag="PWY89_000949"
     CDS             14414..14674
                     /locus_tag="PWY89_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL2066004.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14688..15002
                     /locus_tag="PWY89_000950"
     CDS             14688..15002
                     /locus_tag="PWY89_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL2066005.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15732..16286)
                     /locus_tag="PWY89_000951"
     CDS             complement(15732..16286)
                     /locus_tag="PWY89_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL2066006.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16412..18341)
                     /locus_tag="PWY89_000952"
                     /pseudo
     CDS             complement(16412..18341)
                     /locus_tag="PWY89_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18647..19363)
                     /locus_tag="PWY89_000953"
     CDS             complement(18647..19363)
                     /locus_tag="PWY89_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2066007.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19364..21187)
                     /gene="glpO"
                     /locus_tag="PWY89_000954"
     CDS             complement(19364..21187)
                     /gene="glpO"
                     /locus_tag="PWY89_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL2066008.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21201..22697)
                     /gene="glpK"
                     /locus_tag="PWY89_000955"
     CDS             complement(21201..22697)
                     /gene="glpK"
                     /locus_tag="PWY89_000955"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2066009.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22833..24293)
                     /locus_tag="PWY89_000956"
     CDS             complement(22833..24293)
                     /locus_tag="PWY89_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066010.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24283..25578)
                     /locus_tag="PWY89_000957"
     CDS             complement(24283..25578)
                     /locus_tag="PWY89_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2066011.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25910..26587)
                     /locus_tag="PWY89_000958"
     CDS             complement(25910..26587)
                     /locus_tag="PWY89_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL2066012.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26617..27708)
                     /locus_tag="PWY89_000959"
     CDS             complement(26617..27708)
                     /locus_tag="PWY89_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2066013.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27809..28243)
                     /locus_tag="PWY89_000960"
     CDS             complement(27809..28243)
                     /locus_tag="PWY89_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2066014.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28827..30284)
                     /gene="ascB"
                     /locus_tag="PWY89_000961"
     CDS             complement(28827..30284)
                     /gene="ascB"
                     /locus_tag="PWY89_000961"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2066015.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30281..32176)
                     /locus_tag="PWY89_000962"
     CDS             complement(30281..32176)
                     /locus_tag="PWY89_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2066016.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32234..34009)
                     /locus_tag="PWY89_000963"
     CDS             complement(32234..34009)
                     /locus_tag="PWY89_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2066017.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34059..35555)
                     /locus_tag="PWY89_000964"
     CDS             complement(34059..35555)
                     /locus_tag="PWY89_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2066018.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35749..37371)
                     /locus_tag="PWY89_000965"
     CDS             complement(35749..37371)
                     /locus_tag="PWY89_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2066019.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37699..39012)
                     /locus_tag="PWY89_000966"
     CDS             complement(37699..39012)
                     /locus_tag="PWY89_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2066020.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39257..39481)
                     /locus_tag="PWY89_000967"
     CDS             complement(39257..39481)
                     /locus_tag="PWY89_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066021.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39501..39923)
                     /locus_tag="PWY89_000968"
     CDS             complement(39501..39923)
                     /locus_tag="PWY89_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2066022.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40065..40775)
                     /locus_tag="PWY89_000969"
     CDS             complement(40065..40775)
                     /locus_tag="PWY89_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066023.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40793..41290)
                     /locus_tag="PWY89_000970"
     CDS             complement(40793..41290)
                     /locus_tag="PWY89_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2066024.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41327..41779)
                     /locus_tag="PWY89_000971"
     CDS             complement(41327..41779)
                     /locus_tag="PWY89_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2066025.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41914..42519)
                     /locus_tag="PWY89_000972"
     CDS             complement(41914..42519)
                     /locus_tag="PWY89_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2066026.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42969..43589)
                     /locus_tag="PWY89_000973"
     CDS             complement(42969..43589)
                     /locus_tag="PWY89_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2066027.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43591..44460)
                     /locus_tag="PWY89_000974"
     CDS             complement(43591..44460)
                     /locus_tag="PWY89_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL2066028.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44483..44890)
                     /locus_tag="PWY89_000975"
     CDS             complement(44483..44890)
                     /locus_tag="PWY89_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2066029.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44909..46198)
                     /locus_tag="PWY89_000976"
     CDS             complement(44909..46198)
                     /locus_tag="PWY89_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2066030.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12986 a         9849 c         7902 g        15462 t
ORIGIN      
        1 aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt ataaaaagaa
       61 tattatgtac taaaataata aaaaaagttt tataaaaagg tataaaacct ttttattaaa
      121 ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa
      181 tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct
      241 ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat
      301 ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt
      361 ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag
      421 gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc
      481 ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc
      541 ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg
      601 ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt
      661 tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc
      721 tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac
      781 gcgaaggaac actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga
      841 ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg
      901 actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga
      961 ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg
     1021 aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt
     1081 cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat
     1141 ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt
     1201 ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg
     1261 tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg
     1321 aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc
     1381 aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac
     1441 gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa
     1501 tatcgatcag ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt
     1561 ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg
     1621 ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg
     1681 gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc
     1741 ctttaggctg gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt
     1801 atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag
     1861 atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt
     1921 tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca
     1981 cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag
     2041 aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact
     2101 ttacaagcac gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca
     2161 tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa
     2221 tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt gctatattgg
     2281 caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga
     2341 aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag
     2401 tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt
     2461 atatctactt agcttactcc tttgcactca attactttgg cggaattttg gatttaccag
     2521 aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg
     2581 atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta
     2641 ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc
     2701 tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc
     2761 ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt
     2821 ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc
     2881 aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc
     2941 aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg
     3001 ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata
     3061 atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag
     3121 tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc
     3181 gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt
     3241 catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc
     3301 tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac
     3361 taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc
     3421 tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca actgataatt
     3481 ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct
     3541 ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt
     3601 ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa
     3661 ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt
     3721 ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat
     3781 ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt
     3841 caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag
     3901 ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc
     3961 tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt
     4021 ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca
     4081 ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt
     4141 ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag
     4201 tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt
     4261 gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg
     4321 tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat
     4381 cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc
     4441 tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat
     4501 tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca
     4561 gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta
     4621 gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct
     4681 gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct
     4741 gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct
     4801 tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg
     4861 tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat
     4921 gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct
     4981 cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat
     5041 tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg
     5101 gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca
     5161 ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga
     5221 ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct
     5281 ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc
     5341 ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa
     5401 atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag
     5461 cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct
     5521 ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc
     5581 tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca
     5641 tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac
     5701 gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc
     5761 atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg
     5821 atcagcaaaa ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga
     5881 gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac
     5941 ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct
     6001 ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt
     6061 tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc
     6121 aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc
     6181 atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac
     6241 cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa
     6301 taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac
     6361 tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa
     6421 aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac
     6481 tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac
     6541 atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt
     6601 ctaattttgg gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag
     6661 aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct
     6721 tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag
     6781 gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt
     6841 gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg
     6901 atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc
     6961 acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa
     7021 agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta
     7081 ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc
     7141 gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt
     7201 tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt
     7261 gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa
     7321 actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct
     7381 gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt
     7441 tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat
     7501 cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt
     7561 ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat
     7621 tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt
     7681 tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat
     7741 tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt
     7801 catttctgtt tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc
     7861 tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga
     7921 aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac
     7981 tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca
     8041 ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat
     8101 gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt
     8161 atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat
     8221 tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt
     8281 tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct
     8341 tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt
     8401 gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa
     8461 ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa
     8521 cgttttctgt gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc
     8581 tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct
     8641 tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc
     8701 gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg
     8761 atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt
     8821 tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa
     8881 ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc
     8941 aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt
     9001 tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt
     9061 gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc
     9121 gtcatcaaat actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg
     9181 atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg
     9241 tataaaaaca cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa
     9301 gaaacgagaa aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg
     9361 gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg
     9421 tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg
     9481 ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat
     9541 cttcatattc aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca
     9601 atacaaatga ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat
     9661 acagcatttt cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc
     9721 tcgttaatgg gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag
     9781 ccactaacgt ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca
     9841 ccaaaattga tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt
     9901 tgttacgacg taggagttta ccatacattt catttctttt cacataaagt gttcctaaat
     9961 ttttcctaag aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt
    10021 ttatcagttg ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa
    10081 tgcttttcat tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc
    10141 catcgttttc aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca
    10201 aaggaatgat ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg
    10261 tttgggtttc ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca
    10321 aaatctcagc tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt
    10381 cttttccttg cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt
    10441 tgaaaagctc tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga
    10501 tcggcagatg gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa
    10561 agattgcctg attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca
    10621 atagacctaa ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg
    10681 ctggtacatt taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat
    10741 caacagtacg tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt
    10801 caaacaactg atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat
    10861 attttttttg taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa
    10921 tcaatttacc gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca
    10981 cagcaggact ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt
    11041 tcgttgcttg aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt
    11101 cgatcgtttt tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg
    11161 gatgatctaa aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca
    11221 aacgttctgt ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac
    11281 cttctatttc ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat
    11341 ttctttcacc taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg
    11401 atagaatcag caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa
    11461 tacgctgcat gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt
    11521 gacttttcac atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct
    11581 tcatttcatt ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta
    11641 cagacgtatc aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt
    11701 taataaccgt atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat
    11761 tgtaaaaaag ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat
    11821 ctgtgaaatt tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta
    11881 caacgacttt tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt
    11941 ctgcgttttt ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg
    12001 aaaaagtatc caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt
    12061 ctttcaacga ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt
    12121 ttttatcaat tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg
    12181 tgttgaccat acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt
    12241 ttaagagtcg aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca
    12301 ggtcggacag tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac
    12361 tgcttcaaga ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat
    12421 tcttgttggc tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg
    12481 gcttccttga atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt
    12541 ccttttgcta gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca
    12601 atgttcattg ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt
    12661 gcatacgtca attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt
    12721 gctaaaatat cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc
    12781 tcgccagtct ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag
    12841 cttgcatgat cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct
    12901 gttctttcac ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt
    12961 ccaataccgc cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct
    13021 tgacgattgg ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt
    13081 acaacgatag catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct
    13141 actaggatac ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca
    13201 ggacgaacac caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt
    13261 cgcaatcgtt tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact
    13321 ccttgatgag acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga
    13381 atatgtggtg cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc
    13441 atttccaatc cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat
    13501 gcatgcatca ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct
    13561 gttatccctg gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg
    13621 tactgttcaa tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag
    13681 atggctgtca cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct
    13741 ggcgtttgga caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag
    13801 ccgcaatttg caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc
    13861 attaatcggc gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg
    13921 attttcatct tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac
    13981 attcactaca tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc
    14041 tgattgacaa atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg
    14101 taatggaaca tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac
    14161 atcaagtaac gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt
    14221 tattgattga aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca
    14281 tggggtatat taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt
    14341 cactactttt tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag
    14401 aggagaatgt gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga
    14461 gggacaaatt cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat
    14521 cactcaatta agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga
    14581 taatctgaaa aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact
    14641 agaacaagcg atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt
    14701 tccatattga tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa
    14761 aagcttctcc agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc
    14821 tagactctgc caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca
    14881 atgctatgcg tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac
    14941 cagcaggtgt ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt
    15001 agtcagtaga ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa
    15061 gaagctactt ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta
    15121 ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg
    15181 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta
    15241 agtattagag aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata
    15301 ccgaaggtca gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt
    15361 cgagtattaa gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta
    15421 ataccgagaa actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg
    15481 acctgagtat tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc
    15541 taataccgaa ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta
    15601 actccgaatc acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct
    15661 tattatcgag aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc
    15721 acaccccttt tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa
    15781 gattgtaaaa gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat
    15841 tctgggaaaa gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca
    15901 taccacttcg ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac
    15961 ataaagcctt cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat
    16021 gccatagaca aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc
    16081 cctaagtctg ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa
    16141 cctgagacaa caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga
    16201 taaatcggca gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct
    16261 ttacgccagt ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt
    16321 ttattttagc ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca
    16381 gacactgata ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc
    16441 tttcttcctg tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc
    16501 gctctaggtc acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt
    16561 aacctgtcgg gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt
    16621 ggattatttt tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta
    16681 acgcttcttt ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt
    16741 cttttttatt ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg
    16801 tccgataggc tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat
    16861 ccattttatg agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga
    16921 tatttaggac gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga
    16981 tctccggcga atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc
    17041 tcatacatag attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca
    17101 ctgccaatct catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg
    17161 atgttttgca tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg
    17221 acatcgacga aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc
    17281 ataccgaaca tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct
    17341 ccggcatttg ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat
    17401 ggtaccgcat gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat
    17461 ttattgattc taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg
    17521 atatctaaca ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata
    17581 gcaccagtca atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac
    17641 tgatagtgtt cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta
    17701 tttgattcat cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc
    17761 gtatagtatt cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga
    17821 tgacgcaaga atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg
    17881 cgtactttac gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg
    17941 tcaatcaact cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag
    18001 ggtgtctggt attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa
    18061 tccgcatatt cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc
    18121 agtgcatcga tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag
    18181 cgtgcttctc ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc
    18241 atatcgctga ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg
    18301 atccgatcaa attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa
    18361 gagatacctg tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct
    18421 taaaaaagtc atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc
    18481 gtttcataag cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac
    18541 cagctacact cacaaaacat accccattct tctaggtctt atttattttc cacttaacta
    18601 tagcaagaca cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg
    18661 aatagacacc taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat
    18721 atgcccaatc tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac
    18781 caaagtcccg agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg
    18841 ctaagatcaa tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa
    18901 aagctagcaa cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg
    18961 gataattccg tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg
    19021 ttgcattcca atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta
    19081 aaacttgcgc aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca
    19141 tagctagaga gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag
    19201 ccattgttac agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt
    19261 tacttttgtt taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg
    19321 tcccaataaa ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta
    19381 gttcccgcag atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct
    19441 cctcttctgt ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat
    19501 caagctgatc tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg
    19561 ccaaaaccat ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac
    19621 caggataagg tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa
    19681 aatcagcgat ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta
    19741 attctgtcac tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt
    19801 ttttagagtc gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca
    19861 atcgcaaagc tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca
    19921 gcaaaccatc tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt
    19981 catcacggct gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt
    20041 tttccttata ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc
    20101 cattatctcc accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg
    20161 cccagcttgc ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca
    20221 catccaatag ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt
    20281 aatccgtatc tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc
    20341 gcccatcttg ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat
    20401 ctacaactaa atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat
    20461 tcaagtttcg tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga
    20521 tttcgatcac ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat
    20581 catataaaaa gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct
    20641 ttttgatatt atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg
    20701 cgccttttag cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca
    20761 gcgtataatt ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg
    20821 ctactttgac cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa
    20881 gcattggatc tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa
    20941 ctgtgtcagc tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa
    21001 gtttggtcga gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt
    21061 tgattcctga tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga
    21121 tcaacagatc caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg
    21181 aaaacatatg gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa
    21241 ccgttgtaaa gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct
    21301 tcataaaatt cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa
    21361 aatgctgccc ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag
    21421 atatctgttt ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat
    21481 acaggaatct tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa
    21541 gcaatcgact gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca
    21601 aacatagccc ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga
    21661 acaacataaa cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct
    21721 ttttgcaaca tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata
    21781 cttccttcta aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa
    21841 ttatttttgg ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca
    21901 taagtattct tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg
    21961 tctcctgcca tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa
    22021 ccataaactt ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa
    22081 tctaaaatct cctgatccca atctagctga tggatattga acaacatcgt ccgactggca
    22141 ttcgagtaat cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg
    22201 atcgttccaa acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca
    22261 agaatccagc gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg
    22321 tggaaaaatt cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg
    22381 gattgccaaa caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt
    22441 gtttcacgct ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg
    22501 atgaacgctc cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc
    22561 acccaacttt cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt
    22621 cctttcttgt caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata
    22681 tacgttgatt ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc
    22741 ttatcatact actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt
    22801 tgacagtttt ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct
    22861 attcttcttt tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca
    22921 cactgttttt tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca
    22981 taacttccaa tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa
    23041 gcaatacgat aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata
    23101 taaaaatcga tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta
    23161 tcttcagctc ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac
    23221 tgtgttaagg aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag
    23281 acttcgatct ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct
    23341 actgcctttt cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc
    23401 cgaatgtaag tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga
    23461 taaaatattt cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt
    23521 tcgtatacat tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt
    23581 gcttgataaa gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta
    23641 cttttttcat tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct
    23701 gctgatgccg taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt
    23761 tctggtgtat tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca
    23821 taataaaaat atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat
    23881 ccaaattcct ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg
    23941 atattaaacg ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg
    24001 agcatcttaa acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca
    24061 tcttcgtcca acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc
    24121 atcattcggc caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt
    24181 tggacatgct ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc
    24241 aaaatcagga tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt
    24301 attcgccacc tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc
    24361 atgttcttct aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag
    24421 aaggtggaac atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc
    24481 aaatagaaat ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta
    24541 tcctgtttca attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc
    24601 taagaaatag ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg
    24661 tgagccacta attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga
    24721 aacatacatt ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata
    24781 aacgtctttt tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa
    24841 tgttaattgt tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag
    24901 atcatagttc tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga
    24961 cacaacttgt tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg
    25021 ttctgtcatt tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg
    25081 gatttgtttt tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc
    25141 aataattgca attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat
    25201 ctttttaact gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga
    25261 accagtcgct agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt
    25321 tttttctttc aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt
    25381 cagttcttct ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat
    25441 caaattgaac ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc
    25501 acttgttggc atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc
    25561 gacaattaca atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct
    25621 caatcagaaa acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc
    25681 agaatcaacc ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg
    25741 acctaattga agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga
    25801 aaaaaactcg ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg
    25861 agttttagcg tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta
    25921 atgcttcaac aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa
    25981 gccgcccatc aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt
    26041 tttctttcaa agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct
    26101 cttcttcatt actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat
    26161 ctgtaactgg caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc
    26221 gatagatttg tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc
    26281 cgtggccacc agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta
    26341 aggtatttcc caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt
    26401 catccatatt caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa
    26461 aatctgcatc gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc
    26521 acaaacctgt aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata
    26581 ctttcatttt cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa
    26641 attatataat gggcttggca aataaagctc tttatatgct ttttcaaaag gtttcgttac
    26701 aaattcatcc tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg
    26761 ctgtccaatt gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg
    26821 gatcccaccg acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag
    26881 aatcgattta tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac
    26941 atgcagataa tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa
    27001 acggatacct ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt
    27061 tttagcatat tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata
    27121 acgattttcc aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa
    27181 ttgttggatc aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat
    27241 cgctctacgt gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat
    27301 tgctcgtggt gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg
    27361 ttctccagat aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc
    27421 ttttgcgcct tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc
    27481 atgagcgatg ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc
    27541 agtaataaca gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt
    27601 gatcactcca ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt
    27661 caattgaact cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc
    27721 cataattcgc tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg
    27781 tacatgcaac taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat
    27841 tcattgtctg ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat
    27901 gaagttcttc ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat
    27961 aaactaatga caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt
    28021 caacaaaacg agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg
    28081 gtattccatc tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg
    28141 tccttctaaa cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa
    28201 agaaaagaca ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg
    28261 cgtacatatc atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc
    28321 ctctgtctag atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga
    28381 caaaactcct tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt
    28441 atgaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc
    28501 gaaaaactgg cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca
    28561 agtaataaga acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg
    28621 ccgatggtca gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc
    28681 gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt
    28741 gatcgaggag ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt
    28801 ttgtcacagt cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt
    28861 ctgtaccaat caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct
    28921 tgatcaacat agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata
    28981 tcaatgcagc cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc
    29041 atctgctcaa tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct
    29101 tcttctactt tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac
    29161 ctatcatata attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat
    29221 tcacttgatg gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct
    29281 tcccaatttt ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa
    29341 tcaacagggt aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct
    29401 ttttcttcaa acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta
    29461 aagaacaaag aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa
    29521 tacggaacaa gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca
    29581 tgacaagctt ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc
    29641 gcttgttcca aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt
    29701 tttacacagt tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg
    29761 aatactgttt cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca
    29821 tattctgtaa ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc
    29881 ccatatttta gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt
    29941 gtttcttcat caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta
    30001 aaacccattt cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg
    30061 tgtcttttag gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc
    30121 gtcatcatat gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac
    30181 acatcagaca cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc
    30241 gctgtcgctc cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac
    30301 ttgctagttt tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac
    30361 tactatttgt aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca
    30421 aatctacttc catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt
    30481 gaaagtattt gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc
    30541 cttcttcact gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt
    30601 ttacagaact gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc
    30661 cactagcaaa tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag
    30721 tacttaacac tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa
    30781 aagttagcat acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc
    30841 cttcaccaat ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg
    30901 ctcctgcggc accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg
    30961 gtcgtttata tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg
    31021 tgataaatgc agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc
    31081 caagtactgc tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac
    31141 gaccgtagaa agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa
    31201 tcactaacac ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat
    31261 aaacagctaa atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa
    31321 ctaataaaat aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa
    31381 tactttcgtg caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta
    31441 aaattgctgg aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc
    31501 gcaaggttat gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa
    31561 taaccatagc gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata
    31621 taggcaagaa atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt
    31681 cagtagttaa taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc
    31741 ctgccaagac gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta
    31801 aaatgccttt tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt
    31861 gtttggataa tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc
    31921 ctccagcttc gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct
    31981 tcttattatc ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat
    32041 tttcttcgcc tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta
    32101 cttccccctt gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag
    32161 aaaacggttt ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt
    32221 gtgtatggtt tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt
    32281 ttctcttata gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa
    32341 gtggtatctc aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc
    32401 acaacaaagt ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt
    32461 gctgaatgga atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat
    32521 caagcagttt tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat
    32581 aaatctccca agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact
    32641 caaaaataag ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt
    32701 gatttttgaa aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt
    32761 gttgaatctg tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta
    32821 tctccccttg ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc
    32881 tttcagctaa attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa
    32941 tttctaaagg ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg
    33001 catccatcct cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca
    33061 ataacgaaac aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct
    33121 tctctatttc tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat
    33181 tttcaattgc ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt
    33241 ttgtgagggt ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt
    33301 cttctcttcg aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg
    33361 aaacaatata attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga
    33421 tattggaaaa aattgtactg atttgttgag gaaaccttgc gtgttggatc accgtttctg
    33481 ttccaacaag agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt
    33541 ttgtcgacga aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt
    33601 ttatttgtaa tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac
    33661 tgacataccc tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc
    33721 tggaatagat tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa
    33781 ttgattctaa aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga
    33841 tagcaagcat atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag
    33901 ctaattcttc gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt
    33961 gtcgtttatt taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac
    34021 ctctcaaatg acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat
    34081 tcaaggtttt taccattaga ctggataatt tgtttataat attcaaaact atcttttttc
    34141 aatctcctaa agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc
    34201 ttcctcattt cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca
    34261 atgatttggc aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac
    34321 tcacaacgtt ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg
    34381 ccattttcaa ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa
    34441 cgaaacccta gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag
    34501 ggattatcga aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag
    34561 acagcgctac gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt
    34621 tcgtctccat ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa
    34681 gctggataat gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc
    34741 agtgaaccaa atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag
    34801 ctacacataa tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg
    34861 gcgttcgcta gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca
    34921 atgggttcaa gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata
    34981 cgaaaagcat tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt
    35041 tcaaagacgg tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc
    35101 ccatactctc ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg
    35161 atatcatatc gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt
    35221 ggcttctgtt cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct
    35281 tttaaaccta actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc
    35341 gcttcatgag aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct
    35401 tcattccact gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta
    35461 ggtaatttgc cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc
    35521 cataaaaaat tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa
    35581 tttcttactt gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga
    35641 agagaagaaa aaaattgaat gctactcttc atttttaata tttatatata acaaatacat
    35701 aagacgatcc ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc
    35761 caacacaaat gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg
    35821 ataatttgac acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact
    35881 ggacatatta caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac
    35941 atgctcaggc gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct
    36001 acgcaaatcg atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc
    36061 ttctgcattg atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa
    36121 cccagcatgt tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc
    36181 cttgattgat tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat
    36241 attgattgtc tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc
    36301 taactcttct ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa
    36361 catcttggca ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg
    36421 aagatcgtga ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca
    36481 tttggataat acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt
    36541 gccttcttgc tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct
    36601 tttaggattg gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt
    36661 catcagattt ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt
    36721 aggtccatta cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat
    36781 acgaaagccc ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg
    36841 aacctgttta tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata
    36901 ttgccccgtt ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt
    36961 aggctctttt ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt
    37021 atttttacgg gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc
    37081 catgatgata cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat
    37141 cgtaccgaat tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt
    37201 cggagactcg tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc
    37261 caacctggaa ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact
    37321 tcttggatag atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct
    37381 cctataaatg gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa
    37441 attgtaacgc tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa
    37501 acagctgagg aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg
    37561 ctgatttcac aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa
    37621 aaaacggcaa aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac
    37681 gctgatttca caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa
    37741 gaacagacca tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat
    37801 tggatagaag ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc
    37861 gatcaagatc aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa
    37921 agattgtgcc atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac
    37981 aactgatagt aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc
    38041 tgtgatacct gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac
    38101 aggagaagta ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt
    38161 ccctacacga ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa
    38221 agttcctgct gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg
    38281 aagagaattg atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg
    38341 tgctgcggca acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt
    38401 tgtagctgcc atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt
    38461 tacgactaat ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac
    38521 taatgtagat tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa
    38581 tagaccgata ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat
    38641 ttcccgcagc ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc
    38701 taatgctgtt tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt
    38761 gatcccaaga gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca
    38821 gccgatcgca ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc
    38881 acctaataca gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc
    38941 tgccagtacc tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat
    39001 tttttccttc atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt
    39061 tcgatttatt aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa
    39121 aaaattcgga ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa
    39181 gttcatgaac attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc
    39241 attctagtct caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt
    39301 tgatgaattc ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga
    39361 ccatttttcc ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct
    39421 tctcactatg ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca
    39481 ctgtttttcc tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg
    39541 ccttcagggt cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt
    39601 tcttccatgt tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt
    39661 ggtcctaata cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct
    39721 aattcttttg agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca
    39781 gaaatttctg caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca
    39841 taaagcatca cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt
    39901 tcctgtctat aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact
    39961 attcaagaaa aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca
    40021 aaaaaaaaac ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct
    40081 gattcatctt tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt
    40141 tccttcaacg cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca
    40201 atgaccagca ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag
    40261 ttgagacagc gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc
    40321 ggagcatcgg aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa
    40381 tgcagatttc ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag
    40441 gaaatactat tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt
    40501 gcacgtttca gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta
    40561 aatgcctgac gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa
    40621 tttcttgctt cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt
    40681 gttacgtggt tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact
    40741 tcccagaaat tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata
    40801 attgcttgat gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc
    40861 tgtatttgat aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa
    40921 caccgagcca aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta
    40981 aaaatgtacc gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta
    41041 aaaaatgatc agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg
    41101 caggctcttg atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg
    41161 taagtttttc tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat
    41221 cttttccaaa tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg
    41281 aaggcgtcat ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt
    41341 tattgccttt tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa
    41401 gatattccaa ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt
    41461 agttgttttc tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt
    41521 tcttgatttt tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt
    41581 tcttgattgc tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt
    41641 ggatgattcc aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat
    41701 cgacttcttt gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt
    41761 ctcgaaggat ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc
    41821 tttttgttgc aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt
    41881 aaagtatatt cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt
    41941 ttctaaaata agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc
    42001 tatactaaaa aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc
    42061 gtggatcgca caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg
    42121 tttattcgta tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc
    42181 atagacttct ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg
    42241 aagtgaccat tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat
    42301 ataggcacga acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt
    42361 tatttcttcg aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat
    42421 ctcctcaaaa cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag
    42481 tgcgagtagt cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc
    42541 aaatgtatga ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa
    42601 tgaaatctgc aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc
    42661 tctggggctg tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa
    42721 acgaaaaata tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct
    42781 ttttcacaac ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa
    42841 tattcatcaa acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac
    42901 gacaggctca cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca
    42961 tttctttgtt acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa
    43021 tccacttgag tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca
    43081 tattttcggt cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc
    43141 gttcgatctt ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga
    43201 tctgattcac gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg
    43261 cgaatcgtga tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc
    43321 ttatcactaa aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca
    43381 agtgctttgt aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa
    43441 ttgttaaacc cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac
    43501 atatctggaa atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta
    43561 ccagccagaa gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc
    43621 caagttcata aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg
    43681 aatcacatta tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac
    43741 tttcgtccag ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat
    43801 caattcagga taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc
    43861 aactactgca tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg
    43921 tccatgattc gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt
    43981 cactgcgtct tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc
    44041 tgtaccgccg attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc
    44101 tactactgct tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat
    44161 gacagaagta tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc
    44221 taagtgcaag actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa
    44281 cacatgcatg ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc
    44341 catttctttt gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc
    44401 tccaactgta aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat
    44461 cttttattgc caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt
    44521 gtcgcatctc atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag
    44581 cagcatctgc acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg
    44641 tctgtttatt ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt
    44701 cagaaaattt aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag
    44761 tggaaatctc ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg
    44821 atagggcttt ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa
    44881 atccaaacat cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc
    44941 aggcatataa gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgttgaatc
    45001 ccatcgaatt aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag
    45061 caataccgcc aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg
    45121 tccctccggc aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg
    45181 gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag
    45241 catttgggct ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta
    45301 atttcatagc tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag
    45361 ctagcaatac atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat
    45421 agttttgatc tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga
    45481 tatatagcgg gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat
    45541 gtgtcggcaa cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct
    45601 tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat
    45661 ttgatccagt gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac
    45721 aagctccgat aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc
    45781 caaagactgt cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact
    45841 tccattttgt gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg
    45901 caatatctct acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag
    45961 ggatgatccc ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc
    46021 caacgatgat gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc
    46081 cagagatgac ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg
    46141 caatggtgat cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatg
//
LOCUS       DANKBF010000017        45667 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000017 DANKBF010000000
VERSION     DANKBF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            4..243
                     /locus_tag="PWY89_000977"
     CDS             4..243
                     /locus_tag="PWY89_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066031.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            310..519
                     /locus_tag="PWY89_000978"
     CDS             310..519
                     /locus_tag="PWY89_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066032.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            579..1250
                     /locus_tag="PWY89_000979"
     CDS             579..1250
                     /locus_tag="PWY89_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2066033.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1303..3279
                     /locus_tag="PWY89_000980"
                     /pseudo
     CDS             1303..3279
                     /locus_tag="PWY89_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
     gene            3292..4047
                     /locus_tag="PWY89_000981"
     CDS             3292..4047
                     /locus_tag="PWY89_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2066034.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4044..4868
                     /locus_tag="PWY89_000982"
     CDS             4044..4868
                     /locus_tag="PWY89_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066035.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5169..7268
                     /locus_tag="PWY89_000983"
     CDS             5169..7268
                     /locus_tag="PWY89_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2066036.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7306..9363)
                     /locus_tag="PWY89_000984"
     CDS             complement(7306..9363)
                     /locus_tag="PWY89_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDL2066037.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9477..9689)
                     /locus_tag="PWY89_000985"
     CDS             complement(9477..9689)
                     /locus_tag="PWY89_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066038.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9876..10343
                     /locus_tag="PWY89_000986"
     CDS             9876..10343
                     /locus_tag="PWY89_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066039.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10340..10582
                     /locus_tag="PWY89_000987"
     CDS             10340..10582
                     /locus_tag="PWY89_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDL2066040.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10600..10902
                     /locus_tag="PWY89_000988"
     CDS             10600..10902
                     /locus_tag="PWY89_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066041.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10974..13028
                     /locus_tag="PWY89_000989"
     CDS             10974..13028
                     /locus_tag="PWY89_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066042.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13028..15640
                     /locus_tag="PWY89_000990"
     CDS             13028..15640
                     /locus_tag="PWY89_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2066043.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15637..18168
                     /locus_tag="PWY89_000991"
     CDS             15637..18168
                     /locus_tag="PWY89_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066044.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18220..18552
                     /locus_tag="PWY89_000992"
     CDS             18220..18552
                     /locus_tag="PWY89_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066045.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18553..19146
                     /locus_tag="PWY89_000993"
     CDS             18553..19146
                     /locus_tag="PWY89_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066046.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19309..21003
                     /locus_tag="PWY89_000994"
     CDS             19309..21003
                     /locus_tag="PWY89_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2066047.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21691..23568
                     /gene="ltrA"
                     /locus_tag="PWY89_000995"
     CDS             21691..23568
                     /gene="ltrA"
                     /locus_tag="PWY89_000995"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2066048.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23699..24076
                     /locus_tag="PWY89_000996"
                     /pseudo
     CDS             23699..24076
                     /locus_tag="PWY89_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24076..25281
                     /locus_tag="PWY89_000997"
     CDS             24076..25281
                     /locus_tag="PWY89_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2066049.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25297..25941
                     /locus_tag="PWY89_000998"
     CDS             25297..25941
                     /locus_tag="PWY89_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066050.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25952..26758
                     /locus_tag="PWY89_000999"
     CDS             25952..26758
                     /locus_tag="PWY89_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066051.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26780..27010
                     /locus_tag="PWY89_001000"
     CDS             26780..27010
                     /locus_tag="PWY89_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066052.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27556..27831
                     /locus_tag="PWY89_001001"
     CDS             27556..27831
                     /locus_tag="PWY89_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066053.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27873..28295
                     /locus_tag="PWY89_001002"
     CDS             27873..28295
                     /locus_tag="PWY89_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066054.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28314..29906
                     /locus_tag="PWY89_001003"
     CDS             28314..29906
                     /locus_tag="PWY89_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066055.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29918..30253
                     /locus_tag="PWY89_001004"
     CDS             29918..30253
                     /locus_tag="PWY89_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066056.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30400..30594
                     /locus_tag="PWY89_001005"
     CDS             30400..30594
                     /locus_tag="PWY89_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066057.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30626..32998
                     /locus_tag="PWY89_001006"
     CDS             30626..32998
                     /locus_tag="PWY89_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066058.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33059..34519
                     /locus_tag="PWY89_001007"
     CDS             33059..34519
                     /locus_tag="PWY89_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2066059.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34530..36734
                     /gene="topB"
                     /locus_tag="PWY89_001008"
     CDS             34530..36734
                     /gene="topB"
                     /locus_tag="PWY89_001008"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2066060.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36750..36899
                     /locus_tag="PWY89_001009"
     CDS             36750..36899
                     /locus_tag="PWY89_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066061.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36973..37566
                     /locus_tag="PWY89_001010"
     CDS             36973..37566
                     /locus_tag="PWY89_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL2066062.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37582..38481
                     /locus_tag="PWY89_001011"
     CDS             37582..38481
                     /locus_tag="PWY89_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL2066063.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38484..38969
                     /gene="ssb"
                     /locus_tag="PWY89_001012"
     CDS             38484..38969
                     /gene="ssb"
                     /locus_tag="PWY89_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2066064.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39335..39595
                     /locus_tag="PWY89_001013"
     CDS             39335..39595
                     /locus_tag="PWY89_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066065.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40036..40242
                     /locus_tag="PWY89_001014"
     CDS             40036..40242
                     /locus_tag="PWY89_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL2066066.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40242..40493
                     /locus_tag="PWY89_001015"
     CDS             40242..40493
                     /locus_tag="PWY89_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066067.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40508..40945
                     /locus_tag="PWY89_001016"
     CDS             40508..40945
                     /locus_tag="PWY89_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066068.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40938..41645
                     /locus_tag="PWY89_001017"
     CDS             40938..41645
                     /locus_tag="PWY89_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066069.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42026..42205
                     /locus_tag="PWY89_001018"
     CDS             42026..42205
                     /locus_tag="PWY89_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066070.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42546..42809
                     /locus_tag="PWY89_001019"
     CDS             42546..42809
                     /locus_tag="PWY89_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDL2066071.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42803..43153
                     /locus_tag="PWY89_001020"
     CDS             42803..43153
                     /locus_tag="PWY89_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2066072.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43177..43791)
                     /locus_tag="PWY89_001021"
     CDS             complement(43177..43791)
                     /locus_tag="PWY89_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2066073.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44105..44527
                     /locus_tag="PWY89_001022"
     CDS             44105..44527
                     /locus_tag="PWY89_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066074.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44537..44740
                     /locus_tag="PWY89_001023"
     CDS             44537..44740
                     /locus_tag="PWY89_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066075.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44951..45535
                     /locus_tag="PWY89_001024"
     CDS             44951..45535
                     /locus_tag="PWY89_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2066076.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16742 a         7204 c         9260 g        12461 t
ORIGIN      
        1 tagatggcgc gaccagtttt agaggcgatg gatatggata ctttgacaaa aggaaggtat
       61 gtacagctgt tagctcgtgg ttattcacct aaagaagtat tcaaagcctt atctaaagga
      121 acagatatgg aaaaagaaag attgcgaaaa gaattcgatt attggagaac ccataatgga
      181 atcaaggatt tgaaaccagc acggccagta attaaacgaa agaaaaagat aacccaagac
      241 tgaagtgtag agcgtatgtg atcataggct tttttatttt tgcaaaaaac aaggaaggta
      301 ggaaaactca tgaattatga agaaatcgaa aacagaaaaa aagtgtcgaa agaaatggaa
      361 gaaaagttac taaaaacgat gaagcaaaaa caccttaaaa gattatctgt cgcgcaatat
      421 atcaatgata tgcagttaac aggaaaagag aaagcttgtt tgttaggttc aatgaaaaac
      481 tttgaacagt tgagacgaac ttatagaatt catttttaaa actttttcta tggaaatgag
      541 aaaaagtttt tttgttttgt ctgaaaggag gcagatggat gaaaaaatgg ttatttggtt
      601 ttttaggagt cactttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac
      661 atgaaggaga ggtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg
      721 cacatgcaaa gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc
      781 aaatgttgtc catacaaaaa gaagcaatcg aaatgggcgt caataaacaa gtatttgcac
      841 aaattcagat tcctgcactt ggtttagcat tgccaatttt taaaggggca aatcaataca
      901 ctttgagtct tggagcggct acttattttt atgaagatgc cgaaatgggc aaaggaaact
      961 atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat attcaaaagt
     1021 tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa
     1081 cgcgtaaatt tatcgtacct gggtacttta aattaatcga tggcgttcct gaagagaact
     1141 ctttcttgtc tttgccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt
     1201 atacgtcaca aggaaaagaa agatacgtgg tacagggaga gctacaataa caaggtttta
     1261 atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggtat
     1321 gtaaaaaaag ctggaatgct tgttggaatg ttaggaatca ttgcaggctg ctttcagggg
     1381 tatcaagtac atgcagaaga tgtcacgcaa aaaacaccac cagagaaggt caatatcacg
     1441 gttcataaat taatgtacga tcaaggaacg caattgaatg ttgatattga cgggatcaaa
     1501 aacgatggtt atacgcacga tcaatatcct acaggtgtaa cgaaatacaa caaggcagat
     1561 tatggtgatg ttgagtttac gttaggaaat attacggatc aagtattacc gagagaagat
     1621 tctgatctaa ccaacgcgaa agtggacgag attgtacaag atgtagaaga caagggatct
     1681 aacagtgagt acgtcaagaa tgcaaccaac aaaattactt cagctgtaga tgaaaatggt
     1741 gagatcactt ttactgatca accagcctat gtcaattcca aagggaatgt atatgtggtt
     1801 tatgaaagta aatccgctgc tggattagtc acacaaaaag caaaaccaat ggttgtgatt
     1861 gcgccaatga cagataaaac aggatcagga tttttaaaaa atattcattt gtatcctaag
     1921 aacattgttt ctaagttgag ttttgaatta acgaaatttg gtgacgatgg cgcagaccaa
     1981 agcaaacaaa cccttttaaa aggggcaaaa tttgagttat acaagggcga acctggaaaa
     2041 ggaacaaaat tgggtgatct tgtatcggat gatcaaggaa agctgactgc gactgatttg
     2101 acgttaggaa aatactattt tgtggaagtt ccttcggaag ttgtagtggg atcagatcaa
     2161 gaaccaacag cagatcaata tttattaggc gcagacgctc gcaacgatgc gcacaataaa
     2221 ttaacctttg aaattacgaa tgatggcgtg acaagtgact taaaagcttc atacgtaaac
     2281 tataaagcac cagtgatcga taaaacagta acaaatggaa caggacaaga acattctttc
     2341 caaattggtg acgcagtaaa ttatcaagga acgattcata ttccaacaga cattgcaggt
     2401 ggcgcagacg gtatcacagt aaacggtgtg aaaagcgaaa cttctcctta cagtgtattt
     2461 aaatggggag atacagcagg taaaggactt tcttatgttg ctgaaaaagc agatattaaa
     2521 gtaacaaata atgatggttc agtcgtatta aaagaaaata ctgactataa aatcgaaaat
     2581 tctgatagtg gttttgtgat cgattttatc gtaaataatg gacaagtaag tgatacagtt
     2641 gcgaatttac atggtcaaga tctaaaaatg acttataaca tgtttgtgaa tgatagtgct
     2701 gaaatcgcaa atccattaac aaatagcgtg gatttcgttt ataacaataa tccatttaat
     2761 caggaagaac accacgaaaa aacgaaagct gacgttgtga cttatggcgc aaaattctta
     2821 aaagtcgata gcggcctatt tggaacaggt atcaaagcaa caccgcttga aggcgcagaa
     2881 tttgcagcta agaatgcaga aggtaagtac tacggtggac ttactgatac agataaagat
     2941 ggtgtcaaag aagcagtttg ggtagacgat gtagcaaatg cagcaatcct aaaataagat
     3001 aaagagggtc attttgaaat cactggttta actgaaggag attactcact ggaagaaaca
     3061 aaagctcctg aaaactatca aaaattaaca aaagagatct ctttcaaagt agacaaagat
     3121 tcatacaaag aagaaaaccg aatcacgatc aaaaataatc aaaaagcttc cgtaccaatg
     3181 actggtagca acggtttcca atcttacgta ttgatctctt gcttgttgct aggagcaggg
     3241 ggactttctg cagttgttta ctttaagaag aaagcctaga aggatcagct aatgattatt
     3301 cgtaagcccc caaaaagaag agtcatgcac aaactgatga tatttttttg gttgctgtcg
     3361 gcagtagcag tctatttaat tccagttgtg gaaacaaatg tagcgaaatt tcaacaacag
     3421 caaagaattt tagccattga aaatcttgaa aaaacagatt cttctttgga tgttgcgaaa
     3481 atagagttag gcgatcctgt tggtgttctt acaattccaa gtatttcttt aaaactacca
     3541 atttatgacg gaacttccga caaaatttta gagaatggtg tagggattac ggaaggaact
     3601 ggcgatatta caggaggaaa cggtaaaaat cctttaatcg cggggcacag tggactttat
     3661 aaagataatc ttttcgatga tttgccttca gtaaaaaaag ggcaaaaatt ctttattaag
     3721 gtaaatgaag aacaacatgc ctatcaaatt tatcgaattg aagaagttaa agcagaagaa
     3781 ttacaaaaaa attatttaga ttatcttatg cctgaaaaag accaagatag gatcacgtta
     3841 atgacatgca caccaaaagg tgtcaactca caccgctttt tagtgtatgg tgaacgagtt
     3901 tccttcagta aagaagattt aaaactacag aaaaaagcaa aaaataggaa atcaatttca
     3961 ttggattggc gattagcact gctattcgct tttttttgcg ttcctattat ttttatttat
     4021 aaaaaaataa ggaggagatc ggaatgaata agagaaaatg gctgtttgtt tgtgtagcag
     4081 ctctcttttc tactgtagct tattcgcaac tgtctttcgc tgacagcagt gatagtagta
     4141 ccactcaaac aatagataat tcaattactg caccaagcga aacggacagt tcggagaata
     4201 ctacagaaga aacggcagat tcaaatataa gctttactga atcaacgaat ccaacgtcag
     4261 aaagttcttc agagacttca gaaaagccgc aagacgtaga agatacgcaa gtgatcaagc
     4321 aagccattca tattcaaaaa attatttctt cgaatcaaat caacaatcaa ggcagtcaag
     4381 tagcccagca agaaggtatc aatggcgcga agtttattgt ctacgatatc acgaatatct
     4441 tagaagaaat gtcacaagat gataatgaga aaaatcaatc tgtagaaata attgagtcaa
     4501 aattgaaaga tcgagcaaaa aaactttcat acgaccagtt gaaaaaagtg gctgaaggag
     4561 aaaccaaaac gattgatgat caggaaggcg tgattgaatt ttccattgaa gtacctgcaa
     4621 acaaaaaaca agcatactat atcgttaatg aatcaagccc tgaaaacatt tcaaacagtg
     4681 aagatattgt tttattgaca cctgtttcaa atgaaaaagg tgaatttttg acggatgttt
     4741 ggttataccc taaatcagag aaatcagagc cgaaagaaga aattaaaaaa gttgtgtcaa
     4801 ctggtgtaaa gaagaacttt tttgaaaatt gttgggaatt ttttgctcaa ttattttttg
     4861 ataattagga tgaaatgatg aaaataataa gaatattagt ttttaatcta tcctttttag
     4921 ccgcaacaat attgtgttgc ggtttaattt tttctcaatt atcaatttat ccaagatatc
     4981 aacgattgtt cattcatttt ctaaacattc aatgtgcttc catgccatta gaggtgatta
     5041 aaaagctctt gatcaatcca atttttatgg ttagtttggt tcttcttaca ggattggtaa
     5101 cgtgggtatt aggaaaaaaa gaatgaaaag cttacgatcg tttaaaaaaa tattagatgg
     5161 agggcattat ggaaaataaa attcattgca ctacgggaag agtaattact ttagtgttgt
     5221 ttttgttcct tcttcttttt gcgaggggaa ctgcagtcca ggccttagaa aaagttgatc
     5281 cgagtcaaat caaagaaaat aataatctgg tgctttatca tgatagtgat tcattgagaa
     5341 atatcgaagt aactgggatc aatggattta agatactaga tgagtattca actaaaaaac
     5401 aaaaagtact tgcttggcca aaagtcaaag atttgaaaag tacgtcaaaa atcgttgccc
     5461 attatcaaaa agtaggtact tatctaggaa aagaaattga ttgtgacgtg actttttcta
     5521 attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt aatggcaaaa
     5581 agtattataa ttatttgctc tttgataaaa atatttatcg aggatttttc atgcagtcaa
     5641 tcaatgtcat gaatgcaaaa ctagaattta agtatgcagg aacaaaaaca aaagtagctt
     5701 tgagtgaacc acagccagaa attcaatcaa aaatgagatc gatgagagca gctggtattg
     5761 atttaaacga tacatttcta tccatttaca gtttaaatgg acagtatcag caacgtgtgt
     5821 cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg agagaatttg
     5881 ttgggtatga aaaaatgggt agcttacctt attttgtcac aacagataca gcatgtgcag
     5941 aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg ggaggacgtg
     6001 cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct gatacttatc
     6061 acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg attggcgcaa
     6121 atacgaatag tatgatgttt agctttgata ctgcattgct atggacacca caaccatcga
     6181 agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac aatattgaca
     6241 aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag tttgatgcat
     6301 taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc tttgacagca
     6361 aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg tatgttgaag
     6421 cgaatggtac ttggaaaaaa gctgatacag gaggaaagtt aactgtatca ggtaatacag
     6481 tcacttatac tgcttctgac agctttttag ctaacgctgc aaattacggt agggattata
     6541 ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca aatgtggcta
     6601 ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataca gtagaaaact
     6661 atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt gatggtcgaa
     6721 atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa gtacaatatc
     6781 tagtcacaca aaaatggcat acaaaaggtg tagacattgc ctcagatcat tacaagcaat
     6841 ttagtattca agatccagtg gaagatcgtt tgacgtataa agaaggttct gcgcgagtga
     6901 tagatatgag tacaggaaaa gatattactt cagaaggtac actaacctat gatagcaatt
     6961 caagaacgct gaagtgggaa gcttccgctg atttcttaag taaaaatctt ttagatggac
     7021 gagaaattca gctgatattt acagctaaaa ctcctttgaa tgaagaattg gatatcgaca
     7081 accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta acgattgggg
     7141 taaaacctaa cttacctcag atcattgtgc caaaaactgg atcgaatcat ttagtgctaa
     7201 ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta gttttaaaac
     7261 tcaaataaat ataaaaacaa ggctgagaat cagccttgtt ttcttttatt tgaagattgg
     7321 attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa ctacaaaatt
     7381 atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat gggcagggct
     7441 atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt catcaatata
     7501 cagcccagtt tgacttatgt tttctccctt agcaccgtag cagcctaagc tttccaataa
     7561 atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta attctttagc
     7621 tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa caccattttg
     7681 ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac gtaattcgtc
     7741 taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt tattaatgga
     7801 tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca agaaataatt
     7861 ttgatcagtc atgtttttcc aaggagtagc aaagctatcc ccttcgtata ctgtttttga
     7921 ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca cattaaattt
     7981 acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg ttactgaatt
     8041 tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag ttaaagtgac
     8101 actgtcaccg tgagtgacat tgactatctg ttctttcaaa atattaactc gtccttcgtt
     8161 tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat tgaatgtgat
     8221 tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt tgtagctaaa
     8281 attatcttta gcgccttcgc tatatttaaa tgcttttgcc ttagcagtgt aagtagaacc
     8341 agaagttact tttgcttgat ctgtagcaat cacttgacct gtttgattgt caatatggtt
     8401 aatagtaacc gtatattctt ttggtgtttc aggtttgtgt tgcaaccctt taaatgcagc
     8461 atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat cgtctagtac
     8521 ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg taaaatctcc
     8581 tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat tcacagtagg
     8641 aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg cactatccac
     8701 ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta aagcggtttg
     8761 gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa cgctcgtata
     8821 aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc cataccaacc
     8881 gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt tccaacttgc
     8941 agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga tcggtttatt
     9001 atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat ttttagtcaa
     9061 ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt tattgtacaa
     9121 acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt cataatttcc
     9181 ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa aatgttcatt
     9241 attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa cagatccagc
     9301 aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat taaataattt
     9361 cattttttca actcctaaat ttaattaaaa atgtttacat gattattata tatcggctaa
     9421 ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt cttattttat
     9481 ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa tttcatagca
     9541 tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact tactgtagtt
     9601 aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc aaacagcaac
     9661 cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt ttctttatta
     9721 tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag aaaggtggat
     9781 ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt tcgttatttg
     9841 atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa ctatggtttg
     9901 tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat aagaaatcgg
     9961 aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa gaagtaggtg
    10021 gtcttattca taatttaaaa attatagatg atactacctg ggaatattct gaaagtgtat
    10081 ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa ggtttagaaa
    10141 gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct gattcattat
    10201 ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt ggatctagca
    10261 aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcagagtta aataaataca
    10321 aattacaaaa gactagtgag tgattgctag tctttttatt tatcctgaag gaggaatcac
    10381 agtggcaaaa gttaaaacac cattcgaaaa agccgcgtct aaaatcttag aacgccgtgg
    10441 tgtttctgaa atacagtgga aaaaagaagt tcttgaaaaa gcacaatttg acctattacg
    10501 aggaaaagat aaggacttag aagaatatat ctatcaacgt gaacgtgaaa aacttgtatt
    10561 cgatgaaatt attaaacatt aaaaggagcg aataatctta tgtttgaatc aattacaatg
    10621 aattttttaa ctcaaccgaa agtagtagct gcagttgata tttttagagc attacaaact
    10681 gccgcaacta gtttgaaaaa taatctgatg atttttgcgg ctgtagctgc cgttgcctcc
    10741 attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa cgggaagaca
    10801 cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc tattgtagtt
    10861 tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaaaca agtaggagga
    10921 gtaaaaagta cgtggctact ttttactcct ttttttatga aaggagaata ttcatgtttt
    10981 ttacgatttc aggaaatgca aaattggata aagagttaat aagaaaagca acgaagaaag
    11041 tgggaaagcg tacttccttt ttaccgaaaa caattaaata ttctgtcatt gaaacggaag
    11101 aacagctaca tgtgttagga caagtgttag aacaagagaa tcaatcttct gttaaattga
    11161 ctctcacgtt acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa tacgtttttg
    11221 gcccactgga ttatcaaatt tcagaaggtt tttatcctac attgatagat ttaatccgcc
    11281 ttgatgtgtt taaagatacg gatgaaacta tgagtgtgga agagtcttat cgaaacgaaa
    11341 aactgctaca agttttagaa gaagagttag agagcgacca aagaaaagtg aatcaagtga
    11401 gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc aatctttcca aagaaggagt
    11461 caaaagaaaa agagacagtc tatgttgacg aacaagctga tattccgact cagaaatcga
    11521 tatttgagga agagaacgat gtgatgggtc aattggaggt agatcccttt gaagaaacaa
    11581 tggaggatga agagacggac gaagtagaag ttgatccagt tgaagctaat gaagagtcaa
    11641 catggactga aggggatttt caggaatata ttgaaccact tatcagtgaa gaagtagaaa
    11701 aaaacgaaac agagatacaa aaggcaactg tatttgatta tattttggat caatatccgc
    11761 cagagcatga atggatgaaa gaaaaaaaca aattattttt aaatgagctt tattcgaaat
    11821 atcaattacc ccaaacctat caagcatttt tagagagtaa agaagaatta atctcacagg
    11881 gtaaaggcac acttttagaa cagttagagt ctgtccaatc aacaaattgg catgagcttg
    11941 cacaagaaga tttattagcc acctttgaag aacgtcaaca agaaacagat caagaaatta
    12001 atgagtatgc gacggaacag tcgtctaatt gggagaaagt aaaagaggaa ctaaataaag
    12061 aagaacagcg agtgattgaa gaagaagtac aaaaagtcaa agagcggtac gaaaagaaac
    12121 gcgacaatgc ttattccttg tgtgtagaaa agatcaacaa ttttactaag acgaatcaag
    12181 aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg tattaaagaa cgaaaagaag
    12241 agttctaccg cggtttacgt acagataagc taaagattag caaagaatta aatgaacaac
    12301 tgaataagct atatcaaaca acgactgaag ttcttatgga aaaacacgaa cagatccagc
    12361 aagaattaca agtacaaatt ccaaaatgga aaaaagagta tgaaattcaa caacaagaat
    12421 tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga gcgtgaacaa ttagaaataa
    12481 gaaaacggga acaggatctt gcggaacaaa aaatggcgat agaaaaacaa cggttagagc
    12541 aagaaaagga aaaagaagca gctgctcgta aggaacgtca agaacaaatg gatacattgc
    12601 gtcaagcaca gttgaattat ctagcacaac ctcaaccagt agctgctgtt caaacgccag
    12661 tgaacaacca agcaaattta actccttcac cccagcgttc ctttaacgga tggatggtgg
    12721 gatgtattgc aagtttaagc ttgcttctag gtggaggaac agtatttgct tttaatcata
    12781 tcaatcaatc ttcacaggct gattctgtag ccacaacggc gtcaatcaag gaagaagccc
    12841 aggaacaagc aagaaaagag tatgaacaac aattagaaga attattgaat cgtaccaatc
    12901 agtcaacaac agagtcaagc atggaaaata gcaaagagag tgatcctagt aaggctaaag
    12961 aaggatcaag cgattcaact gtacactcaa acacttccga aagttcagaa tcaaatgagg
    13021 tgaaataatg cgtattagag aaacaacgag acgaaaagac aaacgattca atttagaaaa
    13081 gaataggtat ctttctcatt tctatcgcta tactccttca ggagaagagc taccagaaaa
    13141 acatgacaaa cgtatttctc aaaagatttt ttatagtggc ttagcttatc ttcctttaaa
    13201 ctatgttgtt acaagaatac cagaagcatt tcatcgattt gagattattc caggaagttt
    13261 acaaggagtg aatatttttc agttacaaga aatgccgcag ttattccatt ttgattcaac
    13321 tggtttttgg aatcctttga ccccaacaac aagtttaggc acacgtttag ttgtttctac
    13381 gatttgtgtt gttccagctg tacttatggg attgaaaatg gacatgtggt ttcgtccgct
    13441 agcagatgga caaaaagggg ataatcgatt aatgacctta aaagaaatcc aacagattta
    13501 tccgcaaata cctgaaacaa aaactacttt tccaggttat ggaggtattc caatcacaca
    13561 ttacaaaaaa tattggtaca ttcaaacaga tactgttaat acgtgtattg ttggtacctc
    13621 tcgatcaggg aaaggtcaga ttgaagtgtt agccaccatt gacaatttat ctcgtgcaga
    13681 aaaacaaagt tcaatggtcg ttaatgatcc aaaaacagaa ttatatgttg gtagtaaaga
    13741 tgaactagaa gctagagggt atgatgtata tgcctttaat atcaccgatc cactgcaatc
    13801 aatgagtgat aatccattgg cactcatcgt gaaatactgg aaacgaggag acatagatac
    13861 tgcaacacag ttaaccaata ctttcacaaa tacgatttat tatgatgcca acgcttcaga
    13921 taacaaatac tttaacgaaa acgcgcagaa agctgtcaat gcgcttattt ttgcgttgtt
    13981 agaacaagct gatcaaacag gagattactc caaattgaca ccgaataatc tcgttgagct
    14041 tctttctgag attggcggtt ttaattatta tccagatcca gacaatgaat tcaaacagtt
    14101 gaatgcctta gatgaattta tgaatcagtt gccacctgga aacattgcaa aaaaacaata
    14161 tggggcaacg aaaattggat ctgataaagc gaagggaaat attttatcaa cggcaatcac
    14221 gggattgaat ccatttactc taacgaaaat tgcaaagatg acttctcaat caacgattga
    14281 ttataaatcg gttgggtttc caaaatactt agatttgaaa ttacatgaaa gtcttttaaa
    14341 tcaacgagtc gtagtagaat tttatcataa gggaaagaaa tttcatgaag aacgtgtcaa
    14401 agtaggctat aaaggattta gtgaaatcaa ttttgattgt catttagttg caggtgatca
    14461 agtttgttta aaatacgatc atcaaggaaa agaatatgtg gcttggtatg aattttctca
    14521 aagagtattg aaagacgaag agggtaatat cgtttatcgg aaaaaacgag gagaaaccca
    14581 tttgccagaa ttagaaaaag aagccgttct aacattagat gaggaaaaaa gtaacttatt
    14641 agtagagtct attcggatgc actataccga taaaccaatg gctattttta tgcttacacc
    14701 tgattatgat ccatctaacc atatcattgt tagtattttt cttagtcagt tatataaaga
    14761 attatctacg caatgtgtta agactaaagg agataaatgt caccgcagga ttcattttat
    14821 tttagatgaa tttgggaata tgccaccaat ggataatatg gaaggaatca tgacggtcac
    14881 agctggccga aacatgttgt gggatctgtt tattcaatct tatcagcaac tttatgcgaa
    14941 gtatggagaa caaaacgcag cgattatcaa agaaaattgt cagacacata tttatattat
    15001 gtcgacagat gataatacca tcgaagaatt ttctaaaaaa gtggggaata agacggttga
    15061 acaagagaac agtaccatca attcattagg tatgaatact catatcaatc gcaatgttga
    15121 ttcagatcgt atcttaactc ctgaaagaat ttctactttt ttagaaggag aaacgattgt
    15181 actcgctcct ttaaaacgac aagatttgaa aggtcataaa atacggccgt atcctatttt
    15241 taatcatggg gaaacgaatc ttccttatgc ttatcaatat attgaagatt tcaatccatc
    15301 gtgggatatc aatgatatag acatacattg tacacatgcg gacttggatc ttaaggcttt
    15361 acaaattgat tatgattatt tcctaccaga tgaatatgct agaaatctct atcatggact
    15421 gcatgatcaa ttagaagaaa aaaacgaaga aaatatgttt tataaaatcg ttcaatcttt
    15481 tgagaatgag acggctaaaa aattgttaat tcaattttac gaagaacaag cagcagatcg
    15541 acttcaagaa tatgttgaaa agttgatcaa aaataaagaa atttctgaaa tgattgggaa
    15601 acggttgtta ttggaatctg aaaaggtggt gaaagaatga tcattggagg aattttttct
    15661 ggcatagccg actggtggtc aggcttattc ggagaagggg atgcaaaccc caaaaccgta
    15721 acgaaagtct tacaagatta tgggaattat ctgcagtatc aagatgtatt tagttggatt
    15781 tggaacactt ttctgtgggg attgattaaa ggattatata atctcaatac tttactagaa
    15841 aaaaccattt atcaaagctt tgatttaaaa gacttattga acgccgctgg ggtaaatgca
    15901 ctatttcaaa gtttgattag taaggtattg gctctttttt gtgtcgtcac attaatttat
    15961 ttaggcttga aattttttat gagtaaacac ccaccaaaag tcaaaaatat cttggttaat
    16021 ttatttatgg ccatgatctt gatttttggt ggtagttctt taatcgatga agggttaaat
    16081 atcagtaaaa gcttttacgg agacgtcact gcagcaaata aagcagataa aacgggatca
    16141 cctgcttttc aaattattca aaataatgtg atcgatgtca gtacgttact agcaactgac
    16201 ccgaataaag tagaatccct accaacagat aaaagaaatg ctttaacagc gaaaaatttt
    16261 aaaacagcga atatcaatgg gattattact cctgaacaag ctaaaggtat ggcaggtaat
    16321 gataaaagtg ggttatcaga tatacaaaag gatcggttaa aggcattaca ataccgattg
    16381 gaactagatg ataatgggaa agaaattcct gttcaaatta gtgacgaagg cttagcaaaa
    16441 tatgtctata cttcagggta tcgtcgatac agtagtaaac caggtattat tttagctggt
    16501 gaatcttctt tggcagtagc ctatttattt atcctattta cgatcgttac ttgcattatt
    16561 gagctggtat ttaaaaaatt ctatttagtg attgccgcgt caacggactt tgagacaggt
    16621 caacggatga aaagtgcgat agaagatatt tctcaatcct tcttgttatt ggcgtttacg
    16681 attttagaat tacgaatcta taccttaatg ctttcaggta tcggtgattt gcatgcggca
    16741 ggaaagatca atggattctt gtatgtcgtt gctttgattg tactaaccat tgcgctattc
    16801 aaaggctctc aatcagttac gaaaattttc ggcgttgata caagcctgaa aaacggcagt
    16861 aattctcttc ttagcatgtt tgccttagga aatattgcga aaaatgtggg tagcggagct
    16921 aaaaaccttg caagtgctgg taagagtggt ttaagccatt taaatgatat tcgtaaaaat
    16981 ggatttggcc atcgaaaaga tgttacccct gataacgggg aagaacaatc agacgataaa
    17041 gctacaactg tacctaatag taagaaaaca gtttccgaaa tgttcgggaa agcgaaaaat
    17101 ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt aaaaggaaca
    17161 gctgaagatc ttacagaaaa agccaaagac accttagaag ataaattaga cggcacgaga
    17221 gctaaagaat tggctgaagc tgtgaagaat ccatccgaag cactagaaaa acaaaaagaa
    17281 aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca agtcaatgca
    17341 gcagtgaaag ctaatacaac aaccgaaaaa gaaaatcaag aaggtatagt gattccttca
    17401 gtcaaagatc accaagaagc gttgcactct aatgaaaatt cgattgaaga gaaaaattcc
    17461 atagaagaaa gaaattcaga agaaggaaaa aatggacagt taccaaaaac tgatccagaa
    17521 caatatcgct ataaacctaa cagtgacctt gaaaatgaga agcgaatttc agatttatca
    17581 aaagtaaatc aagaagacaa agcagaaaat gagggacagt taccaaaaac tgatccagaa
    17641 caatatcggt atagatctgt taactccgaa aatgaaaaac gcatttcaga tttaccaaaa
    17701 cgaaatcaag aagtacaaac ggatgaggat tccatcgttc tgccaaatga taatggaatc
    17761 gaagttggaa ctgaaaaatc aacgaaaatt ggtttaccta aggcacaaaa tttaccaatg
    17821 aaagatagtc gtttgaatca attcaaggat aatgatcaaa gttttgttca tcaatcacct
    17881 gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa atggaaatct gacaactgtt
    17941 tcttacaatt tatctgaaag tggtgagaca gggaatgcgt ccgttcaaaa tgttgtgaca
    18001 agagaagctg caccaaaacc tattcaaaac gttcatacag gttacaagcc agcaaatgtg
    18061 gcatttactg caccaacaaa aataaatgtg acaagagaaa caaaaaacag gaacaatgca
    18121 ttgaaagaac aactattgag ggatatcaaa caggatatca aaccataaaa ggcctacaag
    18181 ttaggccttt tttctatgta tgaaaaggag aaatcattta tggataaata tagtaacatg
    18241 aaaaaccctc caagatttag tggagaatgg aagtttttcg cttttttctt tttagatcta
    18301 ttaacgcttg gtggctgctt tttttgggcg aaaatggtca ataatcttgt ccacttacca
    18361 ctcttttttg cactcttgaa ttatggctta tggattgcga tagcgttagt gattgtttgg
    18421 cgaccctata ataacccagg tcaacgacaa atttttgcga ttatcgagtc tatttttttt
    18481 gcggacaaga accattatca tgcgttagat cctaaccgaa attatcatgg tacgtataag
    18541 gaggatgaat aaatgcgagt caaaggacaa aattcagttg aaaggaaatc cattttgcag
    18601 tttggaaaac cagaagagcg ctctatcttg gacttatttc catataaatc ctgtacagaa
    18661 gagggagtct taattactag agaagatcat tttcaacgtt attatcgtgt agtttctaca
    18721 gacgtggaag gattaaatga acaagagaaa ctagaacgaa tgaatcagtt gaccaccgtc
    18781 atgcgtacgt atgtgccaac aattaagtta gttagtttga ccacagagac ggatctaacg
    18841 gaacaaatcg tgcaaaagcg gcaattatta gataaaaatc gtatggaaca gtccttagga
    18901 agaaatctac aacgattaaa aaaatatgaa aagaaattag tcgaagaaat ccaggaattg
    18961 aaacgagcag aacaagaacg tcctgactta agcttctttt tcgtcattga aggaaagaca
    19021 atcaaggatt tacaaaccag aaaccgtcag ttaatgcgat ctagtggcat tttagggtta
    19081 aaaccactct acaaaaaaga attgatcaag attctgtatc gaatgaacaa tatgaatgat
    19141 gagtaggaat ggagaagaga gaatgaaata tccgttgata tggttagaaa aagatgttcc
    19201 gaaggaagag accactagaa aatttcctca cactttaagc ctgggtaagt tgggaaaagg
    19261 gcaatctttt aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagca
    19321 attaaaacag ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta
    19381 ggaaatcggg aaatagaaat tgtaaaatcc aaagggtatg atttagattt tttaaaacaa
    19441 gtgatgccac aaggtgggtt aaattttgat gaagaatatg tgacgtatgg taatggttat
    19501 tcacgatgtg tgacagttta tcactacaca aaaaatccta cgcttttttg gttggccaat
    19561 ctcatgaaca atcctcatac agtggccaca ctcgatgtta gcacagatga aaagcaaaaa
    19621 gtgattgatc aaatcaatcg tgccttagat gaattaaatg atcgatcaga taatgaacga
    19681 aaagcgactg accgaaataa aagcgcggaa gaatacctgg aactagcgga atatgctaga
    19741 aaattgactt ctaatgggga aatttctaag caagtcaaag tgagagtaca tgtttacgga
    19801 aacacgattc aagaagtaga tgaacgagcg caggaactga tcgaaaatct caaagggatg
    19861 gattatcgtg cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc
    19921 atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact accatcgtta
    19981 aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg agggacacaa
    20041 tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa aaatagtgta
    20101 cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt
    20161 aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga
    20221 aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct
    20281 gacggaatga tcaatccttt agagatattt gccacacgga cgtatgatga agcatccagt
    20341 ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaaca
    20401 cacttagaca aagtggtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg
    20461 aaagcagaat ttaagaccta cttaaatcgt ttttatgaag agtatggaac agtaccacgc
    20521 ggctttactt cctcaaattc gcgttcaaat actgatacgc aagtcacagg gaaagatcca
    20581 gaagcttatc cgacgttcaa agaattttta gactttttag gacaactgga attaccaggt
    20641 gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt
    20701 gagacgtatg gcatgatttt tgatggtcat agtacgattc gacaccttga ccaacaacag
    20761 ttggtctttt ttgacattga ctctattaca ggcttagatc gaggggtaca acaatgtcaa
    20821 atgtacatgg ctatgacgtt gatttggaat caggcattga ttcaaggaag aagacaacga
    20881 cgtttaatta aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt
    20941 gatgagtgcc ataatggtgc gacctgttct gcagtgaaaa gctgtgggtt gcaagtagtc
    21001 taaaaactat tgtaataact cttataacga gaagtcaaat gagtgggtaa cgccacaaag
    21061 ggcttccact gatactccga catgcaacaa gtagtttatg cagaccaacg ttgtgtgaag
    21121 ctcggtgaag tcggctgaag gataccgtaa ggtaaaagaa atagtgaata ccacgaaagt
    21181 tctctagagg taaagggtgt atccgataag tcgggtgtcc aaaaaaactt aatatggtga
    21241 gtatgtacaa acgtactgac gaactagcga atgtactgtc cgaaaggcat agctcctaga
    21301 aatgggagag acagtgagca aaaaatcact cattgtgcta acttgtggtt gagtaaaagt
    21361 cgttgttatg aaagaccatg ctgcgttaca ggcgtaggag aagttagggg gcaaaagcta
    21421 gcatctaagt taaggcatgt ggatagatat aagggaacag gggaagccat caacatggag
    21481 agtgcatact cgatgaagtg gtagtaagaa atatgtgaat gacaaataac ttactttagt
    21541 gatggtgaaa gtagagtcac gactgatgaa ggttctgtaa tggaactgga ggaacaggct
    21601 cgattcgtct gtaaaagtca tgaaacccgt tcaaccatgg gattctcgta tgactaaaaa
    21661 ggtcacgagc caattggagc gtgataaacc atgacaaaag aaacaagtcc aaaagatagg
    21721 aagttgcggc atgcagaata ttatggcatg acggaaatct tcgataagat atactccaaa
    21781 agtttgaagg gagataattt caagaatcta atgcagatta tcatttctga taacaacatt
    21841 gtcctagcat atcgtaatat caagagaaat cacggtagtg tgacaagagg agaagatgga
    21901 gtaacgatta aagatattga aaatctcaac cgtaatgaat tcgtagaaat tgttaggaag
    21961 cgattcgcac attataaccc tcggaaagta aggcgtgtag aaattcctaa atataatggt
    22021 aaattaagac cgttgggtat tccatcaata tgggatagaa tcgcgcagca atgcatctta
    22081 caagtattag aaccaatatg tgaagcaaaa ttcaataatc acagctatgg tttccgccca
    22141 aatagatcag cagaacacgc aatagcagat tgtatggccc gaatgaatca aagtcacatg
    22201 gaatatgtgg tagatgtaga tatcaaaggt ttcttcgacg aagtcaatca tactaaactc
    22261 atgagacaaa tctggtctat gggtatacga gacaaacagc ttctcttaat cattaggaaa
    22321 atgttaagag cacccgtagt acttccaaat gggaagttga tatacccaac aaaaggaaca
    22381 ccacaaggcg gaatacttag cccattactt gcgaacatca atctcaacga atttgactgg
    22441 tggatagcaa atcagtggga ggaaaaagat gccaaagagc taaaacatca ttttaggaaa
    22501 aatggagctt gggataaagg taatatctat cacaagctca gaaagagtac aactctaaaa
    22561 gaaatatacc tagtgagata cgcagatgat tttaaaattt tctgtagaaa ttataatgaa
    22621 gcgaaaaaag ttttctatgc aactaaaatg tggttacagg agcgtctaaa gttaccaata
    22681 tctgaagaaa agtcaaaaat cacaaatctt tcaaaagaag aaagtgaatt tttaggcttt
    22741 acgatgaaat tagtgaccaa aggtaacaaa aaggttgtta atacgcatat aggtacaaaa
    22801 gcacaaagca tgattaaagt gaaactaaaa aaacaaatca aacgaattca aaaaagtcct
    22861 aatagcaaag atacctttat ctatattgga gtctataaca gtatggttat tgggattcat
    22921 aattacttca aaattgcgac tcattgtagt gtagcaatgc gaaaaattgc aagagaactc
    22981 agaccaagtt ttgaaatacg actacgagaa caaggaatct caaaagaagg acaatacgga
    23041 aagtacgaag gatacaagcc ttacctacgg tcaggacaaa tacgatatat aagagggttt
    23101 ccaatattac cagtagggta tattcaacac agacatccga tgtgtaaaaa aagaaatatt
    23161 aataaataca ccgaagaagg tagggaagcc attcataaga agcaacaaac cgtagaagaa
    23221 tggaagatat cgtggttgtg tagcaatcca gtgcttagta agagagcaac gattgaattt
    23281 gcggacaatc gaatttcaaa attcatcgct caaaagggaa gatgtgcagt aacaggtgag
    23341 gagcttatct tatctgaaat gcattgtcat catattatcc cttatcatga aagcaaaagt
    23401 gatagctatg aaaacctagt gattgttact gaggaggttc atcgggtaat acacgctaca
    23461 caaagtgaaa cgattgagga acttctaaaa tatctaaaat taaatccaaa acaaaaagaa
    23521 aaactcaatg agttgagatt aaaagttggt aacgaagaaa tatcttaata aatatttgta
    23581 ggacgggaaa acagaatgtc ttttatagac gagggaacgc cgtatgcggt gaaagtcgca
    23641 cgtacggtgt ggactggggg aaaagacgga gataatatca aactcttacc tatcagtatc
    23701 gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg
    23761 taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa
    23821 tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat
    23881 ctttatgcgt atggatgaat ctattttacg tcatatagaa ggattagtgg gtaaatcaat
    23941 gacgaactca gaattagaat ccattcctca acaaaatcaa ggagacgctg tgatcaattt
    24001 tggctcaaaa gaaaccattc gtgttcattt tagtccgaac aaacgtcaat taaaattgtt
    24061 tgatggagga aaataatgga aaaccaaaat gaaagtttaa tcaagcagta tgttaagcgt
    24121 cgtgcaaagc ggcgcttatt tttgtggttg tttggtactt ctgcaggttt gattactatt
    24181 ttgatcactg tgttcgtgac cctatttcta atacttgcgg cgggctcgat tgataattcg
    24241 gattcagatt caagcagtgg tggcgaagct tttacaggag aatattcgga aggcttaccg
    24301 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgccgta
    24361 ggtgcagcag tgaaatataa attattacca agtgtgatcc tttcgcaata cggttacgaa
    24421 tcagcgtttg gtacatcggc gtccgcaaga aatgatctca actattttgg gattacttgg
    24481 tttgatggtt gtctgtttcc taaaggaact gcacgaggaa ttggtggcat tgaaggtggc
    24541 tggtacatga agtttcccaa ctcaaaagct gcattttcct attacggctt tatggtagcc
    24601 acacaatcca attttaatgc ttgtgtagga aataaaagcc ctggcgcaag cttattgatc
    24661 ttaggacgag gcggttatgc cgctgcagga attacggaag atagtccgta ttatacaggt
    24721 tgtatgtcaa ttatcacttc taataaatta accgaatatg atgaattcgc catcaaacat
    24781 tggggtgaag gtggcaataa caacggcacg attactggtg aatggacgaa tccattccct
    24841 ggcagcagtt tagataaaag ttccttcagc ggtggtcaat tatttggaac gaatccaggc
    24901 ggtgaattcc gtccgaacgg cttccatgat ggattagatt ttggttcagt tgatcatcca
    24961 ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt acgttggcaa tcctggtatt
    25021 tcaggtttgg gtgcgtgtgt gatcgtgatt aattacgatg gcttgaatat ggtgtatcaa
    25081 gaatttgcaa atagtacagg aaattctcga gtgaaagtag gcgatcaagt gaaagttggt
    25141 caagtaattg ggatacgaga tacagcacac ttgcatttag gatttactcg aatggattgg
    25201 cgtcaagcac aaggacatgc attcattgat gatggtacat ggatcgatcc gttaccattt
    25261 ttgaatagca gtaaaaaata gatcaggaga aaaaatatgg aaaataaaac gttaaaaatc
    25321 gtcttaacag ctgcaattag cgttagttta atcggcaata ttgcgttgtt ttggcagctg
    25381 aaagaggaac aagcagcaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgag
    25441 aagctcaaaa aaaattcctc tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc
    25501 aatgagaaaa accaagaaat cgatcagctg aagcaacaag atggcacaaa agcaaaagag
    25561 gaagtaactg aagcacaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
    25621 tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
    25681 gacaaattaa gtcctacaga cgatgatcac caacatgatg attacggaag ttactccttt
    25741 acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga acaaaatttt
    25801 gtcgtatttg ttgattatac aatcggcaat ccgcaattca aagatgtcaa accacaaaaa
    25861 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcatagac
    25921 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag aaagacaacg
    25981 tttcaaaaaa cgaaaaagtt atcttagatg gctgggaatc tttatgattc tcggcttttt
    26041 gattcttgct agtccgattt atacgaatca gaaggtagat ataccagatg aggtaagcac
    26101 gaacgccttt ttaaacacga aagagctgac aatggtttca aaagaaaaag gagcgaacca
    26161 gcaattagtt tttgaatttg cgatcgatga tgaagatcaa agcttgttaa aagaactggc
    26221 taatcttaat tatcgcgtag aagtaaagac agctaaaggg gattatcaag caatacgagc
    26281 caaagtgatt aaggtgtctg atgattattt tgcagtgaag ctaacgcatg taccagaaga
    26341 atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt gatccaaaag tggatatgca
    26401 agaaccacaa gatctgattt tttatattca cgaagataag gtcaaagatc acgtgaaaga
    26461 cgaagattat gaacaacatg cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa
    26521 agcagctcaa aaacaaatgg aatctttcca agcaaccatt gatttgaatg aagaattagt
    26581 gaagaagcta aaagatcaat tgccttatca agtcgcagat gatcaggaaa atactgaaaa
    26641 caagattaat agctaccagc aagaaattga aacatcgaaa acgcaactga aagatcaaga
    26701 agagatcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa agatttagaa
    26761 ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct gacaaagata ctttcgctga
    26821 gcagaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa tatgtcgaga
    26881 agtgaattta tctaacacaa caatatctga gttgaagaat ggtcgaaaga atttagatac
    26941 cttaaaattc agatcatttg aagctctata tgtagctgct aaaaaatatt ttacagctga
    27001 taacaaataa aaaacaggag tattttatgt gaaaaaagta tataaaaaat gcttatgaat
    27061 cagctatatc accttaacca tttgttaatg agatgggaga aaacattaac cacgattcta
    27121 acgcaaacat caagcacata aaacatttaa cactcctcct ttctggctga agatttcaac
    27181 caaacaggaa gacagcgtat caattatagc atacactttt tcttaaattc atttttcata
    27241 tgacaaaata tttccttcta atcgacaaag ttagaaggat tttttattgt ctaaatacac
    27301 aaataacctt aatttttaaa gagcataagg aaacgtgata tcactttctc acgaaaggtg
    27361 ttaggtaggc tgtgcctacc acgtttctaa gaaagataaa tgatatcaaa attttggatt
    27421 tgtgtgtaga tttgtgggta ggatgaaagc gaatgtgggc agtaaaataa gcgtttgtgt
    27481 gtagcaaaat caaaaaatgt gtagggatat gtgtagctac acatatccct acacataata
    27541 aatttggagg aaaatatgga cttattaatt agaaatattg aagaaaagta tattaataaa
    27601 attgacaaac gttgtgaaga acttacggtc aagactggca aaaaatggag tcgaaatcaa
    27661 tacctaaaag ttttgatcga aaatgatttt gatcacgctc ttctgaatta taaaaaagac
    27721 caatttgatc ggttgttaga aaaatttgtg gatattcaaa cttataatac gaaaacttta
    27781 gaagaatata ttgcaacaaa taatcaatta attggtttgt taattgaata ataaaaatag
    27841 tattgtgaat ttataaaagg aggaaatcaa ttttggcaaa agcaacaaga aaacatgtat
    27901 atctttacga tatgcatatc gaagtacttg atgaaataat ttctactcat gaagatatat
    27961 ctaattatag tgaagcagtc agacatttgt gtatgatgta tgatccaaaa atcgaagaaa
    28021 aaataaatcc aattcaaatt aaattgaatg caatgggaaa agaaatttct attttaaccg
    28081 aaatggtttc agagtttgcg gatcttcatt tgaaagatac aggaatacta attggtacaa
    28141 attcaactgt ttatcaagaa gcagaagaac gtgtggaagg aaaaattaaa tccaatcaaa
    28201 ccaaaaaata ttcttctaag ttaaagaaac cagtgaatga atctgttcat accagagtag
    28261 atccatttga tccaacgaag attctaaaaa gataaaagta gaaggagaac gtgatgaata
    28321 aatcgcctgc agttattctt acttgtcaat ttaccttgcc caataaaaaa tctttttcaa
    28381 catacattga ttatatgaca agagaaaaag cactggaaga aatggaacga cgtacgcccg
    28441 aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat attcctgaag
    28501 gagaaacttt ttcggaaagt aagacggata aagaattcag tgaaacgtcg aatgaagctc
    28561 aatcgttgat ggaacatgga atcaattttg acaagttaga ggaacaagat tttaccaaat
    28621 atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg acgcaaaggg
    28681 aacaaaaaga ataccaacgg ttgaaccaag caatgaaaaa atatcaaaca gagccacata
    28741 aaaaagcttc aggaaatctt cctggagttt tttctatggc ttcagacgaa gtgaaaggaa
    28801 gtgatctgaa ggagatcaaa caaatcttcc aaagaggaca acaaaaaggc tcaatcattt
    28861 atcaagatgt tatctctcac gacaattcgt atcttgaaaa aatgggcttg tacgatccca
    28921 aaacagatac cttagatgaa gaaggtttga aagcagctgg aagaaaaatg atggagacat
    28981 tattcgagaa agaacaattg aatgaaacag gttactggat ggcaacgatt caccgtaaca
    29041 ctaagcatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt aaaccgtata
    29101 ctgagattga aaatggtgtg agttatatcg taccgaaggg gaagagaaga caagcaacat
    29161 tagacgcaat gaaaaccaat tatgtacatg agttagaacg tttctcttat gaaaaggatc
    29221 gaatgatcgg tatggagaaa cgaaatttat tgacacgaaa aagtgatttg cgtaacacct
    29281 taacaaaagt agtaaaagaa ccagcacgtt acgatcgaca cgccatgaac ttattaaatg
    29341 aagtatataa aaccctacct gaaaaacgtt cggaatggaa ttatggagat gaaaatcgaa
    29401 ccaagctttc accatttaca aggggaaaac ttgatgagtt aaccaaatat gttttaacaa
    29461 aagaacctgg atatcaagaa tatatacaga tttcaaaagc attgaaagaa gaagccaaaa
    29521 gcctttatgg ggaaagcaaa agagaaacaa agaatgcaga aaaaaacgcc ctctttgatt
    29581 taaaaaagcg cacaggaaat gcgattttaa cagaactgaa gagacaggat agtcaaataa
    29641 aaccattaat agaaagtgtc aatcagatgc aatctattta caatgaacga tcctttcgaa
    29701 cactcaaaca gttgccaaca aatgaataca agtatagtta tcaagaattc gtaaagcgta
    29761 ggaaacaatt ccaacaaaga gttgtcacgc agcgaagctt gcgtcaatta aatcgaacaa
    29821 ttaatgcaca tgaagaaaaa taccacgcaa gtaaagcata tgaagagcaa cagcgaagaa
    29881 tccaaatgga acaagaaaga caataggagg aagagaaatg aaacaatatt tagatcaatg
    29941 gaaagtcata gaaggatcac taaaagaaga acgaatcgaa caacttcctg attgtttaga
    30001 aaaagagcat ttgtttcaga taagcgaaat gttgagaaac gaacagtttg atccaaatca
    30061 atttttagtt gttgagtacc cagctactgg tgtgtattgt tgtaatcatg tgaagggtga
    30121 aaaatatttt attattcaag aatacgaagg taagttagcg ccttattata ctacttggga
    30181 aataaatgaa gagggaatca ataatttccc atgtgaaagc attgaagagt ctatttctct
    30241 cacggaatgt taaataaata gagacgtaaa agagtcctga acgtgattgt ccaggactct
    30301 ttttcaatga attgttttcc gccaattcac tgagtggtgc gattcaattt tattatatca
    30361 aaagaaagtg agattccaaa gtgaagggag gaagaagaaa tgactcagac aacgattggt
    30421 gttgtggttg aaaaaagcag ggataatctc gtttttgtga cagaaattca aacaggtaga
    30481 gcttttgtag tcacagacaa agctgcaaaa gcgtatcaaa atggcaatat tttaacgtta
    30541 aatctgacta cgaagatgtt cgttgatgca gtagaaaatt atccatttgt ttagacaaac
    30601 gatattttac aaggaggaaa cggacatggc agaagaaaaa gaaccactag ataatagtcg
    30661 gttgggaaag tccaaaagaa aattagtacg gttgcaaaac gaattaaata atcaatttca
    30721 aaagctattt gatcaccaaa aattgacaaa tgggcaacca atgaatgaca aacgaaatgg
    30781 tcgttcatgg tttaaccagc aagaaaaaat agaaaataaa atacaaagtt tgagagaaga
    30841 gatcaagcaa caagaaaaac aggtagaaaa acttgaaaga caagaagagc taaaggaaat
    30901 gggctataac aagtacggcg gactggatat gacgatcgat aatattccaa gaatcaaaga
    30961 agaaatcgaa cgctttgaaa aaggtgaatc gactttttca gccgccacga tccgaaaata
    31021 tcaacggaag ttggaaacat tagaacagct gaaagaaaaa tcagaaaaag gaaaagaaaa
    31081 tatcctccct gaagtacaag cgatcattga cagcggacga gtgacgcaat ggaaaaaaaa
    31141 tccaaccatt tattttttga aaggctatcg aaaagttgct ttagaactga atgaaaacgg
    31201 gggatttgag gaatcaaaaa aataccaagc cacgtcggaa gaggaaaaag cagctgttcg
    31261 tgaattactt gaagaggctg caccaactga ttctctgaag gagaaagaaa aagatcattt
    31321 ctatcgagta gaattcaacg aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga
    31381 gatcgtaaca ttagaattga tcgatcaact taaaacgttg gataaccaat acgctgcaga
    31441 agcaggttat tataaattct attttgaaga agtgaagaac ggagaagtag tagatcagat
    31501 ccgaatggat ctaggggatg gcgtcaacag taatcacccc atctatttag agttagcaaa
    31561 attgtgcgaa caagtacctg agaaggaaca aaaacaatcg gtcaaaaatg aacctgtttc
    31621 gaaagaacaa atgcagtcga aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac
    31681 ggcagaagag atcaaacaag aagttaaatc cctttcgcaa caggcactgg atgcggtgaa
    31741 aaagcatacc cattcttctg aagacgtcaa agaacttttg aactttatgt ctcgttttcc
    31801 agaacgatcc tttagaaatc aattattgat tgaggaacaa tttccaggtg caacggcttg
    31861 tttaggtcgt gccgcgttga aaaaagaagg aatctatatc aaaaaagggg aacaagggaa
    31921 caagatcttt gtcagaaaaa cggtcaaagg attttatgag aaaggacgtg gctttgttcg
    31981 agaaacagaa gctacaccag aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat
    32041 caaaaaacct tactacacga ttgaaaaagt gtttgatatc acacaaacac agttaaaacc
    32101 tgaagactat ccgaaaatct ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg
    32161 acagtcagaa cttcaagctg ggattcaagc ggtagccaac aatattggaa tacaaattcg
    32221 agatatgaca gaaagtgaag tctatcaaag agaattagga caagcacgtg gggcgtatgt
    32281 tcgtaacgaa tttactggcg aagaagaaat cgtcatgaat actcgaaata ctcctacgca
    32341 gcatttagct acaagtattc atgaattagc ccatgcaaga atgcacaaat tttcagaatt
    32401 agataccgca actaaagaat tgcaggcaga aatgacttct tatattgtgt gcaaacattt
    32461 tggaatggac acttcggaga aagctattcc ttatattgca gcatggacga agaatggtca
    32521 gacgttggat gataaggaag cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt
    32581 cgcaaatgaa ttcattcaaa ccatttctaa tgaaatcaat caatacagag aaagagaacc
    32641 agagatccaa ccagcagaag tgaagcagga gcataaaaat actgtagaaa cggcagagaa
    32701 taagaatatt gtgcccgttg gttcactttg gatcgacaag aaagacggga aagtaatgga
    32761 agaagatacg ggtcgaacgt tacatttgaa ggatgcggcg tttattgaag atccgcagca
    32821 taatcaagtg attttaaaag gaaaaagtta tggaaaagag attcaaacgg cgttaccttc
    32881 agaagaattt gcgctaggaa atccattccg attttcactt gaaacagtgt cagaagtggc
    32941 caaacgaaaa aatgaaatct attatgaaca acaattgtca caaaaagaac gcgtataaaa
    33001 caagtaaaaa agactatctg aaaaacggat agtctttttt cttttgggag gtttgaaaat
    33061 gaccgaaaaa aaaatgagtc ttattgatcg atgtaaacag atcgatatag tggattttgc
    33121 tcgaaacaat ggaatggctg tagtcaataa aggacgagac tatcgcttag aagaccatga
    33181 ttcatttgtt tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg
    33241 cgacattatt gaattagcta aacttttttt catagacaaa gagatccaag actctaagca
    33301 acaatttaaa gcagccactg attttattct aaagaatgaa gacaaaacag aacgagtgga
    33361 aaatcttcat tttgaaacag aaaagtacaa agatcacccc gttgattatc aaccactcac
    33421 agaaaaagga aggaattatc tgaaggaaga acgaaaacta ccagagtggc tgattgatta
    33481 tgcagaaaaa gaagggttga ttgccgaact aaagccaaaa tatgaacggc aaaatttctt
    33541 agttcgagac gatcgtttag atcatgcggt cgccttttta tggaaagatc cacagacaaa
    33601 agagacagta ggggcgagtt atcaaggaac gttcattgat tatgaacgtt ttggtgagag
    33661 aggcacatat aagcatatag ataaaaattc gacagcgaat catggcttta acctaaagat
    33721 cggtgaccct aagcagttaa aattttttga gagtagtatt gatttactta gctatgctgc
    33781 actgaatcgt gatcagttaa acgatacgtg gttagtctct atggaagggt taaaacacca
    33841 tgtgatcagt cattattttg gtgaggcagt atctgaattg agaaagaaac aagcctttcc
    33901 tcaatccatc gagatttgtg tggataatga ccgtgcagga catattttct acgaaaaaga
    33961 acaattaatg ggcgcagtcg atccatttac gaatcagaaa gtacgttgtg aacgaggaat
    34021 cgctaatgat tggcaagtgc caaaagaata caaaatcatt tatgaagaag tcgcaaaaga
    34081 agaaaaggtc acaccagaag ctattatggc catacacaag acagaaaaca atctgcaatt
    34141 aacgaaccaa ctggtttccg ctcacaaagt aaatgcgttt tttggccagc agctttctgt
    34201 taacgattca atagaagcca ttaatttgaa agatatttgt agagaagtcg caaaagaatt
    34261 aaaagtttgt gaacgcgtgg atggtacgta cgattttgat cgtttttatc aagaaaaagg
    34321 ggatattaat gcgcagattc ttttctctta taaagcagaa caatactaca aaggctataa
    34381 aaatcatgaa catgaattta tacctgaagt aaaaaaggac tggaacgatc aattgaagca
    34441 tgagatccag caacaagaaa tcagaaagca aaaacgtgcc atgttgtttc aacaaggcag
    34501 acaacaagaa agggagtgaa atcgtcatga tgaaaaccgt tattttagca gaaaagccgt
    34561 cacaagcgaa agcttatgcc gatagctttt ctaaagctac ccgaaaagat gggtattttg
    34621 agatccaaga tcgattgttt acaggagaaa ctgtgatcac gtatggtttt ggtcatttag
    34681 tcgagttaga ttcgcccgat atgtacgatg aaaaatggaa acaatgggca ttagaacatt
    34741 tgcctatttt ccccaatcaa taccactatc atgttcctaa agataagaaa aagcagttta
    34801 gtgtcgtcaa acgacagctg cagtcagctg atacgattgt gatcgcgact gatagcgatc
    34861 gtgaagggga gctgattgcc tggtcaatca ttcaacaagc aggcgccaat caaggaaaaa
    34921 cctttaaaag actttggatc aactccttag aaaaagaagc catttatcag ggattccaac
    34981 agttacgaga cgcagaagag acctatccaa aatttgaaga agtacaagca cgtcagatcg
    35041 ccgattggtt aattgggatg aacggtagcc cactgtatag tttactcttg cagcaaaaag
    35101 gcataccagg aagtttttct ttaggtagag tccaaacccc aacactctat atgatttatc
    35161 aattgcagga aaagattaga aacttccaaa aagaaccata ttttgaagga aaagcccaag
    35221 taatcgctca aaatggcgca tttgatgcga agcttgatcc aaatgaaacc caagcgacac
    35281 aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca attaggaaaa cagccaggta
    35341 cgatccttca agtcgaaaca gagaaaaaaa gtgcggctag cccacgtctt ttttctttat
    35401 cgagccttca gtctaaaatg aatcaactca tgaaagctag tgccaaagat actttggaag
    35461 ccatgcaagg gttgtatgaa ggaaaatatc taagctatcc gcgaacggat accccttata
    35521 ttacggaagg ggaatatgcg tatctgttgg atcacttaga cgaatacaag cattttttaa
    35581 aagccgaagc gattccgact cctattcata caccgaatag tcggtatgtg aataataaga
    35641 aagtccagga acattacgcg atcattccta caaaaacggt tatgacagca gcggcttttg
    35701 aacaactaag tccactgcag caggctattt atgaacaagt gttaaaaaca acggtggcca
    35761 tgtttgcgga aaagtatacc tatgaagaaa caacgatctt gacgcaagtt cagcagcttc
    35821 agttaaaggc aatcgaaaag gtgccgctag atctaggttg gaagaagtta tttggaaaag
    35881 agagcgacgg aaaagagaag gaagaagagc cgctacttcc taaagtgaca aaaggagaaa
    35941 tggtgacggt ggatctacaa gttcttgaaa aagaaaccaa gccaccacag ccttataccg
    36001 aaggtacgtt gatcacggcg atgaaaacag ctgggaaaac cgttgatagt gaagaagcgc
    36061 aatccatatt gaaagaagtg gaaggaatcg gtacagaagc gacacgggcg aatattattg
    36121 aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa taagctcgtg gtgacgaata
    36181 aagggatttt gctttgccaa gccgtggaaa aagagccgct gcttacgagt gccgaaatga
    36241 ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg aaaaggaaca caaaccactt
    36301 ttctagccaa tatccaaaaa tttgtctctc acttattgga agtcgtacca gggcaaatcc
    36361 aatccactga ttttggatcg acgttacaag aagtgaaagc agccagtgaa aagcaagaag
    36421 ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca agaagtctta ctctatcaaa
    36481 aggtggcagc atgtacgtct gaagcctgtg atttcaaact atggacaacg attgccaaaa
    36541 agaaacttac agcgacacag ctgaaagaga tcattcaaaa tagccgaaca agccaaccag
    36601 tgaaagggtt aaaaggtcag aagggttcgt ttgaagcaac gattgtttta aaagaagact
    36661 tcactacagg ctttgaattt tctgaaaaga aaaaaacgaa cttcaagaaa agaacacgac
    36721 gcactacaaa ataatggagg gttaatctta tgcgtaatac aacacacaaa caacaagaaa
    36781 tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa aatgcgtgcc agcaaacgaa
    36841 gactgcagca attgaagaac cagcagcgga ttcaaaaaga aatcgaatta gatcgataaa
    36901 ttcctttctt gttaatataa aactaacatg ttatagttgt gttagtttta tattaacttt
    36961 aggggaagag taatgaaaga caaaatccaa tgtatttttg ataaacataa tggcaaccta
    37021 tccttgaagg atgctagaag tgaaggatta tcgcctatga cgttagatcg attagccgaa
    37081 aaggataaaa tagaaaaggt agcaccaggt ttctatacct taaaggatgc gttcgtggat
    37141 gaattgtatc tcgctcagag tatctattcc agagggattt tttctcatga gactgcgctc
    37201 gatctttttc aagtaggaac atatatccct aaaaagatca atatgatgtt tcctaaagga
    37261 tacaatgtat caaaggagaa attagatcag tatgccattc aaccgcatta cactaataaa
    37321 gaaaattttg acctgggagt cacagaaaca gaaagctttt acggcaataa aattttagtc
    37381 tatgatttag agcgaacgtt atgtgacatg tggaatccaa gatacaaagc aagcgtagag
    37441 gtaaaacaag aagcattaaa agaatatatg acaagtacca gtagaaatac aaataagcta
    37501 agaagataca tggatatttt aaaatcacca aaagaaatga ctttgtatat gttgccactt
    37561 tattgagagg agtaaagatg aatgacaccg acacaattta aagagaaaac aagaaatttc
    37621 gcagcagaga aacatttaaa cccacaaatt gtccaaagat attatatgat ggaaaaatta
    37681 ttagaatgta ttgttgaatc gccttatcat gatgattttg tattaaaagg gggatttctg
    37741 attggatcaa aatacgggtt agaaaaccgt tcaaccgttg atattgatac gacttatcga
    37801 aattcgaaac taacaaaaga aaagttagaa actgtttttt ctgaattaac agctaatcct
    37861 acaaaagatg gaattcgttt tgaattaaag ggattaacag agacgaggga agccgattat
    37921 tatccaggtt ttcaagcaaa gtttattgct tatcttgaaa atgcacgtgt accttttaaa
    37981 ttagatatta cttcaggtga ttctattatt ccagcagcat tgatgtatga acacaaatta
    38041 atgtttgaag ataaaacgat tcatattcca gcttatccta cggaacaaat tctagctgaa
    38101 aaattacatg ccacgtttag ttttggaaaa gataattcgc gcatgaaaga tttttatgac
    38161 ctgtatatga ttccgaaatt agaacgtatc gatgagaaaa atttgtataa gagtgttcaa
    38221 ggaacattca aggaaagaaa taatcaatcg gtgtttaagc atttgtatga tcaggaaatg
    38281 cctcaattgg tgaaagatga gggcttgaga gaaaagtgga aaagatacca acaagaaaac
    38341 tattttgcca aagacatttc tttcgaatct acactaactt ccgttgattc cctgatgcaa
    38401 tcaattgtag agcaagagaa ggcagaacga aaacaaaatt ttaagagatc acaacaaatg
    38461 aatcaagaaa tggagagata aagatgatca ataatgtaac tttagtaggg cgattaacta
    38521 aagatccaga tttacgttat accgcaagtg gcacagcagt ggcgactttt tccctagccg
    38581 ttaatcgtaa tttcacgaat caaaacggca atcgagaagc tgattttatc aattgcgtga
    38641 tttggcgcaa accagcagaa acgatggcga cattagctaa aaaaggcagc ttgatcggcg
    38701 ttgtcggacg aattcaaaca cggacttatg acaaccagca aggacaacgt gtgtatgtga
    38761 cagaagttgt ggccgataac ttccaactat tagaaagtaa agcagcgaca gaaagccgta
    38821 tgcacgctga tcaatcgtcc agcagtccaa gccctaccac ctttgaacaa cgggatacag
    38881 cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga caatcaatcg
    38941 atattagcga tgatgatcta ccgttctaag tagcattttc tattggttgc atttgctacg
    39001 gtttatgtta taatttagac aacaaaaaga attttcaatc tataaccaaa aaccactagc
    39061 tggcgggcta gtggctttct tttacgtttc tattataaga attaacgggt aggcttactg
    39121 tctttcttgg caaatttaca atcaagccat ttgcagatat agtgcgcggc tacttctgcc
    39181 atgattgcaa aaacaaaatc aaaaagaatt ttcaaaccta taacctcctc cccttgccag
    39241 attaggccgg ggtagcagac agcaagacta ttatagcata attgaaatat ccaaatgatt
    39301 ggatttttgc tataatttta gcgaggtgat tgtgatgaac aaatatgttg gtttgcttga
    39361 taaaatccga gtaataaaaa cgcaaccgtt attggttcgc tttactttac aaacgattca
    39421 tgaatcgatt aattgtgtag ttgcagatat tgaaattatc gataaattgc taattatgga
    39481 tgatggaaaa tacaatattg ccgtcacagg tcacttcaat aaacgaaatc agctagttat
    39541 tgctagcatg tatgttagaa atcccgatca ttttacgcga tcgatgggca tataaataaa
    39601 agaagagttg tagtcctaaa tgaggattgc agctcttttt gttttacata gaaacaggaa
    39661 aagacctttt taggaagaca caagtgcctt ctcaattgaa aaacctttct ctcactccaa
    39721 ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca cgttcactaa gatatggtgt
    39781 cttgcaaagc aagacaaaat agacaaaact tcctctcacc aaaaggttcg ttcctgttcg
    39841 ttatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc aaaaagcgac
    39901 aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc gttgatttat
    39961 tctggccaac aaatcaataa agaaagaact aacgaaagga agtaaaacct ttcctaagag
    40021 gaggcagaat caaacatgcg aaagaaattt gattattggg gtgttccctt cagcgaatta
    40081 ttcccaaact atcatgcacc acatactgtt gaatgcgatt gtggagaaag agcgaaatgt
    40141 atcaaaagct atcgtttgta tcaatgtcca acatgcggga aaaaatatac cttgtcttat
    40201 ggcgattaca ttttgattga tgagaaagga aagaaacgct gatgaaaaca caacacaaat
    40261 tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt tctgaaaaag
    40321 tagcagaagc tttacttatt ccgcggtact ggacgataga aaccccttta gtagaggatg
    40381 caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg cattatcaag
    40441 aaaacatgga aacttatgaa atcgaatatt ggttaaccaa attagagaac taaaaaggag
    40501 aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc
    40561 acaaaaaatg gaggatatga cgttcctata ccatgaaaca cgtgtaccca aaaaacatta
    40621 caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa tcaggtgtcg aaatgaatct
    40681 cattgcgacg tactatcgaa cactggaaga actgaaaaaa caaaatggaa aatggttttt
    40741 ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actatcaaac cttccgaata
    40801 tcaagcctta gaactgactt atgcaaaatt cgcacagatt aagaaagcta aagcaatggc
    40861 tagcgactgg ctagatactt ttgaaagtat cgaaaaaaat ggtgcatttt atacgattga
    40921 atcggaggag aaagataatg aataaggtgg tttatcagct aacaacagaa gatgtttcgc
    40981 aatttttaac aagcaaagaa cacttgaatg aagtaatcag acggattcaa aaagacttta
    41041 gttgtatgga agaaatgaag tactttattc aaagagtgga agaacagttg tatatggaaa
    41101 gcgaaacaag tcaaacgaag gtcacagtca gccataatca tttagacgtg tataaacgtc
    41161 gtcctgaaac ggtaaaaaaa ggaacagagc ttctacgact agaaacagaa aaatacatca
    41221 tgaatgtgta cctagaaagg agtgagctgg tcgaaaactt tgtcagctac acgatttgta
    41281 ggaaacacta ttactcagaa tcagaatatt attcaatttt cgagagcatg gaattaatgg
    41341 aagaagaaat ggaagagcta aacagcaaaa atgcgacggt agacgattat taccttgtat
    41401 ttactaggtt tttgacgtgt gggacggaat ctatcgtgtt aatgggaatg gattatgaac
    41461 tcgctgtacc cgaagcattt ttgagggaat atgtagaaga agtagataac ccaggcgacg
    41521 actggtacac gactttaact gattttttag acacctacac ttacgacgat acagaagaaa
    41581 tccaaaatga acttgaccgt aaacgaatgc catatcaacg atttgaaagg aaaaaaggta
    41641 aataaccatt tataccgaaa agtgaggatg tgtgaaataa gaacaaaaaa ccttgaaatt
    41701 aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc ccccgccgct taggtggtgc
    41761 gaaataagcg ggcaaagccc gataacctaa gagaaagaag gagatccata tgaataaaat
    41821 cattcctcta ttgttcgtac tgtttgttgg cgagatactt atgcgtcatg ctgcagctgt
    41881 acatttaaac ttcgtagtat tcttaggaat actggtattt tgtttgatct tttggcgatt
    41941 tgtattagcc cttgtgagtc tactattcaa gatgattgca gccatatttt tcatagggtt
    42001 atttcgatga aaggagagag tactaatgcc aattgcaata ggaaataaac ggttacccgt
    42061 gacattagat gaaaagagac aaaaagaatt gcagcaacta aagcagaagt acggaaaaag
    42121 tgaatctaag attatgtgta ttgcgttaga tctattgatc gcgcaagaaa aagcaggatt
    42181 tgatatacca gcactaaaaa agtgacgtca cttttcattt gaagaaacaa aagtgatagc
    42241 acttaattgt tcctactatt tagaatattt tctatgtcct ttttattcca ttttgtatca
    42301 ataactatga aatctttaaa gtatccagaa gagattattt ttagatcaat ttttttctta
    42361 cctgattcta aagattttgt tttcttaaac atattatgat ttcctttcga ctattttcat
    42421 ataaatgtta acatagttta tggattcatg gaatgttaat ctaatttaat agaaaaggat
    42481 aattaagtat atacgttttt tcttatgtat gttatactat gtatatacag aaagaggtgt
    42541 ctactatgga aatgttacct ataaaaaaat ggggaaatag taatggcctt agattgccaa
    42601 agtatattat ggaatatctt ggaattcata cagaagacaa agtgaagata atacaagaag
    42661 aagtaaacgg tcagaagcgg ctaattatcg aagcggctaa tatagaaaat gaattaacaa
    42721 ttgaacagtt atttgaaaat tacactgatg aaagaaatca tgtgacgatt caaaatttag
    42781 gtgaagcagt aggtaatgaa aaatggtgag aaaacctaga caaggcgata ttctattatt
    42841 gaatacagca cctcgatctg gacacgaaca gacaggtaaa agaccttata ttgttttaag
    42901 tcatgatatt attgcggatt atagtaacgt tgttacagtt gctccaattt ctagtacaac
    42961 tagaaactat ccgctatatg taaatataaa ctcagaatat gaaatgaaga caacaggaaa
    43021 agtattatta gatcaactaa cgacaattga ttatgaagcc agagagtgta tttttttgga
    43081 aaaagcgcat gatagtttag ttgaggagtt attgatgaaa gttagaacag tttttcaaaa
    43141 agtaagcaaa taaaatagaa acagcagcat gatcaactat tcgtgattga ccatgctgtc
    43201 tttttttatt ctatatacgg tttgtctcgt aacattgtag tcttttgcaa tcttagcgat
    43261 cgcaacacct ttttttagat cctcaacgat gtttttataa acaagacgac gttggggatc
    43321 ttttgcattg gcactgtata gcttaggacg tcctttataa acaccgttcg cttttgcaat
    43381 cttaatgcct tgtgcctgac gtcgtttaga ttcggtacgt tcttgttcag cgatcatggc
    43441 caaaatttga atgatcaaat ctttaatgaa tttatccagg agaggattgc caattgcttc
    43501 ggccatgatc ggtagacttg tgatgatcag ctgcacattt ttcttcttca agtaattgac
    43561 tgaagcgatg atctcgtcgt aatttcttcc aagtcgatca atagcttcca caataaaaat
    43621 atcttgatct cggacgaaat ccagtgcttc ttgaaaaaca agtcgatgag taatcgttgc
    43681 gccagattgt ttctcaatga aaattctctc tgcaccgaat tttttcattt cttcaatctg
    43741 ccgttgttca ttttgatcga tggatgagac acgtatgtat gcaactctca aaatttttac
    43801 ctactttctg caactattta aaaaacaccc taatcataca cgagaaaacc agatttttca
    43861 atgtcgcggt cgtgtctagt tagggtatac cttatataga caaaaactaa tgatggagga
    43921 aatagtatgt gtcacttttc tttttaaatg gcaatatttt ttgtaaataa atgtcaatca
    43981 agaaaattta aacgattatg acattaaaaa tatattttat gaattttcta gtatataact
    44041 tgatttatct gttaagattt attaagaaaa cgttttctta tttttgattt cgaggtgata
    44101 gacaatgaaa aataagttta ttttatcttt aggtatttta actattggaa ttagtgtaac
    44161 tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg tagtagataa
    44221 taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc caactaacga
    44281 cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa
    44341 aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc
    44401 tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt
    44461 acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag
    44521 aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga
    44581 tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt
    44641 acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag
    44701 aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt
    44761 ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac
    44821 tttctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac
    44881 aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg
    44941 agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt
    45001 acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg
    45061 ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac
    45121 acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt
    45181 caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga
    45241 caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga
    45301 gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg
    45361 gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt
    45421 ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat
    45481 ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt
    45541 gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt
    45601 ttttgtaagt gtctactgat aacttattct caaatgttgt aaggatttta ttcatttgtt
    45661 aatatta
//
LOCUS       DANKBF010000018        44912 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000018 DANKBF010000000
VERSION     DANKBF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            36..365
                     /locus_tag="PWY89_001025"
     CDS             36..365
                     /locus_tag="PWY89_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL2066077.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            549..1196
                     /locus_tag="PWY89_001026"
     CDS             549..1196
                     /locus_tag="PWY89_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL2066078.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            1211..2737
                     /locus_tag="PWY89_001027"
     CDS             1211..2737
                     /locus_tag="PWY89_001027"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2066079.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2786..5584)
                     /gene="ileS"
                     /locus_tag="PWY89_001028"
     CDS             complement(2786..5584)
                     /gene="ileS"
                     /locus_tag="PWY89_001028"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2066080.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5846..6553)
                     /locus_tag="PWY89_001029"
     CDS             complement(5846..6553)
                     /locus_tag="PWY89_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL2066081.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6574..7356)
                     /locus_tag="PWY89_001030"
     CDS             complement(6574..7356)
                     /locus_tag="PWY89_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2066082.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7405..7692)
                     /locus_tag="PWY89_001031"
     CDS             complement(7405..7692)
                     /locus_tag="PWY89_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2066083.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7707..8306)
                     /locus_tag="PWY89_001032"
     CDS             complement(7707..8306)
                     /locus_tag="PWY89_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL2066084.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(8320..8997)
                     /locus_tag="PWY89_001033"
     CDS             complement(8320..8997)
                     /locus_tag="PWY89_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2066085.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(9014..10255)
                     /gene="ftsZ"
                     /locus_tag="PWY89_001034"
     CDS             complement(9014..10255)
                     /gene="ftsZ"
                     /locus_tag="PWY89_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2066086.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(10278..11603)
                     /gene="ftsA"
                     /locus_tag="PWY89_001035"
     CDS             complement(10278..11603)
                     /gene="ftsA"
                     /locus_tag="PWY89_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2066087.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11759..12979)
                     /locus_tag="PWY89_001036"
     CDS             complement(11759..12979)
                     /locus_tag="PWY89_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2066088.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12994..14082)
                     /gene="murG"
                     /locus_tag="PWY89_001037"
     CDS             complement(12994..14082)
                     /gene="murG"
                     /locus_tag="PWY89_001037"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL2066089.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(14108..15469)
                     /locus_tag="PWY89_001038"
     CDS             complement(14108..15469)
                     /locus_tag="PWY89_001038"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2066090.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15483..16445)
                     /locus_tag="PWY89_001039"
     CDS             complement(15483..16445)
                     /locus_tag="PWY89_001039"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2066091.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16471..18663)
                     /locus_tag="PWY89_001040"
     CDS             complement(16471..18663)
                     /locus_tag="PWY89_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2066092.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18664..19065)
                     /gene="ftsL"
                     /locus_tag="PWY89_001041"
     CDS             complement(18664..19065)
                     /gene="ftsL"
                     /locus_tag="PWY89_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2066093.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(19070..20029)
                     /gene="rsmH"
                     /locus_tag="PWY89_001042"
     CDS             complement(19070..20029)
                     /gene="rsmH"
                     /locus_tag="PWY89_001042"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2066094.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(20050..20481)
                     /gene="mraZ"
                     /locus_tag="PWY89_001043"
     CDS             complement(20050..20481)
                     /gene="mraZ"
                     /locus_tag="PWY89_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2066095.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20651..21019)
                     /locus_tag="PWY89_001044"
     CDS             complement(20651..21019)
                     /locus_tag="PWY89_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL2066096.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            21190..22146
                     /locus_tag="PWY89_001045"
     CDS             21190..22146
                     /locus_tag="PWY89_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2066097.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            22219..22335
                     /locus_tag="PWY89_001046"
     CDS             22219..22335
                     /locus_tag="PWY89_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL2066098.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22390..24072)
                     /gene="recN"
                     /locus_tag="PWY89_001047"
     CDS             complement(22390..24072)
                     /gene="recN"
                     /locus_tag="PWY89_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2066099.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(24090..24539)
                     /locus_tag="PWY89_001048"
     CDS             complement(24090..24539)
                     /locus_tag="PWY89_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2066100.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24646..25464)
                     /locus_tag="PWY89_001049"
     CDS             complement(24646..25464)
                     /locus_tag="PWY89_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL2066101.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25473..26363)
                     /locus_tag="PWY89_001050"
     CDS             complement(25473..26363)
                     /locus_tag="PWY89_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2066102.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(26365..26589)
                     /locus_tag="PWY89_001051"
     CDS             complement(26365..26589)
                     /locus_tag="PWY89_001051"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2066103.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26594..27931)
                     /locus_tag="PWY89_001052"
     CDS             complement(26594..27931)
                     /locus_tag="PWY89_001052"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2066104.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27932..28786)
                     /locus_tag="PWY89_001053"
     CDS             complement(27932..28786)
                     /locus_tag="PWY89_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL2066105.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28883..29332)
                     /gene="nusB"
                     /locus_tag="PWY89_001054"
     CDS             complement(28883..29332)
                     /gene="nusB"
                     /locus_tag="PWY89_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2066106.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(29325..29750)
                     /locus_tag="PWY89_001055"
     CDS             complement(29325..29750)
                     /locus_tag="PWY89_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2066107.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29996..31060)
                     /locus_tag="PWY89_001056"
     CDS             complement(29996..31060)
                     /locus_tag="PWY89_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2066108.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(31232..31477)
                     /locus_tag="PWY89_001057"
     CDS             complement(31232..31477)
                     /locus_tag="PWY89_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2066109.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31616..31990)
                     /locus_tag="PWY89_001058"
     CDS             complement(31616..31990)
                     /locus_tag="PWY89_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL2066110.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(32073..32987)
                     /locus_tag="PWY89_001059"
     CDS             complement(32073..32987)
                     /locus_tag="PWY89_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL2066111.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(33008..33811)
                     /locus_tag="PWY89_001060"
     CDS             complement(33008..33811)
                     /locus_tag="PWY89_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2066112.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33844..34833)
                     /gene="manX"
                     /locus_tag="PWY89_001061"
     CDS             complement(33844..34833)
                     /gene="manX"
                     /locus_tag="PWY89_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2066113.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34936..35427)
                     /locus_tag="PWY89_001062"
     CDS             complement(34936..35427)
                     /locus_tag="PWY89_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2066114.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35618..38425)
                     /locus_tag="PWY89_001063"
     CDS             complement(35618..38425)
                     /locus_tag="PWY89_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2066115.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38859..39152)
                     /gene="rpmA"
                     /locus_tag="PWY89_001064"
     CDS             complement(38859..39152)
                     /gene="rpmA"
                     /locus_tag="PWY89_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2066116.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(39165..39509)
                     /locus_tag="PWY89_001065"
     CDS             complement(39165..39509)
                     /locus_tag="PWY89_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL2066117.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39536..39844)
                     /gene="rplU"
                     /locus_tag="PWY89_001066"
     CDS             complement(39536..39844)
                     /gene="rplU"
                     /locus_tag="PWY89_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2066118.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39864..39940)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            40126..40767
                     /locus_tag="PWY89_001067"
     CDS             40126..40767
                     /locus_tag="PWY89_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2066119.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40829..42460)
                     /locus_tag="PWY89_001068"
     CDS             complement(40829..42460)
                     /locus_tag="PWY89_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2066120.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(42583..42942)
                     /locus_tag="PWY89_001069"
     CDS             complement(42583..42942)
                     /locus_tag="PWY89_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL2066121.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(43101..43174)
                     /locus_tag="PWY89_001070"
     tRNA            complement(43101..43174)
                     /locus_tag="PWY89_001070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43138..43140),aa:Arg,seq:tct)
     gene            complement(43483..44166)
                     /locus_tag="PWY89_001071"
     CDS             complement(43483..44166)
                     /locus_tag="PWY89_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL2066122.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(44294..44812)
                     /locus_tag="PWY89_001072"
     CDS             complement(44294..44812)
                     /locus_tag="PWY89_001072"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2066123.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT        12748 a         9712 c         7580 g        14872 t
ORIGIN      
        1 ttttctcata aaggagtgat ttggtgataa aaaaaatgat ctattttctt ggttcactca
       61 ttaccgttct ctcaagtttt atttacttga aaaagaaaaa acaagcggaa aagtcagaag
      121 gactctcaaa gtatcaaggt acatggttct ttagtgatag ccagcaaaaa caacaattac
      181 agattacatc tgattacacg tttttgatca acggtcagtc tcttggcaca tctgtggtag
      241 aaatagatga atatcgttta gtcgtacgtg atcagtatgg tttttatctt atcttttctt
      301 atcccgaaca aactctttat gacgaatctg cagacacttc atataagttg ttatctgaaa
      361 aatagatccc tcccaatgta tatatatatc ctttataaac gaaagaatga gtcttctcta
      421 tcatctttcg ttttttgttg taaaaacgtc taaaaatcct ttgtttttta tttagaaaaa
      481 tagtcaatta acgttttttt tgttacactt actaatgatg gttttttacc atgaaaggat
      541 gatcgaatat gacaccaaat cgagaagatt atttgaaaat cattttagaa ctaggtggag
      601 atacaacgaa agtcaacaat aaacaaatcg tatctagtct agatgtctca gctgcatctg
      661 tcagtgaaat gatttctaaa cttgtgaaag aagagcttgt agaacattcc ccttatcaag
      721 gtgtacaatt gactgcaagc ggactgcaaa aagctagcag tctgattcgc aaacatcgct
      781 tatgggaagt ttttttagtt gaacatttag actatagctg gaatgaagtc catgatgatg
      841 ctgaagtatt ggaacatgtg acttcggaac atcttgcaga tcatttagaa aactatttga
      901 atcatcctga acattgtcct catggaggat cgattcctaa aaaagaggaa gttgtccatg
      961 aagaacgccg acaaacattg gagtcctatc ctgtcggtac aaaagtgcgt atcgcacgag
     1021 tgcttgatga aaaagaattg ttggattact tagtcactat tgacctgaat atcaacgaag
     1081 aatatgagat cgttgacatc gcagcttatg aaggaccgat cactatccaa aatgatagaa
     1141 agaaaatccc cgtcagcttt aaggcggcca gcacgatttt tgtcgataaa atataaagga
     1201 gtgtatccac atgtctaatg aaaaaaatgt attgtttacg atattcggcg gtacaggtga
     1261 cctagcccag cgtaaattgt acccgtcttt gttccgcctt tacagaaaag gaaatttagg
     1321 cgagcatttt gctgtaatcg gaaccgctcg ccgtccctgg agcgatgagc actaccgtga
     1381 agttgtcaaa gaaacaatcc gtgcattgaa tccttcagat gaagaagcaa ctacctttgc
     1441 aagccatttt tactatcaat cccatgatgt aaatgattca acgcattatc agacattaaa
     1501 agaattgtct gaccgattag atggacacta tcaattaaac ggtaaccgtg tctattattt
     1561 ggctatggca cctcaatttt ttggaacgat cgtttctcat ttgaaatcac aaaatattat
     1621 gacaaaaaat ggcttcaatc gtttgatcat agaaaaaccg ttcggttctg attaccagtc
     1681 tgcttttgaa ttgaaccagc aaatcagaga agtattccca gaacaagata ttttcaggat
     1741 tgatcattat ttaggaaaag aaatgatcca aaacatctca gctatccgtt ttgccaataa
     1801 tatttttgag gctcaatgga acaatcgcta tatcgacaat atccaaatta cttttggcga
     1861 aaacctcggt gtagaagatc gcgggggcta ctacgatcat agtggcgcac ttaaagacat
     1921 ggtgcaaaat catattttac aggttgtagc attacttgca atggaaccgc cagtagcatt
     1981 ttccgaaaaa gaaatccgta cagaaaagat caaagcattg aaagctatcc gcatttataa
     2041 cgaagaagaa gtgttagaaa acttcgttcg cgggcaatat gctcaaggtg aattggatgg
     2101 tgtacaattc aagggctatc gcgaagaaga caaagtggat gctcaatcag agactgaaac
     2161 attcgtcgca ggcaaattca ctatcgataa cttccgttgg tcaggtgttc ctttctatgt
     2221 acgtacaggc aaacgtttga ctgaaaaagg gactcggatc aacatcgtat tcaaacaagt
     2281 tcctgttaat gtattcaaaa catctgtcga tgaaccatgt gacgatacga cacttccacc
     2341 taatgttcta acgatctata ttcagccaac tgaaggattt tctttatcct tgaatgggaa
     2401 agaagtaggg caaggatttg aaacggaacc aatcaaattg gaatttagaa atagtgcaga
     2461 aatggttgaa aatagcccag aagcttacga aaaattacta ttagatgcgt tgaatggtga
     2521 tggaacgaac ttctctcatt gggaagaagt cgctcaatct tggcatatcg tggatgtcat
     2581 tcgaaaagct tgggaccaaa cacaaccgga ttttcccaac tacaaagctg gatcaatggg
     2641 acctaaagca gctttcgaac tacttgaaaa agacggattc gaatggatct ggcaaccaga
     2701 taactggtat cgtgaacgtg gaaaattaaa cgattgataa caaaaggggg tgtgacaaag
     2761 aacatgtcac accccctttt cccttttact tttcttcgaa tccttcagct acagcttcag
     2821 gatagaagtc ttcaacgatt tttgcacaac gaccgcatag atgaggaagt ttttcatcta
     2881 ctcctacatc tttacgaact tggcgacagc gatcgcaagt ttccccgtct gctttttcca
     2941 ctgtaatagc aacatcatca aataccatag cgttatctgg cacttgttca tctgctgcca
     3001 taacttcaaa gtagtctggt gaaacgatca ataattgtgc aatatctgca tccaaagctt
     3061 ttaacatttc acgaacttgt tgttttggat aaacagttag ttttgcttcc aaagatttac
     3121 caatcaattt gctgtttctt gcttcttcca atgcttttaa gacattatca cggaagtcca
     3181 tgaaagcagt ccaggtatcc atcaattctt cttggtttgc aaactcttca taaccaggaa
     3241 actcgcttaa ctgaacgtac tcctcttctt ctttcaggaa tgaccagatt tcttctgctg
     3301 tatgcggaat gataggagtc aataattttg tcaaagctac agctgtttga tagaaaacag
     3361 tttgcatgca acgacgttga taatcatctt ctgcttcaat ataaactaca tctttagcaa
     3421 aatccaggta gaatgacgat agatcaaccg tcaagaagtt cataactgta cggtaaatat
     3481 gcatgaagtt atatttgtcg tacccttctt ctcggatttc tttgattact tggttcaatc
     3541 gaacggtcat atatttgtct acagaacgta aatcattgta cgaaacagta tcttttttag
     3601 gatcgaaatc ttctgtattc gctaataaga aacgcatcgt atttctgatt ttacgatata
     3661 cttctgccac ttgacttaag atatccatag aaacacgaac atctgcttca taatctacac
     3721 tagataccca taagcgaagg atatctgcac caaattgttt gatcactttt tctggtgcaa
     3781 tgatgtttcc taaagattta ctcatcttac ggccttcacc atctaaagca aatccttgag
     3841 aaataactga tttgtatggt gctacaccat tgatagccac actagtcgtg atacttgagt
     3901 tgaaccagcc acgatattgg tctgatcctt ctaaatacat atctgctggg aatgataatt
     3961 ctggtcgctg acgtaaaaca gcttcatgag aagaaccaga atcaaaccag acatccatga
     4021 tatctttttc ttttgtaaat tcgccattag gagaacctgg gtgggtaaat ccttcaggaa
     4081 gaagttcttt cgcttctttt tcaaaccaga taattgatcc atgttcagca aataattttg
     4141 ccacatgttc gattgtttct ggtgtgatga ttgcttctcc attttcagca tagaaaatcg
     4201 gaagtggtac tccccatgca cgctgacggg aaattaccca atcgccgcgg tcacggatca
     4261 tattatacaa acgtgtttta ccccatggga taacccactc tactttttct acttcatcca
     4321 aaatattctg acggaattta tcgatagatg caaaccattg tggagttgcc cgatagatca
     4381 caggcttttt cgtccgccaa tcgtgaggat aactatgagt aaagaaatct agcttcagca
     4441 aagctccttt ttcttttaat agatcagtga tcataggatt tgctttgtca taaaagatac
     4501 cttcaaatcc aggtgcttct tctgtaaaca atccttttga gtctacaggt gacactacag
     4561 atagtttata tttgctacca gcgatatagt catcttcccc atgtcctgga gcagtatgga
     4621 ctaatccagt accagcgtct aaagtaacat gatcgcctaa aatcacaagt gattcacgtt
     4681 catagaatgg atgacttgca gtcatatatt ccatttcttg tccagcaaat tcttttagaa
     4741 ctgttacatt ttcccaaccg atttctgatt ggactgtttc tagcaaatct ttcgctacga
     4801 caaacttacg gccatcggct tccaccaata catacgtaaa ttttggattt acagcaatac
     4861 cttggtttgc tggcaatgtc catggtgttg tcgtccaaat gacaaaagct gtatcttcat
     4921 caagaatatc tttgccgtct ttgacattga aagctacaaa aatagatgga gattttacat
     4981 ctttatattc aatttcagct tccgctaaag aagactcact tgacggagac cagtaaattg
     5041 gtttcaatcc tttgtaaata tagccttttt cagccatttt accaaatacg cgtacttgtg
     5101 cagcttcata agaaggatct aatgtaacat aaggatgttc ccaatctcct gcaactccta
     5161 ggcgtttgaa atctgctcgt tgcttatcta cttgggaaag tgcatattca cgacattttt
     5221 ccaagtattc tgccatggac atttctttac gtttcacgcc tttattagcc aatacttgtt
     5281 caatcggcaa tccatgtgta tcccatcctg gaacatatgg tgcacgatag ccagacattg
     5341 attttgaacg aatgataatg tctttactga ttttattcaa tgaatgtcct aaatggatat
     5401 taccattggc atatggaggt ccatcatgaa gaacaaagct aggttttcct tcgttcaact
     5461 cttgtctttt ttggtagatt cgattttctt cccattcttt ttgccactcg ccttctcggt
     5521 ttggcaaatt accacgcata gggaattttg tttttcctaa ttgcaaggtt tctttcattt
     5581 tcattcaacc atacactcct ttatttgttt tccggacaca aaaaagcatg ttcagccgca
     5641 agggacgaac atgccgtggt accacccaaa tttagttgca tctatctagt gcaaactctc
     5701 tgtgaccaat aacgaggtca gccgctgctt cttactctaa tttcggaaaa gcaggtctca
     5761 agtagaggat ctttcaccat gcagtgtctt accgaacttc cactctcatc ggctcgcttt
     5821 aagaataaca aagtgctttt ctgttctact cgttttcatt ttcaccaggc acgatcgaga
     5881 tatcaatagc ctgtgtatca aatgccgtca taggtgcttc tatgactcct gtgtctgagt
     5941 ttacatcagt atcattttca ttgtcaagct gttttgctaa aacttctttg atcacttcat
     6001 ggctgtcagt cacgtagctg gagaatggtt gtaaaatttc atcccattcc ggacttttca
     6061 cttgttctaa ttgagactca agcatcaagc tgatacgttg atggaatact cgtgtttttt
     6121 tcttaagatc ttcagtttct gtcgcaagct gtcttgcttt ttctgttgca tctgtcaaga
     6181 tttttcttgc tttttcttct gcatcagttg ttaattgatg tgcacgtttt tcagcgttcg
     6241 caactaactc atctgctttg ttttgtgcag aagtaacgat cacttcggat tccttgcttg
     6301 cacttgtttt tactttatca gccgtatctt gagctacgat gattgattgg ttcaaagcat
     6361 cttttaattc attgaagtac tcaagttttt cttcagaatg tttgactgct ttttctaatt
     6421 ctcgatttct ttgagtcaat tcctcatagt cacgaacaac taaatctaag aaatcatcta
     6481 cttcgtcttg attatatccc cgcatttttg tcgggaatgt cttattttgg atatctaatg
     6541 gagttaaagc catgagtaaa ccaccttttt attttattta cgtaatacac cgaacagtac
     6601 cctgtacttt tcttttttcg tcttcccctc aagttcttgg atctggactc tgccataacc
     6661 tctgactgaa ataatatcta atagttcaag tacaaaatca ggtcttgtat tttctgtcca
     6721 gttcactttt actttaccag cttcgatcag ctgcttggac cgctgtctgg aaatattata
     6781 aatctcagag atcacactgt ctagtcgcat ggaactaact gttcctcttt cttgtgtcca
     6841 gctatctttc ggcataatca tttctgtgta tttgattggt tccaagcgta cagcaacctt
     6901 tccaatcttt gttacttgtg cagcaacaaa atgatccacc tcatttgcaa taaatacttg
     6961 ccagcgatca ccgtccgaga tgatatcgcc aaagtaagag cgtttgatcc ccgttcccac
     7021 taaagttcca aggattttcc catgacttag gctggcaaat ttgctaggat aatggatttc
     7081 atacaatgct gtttcaaaat cttcctctac aggttcgtaa taatcgggga aaatcaaaca
     7141 tctttttctt tctgcctgtt catatccgcc ataaaacatg aaacgcaaat cactgctttg
     7201 tcgaatcagt gtttctaaaa tataggattg tcttgggtca agaaattcgg tcagataggg
     7261 tgcatattgt ccttgaacct gttcaatcca atcgcctacc gaatcgataa atgtacgttc
     7321 atctgcccga aagtgctggt atacatttgc atccaaaatt ttcccctcct atctattctg
     7381 agaataaaga ttttgttgct tcaatcaata aactaaatgg acagttatcc atataacgat
     7441 ctgtgctaaa ccttgtacgg ctaagttcaa gatcaggatt gcccctaaaa ttgtgaaatc
     7501 caagggtcca atcctcaaag gcagacgatc gaacaaactg atatacggtt cgcagatctt
     7561 agctaataat cgtcctagcc ctgattgata cccgcctgga aaccaagaga gcaatgcata
     7621 taccatcaag ataatgctat agaaataggc tgcccgttga agtaaatgaa gtaatagtgc
     7681 aatagtataa atcgtagttc ctcctcttac agatcaaaca aattcgcatc tgcaaaagat
     7741 tgagcggttg aactgtcaat ctccatccca gtcggtgtac aaagaaaaat ctcatctcca
     7801 acacgtttga tatctccatc tagtgcataa actgttcctg ttagaaaatc aacgattctt
     7861 ctcgcttgtt tttcttctac taaatgaaaa ttcactaagg ttgcttctcc agcaatgatt
     7921 cgtttagcaa tcgccatagc ttcagaataa gctcttggtt ccattacagt gatttttcca
     7981 gcaagatttg ctttaggttg tgtagaggtt cttgaagctg ttctttcttt cgctacagtt
     8041 tttgtaggtg attgatgtag ttcaattact tttttctttt cagccggctg aatactttca
     8101 tgcgttacag gacgtgaagt cgccgttttt tgtttttcat gagtattcgc agcagataca
     8161 gaatgagttg attgattctg tgttgtattt tttttagttg gctgtacagc cttttttgtt
     8221 gtttcaggat aatcttggta ttcgtactct tcctcatcag ctaatccgaa aaaattagca
     8281 attgctcctc tgtttaaaag cggcattttt gttcctcctt tattcgtctt tgaatagcgc
     8341 tgttcctaca cggataaatg ttgcaccttc ttcaatcgca atttgaaagt cttgactcat
     8401 ccccatactt aattcactac atggtgcatg ggcataacct tttgcttgaa caagatcacg
     8461 caattctttc agcgtaccaa acactttatg gatttgctca tcagaagctt ctttaggcgc
     8521 catagtcatc aacccaacaa ccttgatttt tgtgcaggca gctaaagatt ccacaaacgg
     8581 taaaagctca tctggtgaaa ttccatgttt tgattcctca ccagaaacat ttacttcaat
     8641 aaaacaggat aattctttct ctaaccgctt ctggatttct tctgctaatc ttaaactgtc
     8701 caacgcatga aagtaatcaa tttcatttac aatcaatttt acctttctgc gctgtaaatt
     8761 accaattaaa tgccacttta tttcaggaaa atcaaataaa tcttgtttct tttctaataa
     8821 tttatctact cggttttctg cacaattcat aacgccagct ctaaccaatt cttcagcttg
     8881 agcggattca acagattttg ttacagctac taatgtaact tctttcggat cacgatgaac
     8941 aagagcacac gaatcctgaa tttcttgctt gattttctgc aagttgtcag caatcatgtg
     9001 ctcttcctct cctttatttt ttacgacgga aaaacggtgg cgtatttaat tcatcatcgc
     9061 catgagattt tgtttcttcg cggttgaatg tatcaaattc ttttttttcg atattgtcaa
     9121 aattggaatc atcaactcta ggacgaacat tttgttcacg tctgatatcc caatcgccaa
     9181 aactgttttc ttcttctgaa gaagttggtt tagcttgatc catatctaat acagtctttt
     9241 gagcaggtga ttgcatttgt gcttgtctag ctggtcggct agacttgcgt tccttttttg
     9301 attcatcgat acctgttgcg atgaccgtca cacggatttc atcgcccatt tcttcattga
     9361 tagatgtacc tagaatgata tttacatcgc cagttgctgc atttgctacg atatctgaag
     9421 catcttgtgc ttcgaacaat gtcatatcca aaccgcctgt gatgttaaga agtacttgtt
     9481 cagctccatc aatagatgtt tcaagcaatg gagaggagat tgcttttttc gttgcttcta
     9541 ctacgcgatc ttctccacta gccacaccga tccccatcaa tgctgtacct tggttttcca
     9601 tcactgtttt cacgtcagca aagtcaaggt taacgtaacc tggcgctgtg atcaaatcag
     9661 agataccttg tacaccttgt cgtaaaacat tatctgcttc acggaaagct tcaagcatcg
     9721 gtgtcttttt atctacaact tctaataaac ggttatttga gataattaat aatgtatcca
     9781 cattttcttt caaacgagcg ataccttctg cagcaaaacg gccacgtttc ggtccttcaa
     9841 aagtgaatgg acgtgtcacg acaccaacag tcaatgcgcc caattctttc gcgatacctg
     9901 caacgattgg tgcagcacct gtaccggttc ctccacccat tcctgcagtg atgaaaatca
     9961 tatctgcacc gtctaaagct tcgcgtaagg attgttcact ttcttctgcc gctttttgtc
    10021 caacttctgg ctgagaacca gcacctaaac cacgagtata tttggggcct aattggataa
    10081 ctgtttcagc ttttgaattt tttaatgctt gcacgtctgt gttggctgta atgaattcaa
    10141 cacctttaac gttttcttca atcattcggt ttacagcatt tccaccgcca ccaccgactc
    10201 cgatgacttt gattactgcg ccgtcattga tattattatc aattgagaat tccatatctt
    10261 attcctccca ggatgaatta atcaaaaata tttgtaaaga aacccttgat tttatttttt
    10321 acattttctc cggactcttt tggttccggc tcatcgtaga ttgtttcttc aggtgcttca
    10381 tagctctcat atgtgtttgt tgtttcttgt tcaactacca tttgacgagc tgctgaggtt
    10441 tcacccgtaa cagcaatttt tgccaattga tacacttcac tcaagtttgc tgaataatcc
    10501 acgatactta ttacatttgt aaataccggg tttctcagcc ccatgtgatt tggaacatac
    10561 aattttacat ttacgccgaa gatttcttgt gccaagtcca caacaccagg aagactagca
    10621 gctccgcctg tcaatacgac gccacctggc aactctaatg cttcgatttg gtccaatgca
    10681 tcttttgctt tgttgaaaat ctgttccatt cgagctgaaa taatttctga caagtagcgt
    10741 tcatcgactt tcactggttc tgactgacca atcacatcta ctggaaattc ttcatctggt
    10801 gatgtccgtt ctgggtatgc atccccgtag tttattttca acgcttctgc attgttaaat
    10861 gacgtattta atacaataga gatatcttta gtgacaaatt cgccaccttc ttggtcaaga
    10921 ttggtaaact tcaattgctt atcatgcata acagccgttg ttgtttggcc gccacccata
    10981 tcaatgacga tcgtaccaaa atctttttct ccgtcagaaa gaattgattc agtcagcgct
    11041 aaaggagtaa tcaccatttc gttgacgatt aatccggcat tttccacgca tttacgtatg
    11101 ttatggatga tcgttttagg tcctgtaaac aacagaccgt acatttctaa tcttacgccg
    11161 atcattccac gaggatcttt gattccctcg aacccatcga cagtaaaatc ttgtggcaat
    11221 attgacacga tttgacgttc aggaggtatt gaacgaacaa gggcagctga agccacattt
    11281 ctgacatctt catcggtgat ttcctttgaa tctccgttaa ctgcaatcat tccctggcaa
    11341 ttttctactt caagcatgtt tgctggcaaa ccgacactta cgcctttgat ctggatacct
    11401 gctttttctt ctgcttgtcg tactgctcgt tgtatcgctt ggaccgtttt atcgatatca
    11461 acgataatgc ctcggttaat cccttctgat tttgcgtttc ctactccaat aatattcatt
    11521 tggctgtcga tatattcagc cacgacaact ttaacagatg tcgttccaat atcaaggcct
    11581 acatacattc ctgtttttgc catgaatggg attccctcct acttactatt ccatatacaa
    11641 gcgaacaact agcgtccgtt ttaaaatata cttattaata gatatattca ctgcttccaa
    11701 ttctaccata aattactggt ttttagaaag tacattctta ctttttaaag ttgttttatc
    11761 aattttcatt taaacttgac ggagttgtac ttggattatc ttctatttga cttgtttcac
    11821 ttgaatcatt tgaatcagat gatccgtttg tgctagacga tccaggtgtt tgtgcttttt
    11881 gcgtttctgc ctcttcactt gattcgtttg tttgatccgc attttcttca ctttcagttg
    11941 tttcttccga ttggcccgat tcttccgttt cttgagtgct accgtaagga tgcgagaaga
    12001 taccaacttc catgtcgatc acaccttttt catccatatc tttggctact tgcgaataat
    12061 atttcatttg gttcgcaaga ttattgatat ttacaataac ttgattgccg tcattcatat
    12121 tcaattggat cagattttta ttgcttgctc ttggcgtgta cttgatttca gaaattgctt
    12181 tttgtaattc tgatgataac tgattgtatt gctttgttaa ttctttgatt tttgcagagt
    12241 ctttgaactc ttccaagata gggagatttt ttgtaggatt tgctactttt tcatctaata
    12301 ctgttccatt ttcaatcact gggtagtatt gattgttttt tgcgatcaaa gcaatctctt
    12361 tatattctgt tacatccaaa tcgaattcat tcatcccttt gaaatgtata tttgctgttt
    12421 cgattcttgg ctgtgctttt ttaaggcgat cgatataagt agatcgatga aaatactgtc
    12481 cccaaacatt ttctcctaat gacaattttg tatcagagat gatttcttgg ctgttgactg
    12541 tcttattgcc tgatactgca actgcttgta atttgctata aggagaaaca taatagatca
    12601 aaaaaatcag cggtataccc aatacagtga tgatcaaagc taaccgacga aatagtactt
    12661 tgtgccgttg actttttaaa ttgggcagcc ggttcaaaaa agaaccgtta tatggtccac
    12721 cgtttttctt ttgcttgatc tcttcaaaaa attcgatttt tttctcggac tggctttctt
    12781 cttgctgttc ctcttgaacc gttttttctt cttcagaatc tgaaaggggt gtatctaatg
    12841 ttatgtcttc tttttttggc tcttggtttt tcatctcttg ttctgccaga tattttcggt
    12901 tagccttttg ccaaggagtc aaattgtttt catcagaaga cggatgagac aatttttttg
    12961 gttctttttt cttactcact aagaccaccg cctttatttt ttgattattt gttttactaa
    13021 gtcataaaga cgttttgaag catcagggat tccttgttct ttagaagctc ggcacatttg
    13081 cttttgcaat tcttcatcac ccatgatttc gttgactgtc tgacttagtg attcacctgt
    13141 tagttcatta tctgcaatca tttttgctgc tcctgcgtga acaagactca ttgcattttt
    13201 agtctgatga tcattcgtaa catatgggct gggaaccaac actgccggta accccagagc
    13261 tgtgaattct gcgatcgaag ttgcaccagc acgccccacc agcagatcac tggaagccat
    13321 gacctctgcc atatttttga tataaggctg gacactaata ttttgtattt caggatttat
    13381 tttatttagg atctcttcat aatatctatc tcctgaagca tacaaaactt ggtaagaaga
    13441 aggaatatat tcacttaaaa aaactgatac tgcttgattg attttaagtg ctccttgact
    13501 accgccaaaa atcaagactg ttttcttttc tggatccaaa tcaaatgttt tcaaaatatc
    13561 tgatttttta gtatctgcta cttcttgtgc tcgaggatta cctacaagaa ctgctttgtt
    13621 ttcaggaaaa aaggaagccg catctttaaa tgaaagagcg atccgatcca catatcggct
    13681 taaaaattta ttggtaacac ctggtacact gttttgttca tggataactg ttggaatacc
    13741 cattttggct gcagcgtaca ctaccgctcc ggaaacatat cctccagtac cgataacgat
    13801 atctggttga aaatcactga ggattttttt tgcttgacgg atactcttaa gaaacaatcg
    13861 gaccgtcttg atgttatgca tggaaatttt tcgtttaaat ccttgaatct ccaaggtatg
    13921 gaaaggcatt cctgtatcag ggacgatttt gttttctaga cctcgttttg ctcccacata
    13981 catgaattca gcattcggtt cttgagtctt tacataattg acaaaggcaa gagctggata
    14041 aatatgtccc cctgttcctc cgcctgtaac caaaattttc attttttatc gctcgcttct
    14101 actttttcta ttttaatcgg ttcacagctt ccatgaatct gtttcctcta gtttcaaaat
    14161 ttgggtattg atcccagctg gcattcgctg gtgataataa aactacatca ccatctgtac
    14221 tgttttctaa tgctaaagga actgcttgtt ctacagaatc ggctgttact attgtctcga
    14281 tgcctgcttt ttttccagca tccatcaact tttcttttgt ttggccaaat acgaccattg
    14341 ctttgattcc ttttaatgaa ggaattaatt catcaaatgt atttcctctg tctaatccgc
    14401 cagccaataa cacgacctta gacgtttcaa atccacttag agccatttta gtcgctaaaa
    14461 tattggttgc ttttgaatca ttataaaatt ttctgccttg gatttctcct acatattgcg
    14521 tacgatgtgg tacgccatgg aaaaaatgca acgtttctcg tatagcttca tttgatatac
    14581 catacaattt tgctacagaa atagcagcta gtgcattttc tacattatgc ttacctggta
    14641 cgccaagttc agatatttcc attatttttt cttttttata gtatattgag taatcgtcag
    14701 catacacacc gtcttccaac acttcttttg tcgagaaagg cagtacgcgt gcttttgttc
    14761 tttgcgctaa ttcttgtagt tctgactgat tccagttaag gatcagataa tctttttctg
    14821 tcatgttttt ttgaaggttc catttagctt tgacatattc ttttcttgta ccatgataat
    14881 caatatgtgc ttcatagata ttggtaatca ccgctatatg agggcggaaa tctgtgattc
    14941 ccatgagttg gaagctagac aattccatca caattttatc ttctgatttt gcttcttgcg
    15001 ctacaccact tgctggatac ccaatattac ccgctaaacg tgcaattcct tgatctgctc
    15061 ctgcattcag tagtagtcct gtcatcgttg ttgtcgttgt ttttccgttc gttcctgtaa
    15121 tcccaataat tgggcattcc gctacttcgt aagctagctc aacttccgta attaccggaa
    15181 tacccatttc ttgggctttt gctactaaag gatgactgta gggaatccct ggatttttga
    15241 ccatcaatga aaattcttca tccaacaatt cgattggatg actacctgtt attactttta
    15301 tccctaaaga aagcagctct tgtgcttccg gattttcgtc aaaaggtttt ccatcattaa
    15361 cggtgacaag tgcgcccaat tcatgtaata atttggccgc acttactccg ctttttgcga
    15421 gtcctaagac aagcactttc ttattttcat atgttgatat ttttttcatt tcccgtcatc
    15481 tcctaccaaa cgatccataa tgtgatcaac gaagtcacaa tgctgatcaa ccaaaaaact
    15541 gtatcaatct tccactccga ccatccgcac atttcaaagt gatgatgaat tggagacatt
    15601 ttaaaaattc gttttcctgt aagcttaaag gaagtaactt gaagcatcac acttgctgtt
    15661 tccataacat agatcaaacc aatcaaaagc aatgtccatt cttggtttag cattatagaa
    15721 attgccgcta aaagtccgcc caacgctaat gaaccgacat ctcccataaa aatcttggca
    15781 ggttttctgt tgtagacgaa gaaacccagt aaacctccta aaacacttaa gcaaatgatt
    15841 aatacatcat actgatgctg atgccaagca attagaccgt aagctgcaaa agagatactt
    15901 cctaagccag caactaatcc atcgatgcca tctgtcagat ttactgcatt tgaaaatcca
    15961 acaagccaga agagagcgaa aataccataa atgattccta aaggtagatc tagtccgaag
    16021 aaattcaata cacctggata accttcagaa cggtaaacaa gataaaagat gattccccct
    16081 agaatctgtc caatcaattt ctgcttcgag ttcagtccca tgtttctttt cttgaatatt
    16141 tttataaaat catcaagaaa gccgattact ccgtataacg ctaatacaaa aagcaagatg
    16201 aaaagagtag gagttagctg tttttgccaa gctccaaccc aaattcctgt caaaatagag
    16261 ccgatcagaa acactaagcc gcccattgta ggagttccgg ctttgctatt gtgccacttt
    16321 ggtccctcct cacgaatcgc ctgaccttgc tttttcattt gaaaataacc aataaatagc
    16381 ggcattgcgg caattgtcat tgcacaactg ctgacaatgg gaatcaatgc ttgtgtccat
    16441 tccatgctct tttctctcct atttctcttc ttattcttct aaagtaaaac taagtttgtc
    16501 ttcagtgatt ttttcatatg gagcaagttc ttgtttgaca caataaccat ctccatcaaa
    16561 actaacttca ataccgagga ttttccccag cttcatgata tctgcttttg accatcctgt
    16621 tacgtcaggc atcagcttat caccaccagt atataggatc agtttttctc cagagatcaa
    16681 ctgatcaccg ttggcagtag attgtttttg tactttttta ccattaccga tcactacagg
    16741 ttgcagtcca cttttttgag catcagctgc tgcaacgtcc gcttcaagat tacgataatc
    16801 ggctacggat actttttctg tttttgtttc tgtatctgaa tcttcttcag tttctttaaa
    16861 atccattgcc cgtttcatca atggatttgc gattttagcc aatgccattc gatcatatgt
    16921 cttagggtgt ttcattgtta gatacaagac atagtctgga tcttcagaag gaaccatctc
    16981 cacaatcgaa tacaggtagg ctgtatctcc tgtttgatat ccccctgtat cagaagcgat
    17041 ttgggctgtt ccgttttttg ccgatacatt gtatccaggt acactatata caccataagc
    17101 actaccgtag ttttcgcttt cgacaacatc tctcatatat tcacggactt tttctgttgt
    17161 ttcttttgaa aaaggttgcc ctagaacttc tgtttgagtt gttcgctctt ctccagtttg
    17221 gggatccacc actttgctga tatatcgagg ttgaatcatt gtaccgttat tcgcaataga
    17281 agtgaaagct ttcatcattt ggaaattcgt cacacctacc gcttgtccat aagcactcat
    17341 tgctcggtcg acaatattta atgtaggtaa agcaccgttt acttcatcat ctaatccaga
    17401 atgagtactt tgtccaaatc ccagtttttg taaatagtta taccatcgtc cgcctaatcg
    17461 ctgttccagt atgaccatcc cgacgttact tgaccatgaa agagcttgac gcatggtcag
    17521 cacacctttt tcaccaaagt catggtcatt gatcgtagca tcttctactt ggatttttcc
    17581 agattggaag gtttcattct cgttgaattc tccttcttcg atagcagctg cagtagtgaa
    17641 aactttcatg gttgatcctg gttcataact atcttgtacc aagaaattac gccagatagc
    17701 atcttttcca gtcaatcctt ccattgtttc aggattgaaa gtaggtcgtt gtcccattgc
    17761 caagatttct ccggtttttg ctttcatcag gactgctgtc aattcttctg gctgatattc
    17821 ttcatttact tgatccatca gtgtttctaa ataactttgt agtctgctgt ctaaagtcgt
    17881 gtagatatct tgtccgtcga cagctttttc ttcttctgcc actgtacctg gcagaggatt
    17941 ttgaaaatta tctttttgat aaataatctt accatcttta ccgctcaaga tatcattgta
    18001 agcagactcc aaacctagtt taccaactaa gccattctca tctttgtctg gaactgcgta
    18061 tccaataaaa tgagaagaaa agacgccatt tggatacatc cgagcctgat ggtcgacaaa
    18121 ataaagacca gcgacacctt cttttttcat gtccgcttcg atgttttgct tggtttcttg
    18181 ggagatattt ttcccatagc taccaaattc aacttgatac agctcttttt ttgcgccgtc
    18241 ctctaaaata ttcagtgcat cttttttgtc tatagaaaga ttcttatgca ggatttctgc
    18301 tattttatca aaattctttt cttcaacata cagtttttta tccccagagg tatatgtttt
    18361 tgagagtatc gctttaattg aataagagga ggcatcttct gcgagcgcca caccgttacg
    18421 atcataaatc gttcctcttt ttgcttttac tacttcactt ccttggtaca actgttttgt
    18481 tttttcagct aatgaggtac cagcaacatg acctccaata acgatatacg agaaacggac
    18541 ggcaaataaa aagaacaatc caatactcgt agcaaataaa atgataccta cttttttgcg
    18601 attgttcatc ggatttagat ttttcttttc cataaaccgc cgaattttat ttttaagact
    18661 catttacttc actttcctta aattgtcgtc attaatagac aatccttttt cttctgcgat
    18721 ttgtttaatc cgttcagtac gagaaagttc gtttttttct tgttctaaac tcgttttttc
    18781 ttgcttttta tcattgattt ctgtttgtat tgacgtgatt tctttttcag ctccgctgat
    18841 atttgtgcgc agcataactg ttaaaacaca taaagcaaca atagcaatcg ccacgcttgc
    18901 cacagcaatt ttttccaaag cagaaatctg ctttaatcgt tttgccggtg attgtggaag
    18961 aatgatttct tgattcgaag aagaatcagg catttgttgt tccggctgat ccaccaattc
    19021 aggtaaatca tattcgtaat cttgcatttt ctttaattct gccatcattc tactcctctt
    19081 tcattcttat tttttctgca atacgtaact ttgcactgcg tgatcgattg ttttctgtta
    19141 gctcttcctc tgaaggaagg attggtttac gagtaatgac tttcagttct ggttggaatt
    19201 cgtcaggtat gacaggaagt cccggtggta gatcttgtac cgtactatat tctttaaaca
    19261 tacttttgac aattctgtct tccaatgaat ggaatgtgat tacgctgatt cttccattga
    19321 ctttcaataa ccggattgcc tgttctaaag aggcctcttc ggcaccgagt tcgtcattta
    19381 ctgcaatccg cacagcttga aaaattcttt ttgcaggatg cccgcctttt cttcttgcgg
    19441 gagctgggat gacttgttta atgatttcga caagctcacc agttgtttgg attggttttg
    19501 tttttcgtac tcgttcgatt tcacgagcaa tttgtttgga aaatttctcc tcgccatagc
    19561 ggaagaaaat ctttactaat tcatggtagg aatattcatt aattacgtga taagcggata
    19621 aaggagctga ctgatccatg cgcatatcca acggtgcatc ttgatgataa ctaaatccac
    19681 gttctgcttc atctagctga ggagaggaaa cccctagatc atacaaaatg ccgtctacct
    19741 tatctacatg ctcactcaag cgttcttcta attctctgaa gttcgctttg ataaatgtga
    19801 ccataccttg ttcgacataa gaagctaaac gcagcttggc gtgatcgatt gctttttgat
    19861 cttggtcaaa cgcatacaga tgccctttag aacttagttg gcttaataaa tattcactat
    19921 gtccggcacc gccgagtgta cagtctacat aaacgccatc cggtttgatg ttcaatccat
    19981 ctaccgtttc tttcaacatg actgtatagt gttgaaatgt ttcagtcatt agtcagcctc
    20041 ttcctattct tatagtccga aatcaatcat actttcagca atttcgtcaa agttctcttc
    20101 tgctgctgca gaaaaatctt gccatcgtgt ctcatcccaa atttcaattc ggttagatac
    20161 accgatgact acgcaacctt ttgtcaaagc tgcataattt cttaacgtgc tgggtatatt
    20221 gattcgtcct tgtttatcaa gttcgcattc agtggcagcc gaatagaaga atcgcacaaa
    20281 agtacgtgcg tcttttttag ccaacggcat ttcgttcagt ttagcttcca gattttccca
    20341 ttcgctcatc ggatagccga aaagacagcc atccaatccg cgtgtcagaa caaacttttc
    20401 gccaagttct tcacgaagtt ttgagggcac gattaaacgt ccttttgcat caatattatg
    20461 ttggaattca cccatcaaca ttgaaagccc cccattcctc cactaactaa aattagtcta
    20521 ccacaatccc ccactttcta ccaccttttc tgggaaatcc gggaacaact ttttttattc
    20581 ataaacattt ttttaaaaat caagctgcaa aaagcctttt aaaagacttt ttacagcttt
    20641 tctatttttt tcataggtaa gaaagtatgt tcacaatgat caagacagcg tacaagacga
    20701 ttgtcaacag aaaaaccaaa cgccaagtca tcttgaaata acgcggataa ataatttctt
    20761 cataataata tgcttggaaa acagcaatac aaattcccat tagcaaaaca gaaataaaga
    20821 aaaacggtaa gatagaaatt gtccctgtat ttctagacaa tgcatgcata ccgccccaca
    20881 aaaaaggaat cgccaaatca ggggctttta ttttaaaacg acgatttaac gaaaataatt
    20941 ttacaagaaa ttggcaacca aacaaaacga ttaccgggta aatataccaa aacaagataa
    21001 gtggtgtgaa tgtgcccatg aacgtaaact ccttttttat cgttgcttca agtatactgt
    21061 atatacttta cacaactgtt tcattttatt taaatttatg cagttatatt ctatatctta
    21121 caaacacatt ccatgtattc caattcccta tccgttataa taaaagaaac aggcaacagg
    21181 aggaaaatca tgatcaaata ttttacatta gataatgaga aactgcgtaa taccggaaag
    21241 gaaactgacc aaacgatctg gtattgtgta gaacgtccta atcaagaaga gattcatcag
    21301 ctgacacatc aatttgaaat tccgtcagat tacatcactt ccatattgga cgatgcagaa
    21361 aattcccgta cagaagaatt caatcaatca aagttgacaa agccagcgtt attattgtta
    21421 caatatcctt ttgctaaaac aagtcctagc ggttattttc aagtggatac atacccgctc
    21481 tctattattt tgacgcccaa aaagaagctt atcacaatta cgaatcatga accactgttt
    21541 ttaaagaaag tctttagcca gacctttcca aaaagtgata tttcgcctaa tctcaatatc
    21601 ttctttcaat tcatttggca ggtcacactc tcttacaaca gttatttagc tacgcttcaa
    21661 tctcaatgcg atactttgga agccaaactt caagtatcca ctgaaaacaa acaactttat
    21721 caaatcatgg atatccaaaa aagcctcgtt tatttcagag aagcgacttc tgctaattta
    21781 gatgcattga aagaactagc gcaaaataaa cttttccaag aaaatcatgc tttgaaaaat
    21841 catttacgtg atgtattggt ggaaacagag caggcaatga ctacttctcg cattcaatta
    21901 aaactagtag aacaaatgaa tcagactttt tcagcaatcg tatcgaataa tctgaataat
    21961 attatgaaaa ttttaacttc tttaacgatt attttaacta taccgacaat cgtcggcagt
    22021 atctatggaa tgaacattaa gttacctatt gctgaaaggg atgatgcctt tatatggcta
    22081 gttctattaa cattatttat ttgtatcgta actacctatt atttaaaaaa agggaagttt
    22141 ttataaaaaa gccctcttgt ggaaatggaa aaaagattgt acacttgaat caactaatta
    22201 atgacgaggt tgaaaacaat gcgtgaaaaa aaacaaacaa gtacttttgc taaagtaaca
    22261 aaatttgtca tttggacaat gctgatttta acgattggat ctgttgtatt gactgctgta
    22321 atgagtgtca tgtaaaaata aaatatagct atcagctgct gaaaaggacc tgaaccactg
    22381 cgaggtggct caggtccttt ttgcgagttt caatagttct tttgcatgtt cgagtgtgag
    22441 ttttgtaatc tcacttccag ccagcatacg tgcaatttct tcttctcttt cttttgtttt
    22501 caactctgaa acagaagttt gcgtacgtcc atcaatcact gctttcacaa tataatattg
    22561 atagtcggcg actgcagcaa cttgaggcag atgagtgatg cacaaaactt gagagtattt
    22621 cgatatttga gaaattttat ctgcgatggc ctgtgcaact cttccgctca cacctgtatc
    22681 cacttcatca aaaataatac ttgtcacacc ttgctcagaa gaaaaaatag ttttcaaagc
    22741 aagcaacatc cgagacagtt cgccaccaga agcgactttc accaaaggct tcagtggctc
    22801 tcctggattg gtcgtaatgt aaaattcgat ttggtcaaat ccatcatgat tcaattgtct
    22861 attttcttct tttaagaaac gtacctcaaa ctcagtgttt tccatataca gacttttcaa
    22921 ttcatgcaga atggattttt ccaattcttt tgcgattttt ttacgcgctt catgaagatc
    22981 tgcggcttgt tgctgaagta gaagctcttt ttgctctagt tcctctttca ttttgtccaa
    23041 ttgaccttct gaaaaatcag aagaagctag ctcttcggtg atttcgtcat agtaagccaa
    23101 tattgactca atggattcgc cgtatttacg tttcaactgt cggattaatt ctaaacgttg
    23161 cgtaacttct tctaaacgac tctcatctaa ctccaataaa tcaatctgac gactcatatc
    23221 gccgattgca tcttgcagca aatagtaagc actttggata ttttcataaa ttgcttcata
    23281 ctccacatct agatgggcga tcccttgcag ttctgaaacg gccaagccaa caccatctac
    23341 actgttttgt tcattattcg tcagtgctcc gtaacctgca gctaatccat cagcaatttt
    23401 ttgataattg ctaagttttt ctctttcttc ttttaatttc tcttcttctc cgacttgcaa
    23461 atcagcagca gctatttctt cttgctggaa atggagcata tctattctct gaacatagga
    23521 tttttcattt tgctgcactt ttcgtaccct tttttccagc tcacgataag agatatattc
    23581 ttgctgataa ctctttttct tctgttggaa agctacatct ccaaaacgat ccaataaagc
    23641 aagatgtgat tcaggctgga gcagttcctg atgttcgttt tgtccttgga tatctactaa
    23701 ataactgccg attcttctca gattagctaa tgtgataata tgaccattca ctcgacaaac
    23761 attttttcca gttaaggaca tatcccgtcg aacaatcaga ttgtcctcat ctgtttcgat
    23821 ccctaattca accatcagtt cagagaatcc ttcttgtttt ggtagttcaa acaacccttc
    23881 aagcacacat ttttctgctc cttgacgtat ataatcgctt gaacctcttc ctcctgctaa
    23941 cagacctaaa gcatcgataa taatggattt acccgcacca gtctctccgg tcagcgcggt
    24001 cattccttcg tgaaaagaaa gccgcaattc cggaataatc gcaaagttag taatactgat
    24061 ttcttgtagc atcacattcg ctcctcaact taaaggtaat gcaggaagtc tttcttcaat
    24121 aaaactgcct ggtcttcagt tcgtgtaatc atcaaaacat tatcgtcatc attgataatc
    24181 gaaaataatt ctttcttgta acgtttatca atcaaatttg ctgctgccga agcatttccc
    24241 ggcaatgttt tcaaaataac aaatttttcc atttggtcaa ctgccacgaa agcgtctttt
    24301 aatagttttt ccaatttagt atttacatct tcgtttgttt cagctggtat gctataacgg
    24361 tagccgccat ctgaagccgg taccttgatc agtttcagtt cctttatatc tctggaaatc
    24421 gtagcttgag taacagatat tcctttgctt ttcaggattt caacaaattc ttcctgcttt
    24481 cggatatctt gctcactcaa caatcgagta atcaaacgat gccgatcttt ttttctcatt
    24541 acgtcttcgc tccttagcat attcttctta atcaatgata gcttatttcc ttggtataat
    24601 aaagaagaat ccgcatactt ctcacttgat tttcacagtt acaatttatt tatggtcaaa
    24661 ctgttcgtgt gcatttgaaa ccacttctgc catattaata gctgaattga ttgttccgct
    24721 ttcgggtact ttcctcaagt gagccaagaa ctcgatattt ccttctcccc ccgtgatcgg
    24781 agaataactt aattccagca catcatatcc accttgtgcc gcaaatgaca aaatgtcttc
    24841 taaaactttt tgatgggtct gagggtcacg aacaattccg tttttcccta cagcttctct
    24901 gcctgcttcg aattggggtt tgattaaagc aatcacttct ccctcttctt ttaaaatagc
    24961 atgtaatgga ggcagcatca gttttagtga aataaaagaa acatcgataa cagcaacttc
    25021 tggaattcct aaggtaaagt cttcgggttt tgcataacga aaattcactc tttccattac
    25081 gacgactcta ggatcttgtc ttattttcca agctagctga ttgtagccaa cgtctaaagc
    25141 ataactcatt tttgcgccat tttgcaaagc tgcatcagta aatccgccag tggatgcacc
    25201 aatatctaac aatgtttttc cttcaacaga tagatcaaat gctttgagtg ctttttctaa
    25261 ttttaatccc cctcgagaaa cataaggaag ttttttacct ttgactctta attcactact
    25321 cactggaatt ttttctcccg gtttatcaaa ccgctcgttt ttttcgttgt aaatcaaacc
    25381 agccatcact gaacgtttgg cttgttctct tgtttcaaat aatccttgtt tgaccgctaa
    25441 tacatctacg cgttcttttt ccattctttc cctcatttct ccacatcaaa ttgcttgatc
    25501 atctggcgca tcagcccgcc atcaaaagaa aaaacgtcct cttctaattt gtctatgatc
    25561 ttgttggcgg ataacagctc aatctccaac gcctcacgtg tcttttctag gcctaataaa
    25621 cgcgggtaag tatttttttc catacgttca tctttaccag ctgttttccc taatgttttc
    25681 gttgtaccaa tcacatcgag tagatcatct cttatctgaa aagctaaacc taaatgttcg
    25741 gctaatcgtt gaagtagcaa taagatttct tcaggttgct tcgctaatat gccaccagcc
    25801 agcaaggcat aacgaatcaa atgccctgtc tttctttcat gaataaatgc taattcttct
    25861 aaagaaagct ttttcgattc gccctcgata tctgcagctt gcccagcaac cataccttgt
    25921 gatcccgcac aaacagctaa ttgctgaagc agcagaagct taggagaatt acctaagtgt
    25981 gccatactga ttagctggaa tgctcctgta agcaaaccat ctccggccaa tatagctaat
    26041 gcttcgccga atactttatg attggtaggt tttcctctac gaagatcatc attgtccatc
    26101 gcaggcaaat catcgtggat caaggaatac gtatgcacca tttctaaagc agctgctact
    26161 tgatagacgg gaacatcaat aggctcatca aaggaagcaa cggccgttaa aagtaataag
    26221 ggacgtattc ttttcccgcc tgctttcact gaataactca tcgcatcata gagtcctcta
    26281 tccgttgtat gctcatctaa aaaagacaag atttcttttt ctacagcagg tagatgaata
    26341 gctgaaaagt cagttaatct catctcagct ttcctcgttt ccgtcaaaat caacttcttc
    26401 atttgattct gtcatcattt tcgtcaaggt ctcttctgct ttagctaatg tatcttggca
    26461 atgtttgctt aattccattc ctcgtttaaa cgaatctaga gctgattcta atggaacgtc
    26521 tccttgttct aactgcatga caattttttc taattcttgc agtgactctt caaaagttgg
    26581 attttccata tttttattct cctttgtcta tctcttgtac ttgtccttta acgattccat
    26641 cttgataatg gatcgtcagc aaatcccctt gatgaatctc ggtgacactt tttactactt
    26701 gatcatttaa cgtcgtatag ctgtagcctc ttcccatgat tttcaatgga ctcaacaaat
    26761 caagagattg aacagcttga gcaaaacgct gctgctgttg tttcatatat tgttggattt
    26821 tctcttctaa gcgtttttct agataagcag tttcttggcg agctgcttta actcgataaa
    26881 gcggagccac ttgttccaat tggtggctta atgcgatcgt ttgtttttct ttcgcatgat
    26941 aaatttgctg tgtagcttga tataatcgct gtttcaactg gtctaatttg atggtctgac
    27001 cttcatacaa tctttcaggc tgccgaaata cataggaacg tcgagcacgc tcgaaccgtt
    27061 cttgtttgcg ctggatctgg cgtacaaacg cctgctgcaa tctagcttgc cgttcttgaa
    27121 tgcgcatcaa ctcttctgac aaaacaggaa cagctaattc tgcagctgct gttggagtgg
    27181 cagcccgaac atctgctact aaatcagcga tcgtggtatc tgtttcatgt cctacagatg
    27241 aaatcaccgg cgtattagct tgataaatcg cccgagcaac ccgctcttca ttaaacggcc
    27301 ataaatcctc aatcgaacca cccccacgac cgatgatcat tgtatcgaaa ttgcccatct
    27361 cttctactct ctggatattc ctgacaacat tgtccgctgc ttggtttcct tgtacaactg
    27421 ttgggaacaa aaccaattgg acaattggat atcttctttt caccgtcgtg atgatatcgc
    27481 ggataaccgc tccgcttgga ctggtaagga ccgcgattcg ctttgggaaa cgtggcagca
    27541 tttttttagg cccttgaaaa aggccttcct tcatcagctt ttcttttcgt tcttccaatt
    27601 cttgatataa agcacctatt ccatcaggct ccatgtgctc tacatagatt tgataagaac
    27661 ctcccgcttc atataatgaa atacgcccaa cgacaaggac tttcattcct tcttttggct
    27721 ggaatcgtaa tttttggaat gctcctttga acataatagc agatattttt gcatggtcat
    27781 cttttaagct gaaatattga tgggcattgg cacgtaaacg gaaattcgat atttcgcctg
    27841 ttaaatatac gcgttctaaa taaggatcgg catcaaattt tcttttcaaa tacttcgtta
    27901 atgtagtgac agttaaatat tcttgtgcca tctattcacc tgctccaaaa gcttcggcag
    27961 attttacggt ttgatacatc agcatcgtga tagtcattgg ccctacaccc tttggcacag
    28021 gagtgatcca gcctgcaata ggctccactt catcaaattt cacatcaccg atcagcttac
    28081 cgttagcatc acgattcatc cctacatcta tcactacagc acctggtttt acgaattctt
    28141 ttgtgacaaa atgtccacgt cctattgcta cgactaaaat atctgcttct tgtgccagtt
    28201 cagccagatt ttcggtttta gaatgagcaa cagtaacagt tgcatctgcc atcaagagta
    28261 gctgagccat cggtttaccg acgatattgc ttcgtccgat aactactgct cgtttacccg
    28321 ccggatcgat agaataagct tcaaacattt tcatgatgcc gtatggagta caaggaatct
    28381 tgtccggttt acccgcaagg agtctgccta aattcatcgg atgaaaaccg tctacgtctt
    28441 tttcagggcg gatagctaac aggatctttt cttcatctat atgctttggc aatggtaatt
    28501 ggaccaatat accatgaaaa ctaggatctt gattgtattt ttcaatttct gcaagtagtt
    28561 cgtcttcaga aatcgtctca ggataacgct ctaccaatga atggattccg atttcttttg
    28621 ctacacgttc tttatttctt acatagattt ggctggctgg attttcccct accaataaaa
    28681 cgactaatcc gggatggata ccattatctt tcaatttttg gattttttca gcaatctctg
    28741 cctgcatttt ttcagctaat tcttttccgt taatcaattc tgccatctaa ccactcccag
    28801 atattttttg tgttttcatt ctaccataga attttcccga tttataacag gtttatgatg
    28861 attcatagaa aaaagctagg acttacgccc cagcttctaa ttctttcaat acattggata
    28921 aaacaccgtt gacgaattta cgtgaacggt catcactaaa tgtttttgcc aactcgattg
    28981 cttcatttaa agcaacagcc gctggtacat ctgtcacata agtcatttca aaaatcgcga
    29041 tacgcaagat caccaaatcc atttttgaga tccgattgat gtgccaattg ttttttaaat
    29101 gtttttcaat cactttatcc aattcatctt tttttgcaca gacaccgcca actagcgtat
    29161 caagataaac aggaacaaac tctgattcat cttcgttgat catgtcgcgg tggtctaatt
    29221 cgattgcgtt gaaaatcgca tcttcttttg ttaggtctgc attgaagtct aatggaaata
    29281 aagcctgcaa tgccatttcc cgaatctcgt gtcttgataa ttctttattc attctcttcc
    29341 tcttcgtctt caaaaagttc ttcgattgtt ggttgttcta atttttcagg aacaacagct
    29401 actacatgga tattgacttc agataaatcg atatccgtca tgaaaaggac ttgctggctg
    29461 acccgttcct gcatttccat tgctacttta ggaactgaaa caccatattc tagataacag
    29521 taaagatcta ctttcaagcc atcttcctcg tttgctaagt aaacaccttt tccgtgagct
    29581 gcacgtccta ataattcagt gacattgctg gcaaaagttc cgcgcattcc gtatacgcct
    29641 tctactttag aagctgcaat cccgataatt acttcgatga cttcaggagc gatcacgatc
    29701 tctcctaaat cttggtttgc gtttaaaatc aaattttttt catctgccat ttatttattc
    29761 ctccctttgc ctaagcacga gatacatccg aattctctat gatgcggcat agtgatcacg
    29821 tggtaagaaa actgccgccg atcagctgca aactgctatg aattggagaa aacacccaac
    29881 tttattatct cttacagctt aaaagttctt ctatagtgta tcattaaatc gagtgatttt
    29941 ctataacaaa gtctgtgaac actgattttt acgcttactt cttctattga tgaaactata
    30001 agatgatcaa ttcttttggt gaacgagtca acacacgatt tccatcttct gtgatcaaca
    30061 agtcatcctc aatacgtact ccaccgattc ctggtaagta gatccctggt tcatcagtga
    30121 tcacatttcc tggtacaaat tgcttttctg cacgagaaga aacgtttggt ccttcgtgga
    30181 tctctaaacc gatcccatgt cctgtagaat gtccaaatgc ttccccgtaa ccatgttttg
    30241 aaatataatc tctagcaact gcatctaatt ggacaccagt cattcctggt tttgcagcat
    30301 caataactgc taattgagca tctaagacta tttgataaat ttcttttaat ttttccccag
    30361 gatcaccaat agcgaatgta cgtgtcatat cggaaacgta cccttcgtaa tagcaaccaa
    30421 agtccaatgt gatcaagtca ccttgttcga tgattttttt gcttgctaca ccatgcggca
    30481 tagccgaacg caatccgcta gcaacgatcg tatcaaaaga aacacctgaa gcgccaagag
    30541 aacgcatata aaagtctagt tgatttgcga cttcaatctc agtcatgccc ggttggatca
    30601 ttttcaggat gtgatcatat gccatatccg caatatgaca tgctttttcg ataatagcga
    30661 tttcttcttc atctttcact tctctgagtt cttcgatcat ctgtgaaaca ggtactaatt
    30721 ccgcatcgat gatttcttct aaaaccgaat attctaaaaa tgagactgtt gtttcctcaa
    30781 aagctaatga gtgcagttct tctttgcgta ctaagtttgc tacctcatca aaaataggtc
    30841 cgacattttt aataatttca aacccttgag cctgcgctgc cgcctgctcg gtataacgaa
    30901 aatcagtaat aaaaaatgct ttttccaaag taataacagc taatcctgtc gtacctgtaa
    30961 agttcgttaa gtaacgaaga ttgtacggac tggtaaccaa aaatgaatcc aaattttctt
    31021 cttgcatttt tttgcgcaat ttttctacac gtagcatcat aatgaaactc cctctttctt
    31081 tgcacgcttt tttccattat agcaaacttt tcttcgtaca cgacagttaa ataaaaattt
    31141 ccggaaaact tctttttatt ttaaataaat aaagccaaaa aggagtcaca gtctcgctgc
    31201 aactcctttt agtttactat tttgtaaaaa cttatgatcg ttttccaaag aaaaatgaga
    31261 caactgctac caaaatcact gcgcctaaaa tagatggaat aagtgccata cctgcagctt
    31321 ttggtcccca ttcgccgaaa atcgcttgtc caatacttga accgactagt ccagcaatga
    31381 tattcgttac acagcccatc gaatttcctt tactggtgat cgcacctgca attgctccga
    31441 tcacggctcc aacaatcaat gtccataacc aactcataat ataacctcct tctttgctaa
    31501 tcaattttag catatgggat ttaaagggga caaataattg gttttgctat ttcatatttt
    31561 cttctgcaaa gtaaaaaaat aaagaaaacc tcctcttaga agttttcttt attttttact
    31621 tgcgtttaaa catactacga ataccacgtt tcactacatc aaagaaactc aatgattgta
    31681 ccattctatc ttcatctaca tattctctga tcgcacgaag cactttttta ggttctttgg
    31741 aagaaaatgt gtatgtacca ttttttttcg tttgaatggc atatcgagga atccattttc
    31801 cctttagcat gactgatgct agtacataat ccacttcttc ccatggaatt tggatatatt
    31861 tgcgtctatc tcgcgcatgg taaaactcaa aaccattatt acctatcatg atttgcccgt
    31921 aatctgtcag acccgtaaac gaagtagcat caattaccaa gtctactttt gtattgattg
    31981 attgaaccat tttctccctc cagatatttg tttatgaaca gtatactata taaaaaaagc
    32041 tcgggacaac gcccgagcta tatttttatg gattataaaa gtccgatgac gtgtccaaca
    32101 acaccgacta cgaataaacc aaggatgata acgattggag aaaccttttt cttaagcaac
    32161 cacatacata acaatgttaa tcctaatgca gctaaaccag gaatcaattg gtctaagttg
    32221 tcttgtaatg ttgttacctt ttcaggagaa agggctaagc cattattaac ttgttcaaat
    32281 gcactcttga ttccttcacc acctgaaggc aatttatccc attcgatgta agcaccttta
    32341 tctaatttta cagttgatac aactggtaag aatttgattg atacccatct ttgtacaagg
    32401 gcagctaata cgaacatccc taaaattgaa gcaccttttg ttacgtcttg taacaatccg
    32461 ccagaaaggt catcagtaat tttagaacca gctttgtagc caaattcttg tgtataccac
    32521 atgaatgccc aacgaataat gttccaacct aagaagaaga tgattggtcc aaggatattt
    32581 cctcccatag ctaatgaagc acctagtgca cctagcattg gacgtactgt aaaccagaac
    32641 actgggtcac caacaccagc taaaggaccc atcataccta ctttaacccc ttgaatcgct
    32701 acatcatcaa caggcgcgcc attcgcacgt tcttcttcca gagccaatgt aacccctaaa
    32761 attggagaag caatgtatgg gtgtgtatta aagaattcca agtgacgttt taatgctgca
    32821 gaacgttctt cttttgtttt gtataatttt ttgattgctg gaatcataga aaatgcccag
    32881 ccaccgtttt gcatacgttc gtagttccat gaaccttgaa tgaaggtaga gcgccacgca
    32941 acagctaaac gatctttttt tgataatttg attttttctt ctgccatttt attttcctcc
    33001 ttcaagatta atagtcattc aaaatgtcgc ctaaagggtc accagagttg cttccgcctc
    33061 cattatttga tgaaccaccc attttagaaa ggttcaagta aataagagct aatgcaacac
    33121 ctaaagcacc tagagcaatc aatgttaatt gagaaattgc agctacaacg aaaccaataa
    33181 cgaagaatgg ccatacttct tttgtagcca tcatgttgat taccaaagcg taacctacag
    33241 ctacgaccat tcccccaccg atagccatac catctgttaa ccatgctggc attgattcaa
    33301 ggaatgattg cacagtttgt gctgggataa atagcaacgc tgctgctggg accgcgatac
    33361 gaaccccttg taaacataca gcaatgatgt gccacatttc aaccttacgg atgtttcctt
    33421 cttcagcagc cgcatccatc aaatgaacga ttggaacagc gattgtacgt acgatcattg
    33481 ttaagaacaa ccctgcaaca gccaaaggaa cggcaatcgc gatcgcagaa ggaacacctt
    33541 taacaccttg tccacctaaa actaaaataa ttgctgatgc tactgatgct aatgcagcat
    33601 ctggtgctac agcggcaccg atatttgccc aacctaaagc gatcatctga agcgtaccgc
    33661 caagtacgat accagcttcg aggttaccgg taactaaacc gatcaatgta catgccacta
    33721 gtggttgatg aaattgaaat tcatcaagaa taccttccat accagctaga aaggcaacta
    33781 atacgactaa aattattgaa ataatagaca tgataagcct cctataaatt ttaatattat
    33841 ttattacgct tttgctaatt cagcttttgc ttttttcaaa atatcatcca tgttatcgcg
    33901 tgaatcatta ggtactttac gtacatcgaa tttcacacct ttttgttcaa gcttctcaaa
    33961 cgcttcaacg tcttcttgtc ccatagacaa tactttactt acaacgactt taccgaccga
    34021 atgcgccatt gacccaacat ttaattcttt gatgtctacg ccaccttcaa cagctgtcaa
    34081 tacgtcttca ggattttcaa ataaaagcaa tgctttggta tttccaaaac gagggtcttt
    34141 tgctacttcg atcattttgc taataggaat aacatttgct tttacacctg gaggagcagc
    34201 ttgttcgatt agtctcttac gcagatcatc ctttgatact gcatctgaaa caacgataat
    34261 acggtttggt tgcactgctt ttgtccaagc tgtcgcaact tgtccatgta acagacgtga
    34321 gtcaacacgt gctaatacat atttgatttt tccgtctccg accacagttc cttctggaag
    34381 tgctccttta ggttgtgcgt cttcaactgc tgctttcggt gcttctgccg gttctaattc
    34441 ttccggtttt acttttacac cgtcttttgc tgtttcaatg atgtgtgctg caatttcatg
    34501 agcagattcc attgaaaagc gtgatgcgta ggcttcaatc aacattggta aattcaaacc
    34561 gctgacgatt gcccatttat ctttgtgctc ttcgaataat gtgtttgatt gattgaacgg
    34621 tgttccgccc cataaatcaa ctaagaataa cacttcgtct tgattatcaa aagaagcaac
    34681 cgcttcttcc aatttggctc tcaagtcatc tggaccttca cttggtttca aaatcacggc
    34741 tttgacgttt tcttgttcgc caaagatcat tgaaccggat tgtaagattc cttcagcaaa
    34801 ttgtccatga cttgctagga taatccctac catatttata cctcctattt acttttgttg
    34861 taacaaattc aaaaatatta ataattgcta accattacaa cagaattatt tgaccttttc
    34921 caacaaccat tcttgctaca cctttttgat tttcgctagt agttccatca aaaggatttt
    34981 cctgtcagca ggtacttttc gaatctctaa ctccacttct tgactgttca agtaacgaaa
    35041 ggaatcaata tcttgttgat ttactgaaac aaaattggta atcattgatt tgccttcagt
    35101 aaaactcata ccgccgatat tcaacgactt caactgtaca ccgcagcgga caaccgcttc
    35161 aacatcttgg ggattggtga ataaaagcat cacttttgct cgtgataata atccactatg
    35221 atagatttga atcattttct gcacggttat tacatttgct gtgatccccg gcggtgcagc
    35281 ttcttttagt agaaactttc tcaatttatc ttgagcgact acatcgctga tcacaatgat
    35341 acgttcaaca cctgtttgct tcgaccaaac tgttgcgact tgcccgtgga ttaaacgatc
    35401 atctatacgt gccaatcgaa tatccatctg caaaactcct tcttcctcac tacttcacag
    35461 aaacccctat catttctgtg tgaacaacca cctcctagca cacactacta taagcaaaaa
    35521 gcgtgccaac ttttttacga tacactaatt gaatttaaag gattcttaac caatacacaa
    35581 aaacgataca ctataaaagt gtatcgtttt tgtgtattca tggcacagtg tattcttttt
    35641 tatctaactg attatggact agacgtagca gataatacat ttcttctatc ggcactttta
    35701 cttttatctg atcttccaac agaacaactg aggctgccaa ttggctataa agaggatcat
    35761 tcgttgtatt tgtcaattcg ttttcttcca cagtcaatgg ttcattccga ataatacgtt
    35821 cgatcattcc tgctgtgtgc atcactaggt tgattttgaa tccatattct tcatcgccga
    35881 ttcccgtcgt ctgagagatt tgtgtagaaa attgccacat tggatcgatt aacttactac
    35941 cattaataaa gatgaagtta tcagagataa actcaacaca cgtattttta gctgactctt
    36001 catctaggga gatgaattca gtttcctcca attctgattc catcagcaac tgatccaaaa
    36061 tgacttcgat attttgatca ataaaacgtt ctaaaggaat aaatggggct tggatattca
    36121 aatcagtaat cccagtagta gctatgatct gatattcttt ttggataact ggcagttctt
    36181 ctttcacttc cacaatcgac aaagctagaa cttctaattc tgtttcttga cgcttgttta
    36241 ccgctcgttc gatcaattct ttgattcttt gtgctgtacc ttcgccagat gcgcagatag
    36301 ccaaaacagc ttttttctta tgaataggtt ttgcttcctg aacagattct acagaagata
    36361 tttctgcgta acctcgaaaa tttttcaatg attcatgcaa catatctaga tctgtcccta
    36421 atactgagac ctttcgtaca gtttccaaaa ctagagaagt tgttaccata tcaacagtac
    36481 gtactggaat tccagtgtct ctttgaattt gcgaattaaa cgacgcaagg gaccccatat
    36541 ctactaagag tatgaccccg cttccttcat ctacttcttg tacattgcgt tcaattcggt
    36601 ccaatgctgt tttcggatcc atatccaaag gcatgtctac tgctcgtacg ttctctgcat
    36661 ctaacaactg ttggacaact tggaccatac tgcttgctgt gctgtttccg tgagctgcaa
    36721 cgactacacc gattctgccg cttgcttgat ttgcccgtaa cgagacgagt agaacggtca
    36781 agtaatagtc ttcactttct ggaatctcca cttgaaaata cgtttcgata taactacgca
    36841 cttctttcgc aacagcatat tcatctggaa aatcaaaagc catctcacgg atgttatcat
    36901 tcgtatgccg ttcttctcct aagttgattt ttttcaaaaa agaactgata tgcaagctca
    36961 tagcataaat aaagttttgc tgaaactcat atggtaaaga tcttttgacc attacataaa
    37021 tctgattggt cacttctatg attttcggat ctacaaattc tgctaatttg ctgtctgcat
    37081 taaaagaaaa accatggtct ttatagaaag acttcagatg gatgttgata tctgtagaaa
    37141 tgaaatgatt gattgcttct tgatccaaac catccgactt cagtaaagct gctttatctc
    37201 cgatgatatc atatagatta taaggtaact cataggagtc cgattggatt ttcatgatcg
    37261 tatcatttgg tgctacagtt atctttggct ccaatatttt agaaagctct gccattgcac
    37321 tgcggttgct tgctaattga atcaatccgc tacgaatccc ttctgtcaaa tcatcgattg
    37381 tgatcgagat ttcgggtgat tgcatatgat ttagaaatcc tcgtgcacat actaactgga
    37441 tatttgactt caattgtcct atatttccat aggtcacact gccgatcaat gcttttgcta
    37501 catcttctgt caaagaaatc tttcgctgcg tgcggtttgc ttcgtgggca atcatgattt
    37561 ttactaaatc gactttctca tttgcaggac gtttttcaaa agccggaagt ttgatattga
    37621 ttgggatacg ccgaacaaaa gtttctaata gagaagaacc tgggtcttct gtggtcgcac
    37681 ctacgatccg tacatttgct tcatgggatt tcgttgtttc cccaagacga ctgtaagttc
    37741 catgatccat aaagtaaaag atcatttctt gaccttcagg cggcaaccgg tgaatctcat
    37801 ccaaaaacag cataccgcca tccgcttgat cgataatccc cattttttct tcatctgcgc
    37861 ctgtaaaagc accttttgcg tacccaaata aatgactcat caaaagctca gggttattgg
    37921 cataatcagc acaattgaaa acaatcagtt ccgtttcctc atctattaca tgatgcgctt
    37981 ttgcaaaatg atacatcgcg tgtgcaaaat aagtctttcc tgatccggta ggtcctgtaa
    38041 tcaaacaatt taatcctctt ggcggataca aaatcgcagc ttttgcctgt tccactgaat
    38101 ttttcatgct gccgttagct ccaatgattt ttgcaaagat atccttagta tctatttgga
    38161 gtgtattttt ttcttcagag accggcgttt ctttgtaact gactacatgt tttgtaactg
    38221 gctttacgtg cgtaacgagt gtttgatata tgtaacgtac tggtcgtccg tctgtcttta
    38281 tcagttttcc ttcgcgcact agctgattca gatctttact gctatttgtt cgctgaatcc
    38341 ctaacacttc agcaatttcc tgtgttgtta ctccttgatc atattctgcc tttgttaaat
    38401 gagcggtttt ttctttgaca tacatataaa ttcggtcaat gcgttttgcc aatggtatcg
    38461 ctccttttct atagactatt gtacttaggc ttcaaaacat aatactatgt atttatttct
    38521 gtttcgtcaa ctatttacct gattttgttt tgttgataac acgcggatag atagaaatgt
    38581 tcaatttaaa agaacgaaaa atcccgatac gatttttata atggaaacaa gaaaaagaac
    38641 actgtttacc atataattat ttcactgata gcaaacaata tagcttccat caattgaaaa
    38701 cgatagttat caaaacatcc tgattccaaa ttggcaaaaa aatattaagt gtaaaaagac
    38761 aaagtaaact tcgtagcctc cactacaact tttattttta taaacaaaaa aggctgtgac
    38821 aaaagtcttg tcacaacctc tttaatcata agattcaatt aagcttcttg agctactgga
    38881 taaacagaca cttgtttttt gttacggcct ttacgttcga aacgtactac gccatcaact
    38941 tttgcaaata aagtgtcgtc tccaccgata cccacgttta cacctggata gatttttgta
    39001 ccgcgttgac ggtaaagaat tgatccacct gtaactgttt gtccatcagc acgtttagca
    39061 cctagacgtt ttgattctga gtcacggccg ttagatgtag aaccgccacc ttttttgtgg
    39121 gcaaataatt gtagattcat agataacaac atggtctgca cctcctattt atcattaatt
    39181 gttttaattt ggatatattc tgagttttca gcttctatcg attgtaatcc taataaaaga
    39241 ttttctaata gaatctcagc aatattgttt tgttcttgtg tcattcctga agtgacttcg
    39301 gtataaagat aaccgccttc ttcttcattc atttcaacaa tcggctcaaa accggctaat
    39361 gaggcaatcc cgttgacagt gctgatcgct aacgcagaaa cacccgcaca aacgatatca
    39421 cttccataag gaccagcatc agcatgcccg gtcaaagtga atgaaacgat ctgacctgcg
    39481 tcatttcgct taaatgttcc tttgatcata tataggcctt ctttctcaaa cgctattaag
    39541 cgttgatcgc gtcgataacc acttttgtat atggttgacg atgaccttgt ttacggtgtg
    39601 aatgtttttt aggtttgtat ttgaaagtaa cgactttctt ttgtttgccg tgtttttcaa
    39661 cagtaccttc aacagttgca cctgcaacag ttggagcgcc tacttttgta gattctccgc
    39721 ctactaagat aacttcgtca aaaacaactt tttctcctgc ttcaacgtca agtttttcaa
    39781 catagattgc ttgtcccact tcaactttta cttgtttacc accagtttta ataattgcgt
    39841 acatgctata aagcacctcc ttctgatata cttagactcg ccatccgaag tgacagcata
    39901 tgctgtgctt attacttgtt ctgagcggtt gtagctgtgg agtccacaat tacaacagta
    39961 ctagcttacc agaacagcaa ccgttagtca actaaattta aaatcaaaaa gtaaaacaaa
    40021 aattcaagca atacttctgc ttttctatcc tcacagttct accaaataaa atagtcgcat
    40081 tgtgtttctt ttattcttat aataaaagaa aaaggaggag cataaatgga atacccaaaa
    40141 gaactgatac aagaagcttc tttaagaatg aacggccgtc ctgtagaagg atttatcgct
    40201 gggcaaggac ctcttcatcc taaactgatg ctcgtgggag aagccccagg gaaaacagag
    40261 attgatacgc atgttccttt tagcggtcaa gctggaaaag aattgatgca ggcactttct
    40321 tcaactggct taacacgaga agaggtgtat atcaccagtg ctgtaaggag ccgcccgtat
    40381 cgcgtgactc atcggatcaa taaacggaca caacagtcag aaactgttta tccaaatcgg
    40441 acacctactc gttccgaagt atttgcgcat gcaccaatat tggattacga actgatgaag
    40501 gtccagccaa aattaattgc gacacttgga aacattggtc ttcaacgttt acttggcaaa
    40561 gaatatacgg ttaccaaaaa tcatggtcaa ctctatacag ggcctgtctt acaattaaca
    40621 gaacaaaagg atgcctacga atggtcagaa aaaaactatc gaattcttcc actttttcat
    40681 ccagcagcta ttttctacaa tcgctccctt gtacctgtta tccatcaaga ctggcaagta
    40741 ctagcttcac ttttacagca atattaaaaa gaacagacaa gaccataaaa tcttcagttt
    40801 acagtcttgt ctgttctttt tcattgtttt aatggctaat tgcagtatat ttcgcatgta
    40861 aagctgtcaa cgcatgagca actgtttgat gttgatgcgt gaatgtatgt tcccacaagt
    40921 gagaaagttg tttttctact tctggctgca aggaagtata gataatcgtt tgtcctcttt
    40981 gctctgcttt ttcttgaaat tcatcaaaaa ctgcacgtgc cgtcaaatca atacttggta
    41041 cgcctcgcat ggagaaaatg acaccatcgt gatgatttag ttcttccaat ttatttttga
    41101 gtttttctga agacataaaa aacagtggac cactaatata cactactgtc cagttattca
    41161 gcttttccgt ttctggcagc cccattcttt gccaatcgat cgtttctaat gaaatcgtga
    41221 tagcagcgct tttgacaaca aaaaatacac agccgccgat caccccgatc acgatagcaa
    41281 tacttaaatc aaagataact gtacaaatca tcgtaaggat aaacaataga actgctgacc
    41341 aatatttatt agtaaatagc tccttgatcg tttcccattc attcatacgc caagccgtca
    41401 caatcaaaac acctgcaagt gctggcattg gaatctgtga catcactggc gcaaaaacaa
    41461 gcatggagag cagtaagaca actgcatgta tcataccagc taatctagtt tgtgcaccag
    41521 atttgatggc cacgcttgta cgggcgatgg ccgcagtagc aggaatcccg ccaaagaaag
    41581 gaagcaacag atttccaatt ccttgcgcca ttaattcttg attgctatct aagggcttat
    41641 ttgccattcg gccagccgaa gcaccacata gcaagctttc aatcattcct aataaagcaa
    41701 tgctaatagc tggaactgcc acttgttgca aggcagaaaa gctaaaatct cccatattca
    41761 aacgattact actgatcaat gtttgtggaa tcttacccac agttgctact ggtaaatgga
    41821 acaatagcat aactgctgtt gccaaaatga tagccagcag cgaactaggc attcgtttcg
    41881 cccatttttt tggatagata aacatcccta aaatgactag ccctcccatg atcaaagagg
    41941 ctgtgtttat cggaaatcct atcgtttgat aactacttag tttttcaata acgtttgtac
    42001 cttccgagtg aacaccaaat aaattatcga tctgacctaa tgcaataatg atagcaatac
    42061 ctgaagtaaa ccccgtaatt acgggtgctg ggataaaaga agtcagtgtt cctaatcgga
    42121 aaagtcctgc tgcaagtaaa aatagacccg ctaaaaaagt agcaagcaat acaccttgca
    42181 ttccctttgt tgccgcaata gacatcaaga tagctgccat cgcacctgtc ggacctgata
    42241 tttgataaaa accgcctgat aaaccgccaa taacgatacc ggcaatgata gccgtaatca
    42301 aacctgcggc cgcatcagct ccgcttgata ccccaaaagc caaggctaaa ggcaatgcta
    42361 cagcagctac tgtgatccca gccaaaagat ctttctgaaa tcttgaaaga tcataacctg
    42421 aaaattcttt ttttagtgag gaaacataat ttttgaacac ctatctaact cctttttcat
    42481 gaaattttct aaaataccat tcattagaat atcatatttc atgagataaa gtaatatttt
    42541 tcacaaagtt tcctgttaga taaattctta ttttttctat atttataaga aaatagaaag
    42601 caatgccaac agagctagcc caccttgttt gagtaaaatc caacgatcgc tagtcaaact
    42661 gccatataat gcaacaaaaa aaatagatat taatagcata cttgtgattt ctttagatgc
    42721 ggaagaaaga taagctccat aaagtagtcc cactcctaat agtccattgt acactccttg
    42781 gttcttaaac aatgtttgaa cagattttct gcttaactct tctttactca ttttaaaaac
    42841 cctacttgtc gtgtcagaat cagttctcac cgtttcgata tacatgatat aaaaaaattc
    42901 aattgcaact agagtcacta aaattctaga tagtatcgtc atatcttctt cctcttttct
    42961 gatgagtttt atttttttgc tccttataat tgtactcgat aaggatgttt ttttatttta
    43021 tttgcctttg aaaacaaaca atattggcac aaaaaacaaa gaaaaaccgt tagaaacctc
    43081 agtttctaac ggttttttag cgtcacagga gggattcgaa cccccgaccg tccgcttaga
    43141 aggcggatgc tctatccagc tgagctacta tgacaagaat cagcggaaca tcaataatta
    43201 tagataatta aattgcttct gtcaatacga aatagttatt taattagcaa ataatgaatt
    43261 attttggccc aactactaaa tcaatctttt aaaacgagac attctccttc agctgttcta
    43321 aggttatcaa ttgaaaacag gagctacatt gacttcatgc tactcctgtt tatttagatt
    43381 tcctgtatcc tttaattgac ttacaaatat agaaactttt acatcaagtt cgaattgagc
    43441 cctcgattca atcgattatc tacaggctgc cataatcatg tatcacaact cgattgtttc
    43501 tgattccttt gtcattaaga tcacaccatc acctaatggc aagattgtcg atgtcaaatc
    43561 gggatgatcc atcacaacac gaagaaattc atttaattta cgatggatag ctcgtttgcc
    43621 acggggaatc tcttcatctg ggagcaagat cgttcctcct tggaagatgt catctaccat
    43681 caaaacgcca ccttttctta acaaacgtaa gcattcaggt aaaaattcaa tatattttga
    43741 ttttgcacta tccataaaaa taaagtcata cggaccagac aactccggca agatatctgc
    43801 tgcttgacct tcaagcaaag tcactttatc tgtcagtcct agacgttcat agtttttttt
    43861 agctttctcg atcatgacat cgaaacgatc aattgtcgta acatgagcgt tttctccgat
    43921 tgtcgtggcc atgacacttg atgaaaaacc gatagctgct ccgatctcca agattcgttc
    43981 aggctgaagc tgtccaagta aaaatttcaa aaatacaact gtttcatggg gaataactgg
    44041 tacttctgct acatgagctt cttcttccag tttgcctagc tcacccggta attttttttg
    44101 ctgggtacgc ataaaatcca atagttcttc tttgactaca ggacgatgca tcatttcatt
    44161 gcgcatacta ctcctcctct tcacacatta tacgaacaac tgactaaatc tacaagctgc
    44221 atgagtgttt tctcgtgttt catgttcaga agagtcgttg gatctctgga atacatttat
    44281 gtttttaaca ttcttacaaa aatttctgtg ttcgtctccg cttctttttc aactctacaa
    44341 aggtattcca tgctgtgatt cctgttaggc aaaaagcaat cgtaaagaat aacccctgaa
    44401 tccatgcaaa tgccgttttt gaaaggacta gtccaatata tggaatctga aagatgggac
    44461 taccgataat atttctcttt tttactactc caggctcaat ataagtcttt gtatcagctt
    44521 ttgtataaaa atcttcgtgg ttgttttcaa cggctattac gcgacgcgtc gagatttttt
    44581 ttcctgaatt ttcataatat gtaataatat ctccgactaa aatctcattt gtatttttcg
    44641 gtcgtacata tatcagattt ccttgtgcat aatatggact catttctttg tcctcaataa
    44701 tataaggagt caaaccaaaa attcggggaa ccaagacgac gagaagaata agaattaaaa
    44761 atataatcaa cgttatagaa cttataaaag ttattttttt gtaatcaggt atcgttttca
    44821 acttctttct ttattcaatc cttcttaatc atataataac gataaatttt ttcaaattat
    44881 agctgtaaaa atgtttccct ttaataatta ta
//
LOCUS       DANKBF010000019        40131 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000019 DANKBF010000000
VERSION     DANKBF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            138..866
                     /gene="sfsA"
                     /locus_tag="PWY89_001073"
     CDS             138..866
                     /gene="sfsA"
                     /locus_tag="PWY89_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2066124.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            882..1895
                     /locus_tag="PWY89_001074"
     CDS             882..1895
                     /locus_tag="PWY89_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2066125.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            1895..3172
                     /locus_tag="PWY89_001075"
     CDS             1895..3172
                     /locus_tag="PWY89_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2066126.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3235..5937
                     /locus_tag="PWY89_001076"
     CDS             3235..5937
                     /locus_tag="PWY89_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2066127.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6089..6406
                     /locus_tag="PWY89_001077"
     CDS             6089..6406
                     /locus_tag="PWY89_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2066128.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6436..6756
                     /locus_tag="PWY89_001078"
     CDS             6436..6756
                     /locus_tag="PWY89_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2066129.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            6864..8324
                     /locus_tag="PWY89_001079"
     CDS             6864..8324
                     /locus_tag="PWY89_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2066130.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8392..8613
                     /locus_tag="PWY89_001080"
     CDS             8392..8613
                     /locus_tag="PWY89_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066131.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            8644..8826
                     /locus_tag="PWY89_001081"
     CDS             8644..8826
                     /locus_tag="PWY89_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL2066132.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            8826..9239
                     /locus_tag="PWY89_001082"
     CDS             8826..9239
                     /locus_tag="PWY89_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL2066133.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9362..10543
                     /locus_tag="PWY89_001083"
     CDS             9362..10543
                     /locus_tag="PWY89_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2066134.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10614..10778)
                     /locus_tag="PWY89_001084"
     CDS             complement(10614..10778)
                     /locus_tag="PWY89_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066135.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            10932..11005
                     /locus_tag="PWY89_001085"
     tRNA            10932..11005
                     /locus_tag="PWY89_001085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10966..10968,aa:Arg,seq:cct)
     gene            complement(11074..12213)
                     /locus_tag="PWY89_001086"
     CDS             complement(11074..12213)
                     /locus_tag="PWY89_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2066136.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(12512..13147)
                     /locus_tag="PWY89_001087"
     CDS             complement(12512..13147)
                     /locus_tag="PWY89_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066137.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13260..13895)
                     /locus_tag="PWY89_001088"
     CDS             complement(13260..13895)
                     /locus_tag="PWY89_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL2066138.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(13929..14390)
                     /locus_tag="PWY89_001089"
     CDS             complement(13929..14390)
                     /locus_tag="PWY89_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL2066139.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(14520..14951)
                     /locus_tag="PWY89_001090"
     CDS             complement(14520..14951)
                     /locus_tag="PWY89_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2066140.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(14969..15289)
                     /locus_tag="PWY89_001091"
     CDS             complement(14969..15289)
                     /locus_tag="PWY89_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2066141.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            15588..16364
                     /locus_tag="PWY89_001092"
     CDS             15588..16364
                     /locus_tag="PWY89_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL2066142.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            16379..16582
                     /locus_tag="PWY89_001093"
     CDS             16379..16582
                     /locus_tag="PWY89_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066143.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            16598..16936
                     /locus_tag="PWY89_001094"
     CDS             16598..16936
                     /locus_tag="PWY89_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL2066144.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            16923..17102
                     /locus_tag="PWY89_001095"
     CDS             16923..17102
                     /locus_tag="PWY89_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066145.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17145..17615)
                     /locus_tag="PWY89_001096"
     CDS             complement(17145..17615)
                     /locus_tag="PWY89_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066146.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17702..18400
                     /locus_tag="PWY89_001097"
     CDS             17702..18400
                     /locus_tag="PWY89_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL2066147.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18578..18919
                     /locus_tag="PWY89_001098"
     CDS             18578..18919
                     /locus_tag="PWY89_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066148.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            18912..19583
                     /locus_tag="PWY89_001099"
     CDS             18912..19583
                     /locus_tag="PWY89_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2066149.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            19589..20281
                     /locus_tag="PWY89_001100"
     CDS             19589..20281
                     /locus_tag="PWY89_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066150.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            20278..21027
                     /locus_tag="PWY89_001101"
     CDS             20278..21027
                     /locus_tag="PWY89_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066151.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            21039..21308
                     /locus_tag="PWY89_001102"
     CDS             21039..21308
                     /locus_tag="PWY89_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066152.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            21314..21478
                     /locus_tag="PWY89_001103"
     CDS             21314..21478
                     /locus_tag="PWY89_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066153.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            21471..21770
                     /locus_tag="PWY89_001104"
     CDS             21471..21770
                     /locus_tag="PWY89_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066154.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            21770..22072
                     /locus_tag="PWY89_001105"
     CDS             21770..22072
                     /locus_tag="PWY89_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2066155.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            22112..22477
                     /locus_tag="PWY89_001106"
     CDS             22112..22477
                     /locus_tag="PWY89_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066156.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            22967..23107
                     /locus_tag="PWY89_001107"
     CDS             22967..23107
                     /locus_tag="PWY89_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066157.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            23104..23286
                     /locus_tag="PWY89_001108"
     CDS             23104..23286
                     /locus_tag="PWY89_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066158.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            23299..23493
                     /locus_tag="PWY89_001109"
     CDS             23299..23493
                     /locus_tag="PWY89_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066159.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(23526..23738)
                     /locus_tag="PWY89_001110"
     CDS             complement(23526..23738)
                     /locus_tag="PWY89_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066160.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            23863..24066
                     /locus_tag="PWY89_001111"
     CDS             23863..24066
                     /locus_tag="PWY89_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066161.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            24063..24335
                     /locus_tag="PWY89_001112"
     CDS             24063..24335
                     /locus_tag="PWY89_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDL2066162.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            24336..24521
                     /locus_tag="PWY89_001113"
     CDS             24336..24521
                     /locus_tag="PWY89_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066163.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            24508..24855
                     /locus_tag="PWY89_001114"
     CDS             24508..24855
                     /locus_tag="PWY89_001114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066164.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            24815..25291
                     /locus_tag="PWY89_001115"
     CDS             24815..25291
                     /locus_tag="PWY89_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL2066165.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(25437..26165)
                     /locus_tag="PWY89_001116"
     CDS             complement(25437..26165)
                     /locus_tag="PWY89_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HDL2066166.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            26213..26491
                     /locus_tag="PWY89_001117"
     CDS             26213..26491
                     /locus_tag="PWY89_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066167.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            26493..26879
                     /locus_tag="PWY89_001118"
     CDS             26493..26879
                     /locus_tag="PWY89_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066168.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            26876..27256
                     /locus_tag="PWY89_001119"
     CDS             26876..27256
                     /locus_tag="PWY89_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2066169.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            27368..27820
                     /locus_tag="PWY89_001120"
     CDS             27368..27820
                     /locus_tag="PWY89_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL2066170.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            27817..29544
                     /locus_tag="PWY89_001121"
     CDS             27817..29544
                     /locus_tag="PWY89_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2066171.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            29608..30795
                     /locus_tag="PWY89_001122"
     CDS             29608..30795
                     /locus_tag="PWY89_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2066172.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            30767..31336
                     /locus_tag="PWY89_001123"
     CDS             30767..31336
                     /locus_tag="PWY89_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDL2066173.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            31349..32713
                     /locus_tag="PWY89_001124"
     CDS             31349..32713
                     /locus_tag="PWY89_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2066174.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            32715..32996
                     /locus_tag="PWY89_001125"
     CDS             32715..32996
                     /locus_tag="PWY89_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2066175.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            32974..33300
                     /locus_tag="PWY89_001126"
     CDS             32974..33300
                     /locus_tag="PWY89_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2066176.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            33290..33628
                     /locus_tag="PWY89_001127"
     CDS             33290..33628
                     /locus_tag="PWY89_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066177.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            33618..33983
                     /locus_tag="PWY89_001128"
     CDS             33618..33983
                     /locus_tag="PWY89_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2066178.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            33990..34616
                     /locus_tag="PWY89_001129"
     CDS             33990..34616
                     /locus_tag="PWY89_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2066179.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            34616..35080
                     /locus_tag="PWY89_001130"
     CDS             34616..35080
                     /locus_tag="PWY89_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066180.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            35275..37584
                     /locus_tag="PWY89_001131"
     CDS             35275..37584
                     /locus_tag="PWY89_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2066181.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            37596..38300
                     /locus_tag="PWY89_001132"
     CDS             37596..38300
                     /locus_tag="PWY89_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2066182.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            38297..>40131
                     /locus_tag="PWY89_001133"
     CDS             38297..>40131
                     /locus_tag="PWY89_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2066183.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        13788 a         6417 c         8155 g        11771 t
ORIGIN      
        1 cgcatagaca tattctttct tctttatttc attgtaccga ataaaagcta aaatttctct
       61 aagactttcg actggttttt ttactgtttt tctacaaatt atgataggat acatagtagg
      121 aagaaaggtg acataatttg aagtatcagc aagtggaaat agcttttttt attgaccgcc
      181 cgaatcggtt tatagcacat tgtatgaaaa aagacggaga aataattatt gcgcatgtca
      241 aaaatacagg tagaggaaaa gaggtcctga taccaggcgc tgaagtggct attgtatttg
      301 ctcctggacc taaacggaaa accgcctatg atctgattgc tgtaaaaaag aagggtgact
      361 ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat tgatggtatt ttagacggca
      421 cgatcctttt accaggatta actggtccat ttacggtata caagcgtgaa gtaacttatc
      481 ttcactccaa atttgatatt tatttagaga cggtggtcgg acaaaaagca tttgttgaag
      541 tcaaaggtat gacacttgaa aataaagcga ttggtgcttt tcctgatgcg cccacactca
      601 gaggattgaa acatatagga gaattaacgt atgccgcaca agatggttat gccgcttatg
      661 tcttgtttat tgctcagttc gaacatcttc atctggcaac gatccatgaa gaaatgcaac
      721 cagcactagc tgatatggtt cgacatgcgc agcaaagcgg agttcaaatc ctcgcctata
      781 attgtcaagt atcggcagat gaggtaactg tcaagcaggc cattccattt gatgtagata
      841 tcccttttga agatcctata caatagaaag aaggtaaaat aatgatcaac ttaggtatta
      901 tcggaacaaa ttggattact catcaatttg tccaagcagc tttaagcaca aaaaaatatg
      961 aattgacagc ggtatattct agacatttgg aaaaagcaca ggaatttggt tctcattatg
     1021 aaggggatat cgaattcgca acagatttga agactttttt caatattact catatggaca
     1081 ccatttatat tgcttctccg aatagtcttc actttagtca agctaagcaa gcaattttgg
     1141 caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac gccaaaagaa atggatgaaa
     1201 tcatccattt ggcaaatgaa cacaatgtgt atttttttga agcagcaaga aatatccatg
     1261 aatcaggttt taaaaagatt gccgattact taccattaaa agacgagata atcggtgcaa
     1321 atttcagcta tatgaaatat tcttcgcgct atgaccaagt attagatgga gaagaaccga
     1381 atatcttctc gactcatttc tcaggtggtg cattgatgga tttaggtgtt taccttgtat
     1441 atgcagctgt tgcttggttt ggaatgccaa aagaagtcca ttatttccca agaaagattt
     1501 ctactggtgt agatggtctt ggatggggga tcttacgtta tgagaatttt gatgtatcga
     1561 tccagccagg taaaatcggt gattcttatt taccgtcaga gatttatttt gactcaggaa
     1621 cgctagtgat gaatggagtc aatgcaattg aacaagcaca atttcatgat cgcgcgcata
     1681 aagaaatcat tgatttggcg gtaacagctg cagaaaatcc aatggcagat gaagcagagg
     1741 attttgccaa tgtcatggct catccgaaag atccagcttg gggattgttg tatgaagagt
     1801 ggatcgagct agctagaaat gtgaaccaag tgatttatga tttaagaaaa aatggcggca
     1861 tcaaattcga tgcagataat gaggaagatt tttaatgaac gaatttgtac aagtatttcc
     1921 cgaaaattgg cagaaaaaat atgaggataa agggtttatc tcaccttctc ttattcaacg
     1981 agcagttttt cagccactaa gtaataaaca aagtatagta gcggtttctc ctacaggttc
     2041 aggtaaaaca ctggcttatc tttggcctct actactaaac gtggaaccag gtgaagcaag
     2101 tgcgttggtc atttttgctt cttctcaaga actagctatt caagtagcag atgttgcacg
     2161 tgaatgggga aaagacaaag agttgaaagt ccaatctttg gtgggcggag ccaacgtcaa
     2221 acgtcagatt gagggtttga aggaaaaacc agaaatcttg attggtacac caggaagaat
     2281 attggaattg atgaaagcaa aaaaaataaa agcacatcaa gtgaaaacaa tggtatttga
     2341 tgaagcagac cagctatttg acgctgggaa cagccaaatc attgatcaaa ttcttcatca
     2401 agctccaaca gagtatcagc tagctttttt ttcagcgaca gcagatcgtt cccttgaaag
     2461 cattgaaaaa atcactggga aaacgtttga gacaattgac gtgacagcag aggacgattc
     2521 tcgaaaagga ctgagacatt actttttacg tgtaccgata cgaaagaaag atgactactt
     2581 gagacgtctc acgcatatag aagattttca tggtctgatt ttctttaatc aactgactga
     2641 attaggcgta atggaagaaa aattgctgta tagaggtgta ccggtaggaa gtctggcctc
     2701 tgatcagaac aagctactcc gaaaagcagc gctagaacaa ttcaaaaaga aaatggtcag
     2761 agcacttttg accacagatg tggcggctag aggtttagat attacaggtc ttccttatgt
     2821 agtgaatgca gaagttccgt taagtgaaga agcttatttg catcgttcgg gaagaactgg
     2881 tcgaatgggt aatgaaggaa tagtaattac actcgtacaa gataataatc taagagattt
     2941 aaaacggctt ttacgtccat caaatataac acttgaagag atttttttat acggaggtaa
     3001 gctgcaaact gagcagccaa ccaaagaaga tgcagctcct gcagtaaata aaaagtatgc
     3061 gaaaactttt tctggaaagg agcagaaccc agaatataac aaagaaaaaa taagaaaaaa
     3121 agttaaaaat aaatcaaaaa aagagagaaa taaaggagca aggcgcaaat aagcgccttt
     3181 ttatgctatg attatgaaaa cgggaacaaa attggttgcc agaggagggg ttatgtgaaa
     3241 gaacgtcaag aagagatctt gcagctattg acagaaaacc aacaaggatt gacagcaagt
     3301 gatgtagctg agcgcttaac tatcgatcga agcaatgcaa gccgatattt aagtgaacta
     3361 tacaaggccc atcatattgt aaaaaccgct ggccgaccag tcgtttactc tttgccaacg
     3421 gaaaagtcaa aatctgatga agtgcatgtc gactcttcta cgcaagtgac ttttgaaaca
     3481 ctcgttggag aaaatgattc tttaaaagtc agcatacagc aggcaaaagc tgcgattctt
     3541 tatccaccgc gggggttgca tacgattatt tttggagaaa ctggtacagg gaagtcgatg
     3601 tttgctgaat gtatgtatca ttttgcaatc gattcagaga tgctttctgc agatgcgcca
     3661 tttgtttctt tcaattgtgc ggattatgat caaaatccgc aactgctctt tggacacatc
     3721 tttgggatca aaaaaggggc ttacacagga gcagctcaag atagtcccgg tctaatagcc
     3781 aaagcagacg gcggtatatt gtttttagat gagatccacc gtttaccgcc ggaagggcag
     3841 gaaatgctgt ttactttcat tgataaaggt atttaccgtc cgcttggaga aagtgcacaa
     3901 gtacatgaag catctgttca gatcattggg gcgacgactg aatcttcaga aagtttctta
     3961 tctacgttca atcgacggat tcctatggca atcaccttgc cgaatcttgc ttcacgttca
     4021 ctggatgagc gttatgaaat catctctctt tttatcaaac aagaagcgaa tcgtttgaat
     4081 caacgtatcg atatcgaaaa agaagcgatt cttgccttca tgctttatga tgcggaagga
     4141 aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg caaaatcttt tttacattac
     4201 cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag aactttccct tcaagtacaa
     4261 aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg accgattcat ggacagcaaa
     4321 agtcagtatt tgacatttga accaggattt gctgatgttg tctggtcaca agatccagaa
     4381 cgaaacatgc aggtctataa cgatattgag gaaaaagtca cttctctttc cgaaaccggt
     4441 gtggagaaca ttgatctaga gaccctcatt tcaaaagatg tggatgctta tttccagaca
     4501 tatgtcaaag aattgactaa aagttcgatt cccaaagatc ttttgccaga tgaaatctgg
     4561 cggttaacca atcaattgta tgatgtggct gaaaaacggt tagaccgtat ttataatgaa
     4621 aaagctcgtt ttgcttttgc tttgcatctg caaagtacat tggatcgtgt gagagaaggt
     4681 catatgatcg tccacccaga tttgaataac gtacggaaaa attttaagag ggaattccag
     4741 gtagcaatcg atctttcaac gatcattgaa gaagaagaac aagttgagat tccttttgat
     4801 gaaatcggct ttattagtat gtttctttct atcaatctag gagaaagtga gcctgttaaa
     4861 gaaaacaaag tagatgttct catattgatg cacggcagac agactgcttc aagcatgttg
     4921 gagatggcac aagaactatt aggaacaaaa gtagggaaag cttttaatat gccactggtt
     4981 atggaagttc aaacaatgta tgaagaagtg ttacaatatg tcaaaaacta tcaagagcat
     5041 ttgacgaatg gcctactctt gttaacagat atgggatcat taaacacctt cagtgatttg
     5101 atttatgaag agactggcat tcggacgaaa gctatcacga tgacaagtac catgattgtg
     5161 atagaagcga tacggatggc aagtgttgga agaagcttag aagatattta tcaaaatatc
     5221 cagctttcct ttgaatcgat cgtacgtgat cagttcaaag cggaaaagtc tgaaacttta
     5281 cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg aaggagtagc tgccaaatta
     5341 tatcagcgta ttatgccagt gattaatcaa aataaggtag aactgatcca gatgcagttt
     5401 ttagaacgtg aatcattcaa aaaacacatc gatgccttat tggaagaata tgagatcaaa
     5461 gcaattgcgg gaacagttga tgttgattat caaaatatcc catttttttc agcctatgac
     5521 gtttttgatg atgaaaaact aaatatttta aagcgtatag ctgatgatga gatcccgaca
     5581 gctaaaattg tcgattcact tgctggatca ttaagcgatg tcttttctat tgatgaactg
     5641 atccgttcat tgcaaaagac agttcagcag atccagaccg atttgtcgat tatcgtggaa
     5701 cctggagtag atactggaat cgttatccat ttagccttca tgttagacgg attgatcaaa
     5761 caagaaagcc atcggcaatt tgagaaactg tctattttct caaaaattta tcgtttggaa
     5821 atggatatcg ttcgggcaaa cttgatggca ctagaacgaa aatatcaggt aaaaattcca
     5881 gaagatgata tcgcatttct tacacaaatg tttcttgaaa ataaaataaa aaaatgaaca
     5941 cactaatcat actgtttaca cactaaacat ctgtgtgcgc actataaaaa agagctaaaa
     6001 agcatgaaaa cgctatttca gctctttttt ttattggcat gcttcttgcg ttatataaaa
     6061 gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa acaatcatgt tagtttgttc
     6121 agcagggatg agtacgagtt tattggtcac aaaaatgcaa aaagcagcag aagaaaaagg
     6181 gatggaagcc gatatttttg cggtctctgc ctcagatgca gataataatt tagaatctaa
     6241 gaatgtcgat gtattattat taggtcctca agtacgtttc atgaaagctc aatttgaaca
     6301 aaaacttgcg cctaaaggta ttccattaga tgtcatcaat atgcaagact atggcatgat
     6361 gaacggagaa aaagttcttg cacaagcaga aaatctaatg aaataaatca agtatgcagt
     6421 tgggggtata acaacgtgga agatcaacaa aatctagaag ccatcatggg gttaatcatg
     6481 tttggcggta atgcaaagag cgatgcaatg gaagcgattg cggctgctaa aatcggtgat
     6541 tttgagttag cggataggaa aatcgctgat gcagaagaat cacttataca agcgcatcat
     6601 gcacaaacag acatgttgac acaagaagca caaggaaatc atatgcaagt gacattattg
     6661 actgttcaca gtcaagatca cttaatgaca tctattgcgt ttactgattt agcaaaagaa
     6721 atcatcgaac tttatcgtcg cgtatacagc gaataaatac aacttctttt ttgaacggaa
     6781 gaagaaaaat ttttgaacgg attttttatt ttgttataag gagaactctc tttatataaa
     6841 aaacttatag gaggaagtaa aaaatgaacg gactatcaag ttggatggag aaatacatcc
     6901 tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt tgctttgcgt gatgctttta
     6961 tcggcatact gcccgcaaca atggcgggat cagtcgctgt aatgatcaat gcaattattc
     7021 gtgatttacc gcaacagttt aacagtacct atgctgacag tttagcagca gacaaaggaa
     7081 tctttgccgt attgaatgtc atcatcggaa tcaatggttt tgtctggaac ggtacactag
     7141 caatcgccgg attgatcttt gccttttctt gggggtataa tcttgcccga gcttatggag
     7201 taaatgatct agctgggggg atcgttggtt tagctacctt gattcaaggt atttcctttg
     7261 gtccagcatt atcagcagca ttagatgtga atgttccttc aaatgtcgca gatgcaatca
     7321 atagttctga gattggtgca acgattgcag atggcacact gtcagttgga ttatggggtt
     7381 ggctaaaact agatcatttg aatggaaacg cttattttac agttatgatc atgggagcta
     7441 tttcagtcat tattttttgt aaattgatgc ttgccaacat tacgatcaaa atgcctgatt
     7501 cagtaccacc agcagtatct aaagcatttg cagccatttt accagcgacg atcgctttat
     7561 acgtcattgc tattatcaat tatatcgtag gtcgtgtagc tgatgggcaa ctgatcattg
     7621 atttggtgca aaaatatatt gcagaaccat tcctaggatt atctcaaggg ttgggagcag
     7681 tattgatcgt cacagtattt gttcaagtat tctggttctt tggtattcat ggatcaaatg
     7741 tattggcacc agtgctagag ggaatctggg gacaagcaca actaatcaat attgatattt
     7801 tccaaaaagg ttataacgga aaaactggta caccagccgt gttagatgca atcgaagatg
     7861 gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt tgcatggttt ggcggatcag
     7921 gcggaacgat cgtcttactg atagctatct tgattttctc taaacgtgca gattatctaa
     7981 cagtagctaa attatccatt gggcctggta tcttcaatat caacgaacca gtgatgtttg
     8041 gtctgccaat cgtattgaat gcaatcatgt ttatcccatt cttgattgct ccagtagtag
     8101 caacagcaat tggatggaca gctacttatc ttggcctagt cgctccggta tcgcagcaag
     8161 tcacatgggt agtaccgccg ttcttattgt cattcttagc aacaggagcg gattggcggg
     8221 caccgatcgt aacattagta tgtatggtcg tgactttctt gatttgggca ccatttgtca
     8281 tctctgcaaa caaaatggac cctgccgcag gcgaacaaca ataagatgaa cgtgaggagc
     8341 gggacgcttt aagtttcgtt cctcataatg aattttcgag gaggaactga aatgattaca
     8401 attgccagtg caatgcaaca agatgaaatc aaagaattgt tagaaggata caatgaagaa
     8461 ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc tgttctttga aacaagtgca
     8521 gaagatccgg aagttgcggc taaaaaagca aaggaactaa tcaaagcaca gccttgggga
     8581 acagtccttt attttcaatc aacagctgtt tagaaaatgg tttaaaaaga taggatggtt
     8641 gagatgataa aaaacggttt atttttatgt agtattggtt ctttgatcgt gttgtatagt
     8701 ttgaatccac attcgcaaag gtatgatttg tatagtatgg tcacaggtct ttttctaaca
     8761 ggattaggaa tctatttctt catgaagggg aaaaaaagag aagaagcaaa ggagaaaaat
     8821 aactaatgga aatcacgaaa aaattaaata ttccagcatc gtttttttac gataaagtta
     8881 tggactctgt ccttttcgat gtacgtaaag cgaccggcaa atcagtaact agaaaacagc
     8941 tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa ccgggctcgc ataaaaatcg
     9001 aagaagtcat tgaaaaccag tcttataagt ttcgtacatc gacaacgaag aatgaattcg
     9061 tcgttcatta tcaaatcaaa ccattggacg attcgacctg tgaggttcat tacacagaaa
     9121 aaatggagtc ttatggcatg cttcaaaagt tgaatgatat gcttttagga acaatcttga
     9181 tgtattttaa acggagacgt ttcaaaaaaa tgctagatat gatcgaagca acgtattaaa
     9241 tcaatgaaac atggtactat ttagttattg aaaaatgaag taagctagaa ggaatttttc
     9301 ataggaagat agatctttcc tgcgaatcct cactagcttt tttgtaggaa ggggcagcaa
     9361 aatgacaaac aaagtaagag agctaggtgt atcttcgatt gaggaaatgt ttgatttagc
     9421 cacttatgca tttaatgggg cagatacacc tgaaaggcga gaacgttttc gtacacttgc
     9481 ggagaattct tggaattacg gattttttga tgaggaggat cggctaacaa gtcaagtcat
     9541 ggcgacccct tttccagtga atttttacgg acaagagtac ctaatggccg gcattggcta
     9601 tgttgcttct tatccagaag caaggggaca aggcgggatc aatcagatca tggaaaagat
     9661 ccttgaagat tgccgtgatc gtaaagtaag cttatcttat ctggcgccat tctcctatcc
     9721 cttctatcgg cgctatggct atgaacaatc atttgataaa atcagttatc aactaaacag
     9781 tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata aagcgtatgg attttgaaga
     9841 tgcaaaagct gcaatcagaa gaatttatga acagatgccg gaaaaccaac gagctggtct
     9901 gaagcgcgaa gactggtggt atacgtataa attcaagcaa aaaggcaatt accagtttgc
     9961 tttgcatttt gatgataatg aggaagcaac gggatatatc gtttatcagc ttgcaacgtc
    10021 ttcctttatt attgcggagt ggggattttt ggatcatgat gctttttgca gccttgttcg
    10081 cttcgtaagt tctcacaatg gtgcttttga aacatttgaa tattcatgcg gctacggcgg
    10141 taatgatcaa aattatctat tagaaaatcc atttgcttct gtaatgatca caccttatat
    10201 gatggcgagg atcgtagata tttctttgtt tttggaaaat tatccattta aaaagagaca
    10261 agctgcattt gccctacaaa tagaagaaga tcattatgcg aaatggaatg aaggaatcgt
    10321 ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa gtagaacaaa caaaactccc
    10381 aatcgttcga ggaagtatcc aacatatcac gcagctatta ttagggtatc gtaaagtgga
    10441 agagttggta ttccaagaaa gaatacaggt gacaaaagaa tatgaagaaa cgttaagctc
    10501 aatcttgcca cagcaacagc caattcttaa tgactatttt tgataaaaaa cgggtgatga
    10561 caatattttt gtcaccaccc ttttgttttt catttggcat ttgaaggaaa atgttaaagt
    10621 aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt ttgttttagg gttttctttt
    10681 ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat tttttttaga tgaatctaag
    10741 cgttctgcgt atactttttc ttttggctta tcattcattg gaaaggacct ccctacatta
    10801 tttcattcta cctctagttt atcatctgtt aaggactagt ggaaacgttt tgcctaactt
    10861 ataagctgat ttttttagca gataagcttg caagtaaaaa acaaacaagc tacaattgat
    10921 ttattgaaat tgttcctgta gctcagtagg atagagcaat cgcctcctaa gcgataggtc
    10981 gctggttcaa ttccagtcag gaacgtataa ataacccctg tatcctttgc ggtacagggg
    11041 tttgttttgt atttgtgcca aaaatgtgcc aaactagtta tcatttttat ataaactatc
    11101 gaacacagta ttgatttttt ctttatcctg atcttcaagc tctcgaacaa tatgaagata
    11161 agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc taagtatatt
    11221 tactccttga taaagtaaaa tagatgcatg tgtatgcctt agtccatgca aagtaaattg
    11281 cttttcaatc cctaattctt ttaatttttt gcgcaaatat ttgctgacgg cgtttgatga
    11341 aactaatcca ttttttaagt tgaaaaaaac aaaattatga gggtttttga tttcaaaatt
    11401 ctcgaataat tctttttgat tgattttaaa cttttttaag agatcaatcg tatgtttatc
    11461 aatggaaatt ttcctatttg aagtttcgtt ttttgttttt ccccattcat ttaatttata
    11521 attccatgtt ttattaatag tgatgatttg atcttcgaaa tcgatatctt cccatgttaa
    11581 tcctagaagt tcagcaaatc gcattccagt ggcaccagca actaacacga gcataggaga
    11641 agagtactgc gcagaaaggt tttcttctac gagtttcatt aaagatttaa attgatcata
    11701 atctaaatat ttatcttctt ttggcttcaa tgaatctttt cctttgatta ctgcttttct
    11761 agttggatca aacggaatta atccttcttc tacggcatct ttcaacgacg ctctaatatg
    11821 gttattaaac ttaataacgg tagattttac atggtcttta gcatacttat ttaaaaagcg
    11881 ttgatatccc actctatcta aatcagaaat caataccgct ggcatatatt tctttatgtt
    11941 cattaacgta tcttcgtatt ttctataagt gataggagat actgttcctt ctttataaag
    12001 ttgcatccaa tcctcaaagt aatctgataa taatagattt ttgcgatccc ctttaaggcc
    12061 tttgttcaag ttatattcta attcgttggc ggcatctttt gcttctcctt ttgtcttaaa
    12121 tcctgatttt cttatcttcg tgtattttcc gtcatctttt ttgtatgaaa tttcgtattg
    12181 ccaactattt ccacgtttga ctaatcttgc cataattgaa tcagctctct ttctctgata
    12241 caataggaac tataaagaag cccattgttt gggttttatt attttttgca catccttttt
    12301 gcttgccggc gcaaggatgt gctatttgtc gttttaatgg tataattgga tgtgtattag
    12361 atcatccaat cagaggtgtt tattatgatt aaagtgattt ttaaaagaat aactgaactt
    12421 ccagagttta gattatttgt tgattattgg tatgtctggt tttttattgt tgcattgctg
    12481 ataggtctag atgtcatatt agaaagaaaa cttaagtaag agttgagtta ataaattgaa
    12541 ttcctaaatg agatattttg tagcccatat tttgtttgcc gacatcacct aatactatgg
    12601 agctaatcgt tttttctaac agaccaaaat cagataatct atttaagcta ccatctatta
    12661 gctcttgtga atagccagac atgctgatat ttccagtgaa attttcgttt ccacgactca
    12721 ttaagcatgc taacaattga atctcaatcg atgttaattg attcagtaca agaagataat
    12781 actcctcgtt atcccatgaa gcgttgttaa ttcgaagtaa acaattctta taaagatttt
    12841 tatacatatc tattttattt tgtgcctttg atttttctac ctcgtcattt atcccttcga
    12901 atattccaat gagttggtcc ttgttctcta agttgaaaac atcttctgga atttgatcgc
    12961 gaatgttttc taatgattcg tttaaatttt tgtagaattt ctcgatgcgt ttgaaacgct
    13021 tctcgttttt agacccaaaa taaagtgact gcacaacgcc accaatataa ggaattgctc
    13081 caataccgcc ttcaactatt aattcaagat attctttttt ggttagtttt tcattgctat
    13141 tatccatagt tacctcctat tttgtcattt cctttacaag cattgtgact cctgatggca
    13201 ctcttttgta taggttttat tattgtgcac aatctaactc ttagtggggc gtggttcatt
    13261 tattttttac ttactttact attaatcata tcccattgag cataggattt ttctatgttc
    13321 gatcctcttg aactaatttc tcctgtaaaa gttactttat cgccctttgt aaaggtatca
    13381 gcattgttta agtccttcgc aaaaattaca tatgaaactt taggtgttgc agaaatatca
    13441 ttccaacttt tatcatcgta ctctttgtct gctataatgg ctattctagt agtcattgat
    13501 tcgataacag ttccttcaaa agtatatgtt ttaccactaa tatatttagt aaaagctgta
    13561 ttttgctcag tagctggagt aatagaataa taactatcta agaaagcacc ccagtcatga
    13621 ttatttgtct ctaaaaattc ttcaaattga gttaggcttt tattgctgct gttagatgta
    13681 gtagtttgag tatcagatga tgagctttcc tgtttgttat tgtcatttgc ctcgtttgat
    13741 gaggaaagcg agaaacctac ggtagtaaaa ataatcccga taataaggag aatcataggt
    13801 gttttcttat ttgttttttt gataaatctt acaattaaca tgattaaacc tataataaat
    13861 aaaacaatac cagtaaaacc aaatataccg tccattaata gatcctcttt ctcttttgat
    13921 attgaatatt ataaaccaag caattctttt ttcttaaaat cgaattcttc ttgattaata
    13981 atcccttcat ctaacaactc tttatacttt ttaatttcat ctgcacttga aatttgtgtt
    14041 tctttggaat tattatctaa tatagattgt tgcttctcta tatatttttt taaattttcc
    14101 atttcattcc aatatttttt tgtaaacata acggtgtttt catctttcgt ggccgctaat
    14161 atacctccgc gactttcgtt tccgcctagc aaggtaaatt gtatataacc attactcata
    14221 cctggttttt ttaattgtac ggctgatata tttttaaagg ggatagtttt ttctccttta
    14281 ataccttgat taactaaatt tataaatcct tttctaataa ttgaaataga atcatcagtt
    14341 actttaatca aggtttttcc tggagatttt attaaaatct ctttttccat tttttcttcc
    14401 tcatttctgt gatatgattt tttttgtaga agctcatata tgaggaaaga gcccgtgttg
    14461 cagcacgggc ttttttaatg ttttgctgaa atcggttttt ttaataattc ttgatgtatt
    14521 catacatatt gccttgcgta agaatatttt ttcttaaaat agcattggca gacagcataa
    14581 caagtgtatc tgagcttatt ctgaatagaa taatactcca taaatttttc gagattaaac
    14641 tgagattcat cagtcagttc gttctcaata tagatattta ataaaattag aatagctatt
    14701 ttatctgctt ctgtttcaaa ttttgagtga aaagttgtag aagtatcgta caaaactgaa
    14761 aattcaaaat gagaggcgct gaaatgtgca agttcatgag ataaatgaaa ggcttctgca
    14821 gtttcaccgt atagattttc attcaaaaaa atgattctgg gttttggata gtagaaacca
    14881 ggctctttca tctccatata aactactttc aaattgtatt cgctcaacat ttctttcaat
    14941 ttcaaataca tacaaaccat cactccaact attcattttc ctctaaagct ttagcaattg
    15001 caatcgcttt acgcattgtc tccttagata tttcttttcc atcaaaagaa aaaacagtat
    15061 cgtcttctga taaatccaca tgtttagggg tttctctttc ttctctacct agaaggtagt
    15121 ctacagagac atcgaaatag tcagcaatcc gacttaattc atctgaatta ggagtattat
    15181 ttttccactt tgctagataa ccgttagaat atccaaaatt aatttctaac tgccgtatag
    15241 atatcttttt cctcttcgcc aagtctttta ttatttcata ggtattcatc gataaaacaa
    15301 cctttctaaa tgtttacaaa aaaagtttag aaaaatacgc agaaataatt tgactaattc
    15361 tgagtaatga gctatactat atcttgtaaa cagatttaac aactaaaaag acaacaaaaa
    15421 acactattga tatgtaaatg cagaccgcca agaaagcttt aaaatcaatg tttttatgtc
    15481 ttatttaatt atgctcttag tatagagtat tactcagata ctgtcaattg aatttagaaa
    15541 aaagttgtta aatttgttta cgaatataaa agaaaggaat gagaaatatg aacacaccgc
    15601 aaatttttaa tttcgaacaa aacgaagttc ggacaatttt agtaaatgat gaaccatatt
    15661 ttgtaggcaa agacgttgcg agtgttttgg gttattcaaa cactaaagat gctttgtcgc
    15721 ggcatgtaga tttggaagat aagatggggt cgcgaatcac gacctcaggt caatcaagag
    15781 agatgacaat catcaacgaa tctggtttgt acagtttaat cttaaaatca aaacttccct
    15841 ctgccaaaaa atttaaacgt tgggtaacaa gtgaagtgtt gccagcaatt agaaaacatg
    15901 gaggttatct aactccagaa aaagtagaag aagctttgct taatccagat acaatcattc
    15961 aattagcaac tcaactaaaa gaagaaagaa ctggaagatt aatcgcagaa caaaagattg
    16021 cagagtacga acctaaaatc tcctatttag atagcatatt atcttctaca gattcagtaa
    16081 caattagtca gattgcagca gattatggga tgtctccaca acagatgaat aaattacttc
    16141 ataaactagg tattcagaaa aaagtcggta accaatggtt attgtgcaaa aaacacatga
    16201 accaaggata cacaaaatct catacaactg agatcccgaa agccgatggt ggcactaaaa
    16261 ttgtaatgaa taccaaatgg acacagaaag ggcgtctatt tatctacgaa ttactaaaaa
    16321 aagaaggata ttaccctcaa atggatttag aggaaattgg ttagaaagga gttttagtat
    16381 gactgacatt gcagaaatca ctcaacgaga tagagaaaaa atcaaagaat atgtcgaaag
    16441 ttcaaagttc ttaacttaca ccatgcttgc tgaaagattt ggaattagca aaagctactt
    16501 atctttaatt ttaaacggta aaaagacttc tgcagaagca aacagaatta tagattcgat
    16561 tatcactatg tacgaattgt aaagggagga acagctaatg caatatctag aagcaaaaat
    16621 cccaattcca gaaggctatg taattatctc ccaagtggat tatgaggagt taaaaaaagc
    16681 tgatgatact ggtagatgga tgacgttgcc agaagtgcta gaacggatta acagaaaata
    16741 tgattggttt acttctagag ttttaaagaa cccaagatat agaaatatta tcgatatcga
    16801 gaaaaacaaa aatggatttg tctattatcc agttgaagga agagacacat atctattttt
    16861 aagaagtaaa acacttgaat ttttagaaac aaatttttcg gaaatcttaa ggaggcaagc
    16921 ggatgggaaa atttaataga gcgctagtgt tcagcgcacc gctaatcatc tacgctttag
    16981 gactttgggg aagcaggcaa gcgttgatag gaacgattgt ttacatggtt tggattttta
    17041 tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag ggggctgact
    17101 aaaaatgaat agaaaagaga aactagaatg tatattacta ttactcagtt taattctttc
    17161 actaatttct ctattgggaa gtttgtattt ttgatatcaa caattttgtg gatttgggtt
    17221 ctatttagag taaatatcat ttttgtttgg tgattaagga gtttattaaa attaattttc
    17281 cctacttcaa atgcaaggta acaagatata gcacttttag gaggaatatt tagcggtaca
    17341 gcttcagagt atattctatt atcatcgact gatcctcctt tagcaattaa tttcttaaat
    17401 ggagtagctg accatctgcg gtttaagtta gcggatgaaa gttctaaatt aactaacata
    17461 gctggttctg ttgaatagtt agaaatgatt acttttgtat aaatcatatc tgcagcgaag
    17521 taagaagcat taagttcaac ttctagttga gggcgcattc cttttatttt tatccctgtt
    17581 aaaaacgtac tcaatataaa acttattatt gacatccatt gaaaaattgt taagtgaaaa
    17641 aaattcaaaa taaacaccac cagtttttaa ctaaattata ccaaaaagga gagaagaaat
    17701 aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg actacgagct tacaagtggc
    17761 agaaagcttc gagaaaaaac atcaacatgt tttaagagat attgatgcac taaaaaaaga
    17821 tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat atggcagaaa
    17881 tcgacgagtt ttcttcatta gtagagatgg atttttcttg ctggctatgg ggtttacagg
    17941 aaagaaagct atctacttca aacaaaaata cattgaagca ttcaacgaaa tggaagatgt
    18001 tattcgcaag aatactgttc ctcaaacaat tgaagatatg atgatctatc aattagaaga
    18061 aatgaaagat gttaaaaaag atgtttccat gcttaaagat actatgcgaa ttagcggaca
    18121 acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc tagggggaaa
    18181 agaaagccga gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt
    18241 taaacgtacc ttttcaatcc caagatatgg cgagttacct cgtaagagat tcgatgatgc
    18301 tgtttcattt attgaaatgt ggttgccaga aactgcgatc cgtatggaaa ttgatcaact
    18361 gaacagacaa cagagacttt ttggtgatga caatgaatag agctgaagcg ctaagaatag
    18421 ggacggcaat tgctaattgc tggtggaaat actataaacc aatcatccta agccaacaac
    18481 atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg actccgccgg caagcaaaga
    18541 gtcacaaaga aaacacatca taaggagatt ttagcatatg gaaaaagaac tttccactct
    18601 agatcaatat ttgattgatc ctgattgggg caagccgaaa attgaggaaa caagtggtcg
    18661 aaaaatcaga cgaaatcttt tgacgaatga agaactagct tgggatcaag atgatttagg
    18721 caaccatgta actatttggg atcatgttta tcttatccat ctatcgaagc attcgaataa
    18781 acctgaatat atttacgtca tcgaagatgg cttgattgat gcgctagaag agtacgacag
    18841 agataacttg attgatatct cttattacgg accaggtaag aaatacattg ctgaaatgga
    18901 ggcagaattt gatgagtgaa atcaaaggga caacgaactt tgaaaaactt tttagtcgta
    18961 agttaaataa aattctcaag aaaaaaggaa attttgatta tttatcttgg gctcacgcgt
    19021 gggagattat gaaaaagaat gatccacagg caacggtaac tattaatgag tacaaacact
    19081 acagggttgt ttctggaact catcaagact ttcttgttga ggaatataaa ccttttctta
    19141 tggatgaaac tgggacttat gtatctgtct cagtaacggt taaaggacac acggaaaccg
    19201 aattatttcc tgttttagat tatcgaaacc aaccagttgt taaaccaaat gcaatgcaaa
    19261 tcaataactc attgaagcga tgctttgtga aagcattggc tctacacgga ctgggattat
    19321 atgtatttca aggggaagat attccaacac cacctagaat cgatacaaag aaattaaaca
    19381 tgctagagac gattctagaa gctttcaatg agcagatggg taaagatatg accaaaacct
    19441 taatcgaata tgttaatgag cagacagata aattagggct cttagctgat aacgttgaaa
    19501 ctattgaaca gttaagctat gagcaatgtg ccttgatgga gcgagcaata gcagctaaga
    19561 gaaaagaatt agataagaag tgatatgagt gtttaaacca ttaatcgatt catattcagc
    19621 ggttctgaaa aagttcaaag gaaaagacat aagcgcaacc atcaatgagg aagtgaacat
    19681 tgatcgacta aagacgatgt atgacggcta cgatggtgat cgagtcattg aaattcgttt
    19741 tattgatcct agacgtttca ccgtacagca acgaaacttc atctatgcgc tgataggcga
    19801 tatttttatc gatacaggca tgccaacgga cttctggaag gaattcttct actttcgttt
    19861 tgaaggtgtc acagggcgca aaataagcct gaaagacgaa tcgaatacaa ctgtgagtga
    19921 tgccaatgtc ttagcaaata tcatcttaga tttcatcttt gaacatcata ttcctttcaa
    19981 agaaggctat gagattttac ctgcgaatca agaatattac ttctacaaat gcatcacaaa
    20041 aagagtctgc tgcatctgtg gcaaaacagg agctgacatc gatcactttg acaaagcgct
    20101 aggaagacga aagcgcaaag aagttgatca tgcagagtac acatttgcag cactctgcag
    20161 aatccatcac acagagaagc acaaaatagg tgtgattaat ttcaaaaata aatatcaaat
    20221 caaagggatc aaattaaacc agaaaacaat caaaaagtta aatattggag gggaaaaatg
    20281 acagaacatc gaagttatta tgcgattata ccagccaacg taaggtacga caaaagactt
    20341 aaaccaaata ctaagttgtt atacggagag ataacggcct tgtgtaatga aagaggcttt
    20401 tgttgggcag gcaatgagta ctttgcagat ttatatggtg tgaataaaga gaccatatcg
    20461 cgatgggtaa gtgatttgat taagtttgga tacttgaatc gggaaatcat ttacaaagag
    20521 ggtaccaatc aaataatcaa taggtaccta cgaattaatc aataccctat tgacgaaaaa
    20581 cgcaataccc ctattgacga aaaagtcaaa gataataata catctattaa taatacattt
    20641 aataatacaa aagaatatat aagagagtta ccgccttcga aaaaatcgaa ggctaagccc
    20701 gtccgtcata aatacggaga gtataaaaat gttcttttgt cagatgagca gatggagaaa
    20761 ctcaaaacag aattccctaa tgattaccaa gagcgaatcg aacgactgtc agagtattgt
    20821 gaatcatctg gtaagactta taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa
    20881 gaaaaaagtg aacctaagaa cgcaagcagt ggatacaagc gcacaggaag acgagagaag
    20941 cttcctgaat gggcaatcga ccaagaagcc tatctcaaga aaaaagcgct agaacgagct
    21001 aatagacaat caaaagcacc attctaagag gtggaaaaat gaagatcgat tatctagaac
    21061 taattaatga aatagcgaat tataaaaagg gcgaggaatt agacgtcctg agagacgtat
    21121 atgatcaact cgaagaagct ggaattgaag gaattaagaa tgatcgttcg agttggagta
    21181 aactcagata ctatttcgca ctctatatcg atggaacgca attaagaaat ttagcatata
    21241 ccaaattact attcattgat tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc
    21301 aggtgtaaac aagatggacc taaagacatt tacagcacag atcgaattaa tgcatcaaga
    21361 agctttaaga caaagtgtgt cgtacgaaga caagtggctc aacacatttc atggcggacg
    21421 tgagagcgca cttgatcaag tactcaaatt actgaaagga gaatgtcggg atggataaga
    21481 aagcggcaat gcagcgaatt atcgaattga cttattcaga agattggcag gaagataaaa
    21541 aaatagttgc agaagtccaa aagctcggta aatcaatgtg gactgaaaag cccaaacgga
    21601 gaacgccgag aaagattgca atctggcatg gtgatcgaat tctagtaacg ggtaccgcta
    21661 aacagttatc tgaaattact ggtctgagca aaaatattat ttgggataga gctaggagct
    21721 tatggattga ttcaaaagga agacagttta ggtatgtgga ggagaaataa tggacgaact
    21781 aatcgcgaaa gtagagcagt gggcaaaaga taagggattg gatcaagcgg atttcagcaa
    21841 gcaaatgtta aaaacggtcg aagaaatcgg ggaagttgcc gcttctctag ctagaaaaga
    21901 tgaacatggt ttaagagacg gaattggcga tgtagtagta accttgatca tcttggcaat
    21961 gcaaaatgat atggatttat acgagtgctt ggattttgct tatgacgaaa tcaaaggtcg
    22021 gactggaaag atggtcaatg gtgttttcgt taagtccagt gacctgaaat aattccatta
    22081 atggaagaaa taactgaata ggaggataga catgattgat gcaaaacaag tgcaaaaaca
    22141 gaaagatgga atgttgatgt ttgaagcata tgttctacca tttcttaatc aatttgaagt
    22201 tttggaatgc tctgccagcg gagaagaatt ggagtatgtt gtaattcgtg aaaccaaaga
    22261 aaacgttcag aagctaaacg aatttctttg cactattaac tgttgggata tgatagctcc
    22321 aggattttta tgtccagcta tgggagaatt tttggaatat tgtcgtcttg aagatgcagg
    22381 cactttggat ttagcttatc tggtatacaa ctacctaaac atcaacacag atcatttgtg
    22441 gtttggcact gccgaaagaa aatgggtagt ccgctaacca ccaatatcaa tgaaaaaagg
    22501 cggtttcctg aaagtggaat tgccgccatt gctgtaaggt tctttacgcg actcaaggtc
    22561 acagaaatat aaatgcaaat ctcttttgaa gtgatccaaa gctctcggaa aatggaagtt
    22621 ttttgtttca aagaataaat gagatttgcg tcaagaacct gagagttatt tccaaaaaag
    22681 cttccaaaga accgaatgag ctaaggacat catgtgtaaa cacataatat agctctcctt
    22741 tcacggctaa tcataacatg aaaaatattc gatgtcagta ggaaaaaagg tgaattatga
    22801 aaagaatatt aaattatccc ggcagtaaca gattctgcaa ttaaactccg aagctgatgt
    22861 atcagtatca actgtagaat attatgagcg tggagaaagt caagcagtca caaatgtaaa
    22921 aatcgataac gtcaacggtc ttatctgttt agatgaaggc gatagtatga atgaaattgc
    22981 tgcagaccat gaattatcag aaactgcttt aattgatcaa atcgaattag ctagacaata
    23041 ctttgttcca tatgcagata cctggtccaa gcataagaat gacatcattt ttccagagga
    23101 acaatgagag caactgatcc agtaattatc cttgaggaag ccaaatttat ttggactcac
    23161 gaagagatag agcaagcacg cttgctcttt tctcaaggag ttaagccgag caaagtagct
    23221 gaaataatgg gtcaaaagat tcttgatgtc ggattgcttt tgctccatct agcagaaaaa
    23281 atttgatttg aggtgaaaat gatgattcaa cttgcaggca tccaaacagg gaaaatttat
    23341 ttttctggag aaagcaaaag cgaagccaac caatggctat tgaaaactta cacgaacaat
    23401 aagaagctaa ggaaaaaata tcctgatttg ttgctaaagg atgatcagat tatgccggaa
    23461 ccgatgattt tgacccgtaa ggagggaagt tgaaactaaa aaaccagccg tgtgggctgg
    23521 agttgctatt tctttttaat aatgagtttt tctaaattta attcattctc acgagtttgc
    23581 gtccaataat cagaataata ttttaattgt ttaagagtag gaatgtcgtc ttcggtaaaa
    23641 ttagaaaata attctggaga attatctttt tttaattgtt ctaatcctga ttctttaaaa
    23701 cgttgttcaa cgagttttac taaattgttg atattcatga taaatcacct ttttaaattt
    23761 aatttcgatt tgattaatcg atacaagtat tatatcaaat aaaaaagctg atttaggggg
    23821 tcttacaaaa acactaaaat tttttcaaaa agaggaggaa cggtggtgaa agtcagagaa
    23881 caattcaaga tagtgagcga cgaaggtaat gtttacaaaa taaagatcgt taatattaat
    23941 gaatatcgag aagcagatca caaatatttg gttgacttat acatgaacga taaatatttg
    24001 ggagaaggat tttgtggaga ttcacttttt gatgatgagc gaattttgaa ggaggaaaca
    24061 gaatgatcca cgaactaaaa atactaccag aatattttga agcagtgacg agcggacgca
    24121 aacaattcga gatccgcaag aatgatcgtg acttcaaagt tggtgatcag ttaatactac
    24181 gtgaatataa aaaatacatt aaacatgaat tacctggact aacgagacca acatttagtt
    24241 acactggtga ctcatacaaa gcagagatca cttatattac tgattacatg cagaagtccg
    24301 gatatgtagt gttggggatt aaaggggtga attaaatgaa agtatacatc ttagctatca
    24361 cagagggcac atggatgttc ccagtcggtt ccggaaaaat ctataaatca aaaacagctg
    24421 cttataaagc atttgaaaaa tacaaaaaag aaaacggtgg cggcactaac gcaaagattc
    24481 tagtggctga taactggcat gaagaaggtg aacgtaattg atttacctgt tcattttcgt
    24541 ggtactaact atttttatgc tagtaatctt attcaaaaaa agatgagtaa agacgaacga
    24601 aaaattctgg ccaactttta tttagtatta aaaatcgaac aaaatgtgtt ttcggaaagg
    24661 agaattgtat gggaaaaaca aaatcaaaga ttaagaagaa aaaacgacgt ttggaacaaa
    24721 aggcaatcca gaatggaacg gctaagaaaa aataaaaaag ctaccccttt cgagatagca
    24781 gcgcaatact attctaccat aaggggtggc gtttgtgaga tttcattggt taaaagatta
    24841 ccaagaacta gatgagcaga ttctttactt aaagtggaat cttaataaaa gtaagcttga
    24901 attgaatcga tgggtctgtg gtgatttagc aaacgtccgc atcgaaaaga attcgagaac
    24961 ttcttcttta gaagagaata tccaaaagat agaaaatgaa ttggaattgt tgatcgagca
    25021 aaaagaagaa atgttattgc tgatagatag cttttctggt atcgataatc aaattgttaa
    25081 gatgaaatat gttgatcaaa tgagcttaga agatattgct gaatctgttg ggtatagctc
    25141 atcgtatgtc agacagcgac atgcagaaat tagaaagaca ttgaactttt tagatgagta
    25201 tgaacacaga caagctgacc gattgaaaaa agaaaacgaa attgattttt acaatagcga
    25261 gaagtacaaa gaacaactct ctttattcta aaattacact gttcactaaa tgttcggtca
    25321 ttgtagctat gtaaacattg ttagcagcat gatattctat tagtgtcaaa aaaatatgaa
    25381 agagccaaga tatcccaacc gttttattaa ttggtatctg tggctctttt attcttttaa
    25441 ttagtagttg gcataaagtt atttccattt ataaattgat cagctaaatc agcatcataa
    25501 tcagccgcga taaattgcgc tgctgattta ataaatgcat ccatatcatg aacatcttta
    25561 ttagtatgtc tacgaacata gtgcgtttca tcattaccaa tccaagtagc agctttggct
    25621 aatctttgga gtttaggaaa atcattcaaa taattttcaa ttgttttacc taataacatt
    25681 ttttttattt tatcttcatc ctctgaattt ttagatattg cataatcttt aattagaaat
    25741 tctagagctt ttctataacc aactccagca atttgttcca attgctcaga tttagcttgt
    25801 gtggcttgag tataaatatc aacaaatgta ggagaaactt tttcaatatt ttctggtaaa
    25861 tctattttaa tcggcggacg gtaataatct ttaattaaaa tattttcaga atatccttca
    25921 tcttcattaa acgtatttat aaattgtaaa ctataaaatt tgttgcactc atcatttata
    25981 cactgtacta atacgccaaa gattctatca ttatcgaacg cactatatga tgaaacccca
    26041 aaatacacat acggagtcat tatatttccg caatgcggac aactatttgg caaataaatt
    26101 gtctctgtat tttgacgatc acgatactta atattaactt cttgaagcat taaaacacct
    26161 cgcatctttt ttataaatca taccacaaaa tatctgatag gagagtaaac aaatgaatga
    26221 ttttcatgaa gcggtactta gtattgaagt agaatcaagt ttggctaaag tttataagaa
    26281 agcaattgaa gcagagaata gtccgtacag agaaaattgg aatggtaatc atgcacatgt
    26341 gcaagtggag aatgatgatt acagaactgg aatgaatacg ttagttattt ctttattgtc
    26401 acacactcta cccaatctgc aggaaacgat tgaatggtat gagcgaatgg gcgcaaaagt
    26461 aatccgtaca aattacaaag gagagaatta aaatggcaat gatcaaaatt agaacatcca
    26521 ttagtggaac agagtattgg gattcagaaa agaaaaggac agtagttgtt ccgaaagatc
    26581 aagaaccaaa ttttgaagta aaagaagaaa aaggaatttt gagtgatggc aaatcatttg
    26641 ttgctgtcaa tggcgaactc atagctcata gcagtgaaat tcttgatagc gatggaaaca
    26701 ctgctgctaa ttttgatgga gacgaagcta ctaatgatca gtccactgaa gatacggatg
    26761 aactggacaa tatgactgca aaagaattgc gtgcatatgc taagaaacat ggtattgata
    26821 ttcctggtgc tatccgtgca aaaggcgata tcataaatct tattcgtgaa gtagaatgaa
    26881 atactgtcag tttgacggat gcacgaacaa aatagcaaag ggtatctact gtactgaaca
    26941 caagagatca agcagatcac gcaagaagaa gcagcaagca aagtctgttt atcatcatga
    27001 gaacaaacca ttctatcgaa cgcaagcatg gaaagatatg cgtcaattta tttatgaaag
    27061 agaaggtggt cattgtcagc ggtgtggtca gttcatattt ggaaagagag cacacgtcca
    27121 tcacattgta ccaatcaaag acaacgaact gcttaagctt gatccaaaca atctcatgct
    27181 attatgttca aaatgtcatc caattgttga aaacgaaacg gaagacaaaa aagtttttcc
    27241 ttcgtatttc aattgaagcc cccctattca ttttcaaaat tttttcgcgt ggggagatag
    27301 ggtagcgggg agtcacgcgc atcgttaggt caaatttttc aaaaaacaaa ggggggtgta
    27361 tacaaaaatg acgactaaag cgcaacgtaa agcgattatt gatgaaaaag taagtgctga
    27421 aaaagctcgt atcttagaaa taatgaattt gtctgatttg tacaccatca ctcttgatcc
    27481 attaatcgaa tcatacttgg atatttttga aatttaccaa cacaaatatt tattgtggaa
    27541 ggaaaaagga tttccggaga cgcaaaaatt cacgaataaa tcaggagcta ctaatcaatc
    27601 aaaacatcca ttggcacaac aagtagaaac ttgggcagat aagaagatga aagctctaga
    27661 tttattagga ttaacaaaca aagcaaaaac tggtagacta attgctggag gatcgaccgc
    27721 aagaaaagat gaagaaatta cacgcccaga agtaaagcca gtagatgaac tagcagcgca
    27781 cagaaataaa tggcgtaaga aggcaggtgc tgaaaaatga ttgaacctgg tgtaaattat
    27841 gctgatttat ttgcaaaaga agtaagaaag aaacctaaga agtatcctaa gaccgttcgt
    27901 ttagcaatag atcgctggta tcagtggaaa aaaagaaaag atatttggtt tgatgtagat
    27961 cgcgcgaatg aaatgatgga ttgggtagaa tcatttattg ttcacacaaa aggagaaatg
    28021 gtgggaaaac cgtttctttt agaaccctgg gaaaaattta tttattcttg gatttatggt
    28081 tgggttaaag aaaatgaaaa aggccaaatt gtacgcgtta ctcgtgaggc ttatgtccag
    28141 attccaaaaa agaatggtaa aacattgatt gctgtcggtt cattaggata tgcaatgtac
    28201 ggagaagggg cgttatcagt tgattgctat gcttgcgcat ctgattttgc tcaagcgcaa
    28261 tatgctgcca aaccttttgc agttacaatc ttaaacaatc caatcctact tgaaggaact
    28321 aaaatattta gggggccaaa gggaactgtt tctagtatta cttatgacta tattcatgaa
    28381 gatatggctt actcaaataa atttattgtt caaaccaaga atattgataa tatcgaggga
    28441 tccaatccat attttgtatt gaacgatgaa ttgcataaac aggagaaaat ggaacagtat
    28501 gacaatttta agtctgctca gatatcttta ccacagccat tgatgtttaa tatctcaact
    28561 gctgggaaag gttcgtcgtc tgttggaatg cgtgtatatc gcgaagcaaa agaagtgttg
    28621 aaacgtgatg ataatgattc aaactttgtt ctaatctatg agccaaataa aaattatgat
    28681 tggacggata gaaaagtctg ggaaatgtgc aatcctaact ggggaatatc agtcgattta
    28741 tctgctttag aatcggcatt taaaacagcg caacggtcag ctcattccaa agctgaattt
    28801 ctaacgaaac acttggatgt atttgtgaac ggcgcggata atttctttga acaggatcaa
    28861 gtagagccat gtttagttac cacacaagaa cttggtgatt taagtggcga accatgttat
    28921 atcggtttag atttatcacg tacacgagat ttgacctgtg tatctttaaa cttcccaaca
    28981 tgggatgaag atggtaaagc ggttcttaaa gtaaagcaac tttattttat tccgaatgaa
    29041 gatttagagt ttcgagaaaa agaagataat gttccttaca gtgatttggc tgaacaaggt
    29101 ttcgttgaat tttgcgacgg aaaaatgatt gatcaagatc agattttgca gtacatagag
    29161 gactgcatga atttatatga tattcagcaa gtgaattatg atccagcgat gagtgacaaa
    29221 cttgttgaga aattagaaaa cttaggattg gaatgcgttg aagttgctca atatcctaaa
    29281 gtattgaatg ctccttttga cgatgtcgaa cggttgtttt atgagaaacg aattcaattt
    29341 gataatccat tattccttta ttgcactttg aacgttgtag caattactaa catcaatgga
    29401 caaaaagcgc caagtaaacg tcaatcaaag aaaaagattg atgggttcgt ggcgtttttg
    29461 tgtggtcata aggaaacgat gaatcaaatg acagatattg attcagatga gttagatgat
    29521 tacctaagtt ccatctatag ataaatagaa aggcggtgaa aaaaattgaa attacgtgat
    29581 cggttatcga atgcagtcta ttcttttatg gaaaaacgtg gatatatcga ggatattttt
    29641 ggacactaca cacgttatgg tcaaagatat gtgacggatt catctatcat ggaatcttct
    29701 gatgtttatg aattagttca agacatatct aatcaggtgg cattggccac accaattgtt
    29761 attggtcccg acggcaacga agtcaaagat catcacttac taaatatttt gaagagtccc
    29821 aatgattatt tgacaggatt cgaatttacc aaattggaaa caaatacttt attgatcaat
    29881 ggtgagacat tcccattaac ggatagggat cagcttcatt tggcgtacgg tgtaacaact
    29941 aaaatcaatg aacgacttca agaagaattt gaaatgaatg gtcaaaaaat acctggtcaa
    30001 atgattcgac acattaagaa catcggaact gattcattaa aaggtgctgg aataattgat
    30061 cttgcaagaa acactctaga aggcgttctg agcgctgaaa aagttttgac ggacaaatat
    30121 actaaaggcg gtttacttgc gttcatgctt aaactagacg cccacatcaa tccaaataat
    30181 agcgcccaaa caaaaattgt caaagctata ttggatcaac tggaaggaac gcaaaatgag
    30241 agtgatcatt ctgttaagat gattcctttg ggaaaaggat attccatcga gacattaaaa
    30301 agtcctgttg atgacgcggc aattttgaat tatttaggtg tttacaaaaa agacttagga
    30361 aaatttctag gaatcaatgt tgatacgtat caatcgctga tgaagacaga tattgaaaaa
    30421 gcgatgatgt atctgcacaa caaagcaatc aaaccaatat tgaaaaacaa gagcgaacat
    30481 tacaccgctc ttttttttat gcctaattct ggctatagag tggaatggaa aattaatatt
    30541 ttggattttg ttccttactc aacaaaaaca aatattggct acaacatcgt tcgtaccggg
    30601 attacaagtc cagataacgt ggcagaaatg cttggttttc ctaaacagaa tactccagaa
    30661 acacaagcta tctatatttc aaatgactta tctaggattg gccagaaaaa tgcaacagat
    30721 gattccttac caacgaatga tcaagacttg aaaggaggtg atggaaatga agaagaagga
    30781 aattcgcacg attgacatca ccaatctttc aacacgttcc gatgaagaaa ctcatacgag
    30841 gaccattagc ggctacgctg ctgtattcaa cagtcaaaca cttttatggg atgacttaag
    30901 cgaagtgatt gttcctggtg cattctcaaa ggcaattagt aattcggatg ttcgttgttt
    30961 gttcaatcac gattggtcca atgtgctagg acgtactaaa agcggaaccc ttcgccttga
    31021 agaagatgaa cgtggtttga aattcgaagt tgatttaccg gacacaacgg tagcaaggga
    31081 cttagttaaa tctatggaac gaggagacat caaccaatgt agttttggtt ttgtgccgac
    31141 tgaagaaacg tgggactaca attctgaacc aatgcttcga acaatccatg aagtggaatt
    31201 atacgaggtt tctattgtgc ctttgccagc atacgaagat acagaagctg cattaagaag
    31261 tcgtgatgaa ttagaaaaaa acgtcgaaga aagaaaaaaa ttaatcaaaa aaattaatca
    31321 agcgctagaa gcgtaggagg aaaaatacat ggataaagaa ttattgaaaa aaatgaaggc
    31381 acgtcgtgag caacgattga ctgaattacg tgaaaaagtt gaatcaggag aattacgcga
    31441 agcagattta gaagccgtga aagaagaaat tgacagtgtt attgatgaat tgaacggaat
    31501 taaagacgaa ttaggcgcag attctggaac tgatgaaaca gacgacaata cagatgatca
    31561 atcgaatagt acggattcag acgaaagccg ttctggtgaa gacaacgatc aagaagaaga
    31621 ttcagacagt gaagatagtt cagaaaatcg ttctggaatg attactcaac agcaacgaga
    31681 tggattactt ggatcaatta agaacggatt ggaggcacgt gcaaaaatga ccaagaaaca
    31741 aaaagatcaa caactacgaa aagcatttgc taattttgta gctggaaata tttctgaagc
    31801 agaagctcga gctttaggga ttgaagctgg caacggttca gttactgtcc cagaagtaat
    31861 tgcatctgaa gttattactt atgctcaaga agaaaattta cttcgtaaat acggaacagt
    31921 ggtgcgaaca tcaggagatg tcaaatatcc aattcttgtg aagaaagcag atgcgaatgt
    31981 aaacaagaaa gagcgttcaa ctgatattgc tgaaacagct attcagtttg atgaaatttt
    32041 gcttaatcct gccgaattcg atgctttggc aacagtaact aaaaaattat taaaaatgtc
    32101 tggtgttcca gttgaagata ttgttgtgga agaattgaaa aaagcttatg tgcgtaaaga
    32161 aatcaattat atgttcaatg gtgatgacgc tggaaatgaa aatcctggtg cattagccaa
    32221 aaaggctgta gcatttaaaa aacctttaga tctaactgct tcaggtgctg ggcaaaaatt
    32281 atatgatgca ttaatcgaat ttaaaaatac accagtgaca gaagtgatga aaaagggacg
    32341 ctttattatt aatcgagctg ctttgactgc cattgaaaaa atgaaaacag atgatggatt
    32401 tcctttgttg cggccattca cacaagcaga aggtggaatt ggttaccaat tagttggcta
    32461 tcctgtggat tggacagatg cagcagataa aaagggtgaa ccagacacgc cagttttata
    32521 ttttggcgat ttttctgcat ttaaaattca agaagttatt ggtgccttgg aaattcaaaa
    32581 acttgttgaa aaattctctg gtaaaaatca aattggattc caaatttaca acttgctaga
    32641 tggtcaattg gtttattctc catttgaacc ggcagtatat cgctacgaaa ttacaaaacc
    32701 agttggtggt taagatggaa gagcaaacta aagaattgtc tttagaggaa aaattcaaat
    32761 cacatattca ttttgaagag ggcatggatg attctttgct ctctttttat ttaaatatgg
    32821 caaaagatta tgtcaaaaca gcaactggtg gccaacaaga atatcttatt ttgatggttg
    32881 ccggcattgc ctatgaatat agagtttcag aggatgaact cgacaaagct atgaatgcca
    32941 tgacgccatt tatcgtgcaa ggagcgattc aaaatgccga agagacagac caataatctc
    33001 agatggaaag ccgaattgct agacatcaaa acaggaacag acggaaacga tcgtccaact
    33061 acaatttacg aatttaagcg tctaatattc tatgaagaac tcggtgtgac ttctcaagaa
    33121 aaatatttat cacagcaagc caagacagac gttgtcagac gaattaaagt cagatgggat
    33181 aaatccatca cagagaaatt aagtgcgctc aaaattgatt ctgtaacgta taacattact
    33241 cgcatttata cgaatcccga tgcaagagaa atggagttga gtttagctta tgtcgattag
    33301 ctttgatgaa ttgaaaacag cgctgaaatc aacaaagtta gcagtgttca gagacaaagc
    33361 cagattaggg acgatgtatc catacattgt gtactcaaat gtgagtaaca gcaaaaaaat
    33421 ggcatccggt aaagtctata aaaaattacc ttattaccaa gtctcttttt ttacacttgg
    33481 aacagaacag gatttagttg ttattgaaca agcgctgcaa aatgctggca ttccatattc
    33541 agattttaca ggtatacaag gtgatgagaa cgacgatacc gtaaccaact attacacata
    33601 tgtgaggtgt gtggaaaatg ccaagtaata agaatggttt ttctgaaata tcggattatt
    33661 taggaaacct ttcaaaagtt gatccgaaaa aattatcgtt ggaatcttta gaagaagcag
    33721 ctaactttta tctaaagcag ttgcttccta atattcctaa gtcgctctta aaaaagaaac
    33781 atatgcgcga tcaaataaaa gtagtagttg aaaacgatca agtgaaagtt caatttgaag
    33841 agacggcctt ttactggcgc tttgccgaaa atggcacaac aaatcaacgt gcccaacatt
    33901 ttgcaagtgg tacgtatgaa caaaacaaag aaaaaattga agaaattatg actagaaaaa
    33961 tacttgattt atgggaaggg tgattttaat tgggaaaaca agatactttt tattttgaag
    34021 gattggacga cattttaatt gcgatgatgg caacgccaga ttctgttgga actgcaccga
    34081 cttatagtga agttttacga ttgccaattg ccacaaaaat cggtgtcaaa gggaatggaa
    34141 cagcattaga aaaatgggca tccagcaaaa tgtttcgtcg tgtgtcgcga gaaacaaaac
    34201 atgaacttgc gttggatcac gtgggtattc cgatagaagt gatggatgaa atcaaaggat
    34261 tagttgctaa aagcggagta acgtttagca aaaataccgc acgagaattt ccgtacttcg
    34321 cctttggatt tattggaaat attgaaaatg gcggtaaaaa agcagtttgg tatcctaata
    34381 cacaactatc caatgtgatt gacgaagagt atgccactgc agaggaagaa acaaaaattg
    34441 acgatgtaac tgcaaatctg gtttccatcg gactgaaata caataatgtg atgtatgcaa
    34501 gttttgattc aaatagagac ggagcttctc tagaattatt taataaattc attgctcaac
    34561 ctgtatacga tgaagaacag tggaaaacat tggctaaagt tggaggtgcg ggttaatggc
    34621 tcggttatct gattatggaa ttcacgttga agacttaaaa aattctgctg ttgtaattat
    34681 tcaaggcgca gaattcccta tttcatttac tatgcaaacg atggaattta tagcagatgt
    34741 gtatggtgga gattattcgc aatttgaatc tgatatgaac gccatgctat ctaaaaaaga
    34801 aggacaaatt tcttctgcta acttatcgcc tagtgatcta aaaatcatgc gtgccttgat
    34861 ttatgccatg ctgcgtactg gtggtttaga ggaagatcca gaaactattt tcaaattttt
    34921 gggaatgagt ggagaggtgt tgtctgctta tagtacctgt atggaaattt ttgctagcca
    34981 gacatttcag gtggaagacc taaaaaaatc caagaagcca caagactttc aaaaagcgca
    35041 agcaaaaaga aagaaaaaca aaaagaatcg gaagagatag gaactccttg gagtttttat
    35101 atttacgttg ctctcactct attgaattgg agtgagagtt tctttttaaa gtctacacct
    35161 aacttgtggc tcaaatctta tttacagtgg ttacaacaaa acaccgattt tgaaccacct
    35221 caatctgtaa ctatggataa atcgccttgg tggtagaaag gagcgctaag ataaatggct
    35281 ggtaaagaat ctgatgtcgt tcttaatttt aaaacgaatg gcgaagtcag ttattcgaaa
    35341 acaatcaaag aaatcaacaa agaaatgaac ttagccgctg ccgagtacaa aaaccaagtg
    35401 tctgcgatgg acaaggatgc aactcaaaca gaaaaattgc gagcggctaa acaaaaatta
    35461 gaaaaacaat taggattggc tgaacaacga tctcaaatgc tgagagagga atatgaaaaa
    35521 tctgtcaaag aaaccggtga atattctgct gaatcagaaa aactttataa acaattactc
    35581 aattctgaaa ccggagaaaa taaacttcgt acagcattag aacagacgaa cgatgcactt
    35641 aaagaacaag gtgacgtttc tgttgataca gcaaaaaaac tccagaaaat cgaagaaaca
    35701 ggtgagaaag taaaaggcgt tggtgaaaaa atgtctgttg gagtaaccgc gcctattgta
    35761 gcggcaggag cagcaggact tgcagcattt ggtgaagttg acgaggcact tgataccatc
    35821 attacaaaaa ccggagcaac aggtgatcaa gctgatagac tttcacagtc tttcaaaaac
    35881 gttggttcaa atactcattt acctttacaa acggttgggg aagctattgg tgaggtaaat
    35941 acacaatttg gattcatgga taaaaaactg gaagattcaa ccaattatct cctacagtac
    36001 gctgaaatca atgatacaga tgtttcgcaa tcagcaatat ctgctcgaca agctattgat
    36061 gcttatggac tagaatatga tgatttgaat tctgtccttg atgtaacaac gaaaacatcg
    36121 cagaatactg gtcaatctgt agacgacttg atgcaaaaag caattgatgg cgcaccacaa
    36181 attaaacaat tagggttgag ctttggtgaa ggggtcactt tacttggaca atttgagcaa
    36241 tctggtgttg attcaagcgc agctttaagt agtttatcta aagcaacagt ggcttatgcg
    36301 aaagacggaa aatctttaag tcaaggacta gaagaactac aagacaaggt aaaaaatgca
    36361 ggttctgaaa cagaagccat caatgcagca gcagaagtat tcgggactaa aggcggtcct
    36421 agaatggccg atgcaattcg tagaggtact ttgaacctgg aagatttagc aaaaactgct
    36481 ggagaaagtg ggggagctgt aggagacaca ttcgatgcta ctctcgaccc aattgatcaa
    36541 gccgatcaag ctatgaacaa tgcaaaatta gcaatggctg acgtaggtga agcagtccaa
    36601 gtaagccttt tgcctttttt tgaaaaagca acttccttat tacaggaatt ctctaaatgg
    36661 tgggggtctc tagatcaaga tacaaagaat tggatcataa caattgctgg tatagcggca
    36721 gcagttggac cagtactaat cgttcttggt tcacttatgg gatctgttac aaaaatcgtt
    36781 ggggaagtta aaagttttat aggagtttgg caaggacttg ctggtttatt tggaatgtcg
    36841 ggaggctggt ttgcattagc agttatagct attggcgcat tagttactgg actaatttgg
    36901 gcatataata acgttaaatg gtttcatgat ggagtgaatt ctttcttcaa aggtgtttca
    36961 gatgtagctg ttgaaatatt taatttttta agtggttatt ttggtggatt tttcgatggt
    37021 gttatagcaa atttcaataa tttttataat gctggaaaac gaattttcag tggttttcta
    37081 gattttatca ctggagtatt tacagggaat tggtcaaaag catggcaagg tctagttgat
    37141 atttttggag gtattttcga tggtattgtt gctgtaggga aagctcctat taatgccatg
    37201 attggattga tcaatggatt tattggtgga ttgaataata taaagatacc aaaatgggtt
    37261 cctggtgtag gtggaaaatc attttctatt tcaaaactgc cttatttagc acaaggcggt
    37321 catctaatca atggacaagc gattgttggt gaagctggac ctgagttact gactgccaag
    37381 aatggtaaaa ccacagttac tccattatca gacgaagaaa aacgcaaagg gatcagtgga
    37441 agagtttctg gaggtagcat tgaacagcat atacacattg gcaaagtcga tgcaaacaat
    37501 ccatctgagt tagatcgtat gaatcgtaaa tttgctaaag caaacaaaca ggctattcgt
    37561 gatttgggag gtgttcctat ttgagtcgac aatttatgaa tccagatgaa ccaaatttta
    37621 tttggaaaaa tcggaatgca gtaattgata tgaattgcat cattgaggca gagcttccag
    37681 atattatgcc gaacaagcga tacgaaactt acacgattca gggccgaagc ggagaattga
    37741 cggagacgtt tgatgactat gaaccttttg atttggaaat agaagggatc actattccac
    37801 attcgaaact gagagaagtc aaacgatggc tcactggtaa aagccaatta atcacacata
    37861 atgatccaga caagtatcta gaggctatct gcagcatgga taaagaagtc cctttcgaaa
    37921 acgagtgggg cttcttttat acattcgatg ttacttttcg ttgtcagcca ctcaaaagaa
    37981 aaatcggtga agcacctaaa atttttcatt ctagtgttat ggatattttt gatcatggtg
    38041 acgaaattgc acatccctat ctggaaatag aatcgaacgg aggagatata acgatcgctg
    38101 tcggtgataa aagcctaaca atacttaata cgttagctgc aacaatcgtg gttgatactc
    38161 agcttggaaa agcaatacaa gaaggtttga atttatttac gcgtggtgat tggccagtat
    38221 tgcagcctga atggaatcag gtaaaaatat ccggaaaatt taaagaagtg cggttttgga
    38281 ataggagtgt gtatctgtga cgcaagaatt tatttatgct tataaaaaaa tgccagatga
    38341 tttgagcgtg aacggagcgt cgttagttga ttgggaagat ttgccagaaa tcaatcgtgt
    38401 gctgaatggc caatatcgtt tttatggtaa ctattcaaga agcggacagt atcgctctta
    38461 tttaaaaaaa ggaaatttta taaaagcgaa ggtaccagat ggatcatggc aatattttga
    38521 aatttataat gtcaaaaaga atttgacctc cgtttctgta acagctcggc atattggttt
    38581 tatggccaat aagaacttta ttgtcaagtc gtttacggat aacggcaatg ggtctcagat
    38641 catgaccaat cttaaaaaca gtttggcatt tgatcaaaaa tttaactatc tttcgaatgt
    38701 agggacaacg caccaattta cagcaagaca agtcgcgcca gttgaagcaa tcattggttc
    38761 aaataacggt aatcaaaatt tgactggcgt gactagcgcg gaacttgata tggataacta
    38821 tgatttgaaa ctggtaaaac agattggatc agataatggt tttcggattg attttggcat
    38881 caatttagaa gcaatcgaag aagaaattga tgaagaatca attgtcaata gtttgtattt
    38941 ggttggcggt gttcctgata acgattatga tgaagacaaa gaacctatcg aatatggtta
    39001 tttagaaatt gatggagtaa ccaacgagaa tcgacgtatt gccaaacgtg aaaacggaga
    39061 ttgtaaaaca gttgatgaat tgatcaaatg gggcaaaacc ttatttgaca atgatcgtat
    39121 ccatgagcca aaagctactc atacagtcag tatggtggcg ttggaacaca cactcgaata
    39181 cggtgacatg tatcaagagc tagcgtcttt acactttggt gatgtcgctc atgttagggc
    39241 gaaagagcta gatatcgaga taaaagagcg tatggtcgaa tacacttatt tcccaacgct
    39301 tgggaaatat aaggatattg ttttaggtaa tgatttatca ctgtacactt cggcagtaaa
    39361 tactcaagca caagaattga aaaagaaaat cgacaatcga acagaaacgt tagttcaaaa
    39421 tgtgttgaat gcgacggcat ggatcactgg taattctggt gggcatgtcg tttttcgtcc
    39481 agaaaaagct ccatcagaga ttcttatcat ggatactcca aatgtggcga atgcaaagcg
    39541 agtgtggcgt tggaatttaa atggcttagg ttattcagac aacggagtaa atggcccgtt
    39601 cggtattgct atcacatcaa aaggagaaat tgttgctgac ttcatcaaag taggaacaat
    39661 aaatgcagaa gtattcgaaa cttcctttaa tgcttatggt gatgtgttaa aacttgtaaa
    39721 aggcacgctt caaatttgga atgaaaacaa aaaaatcatg gagctaacca aaaaaggtat
    39781 ggaattctgg aatagaaaag aatccattgg aacgatcgga acaacagatt cagcaggaaa
    39841 tccatttcca aatgctgtaa cacctacacc tcttgaggaa aattctttgg taatacgtac
    39901 aaatggcgat ggaaaataca ttttaatttc tccaactgca gaaaaaggat ttgtgttatt
    39961 aggaaacgga aaagcatatt atttcggtga tttagatatt cagggaaaac ttactgttag
    40021 aggtaaagaa gtgataccag gacaaaatgg cggtccatct ggcggaggag aaacacctgg
    40081 tgggtatcct gatgaattaa aaacagatgc agaaaagaga gcttggagaa t
//
LOCUS       DANKBF010000020        38923 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000020 DANKBF010000000
VERSION     DANKBF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(93..650)
                     /gene="frr"
                     /locus_tag="PWY89_001134"
     CDS             complement(93..650)
                     /gene="frr"
                     /locus_tag="PWY89_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2066184.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(653..1375)
                     /locus_tag="PWY89_001135"
     CDS             complement(653..1375)
                     /locus_tag="PWY89_001135"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2066185.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1511..2392)
                     /locus_tag="PWY89_001136"
     CDS             complement(1511..2392)
                     /locus_tag="PWY89_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2066186.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="PWY89_001137"
     CDS             complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="PWY89_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2066187.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3632..4111
                     /locus_tag="PWY89_001138"
     CDS             3632..4111
                     /locus_tag="PWY89_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL2066188.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4328..5419
                     /gene="phnW"
                     /locus_tag="PWY89_001139"
     CDS             4328..5419
                     /gene="phnW"
                     /locus_tag="PWY89_001139"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL2066189.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5412..5540
                     /locus_tag="PWY89_001140"
     CDS             5412..5540
                     /locus_tag="PWY89_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2066190.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5544..6089
                     /locus_tag="PWY89_001141"
     CDS             5544..6089
                     /locus_tag="PWY89_001141"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2066191.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6543..8234)
                     /locus_tag="PWY89_001142"
     CDS             complement(6543..8234)
                     /locus_tag="PWY89_001142"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2066192.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="PWY89_001143"
     CDS             complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="PWY89_001143"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2066193.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9716..10735)
                     /gene="argF"
                     /locus_tag="PWY89_001144"
     CDS             complement(9716..10735)
                     /gene="argF"
                     /locus_tag="PWY89_001144"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2066194.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="PWY89_001145"
     CDS             complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="PWY89_001145"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL2066195.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12516..13217
                     /locus_tag="PWY89_001146"
     CDS             12516..13217
                     /locus_tag="PWY89_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2066196.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13826..14842)
                     /gene="tsaD"
                     /locus_tag="PWY89_001147"
     CDS             complement(13826..14842)
                     /gene="tsaD"
                     /locus_tag="PWY89_001147"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2066197.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14839..15303)
                     /gene="rimI"
                     /locus_tag="PWY89_001148"
     CDS             complement(14839..15303)
                     /gene="rimI"
                     /locus_tag="PWY89_001148"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2066198.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15310..15852)
                     /gene="rimI"
                     /locus_tag="PWY89_001149"
     CDS             complement(15310..15852)
                     /gene="rimI"
                     /locus_tag="PWY89_001149"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2066199.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15836..16558)
                     /gene="tsaB"
                     /locus_tag="PWY89_001150"
     CDS             complement(15836..16558)
                     /gene="tsaB"
                     /locus_tag="PWY89_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2066200.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(16749..17729)
                     /locus_tag="PWY89_001151"
     CDS             complement(16749..17729)
                     /locus_tag="PWY89_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2066201.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17753..19237)
                     /gene="galT"
                     /locus_tag="PWY89_001152"
     CDS             complement(17753..19237)
                     /gene="galT"
                     /locus_tag="PWY89_001152"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2066202.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19249..20238)
                     /gene="galE"
                     /locus_tag="PWY89_001153"
     CDS             complement(19249..20238)
                     /gene="galE"
                     /locus_tag="PWY89_001153"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2066203.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20486..20653
                     /locus_tag="PWY89_001154"
     CDS             20486..20653
                     /locus_tag="PWY89_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL2066204.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20715..22526)
                     /locus_tag="PWY89_001155"
     CDS             complement(20715..22526)
                     /locus_tag="PWY89_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL2066205.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22523..22888)
                     /locus_tag="PWY89_001156"
     CDS             complement(22523..22888)
                     /locus_tag="PWY89_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL2066206.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(23051..23446)
                     /locus_tag="PWY89_001157"
     CDS             complement(23051..23446)
                     /locus_tag="PWY89_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2066207.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(23464..24426)
                     /locus_tag="PWY89_001158"
     CDS             complement(23464..24426)
                     /locus_tag="PWY89_001158"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL2066208.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(24426..24638)
                     /locus_tag="PWY89_001159"
     CDS             complement(24426..24638)
                     /locus_tag="PWY89_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL2066209.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(24659..25357)
                     /locus_tag="PWY89_001160"
     CDS             complement(24659..25357)
                     /locus_tag="PWY89_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2066210.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(25377..25919)
                     /locus_tag="PWY89_001161"
     CDS             complement(25377..25919)
                     /locus_tag="PWY89_001161"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2066211.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(26051..27055)
                     /locus_tag="PWY89_001162"
     CDS             complement(26051..27055)
                     /locus_tag="PWY89_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2066212.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(27052..28041)
                     /locus_tag="PWY89_001163"
     CDS             complement(27052..28041)
                     /locus_tag="PWY89_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2066213.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(28038..28844)
                     /locus_tag="PWY89_001164"
     CDS             complement(28038..28844)
                     /locus_tag="PWY89_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066214.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            29010..29966
                     /locus_tag="PWY89_001165"
     CDS             29010..29966
                     /locus_tag="PWY89_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2066215.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(30043..30561)
                     /locus_tag="PWY89_001166"
     CDS             complement(30043..30561)
                     /locus_tag="PWY89_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2066216.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(30649..30798)
                     /gene="rpmG"
                     /locus_tag="PWY89_001167"
     CDS             complement(30649..30798)
                     /gene="rpmG"
                     /locus_tag="PWY89_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2066217.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            31026..31472
                     /locus_tag="PWY89_001168"
     CDS             31026..31472
                     /locus_tag="PWY89_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2066218.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(31667..33562)
                     /gene="asnB"
                     /locus_tag="PWY89_001169"
     CDS             complement(31667..33562)
                     /gene="asnB"
                     /locus_tag="PWY89_001169"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL2066219.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(33887..34861)
                     /gene="bsh"
                     /locus_tag="PWY89_001170"
     CDS             complement(33887..34861)
                     /gene="bsh"
                     /locus_tag="PWY89_001170"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2066220.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(35195..35282)
                     /locus_tag="PWY89_001171"
     tRNA            complement(35195..35282)
                     /locus_tag="PWY89_001171"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35248..35250),aa:Ser,seq:cga)
     gene            complement(35427..35993)
                     /locus_tag="PWY89_001172"
     CDS             complement(35427..35993)
                     /locus_tag="PWY89_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2066221.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            36249..37580
                     /locus_tag="PWY89_001173"
     CDS             36249..37580
                     /locus_tag="PWY89_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2066222.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            37546..37896
                     /locus_tag="PWY89_001174"
     CDS             37546..37896
                     /locus_tag="PWY89_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066223.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(37988..38407)
                     /locus_tag="PWY89_001175"
                     /pseudo
     CDS             complement(37988..38407)
                     /locus_tag="PWY89_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(38408..38752)
                     /locus_tag="PWY89_001176"
     CDS             complement(38408..38752)
                     /locus_tag="PWY89_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2066224.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
BASE COUNT        11292 a         8078 c         6756 g        12797 t
ORIGIN      
        1 ataatttttt ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg
       61 gaatgatccg cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta
      121 tcagcagcga tcttatctac ttctttgatg ctgttatctg tcaatgtttg tacgtctttt
      181 tctaatccgc gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca
      241 tcgatcgcat cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc
      301 acatctttag ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg
      361 acatttccat cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca
      421 tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg
      481 attgatgcca tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc
      541 aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt
      601 gctgcttttt tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc
      661 cccttacagt tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat
      721 ttaaattgaa gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat
      781 ccatgacttg taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg
      841 catttgcatc tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca
      901 ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt
      961 acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg
     1021 ctttacggcg aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg
     1081 tcggtacacc cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta
     1141 acatacccat gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc
     1201 gccagatatt tccgccgccc acaacgattg ccatttcaac gcccagctcg tgaacttctt
     1261 tgatttcttg gatgatttct ttgattactg gtggtttgat tccaaatcct gcgtccccag
     1321 ctaaagcttc tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca
     1381 ttcctccaat attttctatt gccattttac catacttaga tgatttcgac actctaagac
     1441 tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta
     1501 ttcaacagaa ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt
     1561 tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg
     1621 caacgtattt ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga
     1681 tttctgcttt gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt
     1741 tgccttcgtt caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg
     1801 gaatttgtga ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc
     1861 ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca
     1921 tatgcaagta tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga
     1981 agctgatttt ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt
     2041 tatctgtttt gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt
     2101 ctgcaatttc ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg
     2161 agtttacttc aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag
     2221 ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa
     2281 ctgctttttc catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta
     2341 cgccagtcat ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact
     2401 cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc
     2461 cagcctttga aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac
     2521 aacatctacg atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac
     2581 tacttgatct tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa
     2641 tttaacggca cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc
     2701 gcagtttgta tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc
     2761 aatacgttct ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc
     2821 gataccgcct aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt
     2881 tttaggtaga acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct
     2941 gatacgtttt tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac
     3001 gaagtattgt cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt
     3061 acctacgaat aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc
     3121 atctactaat ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa
     3181 gatatatttc ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc
     3241 ttctagtaat tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt
     3301 tttcctcttc ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca
     3361 ataagaatgt ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc
     3421 cgtatcgcaa gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa
     3481 aaagaattca agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa
     3541 ataattttag gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata
     3601 catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat
     3661 taaacaagtc attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg
     3721 gcattatggc gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat
     3781 cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac
     3841 tcctgagact aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt
     3901 gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt
     3961 atttgcatct gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt
     4021 cgatacgatc atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct
     4081 tcaccatcca actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc
     4141 gtgttttttt attttcaata ttttccttat tgaataaacc tttacactct ttacaaaaat
     4201 acatcatttt ttattcaaat ttttcaacac cttgattgaa tcacaataat catcatttat
     4261 attggatgtt aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg
     4321 aaatcatatg tataaattat taacttctgg accattgacg acttctgaaa cagtgaaaaa
     4381 tgctatgctg atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat
     4441 aagaaaaaaa ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat
     4501 gcaaggtagc agctccttcg gggtagaatc ggtattaagt tctgtgatca ggccagaaga
     4561 tactgtactg atttgttcaa acggcgcata tagacaacga atgatcaaca tgtgcgaatg
     4621 tctgaaactg aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt
     4681 gattacacag ctgattgaag agaatccgtc tatctccgca ctagcaatgg tccatagcga
     4741 gactacttcc ggtattttga ataatttgga agagattgct gctgtcacac aagaaagaga
     4801 tcttttgttt atagtggacg cgatgtcaag ctttggtggg ataccgattt gtgtagccga
     4861 actagggatt gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt
     4921 ttcttttgta cttgctaata aagcggaact ggaaaaacga aaaaatgtta gccgaagttt
     4981 gtcacttgat ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt
     5041 cacttcccct acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga
     5101 aggaggaatc gaaactcgat attcacgtta tctcgccaat aatcatgtgc tacgacaaaa
     5161 aatgacagag atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc
     5221 ttttctttac cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca
     5281 aaatggattt gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa
     5341 tattggcgag atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa
     5401 ggagaacgtt aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg
     5461 gctgtatggc tcctgtccac gcttttagaa atgcttttct agaaaaagga acccaactga
     5521 ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc
     5581 tttccctccc ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa
     5641 aagaggtgga ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc
     5701 attctacctt gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca
     5761 atattggttc cacgaatgga tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa
     5821 aaaaagaaag gtattcacca gacttcatgg caacttttga agacgtcgaa gggtatggtc
     5881 gaccgtttcc ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg
     5941 aagtaatcaa agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt
     6001 tgacagtagg tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa
     6061 aattagagat ggggaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc
     6121 cggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga
     6181 caagaatcca gaatgacttt tatacaaaat aagaggttgt gaaaaagctg tcctgtatca
     6241 agtaataaaa acagccaagc aaaaatagct cctcatattt ttcgtttggt taggcttatt
     6301 accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg
     6361 acttttgtca cagtcccctt atttgcaaac aaacagtgtt taagagacaa cctctccact
     6421 aaacggtgtt cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag
     6481 ctattttctc ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt
     6541 tcttacatct tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca
     6601 gtcactgcat aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc
     6661 gtatgagcat agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact
     6721 gacggttcgt atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta
     6781 accacttccc agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat
     6841 cctgcttttt ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt
     6901 tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt
     6961 ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt
     7021 tccaaatcag ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat
     7081 acttcttcta ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg
     7141 atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt caaagcccat ttccttcaag
     7201 acagctttca actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt
     7261 ttgaagtcgt aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt
     7321 gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg
     7381 atctctgcac ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca
     7441 tccatcttgt cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga
     7501 tcatagattt tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata
     7561 gcctctttat cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct
     7621 ggttctgttt cagctacttc atggaattgg acgtataaac gaagcaattc attgattggt
     7681 tctgctttaa cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac
     7741 tgtgttcccc agtcacctaa atggttgatg cggattggct gataaccgat tttttccata
     7801 atgaaaccga tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg
     7861 gcaatattag gagaagacat gtcgattggt actgttcctt gattcccgat gttgctatcc
     7921 ccataatgtt ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta
     7981 ttcatgaaaa agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg
     8041 attttttctg ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat
     8101 gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgc
     8161 tcgacttgtt ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga
     8221 tctttattat tcatctttat tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat
     8281 ctctagcgac ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact
     8341 ccaattgtcc taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa
     8401 acagaattct ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa
     8461 ttcgctagcc aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac
     8521 tttcttgaat tatatcagaa aaaaaaacga aaggctagtc gtttcataaa aaaaaaatcc
     8581 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8641 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8701 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8761 tagctgcttc tactttcgga agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt
     8821 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8881 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8941 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     9001 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9061 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9121 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9181 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9241 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9301 ccttcagtac ttcgcccatg gcgttttgta accagtaacc gatagatcct tctgtcattg
     9361 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9421 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9481 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9541 ttgcatccgt agacaagatg gcattgcccc ctaaagcgac aactactttt cgtttggcca
     9601 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9661 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9721 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9781 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9841 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9901 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9961 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
    10021 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10081 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10141 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10201 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10261 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10321 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10381 gttcctctac cattttttga ctgaatccac ggaattcgat tccgtcaaac atacgtccta
    10441 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10501 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10561 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10621 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10681 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10741 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10801 agtaagactg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10861 tgcagcgtgg accaccacga cctcgtgaca attcacttga attgatttct aacactttga
    10921 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10981 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    11041 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11101 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11161 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11221 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgttcgga attttgattg
    11281 ccaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11341 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11401 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11461 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11521 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11581 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11641 catatagact gctagaacgt acttcgatat cttttgtacg aaccccagcc atgattttat
    11701 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11761 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11821 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11881 atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11941 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccgtttca
    12001 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12061 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12121 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    12181 acgtttttct atttttaagc gtaatttatt cattttttat tctgtataca ctttcaaaaa
    12241 aatatttatt cattgaaaaa atttttttga ataaattaaa aaaataaatt atattatcgt
    12301 tgttagcgtt tactatatgt attattgttg ttttttatac acaaatcatt cgcttcaaaa
    12361 caaattattc aataccattg tatatttttt acataataac aaattatatt tgtaaagtat
    12421 taaaacttgt ttctaatgtt ggtatgttaa aactttacaa attctttaaa aaaagtgaca
    12481 ataaatatag tgaacaataa ggagaggact ttttaatgcg tgttaataaa atcgattttc
    12541 acttttcaaa actcagatat caacctgatt ttgaacattt ctcggatgaa gagttccaac
    12601 agatcaagga acatactgtt cttcgttctt ataaaaaagg acagatcctt ttcgacgatg
    12661 gtgatcccag acaacggttg tatatcttga ccaaaggact cgttcgttta gaacgttatg
    12721 atgaaagtgc aacttattac tactatgact atgtgaatac aaataacctt tttcctatgt
    12781 caggaatgtt tggagaagag acctacactt atacagctca agcgatgact gatattgaaa
    12841 cattctacct tcctgtttcc tactatgaaa aattagcacg aaaaaatccc gaacagttga
    12901 tctatatgat acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt atccaaatcg
    12961 gattgacctc tagcgccttt gaccgggtaa aacaaaattt attcattttg aaagaagaat
    13021 tgggcgtacc aacaaaagat ggtcatatca ccattcctta tccgatcacc ttaaaagaat
    13081 tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa caactacgtg
    13141 attccgggct gttagattat gacaaaaagc gttttacttt ctgtaagaca gctactactc
    13201 cagctatgag agaataacgc gaacaaaaat aagcagtgac cctgataaat atcagggtca
    13261 ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc actggacaag
    13321 actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc aaaaaatact
    13381 gttagattaa ctagatgaag cgtcttgtcc tcatccgatc tagattattc gatattcctc
    13441 gacaataaac gattactcag tgagggcaat cagaggaacg gataatgata aataagctgt
    13501 aaaaaatagc aaaataacaa taatgattaa aagtatgtaa aaaatagggc tttgacaaaa
    13561 acgtgtcaca gccctatttc gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt
    13621 cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga atcagagtta
    13681 aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata cgcggtaata
    13741 agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt attacttgat
    13801 gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga tcagcattca
    13861 actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct ccaatcattg
    13921 cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca gggagtttag
    13981 ttgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca cctccagcta
    14041 taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta atcaatacat
    14101 cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct tctccacgct
    14161 gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa ctgaaatcat
    14221 aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc tgatgagcta
    14281 gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca actttgtcat
    14341 aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat ccatcttctt
    14401 gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat ggaaactgga
    14461 atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca ggaataagcg
    14521 gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc aacgagccga
    14581 caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac acaccagctt
    14641 ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg ctagctactt
    14701 caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg atgttagata
    14761 agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt gattcaatcg
    14821 ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc tcataatgat
    14881 cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac gaaacttttc
    14941 tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa aaacatgcag
    15001 cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc
    15061 ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta ctttcgagta
    15121 tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag agtgaggctg
    15181 ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat aagcattttc
    15241 gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt ttctgcaaat
    15301 caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt cttttgtttc
    15361 agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat aaagtctttg
    15421 tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat tcattatagc
    15481 aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat atgccggtcg
    15541 aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga taaaaccaat
    15601 cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact tcaattctga
    15661 tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt cgacatccag
    15721 cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat cgatttgttc
    15781 gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat acgctctaaa
    15841 ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt tcttctgctt
    15901 ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt tcaactaaag
    15961 cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact gtattaatca
    16021 tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca ctcccgacga
    16081 aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta
    16141 catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca gcgtatacat
    16201 tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg caattagccg
    16261 cgatggtttt cagactagat actcctacta gctctttatc taatgtataa gcaatcgttt
    16321 ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca gaaaccgcga
    16381 ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg attgcaggca
    16441 tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt
    16501 cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat gtaatcatat
    16561 atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt tttattctga
    16621 ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc tggcgggcaa
    16681 attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc ccacagcttt
    16741 tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat aatctttctt
    16801 gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag ctctcccatc
    16861 cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc tacactgaca
    16921 tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc ttgaatcgct
    16981 ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga
    17041 ttcgttttta aatactgctt cactgcttga tatcctgact cgacagaaaa atctgcttcg
    17101 ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc cataattctg
    17161 ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag catagcgata
    17221 tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga cttaaagtcg
    17281 acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt
    17341 tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc gatcgttcca
    17401 tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac gtttgcttct
    17461 tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc aagctcttcc
    17521 gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc atttttacga
    17581 tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt ggctaagcca
    17641 acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga aactcctgcc
    17701 aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac gtttataaaa
    17761 tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat actccagcgt
    17821 cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg gtgaccgtat
    17881 ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga tgatattcag
    17941 ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct ggtttcaaac
    18001 gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc tttttgatat
    18061 gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa acattgttgt
    18121 ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg
    18181 tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca tccgaatatg
    18241 ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc tctgttgatt
    18301 gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc ggataatctt
    18361 ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga cgtcctgcct
    18421 gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca ttcgaaccaa
    18481 caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga
    18541 ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa taagcataag
    18601 gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc cggtgattgg
    18661 tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa agcatgcagg
    18721 taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg
    18781 gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc ggaaatttga
    18841 tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa ttaaagaaat
    18901 actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca ttgacgactg
    18961 aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt tctgctggca
    19021 attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat aagtctacag
    19081 attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct accatcccta
    19141 aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct tcgatcgcaa
    19201 gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct attcttcata
    19261 accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt tgatatctgt
    19321 gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga ctaatcggct
    19381 aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg tcacttctct
    19441 agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat tgaagaagtt
    19501 gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa tcaaatcttc
    19561 tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt ctccataaac
    19621 agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa gatgcgtttc
    19681 tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat tgaagtagcg
    19741 caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt tttccatcat
    19801 caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg tgattggaga
    19861 ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat tcacgttgaa
    19921 ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga aatacatcaa
    19981 tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta ctccttcaat
    20041 cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc cttcataaaa
    20101 atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta ccacagctac
    20161 ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac cggctccgcc
    20221 tagtactaaa attgtcataa aaaacactcc tttgattagt aaaattgtgt ttgttgattc
    20281 tatataattt aaaaaacgaa gtttactttt atggtgaaaa caaacaaagt aaaatttcgt
    20341 aaattcatat ggtaaattta taataaaaca tttagtaaac attttcaagg attttttgca
    20401 aaggtttaca aacaaacgta aaactagcct ttttttctct tttgttccat aatagatata
    20461 acaaataata aatgaggtgg attcaatgaa aaaatttgta tctggaatat tagtaggaag
    20521 tctggcaaca gccgctgccg ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga
    20581 tccaattgac gaaaaagaag caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg
    20641 cgtttctcgt taataattat ttaaaaatac gcatgaagac tttctaaaaa acagataagt
    20701 catcatgcgt atttttattt gtcagtcaat ataagttgtg ctattttatc tgcctgtctc
    20761 agattccatg gatctgtata aggtctagct actgctgcag ttagccaatc cacaccttca
    20821 acaggtattc catagcaata aaagtcagac tggatttccc ctgaagcatc aatcacgcga
    20881 ttcgtacctc gctcaacatc tatagcttct gaatgataac ttcctgtatt atctgaaaat
    20941 gaaaaagggc gaacaagttt gttttccttc aaatttttta tgacaggatt acttgaacga
    21001 gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc gactgctgat ttctttatct
    21061 tgtagagaaa taacatagcg gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa
    21121 tgaaaaatac ccgcatctat cagtgctgct aattctttcg tccgaatgat tggcggaccg
    21181 atcgttaaaa agctatttag atgagtaaac catccccata gtatttcttt gtattcctta
    21241 gcagaaaaaa cgtgccattc aatagcctgc cggattggat cacgccagtc ttttaatgca
    21301 tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct
    21361 tcaataaata ttcggctagc ttctgaaaag ggctcattct gataggcaag aggagtttct
    21421 aaatacgacc aatcccatct ataagggatt agtgctggat gctttccgag acactcctct
    21481 gaagagaggg agagaaaatc atgctcaaaa tttttctttg gaatcggcaa gtgatattct
    21541 tcgatgattt tcttgtaata aaacaactca acgtcttttt tcagatagtc aaaaaatgtt
    21601 tcgccagtca aaaatcccga ctgatgccat tgctccaggt tttcatttgt tagtaaccgt
    21661 ggccatgcca ttgctccgcc ttgcttttga tttcttcctc gagggtaata agggagcccc
    21721 cttcttgatc cactgtatac aatcggttcc ttgcctgatg gatgatagac cagttttccc
    21781 ttttcgttct cgaagcgccc tcctcttgat actgtaaaca atccgacata atcaaaaaaa
    21841 cttaagccta gacctcttaa aaagacattt tctttaggcg gtatcttcga aagatctaca
    21901 tctgcaggat tagaaggaag ctgatacagt aaaacatttt tctttgcgta gtctacccac
    21961 actctttctt cttctgtctc atcattttcc cagtgaccgc ttgctaaaac aagctgatcg
    22021 atagaatagc tttgctgatc tgtctgtaaa agataaccat cttttctttt gcttactttt
    22081 tttactaaag aataaacaaa ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa
    22141 aaccagcgtt gatagagtcc gtataaacat cgagtagatt gctgatcttt ttccagacga
    22201 ttgatttctt ctataattag ttgcttattt ttggttaccg tctgttcgat ataatctgta
    22261 gcaggtcctt tactccattg aaacaagttg ggtccctgtg ttaccaatcc tccactggac
    22321 aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag aattcattaa taactccggt
    22381 ggctgattca accgccaaac tcgacctcca ggaccattgg gatcgaaaag aaaaagttca
    22441 agagaatgtc tgtcctgatt attatggaga atcctttctg ctacacttaa tcctctaggt
    22501 ccggcaccaa tgatccctat tttcatttgc atgcctccat ttcgataaat tttttcaata
    22561 aaacagcatg gttctgatgt tcattttttg caccataaac aaaagtaatg actggatgtt
    22621 ctttcataag ttgactgagt ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt
    22681 cttgctggta tttctttgca aatgaagcaa atcgttctgg tacatggtca aaagttttgc
    22741 gtgcttcatt actaggtgca atttctttca accacagatc taactgcgct ttttcttttg
    22801 aaattcctcg tggccagaat ctatccacga gtattctata gccatcctct ttttcagcat
    22861 caaggtaaac acgttttaat ttgatcatag ttgtttcctt ctttctacct tttcgcttag
    22921 cttatatatg tagataatag cacaaaataa aaaaaatgaa cggcacaact atccattgcg
    22981 ccgttcatgt gatcttggtc aaaatctaat gattgattcg gtttttaaag ctgtcataca
    23041 atcatctgtt ttatactttt ttacttttga tttttccaga ccatccgtca tatccaccct
    23101 taagtaaata aatctcttga taacctgctt tacgtaattt atttgctgta cggatgctca
    23161 aacttttctt ttgatcgtaa atatagacag gctggtcttt tcgaatagat cctaatgaat
    23221 ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat atgcccagca tcaaacacat
    23281 ctttttcgcg aacatcgatt acttgtgctt ttctcattcc ttctcgaaat tcttcttctg
    23341 tcaaagtcgt tgctgaacgt ttggccatta ctcgtagata gacttcccaa aggatgattg
    23401 caagcacaat caatagcaaa atgatattaa ttacccataa aaccatgtaa tgcctccgtt
    23461 tttttaagca aatttcaagg ctaatgaggc agcaccaatg acaccagctt cattaccaag
    23521 ctctgctaat ttgatttttg ttgattctct tacttgcggg aaagtaaatt ctttaaaata
    23581 tttctctaca cggctacgta aaaattctcc tgctgccgat actcctccac ctaacacgat
    23641 acttgacgga tttaatgtat tccccaagtt tccgcaagct aaacctaagt agaagcatac
    23701 gcgatcaaca accatcaatg caaacggatc gtcttcttga gccatttcaa agatatcttt
    23761 acttgttact tcttctccgt tgtctagcat tgctttcagt tttgaatccc ctgcgtattc
    23821 ttctgccaag aaacgaccta ggcgtaccac acctgtagcg ctagatacag tttctaaaca
    23881 tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc acagtgatat gtccgatttc
    23941 tcctccagct ccgctcacgc cgtgaatcaa atttccgcca gcaacgatac ctccgccaac
    24001 acctgttcct agagtaacga atacaacgtc tggatcgttt tctccagcac ctttccatcg
    24061 ttcacctaaa gcagcaacat tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc
    24121 ctcgatcagt tttttcacaa attgaacttc tgtccagttt aagttatacg caccaataac
    24181 cgttccagct tcgctgtcca cgcttcctgg ggtacccatg ccgattccga taaagtcttc
    24241 ggctttcata ttatataaat tcaaacgatg attgattgat tcaatgattt caggaacgat
    24301 atgcttacca tcatccaaaa tattggtatc gatgctccat ttttgctgaa tctctccttc
    24361 tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa tcaatcccaa taatcttctt
    24421 gtccattaca aatttccttt ctttcggctt tcttcaattc ggtgctctct ttttaatatg
    24481 cgccacgctg tcatgtatgt atcatgatca atcaaacggc tatcatacag attacgcaat
    24541 tcgatagtca ttaattcaat gtcatagatg cgtgcaccca catagataaa gatgccaaat
    24601 tttttgaaca gctgctgtac atcatataag ctctccatct ttaagcactt ccttcatatt
    24661 atacaagtaa accatatttt tttaaaccca gtaaaataca tattacaaaa agaaatacaa
    24721 aaactagtgt agaaagaatc ctttggtgta tcgaatagct tgtctgttgt ttcggtagac
    24781 ccaaaacatt ccctaacact aatccgccaa ttagcccacc gacatgtccc caaacatcta
    24841 cagaggtatc cagaagacca aaaacaaatg tcaacacaat aaataaaaga aaatgtctga
    24901 ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc taagataaac aacgcaccga
    24961 acaaaccgaa tatcgaagta cttgcaccac cagacagaac attcgcttcg ttaaaagcaa
    25021 agcttgctgt atttcccatt atcccgctga acatataaat caagaaaaaa cgccaatggc
    25081 catagattgc ctcgatttgc tgccccataa aatacagtat cagtgagttc attacgaaat
    25141 gcatcagtcc aaaatggatg aagattggtg tcactaaacg ccaccattca ttgaagtgaa
    25201 caagataagg accatacatt ccggcataat aagggatctc aagtcgcgga aataattcca
    25261 tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata ggtccataac ggtcgtctta
    25321 accatacacg caattttatt tgctgttgat aattcatgaa aacctcccat ttcattttag
    25381 tcaataaaca attgttggat cttctgatca aagctctccg gttgccattg ttcattcaat
    25441 tgttcacgaa aaacaaggct acatgttttt ttatgaaaat tatttaaata ccggtcgtaa
    25501 taaccaccgc caaaaccgat tcgataaccg gctctattgt agataatacc tggtacaatg
    25561 aggagatcga tcatctccgg caaatagagc ttctctgatt gtggctcaag aacaccaaaa
    25621 ctcgaacgta caagaacgga ctgttcatcc atttcataaa acgccatctg acgtttcggc
    25681 aaagttttag gtacaactat tttcttttgt tctttttctg ctcgttggat aaccggctgt
    25741 gtatccagtt caaattcatt cgataaagtc gtccctatca cttttgctga cttccagtac
    25801 tcgctggcga atagctgttt atagattcgc tgttcttttt cctttttctt gccacttttg
    25861 gccagatttt tcagcttttc gattcctttc cggcgtaatc ttactttcaa ttggtccaaa
    25921 aactcaccct tccttctctc ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac
    25981 tttcaaataa atgcataaat agaataaaaa gaagacctag aaatgattgg ccttcctttt
    26041 ttaatgaatt tcaactgttc agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat
    26101 atggcgcacc aaaaagggca atcaaaattc ctgtaggtac ttccccactt agtagaatca
    26161 ctctagcaac catatctgca ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa
    26221 aggtccagtt attttcttta cgtacgacta gcttcgcagc atgaggagct agtagtccta
    26281 caaaagataa actacctgtc accgctacac taccgcttgc caaagcaaca gctaacaaca
    26341 aaaggatttt ttgactttta gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa
    26401 atgatagcaa tacaatcctc tgtgcataaa gtcctcctag cggaattaag ataaccgccc
    26461 atggaagcaa tgcccaaaca taaggccagc tcgcacccca gattgatccc gctaaccatg
    26521 cattcacaaa tccataattt tccttagata attggatcgt gcatagcaat gttaatgcag
    26581 aaaggccggc attaacggca ataccggaca ataagattcg attcataccc aaaaagccat
    26641 ttcgatcata ggccgcaaga tagacacagc aagcagctaa aacaccacct agaaaggcta
    26701 caactagcaa tagaaacggc agcgaatagt gcgaaaagaa acctaaataa agcatcacta
    26761 aaaatcctgc tccggcatta atgcccaata tactagaatc tgccaattca tttcttgtta
    26821 ctccttgcaa gatataacca gaaacgccaa gacaagctcc tgctaataaa ctgatcagta
    26881 atcgaggaac tcgaaattct ctaacaatca gcgccaaaga agatgattct ttccctatga
    26941 acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc aatcattaaa ctacctatag
    27001 acaggatgac aagtacaaaa agcaagaaat agaacgtcca atatattttt ttcaccgttg
    27061 ttccctcctt acttgtatca gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa
    27121 aggtgtttca aaagggggat tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc
    27181 accgccaact aaaaaagaga caggtagaac ataacgatag tccgttccaa ccattccgcg
    27241 aacaactggc ggaaccaaca gcccaataaa tgaaacaggt ccgactaacg aaacggcaat
    27301 ggctgctaac actgcaacta gacagaaaac aatcaaccgc gttcgtttag gatgacttcc
    27361 aagagctaat gcatgtgttt cactcaaatg aatcaaagat attgcttttc ctaatgaaaa
    27421 agaaaccaaa acagcaagta tatataaagg aaacaatact gctaattgtt tccatgtgat
    27481 attggcagct ccgcctacaa accaaaaagc aagatcctgc tgcagattaa agaattgggt
    27541 aagtgcttgg ctgatagcag taaaaaaaga actaatagct acgccagcta aaatcagctg
    27601 taaaggattc atgcccattt tcgaaaactg caaaaaacca aatacgagca aagttacagc
    27661 aaaagaacct gccaatgaca ttagaaacac agaaaaatca gaagctttag gccaaaaagc
    27721 aaaactaagg gccattgcca acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc
    27781 taaaggattt cttgtcactc cttgcatcaa tgcaccgctc aatgcaaaac aactccctac
    27841 aagaaatgct cccgccacac gaggcaaacg aatatttcgt atgatttggt gactttgttt
    27901 tgatctatca aaatcaaaca acgcctgcca aaaatctgct tttcccacag tcgctgcgcc
    27961 atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct
    28021 atttttcttc atagtcctca taattgatcc tcctgctctt ttggcaacaa atcataggtt
    28081 agaaaaactg gtttatttgt tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt
    28141 tttttcatac actcttttgt gatcacttct tcaggtgtac cttctgtgat caacttaccg
    28201 tttttcattg ctaataaata atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac
    28261 atcaagatcg ttgtttgttt catttgattg atttcagtca ataactgtag gatctctaat
    28321 tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga tcaagatatc tgtttcctga
    28381 gcaagcgcca tagcgatcca gacacgctgg gtttgtccac cagacagcgt tgcgatatcc
    28441 cgcatacgaa atccagctag acctgtagca tccaacgccc actcgattgc ttcatgatct
    28501 tgcgtgtcca tgccactgaa cagtttttga taagggtatc gtccgtaaga tactacttct
    28561 tctactgtca tacctgtagg gtgttcacta gtttgtgcta ataatgcaat tttttttgca
    28621 agttcttttg aactgtatgt agagatatca gagttattga caatgatctt tcctgatttc
    28681 acaggtagta tacgggtaaa acttttcaat aaagtagatt ttccacttcc atttggccca
    28741 atcaaactag tgattttccc ttcaggaatc gtaacagaca tgttttcaat gatttttttg
    28801 ttttcgtagc cggctgcaat gtcagctgct cttaatctcg tcatttcacc catcctttcg
    28861 ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc gtcaagcgct actttcatat
    28921 ctatagccaa atgaagcggt tgacagcgtt cgaaatatca ggaataataa ccaatgagaa
    28981 agattctcaa ttatgttagg aggaaagaaa tgaaattaaa aacaaaagta ttcacattat
    29041 ctagcatatt gctgctggct ggagtactga gcgcttgctc cccctcttca gataaagaaa
    29101 cagctacaac tgccaattcg acgacttcat ccaatagtac tcatacagtc acagatacac
    29161 ttggacataa agtagagatt ccaaacgcgc ccaaacggat catcggcagt tacttagaag
    29221 actatctgat tgcactaggg gaaaaaccta tcgcccagtg gactgttggc agtggttcca
    29281 ttcaagatta tctgcaagat gatttaaagg atgtcccaac gatttcttat gaccttcctt
    29341 atgagaaagt gttaagcttt gagcctgatt tgctgttgat ttcttcttcc gctactgtcg
    29401 aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa
    29461 cagatgtcac atgggaagaa caactaaaag atgtcggcaa agctttggca aaagaaaaag
    29521 aggcagaaaa gatcatttcc gattatcaaa aatccatcaa gaaaactcga gaagaactag
    29581 cagataaaat cgagaacaag acagctgcag tgttgtgggt gaccaacaac tcagccttta
    29641 tggtcagcaa aaatcggtca agtggaagaa tcgtatacga cgatctggaa tttggtgttc
    29701 ctgatctagt ggaagaggtc tccaaaaaag ccacttctga ctggtctgct gtttcctctg
    29761 aaaagctagc tgaattagat gctgactaca tcatccttgt gaacagtgac aaagatgctg
    29821 ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt gacagacaat catgtactgc
    29881 aattcggacc tgaatctagt tggctatata acggacctat tgccagtcag gaaatggtca
    29941 atgatatcaa agaaaaacta aattgatttt aactaaaaaa agcatcttca cgattagtcg
    30001 tgatatgctt tttttcttat taacaaacta gacgcataga ttttattctt cttttgaagc
    30061 aaccctattt tttactcttt cccatttttg aaaacgatgg gcatatagat tttcttgatc
    30121 cgttatcccc tcatactcct ctataagggc gaagtctgaa aaatcaatat tcccaatata
    30181 cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag atccgaccag tgtaaggaag
    30241 aagttcttga aaaatccgac taccacccga aacatagata tcttctgaga tcgtttgact
    30301 atacgacaaa agttcatcga tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt
    30361 tggctgcgtt gtcaacacga tgatatggcg ataaggaagt gaaagatccc ccattccttc
    30421 ataggtcttt cttcccatca ctaaaattcg atccattgta tgttcgcgaa agaaacgcat
    30481 atcatttggc aatcgccatg gtaacaaacc atttttgccg atcagaccat ttttatcttg
    30541 tgcccacatg gaaataaaca tattatccgc tccttatgat tttagatgtt ttgagataaa
    30601 aaaagtctca attctttgac cagcaaggaa ttgagaactt taaaaacatt attttgtttc
    30661 acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg
    30721 attgttacgt ttgtttttgc ttgttagata gttacgttct ttacaagaag tacactctaa
    30781 agtgatgttt acgcgcatgt ttttccctcc catttttaca agaatcctct aagatgactc
    30841 tcagacttga ttatcttacc atgatttttt ctagattgct agtgtttttc acgattttgt
    30901 taagtaaaac tacttgacga taaatacaac ggttttactt ctcaaacaaa atctttttct
    30961 ttttgtataa atacccttac acttgtttat atagattatg ttttttaata aagggggata
    31021 ctaatatgcc atggaacatg aaggactttc ctgcatcaat gaaaaactta gataaattga
    31081 caagaaaaaa agcaatcgac atcgcaaatg cattattaga tgaagggtac cctgataacc
    31141 gtgcgatccc aattgccatt gatcaagcaa aaaaatggta tgaagatgct agtgaatcag
    31201 aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac ggatgaacac gatacgaatc
    31261 ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga atatgaggaa gaacaatgga
    31321 tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt tgatacaaaa aaagaagcaa
    31381 tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac tttgttcatt tataaaaaag
    31441 atgggacaaa agaaaaagaa atcgattatt gaacatatgt aaaggggttg tgacgaaaat
    31501 cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct ctgccaactc cgaaccataa
    31561 gtcataatat ctataaaacg ggaagagctg ttttttgaaa ttgtttctta tcactcagtg
    31621 ttaaacggta ggagaaaacc tcttttttat tgatatttga ttttcatcaa acagtcacat
    31681 agtcgattgc ttctgcttgc ggaatcgttt catctacaaa gtagacttta taccatgtca
    31741 agaaactgaa gattgtccag atttttcttt gctcttcttt acgtccttcg tgatggtcat
    31801 ctagcaactt catgattttt tcttgatcaa aaaattcttt tgcaaaatct gcttcgaaca
    31861 atgctttgac ttgttcgtag ccgtgttctt ctttcaacca agctttgatt ggcacaggga
    31921 atcctaattt cacacgattc gaccattttt ccggaagatg tttgttcgca gcttgtctga
    31981 aaatatcttt tgtgttgttt tgattcaata aaaacttact tggtatttgt tgagccaatt
    32041 tcatgacttc gatatccaat aaaggtactc ggacttccaa agaacttgcc atagaaagtt
    32101 tatctgcctt taacaagata tcttttggca tccattgatg aagatcgaca tattgcatct
    32161 tattgacttc atctttgatt ccttctgttt tctcgtaatg aacagtcatg atttcatcca
    32221 cagatggtgc tgtttgatat tttggtgtta acacttctga tgcttctgat tcttcgaaca
    32281 ctcgagcatg accgataaaa tattcttttg caggagcaag tgattcatat aaatggatcc
    32341 gtccatggaa atttttcatt tttccgattt tacgtcctaa attatagcgt gttccttttg
    32401 gtaatttttt caatccttga gcaatcacac ggatgaattt tgaattcgta tggaatccat
    32461 acgtttggta tcccgcaaac agttcatccg ccccttctcc agattggact acccgcacac
    32521 tttgtgaagc aagatttgct aaaaagtaca aaggtacgca agaaggattt gaatcaggtt
    32581 catctaagtg atactggatc aaagggaatg ctttgaacga catgtctccg tcgatcactg
    32641 ctgctgtatt atctaagtcc aatagttcgg ttagttttct ggcttcaatc gcttcattat
    32701 aggttttatc atcaaacccg attgagaaag aatgatctgg tttcaataca gaggttacat
    32761 aactggaatc aaccccgctg gataagaaag aaccaacttc tacatcactg accgtatgtg
    32821 cttcaataga agcttgaacg gtttcgtcga ttttttcgat ccattcttgt cgggaatgcg
    32881 tttcttttgt ttcaaaatct gcatcccaat attgctgaat gtctattttt ccatctttat
    32941 acgtataata atggccttca ggcaaacgat agacatcttt gaagaatgtt tcgccgttta
    33001 atggagaata ttggaaagtc atataaggtt tcaacgcttc tttgttcaat tctttgttaa
    33061 aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa cataaatgtt ccgttcattt
    33121 ctgcataata atatggtttg atcccaaagt ggtctcttgc accgaataac tcattctttt
    33181 cattatccca aatagcaaaa gcaaacattc cgcggatctt ttgcaataat tctgttcccc
    33241 attcttcgta accatgcaaa aggacttctg tatctgcatg tgtttgaaaa acgtggccag
    33301 ctgcaatcaa ttcttctctc aaaggctggt aattatagat ttcgccatta aaaataatga
    33361 tctttgtccg atcttcattg taaataggct gagtaccgcc ctctaagtca ataatactta
    33421 atcttctgaa tcctaaagca acatgtttat cgatatattc tcctgaacta ttaggtccac
    33481 ggtgaacgat tcgatccatc attgtattaa taattgtttt tttgttgtcc ttgtcgttaa
    33541 caaaacctac aataccgcac atttaacttt cacatcctaa tataaaatta ctgtttattt
    33601 cattcatatg catcttatca tagaataaat agcggacgaa tattttctct cttacaattt
    33661 cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca gaaaataaag tcccaatatc
    33721 taatttacca tgttttcttt tttgaggcta ccttattctc ttcgtgttaa aaaaatatta
    33781 gtggatgcta tttccaaaaa aaccagcaaa aaaagacacg acctaaagat cgtgtctaat
    33841 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
    33901 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
    33961 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
    34021 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttctttct ccagtatcgc
    34081 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
    34141 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
    34201 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
    34261 tgatctggtt tgaaaaatga ttttctggtg ttccatttga tagcgagcga taattgttca
    34321 gattaaataa ttgataatca aatggcggat tatttgtcaa cacaccgaca ggattatcat
    34381 ataaatgaag tccatctttc atactttcaa ttactatcga tttttcttta tcagctaata
    34441 accagtgtaa aggtgacaag ggtagttttt cactaaaatc gatattgact agattgatag
    34501 tagccaataa ttctttagct tcgtctatgg ttgagcattg ccctagtatc catggaataa
    34561 actcaaatgg tgcaacgtta tcttttcctt cttgtaattc tttataatca gcattgccag
    34621 gaaaattcag tcctgccata ctcaaccctt tttcatttgt agcctcataa tagagtggat
    34681 agttggatac cccagctgct attcctatca ttgcataatg tttatctaaa ttttttacct
    34741 ttctaaaatc gaaacgataa tttcttggag taatagtgac cacttcatta taagacatct
    34801 cataatcaaa attccttcca aaataatggt ctttcgttgc ataagtgatt gcggtacaca
    34861 tcgtttcccc tcctaaaaca aatcgttttt acacttttat cttacatcta gtattttttt
    34921 tatcaaaaaa aggccaaaga agattgattt actagcattt ataaaaaata gtactcaggg
    34981 ggaatcaaaa aatattgtag gttaaaagta caacaataag gggctgtgac aaaagtcttg
    35041 tcacagcccc ttattccgaa caaacggtgt tcaaaagctt gcatctgcgg taataagccc
    35101 aatcaaacga aaaaatagga aaagctattt ttgtttggcc gttcttatta cttgatgcag
    35161 gacagcttct tcacaacctc taatctttga tataaggaag gtacaggatt tgaacctgcg
    35221 cgccggtatt agccggttcg ccggatttcg agtccggtgc attaccactc tgccaacctt
    35281 ccgtaagtac tatttttaaa cttttttcac gaaatgtcaa gcctatttct aatagataaa
    35341 taacagaaat cgcttttttt agcaaaaaag tccattgaca gtttgcagcc aacggactta
    35401 ccgatcaaat tcatactgat agaacactat ctgattattt caggattatc taatgctaaa
    35461 atcgagtcaa ttgtccgttt gatcagtgca atgatttgtt cttctgttgg atgcatgatg
    35521 tttgatgaca tcaatgacgc taagccagtc actgctaccc atgtaccagt atgcagagca
    35581 tcgacatatt catccgataa ttgattgtat ctttcatctt ctttaactat ttcacggaaa
    35641 taattgtatg aaaaatcatg cattttcttt ccgtccccat gttcatcaat gtacaatgcc
    35701 atgtaaagac tatgatgctt ttttgcaaag tagatatagt tcaaaccgag atcaatgatt
    35761 ttatcaccag tacgttcaat agggaaaaca tcttctttta gttgttcaaa gatagaatcc
    35821 agcaacgtat tttttaaatc ttccatgttt ttgaattcca gataaatcgg ctgtgtcgaa
    35881 atccccatct ttttagcaat atttctcgca gtaaatcctt taaacccttc ctttgcaaca
    35941 acatcataag cagcttttag aatttgatcc ttggtataga cttttcttct cattaaaact
    36001 cccccacttc tttactgcag tatatgttgt tttattgtac cctattttta gcattttttg
    36061 caaacatttt actcaaaacg ttatatttta aatttttttt cagtcttctt taactttttt
    36121 ttgcaaaatt aaaaaaattt ggttaaattc aatgggttcc cttacatttt tagttttgac
    36181 ctaaatgata gttgtccaaa tctttcattt caagttatag ttaaagaaaa tgaaaaagaa
    36241 ggtgtgtaat ggaaaacgaa actgacgtta tttatattca tcctcaaaaa cgaatcgttt
    36301 cacagaaaag aaagtatttt tatcttggtt ttacaggtgt tttttttctt tttattggct
    36361 tgctttctaa tactcctact gataattgga gtggattgct aactattctt acctcgccaa
    36421 gcaatctttt gacagattat tttgctttag gcggtttcgg aagcgcattc ataaatgtag
    36481 ggattttgac cttactttct gtgcttttag cttatcggca caaggtgatt ttaaacggtc
    36541 ccttatttgc ttcgattcta acagttaccg gtttctcttt ttttggcaaa aacctttata
    36601 attctatctc aatcatttta ggtgtgtatc tatacgctgt atttgtcaat aaaccttttt
    36661 ctcagtacat catgatcgga ctgtttggta gcgcattatc accggtcgtt agttatatca
    36721 cttttggtat gcgttttcct ttactgatag gtattttatt ggggaattta gctggcatag
    36781 caattggtct acttcttccg cccttagctg ctcaaacttt agttttccat cgtggattca
    36841 cactttataa tattggattc acctctgggt tgattgcgat gacttttact gctgttttga
    36901 ggttattcag ctattcaatc gtcgaaaata cattggtttt caatgaatat cactttcctc
    36961 tcatttggat tatttttggc tttttttcgc tgacagtcgg aattggtttc tattataatt
    37021 cctttcggct tttggggatt cgagaaattt tcgattcatc tggaaaattg acaactgatt
    37081 tcattgctaa ttctggtatt ggtgctactt tgatcaacat aggcttagtt ggtctgatgc
    37141 tgtcttctta tgttttgctg gttggcggtc agttaaatgg acctgtcatc ggtgctattt
    37201 tatctgcggt gggtttcagt gcttttggtt gtcatttaaa aaacagtttt ccgatactag
    37261 tcggaatatt tattgcttct ttgtttggaa cttttcatga gatcaccagt actggcatgc
    37321 ttgttgcagc tgtgtttggg acaggtctcg cgcccataag cggtttttac ggcagttttt
    37381 acggtgtaat agctggtgta ctacatattg ctcttgtcca taacgtcagc actttgcacg
    37441 gaggactaaa tctatataac agtggttttt caactggttt cgttgcaggt atccttgtac
    37501 cgatactaga taactttacc gctgtcagaa aggaaaaaaa gacacttgga aaaagaatta
    37561 tcaaaaagaa tcatcgatga gcttcttact tatttatatt cccatgaaat aactgaaata
    37621 cgtattggag tagaatttgc tgaggaaggg cttttcatag agatacaggg tacggcagaa
    37681 actcagccag aagatttaga tgtacttgct gatttgctga acaatcctcg ggaccctagt
    37741 atggaaatct attatcaaga actattaggt aatccccgac atgatcggga agattatcat
    37801 ctgttaggta gtttgattga tgacgtggaa atcatgtatg cccagccgat tatcaatatt
    37861 aagatctttc gacgtgcttc tcattcctat tattaaccct attttctatt tataaaagac
    37921 tgaatcaaaa aagcagctcc tgaaagttcg attaaaatct actttcagga gctgttcaag
    37981 cttgacatta ttgattttcc aatccttcat acacttcatc tgttacaggt tctaaaaact
    38041 ctgaattacc agcagtgatc gccaaatgtt caaaccaact gtcttttgtc gcaccatgcc
    38101 aatgtttaat ccctttgtgc gttacgacga catctccagc cattaatcgt cttgctggct
    38161 ttccttcttc ctgataccag ccttctcctc cggtcacaag taagatttga tagccgtctt
    38221 tatggacatg ccagtgattg cgacaacctg gttcaaaagt tacattacct acaccaacag
    38281 aaacttcggg atcattcact aacatattca gatagcttta cccggtaaat acttgagcgt
    38341 aagcttcgtt tttttcacct gctggaaaaa tcacgccatt gtgtatttct tcgtgttttg
    38401 tcgtcattta cgcctctttc ttatcttcgc tattttcttc taaatagatt tcttttgcta
    38461 aactaaaagc tgaccaagct tttggccatc ctacataaaa agctaaatgg gtaatgattt
    38521 ctgctatttc ttcactagta atgccatttt gcttgccaat cttcagatga gctggcaatt
    38581 gttcaaaatt tcctcctgaa attaatgcag atatggtgat gatactacgc tgatgagcag
    38641 ataattcttc tctggaccat acttgtccaa ataaaacatc atcattcaat tctgcaaatt
    38701 ttggtgcaaa atctcctaat tgatctcgtc ctgcggtttg tttttctgcc atttctctta
    38761 ttctcccttc acgactgtta ggtgattcaa ctatggttct aaggcaactt tattttctat
    38821 tgtttcttcg catttcattt taaatgtagt ctttcagcct gtccaatgct tagtttattc
    38881 agtcgatatg ctttttaagc atagttgcta acgctttttt tat
//
LOCUS       DANKBF010000021        36731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000021 DANKBF010000000
VERSION     DANKBF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(154..552)
                     /locus_tag="PWY89_001177"
     CDS             complement(154..552)
                     /locus_tag="PWY89_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL2066225.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="PWY89_001178"
     CDS             complement(549..1322)
                     /locus_tag="PWY89_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2066226.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="PWY89_001179"
     CDS             complement(1319..2002)
                     /locus_tag="PWY89_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2066227.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="PWY89_001180"
     CDS             complement(2169..3056)
                     /locus_tag="PWY89_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2066228.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="PWY89_001181"
     CDS             complement(3053..3922)
                     /locus_tag="PWY89_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2066229.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="PWY89_001182"
     CDS             complement(4059..5345)
                     /locus_tag="PWY89_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2066230.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="PWY89_001183"
     CDS             5618..6196
                     /locus_tag="PWY89_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2066231.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="PWY89_001184"
     CDS             6196..7125
                     /locus_tag="PWY89_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2066232.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="PWY89_001185"
     CDS             7122..8858
                     /locus_tag="PWY89_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2066233.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="PWY89_001186"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="PWY89_001186"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL2066234.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="PWY89_001187"
     CDS             complement(9754..10263)
                     /locus_tag="PWY89_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL2066235.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="PWY89_001188"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="PWY89_001188"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2066236.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="PWY89_001189"
     CDS             complement(11564..12655)
                     /locus_tag="PWY89_001189"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL2066237.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="PWY89_001190"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="PWY89_001190"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2066238.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="PWY89_001191"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="PWY89_001191"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2066239.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="PWY89_001192"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="PWY89_001192"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL2066240.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16618..17592)
                     /locus_tag="PWY89_001193"
     CDS             complement(16618..17592)
                     /locus_tag="PWY89_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL2066241.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17573..19456)
                     /locus_tag="PWY89_001194"
     CDS             complement(17573..19456)
                     /locus_tag="PWY89_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL2066242.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19474..21699)
                     /locus_tag="PWY89_001195"
     CDS             complement(19474..21699)
                     /locus_tag="PWY89_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2066243.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21814..22848
                     /locus_tag="PWY89_001196"
     CDS             21814..22848
                     /locus_tag="PWY89_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2066244.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22915..23928)
                     /locus_tag="PWY89_001197"
     CDS             complement(22915..23928)
                     /locus_tag="PWY89_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2066245.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24006..24194)
                     /locus_tag="PWY89_001198"
     CDS             complement(24006..24194)
                     /locus_tag="PWY89_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2066246.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24634..24762)
                     /locus_tag="PWY89_001199"
     CDS             complement(24634..24762)
                     /locus_tag="PWY89_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2066247.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24765..26138)
                     /gene="rlmD"
                     /locus_tag="PWY89_001200"
     CDS             complement(24765..26138)
                     /gene="rlmD"
                     /locus_tag="PWY89_001200"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2066248.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26249..27304)
                     /locus_tag="PWY89_001201"
     CDS             complement(26249..27304)
                     /locus_tag="PWY89_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2066249.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27314..28744)
                     /gene="gatB"
                     /locus_tag="PWY89_001202"
     CDS             complement(27314..28744)
                     /gene="gatB"
                     /locus_tag="PWY89_001202"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL2066250.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28741..30213)
                     /gene="gatA"
                     /locus_tag="PWY89_001203"
     CDS             complement(28741..30213)
                     /gene="gatA"
                     /locus_tag="PWY89_001203"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2066251.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30213..30518)
                     /gene="gatC"
                     /locus_tag="PWY89_001204"
     CDS             complement(30213..30518)
                     /gene="gatC"
                     /locus_tag="PWY89_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL2066252.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30624..32660)
                     /gene="ligA"
                     /locus_tag="PWY89_001205"
     CDS             complement(30624..32660)
                     /gene="ligA"
                     /locus_tag="PWY89_001205"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2066253.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32677..34911)
                     /gene="pcrA"
                     /locus_tag="PWY89_001206"
     CDS             complement(32677..34911)
                     /gene="pcrA"
                     /locus_tag="PWY89_001206"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL2066254.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35066..36625
                     /locus_tag="PWY89_001207"
     CDS             35066..36625
                     /locus_tag="PWY89_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL2066255.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10133 a         7980 c         6638 g        11980 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    16561 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    16621 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    16681 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    16741 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    16801 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    16861 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    16921 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    16981 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    17041 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    17101 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    17161 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    17221 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    17281 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    17341 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    17401 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    17461 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    17521 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    17581 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    17641 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    17701 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    17761 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    17821 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    17881 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    17941 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    18001 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    18061 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    18121 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    18181 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    18241 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    18301 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    18361 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    18421 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    18481 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    18541 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    18601 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    18661 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    18721 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    18781 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    18841 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    18901 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    18961 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    19021 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    19081 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    19141 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    19201 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    19261 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    19321 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    19381 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    19441 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    19501 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    19561 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    19621 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    19681 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    19741 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    19801 gtcttttact tgcttttctt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    19861 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    19921 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    19981 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    20041 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    20101 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    20161 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    20221 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    20281 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    20341 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    20401 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    20461 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    20521 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    20581 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    20641 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    20701 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    20761 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    20821 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    20881 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    20941 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    21001 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    21061 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    21121 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    21181 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    21241 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    21301 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    21361 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    21421 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    21481 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    21541 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    21601 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    21661 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    21721 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    21781 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    21841 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    21901 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    21961 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    22021 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    22081 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    22141 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    22201 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    22261 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    22321 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    22381 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    22441 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    22501 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    22561 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    22621 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    22681 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    22741 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    22801 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    22861 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    22921 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    22981 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    23041 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    23101 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    23161 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    23221 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    23281 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
    23341 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    23401 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    23461 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    23521 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    23581 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    23641 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    23701 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    23761 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    23821 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    23881 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    23941 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    24001 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    24061 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    24121 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    24181 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    24241 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    24301 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    24361 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    24421 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    24481 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    24541 cttattggca aatcgccaaa ataatgtaga ctaactaaaa agactaagaa acaataacaa
    24601 aagttaacac ttcttagtct ttctttagca cgtctattta ttaaagattt tagggccttt
    24661 tctcatgttt ttacgatcat tcttaggtgt ttccatttgt ttggatcggc ctccaccttg
    24721 tttctttttg atcaattctt tcattttttc tttttggtcc atttttattc tcctttaacg
    24781 aatgaagcga ctaattcgat atgatgggtt tgcgggaaaa gatctacagg ctgaactttc
    24841 ttcacggcat aacctgcttc tgcaaataac ttagcatctc ttgcaaaagt tgccggatta
    24901 catgagatat agacgattcg ttctggtgac atttcgattg ctgattcaat aaaatgatga
    24961 tctaatcctt ttcgtggagg gtcaactacg atcacagatg gtttgatccc tttttcttgc
    25021 cattttttca taactttttc tgcttttccg acttcataat gaacattttc gattttatta
    25081 tttaaagcat tgaactgtgc atcttcaatt gcttctggaa taacttccat cccatacacc
    25141 ttatccactc tgtctgctaa agacaagcca atcgtaccta ttcctgaata agcgtcgatc
    25201 acaatgtctt ttttatctaa ctcggcaaac tcgatggctg tttggtacag atactctgct
    25261 tgttgagtat ttacttgata aaatgacttt gctgagattc gataggtttt tcctaatagc
    25321 tgatcttcga tatagtctct gccatataaa actttttctt tgtctcctaa aatcacattt
    25381 gttttttctt cattgatatt ttggatgacc gaaaccactt ctggcaattc ttcatgaata
    25441 gcttcagcaa tttccttgga tttaaagaat ttttctttac gagtaactaa aacgaccatc
    25501 atttcatggg aataatgacc acgacggata atgatgtgac gaagctgtcc ttcatttttt
    25561 tcttcattat atcctcgtac ttggaaccgt tgcaagatat ctctgactac tttgattgct
    25621 tgatcgatga caggatcttg gatataaaaa tcttcgatag ggactagatc atggctattc
    25681 tttttgtaaa aaccggtggc taactgtcca tcgatacggc gaacagggat ctgtgcttta
    25741 ttacggtagc cgaatggttc tgccattccg accgttggaa gtacaggaac ttctggcatc
    25801 ttagcaatct tcgccattac atgctctact tgtttttgtt tgaagacaag ctgttgttca
    25861 tacttcaaat gggctaaagg tgcgatacct atgcgaagta atgcattgtt ttctacaggc
    25921 tgacgatcag gtgattgtgt aataaatgtt tcgacttttc catatccaaa cttattgcct
    25981 gcttttacta ctagtatttc tacttcttct ccagggagtg cattttcgat aaataatgga
    26041 taaccatcga tttttgctac ccccattcct tcataactca agtcgacgat ttcgactgta
    26101 tatcgttcgt ttttctttac tatttgctgt tctgtcatat tttgctcctt taagcctatt
    26161 tttcaatcct actgaactct actataaatc agctaattac acaactaaaa ccggcactga
    26221 ccttactacg ttcagcaccg gcttttgatt aattcttttg attaggtgtt tcgtctgcta
    26281 tttttccgtc accgtcgata tcttcatccg ttaaacgttc gacttctttt acgaattctt
    26341 tgctgacaac ctctaattct tcttcatctg tacctgtgat tgcttcatca ggaatctgat
    26401 cggtattggc aaagaactca atgtgttgat gaagattttg gaaatgcatc ggtgcttccc
    26461 cgccgtattc accatctaag ttgatcatta ctggtcgatt gatatcatct tctgtttcaa
    26521 cgaacaaact gcttgttttg gtatagatta ttcttggatc atcgacatgt cgaccgccat
    26581 tcagcatgag tgctgcaata tgcaagatct caaagatatt tgctgtcttc acaatgatta
    26641 aagaaaactt cccatcatcc aatttggcat ctggcgcgat ctgttcgaat cctccaacag
    26701 agttcgtcag ccctaggaaa aacatcgaag catttccgat atattcccct tcatcatact
    26761 tcaagcgcat tttgattggc tttactcgag ggagcatctc tgctcctttc gcaagataag
    26821 caagataacc aaaaatactc tttagttctg atggaacttc ataagtcaat tctgtcaaat
    26881 agccacctgc agcgatattg atgaagtaac tgttatcaga aaggccgatg tccattttta
    26941 cggtttggtg tttttttatc acttctgctg ctgctttaat attatctcta ggaattttta
    27001 atgctcgagc atagtcgttc gttgtaccag ctgggatgat ggccatttta ggacggtgtt
    27061 caagcggagc gattccatta accacttcgt tgattgtgcc atcgccacca gctgccacta
    27121 ctagatcaaa acctagttct gcgactctac atgcttcatt cttggctgaa tctggttctg
    27181 gtgtagttgc ataggcgctg gcttcaaatc ctgcttcttc taatgcctgt aggatatccg
    27241 ccaaattttt tttcaataat tcttttcccg aggtggggtt ataaattacg cgtgcttttt
    27301 tcattttcag cctctatcgt ttcgctaatt catctttaag tagcttattc acgacaccag
    27361 gatttgcttg tcctttagtt gctttcatga tttgaccgac taggaagcca accgcacgat
    27421 cttttccatt tttgaaatca tcgacagatt gttggttgtt atccaaaatt tcattaatga
    27481 ttggcagtag tttgttcgga tcagataatt gcacccaacc atttttttca actacttctt
    27541 gggcttctcc gccatttgtg atcaattcac ggaagacttt tttggcgatt tttgagctga
    27601 tcgttccatc ggcgatcaaa cgaatcattc ctgctaagtt ctcaggtgtc aattttgtct
    27661 cagctaattc cagtttctca ctgttcaagt aagcagaaac ttcacccatc aaccagttag
    27721 atgcttgctt ggcatctgca ccattttcca aaacagcttc aaaaaaatca gacatttctt
    27781 ttgtaagtgt caataccatt gcgtcatact ctggaagtcc aaactcacgg atatagcgtt
    27841 cgcgacgaga agctggcatc tcaggcaaac tagcttttac tttttgaatc cattcgtcat
    27901 cgatcacgaa tttcgggata tcaggctctg ggaagtaacg ataatcacta gacccttctt
    27961 tgacacgcat caaaactgtt ttattcgttg tttcgtcaaa acggcgtgtt tcttgttgga
    28021 tttctcctcc tgaaagaaga acttttgctt gacgtttttc ttcaaaagct aaaccttttt
    28081 tcacgaaaga taaagagttc aagtttttca attctgcttt cgtaccgaac tcttcttgtc
    28141 cataagggcg aagagaaata ttggcatcac agcgcataga gccttcttcc attttcacat
    28201 cgctgacttc tgtaaattgg ataattgatc gcaatgcttc tagataagcg tatgcttctt
    28261 ctggtgaacg catatcagct tcagaaacga tttcgatcaa aggcgtgcct tgtcggttca
    28321 aatcaacgta agaataaccg ccaataccat gcatgttttt tccagcatct tcttctaaat
    28381 ggacacgttc gatccggatt tttttctttt taccttctac ttcgatttcg atccatccgt
    28441 catgaccgat tggttgatca aattgggaaa tttgatatgc ttttggatta tctgggtaaa
    28501 agtaattttt ccggtcaaaa tgagtatctt tagagatttc acagtttaat gccaaagctg
    28561 ctttcatccc gaattccagc gcagctttat tcattactgg cagcacgcct ggataacccc
    28621 agtcgatcac gtttgtattg ctgtttggtt ctgcaccaaa atgagctggc gctggagaga
    28681 aaatttttga gttggttttt aactcgacgt ggacttctag tccaatgact gtttcaaaat
    28741 tcattcgtct ttccctccta aaatagctgg ttgttgttta tggaattctg ttgcttgttc
    28801 aaacgcatat gccgcttgat acatcgtttt ttcatcaaaa taattaccga tgatctgcaa
    28861 tccaactggt aatccttctg ataaacctgc cggtacagac atacctggaa gtccagccaa
    28921 gttgacaggg atcgttaaga tgtcactcat gtacattgtg attggatcgt taatgttctc
    28981 accgattccg aatgcaactg ttggtgaaga tggcccgatg atcaagtcat aatctgcaaa
    29041 gactttttca aaatctcgtt tgatcaatgt acggacttgt cctgcttttt tgaagtaagc
    29101 gtcataataa cccgcgctta aggagaatgt acctaacatg atacggcgtt ttacttcttc
    29161 accgaatcct tctgaacgtg agttgacata tacatcatct aatgatttca cattttcaga
    29221 gcgataaccg tagcggatac catcgaatcg ttgcaaattg gaacttgctt ctgatgaggc
    29281 aatgatataa taaacagcca caccgtattt tgaatgaggc aagctgactt cttccacaac
    29341 agcacctaat gaacggaatg tttctgctgc tttcaataca gcttctttta cgccaggggc
    29401 cacaccttcg cctaagtatt ctttaggtaa agcaatcttc attcctttga tgtcgcctgt
    29461 cagtccctct gcaaaatcag gaactgaaac gcctgaagaa gtaccgtctt tttcgtcata
    29521 accgctgata gctgtcaatg ctaatgcatt atctttcacc gtacgggtca ttggtccgat
    29581 ttggtcaaga gatgaagcaa aggcaatcaa accaaaacgt gagacacggc cataagtagg
    29641 tttcatccct acgatgccgt taaaagctgc tggttggcgg atacttcccc cagtatcact
    29701 acctaaagaa actggaattt gtccagctgc aactgctgct gcagaaccac cagaagagcc
    29761 accaggtact tttgttgggt cccaagcatt ttttgttttc ttgaaataag aagtttctgt
    29821 acttccaccc atagcaaatt catccatgtt tagcttacca acagggatca tatctgcttg
    29881 atagatttta tccatgaccg ttgcatcata aattggttca aaattgtaaa ggatcttaga
    29941 tgcagcggtt gtcaagatat cttttgttac gatattatcc ttgataccaa ttggaatacc
    30001 agcaaggacg ttgctttcat caatcccttt ttcatctaaa gcttttgcct gttccaatgc
    30061 tttttcttcg ttcaatgtaa tgaatgcctc gacctcaggc tctgtttctt tgattcgttc
    30121 aaatgttgtt tttgtcaaat cttgtacagt gatttctttc gatacaagca tatgatgcag
    30181 ttcttctaga gaacgattgt gtaattctgt cattatgcgc cagcctcccc attatcgatg
    30241 attgcgggta ctttgataaa gccgtcttct gtttccggca catttttcaa taattcatcg
    30301 cggttccatc cagcttgcgc ccgatcttgt cgcatcacgt ttactgtttc aaccacattc
    30361 gacgtaaatg gaacaccttc cgtatctact tcttccagca tttcaaccat atcgatgatt
    30421 ttacctaatt ggtcggtaaa acttgcaagt tcttcatctg cgaacgcaag ttttgctaat
    30481 ttggcaacgt gtttcacttg ttcttcacta atcgccatac taccccttct ttctaaacga
    30541 gttaaaaact ttgctctcta ttttaacaca agagtgccgt gtctgaaatt gtttattgaa
    30601 atttgtctga aaaaactcat ttttcattct tctactgagc gacttttagc caatgcatct
    30661 gccatttgat cttctgtcca aacttcgatt cctaattctt gtgctttggt cagcttgctg
    30721 ccagcatctt ctcctgctac cacgatatcc gtttttttcg atacgctacc agtcactttc
    30781 ccgccaagat tttcgatcgt ctcttttgct tcttcacgag taaatcgtgt tagtttacca
    30841 gtaaggacta ctgttttttc tttaaaagga gactcgactt cttgcaattg ggcatttcga
    30901 agccctttgt attcgaaatt gacgcctgct tgttttaatt cattcatcaa ttcatggact
    30961 tcttcgttac tgaaataagt gactacactg tcggcaatga tatcaccgat cgtatcaagc
    31021 gcaataatcg actcatagtc acttttactc aatgtttcta gatcaccgaa atgttctgct
    31081 aaaatttttg ctgctttcgc tccgacatgt cggattccta acccgaaaat caaccgctcg
    31141 accgaattat ctttgctgtt gtcgattgct gtcaaaatat tatttgctga tttttctttt
    31201 attttgtcca acgtcaataa ctcttcttct gtcaatttgt aaagatcagc aacatctgct
    31261 actagttttt tatcatacat ctgttctagt acgcgcggcc ccaacccatc gatattcatc
    31321 gcatttcttg aaacaaagtg gttcaaacct tctttcattt gagcagggca tttaggattg
    31381 atgcaccgta aggctacttc ttcatccaaa tggacaagtt cacttccaca aactggacaa
    31441 tgtgtaggca attggtattc ttcgctgtct ttaggtcgtt tatctaacac gacttgcgaa
    31501 acttctggga tgatgtctcc cgctttataa atcagaactg tatctttcaa gcggatatcc
    31561 ttcaacttaa tataatcacc gttatgcagg ctggcacggc ttactgtcgt tcctgctact
    31621 cggactggtg tcatgatagc tgttggcgtt actacccctg ttcgaccgac tgtccattcg
    31681 atgttttcga ttagcgtttc aacttcttct ggcggaaact tgtaagctgc tgcccatctc
    31741 ggtgctttga tagtaaaacc aagttgatct tgtagcgaga attcattcac tttgatgacg
    31801 attccatcga tttcataggg caaatccaca cgtttgtcat gatattcttc aatgtatgac
    31861 cacacttcat caatcgaatg acatagacgt ttttcccgat tcgtgtggaa tccaattttt
    31921 tctagctctt ctagcgcatc atactgggtt tttgcttgca taggaccgaa atcagctact
    31981 gtgtataaaa aagtgtccag atttcttttc gctgtgattt tagaatctaa ctgacgcaaa
    32041 gaaccagctg cggcgttccg aggattcgca aagatatctt ttccctctgc ttctctgtct
    32101 tggttcaatt ggacaaatga ccgttttggc atgaaacatt cgcctcttac ttcgatgttc
    32161 attggttctt ttaatttgat tgggaccgac cggacagttt ttaggttttc agtgatgttt
    32221 tctcctacag ttccatcccc gcgagtcgca cctctcacga aatttccatc ttcataacgc
    32281 aaagaaacgg aaagtccatc tatttttagt tcacagcaat agtcaactac acgtcccact
    32341 gctttttgta cacgttgatc aaaagctatc agctcttctt tgctgaaaac gtcgttcaaa
    32401 ctgtaaagcg gaatatcatg agtgactttt tcaaaacctt ctaatacttt tccgcctact
    32461 cgttgagtgg gagaatcaga cgtgatcaaa tcaggatatt gttcctcgat tgctactaat
    32521 tctgcgtatt ctttatcata aacatagtct tccactgttg gtttatcttc tacgtagtat
    32581 tcgcgagacc attggttcag tcttgtacga agttcttctg ctcgtttagc agcttcgtca
    32641 atcgtcattt catgttccat catttaccct ccactactaa gctttttcaa ttggcgcaaa
    32701 ggcagccaaa agacgtttta ctccttgact tgggaaagcc acatctagtt ctagatcctt
    32761 cgacgtcccg cttactcgga cgactgttcc tagaccccat tttttgtgtt tgactttatc
    32821 tccaggtttc cagctgttat tttcccctcc agtagcactt ttagagggaa caaccgctgt
    32881 ctctggctgt ttatagctat atgctgtttt tttagcgaat acaggcgttt ttttaggcgt
    32941 aggacgcatg ccttcgattt ccagtaactc ctgatcgatt tcttctacaa aacgagaagg
    33001 tcggttatat tgcgtccgtc catataatgt tctggagaaa gcatttgtta aatacaacgc
    33061 ttcttctgct cgcgtgatcc ccacatatgc gagacggcgt tcttcctcca gttcgctttc
    33121 ttccatcaat gctcgagata aaggaaaaac cccctcttct agaccaatca ggaatacaac
    33181 tggaaattct aatcctttgg ctgcatgcaa agtcatcaat gtcacttggg aagcatcttc
    33241 ttctaaattg tcaatgtcgg aaacaagtgc cagatcattt aagaagaccg ttagtttttc
    33301 ttctggtgca tcagcgtctt cttcattttg ctgttcgaat tgtttgtcga attcttgtgt
    33361 gaccgttaaa aattcctcca agttttcgat gcgtgcttgt gcttctaatg tattttggat
    33421 ttttaagtct tctagatatc cgctacgatc caaaacctct ttcgtcaatt ctgtcactgt
    33481 caagtatggg atcatctgag tgacttcttg gatcatctct ccaaatgcac ctaattgctg
    33541 tcctgctttt ccaccgatat ttgccagatc cacattttgc gctgcttcaa gcagtggcca
    33601 ttcatgaaga gaagcaaaac tgcgtagttt ttcgatagat cctggtccga tgcctcgttt
    33661 gggcgaatta acgatacgct caaaactgat tgaatcttga ggatttacca gcacattcaa
    33721 atacgctaaa atatctttga tttctttacg atcgtagaac ttgtgccctc caaccatttt
    33781 ataaggaata tttgctttca acagagtttc ttccattaca cgagattgtg cgttcgtccg
    33841 gtacaagatg gcaaagtcgc cgtaattccg atgattgctt cgaatctctt cttgcatccg
    33901 gtctacgata aaacgggttt cgtctcgttc attatctgca cggtagtaag tgatcttgtt
    33961 tccttctttg ttttctgtcc acaagttttt tggttttcga ttgctattgt tttcaatgac
    34021 ttggtttgct gcgctaagga tgtttttcgt ggagcgatag ttttgttcta gcaaaattac
    34081 tgcagcatct ggataatctt tttcaaaatc caagatgttc tgcatatctg caccgcgcca
    34141 gccgtaaata ctttgatctg catctcccac gacgcacagg ttcctaaatc tacctgccaa
    34201 taaattgact agcgtatact gtgcgtgatt tgtatcttgg tactcatcta catgaatata
    34261 atggaatttg ttttgataat acgtgagtga atcaggatgt tcttcaaata atcgaatcgt
    34321 attcatgatc aggtcgtcaa agtccataca ttgattatat tgaagctctt tttggtaagc
    34381 agcataacat tttgccgcaa tctcttcgta ataactgcct tgcatttttg caaaatcctg
    34441 aggggtctgc aggctgtttt ttgcttgaga gattgttcct aatatacttc gaggatcata
    34501 ttttttcgga tcaatgttca gttcttttag tatccgtttc atcaaggtca gctgttcaga
    34561 agaatcgatg attgtaaagt tccggttata cccgataaaa tcaacatctc ttcttaaaat
    34621 acgcacacac atcgagtgaa aagtcgatac ccagacatct tcgcctccag atgcgagaat
    34681 cgcattgaca cgttctttca tttcttttgc agccttattg gtaaaagtaa tcgctaggat
    34741 gttccaagga ttgacttctt tttcctcaat caaataggca atgcggtgtg tcagcactct
    34801 cgtctttcca cttcctgcac ctgccattag cagtaatggt ccgtcagtgt gaagaacagc
    34861 ttctttctga cgggggttca tcccgtttaa taattcagct ttggatgaca ttaacaacat
    34921 cctttctttt atcaatcttg ttaattatag cattttcttg gctttcatta aactagctag
    34981 ttcaaaatca tttgtttctt tttatggatt tttccttgtc aattcgttga tttcgcgcta
    35041 aattaaaaga aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga aattagataa
    35101 gactcagata tattttattc taaaaggaat atttgttgga ctgctgacag gaatcgtcgt
    35161 tagcctgttt cgattatcta tcgaaaagct aagcgattta gttcgaagta tctatgaaat
    35221 gagtcgggaa cagccgattt acttaattgg cctagctatt tgctgcatcc ttgcagcatt
    35281 tttcgtgggt tatctggtca aaaatgaacc ggatatcaag ggaagcggga ttccacaagt
    35341 agtgggacag cttcgcgggg aattatcaat gaactggttc tctgttttat ggaaaaagtt
    35401 cattggcggt gtactttcag ttggtgcggg cctttttcta ggtagagaag gaccttctat
    35461 acagcttggc gcatccattg gacaaggagc tggacaattg tttcggagtc catcttcaga
    35521 agaaaagatt ttaatttcta gcggtgcaag tagcggactt gcagcagcat tcaatgctcc
    35581 tatcgcagga ttactctttg ttttagaaga aatccatcac tccttttctc cacttgtttg
    35641 gctgacatca tttgcttcag caatcactgc taacttcgtc tctctttact ttttcggctt
    35701 gagacctgtt ttatatctag gtgaccttcc ttctttgccg ttgaaatatt atggttcctt
    35761 agtgatctta ggagtacttc taggcatctt aggttttatt tatcagaatg tcttgctggc
    35821 tttgccaaaa ttttacaacc gattgccttt accagcttat tttcacgggt tcgttccttt
    35881 cttgctgatt ttgccaattg gttatttatg gcctagtact ctaggcggag gcaatcaaat
    35941 cgttttagct tttggacatg cttccttgcc aatcattatg attatcggtt tgtttgttct
    36001 tcgattcgtt ttttccatga tttcttacgg tgggggtctt ccagggggga ttttccttcc
    36061 gatattgaca ttgggggctt taatcggtag cttttatgga aacattatgg ttgatttcgg
    36121 gatggatcca atctacatca aggattttgt cgttattgcc atggctggtt atttcacagc
    36181 gattggaaaa gcaccgctga cagcaattat tttagtgaca gaaatggttg gctctcttaa
    36241 tcatctgatg cctttaggat tggctgctct tgttgcttat atcgtcaatg accttctcgg
    36301 cggtaatcca atttacgaat cattattgga acgtctactt ggaaatactc agaccgcctc
    36361 actaaaaggt gcaaggacaa cttttgattt tccagtaacg gcagagagtt ctttagacgg
    36421 attaatggta cgagacttca attggcctaa aaatatgctg ctaatctcta tacgcagagg
    36481 aaatcaggaa atcttgactc acggtgatac gatcatgaat gtaggagata ttttggttat
    36541 cttgactgat gaagcaaact tgccaagaat caagcaggaa atcgaggcta aatcttctat
    36601 cacaaatttg aagcaatcat tttaacgaac atggaaaaac tattttacaa caactattca
    36661 tctacactac ttgattctat atgattctat aaaaaaagaa gaggctggga aaacccagcc
    36721 tcttcttttt t
//
LOCUS       DANKBF010000022        36690 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000022 DANKBF010000000
VERSION     DANKBF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            69..3347
                     /locus_tag="PWY89_001208"
     CDS             69..3347
                     /locus_tag="PWY89_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL2066256.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3888..5795)
                     /locus_tag="PWY89_001209"
     CDS             complement(3888..5795)
                     /locus_tag="PWY89_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL2066257.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5925..6800
                     /locus_tag="PWY89_001210"
     CDS             5925..6800
                     /locus_tag="PWY89_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL2066258.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6880..7854
                     /locus_tag="PWY89_001211"
     CDS             6880..7854
                     /locus_tag="PWY89_001211"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2066259.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8034..9179
                     /locus_tag="PWY89_001212"
     CDS             8034..9179
                     /locus_tag="PWY89_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2066260.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9316..9660
                     /locus_tag="PWY89_001213"
     CDS             9316..9660
                     /locus_tag="PWY89_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL2066261.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9931..11217
                     /gene="efmA"
                     /locus_tag="PWY89_001214"
     CDS             9931..11217
                     /gene="efmA"
                     /locus_tag="PWY89_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL2066262.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11355..11954)
                     /locus_tag="PWY89_001215"
     CDS             complement(11355..11954)
                     /locus_tag="PWY89_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL2066263.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12358..13479
                     /gene="mnmA"
                     /locus_tag="PWY89_001216"
     CDS             12358..13479
                     /gene="mnmA"
                     /locus_tag="PWY89_001216"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2066264.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13725..15146
                     /gene="arcD"
                     /locus_tag="PWY89_001217"
     CDS             13725..15146
                     /gene="arcD"
                     /locus_tag="PWY89_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL2066265.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15160..16278
                     /locus_tag="PWY89_001218"
     CDS             15160..16278
                     /locus_tag="PWY89_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL2066266.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16338..17630)
                     /locus_tag="PWY89_001219"
     CDS             complement(16338..17630)
                     /locus_tag="PWY89_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2066267.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17762..18340
                     /locus_tag="PWY89_001220"
     CDS             17762..18340
                     /locus_tag="PWY89_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2066268.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18469..18846
                     /locus_tag="PWY89_001221"
     CDS             18469..18846
                     /locus_tag="PWY89_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL2066269.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18779..20851
                     /locus_tag="PWY89_001222"
     CDS             18779..20851
                     /locus_tag="PWY89_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2066270.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21062..22009)
                     /locus_tag="PWY89_001223"
     CDS             complement(21062..22009)
                     /locus_tag="PWY89_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2066271.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22255..22719
                     /locus_tag="PWY89_001224"
     CDS             22255..22719
                     /locus_tag="PWY89_001224"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2066272.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22781..23824)
                     /locus_tag="PWY89_001225"
     CDS             complement(22781..23824)
                     /locus_tag="PWY89_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL2066273.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24201..25166)
                     /locus_tag="PWY89_001226"
     CDS             complement(24201..25166)
                     /locus_tag="PWY89_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2066274.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25400..26245
                     /locus_tag="PWY89_001227"
     CDS             25400..26245
                     /locus_tag="PWY89_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2066275.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26436..27623
                     /locus_tag="PWY89_001228"
     CDS             26436..27623
                     /locus_tag="PWY89_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2066276.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27629..27985
                     /locus_tag="PWY89_001229"
     CDS             27629..27985
                     /locus_tag="PWY89_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL2066277.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27987..28325
                     /locus_tag="PWY89_001230"
     CDS             27987..28325
                     /locus_tag="PWY89_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL2066278.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28401..28657
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28720..29754
                     /locus_tag="PWY89_001231"
     CDS             28720..29754
                     /locus_tag="PWY89_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2066279.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29747..30433
                     /locus_tag="PWY89_001232"
     CDS             29747..30433
                     /locus_tag="PWY89_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2066280.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30457..31305
                     /locus_tag="PWY89_001233"
     CDS             30457..31305
                     /locus_tag="PWY89_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2066281.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31502..32275
                     /gene="sufC"
                     /locus_tag="PWY89_001234"
     CDS             31502..32275
                     /gene="sufC"
                     /locus_tag="PWY89_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL2066282.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32288..33574
                     /gene="sufD"
                     /locus_tag="PWY89_001235"
     CDS             32288..33574
                     /gene="sufD"
                     /locus_tag="PWY89_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL2066283.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33571..34806
                     /locus_tag="PWY89_001236"
     CDS             33571..34806
                     /locus_tag="PWY89_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2066284.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34793..35263
                     /locus_tag="PWY89_001237"
     CDS             34793..35263
                     /locus_tag="PWY89_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL2066285.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35268..36659
                     /gene="sufB"
                     /locus_tag="PWY89_001238"
     CDS             35268..36659
                     /gene="sufB"
                     /locus_tag="PWY89_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL2066286.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        11994 a         6781 c         7867 g        10048 t
ORIGIN      
        1 tgacagtaaa aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag
       61 gaatacactt gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc
      121 tcagtacccc ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga
      181 acgaagaaaa tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa
      241 ctagtgaaga tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg
      301 aaaagacaaa aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag
      361 aaagcacaag tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt
      421 ccacgaataa cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc
      481 aagcaatccc caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga
      541 cctatacgaa tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc
      601 atttgtttgg gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat
      661 atgtctggga ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc
      721 gacttggatt ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc
      781 cagctcctgg gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg
      841 aaaaagaaac aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca
      901 cgaactcgat tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat
      961 attattacga tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa
     1021 aattatacgc ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga
     1081 cgatcaaggt agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc
     1141 aagaaagacc tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag
     1201 gaaaccaaga tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa
     1261 ataaagcaac aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg
     1321 aatttgatta tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc
     1381 cagataaaaa ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga
     1441 ttcttcatac agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag
     1501 ggtcagatgc aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat
     1561 acgcatatgc tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat
     1621 tgacgattcc aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga
     1681 aaaatccaga aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac
     1741 tgttcagtca ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa
     1801 aaggattaca gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg
     1861 gaaatacagg aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc
     1921 ataacttagt caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg
     1981 atggatttgc ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt
     2041 atcataatgc agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa
     2101 aagtaatcat caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag
     2161 aaggtaacgg taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga
     2221 tcaatagttt gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata
     2281 ttatcgtcaa tagcagtaca agttttaata acggttcata taatgtggct ctttatacct
     2341 cttcagcagg aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc
     2401 ttgagatgag agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt
     2461 tgaactacta ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat
     2521 caaaagattg gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc
     2581 gctatgaaaa tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc
     2641 aaacagaaaa ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag
     2701 aaacctcacc gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa
     2761 aaccaaatga acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg
     2821 cagaaggaaa tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa
     2881 atcaaggaac gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg
     2941 aaacacagtc aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag
     3001 gagaatatac gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg
     3061 ctcatacaga atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc
     3121 ccccgggaaa tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac
     3181 caaatggaaa accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc
     3241 aaggactccc aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt
     3301 tgttaatcgg tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc
     3361 ttttatgatg taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag
     3421 atgtctcact tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct
     3481 gttcttatta cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat
     3541 cagctgaccc tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt
     3601 ttttctctaa tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa
     3661 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
     3721 aatttttctt actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc
     3781 agaagtaact tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc
     3841 caaattcctt cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc
     3901 actctttttt ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag
     3961 aattcgtcaa ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg
     4021 atttgcagta aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa
     4081 ttgacaaaga attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag
     4141 cttagaccgc ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg
     4201 tcagcaaaag gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt
     4261 gtaagatctt cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg
     4321 ttattatcaa atgcatggga aatctcatga gcgataacag ctccgattcc accataatta
     4381 gcagagcttg attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg
     4441 atcacattcc gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct
     4501 gaacgatcca ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa
     4561 acaagagtat ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg
     4621 aataatgctg ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg
     4681 acggcttttt cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa
     4741 acggcgatca tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat
     4801 ttttttccgt agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct
     4861 gcttttttct gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg
     4921 acttggcgga attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc
     4981 cagcttttga ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac
     5041 actggatcag ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt
     5101 ttcaagtcca gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt
     5161 ttgctgtaat ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat
     5221 tgcactgctt gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat
     5281 aactgaacag ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat
     5341 gcataatacg tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt
     5401 tgcgcattct tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac
     5461 agccattctg gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg
     5521 attataggtt gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt
     5581 tgataatatg caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc
     5641 agatcgtctg tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg
     5701 cctgtagctg gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct
     5761 tcatagaaat cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc
     5821 gtgtgcccat tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat
     5881 tcgaacaatc aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc
     5941 gatcccaact gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta
     6001 aaaaattagc cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc
     6061 tggtttttga agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt
     6121 ctccagcaaa atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg
     6181 aagagccaat cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg
     6241 aagaaaaaag gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt
     6301 ttttgaggta taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag
     6361 cagttctcct tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc
     6421 gcaagaaaaa tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac
     6481 cactaggact tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga
     6541 agaatggcaa tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa
     6601 aacgtatacg tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat
     6661 atggcaatca ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt
     6721 acaaagtaca cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg
     6781 gaacagtttc acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt
     6841 taaactatcg aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat
     6901 gacacgttga agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa
     6961 gtgttcggaa cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc
     7021 atcaatatcg aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca
     7081 cctgtcaatg attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt
     7141 gcgaaaacga tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg
     7201 aaacctcatg aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca
     7261 actcgagtac tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct
     7321 gtagataatt tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg
     7381 ggagatgaca tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt
     7441 tcagaatatt tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag
     7501 ggtggttatg tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg
     7561 aacacaggag caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa
     7621 gtatatgcct gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat
     7681 gcaccaatca aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca
     7741 gaaaacttaa caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat
     7801 gaaaatacac cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa
     7861 aaaagaggga aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt
     7921 tgtcacagct ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct
     7981 ttggtttgtt cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg
     8041 aagtgaaaac tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg
     8101 aagcgatgac ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg
     8161 gtcgccacgc gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag
     8221 caaaacctca cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct
     8281 tagaaacagc gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac
     8341 atcctgctgt tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc
     8401 tgcctgtaga tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag
     8461 atacgatctt ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca
     8521 aagaaatcgg tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag
     8581 cgtacggaaa ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg
     8641 ctcataaaat caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata
     8701 tccctgcatt gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt
     8761 tagcagggat ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag
     8821 cacgtaacaa caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg
     8881 aaatccctta tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc
     8941 gcttaattgg cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta
     9001 tttctactgg ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa
     9061 tgtatggaga agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa
     9121 ataccgaaga cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac
     9181 tagtgaagtc attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac
     9241 ttaactgaaa aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt
     9301 atcgaggtga aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc
     9361 tatcgtttgc atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca
     9421 aacggaggca attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga
     9481 ttcaaattaa aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca
     9541 gcaaatggat tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat
     9601 aaattttatt ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga
     9661 tctgtggata taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg
     9721 taaggacgac gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt
     9781 taatattttg ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt
     9841 tagggaaaaa ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta
     9901 tacaaaatga aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg
     9961 aattggaaga gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt
    10021 atggtcgtcc aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt
    10081 ttgagttttg ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga
    10141 ccgctagtgg atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg
    10201 attttcgcgc ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta
    10261 gtttttgtgt ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag
    10321 tcgattatgc cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga
    10381 atggtccaat cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc
    10441 gttcctgtca attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta
    10501 ttgatttttg tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt
    10561 tttaaagatg caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata
    10621 acgatcaatg gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg
    10681 atgacgctag attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac
    10741 gcagtcggaa tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga
    10801 atgaaaccta ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg
    10861 gttccaaacg atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt
    10921 gcaacgccat atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat
    10981 gttttaggtc gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga
    11041 ttattatttg caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc
    11101 ggaatcggga ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat
    11161 gataaagaat tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat
    11221 ggaatggagt tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg
    11281 gcaataagcc tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat
    11341 atagggcagt tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt
    11401 tgttgctcag ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt
    11461 gcgtctacgc ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca
    11521 tgaagattgc ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat
    11581 tgttgatggt cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg
    11641 tcagtaagcc taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc
    11701 cctttgatca agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt
    11761 gctttttcat attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct
    11821 tttattactg cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag
    11881 agccgcggag caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg
    11941 ataaagtaat tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat
    12001 ctgagcaact cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg
    12061 ataagaagca attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga
    12121 aaggctggcc ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg
    12181 attttttggt tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac
    12241 gacttgataa ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa
    12301 agttgcgacc ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg
    12361 acagacaaca gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta
    12421 acagcacttt tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg
    12481 gatgacacgg acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa
    12541 gtagctgctc aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat
    12601 cgtgtatttg aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg
    12661 atgtgcaaca aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc
    12721 gattatgtag ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac
    12781 atgcttcgtg gagtagacaa taataaagat caaacttact tccttagcca attatcacaa
    12841 gaacagcttt caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca
    12901 attgctgaac gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt
    12961 attggtgaaa agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat
    13021 atggtcactc ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt
    13081 ggtcaacgtc aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt
    13141 gtaggaaaag atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca
    13201 ttgtatgcca caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca
    13261 aaagaattca agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt
    13321 cgcttattgg atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact
    13381 cctggacaag cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac
    13441 cacgcatatc aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa
    13501 cagctatggc taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa
    13561 taaaattaat ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc
    13621 aataatatga ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc
    13681 ttttgcaagt gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga
    13741 cgggtattag caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg
    13801 tgtttgggat cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat
    13861 ggataatcgt tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa
    13921 agcgtccaga tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag
    13981 gtggcttcat cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg
    14041 caacgatgct gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg
    14101 ttccttctat tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg
    14161 gagttgaagg agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat
    14221 tcctattttt aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt
    14281 ggggtaacgt atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc
    14341 agattaaagg ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg
    14401 ttttagccaa ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc
    14461 tttgtttatt gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag
    14521 aagagttagc aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa
    14581 attggggagc agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt
    14641 cttggacgat gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga
    14701 aaggttgggg aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa
    14761 tcttgactaa tctattctta ttgactttct tagttactga ttatgcctat caatttgcct
    14821 attctttatg tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc
    14881 agttttcttt aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt
    14941 ttgaattgat gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct
    15001 atcttcctgg tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg
    15061 gaaaagaaaa agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc
    15121 ttgctgtagg ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt
    15181 gtagaagaat ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg
    15241 atcgaagcat tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt
    15301 ttcgaaaaac gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc
    15361 aaacaagaag tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat
    15421 ggagcgactg gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt
    15481 gtttcgatgc ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct
    15541 gttgactttg ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa
    15601 gccttagtca aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc
    15661 acattgctag atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca
    15721 tatgtattga tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc
    15781 gtagatgtct atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca
    15841 ggtattcgta ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat
    15901 cgagactaca cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg
    15961 aagaataaag aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt
    16021 ttaaaacaat gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct
    16081 acttctttca ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta
    16141 gaagaaacag gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga
    16201 ctaggttact gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc
    16261 ttgaaaaaac aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca
    16321 gtgtttttca tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact
    16381 cctgaagctt tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca
    16441 agcaagaagt tgaccaaaac aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat
    16501 gcactggaaa cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat
    16561 tggttataac tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct
    16621 tggacagcag ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat
    16681 tgccaaacat tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca
    16741 atcgcaagta ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct
    16801 atcaaagtgg caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca
    16861 tgtccatgca gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat
    16921 gcttggatga tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct
    16981 ttgaaaatct gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg
    17041 acgaaaaaca gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta
    17101 gggcgatagc caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct
    17161 gtaacagaac cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca
    17221 gttgcgacaa gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc
    17281 aatgcgacta attgatacac actagtcacg caacccatca aagaaatgac gattgccatt
    17341 cctaatgaag cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac
    17401 caaggagaga taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag
    17461 ttcaattgtg acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg
    17521 actaaactaa atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt
    17581 tcccaagttt ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct
    17641 tctttctgaa aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa
    17701 tgaaagtctc ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt
    17761 tatgagagga aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg
    17821 ctttcgagaa ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa
    17881 acgcttggat atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa
    17941 aacagcgcta acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt
    18001 gggaaaaagt ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt
    18061 ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt
    18121 attagatgga atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt
    18181 aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct
    18241 ttccttacgt tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta
    18301 tttttggatg atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa
    18361 caattttaag atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg
    18421 aaaagtgact aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta
    18481 attaccatgt tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt
    18541 gaagaagaag aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg
    18601 aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt
    18661 tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag
    18721 ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat
    18781 gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg
    18841 tgctaatcaa aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct
    18901 tggtcattta ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca
    18961 aatggacacg ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg
    19021 acatcaatta aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat
    19081 tgcgacagat gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca
    19141 tttcaataaa ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac
    19201 tggattcaag caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc
    19261 ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta
    19321 cgcggataat ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca
    19381 agaaaagaaa atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg
    19441 aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc
    19501 acaaaaactt gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa
    19561 attaaaaacg gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa
    19621 tcaacgctat ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac
    19681 ccataagatc gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag
    19741 tacgatgaaa gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt
    19801 gaaaaacgga gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca
    19861 ccatgcactt ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg
    19921 aaaaatctat caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt
    19981 cagccaaaca aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg
    20041 tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga
    20101 aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa
    20161 accattgtca gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc
    20221 tacgcgtgca gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca
    20281 aggactgcaa gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt
    20341 aacaccagat ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa
    20401 acaagcagga accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat
    20461 caaatcaagc aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg
    20521 tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc
    20581 agattgcaac taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg
    20641 tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga
    20701 tggaacgaca gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga
    20761 agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc
    20821 agcagcacta aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt
    20881 ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
    20941 ttcggcttat ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt
    21001 gacaagactt ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat
    21061 attattggtg aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa
    21121 caggacgctg tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac
    21181 ataacaattg gattccacca atgaacagga agatcgagac catggaaggc catcctgatg
    21241 taggatcacc aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga
    21301 cacaagacaa agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga
    21361 taccatcaat cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac
    21421 gttcgcggtt ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt
    21481 ttgaaaatcg gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca
    21541 agcggaaatc acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt
    21601 agaatttttt cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg
    21661 tgccgacaca atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg
    21721 gcggatcttg cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc
    21781 cagcgtacag agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag
    21841 gataagctga ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa
    21901 taaacaaata ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag
    21961 gaacagtttt ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc
    22021 tttcaatcgt atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc
    22081 tttaaaattt gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt
    22141 ttaccatgtt tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag
    22201 ttttggatag agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa
    22261 atacacacac ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta
    22321 tcttttcagc caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa
    22381 gaacagtttg aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata
    22441 aagagaggta cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga
    22501 gaaatccgaa cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg
    22561 attgggcagg caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc
    22621 gggcaaaatg gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa
    22681 cgccaattgt tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat
    22741 tgttccatgc gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa
    22801 taaatagtaa agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc
    22861 gtgtaccttc tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag
    22921 tgatctcaaa ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa
    22981 acaagctgtt gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt
    23041 ttcgtttttt ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga
    23101 atttttcaaa cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca
    23161 tggcgaattc gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag
    23221 attggagtgt accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta
    23281 atccaagaaa agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga
    23341 tctgtccttt ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag
    23401 aaccacaaac agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt
    23461 ttttaccaat caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa
    23521 ttccttttac accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa
    23581 cagagaattc taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt
    23641 taaaaaaagt atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact
    23701 gtggcagata aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac
    23761 cgggtaagat ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt
    23821 tcatttgatc ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact
    23881 aaaccagtat tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt
    23941 ttcgaataaa cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa
    24001 tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg
    24061 acctcttggt aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa
    24121 aaacatgaac agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc
    24181 acaacctatt gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta
    24241 tgttctttaa ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca
    24301 aacagacatt ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg
    24361 aagtaatgaa cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct
    24421 gcatagcttg gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa
    24481 tacaatactt gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag
    24541 tttttaagat aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga
    24601 cgtgtcggat gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag
    24661 aacgcatagc agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc
    24721 ccggcagtta cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt
    24781 gcttcaattg aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc
    24841 acttggaaga taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat
    24901 gaaacaaaat aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg
    24961 atgattggat catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa
    25021 atgaatatca taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga
    25081 tagtctttaa acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc
    25141 ggaataattt tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta
    25201 gtaccaagat gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag
    25261 acagctgatt gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt
    25321 ctattataga tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa
    25381 aagttatgga agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac
    25441 aagaaatttt tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca
    25501 aatcaaacag ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga
    25561 gatgcttcca actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga
    25621 atgggaagaa actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat
    25681 cgttgcttcc cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa
    25741 aaggtttcct aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca
    25801 agtagcgaaa catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt
    25861 acaaagatac tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt
    25921 gtggcttgta agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga
    25981 gatgaatatc caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct
    26041 gaaggaagga atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat
    26101 gactccgttg aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga
    26161 tcagctgcta gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca
    26221 cagcgaggag aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca
    26281 ctaaagataa gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag
    26341 ttaatttttc ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata
    26401 atcagcagta tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga
    26461 acgataatcg tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc
    26521 tgctctcatt atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg
    26581 tagggatgca agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt
    26641 tgaattccaa atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa
    26701 tgggactctt gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact
    26761 ggtttcgatt cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct
    26821 tgatgatggc actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat
    26881 cggacttttg gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca
    26941 tggcccaaga tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc
    27001 tgatgtctgg aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg
    27061 gagcaggaac gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg
    27121 gattcaaatc ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt
    27181 caggaatgag ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg
    27241 gtaaaggatt caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta
    27301 ccgttatcgg tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta
    27361 ttttaatcta tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata
    27421 tcgcaagcgg tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata
    27481 tcggattact tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga
    27541 ttttagggaa catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac
    27601 ctgcaagatc ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc
    27661 aacatgcaaa aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga
    27721 tatgatcaaa gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt
    27781 accaatccgt cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga
    27841 tcaagtaggc gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct
    27901 gatcaagcga ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa
    27961 aatttatgaa ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc
    28021 tttggatctt atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag
    28081 aacttggaga catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg
    28141 aacctctgat cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg
    28201 gtacagtttc ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag
    28261 atgaaatgaa tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat
    28321 tataaaatca gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca
    28381 taagcaatcc tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt
    28441 gaagagagcc ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag
    28501 cagagtaagc gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata
    28561 cggaaagaag ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca
    28621 aggtggtaac acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct
    28681 tttttgtttg aaaaattata agagaaagat gtgaatggca tgtcattaat ccaactaacg
    28741 aatgtcaaaa agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca
    28801 ctaaacgttg aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt
    28861 acattagtcc gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac
    28921 gatcaagata ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt
    28981 atgatcttcc agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg
    29041 ccgctggaat tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg
    29101 cgtttagtcg ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag
    29161 aaacaacgcg tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat
    29221 gaggctacta gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg
    29281 atcaataaga aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc
    29341 aaaatctgca acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta
    29401 ttgactgtct tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc
    29461 gaaccagaag ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc
    29521 cgtttggcaa ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg
    29581 attcgacgct ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa
    29641 ggttcctttg gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca
    29701 gaagtgttca tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt
    29761 ttcagaaaca tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct
    29821 tgatacacta tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt
    29881 gggattgatc ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg
    29941 aatcgtatcg atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt
    30001 gatcattcca tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc
    30061 agctcttgtt ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga
    30121 agtcagccct ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat
    30181 ctggaaggtc ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac
    30241 gatctcgatg atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg
    30301 tcttgcttgg caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt
    30361 aattatttta ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac
    30421 agacaaaaga taataggata tagggggatg taggatatga aaaagaaaat ttttggattt
    30481 gcagcagcgc ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa
    30541 gattcagcaa ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac
    30601 gcagagattt tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc
    30661 gtcaaattcg atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc
    30721 aattacttcc agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt
    30781 gtcaatgccg gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat
    30841 gtttccgaat taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga
    30901 cgcatcatca caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg
    30961 gaaacagcaa cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg
    31021 atcgacccat cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc
    31081 aacgcaaact tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa
    31141 aaggacaact ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac
    31201 aaaatcaaaa aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac
    31261 aaatggggcg gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa
    31321 agatgagaaa taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta
    31381 gaaatcaaaa ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg
    31441 cgcttcattc tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg
    31501 catgtctgtc ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa
    31561 aggcgtaaac ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac
    31621 aggaaaatct accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg
    31681 cgaaattttg tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact
    31741 tggcttattt ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt
    31801 catgcgtgca gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt
    31861 tttgaaaaag ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg
    31921 ttatctgaac gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt
    31981 gatgttagaa cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc
    32041 tttgaaagtc gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat
    32101 catcacccac tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat
    32161 ggaaggccga gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg
    32221 ctacgctggt atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg
    32281 agcaagaatg aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt
    32341 aagtaaaggc gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga
    32401 tgaactgcca ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt
    32461 gaaagaaaca caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga
    32521 caatcctgtg cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt
    32581 agcagaaaaa ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact
    32641 agtccaagaa tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact
    32701 ccatgtagct tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga
    32761 tgaaccactc gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca
    32821 tgtgttgatc gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac
    32881 caaagaacaa gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg
    32941 ttcaaaaatc aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa
    33001 tcgccgtggg cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga
    33061 cggtcatgtg atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt
    33121 caaaatcgtg gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa
    33181 taaagcccct catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac
    33241 attgaccttt aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca
    33301 agaaagtcgt gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt
    33361 gatcgatgaa aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga
    33421 agaaatgtac tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat
    33481 tcgaggattc ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt
    33541 agtcgaagta atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat
    33601 tttcctatct tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg
    33661 acgacccaaa agccaaaaca agtactccag gcattagcgt actattacga acacgataac
    33721 gccaatgttc atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa
    33781 gcaagagaaa aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact
    33841 cgtggtacga cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa
    33901 gcaggagacg agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag
    33961 cagttggcaa aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc
    34021 ctggatatgg aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct
    34081 catgtatcca atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat
    34141 gaaaacggcg gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat
    34201 gtccaagcac tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca
    34261 ggtattggtg ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt
    34321 ggtggcgaaa tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg
    34381 aaattcgaag cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat
    34441 tatttgcaaa aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat
    34501 gtgttgccaa aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat
    34561 tttcggacag gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca
    34621 gctttagaca tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg
    34681 aagtaccttg aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa
    34741 gacgccgacc gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact
    34801 atctaaatta gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca
    34861 tcgtggaagc ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga
    34921 tgtgatcgaa ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg
    34981 cagtggctgc tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa
    35041 atcaatagaa gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga
    35101 agtcaaagat cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc
    35161 cgctcggatc aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga
    35221 tggtaccgca gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag
    35281 aattagaaga atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag
    35341 aaggcttgac agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga
    35401 tgcttgaatt ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg
    35461 gccccgacct ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca
    35521 aacccgctcg tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg
    35581 gtataccaga agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag
    35641 tcgtgtacca taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg
    35701 actctgcttt gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc
    35761 caaccgacaa caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt
    35821 acgtgccaaa aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg
    35881 aaaacacggg gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact
    35941 atgtcgaagg ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag
    36001 aaatctttac aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca
    36061 atgtgtacaa cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga
    36121 tcgacggaaa cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa
    36181 aaggtgcacg aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata
    36241 cgggtgcaaa aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa
    36301 tttcaaaaga cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg
    36361 cgggttcgat ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg
    36421 atacgatccc attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag
    36481 tctcaaaaat ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg
    36541 aagcaactga aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg
    36601 aatatgcagt cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa
    36661 cctgttatag gagctagaac ccttgatata
//
LOCUS       DANKBF010000023        34962 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000023 DANKBF010000000
VERSION     DANKBF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="PWY89_001239"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="PWY89_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL2066287.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="PWY89_001240"
     CDS             complement(2020..2922)
                     /locus_tag="PWY89_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2066288.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="PWY89_001241"
     CDS             complement(2919..3611)
                     /locus_tag="PWY89_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL2066289.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="PWY89_001242"
     CDS             complement(3654..4295)
                     /locus_tag="PWY89_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2066290.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="PWY89_001243"
     CDS             complement(4333..4647)
                     /locus_tag="PWY89_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066291.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="PWY89_001244"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="PWY89_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="PWY89_001245"
     CDS             complement(5984..6916)
                     /locus_tag="PWY89_001245"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2066292.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="PWY89_001246"
     CDS             complement(6913..7689)
                     /locus_tag="PWY89_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL2066293.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PWY89_001247"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PWY89_001247"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2066294.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PWY89_001248"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PWY89_001248"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2066295.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="PWY89_001249"
     CDS             complement(12014..13294)
                     /locus_tag="PWY89_001249"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL2066296.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="PWY89_001250"
     CDS             complement(13313..14239)
                     /locus_tag="PWY89_001250"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL2066297.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="PWY89_001251"
     CDS             complement(14252..15535)
                     /locus_tag="PWY89_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL2066298.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PWY89_001252"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PWY89_001252"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2066299.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="PWY89_001253"
     CDS             complement(16323..17234)
                     /locus_tag="PWY89_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2066300.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="PWY89_001254"
     CDS             complement(17231..17695)
                     /locus_tag="PWY89_001254"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2066301.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="PWY89_001255"
     CDS             complement(17700..18398)
                     /locus_tag="PWY89_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2066302.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="PWY89_001256"
     CDS             complement(18385..18771)
                     /locus_tag="PWY89_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066303.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="PWY89_001257"
     CDS             complement(18918..20588)
                     /locus_tag="PWY89_001257"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL2066304.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="PWY89_001258"
     CDS             20771..21004
                     /locus_tag="PWY89_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL2066305.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="PWY89_001259"
     CDS             complement(21103..21534)
                     /locus_tag="PWY89_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL2066306.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="PWY89_001260"
     CDS             21613..22026
                     /locus_tag="PWY89_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL2066307.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="PWY89_001261"
     CDS             complement(22064..23212)
                     /locus_tag="PWY89_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2066308.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="PWY89_001262"
     CDS             complement(23334..23930)
                     /locus_tag="PWY89_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2066309.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLSSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PWY89_001263"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PWY89_001263"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2066310.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="PWY89_001264"
     CDS             27160..27591
                     /locus_tag="PWY89_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2066311.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="PWY89_001265"
     CDS             complement(27636..28985)
                     /locus_tag="PWY89_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2066312.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="PWY89_001266"
     CDS             complement(29055..30218)
                     /locus_tag="PWY89_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2066313.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="PWY89_001267"
     CDS             30347..30670
                     /locus_tag="PWY89_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066314.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="PWY89_001268"
     CDS             complement(30853..32202)
                     /locus_tag="PWY89_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2066315.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="PWY89_001269"
     CDS             complement(32271..33224)
                     /locus_tag="PWY89_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL2066316.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="PWY89_001270"
     CDS             complement(33326..34516)
                     /locus_tag="PWY89_001270"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL2066317.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9952 a         7641 c         6120 g        11249 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaactc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DANKBF010000024        34789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000024 DANKBF010000000
VERSION     DANKBF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(233..1081)
                     /locus_tag="PWY89_001271"
     CDS             complement(233..1081)
                     /locus_tag="PWY89_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2066318.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="PWY89_001272"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="PWY89_001272"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2066319.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="PWY89_001273"
     CDS             complement(3582..3761)
                     /locus_tag="PWY89_001273"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL2066320.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="PWY89_001274"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="PWY89_001274"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2066321.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="PWY89_001275"
     CDS             5909..8188
                     /locus_tag="PWY89_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2066322.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="PWY89_001276"
     CDS             complement(8281..8928)
                     /locus_tag="PWY89_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066323.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="PWY89_001277"
     CDS             complement(9107..9826)
                     /locus_tag="PWY89_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2066324.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="PWY89_001278"
     CDS             complement(9957..11108)
                     /locus_tag="PWY89_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL2066325.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="PWY89_001279"
     CDS             complement(11325..12065)
                     /locus_tag="PWY89_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2066326.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="PWY89_001280"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="PWY89_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2066327.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="PWY89_001281"
     CDS             complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="PWY89_001281"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL2066328.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13676)
                     /locus_tag="PWY89_001282"
     CDS             complement(13026..13676)
                     /locus_tag="PWY89_001282"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL2066329.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="PWY89_001283"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="PWY89_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2066330.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="PWY89_001284"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="PWY89_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL2066331.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="PWY89_001285"
     CDS             complement(15119..15646)
                     /locus_tag="PWY89_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL2066332.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="PWY89_001286"
     CDS             complement(15826..16611)
                     /locus_tag="PWY89_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2066333.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="PWY89_001287"
     CDS             complement(16604..17473)
                     /locus_tag="PWY89_001287"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL2066334.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="PWY89_001288"
     CDS             complement(17474..18853)
                     /locus_tag="PWY89_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2066335.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="PWY89_001289"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="PWY89_001289"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2066336.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="PWY89_001290"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="PWY89_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2066337.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="PWY89_001291"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="PWY89_001291"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL2066338.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="PWY89_001292"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="PWY89_001292"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL2066339.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="PWY89_001293"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="PWY89_001293"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2066340.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="PWY89_001294"
     CDS             complement(22811..23035)
                     /locus_tag="PWY89_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2066341.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="PWY89_001295"
     CDS             complement(23088..24050)
                     /locus_tag="PWY89_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2066342.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="PWY89_001296"
     CDS             complement(24060..24500)
                     /locus_tag="PWY89_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2066343.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="PWY89_001297"
     CDS             24806..25771
                     /locus_tag="PWY89_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2066344.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="PWY89_001298"
     CDS             complement(25828..26058)
                     /locus_tag="PWY89_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL2066345.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="PWY89_001299"
     CDS             complement(26144..27031)
                     /locus_tag="PWY89_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2066346.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="PWY89_001300"
     CDS             complement(27120..28343)
                     /locus_tag="PWY89_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2066347.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29406)
                     /locus_tag="PWY89_001301"
     CDS             complement(28360..29406)
                     /locus_tag="PWY89_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2066348.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="PWY89_001302"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="PWY89_001302"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL2066349.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="PWY89_001303"
     CDS             complement(31297..31563)
                     /locus_tag="PWY89_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL2066350.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="PWY89_001304"
     CDS             32042..34276
                     /locus_tag="PWY89_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2066351.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="PWY89_001305"
     CDS             34424..34744
                     /locus_tag="PWY89_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2066352.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9843 a         7510 c         6063 g        11373 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcgtatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atagtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctc aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaa
//
LOCUS       DANKBF010000025        34580 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000025 DANKBF010000000
VERSION     DANKBF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            212..604
                     /locus_tag="PWY89_001306"
     CDS             212..604
                     /locus_tag="PWY89_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2066353.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="PWY89_001307"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="PWY89_001307"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2066354.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="PWY89_001308"
     CDS             3165..4775
                     /locus_tag="PWY89_001308"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL2066355.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="PWY89_001309"
     CDS             5067..5936
                     /locus_tag="PWY89_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL2066356.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="PWY89_001310"
     CDS             6101..7372
                     /locus_tag="PWY89_001310"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2066357.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="PWY89_001311"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="PWY89_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2066358.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="PWY89_001312"
     CDS             8763..9026
                     /locus_tag="PWY89_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2066359.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="PWY89_001313"
     CDS             9471..9920
                     /locus_tag="PWY89_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2066360.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="PWY89_001314"
     CDS             9977..11395
                     /locus_tag="PWY89_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2066361.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="PWY89_001315"
     CDS             11395..12255
                     /locus_tag="PWY89_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL2066362.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="PWY89_001316"
     CDS             12429..13202
                     /locus_tag="PWY89_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2066363.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="PWY89_001317"
     CDS             13349..13714
                     /locus_tag="PWY89_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL2066364.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="PWY89_001318"
     CDS             complement(13862..15070)
                     /locus_tag="PWY89_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2066365.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="PWY89_001319"
     CDS             complement(15089..15367)
                     /locus_tag="PWY89_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066366.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="PWY89_001320"
     CDS             15521..16216
                     /locus_tag="PWY89_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2066367.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="PWY89_001321"
     CDS             complement(16658..17455)
                     /locus_tag="PWY89_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2066368.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="PWY89_001322"
     CDS             complement(17452..18636)
                     /locus_tag="PWY89_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2066369.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="PWY89_001323"
     CDS             complement(18661..19908)
                     /locus_tag="PWY89_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2066370.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="PWY89_001324"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="PWY89_001324"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2066371.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="PWY89_001325"
     CDS             21888..22202
                     /locus_tag="PWY89_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2066372.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="PWY89_001326"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="PWY89_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2066373.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="PWY89_001327"
     CDS             22887..23531
                     /locus_tag="PWY89_001327"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2066374.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="PWY89_001328"
     CDS             23546..23875
                     /locus_tag="PWY89_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL2066375.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="PWY89_001329"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="PWY89_001329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2066376.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="PWY89_001330"
     CDS             24863..25687
                     /locus_tag="PWY89_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL2066377.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="PWY89_001331"
     CDS             25680..26027
                     /locus_tag="PWY89_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL2066378.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="PWY89_001332"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="PWY89_001332"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2066379.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="PWY89_001333"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="PWY89_001333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2066380.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWY89_001334"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWY89_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2066381.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWY89_001335"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWY89_001335"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2066382.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="PWY89_001336"
     CDS             31657..32451
                     /locus_tag="PWY89_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066383.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="PWY89_001337"
     CDS             32441..34483
                     /locus_tag="PWY89_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2066384.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11118 a         6130 c         7259 g        10073 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta
//
LOCUS       DANKBF010000026        32497 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000026 DANKBF010000000
VERSION     DANKBF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..563)
                     /locus_tag="PWY89_001338"
     CDS             complement(<1..563)
                     /locus_tag="PWY89_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL2066385.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="PWY89_001339"
     CDS             complement(750..1400)
                     /locus_tag="PWY89_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066386.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="PWY89_001340"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="PWY89_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2066387.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="PWY89_001341"
     CDS             complement(2912..3910)
                     /locus_tag="PWY89_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2066388.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="PWY89_001342"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="PWY89_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2066389.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="PWY89_001343"
     CDS             complement(4926..5657)
                     /locus_tag="PWY89_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2066390.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="PWY89_001344"
     CDS             complement(5661..6926)
                     /locus_tag="PWY89_001344"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2066391.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="PWY89_001345"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="PWY89_001345"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2066392.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="PWY89_001346"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="PWY89_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2066393.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="PWY89_001347"
     CDS             12268..13770
                     /locus_tag="PWY89_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2066394.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="PWY89_001348"
     CDS             13772..14509
                     /locus_tag="PWY89_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2066395.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="PWY89_001349"
     CDS             complement(14680..15873)
                     /locus_tag="PWY89_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2066396.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="PWY89_001350"
     CDS             complement(15952..16158)
                     /locus_tag="PWY89_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2066397.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="PWY89_001351"
     CDS             complement(16151..17293)
                     /locus_tag="PWY89_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2066398.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="PWY89_001352"
     CDS             17805..18440
                     /locus_tag="PWY89_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2066399.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="PWY89_001353"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="PWY89_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18916..19032
                     /locus_tag="PWY89_001354"
     CDS             18916..19032
                     /locus_tag="PWY89_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL2066400.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="PWY89_001355"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="PWY89_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDL2066401.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="PWY89_001356"
     CDS             21086..21253
                     /locus_tag="PWY89_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL2066402.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="PWY89_001357"
     CDS             complement(21313..21666)
                     /locus_tag="PWY89_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066403.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="PWY89_001358"
     CDS             22170..22589
                     /locus_tag="PWY89_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2066404.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="PWY89_001359"
     CDS             22615..22821
                     /locus_tag="PWY89_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066405.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="PWY89_001360"
     CDS             complement(23049..23222)
                     /locus_tag="PWY89_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066406.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23198..23863
                     /locus_tag="PWY89_001361"
     CDS             23198..23863
                     /locus_tag="PWY89_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066407.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="PWY89_001362"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="PWY89_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2066408.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="PWY89_001363"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="PWY89_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL2066409.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="PWY89_001364"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="PWY89_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2066410.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="PWY89_001365"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="PWY89_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL2066411.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /locus_tag="PWY89_001366"
                     /pseudo
     CDS             complement(29076..30241)
                     /locus_tag="PWY89_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(30257..30484)
                     /locus_tag="PWY89_001367"
     CDS             complement(30257..30484)
                     /locus_tag="PWY89_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL2066412.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31558
                     /locus_tag="PWY89_001368"
     CDS             30968..31558
                     /locus_tag="PWY89_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2066413.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            31748..32461
                     /locus_tag="PWY89_001369"
     CDS             31748..32461
                     /locus_tag="PWY89_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066414.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9520 a         6618 c         5736 g        10623 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagc
//
LOCUS       DANKBF010000027        30945 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000027 DANKBF010000000
VERSION     DANKBF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..420
                     /locus_tag="PWY89_001370"
     CDS             <1..420
                     /locus_tag="PWY89_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2066415.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="PWY89_001371"
     CDS             complement(504..1226)
                     /locus_tag="PWY89_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2066416.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="PWY89_001372"
     CDS             complement(1216..1953)
                     /locus_tag="PWY89_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066417.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="PWY89_001373"
     CDS             complement(1965..2957)
                     /locus_tag="PWY89_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2066418.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="PWY89_001374"
     CDS             complement(2972..3715)
                     /locus_tag="PWY89_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2066419.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="PWY89_001375"
     CDS             complement(3956..5278)
                     /locus_tag="PWY89_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2066420.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="PWY89_001376"
     CDS             complement(5265..6230)
                     /locus_tag="PWY89_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066421.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="PWY89_001377"
     CDS             complement(6227..7765)
                     /locus_tag="PWY89_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL2066422.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="PWY89_001378"
     CDS             complement(8181..8468)
                     /locus_tag="PWY89_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066423.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="PWY89_001379"
     CDS             8602..9270
                     /locus_tag="PWY89_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2066424.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="PWY89_001380"
     CDS             complement(9561..10301)
                     /locus_tag="PWY89_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066425.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="PWY89_001381"
     CDS             complement(10431..11378)
                     /locus_tag="PWY89_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2066426.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="PWY89_001382"
     CDS             complement(11507..12271)
                     /locus_tag="PWY89_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2066427.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="PWY89_001383"
     CDS             complement(12275..12664)
                     /locus_tag="PWY89_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066428.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="PWY89_001384"
     CDS             12799..13341
                     /locus_tag="PWY89_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL2066429.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="PWY89_001385"
     CDS             13489..14121
                     /locus_tag="PWY89_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066430.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="PWY89_001386"
     CDS             complement(14255..14380)
                     /locus_tag="PWY89_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2066431.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="PWY89_001387"
     CDS             complement(14514..15407)
                     /locus_tag="PWY89_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL2066432.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="PWY89_001388"
     CDS             complement(15589..16515)
                     /locus_tag="PWY89_001388"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL2066433.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PWY89_001389"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PWY89_001389"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL2066434.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PWY89_001390"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PWY89_001390"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2066435.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PWY89_001391"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PWY89_001391"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2066436.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PWY89_001392"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PWY89_001392"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2066437.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="PWY89_001393"
     CDS             complement(24786..25220)
                     /locus_tag="PWY89_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL2066438.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="PWY89_001394"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="PWY89_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2066439.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="PWY89_001395"
     CDS             complement(26117..26992)
                     /locus_tag="PWY89_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2066440.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PWY89_001396"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PWY89_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL2066441.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PWY89_001397"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PWY89_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL2066442.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PWY89_001398"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PWY89_001398"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL2066443.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PWY89_001399"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PWY89_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2066444.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         8535 a         6510 c         5272 g        10628 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcta tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgtaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaa
//
LOCUS       DANKBF010000028        30787 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000028 DANKBF010000000
VERSION     DANKBF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(133..297)
                     /locus_tag="PWY89_001400"
     CDS             complement(133..297)
                     /locus_tag="PWY89_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066445.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="PWY89_001401"
     CDS             592..1920
                     /locus_tag="PWY89_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL2066446.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="PWY89_001402"
     CDS             2122..2955
                     /locus_tag="PWY89_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL2066447.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="PWY89_001403"
     CDS             2971..3708
                     /locus_tag="PWY89_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL2066448.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="PWY89_001404"
     CDS             complement(3863..4831)
                     /locus_tag="PWY89_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2066449.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="PWY89_001405"
     CDS             5063..5896
                     /locus_tag="PWY89_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2066450.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="PWY89_001406"
     CDS             5930..6769
                     /locus_tag="PWY89_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2066451.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="PWY89_001407"
     CDS             6795..7355
                     /locus_tag="PWY89_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2066452.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="PWY89_001408"
     CDS             7554..9275
                     /locus_tag="PWY89_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL2066453.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="PWY89_001409"
     CDS             9440..10582
                     /locus_tag="PWY89_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2066454.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="PWY89_001410"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="PWY89_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2066455.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12382..13029
                     /locus_tag="PWY89_001411"
                     /pseudo
     CDS             12382..13029
                     /locus_tag="PWY89_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
     misc_binding    13158..13374
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13422..16067
                     /locus_tag="PWY89_001412"
     CDS             13422..16067
                     /locus_tag="PWY89_001412"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2066456.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16377..17690
                     /locus_tag="PWY89_001413"
     CDS             16377..17690
                     /locus_tag="PWY89_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2066457.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17680..18333
                     /locus_tag="PWY89_001414"
     CDS             17680..18333
                     /locus_tag="PWY89_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2066458.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18388..19080
                     /gene="radC"
                     /locus_tag="PWY89_001415"
     CDS             18388..19080
                     /gene="radC"
                     /locus_tag="PWY89_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL2066459.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19230..19430
                     /locus_tag="PWY89_001416"
     CDS             19230..19430
                     /locus_tag="PWY89_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2066460.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19684..21363)
                     /locus_tag="PWY89_001417"
     CDS             complement(19684..21363)
                     /locus_tag="PWY89_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2066461.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21365..21577)
                     /locus_tag="PWY89_001418"
     CDS             complement(21365..21577)
                     /locus_tag="PWY89_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2066462.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21970..23700
                     /gene="efrA"
                     /locus_tag="PWY89_001419"
     CDS             21970..23700
                     /gene="efrA"
                     /locus_tag="PWY89_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL2066463.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23697..25457
                     /gene="efrB"
                     /locus_tag="PWY89_001420"
     CDS             23697..25457
                     /gene="efrB"
                     /locus_tag="PWY89_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL2066464.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25552..25749
                     /locus_tag="PWY89_001421"
     CDS             25552..25749
                     /locus_tag="PWY89_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066465.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25902..26387
                     /locus_tag="PWY89_001422"
     CDS             25902..26387
                     /locus_tag="PWY89_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL2066466.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26491..27084)
                     /gene="clpP"
                     /locus_tag="PWY89_001423"
     CDS             complement(26491..27084)
                     /gene="clpP"
                     /locus_tag="PWY89_001423"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2066467.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27264..27791
                     /locus_tag="PWY89_001424"
     CDS             27264..27791
                     /locus_tag="PWY89_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066468.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27882..28619
                     /locus_tag="PWY89_001425"
     CDS             27882..28619
                     /locus_tag="PWY89_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2066469.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28623..29540
                     /gene="miaA"
                     /locus_tag="PWY89_001426"
     CDS             28623..29540
                     /gene="miaA"
                     /locus_tag="PWY89_001426"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2066470.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29542..30771
                     /gene="hflX"
                     /locus_tag="PWY89_001427"
     CDS             29542..30771
                     /gene="hflX"
                     /locus_tag="PWY89_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2066471.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10170 a         5264 c         6568 g         8785 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt gcgaaaaagc
    12181 tgccctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat tttacgtttg
    12241 gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt tttatttgtc tcacaccctt
    12301 ttccttccta cttgtaacct tgctcccgtg ttagaaacat gttacactaa atatgtgaaa
    12361 ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata cactgccaaa agcaactgcg
    12421 aaaagattac ctttatattt acgctacttg aagatgctgg atgactcagg tataagtaga
    12481 atcaaatcaa atgagttcag cgaaatcacc caaattcctt ctgcaacgat ccgcagagac
    12541 ttctcgcaat taggcgagct aggcagaagc ggatatggct atgatgtccc ttttcttatc
    12601 gatgtattca ataatatcct taatactaaa gaagaaaaac gaatcgcatt agtaggatat
    12661 ggaaatcttg gcaaagcatt gaaacacaac aattttagaa gaaatgaaaa tctaaatatc
    12721 gtgtgcgtct ttgataatga tccagcactg atcaatcgag taattgatgg agaaatgatc
    12781 tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa atgtgacagt agcaatctct
    12841 acggtaccaa gtaaatatta acaatctgct attgatgaaa ttgtcaaagg aaatgtaacc
    12901 gctatactta attttgcgcc tgacagagta actgtcccag cttatgtcaa tgttcaatac
    12961 attgatttga caaccgagtt gcagacgctt atttactttg atgagaatta ctcagaagtt
    13021 ttttcttagg aaaatttgag taggcttatt gatcttgcga gaactcgtcg taattctttc
    13081 aaaaattttt tagtactaaa tgaagtggtt ttttcacgac cacttgttga ctaaaaaaaa
    13141 aaaccattgt agaatgaagc ataacgctga acaagaggag taactggcac attcattctt
    13201 agagagagaa ctatctgctg aaaggttctc aatgacaacc agtgaaggta gcttgggagt
    13261 ttctgtcttg aatggagtag agatagacga gtcgtgatcg ttaactcact tgagaggcaa
    13321 taatgattga ttattgcaat ttaggtggta ccgcgtaaat catacgtcct agaatacgaa
    13381 agtattttag gacgtttttt agtttaaagg aggaaaacag catgacggaa gaaaataacc
    13441 tatcaacaaa atataatccg caagaagttg aagctggacg ttatcagcat tggttagaac
    13501 aagatttgtt taaaccgagc ggagataaaa aagcaaaacc ttattcaatc gtaatcccgc
    13561 ccccaaatgt tactggaaaa cttcatttag ggcatgcttg ggatacgacc ttgcaagata
    13621 tgctgattcg tcaaaagaga atgcaaggat atgatacttt atggctgcct ggtatggacc
    13681 atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct tagagagcaa gggatttctc
    13741 gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg ggaatggaaa gaagagtatg
    13801 cgagccacat ccgcgaacag tgggctaaaa tgggcttatc tttagattac agtagggaac
    13861 gttttacatt agatgatgga ttatctgaag cagtacgtaa agtttttgtt accttatacg
    13921 aaaaaggttt gatttatcgt ggtgaatata tcatcaactg ggatcctcaa gcacgaacag
    13981 ctctatctga tatcgaagtt attcataaag atatcgaagg cgccttttat catatgagct
    14041 atgaactagc agatggatca ggcgtagtag aaattgcgac aacccgtcca gaaacgatgt
    14101 tgggagatac ggcgattgct gttcatccag aagacgagcg ttatcaagcg ctcatcggca
    14161 aaaaagtgat tctaccttta gtcaataaac aaatcccaat cattgcagat gaatatgtag
    14221 atatggaatt cgggacaggt gtagtaaaaa tcactccagc acatgatcca aacgactttg
    14281 aggtaggtaa tcgtcatgat cttccacgcg taaatgttat ggatgatgat ggtacgatga
    14341 atgaactagc aggaaaatat gctggaatgg aacgttttgc tgctcgtaaa gcaatcgtgc
    14401 aagatctgaa agaaatcggt cgattgatta aaatcgatac gatgatccac agtgtgggtc
    14461 attccgagcg gacaggtgtc gttgttgaac ctcggctgtc tactcaatgg tttgtaaaaa
    14521 tggcgccgtt agcgaaaaaa gccattgata accaagaaac agatcaagca gtggaattct
    14581 tccctccaag attcaatcaa acgtttttac gttggatgga aaatgtccat gattgggtaa
    14641 tctcacgaca attatggtgg ggacatcaaa ttcctgcatg gtatcacaaa gaaacaggtg
    14701 aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa ctgggtacaa gatcctgatg
    14761 ttttagacac ttggtttagt tcagctttgt ggcctttctc aacaatgggc tggccagata
    14821 cggaaagcga agattataag cgatacttcc ctactagcac tttagtaacc ggttatgaca
    14881 tcattttctt ctgggtaagc agaatgattt tccaaagctt ggaatttact ggcgaacgtc
    14941 catttgagaa tgtcttgatt catggattga ttcgtgacga acaagggcgg aagatgagta
    15001 aatcattagg taatggaatc gatccgatgg aagtaatcga aaagtatgga gcagatgctc
    15061 tccgctggtt cttatcgaat ggttctgcac caggacaaga tgtccgcttc agctatgaaa
    15121 aaatggatgc ttcttggaac tttatcaaca aaatctggaa tgcttcacgt ttcgttatca
    15181 tgaatatcga aggaatgacc gtagaagaga tcgatttatc tggagaaaaa tcagtggctg
    15241 accggtggat ccttactcgt ttgaatgaaa cgatcgaacg agtaactgag ctatttgatc
    15301 gttttgaatt tggtgaagca ggtcgacaac tgtataactt tatctgggat gatttttgtg
    15361 actggtacat cgaaatgagt aaagaagtac tttatggaga tgatgctgtt tcaaaacaaa
    15421 ccacccgtag catcttagtc catacacttg atcagatcct acgtttattg catccaatca
    15481 tgccattcgt tacagaagaa atatgggaac atattcctca tcagggaaat tctctagtag
    15541 tggcagaata tcctgttgta cgtccagagt ttgatgatga aacagcttct aaagggatgg
    15601 aagtattgaa agaattgatt cgagccgttc gaaatattcg ttcagaagtg aatacaccat
    15661 tgtctaaacc aattacgttg ctaattaaaa caaacgatgc taaaatcgat caatttttag
    15721 ttgaaaatac aagctatatc gaacgcttct gtaatcccga agagttgacg atatcaagtg
    15781 agatcactgc gccagatcta gcgatgtcag ctgttctgac aggtgcagaa atcttcttgc
    15841 cgttagcagg attgatcaat attgaagaag aaatcaaacg attagaaaaa gaactggaaa
    15901 agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc taatgaacgt tttgtttcta
    15961 acgcgccgga agaagtagta gaggcagagc gtgcaaaaga aaaagattat ttagagaagc
    16021 aaacggctgt caaagaacga atcgaaagtt tgcgtactat tcaataagaa tagaaaaaga
    16081 gagtgtgaaa tcagctagct attactaaaa gctagctgat tgaacatcgt tacaggaaag
    16141 agtttgagcc tcactcttag cttcgattca taaggagatg tgacataaat tttgtcgcat
    16201 ctctttttta gtctactctt attatgtagg agaaggaggg aggtggatca tgtatcaaga
    16261 gtcaataaag tagttgataa aatcgttcct ctctctgttt cttttctctt tgtcatactt
    16321 ttatttttct tatgtgttct atttgttata ataaaagaga aatgggggaa ttgttgatga
    16381 cgattgaaga agcaatagaa tggatccata gtcgattacc atttggttca cgtcctggcc
    16441 ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc agagagaaaa gtaaaaacga
    16501 tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc ttatttgaga agcctattag
    16561 aagaggctgg gctgacagta ggtacattta cttcacctta tatcgaaacg ttcaatgaac
    16621 ggatcgcaat caatggcaaa agcattccag acaatgattt gatccagctt gtaaaaaaat
    16681 atcagccact gatcaaacaa cttgatcaat ttgaagcagt agctggtatt actgaattcg
    16741 agacattaac agcaatggca tttgattatt ttttgatgaa gaaagtggac gtagcgatta
    16801 tcgaagttgg cttaggcggc ttattggatt ctaccaatgt agctgtacca atgctgacgg
    16861 gaataacaac gattggactt gatcataccg aaatacttgg agatacaatt gaagaaatcg
    16921 ctgcacaaaa agctggaatc atcaaacaag gcgttccagt agtaacagga aatattgccc
    16981 cagaagcttt tactgtcatc caacaaacag ctaaagcaaa aaatgcaccg ctttatgtat
    17041 acggggaaaa ttataaaata aactatctgc atcctgatga gatgtgggga gaagtatttg
    17101 aattttttaa tgaaatagga aagatccctc aattaaccac tccgctttta gggcaacatc
    17161 aagctgaaaa tgcaggagta gccatcgaat tgtattttgt ttactgtcaa ttggaaaagc
    17221 tgcctttccg agaaaaagat gttttgaagg gattgaagaa atctcgatgg ccaggaagaa
    17281 tggagcgttt gagtaacgag ccattagttg ttttagatgg tgcccataat acgcatgcaa
    17341 tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg atatcaaatc aacattttgt
    17401 tttccgcctt acgaactaaa aatgttagag aaatgctaga acgattgcag caagtaccaa
    17461 atgtccatat ttatttgacg acctttgaac atccaaaagc catcgaatta agtgaagtat
    17521 cagatttagc aagtgagaga gtatcaatcg tttcattgtg gcaattcgga ttagcagata
    17581 tattggaaaa gatgacaaat gaagacgtat tagttgtgac cggttcactt tatttcattt
    17641 cagaagttcg ccatttatta atacaattag gaggagatca tgaagtataa tgcagtgatt
    17701 tttgacatgg atggtttgct gttcgacaca gagttagtgt attacgaagc cacacaagta
    17761 gtagcagaca agatgggttt tccttatgat aaagacctgt atttaaaatt cttaggtgtt
    17821 tcagatgaag aagtatgggc gaattatcat atgattttct cggaatttgg caaaacaagt
    17881 gtacaaagat tcatagatga ttcataccag gaaacgcttg aacgtttttc gcaaggagcg
    17941 gtacaaatga agcccggtgt gttggaactc ctctcttttt tagaagaaca tcagattccc
    18001 aaagttgtcg cttccagtaa ccaacgagca gtcattgaac ttttattgaa gaagaacaat
    18061 ctgtttgaac gatttgagac gattgtttct gcagaaaatg ttcaacgagc aaaaccagac
    18121 ccagaaattt tcttgttagc taatcagaag ctaggtacag cgaagaaaga aacattgatt
    18181 ttagaagatt cacaaaatgg tattttagca gcttctgcgg ctgagatccc ggtagtcatg
    18241 gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa aaacagctgg ggttgtttct
    18301 tctttacatg aaatccctgc attgttaaaa taaaaatcca tctcttttcg tatcttattt
    18361 tagataagat agaaaggaga tgtttttttg ggaaaacgat tgatcaaaga agtacctact
    18421 agctctttac caagggaacg tatggaaatt tatggcgcgg aagctttatc ggatcaggag
    18481 cttttggcaa tcttactgcg taccggtcaa cacccttata gtgtgatgtc gattgctgga
    18541 aatctgttaa aaacgtttgg aggactggcc tctttacgac aggctacttt gcatgagtta
    18601 gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa tcaaagcctt gatcgaatta
    18661 ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac cggttcgtac cagctacgaa
    18721 ttggcccagc aattgattct ggaaatgaaa gatcacaaac aagaacattt ggtttgtatt
    18781 tatttggaca cgaagaatca agtgatctta aaaaaaacag tgttcatcgg ttcgctgaat
    18841 cagtcagtcg ctcatcctag agaaattttc cattatgctg tacgttattg tgcagcaagg
    18901 atcgttttag cgcataatca tccttctggt aatgttattc catcgcagca ggatatgaat
    18961 tttaccaaaa ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg
    19021 atcatcggac gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa
    19081 gttgctttct tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga
    19141 atagaagttg aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt
    19201 agtgcttttg gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg
    19261 ctgaaaaagg atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct
    19321 cagctatcca aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg
    19381 ttgaagaatc agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt
    19441 aaatcaaatt gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac
    19501 ctgccacaag agacaatttc tcttgtggca ggtatttttt tgtatgaaaa agtaaataaa
    19561 tttaagaggc tatgaaagag ccaccttgta tcaaggaata agaacaaaca aaaatggctt
    19621 ctcatgtttt tcatttgttt tctcttatat tcaggacaag gtgctgattt cataacctct
    19681 tatttattct tcatctggtg tcattaccat tggcaaaatc attggatgtc tttctgtacg
    19741 ttcgaataag aaaggctgaa gcgcgccagt catcgcttct ttcaatttat attctgaaca
    19801 gtcaggtcca ttcaaacatt ctcttaaagc gtggaataag attcgctgag cttcattgat
    19861 catttctcct gattctcgca tgtagacaaa tccacgagag agaatatctg gaccagccaa
    19921 gatttctttt ttcttgatat ccactgtcgc gacagctaat acaagacctt cttctgaaag
    19981 caggtgacgg tcacgtaaaa cgacattacc aatatcaccg atcccgctac catcgatata
    20041 cacatctcca gccacaaaat gcccagcagg acgcgcagaa tcgctagtta acgctaaaac
    20101 atcgccattt tccatgatga agcagttttc tgcaggaaca cctacatctt gtgctagaga
    20161 agcatgtact ttcaacatgc ggaattctcc gtggacagga ataaaatatt ttggcttcat
    20221 caaacgaagc attaattttt gttcttcttg tccaccgtga ccggaagtat ggatgttatt
    20281 gatttttccg tgaatcactt ctgcaccagc ttcagacagc aaattgatca aatgattgac
    20341 acttgttgta ttaccaggaa taggtgaact tgaaaagatc actgtatcgt ctggctggat
    20401 ttggatttga cggtgagtac cattcgcgat gcggctcaac gcggccatcg cttctccttg
    20461 agaacctgta cataggatca tcgtttcatt ggcaggcaag cgattgattt cgtgtgcatc
    20521 tacaaatgtg ccgtctggaa ctttgatata tcctaaacgt tgaccgttga cgatggcatt
    20581 ttccatgctt cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg catctgctgc
    20641 ttgctgcaaa cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc cttcgatttt
    20701 tgaaaagatc ttcaaaatag aatgcccaat cgttttttct gatttagtaa atgttggaat
    20761 ctcagcattt gtactatcag ataaaaggca caagactcct tcttccccga tacgagccat
    20821 tttatgcaag tttgctggtt cgccaacagg agtgaaatcg aatttgaagt ctccagtcgc
    20881 tacgatattt ccagaaggtg ttttcaccac gatccctaat gcatctggga tactatgggt
    20941 cgttctgaaa aagctgacag aagtctttct aaaacggatg atcgtatctt cattgatctc
    21001 atggagctct gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga tcaacgctaa
    21061 tgcgagagga ccagcataga taggaatatt tgcttgtttc agcaagtagg gtattccgcc
    21121 gatgtggtct tcgtgcccgt gagtaatcac caaggctttt actttgtgta agttttgtat
    21181 gatgtagctg tagtcgggaa tgacatagtc aattccaagt aaatcgtctt ccgggaattt
    21241 gatcccggca tcaatcaaaa tgatttcatc ttggaattgt accccatacg tatttttccc
    21301 gatttcacct aatccaccaa tagcaaagac gcctgtctcg ttatttttga tggaaagttt
    21361 catattaaaa ctccactaat gagaaatcag cattttcttt ttcgtactct agatgacttc
    21421 cttctagaag ttgaacgaat tcaatattgt atggggtatt ttcttccaca cgttggcgag
    21481 cgtctacatc gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt ttagggttgc
    21541 gtacttttgt ttcttgataa taaactttat agatcattgt tttgtctcct ttatctgcgt
    21601 aaagcagtga tttagctagt tgattttttt agacaacaca aaacgagggg acaagccacc
    21661 tggtttttca tatgctttga ctagaatact ggattcgagt caaagagaca agcctcgttt
    21721 ttgcttatct gttcggatat tttttcaacc gtaccttctg tataaaaaat atacaacaat
    21781 taattgttat tctagcataa aacagcaaat aagtatagaa atcaactaga ttgaaagaaa
    21841 aaatcaatgt ttatcatccc gctataatca taatgacttt tttatgagag aagtgttcta
    21901 ttaataggct cgttgtgcta tcatttttag taatgaaaat gtaacaaaag attaaaagga
    21961 gatgagaaag tgaaactaat gtggcattat accatgcgtt ataagaaatt cttgtttttc
    22021 aatttcatat gcgtatttgg ttttattttg attgaattag gacttcctac gattttagca
    22081 cgtatgatcg atgtaggaat ccgcaatgat gattttgatt atgtgaagca acaagggata
    22141 tttatgatcg ttattactgt aatcggtatt attttgaata tcttgttagg ttattttggt
    22201 tcgaggatta cgactaatat cgtagcagat attcgagatg atttgttcga aaagatccaa
    22261 agttattcgc atcaggaata cgaaaatcta ggcgtatctt cattgatcac tcgtacaaca
    22321 aacgatgctt atcagatcat gttgttcctg caaaatattt tacgtatcgg ttttatggca
    22381 ccaatgatgt tcattgtcag tttgtacatg gtcatgcgta caagtgtgac actcagtttg
    22441 tatgtagtag gtgcattacc tttactatta ttggcagtcg tagcgattgc taaggtgtct
    22501 gaaccgttat ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat cttgcgggaa
    22561 aatctttcag gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga agaaaaacgt
    22621 tttgctgtag tcaatgagaa ttatgcttct agttcaaaaa gcttattccg tttgatggct
    22681 gttgcacaac cgggttttta tttcttattt aatatcgtaa tggtattgat catctggagc
    22741 ggggcagtgc aaatcgatgc aggcgagctt cgtgtaggag atttgattgc gtttatcgag
    22801 tatatattcc atgccttgtt ctcattcatg ttatttgcta gtgtgttcat gatgtatcca
    22861 agagcagctg tttcagctgg acgtatccaa gaagccctag atgccaaacc ttcgattgta
    22921 gaagatccaa atggtatatc tgatacaaaa actaaagggt atatcgaatt tcaaaatgta
    22981 acatttgctt atccaggcca ttcgcaagag ccagtgatac gaaatgtcag ttttacagcc
    23041 tctccaggag agaccgttgc ttttattgga agtaccggta gcggaaaatc tacgttgatc
    23101 caattgatcc ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga tggagtagac
    23161 gtgagagatt atcggctaag caagttgcga gataagattg ggtttattcc gcaaaaagcg
    23221 ctgctattta ctggaacgat tgcagataat ttgcgatacg ggaaagaaga tgctacgcaa
    23281 gaagaaatgg aacgtgcagc tgagattgct caagcttcag aattcatttc tcgaaaacca
    23341 gatggattta atgaacattt atcagaaggt ggttccaatt tttcaggtgg acaaaaacaa
    23401 cgattggcaa tagccagagc catcatacgc cgtccggaaa tctatatttt tgatgacagc
    23461 ttttctgcat tagattatca aacggatgcg aagttacgtg ctcgtctgaa aaaagaaacg
    23521 acagaatcta ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca tgcagataag
    23581 atcatcgtat taaatgaagg agaagtcgtc ggtatgggga cgcatcggga gttgttggaa
    23641 aaatgcccga tttattatga tattgccgct tctcaattat caaaggagga attagcatga
    23701 aaaaaacctt ttcttcctta aaacgtttag ctcgatatat cagaccgtat cgtgagactt
    23761 ttcttttagt gatcctattt acagttctga cagtggcttt caacgtggct ttgccatatg
    23821 ttacaggact tcctactaca gaaatcagcc gtaatattgc caatggcgaa tcaatcaatt
    23881 tttcttatat attcaaatgt ctcatttgga tcacagttgt tggaataggg tattgtattt
    23941 cgcaattact ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg catgatttga
    24001 gaagagacat agaagaaaaa atcaatcgtc ttccggtttc atatttcgat aaaaaccagc
    24061 aaggaaatat tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt gctttacaac
    24121 aagcttttat tggtatcgta aatgcgatat tgggaattac gttggctgca gcaatgatgt
    24181 tttatatcca gccgatgatg gcgctgatct ctatgatcat gattccaaca tcgatctgga
    24241 tctcaaaacg agtgatcaat gcttcgcaga aatattttca aagcatgcag aattctcttg
    24301 gtgagctaaa cggctacgtc caagaaaata tgacaggttt tagtgtattg aaagtatatg
    24361 gacgcgaaaa agaaacattg gaaggattca aaaaagttaa tcacagcttg aaatactttg
    24421 ggtttcgtgc agcattcatt tctggcttga tgatgcctct agttcaatta actgcctatg
    24481 ctacttatat tggtatggct gttctaggaa gcttttatgt tattactggg gcgatcgttg
    24541 tcggtcaact acaagcattc atccaatata tttggcaaat cagtcagcca atggggaacg
    24601 tgacacaatt gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt gtattcgaga
    24661 ttttagatga gccagaagaa aaagaaaaca aagtagatgc ttcactgcca gaaacgattt
    24721 taggagatgt gacattcgat catgtcgatt ttgcatatga tccgaagaaa ccattaatcc
    24781 aagatttgaa ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt ccgaccggtg
    24841 ccggcaagac gacattgatc aatctattga tgcgttttta tgacgtagac aaaggagcaa
    24901 taaggattga tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt tcgctatttg
    24961 gtatggtctt gcaagatgca tggctttatc aaggaacgat tgcagataat atccgcttcg
    25021 gaaagcttga tgcgacagat tatgaagtag ttgatgctgc gaaaacagca aacgtagatc
    25081 attttatccg aacgatgcca gatggatatg atatggccat caattctgag ggagacaatg
    25141 tgtctttagg acaaaaacag ttgctgacga ttgcccgagc agtggtctcc gatcctaaaa
    25201 tcttgatttt agatgaagca acaagttcag tagacacccg tttagaagcg ttgattcaaa
    25261 aagcaatgga tcgcgtgatg gagggacgga caagtttcgt catcgctcac cgtttatcga
    25321 caatccgtga tgcagatctg attctagtga tggatcaagg tcagatcatt gaaaaaggaa
    25381 cgcatgagag tttattggca caaggtggat tttatgaaaa actgtataac agccaatttg
    25441 ctgaggaagc agaataagtc atataagaat aaaacagttt aaaacttatt ttttgttctc
    25501 tattcttgat aatggtaaag taaagttatc aagaaaagtg aggacacgga gatggaacag
    25561 tttttaaatg aaaaaattac tgaagccaaa agagaagttg atcgtttaac gcacgtctgt
    25621 cagacgaagc cttttgattc agctgatttt tttgaaaatg agtttcaaat cgaacgcttg
    25681 gcagcacaaa tagaagtgta cgaattaatt ttaaaaaaag taatagaaga aaatatagat
    25741 gtacaataaa aacaggtaaa acgtcagtta ccagatactg taaatagaca tctggcctga
    25801 gcaggtttta cctgtttttt ttcttatgaa ataaatatat gctatgatac ctttattcaa
    25861 taggattagt ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg tttatagcat
    25921 caaatgccac agtgattggt gatgtgacat tgtcagaaga tgtgacgatc tggtatcaag
    25981 ctgttttacg cggggacagt aattggataa aaataggaca aagaacgaat atccaagatg
    26041 gtacaatcat ccatgtcgat catgatgcac ctgtagatat tgcggagaat gtgacggtag
    26101 gacatcagtg catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc gggatgggga
    26161 ccacgatatt gaatcatgcg gttattggtg agaatagttt gattggtgct ggatcattag
    26221 taacagaagg aaaagttatt cctcctaatg tcttggcttt tggtcgccca gctaaagtga
    26281 tccgtccttt gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag cattatgtcg
    26341 agataggtcg gatgcatgcg caaggaaaat tcaaggagca aagataggca gtagcatcca
    26401 taaaataaaa aaggatggtc agtttgagtt gaccatcctt ttttatttgg tttagataag
    26461 agagcttaaa aaagcttcct ttgaaacaga ttaatttaat gaagaactgt tttccatgac
    26521 ttcatcgatc aatccatatg cttttgcttg ttcagctgtc atatagttgt cacgatctgt
    26581 atctttttca atcacttcaa gcggttgtcc agtgcgttca gctaaaattt tgtttaagcg
    26641 ttcgcgtgtt tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt gcccttgagc
    26701 gccgccaaga ggttgatgga tcatgatctc agcatttggt aaagcaaatc gtttaccttt
    26761 tgtaccagct gtcagcaaga agcttcccat tgaagcagcc atacccataa caattgtttg
    26821 gacatctgct ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg taactgaacc
    26881 accaggagaa ttgatataaa gatagatatc tttttcagaa tcttgagcgt ctaagaaaag
    26941 caattgggcg atgatcgaat tagcaagatc atctgttact tggccgctta acatgatgat
    27001 acggtctttc aataaacgtg agtagatatc gtaagctctt tcacctcgtg aagattgttc
    27061 aatgactgta ggaattaaat tcataataga gttcctcctt gatttcgttt agaaatagtg
    27121 taccataatt tagtttctaa tacctaaaat gtagtataca tttttggtca aaaaaggtca
    27181 aataaaaaac ttaggcaaat tagtcgcata aacccgcttt atggtatgat aagcagagaa
    27241 acaaaacaag gaggaacaac agattgatag ttggtattac tggccttatc ggagtgctga
    27301 caggtgctgt agttgtctat atcggcatga tactgcaaat gcgtcacttg gaaaataaag
    27361 aacagcgtag acgtgaattg gaattgaaag tcaaagaaat cgagacgctg aatcaattaa
    27421 ataaaaagat caacgagatc ttgcaaaaac ggacaacgct gatggaagaa tatgtcagct
    27481 ttgatgcatt tgatgattgt tatatcacga ttgacgattt tgcgtatctc caatcatttt
    27541 cagcgcaaaa tagtttttat ctgccaaatt atattttaga cgagctattt aagaatatct
    27601 cccatcgaaa agtgatttta tctccagatg aaacaatgaa gattggtgga tatgcttata
    27661 aaggcggacg cgtgatttta gagaatttta cggatagtct gactgaaatg atcaatgaaa
    27721 aaaaagctca gttgaagact atgacgcgcc agccgattgg tttcttctcg aaagatgatg
    27781 tttggagctg atattttgaa gcattgccgt tgcacatgag agcgtggtaa tgctttcttt
    27841 ttttcttaat gatacaatag aactgaacgg agtgaagaaa gatgttacaa atctatgccc
    27901 atcgcggaag cagcggcacc cacccagaaa atacgcttcc tgcatttgca gaagctgtac
    27961 gagtaggtgc agatggtatc gaactagatg tccatctaag caaagatggt tatttgatcg
    28021 ttatgcacga cgaagaagtg gaccggacga ctaacggtaa agggctgatt cgagaaaaaa
    28081 cattggaaga actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa tttccagcag
    28141 ctaaagttcc tgaattgaga gaagtgttag gactgctcct ccaaaaaaat taccgaggga
    28201 tgctaatgat tgaaattaaa actgatcagt atgaatatcc cggtatcgaa gaaaaattag
    28261 ccgaaacatt aaaaagtaga gattggcctt ttcaacatgc atattgcagc tttcatttgg
    28321 attctttgga aaaaatcgca ttactggaac cggagataca gctggactta ttgatgagta
    28381 cttctgataa aaagatccaa ctagcagcag aaacccctta tatagaagga gtacatccaa
    28441 aaaatacttg gttgttcgaa catccagtcc aggcagctga ttttccaaaa gcaattcgtg
    28501 catggacgat taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa atagatggaa
    28561 taataactga ttttccggag aaagctcttc ggtggaaaca atcaacgaaa agtaggtaaa
    28621 atatggaaaa agtattagtg atcgtcggtc cgacagcagt cggtaaaaca gcgctaagtg
    28681 tggaactcgc acaactattt ggaggagaga tcatcagtgg tgattcattg caaatctata
    28741 aaaatctgga tatcggtaca gcaaaaatca aagaagaaga gatgaataat gttccgcatc
    28801 atttgattga tgtgatcgaa cctactcaac gttactcagc cgttgatttt cagcattcag
    28861 gtcgacaatt gattaccgag attgcgaacc gagggcacct acctatcata gctggtggta
    28921 cgggtctcta tatccaatcc cttctatatg attatcagtt aggagcgaca gaagaagcga
    28981 atcctgagat tagaaaaaag tatgaaacgt tagctgaaac aattggttcc caagcattat
    29041 ggaatcagtt gaaagaaaaa gacccggcag ctgctgagaa aatccactgg aacaatcaac
    29101 gcaaagtgat aagagctctg gaagttttag agacgacagg acatagtatc acggcaccta
    29161 aagaagagcc aaagcaattg tatgattacc tcatgattgg attagatacg aagcgtagta
    29221 ttctttatga acgaatcaat caaagagtcg atttgatgct agcagaaggt ttagtagcag
    29281 aagcgcgtaa ggtgtatgag cttggagaag tccaggccag tcaaggtatt ggatataaag
    29341 aatttttccc ttattttgaa gggaaagaaa cattggaaga agcaacagaa caagtcaaac
    29401 tacattcgag aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt ttggatgcac
    29461 agtggtttga tcttataagt gagccaaatc aaaaaagtca gattgagcaa acaatcaaaa
    29521 aatggctgga ggcggaataa aatggaagaa aaagtgatca tcgtaggggt agaaaccgaa
    29581 gcaaaccagc gttacttttc agagtcaatg gaagagctag ttcagctgac gaataccgca
    29641 tcaggagaag tcgtattcac aattacacaa aagagaccac aagtggatcg gcaaacgatt
    29701 atcggtaaag ggaaattaca agaattagtt caacaagcgg atgcgcatga agcggatttg
    29761 atcatcttta atcatgagct aactccgaga cagagccaac tgattaccga tgctgttggt
    29821 cttccggtaa ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg tgctcgttcg
    29881 aaagaaggga agctgcaggt tgaacttgct cagttagaat acctgctgcc tcgtttagta
    29941 gggcaaggga aaacattatc tcgtcttggc ggtgggatcg gaacaagagg gccaggtgaa
    30001 acaaagttag aaacagaccg ccgacatata cggaataaaa tttttgctgt taagaaagaa
    30061 ttgaaagaag tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa tagcgagatt
    30121 tttcagattg gacttattgg ctacacgaat gccgggaagt caactatctt gaatctattg
    30181 acacaagcgg atacttattc gaaagatcaa ttgtttgcaa cgttagatcc attgactaag
    30241 agatggcgtt ttgctgaagg atttgagatt actgtaacgg ataccgtcgg gttcatccaa
    30301 gacctgccta cgcaactgat cgatgctttc cattctacat tagaagaaag tcaaaatatg
    30361 gatcttttgc tacatgtagt cgatgctagt tctccagatc gtattttaca ggagcagact
    30421 gtcttaaaat tgatggatga actgaatatg aaagaaatgc cgatactgac tgtttacaat
    30481 aaggcagacc agatcgaccc agcgatgttc acgcctacgc tattcccaaa tgtattgatt
    30541 tctactcaga cgaaagaagg taagatggcg ttgatcgaag caatcaaacg tcagctgatg
    30601 gaactaatga ccccttatac actttctgtg ccaagcaacg aaggacagaa attaagcgag
    30661 ttgagaagac aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa cgaatatgaa
    30721 gttaaagggt ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata aaattttatt
    30781 tttttaa
//
LOCUS       DANKBF010000029        30460 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000029 DANKBF010000000
VERSION     DANKBF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(451..1761)
                     /locus_tag="PWY89_001428"
     CDS             complement(451..1761)
                     /locus_tag="PWY89_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2066472.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="PWY89_001429"
     CDS             complement(1773..3131)
                     /locus_tag="PWY89_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2066473.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3333..4901)
                     /locus_tag="PWY89_001430"
     CDS             complement(3333..4901)
                     /locus_tag="PWY89_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2066474.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4905..5315)
                     /locus_tag="PWY89_001431"
     CDS             complement(4905..5315)
                     /locus_tag="PWY89_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2066475.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5312..6235)
                     /locus_tag="PWY89_001432"
     CDS             complement(5312..6235)
                     /locus_tag="PWY89_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL2066476.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6232..7110)
                     /locus_tag="PWY89_001433"
     CDS             complement(6232..7110)
                     /locus_tag="PWY89_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL2066477.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7097..8434)
                     /locus_tag="PWY89_001434"
     CDS             complement(7097..8434)
                     /locus_tag="PWY89_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2066478.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8437..9540)
                     /locus_tag="PWY89_001435"
     CDS             complement(8437..9540)
                     /locus_tag="PWY89_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2066479.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9614..11122)
                     /locus_tag="PWY89_001436"
     CDS             complement(9614..11122)
                     /locus_tag="PWY89_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2066480.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11088..11780)
                     /locus_tag="PWY89_001437"
     CDS             complement(11088..11780)
                     /locus_tag="PWY89_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2066481.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11802..12776)
                     /locus_tag="PWY89_001438"
     CDS             complement(11802..12776)
                     /locus_tag="PWY89_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2066482.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13076..13948
                     /locus_tag="PWY89_001439"
     CDS             13076..13948
                     /locus_tag="PWY89_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2066483.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14406..16604)
                     /locus_tag="PWY89_001440"
     CDS             complement(14406..16604)
                     /locus_tag="PWY89_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066484.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16836..17828
                     /locus_tag="PWY89_001441"
     CDS             16836..17828
                     /locus_tag="PWY89_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2066485.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17888..19309
                     /locus_tag="PWY89_001442"
     CDS             17888..19309
                     /locus_tag="PWY89_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL2066486.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19302..20801
                     /locus_tag="PWY89_001443"
     CDS             19302..20801
                     /locus_tag="PWY89_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL2066487.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20840..22234
                     /gene="uxaC"
                     /locus_tag="PWY89_001444"
     CDS             20840..22234
                     /gene="uxaC"
                     /locus_tag="PWY89_001444"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2066488.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22393..23076
                     /locus_tag="PWY89_001445"
     CDS             22393..23076
                     /locus_tag="PWY89_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL2066489.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23078..23914
                     /locus_tag="PWY89_001446"
     CDS             23078..23914
                     /locus_tag="PWY89_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2066490.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23925..25679
                     /locus_tag="PWY89_001447"
     CDS             23925..25679
                     /locus_tag="PWY89_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066491.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25955..26296)
                     /locus_tag="PWY89_001448"
     CDS             complement(25955..26296)
                     /locus_tag="PWY89_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2066492.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26364..28535)
                     /locus_tag="PWY89_001449"
     CDS             complement(26364..28535)
                     /locus_tag="PWY89_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2066493.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28704..29603
                     /locus_tag="PWY89_001450"
     CDS             28704..29603
                     /locus_tag="PWY89_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2066494.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29674..>30460)
                     /locus_tag="PWY89_001451"
     CDS             complement(29674..>30460)
                     /locus_tag="PWY89_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2066495.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9099 a         6274 c         5391 g         9696 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3301 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3361 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3421 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3481 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3541 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3601 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3661 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3721 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3781 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3841 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3901 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3961 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     4021 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4081 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4141 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4201 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4261 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4321 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4381 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4441 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4501 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4561 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4621 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4681 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4741 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4801 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4861 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4921 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4981 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     5041 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5101 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5161 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5221 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5281 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5341 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5401 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5461 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5521 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5581 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5641 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5701 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5761 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5821 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5881 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5941 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     6001 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6061 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6121 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6181 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6241 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6301 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6361 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6421 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6481 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6541 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6601 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6661 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6721 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6781 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6841 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6901 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6961 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     7021 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7081 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7141 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7201 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7261 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7321 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7381 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7441 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7501 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7561 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7621 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7681 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7741 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7801 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7861 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7921 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     7981 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     8041 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8101 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8161 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8221 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8281 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8341 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8401 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8461 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8521 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8581 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8641 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8701 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8761 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8821 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8881 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8941 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     9001 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9061 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9121 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9181 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9241 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9301 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9361 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9421 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9481 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9541 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9601 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9661 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9721 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9781 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9841 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9901 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9961 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
    10021 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10081 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10141 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10201 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10261 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10321 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10381 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10441 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10501 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10561 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10621 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10681 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10741 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10801 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10861 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10921 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10981 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    11041 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11101 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11161 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11221 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11281 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11341 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11401 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11461 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11521 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11581 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11641 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11701 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11761 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11821 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11881 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11941 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    12001 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12061 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12121 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12181 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12241 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12301 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12361 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12421 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12481 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12541 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12601 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12661 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12721 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12781 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12841 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12901 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12961 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    13021 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13081 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13141 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13201 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13261 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13321 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13381 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13441 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13501 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13561 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13621 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13681 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13741 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13801 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13861 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13921 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13981 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    14041 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14101 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14161 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14221 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14281 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14341 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14401 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14461 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14521 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14581 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14641 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14701 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14761 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14821 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14881 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14941 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    15001 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15061 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15121 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15181 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15241 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15301 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15361 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15421 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15481 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15541 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15601 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15661 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15721 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15781 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15841 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15901 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15961 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    16021 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16081 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16141 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    16201 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16261 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16321 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16381 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16441 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16501 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16561 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16621 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16681 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16741 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16801 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    16861 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16921 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16981 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    17041 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17101 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17161 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17221 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17281 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17341 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17401 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17461 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17521 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17581 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17641 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17701 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17761 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17821 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17881 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17941 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    18001 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18061 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18121 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18181 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18241 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18301 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18361 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18421 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18481 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18541 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18601 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18661 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18721 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18781 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18841 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18901 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18961 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    19021 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19081 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19141 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19201 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19261 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19321 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19381 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19441 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19501 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19561 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19621 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19681 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19741 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19801 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19861 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19921 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19981 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    20041 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20101 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20161 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20221 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20281 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20341 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20401 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20461 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20521 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20581 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20641 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20701 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20761 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20821 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20881 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20941 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    21001 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21061 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21121 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21181 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21241 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21301 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21361 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21421 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21481 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21541 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21601 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21661 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21721 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21781 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21841 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21901 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21961 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    22021 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22081 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22141 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22201 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22261 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22321 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22381 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22441 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22501 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22561 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22621 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22681 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22741 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22801 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22861 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22921 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22981 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    23041 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23101 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23161 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23221 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23281 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23341 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23401 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23461 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23521 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23581 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23641 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23701 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23761 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23821 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23881 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23941 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    24001 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24061 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24121 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24181 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24241 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24301 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24361 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24421 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24481 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24541 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24601 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24661 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24721 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24781 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24841 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24901 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24961 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    25021 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25081 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25141 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25201 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25261 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25321 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25381 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25441 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    25501 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25561 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25621 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25681 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    25741 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    25801 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    25861 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    25921 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    25981 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    26041 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    26101 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    26161 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    26221 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    26281 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    26341 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    26401 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    26461 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    26521 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    26581 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    26641 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    26701 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    26761 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    26821 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    26881 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    26941 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    27001 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    27061 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    27121 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    27181 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    27241 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    27301 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    27361 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    27421 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    27481 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    27541 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    27601 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    27661 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    27721 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    27781 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    27841 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    27901 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    27961 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    28021 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    28081 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    28141 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    28201 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    28261 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    28321 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    28381 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    28441 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    28501 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    28561 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    28621 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    28681 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    28741 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    28801 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    28861 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    28921 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    28981 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    29041 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    29101 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    29161 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    29221 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    29281 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    29341 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    29401 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    29461 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    29521 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    29581 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    29641 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    29701 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    29761 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    29821 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    29881 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    29941 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    30001 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    30061 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    30121 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    30181 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    30241 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    30301 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    30361 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    30421 caatgtttct tctggtgttg tggtagggat acttaatttt
//
LOCUS       DANKBF010000030        29756 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000030 DANKBF010000000
VERSION     DANKBF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..257
                     /locus_tag="PWY89_001452"
     CDS             <1..257
                     /locus_tag="PWY89_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2066496.1"
                     /translation="LDADSIVLDTRNTLDYTKNHLSHSLSIPAAILPRQLKNLDPKKL
                     YYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            429..1049
                     /locus_tag="PWY89_001453"
     CDS             429..1049
                     /locus_tag="PWY89_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL2066497.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(1141..2307)
                     /locus_tag="PWY89_001454"
     CDS             complement(1141..2307)
                     /locus_tag="PWY89_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2066498.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(2319..2672)
                     /locus_tag="PWY89_001455"
     CDS             complement(2319..2672)
                     /locus_tag="PWY89_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2066499.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            2800..3822
                     /locus_tag="PWY89_001456"
     CDS             2800..3822
                     /locus_tag="PWY89_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2066500.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            3841..4002
                     /locus_tag="PWY89_001457"
     CDS             3841..4002
                     /locus_tag="PWY89_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066501.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(4261..4695)
                     /locus_tag="PWY89_001458"
     CDS             complement(4261..4695)
                     /locus_tag="PWY89_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066502.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(4805..6664)
                     /locus_tag="PWY89_001459"
     CDS             complement(4805..6664)
                     /locus_tag="PWY89_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2066503.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(6782..7942)
                     /locus_tag="PWY89_001460"
     CDS             complement(6782..7942)
                     /locus_tag="PWY89_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL2066504.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(8249..9673)
                     /gene="araA"
                     /locus_tag="PWY89_001461"
     CDS             complement(8249..9673)
                     /gene="araA"
                     /locus_tag="PWY89_001461"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL2066505.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(9689..10387)
                     /locus_tag="PWY89_001462"
     CDS             complement(9689..10387)
                     /locus_tag="PWY89_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2066506.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(10344..12005)
                     /locus_tag="PWY89_001463"
     CDS             complement(10344..12005)
                     /locus_tag="PWY89_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2066507.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12223..13623
                     /locus_tag="PWY89_001464"
     CDS             12223..13623
                     /locus_tag="PWY89_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2066508.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(13875..14954)
                     /locus_tag="PWY89_001465"
     CDS             complement(13875..14954)
                     /locus_tag="PWY89_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2066509.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(15040..15999)
                     /locus_tag="PWY89_001466"
     CDS             complement(15040..15999)
                     /locus_tag="PWY89_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2066510.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(16011..17522)
                     /locus_tag="PWY89_001467"
     CDS             complement(16011..17522)
                     /locus_tag="PWY89_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2066511.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(17544..18107)
                     /locus_tag="PWY89_001468"
     CDS             complement(17544..18107)
                     /locus_tag="PWY89_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2066512.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(18113..18976)
                     /locus_tag="PWY89_001469"
     CDS             complement(18113..18976)
                     /locus_tag="PWY89_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2066513.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(18990..19886)
                     /locus_tag="PWY89_001470"
     CDS             complement(18990..19886)
                     /locus_tag="PWY89_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2066514.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(20061..21350)
                     /locus_tag="PWY89_001471"
     CDS             complement(20061..21350)
                     /locus_tag="PWY89_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2066515.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(21322..22839)
                     /locus_tag="PWY89_001472"
     CDS             complement(21322..22839)
                     /locus_tag="PWY89_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2066516.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            23037..23927
                     /locus_tag="PWY89_001473"
     CDS             23037..23927
                     /locus_tag="PWY89_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL2066517.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(24000..24824)
                     /gene="nadE"
                     /locus_tag="PWY89_001474"
     CDS             complement(24000..24824)
                     /gene="nadE"
                     /locus_tag="PWY89_001474"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL2066518.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(24836..26308)
                     /locus_tag="PWY89_001475"
     CDS             complement(24836..26308)
                     /locus_tag="PWY89_001475"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2066519.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(26945..27307)
                     /locus_tag="PWY89_001476"
     CDS             complement(26945..27307)
                     /locus_tag="PWY89_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2066520.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            27530..28282
                     /locus_tag="PWY89_001477"
     CDS             27530..28282
                     /locus_tag="PWY89_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2066521.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(28422..29345)
                     /locus_tag="PWY89_001478"
     CDS             complement(28422..29345)
                     /locus_tag="PWY89_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2066522.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(29360..29632)
                     /locus_tag="PWY89_001479"
     CDS             complement(29360..29632)
                     /locus_tag="PWY89_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066523.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         8831 a         6193 c         5086 g         9646 t
ORIGIN      
        1 ctctcgatgc tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc
       61 acttaagcca ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag
      121 atccaaagaa actttattat attctaagct atacaggcag gcgatcagaa gcattagccg
      181 atcaacttac tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc
      241 agtcgagtgt tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt
      301 gtcacagcct cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata
      361 tgacttgata gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag
      421 gtaagcatat ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc
      481 gcacagttaa ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg
      541 aagtgactgt tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat
      601 caggttggga agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac
      661 aaaaaattgc tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta
      721 tcgcaaacgc cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg
      781 tcaatgtagc tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag
      841 gtaaaaaagc actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct
      901 ctcaatatgt aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca
      961 tcgaaggaat cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata
     1021 aagctgctgc tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag
     1081 aggaaaaaat cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg
     1141 ttattctttc tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca
     1201 ttcaaaaaca tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac
     1261 accgatgatt ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg
     1321 gattgctaac tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc
     1381 tactgcaaaa acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa
     1441 gatagccgga atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc
     1501 aataatcccc aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat
     1561 gattgccaca gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt
     1621 catttcactc agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa
     1681 ataaggtgct aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag
     1741 cagagccact aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact
     1801 cttagatata tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc
     1861 caactgttcg cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc
     1921 aatcgtatct acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt
     1981 agggatgatc actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat
     2041 gatactgtaa agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa
     2101 gtaatacaag attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat
     2161 tggtgcaaac aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac
     2221 aacaagtaag gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg
     2281 tctgttcttc ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt
     2341 cccaaaccaa tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct
     2401 tcttctggac aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg
     2461 gccatcggac cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct
     2521 gctttcaaag aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag
     2581 gcagctgttg ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct
     2641 gtcccaattt ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa
     2701 aacagtataa cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa
     2761 cgaaaactat ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta
     2821 ggaacctata cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa
     2881 gaagaattag ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa
     2941 agtaaagctg gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg
     3001 tacgataagg attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat
     3061 gtagcggttg atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta
     3121 aatgtctacc atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa
     3181 gaagcgacag gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact
     3241 cctgaccaac gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg
     3301 tccgaaaacg gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct
     3361 cgccatcttg tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc
     3421 acagtccgcg tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata
     3481 tcgaccttgc cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat
     3541 gatggcaagt tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt
     3601 tttgaagatg gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc
     3661 cgcgatttcg cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat
     3721 aatctgacat tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat
     3781 gtctatgtac cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa
     3841 atgaatcagc aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat
     3901 gggaaaatcg aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta
     3961 cagaactact ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc
     4021 tgaaaagaac atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa
     4081 aaaccgccga gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta
     4141 caaggttgtt gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat
     4201 aaaggtaata ttacatttat attagataac tctagttttt attgttatca aagatttctt
     4261 ttattttttc aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc
     4321 ttggtcttgt tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc
     4381 gatactttgg atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt
     4441 tttatgaatg cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc
     4501 ccgatatttt tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc
     4561 cagttcactt gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat
     4621 tccttctccg attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc
     4681 aatattcggt aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact
     4741 tgtttttatc ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc
     4801 attcttattc gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt
     4861 catattggat caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt
     4921 ctttatacca ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt
     4981 ctaatgataa aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat
     5041 tcgctgcaaa ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc
     5101 ctttactgtc gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt
     5161 ggatcgtatc tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc
     5221 tagaaagatt cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat
     5281 cttgcagata attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt
     5341 ctagattatc atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga
     5401 actgaggtgt tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag
     5461 aaatatatgg attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact
     5521 ggtcaaatcc taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat
     5581 ggatcgctgt cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat
     5641 ccatttttgg tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag
     5701 aaaaaccagt caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa
     5761 gcatattgcc actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag
     5821 atacttctac gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga
     5881 tgttcggcga ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt
     5941 cagtgatttc tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct
     6001 tcagattgta caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt
     6061 aaggtatcca tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga
     6121 aattactgat ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt
     6181 ggaatcgcct tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg
     6241 ttcctgcggc agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc
     6301 cgatttcagt gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt
     6361 tcatataatt tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag
     6421 ggacagatcc tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta
     6481 agaagaattt ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat
     6541 cattttgaca ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga
     6601 ttgctgctgt tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt
     6661 tcaaatgctt gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg
     6721 tacgaaattc gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc
     6781 tttatggcct ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta
     6841 gctgttgttt cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt
     6901 agacatgctc attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca
     6961 gcagagaaaa tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa
     7021 acggcgcttg gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga
     7081 acaaaagata atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct
     7141 gagtcgatcc ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt
     7201 ctggaaaatc agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat
     7261 atttatcaac aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt
     7321 ctatattcgc ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga
     7381 tgtaatccga ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg
     7441 catcgcggat agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt
     7501 ttgtgcctat cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca
     7561 cataagcact tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta
     7621 ccatacgact tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct
     7681 cttttactgc attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag
     7741 ctttttcaac gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg
     7801 gtgtaagcca ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat
     7861 catctattat tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat
     7921 caatcaaatg aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct
     7981 gcaattagac aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga
     8041 gtaggctgag tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag
     8101 agcaaaatat tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa
     8161 taagactaaa tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa
     8221 aactaagtgg ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta
     8281 attttgccca cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac
     8341 cgccacctgc ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata
     8401 acacacgtgc aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat
     8461 tgatcagcag tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg
     8521 ctttcccatc aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa
     8581 caacgattct tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca
     8641 agattgcttc ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt
     8701 tccctcgagc catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc
     8761 cagcaaaacc gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca
     8821 ttccatacag gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac
     8881 ggcgaatacc gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt
     8941 cataatcacc atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt
     9001 cttcgtctga tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag
     9061 tccaaccaaa ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca
     9121 tgttgtcacc aaaacgagcc acttttattc caaaactttc attataggct actgcaacat
     9181 ccatccaatc agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt
     9241 tattttgttt atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt
     9301 gatttaaatt catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg
     9361 tagctaaatg aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg
     9421 gtgagaaagt atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt
     9481 tcatgatttt tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag
     9541 gcaattggcc attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc
     9601 gcagtgcttc ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc
     9661 caatattcaa cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc
     9721 cgtgttttcg taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat
     9781 tgagctgtct ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta
     9841 ccgcactttg cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg
     9901 gaattgctaa gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt
     9961 catattcgta cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc
    10021 cataaaaagt atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc
    10081 agatcgtcgc ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat
    10141 ataaaacagc atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat
    10201 tgtccaagtc cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag
    10261 gtttgataac gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt
    10321 tgacgagtcc gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt
    10381 ccagcatttt ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg
    10441 atcccctcac ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt
    10501 gtgatggtga ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac
    10561 ttcccatgtc gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct
    10621 ccggctgtgt ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca
    10681 acttctggtg ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca
    10741 gatttcaata tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc
    10801 atgaaattcg ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca
    10861 tttactcctg taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc
    10921 gtatcgcctt ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc
    10981 atacccaaag aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag
    11041 cagttattcg tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt
    11101 tccggatgga cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca
    11161 gccgaaatgt tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca
    11221 tccccttctg gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga
    11281 taagcccctg attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa
    11341 atgtgatcca agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc
    11401 atttcttgat ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct
    11461 acgcctagta ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa
    11521 aaattaattt ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata
    11581 ctccatctct gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca
    11641 gtgattgtat ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct
    11701 agataaccgt gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca
    11761 tagatttcga atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata
    11821 tcttctaatg aataggtcca gataccattt tctagtttgt tttcccactc ataactacca
    11881 gcggcaatag gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac
    11941 tcaatcccta aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc
    12001 ttcataatga tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc
    12061 gcttacaatg attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata
    12121 caataaaaaa cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc
    12181 tgatcaaccg ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa
    12241 gtttcctcat cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat
    12301 attggtgtta tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa
    12361 gcttctttga taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct
    12421 ttatctggtc ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt
    12481 atttttatgg ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg
    12541 attgcagcaa ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct
    12601 tatatgtctg aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg
    12661 atgattgctt ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct
    12721 gaaacgatgg cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt
    12781 ttcggtgttt tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa
    12841 gaggcaaaaa gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa
    12901 caaatccagc tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt
    12961 ttagaaaaat atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt
    13021 caaggagcta atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat
    13081 gcagcgagcg atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct
    13141 ttgctctttt tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt
    13201 agcgtgatgg gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat
    13261 acaaattcgt tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca
    13321 tgggctccac tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga
    13381 gcatctggcc tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt
    13441 cctgtgatga ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc
    13501 tttttagggg ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag
    13561 gaaatcgaac aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct
    13621 taaagaggtt gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc
    13681 agtcacaagg aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg
    13741 accagttggt aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga
    13801 gcgatgacaa aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg
    13861 taaaaacccg tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt
    13921 tcatagagaa tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg
    13981 gcatcttgtc ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa
    14041 tggcttaaag aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca
    14101 ttttctgtca acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca
    14161 tctaaagctt gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt
    14221 tccgtggtga aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg
    14281 ctgataaaac ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac
    14341 agacgctgat gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca
    14401 tctaaacaaa tataagaggc atttaactct tcataagcag cattgatcat caccatcgga
    14461 atgccttgtt ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc
    14521 gtaggctcaa cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc
    14581 tccaattcgt gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat
    14641 tcattttcga ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg
    14701 acaccgatcg tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca
    14761 tatgtgcccg accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct
    14821 tgccgaatcg tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg
    14881 gttgtattta cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct
    14941 tgatattttg ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc
    15001 tgcaaggttt attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg
    15061 ttcaccaaat tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg
    15121 attaggattg tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa
    15181 aatatcaaag cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt
    15241 atttttttca gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa
    15301 aagattcttg tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact
    15361 accagaatac gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac
    15421 tgaaaaaccc cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt
    15481 taacgtccaa ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg
    15541 atcttgttgg gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa
    15601 actgaaagtt tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg
    15661 atctatatgc tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt
    15721 tttatgattg cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg
    15781 tgcccagatc aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc
    15841 agcatgttct aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg
    15901 aaccgatcct gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg
    15961 ttgccgaacg ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc
    16021 tttattctaa ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt
    16081 tctattgaaa aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg
    16141 tccaacagat cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca
    16201 tgttcaatcg ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga
    16261 ttgccgttat ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca
    16321 taaacagggc aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt
    16381 tgcatcacat aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa
    16441 ggtattgtgt ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa
    16501 atagccagcc ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg
    16561 atgggactac cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat
    16621 tcatcaaacg ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa
    16681 gtagcaacga tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct
    16741 gtatcatcac gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata
    16801 tagttatgca aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc
    16861 cattcagggt acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa
    16921 tcatccacta acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca
    16981 ctcttagccc ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg
    17041 ccaaaaggct ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga
    17101 aaattacaat attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc
    17161 gtgaatatcg gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa
    17221 gaatttggtt ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta
    17281 cctacagtat ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca
    17341 cctaattcta attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct
    17401 gtttcatggc ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat
    17461 gagccaaaca gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc
    17521 acttcttcgt ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca
    17581 agtttccata cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt
    17641 acaaatccta ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc
    17701 catgcacttt gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat
    17761 atccaggata aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt
    17821 acgattgtct gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga
    17881 ctaaggaaca agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata
    17941 tttcctgatc cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc
    18001 acgatagtaa taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca
    18061 gaaaccacaa tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt
    18121 tcattccaga catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta
    18181 gtaatggcag cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct
    18241 gccctgtaaa taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata
    18301 tcaatggata aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa
    18361 gagtaggttt gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat
    18421 caatgcgtgc tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga
    18481 agataccaaa tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc
    18541 caagcaaatt gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg
    18601 tcagcaaaac gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat
    18661 accccgcaag agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg
    18721 tatttttgat gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg
    18781 tatagctatc tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag
    18841 aagaaataat cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa
    18901 tcgttcgaat gattttttta ccactaactg cttttcgact attcacagta actgtaatcg
    18961 agctttctgg tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg
    19021 tacacattag tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct
    19081 gatgcatagc ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct
    19141 gtttctgtct taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc
    19201 atcgaaccaa tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc
    19261 gtaatgttaa aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa
    19321 gagggagaga ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc
    19381 caaacagtca tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg
    19441 atcggcaagc cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac
    19501 atagaaaaaa taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa
    19561 cgacaaaaac gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg
    19621 ataatttgtg ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact
    19681 gctcgttcat cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta
    19741 aagaaaatat tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta
    19801 aataataata aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga
    19861 ttccaatatt tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat
    19921 acaatcttgg tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa
    19981 taacacctat ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct
    20041 atcctgtata aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg
    20101 ttcatgagtg tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata
    20161 tcgttattga tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt
    20221 tgtttcgata attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa
    20281 tccaagtagg caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc
    20341 caatatttca aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg
    20401 gcatatccta cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag
    20461 gtagtcacat tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg
    20521 atttcaatgg ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat
    20581 tctggaccag tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc
    20641 tcaggagacg caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct
    20701 aacatccagt tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt
    20761 ttactactat cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt
    20821 tgttcagtat ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct
    20881 tgtacctgca tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt
    20941 tctttaaaat cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc
    21001 gctccgtttt ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct
    21061 tttttagaag aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc
    21121 atttcaattt tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat
    21181 atttcaccat cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa
    21241 tcagattctg ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac
    21301 aaagagccaa ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg
    21361 acatctgacc acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc
    21421 ttttacattt gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt
    21481 tgtatcaaat ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa
    21541 gtgaacagtt agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt
    21601 gtcatttatt acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata
    21661 gtttgctgaa cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat
    21721 aaatggataa tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc
    21781 aataacgttt accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa
    21841 agtgattaat agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa
    21901 aggaggagca atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat
    21961 attccattca tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt
    22021 atcgcacatc gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata
    22081 atgagaaaga tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg
    22141 atcgtacgct tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt
    22201 cgaacttccg cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt
    22261 ttcctctgct aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc
    22321 catttcattc cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg
    22381 ccttaaatca ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc
    22441 tgcatctact ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg
    22501 acaccagtta taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa
    22561 atccaacctt ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga
    22621 cacaaaattt ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat
    22681 gacgtcttgg cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc
    22741 atatactgct cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc
    22801 tacacgcttg acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat
    22861 acatgaataa tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat
    22921 tttttatcga ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata
    22981 acttttatta acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc
    23041 aactacaaat aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga
    23101 tcaagatcat ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac
    23161 ttggagtgag cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca
    23221 tcaaaacaga aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata
    23281 ttagttttcc agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg
    23341 gtcactatac aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta
    23401 tcgggaaagt ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc
    23461 tctggtttac cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata
    23521 cattggagat gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca
    23581 attggccttc agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc
    23641 ctggagattt tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta
    23701 atcaatacgg cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag
    23761 aacccttgtc aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc
    23821 gaatcgaagt gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat
    23881 tcggtaatta cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc
    23941 tattttataa aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt
    24001 tatttccaga aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc
    24061 caccatgctt cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca
    24121 tcaatttcat tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct
    24181 tcaagatctg cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc
    24241 aattgtttcc cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca
    24301 ccaaatttcg tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct
    24361 cctgctcttt ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt
    24421 cctttgttaa aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca
    24481 acagctggtt tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct
    24541 tttgcatctt cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag
    24601 ttttcatctt ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct
    24661 aaagtagaat cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt
    24721 ttcaaatatg ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt
    24781 ggtttgacac ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg
    24841 cttctgtatg aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt
    24901 tccagcattc agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact
    24961 cttcccatag tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta
    25021 attcgtaaac tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt
    25081 ctcgaacagt tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt
    25141 ctcgagggtc ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc
    25201 gagtgatgcg ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata
    25261 acttgatcgt atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg
    25321 caccaagggc tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa
    25381 ttttagcttt ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat
    25441 agatttttgc ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat
    25501 aggccatatc tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt
    25561 tcgccacgcg aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga
    25621 acacgcagtc tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag
    25681 attggactaa cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac
    25741 gtacattgct tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg
    25801 catccagttc ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag
    25861 atttgatacg agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg
    25921 ctgtttctac taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg
    25981 caaaaactag ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta
    26041 aatactccaa aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat
    26101 cattgaacgt cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt
    26161 atccgtggtt aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc
    26221 gacctaattc ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca
    26281 aactgtcgtc tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt
    26341 cctacccatt ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa
    26401 gcacatgttt tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt
    26461 cttattggta tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca
    26521 cgaagaaatt agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac
    26581 aatcgtagcc gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata
    26641 atcaatggca aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca
    26701 aagaaacgga tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga
    26761 tataccttgc gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa
    26821 gtaagaaagc agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc
    26881 cttgtttttt attgtataca ttagatatgg agcaggttac cataaagatg attattagtc
    26941 gttattaaat agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac
    27001 gtttgaattg tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat
    27061 cttctcgtgt catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt
    27121 ttttcttcgt attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt
    27181 gtgttcgatc taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt
    27241 ccactacttt aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta
    27301 gcttcatctc attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac
    27361 catctttcct agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac
    27421 cttttatcac ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac
    27481 catctcgtta cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac
    27541 aataaagaaa atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga
    27601 gattggactg ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa
    27661 agaagtcgat cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga
    27721 tatccaagag gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca
    27781 gcaaatgttg ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac
    27841 acttgatatg tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa
    27901 acaacaaaag ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg
    27961 aaaaaaaaag gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga
    28021 agaaatgcaa actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc
    28081 agaagatgaa agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag
    28141 ctggcctgaa tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga
    28201 acggttcact gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga
    28261 aattatccaa aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg
    28321 acctaataac aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc
    28381 tccattcttt cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta
    28441 taaggtattt tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg
    28501 caagcttcca gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta
    28561 tcgcgtccaa tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca
    28621 accatatccg aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct
    28681 tgttttcctt tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga
    28741 aaaagttgat gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc
    28801 cccaacgtga tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta
    28861 attgattgtt ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa
    28921 aaaactagag gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac
    28981 gtaatgatca atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt
    29041 tctttctgat agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca
    29101 atgatcccac tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca
    29161 atgcttttcg tttttccata acttaactgt ctggcatttg cattagggat ataatcgact
    29221 tctttcatga ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg
    29281 attacacgtg aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt
    29341 gccactgctg cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg
    29401 gaccgcattc aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc
    29461 cccaatatat atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat
    29521 tcctattcaa cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg
    29581 cataaaaata attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc
    29641 ctccttttga tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt
    29701 gccaaagaaa agttttattc ctctttcatt attaccaaag tctcttattt taaatt
//
LOCUS       DANKBF010000031        29251 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000031 DANKBF010000000
VERSION     DANKBF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(104..1336)
                     /locus_tag="PWY89_001480"
     CDS             complement(104..1336)
                     /locus_tag="PWY89_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2066524.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="PWY89_001481"
     CDS             1591..2160
                     /locus_tag="PWY89_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2066525.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="PWY89_001482"
     CDS             complement(2338..2778)
                     /locus_tag="PWY89_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2066526.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="PWY89_001483"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="PWY89_001483"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2066527.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="PWY89_001484"
     CDS             3732..4655
                     /locus_tag="PWY89_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2066528.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="PWY89_001485"
     CDS             4731..5870
                     /locus_tag="PWY89_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2066529.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="PWY89_001486"
     CDS             5863..6663
                     /locus_tag="PWY89_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2066530.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="PWY89_001487"
     CDS             6663..7490
                     /locus_tag="PWY89_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2066531.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="PWY89_001488"
     CDS             7468..8202
                     /locus_tag="PWY89_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2066532.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="PWY89_001489"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="PWY89_001489"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2066533.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="PWY89_001490"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="PWY89_001490"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2066534.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="PWY89_001491"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="PWY89_001491"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2066535.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="PWY89_001492"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="PWY89_001492"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2066536.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="PWY89_001493"
     CDS             11787..13820
                     /locus_tag="PWY89_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066537.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="PWY89_001494"
     CDS             13864..15144
                     /locus_tag="PWY89_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2066538.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="PWY89_001495"
     CDS             15354..16160
                     /locus_tag="PWY89_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2066539.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="PWY89_001496"
     CDS             16172..17392
                     /locus_tag="PWY89_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066540.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="PWY89_001497"
     CDS             17382..18968
                     /locus_tag="PWY89_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2066541.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="PWY89_001498"
     CDS             19007..21145
                     /locus_tag="PWY89_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2066542.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="PWY89_001499"
     CDS             complement(21513..22496)
                     /locus_tag="PWY89_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL2066543.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="PWY89_001500"
     CDS             22874..24265
                     /locus_tag="PWY89_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2066544.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="PWY89_001501"
     CDS             24281..25321
                     /locus_tag="PWY89_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2066545.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="PWY89_001502"
     CDS             25340..26425
                     /locus_tag="PWY89_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2066546.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..27420
                     /locus_tag="PWY89_001503"
     CDS             26458..27420
                     /locus_tag="PWY89_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2066547.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            27413..28840
                     /locus_tag="PWY89_001504"
     CDS             27413..28840
                     /locus_tag="PWY89_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL2066548.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            28842..>29251
                     /locus_tag="PWY89_001505"
     CDS             28842..>29251
                     /locus_tag="PWY89_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066549.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTS"
BASE COUNT         9454 a         4737 c         5833 g         9227 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    26701 ttacaaaatg agaaatattt aaaatatgta ttattatctg gtgctgatta tccaatcaaa
    26761 gataatgagt atatttataa ttattttaaa aagaatagtt cagtggagtt tattcgtgga
    26821 atagatctag atcaaattaa acataaagaa ttttattata aacatataga tgtatatcaa
    26881 aaacatgatt atcctagaat taataggaat aacacaactg catttaaaat ttttagagct
    26941 ataattaatc gttgtctgag gatgattaaa cttcccccaa aaattagaca ccataaattt
    27001 gatttgtatc atggttctca atggtgggct ttgtcgaaag aatgcctaac agaattaata
    27061 caaatgtatg aacaaaatca acaagactac ctgaatttta aaataggtat gtttgcccca
    27121 gatgaaaaat tttttcatac actttttttt aattcctcgt tcaaaaataa aaatgttatt
    27181 ggaggaccag atttaccact tgaattgaaa aatattgaag aaactacttt acaaacttcg
    27241 aaattagcaa atattcatat tattgatcct tccatgaaca aatggtttga tgaaagggat
    27301 atagatcaaa taaaagaaag tgaaaaatta tttgtaagaa aagttaaatc ggattactct
    27361 gataaactat taaatagaat tgataaagaa attttgaaag ttggaaatat agatgaatag
    27421 aatagtacct gttttagaga agagtttatt aagtcgagat gcgtttcgag taataaattt
    27481 aatcatttgt ctgctttatt ttatgcctag tacttttaat gtaactaata tcccgatgaa
    27541 aatagctttt gcttggggtg gaattatagt tttgtatgag gtttttataa agaaaaattt
    27601 ttttaaaatg cgtttttctt ggctactgat tggagcaatg attagtttta tcttaactat
    27661 aatcattaac agagaaaatt ttttaattcc atctatttat aattttggat atttgctgat
    27721 tactttgata gtaatttttc ctgcagactt ttcacaaagt gaagaggaaa aaaagaaaaa
    27781 aatggtgtat tttaataatg ttttcattat cataattttt ttagctgcgc taatatctat
    27841 ctatcaattt atttttttga tttcttatca tgtcccaaca ggaacgccag gattacttgc
    27901 aagacaaggt tttattgaac atcggttatt tggtgtctat acaagtccaa atgttggagc
    27961 tatgtttgga tatacttcta ttattttaag tttaattagt gtattaattt caaaaataac
    28021 aaaaacgatt cgtgtttttt atatgatgaa ttttatcgta caaatatttt atattacact
    28081 ttcttcgtct agaggtgccc aggtagtaat ttgttctttt ttagtattat ttcttttgtt
    28141 gtttgccaat tcaaaattta gaaagagcat tagagaagta ctacctgtaa aaatcaaatg
    28201 gatggtaagc atattattag ttctattggt atatttttct tttggtacta attatgctaa
    28261 ggatttttta gcaacaattc ctgtatcgat tgatagaaaa gtagaagtta tagagtcaaa
    28321 tgaatcagat aaaactccac ttccagaaaa agaagtaata ttacagcatt ctagtgataa
    28381 ctccgaaatt tcagctggtc gttttacgat ttggaaagct gcgcttaaag ttataagacc
    28441 aactttgtta tttggttatg gagagaccga tttctatggg aatagcgatg caaaattttt
    28501 gaaaaaagta gatcttgata ctacagatgt gaatgaacta aaaagagcac atggaaacat
    28561 gcataatgga tttcttcaag tacttgtatc aagtggtata attgctttta tatgtatcta
    28621 tgctttctat gtgttaaata tctttagttt actcaaaaca ttttttagaa gtaaagtgaa
    28681 ttatgcgttt cttggtattg ttttaatttt tatactatct atttttatag atgaaatggt
    28741 ggaggctcat gtacttttca ataagcgcga tgtcatttca attgttttct ggtattattt
    28801 gggaagcatt tcttacttgt atttaaaaaa ggagaattaa tatgaagaca gctttcattg
    28861 cttggactcc tttacatttg attaatatat taaatgttta tcaaaatttt taccgagaat
    28921 caaaaggaga tttatttatt tattctgaat ttgaaaacgc tagaagatat agtgagcgta
    28981 taaaagaatt agggctattt aatgatgttg tttttgtgaa tcaccggaaa atcggttctt
    29041 cctttaaacg taaaattaac ttgttaacta accataatca atttttctcg gatgatttat
    29101 ccgtttatga tgaaattttt atacaggggg aaaattattt cacaaaaata cttttctcca
    29161 atgtttacaa aaaaaatcca gatgtaaaac taaattatat agaagatggt ttgggtactt
    29221 atttgaagaa gagtatattt tcaacaagta a
//
LOCUS       DANKBF010000032        29080 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000032 DANKBF010000000
VERSION     DANKBF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            68..808
                     /locus_tag="PWY89_001506"
     CDS             68..808
                     /locus_tag="PWY89_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2066550.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="PWY89_001507"
     CDS             complement(1232..1402)
                     /locus_tag="PWY89_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2066551.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="PWY89_001508"
     CDS             complement(1596..1775)
                     /locus_tag="PWY89_001508"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066552.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="PWY89_001509"
     CDS             2162..3274
                     /locus_tag="PWY89_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2066553.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="PWY89_001510"
     CDS             complement(3352..3645)
                     /locus_tag="PWY89_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL2066554.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="PWY89_001511"
     CDS             3813..4127
                     /locus_tag="PWY89_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL2066555.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="PWY89_001512"
     CDS             4148..6127
                     /locus_tag="PWY89_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2066556.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="PWY89_001513"
     CDS             6140..6466
                     /locus_tag="PWY89_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL2066557.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="PWY89_001514"
     CDS             6482..7000
                     /locus_tag="PWY89_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066558.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="PWY89_001515"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="PWY89_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2066559.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="PWY89_001516"
     CDS             complement(8436..9941)
                     /locus_tag="PWY89_001516"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2066560.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="PWY89_001517"
     CDS             complement(10033..10623)
                     /locus_tag="PWY89_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2066561.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="PWY89_001518"
     CDS             10802..12448
                     /locus_tag="PWY89_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2066562.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="PWY89_001519"
     CDS             12497..13216
                     /locus_tag="PWY89_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2066563.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="PWY89_001520"
     CDS             13695..14642
                     /locus_tag="PWY89_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2066564.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="PWY89_001521"
     CDS             14654..15574
                     /locus_tag="PWY89_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2066565.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="PWY89_001522"
     CDS             15615..17072
                     /locus_tag="PWY89_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2066566.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="PWY89_001523"
     CDS             17417..18031
                     /locus_tag="PWY89_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2066567.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="PWY89_001524"
     CDS             18024..19748
                     /locus_tag="PWY89_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2066568.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="PWY89_001525"
     CDS             19745..21196
                     /locus_tag="PWY89_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2066569.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="PWY89_001526"
     CDS             complement(21669..23822)
                     /locus_tag="PWY89_001526"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL2066570.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="PWY89_001527"
     CDS             complement(23824..24870)
                     /locus_tag="PWY89_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2066571.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="PWY89_001528"
     CDS             25020..26309
                     /locus_tag="PWY89_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL2066572.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="PWY89_001529"
     CDS             26322..29024
                     /locus_tag="PWY89_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2066573.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9455 a         5025 c         5898 g         8702 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tagaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctttgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgattcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggcttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atacttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggac ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aattttagaa
//
LOCUS       DANKBF010000033        28603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000033 DANKBF010000000
VERSION     DANKBF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            237..1877
                     /locus_tag="PWY89_001530"
     CDS             237..1877
                     /locus_tag="PWY89_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL2066574.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="PWY89_001531"
     CDS             2031..3353
                     /locus_tag="PWY89_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL2066575.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="PWY89_001532"
     CDS             complement(3415..3846)
                     /locus_tag="PWY89_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2066576.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="PWY89_001533"
     CDS             complement(3910..4746)
                     /locus_tag="PWY89_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2066577.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="PWY89_001534"
     CDS             complement(4936..5238)
                     /locus_tag="PWY89_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL2066578.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="PWY89_001535"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="PWY89_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2066579.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="PWY89_001536"
     CDS             complement(5692..5958)
                     /locus_tag="PWY89_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL2066580.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="PWY89_001537"
     CDS             complement(6212..6937)
                     /locus_tag="PWY89_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2066581.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="PWY89_001538"
     tRNA            complement(7303..7373)
                     /locus_tag="PWY89_001538"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="PWY89_001539"
     CDS             7490..8242
                     /locus_tag="PWY89_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2066582.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="PWY89_001540"
     CDS             8258..8737
                     /locus_tag="PWY89_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2066583.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="PWY89_001541"
     CDS             complement(8988..10568)
                     /locus_tag="PWY89_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL2066584.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="PWY89_001542"
     CDS             complement(10561..11304)
                     /locus_tag="PWY89_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066585.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="PWY89_001543"
     CDS             complement(11480..12256)
                     /locus_tag="PWY89_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2066586.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="PWY89_001544"
     CDS             complement(12504..13340)
                     /locus_tag="PWY89_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL2066587.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="PWY89_001545"
     CDS             complement(13351..14280)
                     /locus_tag="PWY89_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2066588.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="PWY89_001546"
     CDS             14472..14825
                     /locus_tag="PWY89_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2066589.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="PWY89_001547"
     CDS             complement(14899..15555)
                     /locus_tag="PWY89_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2066590.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="PWY89_001548"
     CDS             15781..17715
                     /locus_tag="PWY89_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2066591.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="PWY89_001549"
     CDS             complement(17801..19024)
                     /locus_tag="PWY89_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2066592.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="PWY89_001550"
     CDS             complement(19018..20916)
                     /locus_tag="PWY89_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL2066593.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="PWY89_001551"
     CDS             complement(20910..22028)
                     /locus_tag="PWY89_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2066594.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="PWY89_001552"
     CDS             complement(22111..22944)
                     /locus_tag="PWY89_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2066595.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="PWY89_001553"
     CDS             complement(22937..23854)
                     /locus_tag="PWY89_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2066596.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="PWY89_001554"
     CDS             complement(24081..25205)
                     /locus_tag="PWY89_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2066597.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="PWY89_001555"
     CDS             25388..26296
                     /locus_tag="PWY89_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2066598.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="PWY89_001556"
     CDS             26437..28050
                     /locus_tag="PWY89_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2066599.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DANKBF010000034        27852 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000034 DANKBF010000000
VERSION     DANKBF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            161..478
                     /locus_tag="PWY89_001557"
     CDS             161..478
                     /locus_tag="PWY89_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDL2066600.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            475..636
                     /locus_tag="PWY89_001558"
     CDS             475..636
                     /locus_tag="PWY89_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2066601.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            639..938
                     /locus_tag="PWY89_001559"
     CDS             639..938
                     /locus_tag="PWY89_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066602.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            938..1252
                     /locus_tag="PWY89_001560"
     CDS             938..1252
                     /locus_tag="PWY89_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2066603.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            1245..1775
                     /locus_tag="PWY89_001561"
     CDS             1245..1775
                     /locus_tag="PWY89_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2066604.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1772..2041
                     /locus_tag="PWY89_001562"
     CDS             1772..2041
                     /locus_tag="PWY89_001562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066605.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            1971..2174
                     /locus_tag="PWY89_001563"
     CDS             1971..2174
                     /locus_tag="PWY89_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2066606.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            2171..2458
                     /locus_tag="PWY89_001564"
     CDS             2171..2458
                     /locus_tag="PWY89_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066607.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            2945..3358
                     /locus_tag="PWY89_001565"
     CDS             2945..3358
                     /locus_tag="PWY89_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2066608.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            3978..4142
                     /locus_tag="PWY89_001566"
     CDS             3978..4142
                     /locus_tag="PWY89_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066609.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            4554..5099
                     /locus_tag="PWY89_001567"
     CDS             4554..5099
                     /locus_tag="PWY89_001567"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2066610.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            5100..5417
                     /locus_tag="PWY89_001568"
     CDS             5100..5417
                     /locus_tag="PWY89_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2066611.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(5574..5738)
                     /locus_tag="PWY89_001569"
     CDS             complement(5574..5738)
                     /locus_tag="PWY89_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HDL2066612.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            5840..6361
                     /locus_tag="PWY89_001570"
     CDS             5840..6361
                     /locus_tag="PWY89_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2066613.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            6348..6821
                     /locus_tag="PWY89_001571"
     CDS             6348..6821
                     /locus_tag="PWY89_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2066614.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(6828..6986)
                     /locus_tag="PWY89_001572"
     CDS             complement(6828..6986)
                     /locus_tag="PWY89_001572"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HDL2066615.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            7079..7840
                     /locus_tag="PWY89_001573"
     CDS             7079..7840
                     /locus_tag="PWY89_001573"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2066616.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            8060..9379
                     /locus_tag="PWY89_001574"
     CDS             8060..9379
                     /locus_tag="PWY89_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2066617.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            9395..10615
                     /locus_tag="PWY89_001575"
     CDS             9395..10615
                     /locus_tag="PWY89_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2066618.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            10650..11327
                     /locus_tag="PWY89_001576"
     CDS             10650..11327
                     /locus_tag="PWY89_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2066619.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            11324..12517
                     /locus_tag="PWY89_001577"
     CDS             11324..12517
                     /locus_tag="PWY89_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2066620.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            12532..12858
                     /locus_tag="PWY89_001578"
     CDS             12532..12858
                     /locus_tag="PWY89_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2066621.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            12862..13197
                     /locus_tag="PWY89_001579"
     CDS             12862..13197
                     /locus_tag="PWY89_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2066622.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            13194..13589
                     /locus_tag="PWY89_001580"
     CDS             13194..13589
                     /locus_tag="PWY89_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066623.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            13586..13957
                     /locus_tag="PWY89_001581"
     CDS             13586..13957
                     /locus_tag="PWY89_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066624.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            13973..14533
                     /locus_tag="PWY89_001582"
     CDS             13973..14533
                     /locus_tag="PWY89_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2066625.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            14658..14969
                     /locus_tag="PWY89_001583"
     CDS             14658..14969
                     /locus_tag="PWY89_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066626.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            14993..15184
                     /locus_tag="PWY89_001584"
     CDS             14993..15184
                     /locus_tag="PWY89_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066627.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            15184..19032
                     /locus_tag="PWY89_001585"
     CDS             15184..19032
                     /locus_tag="PWY89_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL2066628.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            19033..19905
                     /locus_tag="PWY89_001586"
     CDS             19033..19905
                     /locus_tag="PWY89_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL2066629.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            19902..20951
                     /locus_tag="PWY89_001587"
     CDS             19902..20951
                     /locus_tag="PWY89_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066630.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            20955..21779
                     /locus_tag="PWY89_001588"
     CDS             20955..21779
                     /locus_tag="PWY89_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066631.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21797..23857
                     /locus_tag="PWY89_001589"
     CDS             21797..23857
                     /locus_tag="PWY89_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL2066632.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            23874..24023
                     /locus_tag="PWY89_001590"
     CDS             23874..24023
                     /locus_tag="PWY89_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066633.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            24020..24466
                     /locus_tag="PWY89_001591"
     CDS             24020..24466
                     /locus_tag="PWY89_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066634.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            24468..24605
                     /locus_tag="PWY89_001592"
     CDS             24468..24605
                     /locus_tag="PWY89_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2066635.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            24640..24882
                     /locus_tag="PWY89_001593"
     CDS             24640..24882
                     /locus_tag="PWY89_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDL2066636.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            24898..25095
                     /locus_tag="PWY89_001594"
     CDS             24898..25095
                     /locus_tag="PWY89_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2066637.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            25106..26122
                     /locus_tag="PWY89_001595"
     CDS             25106..26122
                     /locus_tag="PWY89_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2066638.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            26237..27445
                     /locus_tag="PWY89_001596"
     CDS             26237..27445
                     /locus_tag="PWY89_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066639.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            27429..27599
                     /locus_tag="PWY89_001597"
     CDS             27429..27599
                     /locus_tag="PWY89_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066640.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT         9798 a         4596 c         6092 g         7366 t
ORIGIN      
        1 ctttctggag ggttttaaaa aaataataaa gtattaaagt aaaaaataaa aagtaaatta
       61 tataaaaaag aataggtttt tgggtctgac cctccaaact ctccaccaaa atcttcctaa
      121 atgctttgat accaacgatt aaacagtctg gagggttaca ttgaaatcag aacatcaaat
      181 acaagacgag atcagaattg ccttatcaaa acatggttgc acgatttttc gatccaatgc
      241 gggacaagtg acaacgatcg aagggcggca attttacggg atgccaaaag ggttcccaga
      301 tctatgtgga catcgtaatt ctgatgggaa agcaatttat atagaagtaa aaaatgaacg
      361 cggtaagttg cgtgaagatc aaaaaaggtt tgctgaattt ttaaacggtc aacctgtact
      421 atacggtgtt gcacgatcag tggaagaagc attgaaaatt gtggaggaat catcatgaca
      481 acagaagaag tgattcaaat gcgtattcga agccttcagc gtgagattga cgatctggaa
      541 cggacaaagg cagtgatggt caatgaaacg gcgaggaagg caatcgattt gcacatagag
      601 aatttaagaa gggaaatcca tcgattggag gaatgagcgt ggataagaaa gcagcaatga
      661 aacgaatcat cgaactgaca cattctgaga attggcaaga agacaaagaa atagttgcag
      721 aagtccaaaa gctcggtaaa tcaatgtgga gtgaaaaaac caaacggaga acaccgagaa
      781 aaattgcaat ctggcatggt gaccgaattc tagtaacagg tactgctgaa cagttatctg
      841 aaattactgg attaagcaaa aacattatct gggatagagc taaacgcgag aacgttgatt
      901 cgaaaggtcg tcaatttaaa cattgggaga agaaataatg gaggaactaa tcacaaaagt
      961 agagcagtgg gcaaaagata agggattgga tcaagctgat tccagcaagc aaatgttaaa
     1021 aacgatcgaa gagattgggg aagttgccgc ttctctagct agaaaagatg aatatggttt
     1081 aagagacgga attggagacg tagtagtaac cttgattatt ttagctatgc aaaatgatat
     1141 ggatttgtac gagtgtctga accaagcata caacgaaatc aaaggacgca caggaaaaat
     1201 ggtagatggt gtattcgtga agtcgagtga tttggaggac agcgatgaat aaaaaagtat
     1261 taattgataa acaagcattg attgatgaat taatgaaaat acctggtgtg ggatctaata
     1321 gtgacgcttt agaaacgatt aaacgtttcc catcgtatga accgcagaag ccagttgtgc
     1381 ctaagtttgt ggcggaacta ctcgactatt atcgtcagtc aacagatgtt gatttattag
     1441 ctttattgat aactttccat gattggtatt atcgcaaaac taaagacggt gagcatgaag
     1501 aagcaattga ttggctagtt gatcatcctg aaatttatat gcgtgcatgg ctgtttggct
     1561 acgagctcga gaaagagccg ttgtattgcg taataatcaa tggtagatat cttgtcaaag
     1621 tattcagcaa tacaaacgtt gtcatattag ttccagcaga tgaattttca cagtacgtta
     1681 ctcaaaaatt tcaattaact gaaaaacaaa tcaaagcaat tgatgaaaga tactggccgt
     1741 ttgctgtgcc agtggatgag gtggtagaag gatgagcgac ccatacatta ctaaattaga
     1801 aaagttgtcc gaagattttg agaagaataa aaataaacta actaaagata aacttataga
     1861 actggttagg ttggttatca gaggagctat tacaagagaa aaggcgcagc aaaacataga
     1921 aatttttgct cgtagtgtcc gagatggatt agagaagaaa gttaaacaaa atgaacaaca
     1981 gacaccgcag aataacaaaa ctaagaaaac gggaactgaa tgtactaaag acaaagtttg
     2041 aaaaagaata tggaatttca gcagaagaaa catataaagt ggcaagtcag tgtgttgctg
     2101 atgcgagcgg cgctattcgt aagtttggaa tttcgatatt aagtgatgat cgtaaatggg
     2161 aggaaaaaga atgaaactaa aagacggatt ttactccagc agtcatggta tcggcggttt
     2221 aatgctagat atgccgacaa agaaccctaa aacacgtgag aaaccaaaat tcaaagtcgg
     2281 tgacatggtt cgctgtgaag cagaaggatt catctatcca ttttgtggat atgtagaaca
     2341 tctctataat cactcagcaa tcattcgtat tgaaaacacg atggaatgtg acaagtggtt
     2401 agcgaaaagc aaagagaatt tagcagtagc gagattggtg gatatggagg ttatataaac
     2461 agcgaaaaaa tgtacttcta agagaaaaat ttatttttta taaaaatagg aaagtatcat
     2521 tatgaaaaag accacagaga taaataaacc taccacataa aaaatgaata tttcttgcga
     2581 ttcaatatag gtatatttaa caatttgtac aataaaaaat actataggta aaaaagataa
     2641 catgaggagc ttatttgttc tagagtgttt gacaaagtaa caaaatgcga cgataccaat
     2701 acaaacggga atccaaaata acagcatgct ccacatagtc actcaaccat ctcctatacg
     2761 ctttattatc aatagataat atcatttttc gtaataaaga taaaaaaata agtaaggctt
     2821 ctcattaaaa tataaaaaag acagccgacc actgactgcc tatataagag tattgaaata
     2881 aaaagctgct gatataataa attccacaag tttattatat cacataaagg agcggtttga
     2941 cttgatgcaa ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt
     3001 gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc
     3061 gccgattatt acggatatgc cgaaggcgcc gaagcacggc aataaggcag aagacgcaat
     3121 cattcagatg atggatatag aagcggagag agacgcgatt ctagcagcct tgatggctct
     3181 tagtctgatt agtcgtcaga tactctacta tagcttttgt gtgccagata gcttctcaaa
     3241 ctacagaatt agccgtgaag tgggttattc agaaagaagt atacaacgga tgaagtcgga
     3301 agctctaata gagtttgcag aagcatataa acacggaaga ataattgctt ataaataatt
     3361 tggcggcttt ttggcggaat gatggcggtt tttagctatt taccagtgat attatggtag
     3421 tgtcgaaaga ttagtgatag gtctaagaca aaataataat aaaaggaaca tcgttttatt
     3481 attgtttcac aattaagctt cgatagacag caacggaaat attaagaata aggatgtgaa
     3541 ttttaactcc ttctaaattg ttcttattat ctatcatccg ttgctgtcta ttgttttttt
     3601 aattattcac acgataacta aaggtgagcg aagagaaatg attccattaa taatttcaat
     3661 ttttgcgctt tgtcttaatg tctatatgat tggatttaaa aatgggcaaa ataaaagata
     3721 gtagtaacta agaaagaaat ttttatattg tcactgtggc ggaaagggta gacgctaagc
     3781 atgtgtgcta ggtcaatgct tcggcaacta tgcaatgttc gattcattgc cagtgacttt
     3841 ggcaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta cgcgttttgt
     3901 gcatcgtgta ggttgctatt acatattaga tcactcgttg agtggtcttt ttattttaca
     3961 taaaggaggt aacaacgatg tatagaccac aatacttaga acagaagtat gaagtaatca
     4021 ctgtgcaaaa cggtaacggt gagatagtac gaaagtatag aagaccaata aagagcgata
     4081 catataaacg aaaggaaaac aatgaagtta tttcttttcg cagaaggaga aaagccaaat
     4141 gagaaactac tggtatgtat cgctaactaa tgaatatcct cgaaccattg atgattgttc
     4201 agtgcgtgtt gtgcgttctg tacaaatcaa agggaagtac tctattgtcg aaatgctaag
     4261 agaagctaca ccaaacgaag tggataaatg caagctgata tattgcggtc atggctattg
     4321 gaaagacgag tatatccaac aaaacattga gaggtggata gatagatgag ttacctcgaa
     4381 catttgaaac gttgctacat gcattctaag aataaacttc ctgacagcta cactgtagat
     4441 gatgttgcta tacatgtatt gaagactgag agccatagca gtcctgatgg tagcagtaag
     4501 gagcagacgc tcgcgtggtt caagttcttt aagtggatta aggaggaaga gtaatgcaca
     4561 tgcacatagg agaaaagata tataaattga gacggcttaa taacgaaaca atgaacgact
     4621 tagttaaaca aactggagtg tcaaaatcat ggttatctga tattgagtct ggaaaaaagc
     4681 aacgcgttga ttttaataag atatgtgcaa tagccaaaca ttataaagtt agcttagaat
     4741 actttcggga tgattatttg gatagtgatg aacacgagga tattattcct ttgacaggcg
     4801 aaacaagagt taaatcagtg aaatcgcttg aacaaagcct aaagtatctg aataacaaac
     4861 aagaacctaa ccttctagag atcaaactaa aagatactga ctcagtacca gaggtttggt
     4921 acaagggtga gaggttggat gaattgccta aaggattagt agatgtctcg tatcattgga
     4981 agactgatga ttttactaat gatgatagag gagcgaacga catcacgatt caatactttt
     5041 ctagctttaa tgataagtat ccagataaga aaacaatcgg acacaagaga gatatgtaaa
     5101 tgaaagaagt tagacctaga gacgaaatag acaaactata caagaccaaa cgatggcgag
     5161 acctaaggca agtagtaata gctagggact tcggcatgtg ccaagagtgc aagcgtcgag
     5221 ggcggaacac aaggggcacg atcatccatc acatagtcga ggcgagggaa gacctgtcac
     5281 tgttctggtc cgtagataac cttgaatgta tctgtgtagc ttgtcacaac agagagcatc
     5341 cagagagatc aggcgggaag aagaaaccaa aacctaaatc acatatcgtt aaaatgtatt
     5401 caactcctga aagataagtt tgcagcgaaa tgaaggtagc ccccctactc taaaagatta
     5461 aagagtaagg cttgagaaga acggtgctgt ccttccttcg taaaaagacc gcttttcaag
     5521 ttttttggag aaaaaggaaa aagccgacca atttaagccg gctttggacg aagctatttc
     5581 ttagtccatt tgtttccttt ttgagaagta gggggtaatc ggtcgcctgg atcaattttt
     5641 acttcttgtc cgccttggac atttccacca cgaggaccca cttctttgta ggttcctttt
     5701 ggtttgttgt cttcgccggg tttatagagt tctcccatag gaatccctcc ttaacaaatt
     5761 tcggcacagc actgccgata acctaattat aaggattgtg ataacgattt caatctatct
     5821 tttgaaagaa ggtgatatta tgccgcaacc agcgaagagt gcaaaattac aattattaaa
     5881 cggaaaccca aataagaaga ataccgaaga actccgcaag cgagcggccg cagaagacaa
     5941 attaaaaatg gctactgaca aaatcaaacc gccgttatgg ctagattcgc taggaaagga
     6001 tacctttgag tttatcgccg atgaattgct gtctgtggat ttaatcagta atccggacgt
     6061 ccatacaatg gctctatact ccaattggta ttcgcaatac gtttctttag aaaaacagct
     6121 tcgaaaacta caacgagagt acaagttgaa ctatgcgctt gcgaaaaagg aggcagaggc
     6181 gagaggtgag ccgtttaatg aacctaatga attaattggt aatccgctct ctcggcagat
     6241 ggatacagcg tctcggaatc tccgttcttt tggcgctgat ttaggactat caccagcagc
     6301 cagagctaag ttagctatta agatggctga tgatggtggt gatgacgatg acgacttcta
     6361 atattttgga tatgtcttac acagaacgtg tggactattg gcaaagctat cttgaggagc
     6421 aagcttcttg gggtggcttt ttaaaatgtc catatccgga attgttaact acttggtatg
     6481 cggaacgatt aatcgatgga agcataccag ccagcaaaga aaatattcaa gctgctaaac
     6541 ggcatatgcg tgatttgcag cgccaaggaa cagatgattt tccttggatc tttgacgaag
     6601 aaaaaggtca ccggcctatt agatatatcg aaaaaaaatg taaaccaact gaaggcgact
     6661 ttggttcgtt tgttttgcaa ccttggcagc atttcataat tggatccatg tacggatggg
     6721 tacatcgtga tacaggagag cgtcgcttcc gcgaggctct tatttttgtt ggacgtaaaa
     6781 acggtaagtt ttgccgttta aaaatcgggc aaaatcggtg aaaaccttta tttttggctt
     6841 tcttttagta ataattcgat agctttatca agcaatttag acataggaac catagtttct
     6901 tccgacattt tttttaatcc ttcgtataaa tctttatcaa ttgcgttaga aattctaatt
     6961 ctgtttttta gtccgtattt gtccatgtgt gacactcctt tttattttac tataacatcc
     7021 gattccaccg cttgcaacta ccatcaattg atggtagtat ataaaagagg tgagtagatt
     7081 gaaaaaatac attatttaca aacacttaaa taaaataaat ggcaaaattt atatcggtgt
     7141 cacaaatgat attggcagaa ggtggaggag cggtgggatt gaatataaac caccaaagaa
     7201 cgaaacccaa caccacagaa gtttttggaa tgcaatacaa aaatacgggt gggaaaattt
     7261 tgatcatttg attattgaag aagacttaac gatgaaagaa gcttttgaga aagaaaagtt
     7321 ttatattgaa ctttatgact caactaataa gaaaaaaggt tataacatcg caaaaggcgg
     7381 aaatggcgga ataatatata aagttcaccc caaaggaatg ctagggaaaa aacaatctaa
     7441 agaattttct tcaaatcaca gtaaatgggc aaagaaccat aagaataatt gtatgacaaa
     7501 cggtgatgtt gtatggggtg ttactcacga acacccaaaa ggcatgttgg gaaagcacca
     7561 cagcaaagaa tcaattatga aaaagaaagc atatagtggt gaaaatgctg taacctcaaa
     7621 accaattgtc gcaattgaac gggatggaac taaaagagaa tttcacagcg ctaagctttg
     7681 catggcttac tattcgatta gtacatgtgt tttttaccga cttttaaaag atggcgcccc
     7741 ttatgtgata aatcctaaag cgaactacag aaataaagaa aaaatattag ctatcgaagg
     7801 aattatgttc aaacataaag aagataccga ggtaagttag tagattgcga aaggttacta
     7861 actaccgtaa cgcgtaggag ttgaataaat ataatgctcc caagagtgtc cgacagcaga
     7921 taatttgctg aaaatgtacg ctaaactggg ccagaactga ctgaccgatg aaaatgaggg
     7981 tgacctccag agtgcgagat aaaaagctcg cagataataa caatcgaaaa catcactaat
     8041 ttcaggcctt tccacataca tggtcgctta tgatgatgaa caaggcgcca acgtttacgt
     8101 attggcaaat gctcgtgatc aagcaagctt gttgtttgat aaggccgcag aaatggtcaa
     8161 acaatcgccg gcgctcttta agaaatttgg taagcctaaa cgatcaagta ttaattatgc
     8221 tcccgccttt tctaaaatgg aaccacgcgc ctcagatagc cggaaattgg atgggctaaa
     8281 cactcacttt ggtatttttg acgagatcca cgagtttacg aattacaatc tgatcaacgt
     8341 tatcaagaaa tcaagaggaa ccagaaaaca gcctctgata gtttatatca caactgctgg
     8401 atatgtatta gatggtccgt tgatgtctta ttttgagcaa ggtgtggact gtttggaaca
     8461 tttggaagat gacatcgatg aacggacttt ctattatctg gcaaaacttg acagtgcgga
     8521 agaggctgat gacccaagat tatggatcaa agccaatccg aatatttgtc taatgaattt
     8581 tgttggcatg ctagatgact atgttaagga taaaaaagat ccaaaagaat atgctgactg
     8641 gattaccaag caatttaact tgttttccga tatcgatgag ctgtcatttg tcgatatgcc
     8701 taccattaaa cgaaacaata aaaccatcga tattgaaacg ctcaaaggta agaagtgtgt
     8761 cggtggtttt gacttgtccg aaacggaaga ctttaccgca gccgttttag aatttccgct
     8821 tgaaacaggc gaggtattca ttttgcaaca cacatggatc ccacaagcta gatttgatcg
     8881 agataacaat caagagcgta tcaaagcgtg ggagaaggtg ggagatctaa cgattattcc
     8941 tggtgattac gtcaattatg aatacgtctt aaattggttt gtagaaaatt cgaaaatcta
     9001 tgacattgta aaaatcaatt atgacaaggc caaggcgcta cgattaaata aagaactaga
     9061 aaatgcagga tttgaaacca acgagattcg gcaagggttt ctatcattag gtgggccaat
     9121 gcaaaacttc aaggaaatgc tattggacgg taaggtgatt ttcaacaatt ccaagcttta
     9181 ccgatggtat ctatccaacg tcaagctggt gatggatcgc aactcaaact ggatgccgtc
     9241 taagcagtcc aagagtagaa aaatagatgg ttttgcagca agtttgaaca gccacgccga
     9301 agtgttgaat atgttggtta atcctgtcgg aaccgggaaa gtaacctatt actcgatttc
     9361 cgatttaatg aatatgtaag aaaggtgtgg aggaatgagt attttagatc gtttgcgttc
     9421 ttttggccga gcgaagccga aagcgagcaa acaagagtat tttttgaatg acccgggatt
     9481 gataccgtat ttagtcggaa aagatgaaat atcagaaggg attttttccg taattagccg
     9541 tgtatcgaac gtttttgcgt ctctccctct caaaatgata gatgtggagt ttggccaacc
     9601 ggacgactgt cctgcataca acttgttgag cgaaggccct cgatatttta caaagtttga
     9661 ttttttccgg gacgtggaag ttttgagaaa ctaccaaggg aatgcgtatg tgcagatttt
     9721 ccgaaatatc aatggagaag tagcagatat ggcgttagta aaacctggtg cttgccatcc
     9781 agtgattgat atggatagcg gggagcttta ctaccaagta actgcgactg acaaaggcag
     9841 ttacaagcaa gttatctatg tacattacat ggaaatgctt cactttaaac aaccgaggtt
     9901 tggcggcttg gaaggtacag accccacaaa agtattaacg aataccctcg gatatgatcg
     9961 agaagtccga aaaatctctt taagtcagct taaaggaagt aatgaagggc taaaagttaa
    10021 gtttgctagc aatatggatg aagaagctaa aaaagctaca gttaaaaaca ttgctgattt
    10081 ttatcgacaa aacggtggac tacttgtgga agaaaacggt gtagaaatcg aacgtttaca
    10141 acgagagctg gtagacagca agcttttaga tactgataaa gtatctcgct ccagaatcgc
    10201 tatggtctac aatgtgccgg agcatttcat cgggaataac cagtcgagtt actcctctca
    10261 ggaacagctc aatatggaat ttttgacgta caatctagta ccgaccgtta atcaatatga
    10321 agcggaacta aataagaaaa cactatcgag aaccgaaaaa gcaaaaggtt atcgatataa
    10381 gtttaatgtc gcaagtttgc taagagctga cactcaggca aggggacaat tctatcaaat
    10441 catgcgacga ggcggagcat actcagcgaa tgatgtccga agatttgagg acttgcaacc
    10501 gataaataaa accggtatgg atgattacca tatttccgga gacctatatc caatcgatat
    10561 ggatccaaca ttaagaaaaa caacctcgtc taaaagcgta gccgaaaacg gttaggtttt
    10621 tttagtttgc accgaaggga ggtggaaaaa tgaaaaaagt gacgttaagc ggcgatgtcg
    10681 tggataacga taccgcgtgg ctttatgact ggtttgggat caattgtatc tcaccaggga
    10741 aaatttctgc cgctcttaca gaagcagcgg gggatgaagt agaacttgat atctcatcga
    10801 acggtgggga tgtcctagcg gcaagcgaaa tatataccgc tatccgtgcc tatccgggaa
    10861 aggtatccgg aaatgttgtg agtattgcag caagcgctgc aagtgtaatc gcttgcgctt
    10921 gcgaaccgct tagaatctca cctacggcac acatcatgat tcataacgca tgggtgacca
    10981 ctagtggcaa cgctgaggaa ttaaaagcca atgcagaaat gttaagcagt gtggatgagt
    11041 ctattgttaa tgcttacgag atcaaaacag gactcgatcg gaaaaaactt gctgatttaa
    11101 tggcgaaaga tacttggtta aatgctcaaa cagcagtagc agaagggttt gcagatgaaa
    11161 ttatgtttgc agaagcacca gtaacggtac tcaatgcctc tcaaccggtt attccaaaaa
    11221 acgcagtaac taagttgaaa aatttaatac tcaaagcgga agcaccgcaa gaagaaacac
    11281 tcttacagaa aaaactaaaa gccttaaatg gagggaaaaa cgaatgaatt tagaacaatt
    11341 aaaaaatgcg tgggtcgagg cgggaagtaa agtctctgac ttaaatgcac aactcaatgc
    11401 agcattggtt gacgatgaaa aaacagaaga agatgtagta agtttgcaag cacaagtaaa
    11461 agcagcacgg gctaaacggg acggattgaa agagcaagtg gcaaatatgg aagccgaaca
    11521 agtcttaaac gtcaaaaaag aaccattaga taaaaaagat gaaaacttga aaaacaagtt
    11581 tatcaaagac tttaaagcga tggtcaatgg tgatcctgct attatggcta ctttgacatc
    11641 taatacggat gaatctggta atgctatcgg attgactatt cctgtagatg tgcaaacgac
    11701 tattcatact ttggttcgtc ggtttgactc tttgcaagaa tacgtaaacg ttgaaaaagt
    11761 gaccactacc agcggttctc gcgtttatga aaaatggtct gatattacac cgttgactgc
    11821 tttggatact gaagacggtg aaatcccagc aaatgatgat cctgcacttt acttgatcaa
    11881 atacttgatc aaacgctatg caggtatttc cacagtaact aacagcttgc taaaagatac
    11941 tgccgaaaac attttggcat ggttgtctaa atggatcgcg aaaaaagtag ttgttactcg
    12001 caatacaaaa atcttggcag ctattgatgg aatcaaagcg gcacaaaaga aagatgttac
    12061 agatgttgat ggaattaaag atatcgtaaa cgtccaactt gatccagcta tcgaagctac
    12121 atctatgttt attacaaacc aagatggctt caatgtttta gataaagtga aacgtgctga
    12181 tggatcttac ttgttacaaa aagacgtagc ttctgcaact ggatatacct tcttaggtaa
    12241 accaatcaag aaaattgctt ctcgtttctt gccaaacaaa ggtacgcaag ctactcctaa
    12301 atatccactg tacattggtg atctgaaaga agccgttaca ttgtatgatc gcgaaaacat
    12361 gagcttgctg acaacgaata ttggtggtgg agcttttgaa acagacacca ctaaagtacg
    12421 cgtcatcgat cgcttcgatg tgcaactagt tgatgatgaa gcggttgttt tggctacttt
    12481 tacaactatt gcgaacgaga caccggcgaa agtttaagga gctgattcct tatgattctt
    12541 gatcctaaaa tggatttagg cgaaatcaaa aacgcactaa agattgatac cgatgatgac
    12601 gatgtggaag taagccgtgc ggcacaagct gcaattgcat acattagagg ggctatcggg
    12661 aatgataagc cctcttttta taagcaagaa aatgaaacgg ttgatctgat taatttagct
    12721 atcctgcaat tagcagatca ttattacaac gctcgatcag caaccgtgag tgggaacttg
    12781 cgagagtacg atttaggttt tacaagccta atcttgcaac tcaaagcaag ttatttgctt
    12841 tttgtggagg aggagtagcg tatgcccctt atccaaacag gaaatttaaa tcaacgcatc
    12901 aagtttgtcc gagatacgac ggttaaggat gaggacgggc aagttgtccc gacttctaca
    12961 accattctta cttgctgggc aagtgtgcag acacaacgcc tgaacgatat taagacgtcg
    13021 attggtacgg ctttggaagg aacactgacg ttcattatcc gctaccaaca aaaatcagag
    13081 ctgaccaatg atatgaaagt gcgttggaat ggaaaaacgt ttgaaatcat tacgattacg
    13141 aaaggcgagt ttgcgaagga cttcacgaca atcattgcaa aagaggtttc aaaatgagtg
    13201 tagaagtcga tgcaaccgaa gtgtacaaag cgcttaggga agtaaaagca aacgttcaac
    13261 gagtggaaag cccagcactt agaaaagctg gggagtacgc tcaagaagag ttacgacaaa
    13321 acacacctta ctgggatgga acgaagtcaa acggtaaacg tggttcgtat atgcaagaac
    13381 atgctaagaa ccatgtggtt acaagctcga taaaaaacgg attggtagaa gtcggctatg
    13441 acaaagatgt ttcttggcgg atgcacttta tcgagttcgg aacaatcaaa caacgtccaa
    13501 aaggtttcgt acaaaaaaca caaaagcaaa tcgaaaaaca agtaacacaa atcattgctg
    13561 acgaagtaaa aaggaggcta ggactttgaa aacggcagta tcacaagtct attcaattct
    13621 gaatagcaat gaaaaaacaa agaacattga tttttacacc aatagtgttc cggaatcagc
    13681 tcaaacagta cctagccttc cagttggcag aattacagag atatccggca actatgaaga
    13741 ttttgcaagc aacaatcctt tgaccattca atttaacgta caggtagatg tatgggtgtc
    13801 aaccatgaaa gaggttgatg ccttttattt tgcccttgat gaggttatga gggggaatgg
    13861 ttggcaatgc gcatacacgg aacaaacaga tgacgaggac ttggaaggtg caaagcggat
    13921 tatcaaacga tatgtagcaa atatttcact aaattaaaag gagagaaaat agatggcaac
    13981 agtaggattc gagagcgtca tttttggcgt aaaaacaggt gcaggcggca ctctaaaaga
    14041 attagtagca gataagtcga aaggcggagc gatcgaagct aaaattactg gattaggcgc
    14101 aacttctaac acaacatacg cttcaaacgt accgttcttc attgcaagta aaggggtttc
    14161 gtcgccaaaa gttacgcttg acgtggcaga cttaatggat aacggcattt acagcgaaat
    14221 cattggtgct aaaaccgtgg atggtgcaaa tgtaattggt tcagaaactg aagcgcctta
    14281 cgtgtcggta gtcatggtta cagcgaacaa agaaggaaaa cgcttgttca tgggattgac
    14341 aaaaggaaaa ttcagtcatc cagatatcga catgaaaaca gctgaagaca aaggggtaga
    14401 attgcaaacc gattccatcg aaggggaatt catttctgat gaacgtggct atgtatactt
    14461 aacagccgta gaatcagaag gaatgacctt acaaaaattc aaggacttgg taaataacaa
    14521 agagggggag tagttaaccc tgcatctaca ccaacgacag atacagggac accaaaagaa
    14581 ccagaaccaa aaattgatac acaaggttag ccatttttgg ctagccttat tttttgtaaa
    14641 aacaaggagg aaaacaaatg attgaattgc aattgaaact tgacggaaag aaaaaaacat
    14701 tcaaacaaca agatatttcc gcacgtgcaa tgcgtgagtg tatcaaattt tacgagaaag
    14761 cggaaaaagc agacctaact gatttagaag cgattgattc aatgattgca attacagcag
    14821 atattttcca agatccagca gttacatttg atgctatttt agacggtttg actgcgagcg
    14881 agttagtacc ggcattagaa agtgtttttg aacaaataaa tgaactggga aacaatgaaa
    14941 aaaagcagac ggcgagcaaa aagaaataag tttttctgaa gctaggaaag caatggatca
    15001 aatctacaaa gatttaatcg aagcaggttg gacgatgaga gatgtggacg aagccgacta
    15061 tcattatttg ttacaccttt ttggagaagt ggagagtggc gaagaatatg tagatggtgc
    15121 tgatttcatc aaacaatttt tatcggctga agacttagta aaacttgagg aaggaggtaa
    15181 ataatggcag gaaaaggaca accggcagga aatatcaagc tagggattag tttagatagc
    15241 actagttttg gtaacacgct ggacgaaatc aatgcgaaag tcaaacaagc tgagtcgaat
    15301 atgcgtgcca atctaaaggc ttatgattca gcaggacgtt catacgaagc acttagtcaa
    15361 aagacgaaag acttgtctac ggttatggaa gggcaaaacg ccaaagtaag agaattaaca
    15421 aagcgccgtg atgaagcgat tagcaagtat ggcgaggaat cgaaacaagt tgctaacctt
    15481 aacacacaga taaacaatgc taccgcaaaa tataatgctt acagtcgcca gttgaacgac
    15541 acaaaaaaag aattggtgta ttccaaaaca gccgtcaatg atttatctaa tgaaatcaaa
    15601 gaaaatgaac gacaaatgaa cgccgaagta aaagcgttga aagccgctgg tgatgaatct
    15661 ggtgcttttg aagcaaaaca aaaagggcta gccaaacaaa cggaattatc cgagaaagct
    15721 atcgaagaac agcgcaaagt tgtgaaactg atggctgatg agtttggcga ttcagcaaat
    15781 gaaaccgaag atgcaaaaag ggcattagaa aagttagaac gacaaagcca aatatctagc
    15841 aggcaattag aagcactcaa aagctccagc gatcaatcag gaaaagaaat agaagatttt
    15901 ggcgacaagt ccacaaggtc agctaggaaa ctggacggac taaaagacaa attaagctcg
    15961 ctaaaaagcg cattttcgtt tggtgcagtt gctggattag cgcataacgc tattagcagt
    16021 gtagtaagtg gcgtgcaagg tttggttggc gaagcagtaa acgcatcgga ttcattgatg
    16081 aagttttcca aaaccatgga gtttgctaac tttgggaagt cacagataga aagctcgaaa
    16141 aaagaaatga aagactacgc cgataagacg gtttatggtt tagaagaaat tctgaacaca
    16201 accgcacaat tggcatctaa tgggattcct aactatacag aactaaccaa ggcggcaggt
    16261 aacttgaatg ccgttgcagg cggttctagt gatacattca aatccgttgc catgatgcta
    16321 acgcagacgg caggagctgg gaaactaaca actgaaaact ggaatcaatt agcagatgcg
    16381 ataccgggtg cttcaggatt gttacaagac gctatgttga aaaacggagc ttatacagga
    16441 aacttccgtg atgcaatggc gcaaggtcaa atcacttccg acgagttcaa ccaagcaatt
    16501 gtacagttag gtatgaatga cggagcagtt aaggcagcca cttctacaga cacattgagc
    16561 ggttcttggg agcagatgaa atccactgta ataaatgggc tacaaagtat tatagaaaaa
    16621 ataggcgttg aaaatatcac tggtttcatc aacagagtaa caaaagggat tgaaaattct
    16681 attcctaaaa ttactcaatt tataggttgg ttgagagata ttggaacgtg gatcgttgaa
    16741 aatagagagc cactaacatg gattgtcgga atcataggcg gaattacatt agcagtaaaa
    16801 gcattgaacg tagcaagtat gttgctggca attactggcg gaacattggc agcccctttt
    16861 gtggcgattg gtgtagcatt aggcgcacta gcaggtgctt tggtgatagc ttatacaaaa
    16921 tctgaaacat ttagaaatat agtcaatgcg gcttttacag ctgtgaaaaa cgtagttatg
    16981 agcgttatca ataatttggt ggaatactac aaaatgttgt ggagcgtgtt gcagtggctt
    17041 tgggaaaaaa taaaagaatg ggcttcatgg attggcaata aattcattga aatgaagaac
    17101 agcgttgtga atacggttca gaacctatgg aatggcgtga aaaacttctt cagcaatggc
    17161 gttggagata cgtggaataa ggtagtcggt tgggtaaaaa acattttcaa caaagcaact
    17221 gaattgaaga acaacgtttc tgatgtaatc ggtaacctgt ggaatggtat caaagacaca
    17281 ttccgtagag gtatcgatac ggtattcaat tggttttcag aactaccaac gaagatgaag
    17341 aatgccatta ttggcggtaa aaacgccatt gttgatgcgt tcaaaagtat tttcaacgca
    17401 gcacttaaag cgataggtaa accagttaac gcaatcatcc atggagcttc atgggtacta
    17461 gaaaaattgg gcgcagaacc tctacaagaa tgggatgtac cacaatacgc tacaggtaca
    17521 ccagcaggtg ggcacccaat caatggtcca atgatggtta atgatggacg tggagcagaa
    17581 acagttatca caccagatgg tagagccttc attcctaaag gacgcaatgt tgttctaaac
    17641 gcaccaaaag gaacgcatgt cttgacagca gaagagaccg cgcaacttca aggttctaaa
    17701 gctcctaagt atcgttacaa aaaaggtact aacttctttg gtaacatgtg ggatagtgtg
    17761 aaaaaggttg ctggtaatgt aggtcacaca attaaaaacg tagtaggtga cgtgtgggac
    17821 tttatttcag acccgggagc gttagctaga aaagtacttg gggggttaga tgtattaggt
    17881 ggattgacaa agtatccatt agaagtaggt aaaggcatcc tatctaaagc aacaagtgca
    17941 ctgactgaaa aaatcactgg gttgttctca tctggtaact tagatacctc cgtaggaaca
    18001 aatggcgtct ataaatattt ggctgatgtt gctaagtctg tgatgaagaa attcccagga
    18061 tttgtggcaa ctagtgggta tagaccaggt gacccttatt cacatggtaa acgtaatgcc
    18121 attgatattg cattaccagg cgtcacagga ggctcaccta gatacacaga agcagcaaac
    18181 tacgcttttg acaaattcgc ttccaaaatt ggttacgtaa tcactaatgg gaaagttcgt
    18241 gaccgttcag gacaatcagg tcaaccagca actggtgcat gggagccatg gcccgatgga
    18301 gatcactatg atcatgtgca tttaaacggt gtgaaagacc cacaaaacac tcaaatttca
    18361 ggagatagcg tgggaggcag tggggtagaa agatggcgca atgtagcaat tagagcgttg
    18421 aaaatgaccg gtcaatacag tactgcaaac ttaaatgcat tactaaatca aatgcgtaca
    18481 gagtcaaatg gtaatcctaa tgcagttaac aattgggata ttaacgccaa aaatggaaca
    18541 ccatcaaaag ggttgctcca agtgattgac ccaacattca gacagtatgc aatgccagga
    18601 ttcaacagca atatttttga cccactatct aacatcttag cttcaatcag atacgcacta
    18661 tcaagatatg gctcactaac aaatgcctat cgtggagttg gttacgcaaa cggtggaatt
    18721 gtaaaccaac atcaaattgc ggaaatcgca gaaggaaaca agccagaaat tattattccg
    18781 ttagataagg ctaaacgatc aagagcgatg caattattgt ctatcgcaat ggacaaacta
    18841 ggagtttctc ctaaaactta tggaaatgcg acaactgttt ccagtgatta cgaaatccta
    18901 gaagccattg aaaaacaggc ggcaacaacg aatcagctgt tatcgttatt gctggcattt
    18961 ttcaaaggaa atagccgaac tgatagagat ttagctttag atattcagaa gatcttggtt
    19021 aggaggatgt agatgcgaac tgtattactg agaaatagaa taaacgaaga aattgatttg
    19081 tccacagaag actattttgc tactgggttg agtaatatgg ggtttgaagt aaaaaaagaa
    19141 catgtgggac agtgggggaa tttcagagaa agcagtgaga gcgttgaaat atctgaattt
    19201 cagtcatctg taatcatttc tgtgcatggg tttcgagaaa aagaactgta caattcactt
    19261 gtgcagttcc tatcggaagg tccgtttgaa ctggaatttg cttttgacgg cgaaacaatg
    19321 gtaagaagat gcagtctaaa atctttaagt aagactgaaa tcgatccgaa aacatcgtta
    19381 ctaacagaca ctttagaact atatttcaca tcaaactggt attccgtaaa acgcgaaaaa
    19441 ttaattcagc gaccgaatgt agtgaaaaca cgtggtaagg tttttccgta taaaagatct
    19501 tatatctaca ctcaaaactt gtgggagaaa aaaggtgtat tcaaattcaa taataattct
    19561 gtatacctaa cgaatagcaa ggaacggatg tccccgctaa aaattcgtgt gattgggaag
    19621 tgttcaaatc cgtattggga agtaatccaa aactcacaaa tcatcgcaac ggatggatac
    19681 ttcatagata tgactgaaac acagactcta gaagtatcaa gcctttttga agatacaacg
    19741 gcgattttaa aagatattgc aggggtagaa tcttctgtct accaacaaca ggattataca
    19801 aaaactaatt ttgttcaggc cccaactgga gaattcagca ttgtgtttca tgttgggggg
    19861 gcagacgtag agattgagtt atacgaggag cgtgatctgt tttgatttta gcagtcacgc
    19921 tttttcatcg tgatttaatg ctttataacg aacatttatt ctctaacagt tttgaatttg
    19981 gtgtggacga gatcaacgaa gaggctagta gctttacgat ggataagtac gttcctgtaa
    20041 aaacaggcga ttttctacta gcaaaatata ttcctagtgg aaaatttgca tattttggtg
    20101 taattacgtc gcaggaagac gaaaaaatca gttgtaaaag cttacttagt ttagctgata
    20161 gtgagatacc gactgcacgt gtgtcaggag ataactacga agagcatatc cggcggttga
    20221 ttgaatacta tttgctgaat gatccaacga aacaactaaa agatatttta gacgtcaaag
    20281 cagaaagtgc gacctctcat tcgtatcaag ctaccgatac gaataaacac aagttaagtg
    20341 cgtatattct caatggtttc aaaaaataca acgtaaaatg gtatttcaag ggaattcaaa
    20401 acagaaaaat ttatacaggc atacgtgctg taaatgaatc aatttacatt aaagacaatt
    20461 cttctgaatt tagcgattgg gatgtgtttg ttcaggcgcc gggcgctgga aacgagaaca
    20521 agctattaat cgttgataaa gcaatgaaag atatagagaa accgataata ctgtcaacat
    20581 ggtatttgga cgaagaaaac aatttgacac aagatggatc gaaagaaaat atcacgaaac
    20641 caactgtcaa tttggttaat atctacgatc aaaccgcaga agataaagca tcctacgaag
    20701 atgtggcaaa atcagagttg aaaggcaata cgtattcgca tgaaatcaaa gtgaacgttg
    20761 taagaaatgc aaaaaatttg aatgtcgaaa cgattgaaac agggatgttt gccacgattt
    20821 cttataaagg aaagatatac aagtcggttt tgacagcttg gcgaatatca agtgataagg
    20881 agtttgtgga attgactttc ggaaacataa gaagtcgttt tatggattat tttgaagata
    20941 atgggggata aaaaatggtt agcaatgtgg atggatatca atttgaaaac gtgaaagtaa
    21001 gcgcagaaaa tgatgctaga ctttatcacg ttttatataa tcggaaaaat caggttattg
    21061 atggttacga tcagtctatg aatttatctt caagcggatt aacagtaaaa gttgctgcag
    21121 gagcagcgat tattcaaggt cgtatggtcg ttgttcgaca agaagaaagt ataacagttc
    21181 cagcaaactc aagtggttat atagcattaa cagtggattt gacacaagaa gttatacctg
    21241 gatctattct tccagaatcg gaagaatatg aatggactaa taatcaagtc aagctagaat
    21301 ttataacaaa agttataaaa ggtaatttaa acaatggtga taaggtctat aatttaccac
    21361 tatgctcagt taattctact ggatcaactg tttcaatctc aaagatatcg gatagttacg
    21421 agctgactct ctctaaagga gaaattttgt ggagggggac tgcgttaatg catgatactc
    21481 aaactgtcca accttcaaaa aaaatttggc agacagtttc aggttttttg ttagtatggc
    21541 tcccatacga aaacgggcaa gcaattgagg atagatatgt aactacgcct ttttataagg
    21601 agcgtgtaac ttttactaat gttttaggag aaattgtttc aggatttgat gactatcaca
    21661 aaaagtggtt tagtaaacga ataaactata attcgaatac taatatattt acaggtgcag
    21721 caagcaatgc aagtggagat aacgcaaata tggtgcttag gtatatagtt tctttttagt
    21781 taggaggtat ggaagagtgg caaatttaga aattaaatta tctgcaaata agagacagcc
    21841 ttatctacgt caccgtgttg ttggtagagt tggtgatggg gggctcacaa caatcaatgt
    21901 acaacttctt gaggaagatg aaattacacc ttttgtaatt aatctaaacg gtactttgaa
    21961 atttgtgggc gaagtttcaa acggtaacta taccgaggga gaaccagaaa taatcgattc
    22021 gactaatggg ttaattagtt acacgttcac taagtcaaat ttcagcacga gtcatcaatt
    22081 caaacaagca tattttgaat atgtagatcc taacggcaaa aaagtaactt ttcagaactt
    22141 catcatagac gtgttagaac gagtggatat taattcggag caagcgaaat actatatttc
    22201 ttcattggaa aaattacaga gtgaaatgca aaccactttc aatcagttca ttagtgataa
    22261 acaggtccaa tacgatcaaa tctactcgaa atataacgaa ttagtaagat tgataaatga
    22321 atcagataag caagtaaatg atcgtattga tcaaaccaat cagcaaatcg gcgatctcgg
    22381 caagctgaaa aagatgtaca gtaacagcat cgactttggg ggctatgatt attcggggaa
    22441 tccgaatttg ttaagtaagc tatcatacga cttaattgaa aatcaaaata cttcagctgg
    22501 aacactttct aagggtgaaa actcgtttaa atataataag atatcagctg aagtggaagg
    22561 tggagtagag ttatattata aacgaagagg tatagctaac tggttaccct ctaataaaac
    22621 gcttgtaatg actgttaagc ttagagctgg agcggactat agtccagttg acggaaaact
    22681 tatactgatt agatataggt atgttgacag tggaactggc aagattgttt tagacttacc
    22741 tattaacagt aattcgataa ctcaggaatg gaaagagttt agtattactg gaactactcc
    22801 aacatttagc ccacaagcat accatccttg gatacaattt agggctcaag atggggtact
    22861 tggggaaata gaaatgagct atgacatcaa aatcgaagaa ggctctacag ctacaccata
    22921 tcagcctaac ttacttgatg actcttactg gctaggtaaa acaccgttgg gtgagaattt
    22981 gataacgaat gccaagttcc caattataac acgaagcaat cctatttctg gttttgatat
    23041 ttcagaagaa ttaataatag gtgaaactta tacggtatct ttaaaaggga ctaagccagc
    23101 tactaaagaa ttccacttat actacggaga agcaaattac cagcagttgc agtatcaggc
    23161 aactttactt cctgtggaag gattaacaga tgtgtggagt gccactttta cagccagaaa
    23221 tactagtgaa acgacaaatt tacttagagt agcgttatgg caaaaaccta attctgcaac
    23281 atatgatact gttcaaattg actggctcaa gattgaaaaa ggcgacacac gcacaccaaa
    23341 tatcagtcag tttaaatact ttggtgaagg cttgaaagac agtaacaatc cgaacgacta
    23401 cagctgggat gtcacaccag agtatactga aaaaagcttg aataatacgg ttagtctgac
    23461 tgaaccgcaa tccatcgaag gattgaaaaa tttcgaagat ggtattcaat caaaaggaaa
    23521 atctgtattg acatcagacg acaacaaata tgaagtcgta accttaacag ttacaaacgg
    23581 gaataccgga tcagcaaagc tttatcgtga aggaaaaacc gtcactattt atttttttgc
    23641 gttgaatggg aaaagcagtg gtggaaatga ttcaacgata ctaacaattc cagaaggcta
    23701 tcggccacca attagttttg agcaactggt tggctcgata gaccgttcta ctttgaacag
    23761 tgctcagtta tctattggtg cagatggagc cattaaatgg cgaagaaact caagttatgg
    23821 atcggattat acctttgcaa ttacttacac gatttagaaa ggcgtgaatc gatatgaagg
    23881 cagcatatag accaattgaa ccttacggat tcgagcaaat cattgtgaat gatgaagaac
    23941 atttaccgga agaatgcaca gaagtcgaac caccgattcc aaattggaaa ccgaaattca
    24001 attactggga gggaaataaa tgaaaaacat ttggaaatat ggccgtactg gcggagagta
    24061 tgcaggaaaa gtattggacg acatgcttgt atccgttcct tacacagatc agccaccgct
    24121 cgaaggggtt cgttcagatg gcgaaccgct aacgattgct gatcagatgt ttgatcctaa
    24181 attgaaccaa tggattattt tagcgaacgc gttagatcac aacgatttaa acaatctcaa
    24241 agcgatgtac gaggctcttg aacatgaaaa cggcaaccta aaacagctaa atgccaaact
    24301 catgctaagc gatgtagcga ttaaacagga aaatactgca ttgaaagaaa aagctgacag
    24361 tttagcacaa atcaattcaa aaatgatgct tgcttcgtta caaaatagca aagacatttc
    24421 agaaattaaa gagcaactaa atccagcttc aaagggaggt gagtagtatg tttagtttta
    24481 gcgatgtgaa aatgatgtat gattggggct gttttactga cgatcaagtt cgtctattcg
    24541 ttccactatg tatcacagat gaagaagcag atagaattat tagcaaagaa gagagcgcat
    24601 cttaagtgat gcgttttttt gttggaaagt tggtggaaca tgaaagaaga agcgctccaa
    24661 gacgttgtgg agagattagt aagaattgaa acaaaattag acaactacga atcacttaga
    24721 gaaaaagctg atagtgcaaa agatttggca gacaaagcct actcagtagc gctaaacaac
    24781 gcagaagata tcaaagaaat gaaagccaat aataaatggt cgtggggtta catgatcggt
    24841 ttaggcatta cgatcattgg ctatttcttg actaaattgt aaaggagtta agaagaaatg
    24901 attttacccg ataagtatta tcaagtcatt aaatggacgg ttttaacagt attaccagct
    24961 gcatctgttt tagtagccac gttaggaaaa gcgtatggat ggaatggaac agatatgaca
    25021 gtactcacta tcaatgcagt agcaacattt ttaggcgtta tcactggtgt gtctgcatat
    25081 aacttaaaaa aataggagga aaaaaatgaa gaagaaaatc attttatcat tgagcctaat
    25141 aatggctctt tttttattgc caattaacgg gttcgcctat acgatcaaca atgaatttaa
    25201 tttaggtgta aatgaaggta gctcacaagt agcaaataac caatacatcc tattgcatga
    25261 aacagcaaac gaaactgcga caggacgaaa tgaagcgcag tacatgaaac gttcttggta
    25321 caatgcctat acagcgtata ttgttggcga tggtgggatc gtttaccaag ttggacaacc
    25381 tggttatgta caatatggcg ctggttcata tgctaatgca aatagtcctg tgcaaatcga
    25441 actgcaacat acgcacgata aagtaacgtt tgaaaagaac tacaaggcat atgttgaatt
    25501 agcgagagat tccgctaata aatacggtat tccacttaca ttagacacac cgtataatca
    25561 accaggaatc aaatcacatt tatgggtaac tcaaaacatt tggggcgatc acactgatcc
    25621 gtatggatac ttatcagaaa tgggtgtaag taaggaaaaa ctggcttacg atttagctca
    25681 tggatttaca gacgaaaacc caacgacttc tgagaacaag cctgtcattg atccaacccg
    25741 agcaggtgca gcaaatccaa ctttatcaga tggaacgaac cattctcaca ttgatcagtt
    25801 tggagaaatc gaaaatgcga acttacacgt cgctggatgg cacatcgcta actataaata
    25861 cgagtatatt ttcattatgg actacaatac tgggaaagaa ttagctcgag taagagctga
    25921 tggaatttat agaccggatg taaatcaagc ttataatact tctggaaacg ttggctatca
    25981 tgtatctttt aacatgcgca atttccctaa taagaaagta tacgtcatga tgcgggcaac
    26041 gaatgatcca gaaggaaaca ctaaaggcgg tgcgcaagat ttccatgaca aacgttggta
    26101 tttaaatatt cctaaacgat aaaaatagct cctcgttgag gagcagtaca tagaattgaa
    26161 aattaacgtt aaatcaaaaa aatatttaca agatgtttat ttaagtagat aataaaagaa
    26221 aaaagcaggt gaaattgtga cagcagaaat tggtattatg aataaaagtg gaatcgtgtt
    26281 agcttcagat tcagcatcta cgattggaga tagcaaagta tataatactg caaaaaaatt
    26341 atttactttg gattccatgc attctgtagg tatcatgata tatggtaatg ctgaatttaa
    26401 tggtattcct tgggaaataa tcattactca atataagaaa agcattggta gttctgtttt
    26461 taatactttg gaagaatacg cggataattt tatagaattt gtcaaaacag catcttttat
    26521 tagaagtgaa cagaccgagc aagaacaaat gattggggtt tttcaaaaaa taatttcagg
    26581 tttatttgag agtatagaag tagatattaa ctttttaata agccaaggta cacaaataga
    26641 caaagatgtc ctagtgaagt tattacaaac aaaaatgaat accaatttgt ctcaacagtc
    26701 ccagacattc attttagata ttgaaaaggc gttatttctt agtaactatg gagaaatatt
    26761 aaaagatatt cttaatagta tttctacaat ggaaggtgtt tcagaagcta tatcagagga
    26821 aattcaaagt tacgtttatg aaattataat tagagacgat gtttattctt cccctacagg
    26881 aatagttata gcaggatatg gaagaatgga tattttccct aaactatatt cttataatat
    26941 gtttggattt gttatgaata ttctgaaata ctcagaatat gaaagtgctc aaataggaaa
    27001 tgacaatggt tcattaagat ctactatatt accatttgct cagtctgatg tggtaaatac
    27061 tgtagttcaa ggggtagatc cacaaattac taattatctg tcgagtcaag tcgatagttt
    27121 tgacgataac ggaaaaaata cctatattaa tattataaag aatatttcag agtttcaaca
    27181 aaatcaattc atatttccgt tactaaacat gattgcttta ctcccagttg aagaaacagc
    27241 aattatcgca gagactttat taaatttaac cagttttaaa cgaaaatata ctacttctgt
    27301 agaaacagta ggtggcccaa ttgatgtttt agctattaca cctaatgatg gccctatttg
    27361 gattaaaaga aaacattatt ttgatattga taataatatt ggatacagat tgcgaaagga
    27421 gagagcgaat gattacaata attaaacctc aggaaaaatc ccctgttttg aaatttaatg
    27481 agagcaatct agcccataat aaaatgagta taactgatac agtatttgat gtaatggttg
    27541 agcaacaaaa aaaaattaat aataacaata gacaaagaaa agaaattaac caatattaat
    27601 gttttttctg ttgcgttttt tgcagtcata atttaaaacc tcgctcaatc ttgggcgagg
    27661 tcttttttta tttttctgaa aagttaaagt aaataatagc ataaaataat atattttaca
    27721 aagaataagt acaatctagt tttttgctat taaatgtgta ataataaata tgccatcaca
    27781 acatgaagaa tgaaatccat tattatctag tctagtccat tctttttgtt tgcagtattt
    27841 gtgatggctt tc
//
LOCUS       DANKBF010000035        27668 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000035 DANKBF010000000
VERSION     DANKBF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            238..2004
                     /locus_tag="PWY89_001598"
     CDS             238..2004
                     /locus_tag="PWY89_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL2066641.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2106..3365
                     /locus_tag="PWY89_001599"
     CDS             2106..3365
                     /locus_tag="PWY89_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2066642.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3512..4813
                     /locus_tag="PWY89_001600"
     CDS             3512..4813
                     /locus_tag="PWY89_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2066643.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4816..5667
                     /locus_tag="PWY89_001601"
     CDS             4816..5667
                     /locus_tag="PWY89_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2066644.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5680..6495
                     /locus_tag="PWY89_001602"
     CDS             5680..6495
                     /locus_tag="PWY89_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL2066645.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6646..7812
                     /locus_tag="PWY89_001603"
     CDS             6646..7812
                     /locus_tag="PWY89_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2066646.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8042..8689
                     /locus_tag="PWY89_001604"
     CDS             8042..8689
                     /locus_tag="PWY89_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2066647.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8773..9717
                     /locus_tag="PWY89_001605"
     CDS             8773..9717
                     /locus_tag="PWY89_001605"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL2066648.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9852..11303
                     /locus_tag="PWY89_001606"
     CDS             9852..11303
                     /locus_tag="PWY89_001606"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2066649.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11324..12160
                     /locus_tag="PWY89_001607"
     CDS             11324..12160
                     /locus_tag="PWY89_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2066650.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12160..12819
                     /locus_tag="PWY89_001608"
     CDS             12160..12819
                     /locus_tag="PWY89_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2066651.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12945..13634
                     /locus_tag="PWY89_001609"
     CDS             12945..13634
                     /locus_tag="PWY89_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066652.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13640..14596
                     /locus_tag="PWY89_001610"
     CDS             13640..14596
                     /locus_tag="PWY89_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2066653.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14640..15152
                     /locus_tag="PWY89_001611"
     CDS             14640..15152
                     /locus_tag="PWY89_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2066654.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15408..15956)
                     /locus_tag="PWY89_001612"
     CDS             complement(15408..15956)
                     /locus_tag="PWY89_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2066655.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16045..17226)
                     /locus_tag="PWY89_001613"
     CDS             complement(16045..17226)
                     /locus_tag="PWY89_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2066656.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17462..18970)
                     /locus_tag="PWY89_001614"
     CDS             complement(17462..18970)
                     /locus_tag="PWY89_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL2066657.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19129..19320
                     /locus_tag="PWY89_001615"
     CDS             19129..19320
                     /locus_tag="PWY89_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066658.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19523..20440
                     /locus_tag="PWY89_001616"
     CDS             19523..20440
                     /locus_tag="PWY89_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL2066659.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20433..21506
                     /locus_tag="PWY89_001617"
     CDS             20433..21506
                     /locus_tag="PWY89_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066660.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21654..22172
                     /locus_tag="PWY89_001618"
     CDS             21654..22172
                     /locus_tag="PWY89_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066661.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22153..22545
                     /locus_tag="PWY89_001619"
     CDS             22153..22545
                     /locus_tag="PWY89_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL2066662.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22621..23556
                     /locus_tag="PWY89_001620"
     CDS             22621..23556
                     /locus_tag="PWY89_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2066663.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23689..24468
                     /locus_tag="PWY89_001621"
     CDS             23689..24468
                     /locus_tag="PWY89_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2066664.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24461..24778
                     /locus_tag="PWY89_001622"
     CDS             24461..24778
                     /locus_tag="PWY89_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2066665.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25022..26632
                     /locus_tag="PWY89_001623"
     CDS             25022..26632
                     /locus_tag="PWY89_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2066666.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26789..27637
                     /locus_tag="PWY89_001624"
     CDS             26789..27637
                     /locus_tag="PWY89_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2066667.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8960 a         4891 c         5553 g         8264 t
ORIGIN      
        1 aaaactttta ctttagaaaa ttggagaaat tgatcgctta agataaattc aggacactaa
       61 atagtacagg aagtagatta aaacttttaa aagttttaag tctatctatt tggcaagttt
      121 aaaaaataaa aattcggtat caaaaaactg ttaccgataa catttttgaa agcgttgtct
      181 tttgtttctc tatcacttat agtgatgacg tcatattaaa acacaaggag gaaatcaatg
      241 gatacagctg caatttatca tcgaccggaa agtgaatatg catttctata tacgaataca
      301 agatttcgta ttcggcttcg tactaggaaa aatgatatca aaaaagtata tgtgctttgt
      361 ggagatccat ataccataac aactgaaaaa tggtatcaaa aacaacgacc aatgaaaaaa
      421 tgtctaagta ccaatgttca tgattattgg gaaattgaag tgacgagcga aacaaggcgc
      481 ttacagtatg cgttccatgt tgttggggaa gataacacgg actgttttta cggtgatcaa
      541 ggaatttttc cttatcgaga aaatgtgatt actgaaccaa atttttattt tcgaatacct
      601 tattttcatc aaatcgatcg attcactatt ccagaatggg taaaagaaac agtatggtat
      661 caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa atgaccccga acacacttta
      721 ccttggggaa gtaaagatcc agaccgagaa gacttcttcg ggggagattt gcaaggtgtg
      781 agggatcatt tggattactt gacggacctt ggcgtcaatg gcatctattt ttgtcccata
      841 tttaaagcca catccaatca taaatacgat acgattgatt attatgaggt ggatcccgct
      901 tttggcgata aaaaactttt aaaaaacttg attgatgaag cacataaaag agggatacgt
      961 atcatgttcg atgctgtatt caatcatatg ggtgtccatt caccacagtg gcaagacgtt
     1021 ctcaaaaacg gagaaaaatc gatctataaa gactggttcc acatccgttt tttcccagta
     1081 gattcttatc agatgactga cttaccagaa actgctgaaa atatcccata tgatactttt
     1141 gcttttactc cattcatgcc gaaactaaat acagcaaatc cagaagtgca aaaatatttg
     1201 ctggatatcg caacttattg gataaaagaa ttcgatattg atggatggcg tcttgatgtt
     1261 gctaatgaag tcgatcatca tttttggaaa aaattcagag aagcagttac agaaatcaaa
     1321 ccagacatct atatccttgg agagatttgg cattcctctc aagcttggct gcaaggggat
     1381 gaattccatg cagtgatgaa ttatgcattc acagattcga ttaaagatta ttttgcaaaa
     1441 aagaaaatca cagcttcaca aatggtcagt ggaatgaatc atcagcaaat gctttacaga
     1501 gatcaggtga acgaaggaac attcaactta ttagattctc atgatactgc caggatcttg
     1561 accctttgcc aaggtaacaa agaattgatg aagtctgtga tggcctttat gtttcttcaa
     1621 aaagggtctc cttgtatcta ttacgggaca gaaataggga tgactggtga agatgacccg
     1681 gattgccgca aatgtatgat atgggagaag gaggaacaag atctagagct attcggtttt
     1741 ataaaagaac ttgtttctct aaggaaacag ttgtctaaga ttatttctga aggttctact
     1801 cagtggctaa tagtatatga tcgtgaagat aagctctatt tcacacggga actagaggga
     1861 cagatcatct atgtttactt taatcagtca aaagaacctt gggtagtaga acaggaaaat
     1921 gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg ggaaagcaga gatacgacaa
     1981 tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa caacaagaaa gcgtttgcaa
     2041 atgaagattt gtgcccatta taataagggt gtaacagcac gttgaataca caggaggaaa
     2101 tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg gttaatctct ttaggaatct
     2161 taggcggatt agcggcttgt ggcaatggta actcttcagc agaaggggaa gccgatgaag
     2221 gaaaaacgtt aacagtttcc gttgattctg gatataaaga ttatataaac gaaataaaaa
     2281 acactttcga aaaagaaaac gatgtgaaaa tcaagctagt agaaaaggac atgttcgatc
     2341 agttggaatc attagcgtta gatgggcctg ctggaaaggg acctgacgtt atgatggcag
     2401 catatgaccg gatcggtgcg ttaggacagc aaggacatct ggcggaagta aaactgggaa
     2461 atgaagcagc ttacgatgaa acagacaaag cccaagtgac ttatgatgga aaaatctacg
     2521 gagaacctgc agtaatcgag acccttgttt tgtactataa caaagattta gtcgatacag
     2581 caccatcaac gtttaaagat ttggagaatc tatccaaaga cagtcgtttt gcttttgagt
     2641 cagaagcagg aaaaaataca ggatttttag ctaaatggac tgacttttat tattcctatg
     2701 gtttaattgc aggttacggt ggctatgtat ttggtgatga tggcacaaat ccttcagata
     2761 tcggtttgaa taatgcaggt gctgtagaag gaatctctta tgcaacagat tggttcaaaa
     2821 atgtttggcc aaagggaatg caagacataa aaagtgcagg agacttcgcc agccagcaat
     2881 tcatgtccaa taaaactgca gcaatcattg acggtccatg gcaagcgcaa acatacaaag
     2941 aaaacaatat caattatggt gtagccaaaa ttcctactct aaataatgga cagccttatc
     3001 aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta tgctaaaaac aaagacctct
     3061 ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca agaaaaattc tttgaaatgg
     3121 taaatgaaat ccctgcaaac caacaagcta gggaaacagc aaaggctaaa aacgatgaat
     3181 taacaacagc tgtgattgaa caatatgaaa cagctcaagc gatgccaaac atcccagaaa
     3241 tgggcgaagt ttggacaggt gcagaaaact tgatgttcga tgcagcatca ggttcaaaaa
     3301 caccaaaaga atccgcagat gaagcagtaa aaacaatcag tgaagccata gaacaaaaat
     3361 actgattaaa atgataaccg tccattttta taaaaaatgg aacaggtgct tcagataaaa
     3421 gtggcaagag cgagatggaa aattatcttg ctcttgttgc cattatctta aaagcagttt
     3481 gcggtttagc aagtttagag ggggcattca gatgcagcaa gataaaacaa gcaatatcaa
     3541 cgaaaaaaaa atcacgcttt gttcacttat tccagggatg gggcagtttg taaatggcca
     3601 agcttttaaa ggtatcgttt ttttcgccat attcgtccta ttcattggtc agatgcttct
     3661 gggcggattt catgcattgg taggattggt tactttggga agtataccaa tggaagatca
     3721 ttcattattt attttgattg aaggcacttt gcaattcatc attacaatca ttttcttgat
     3781 tttttacggt ttgaatcttt tagacgctta tcgagtagcg aagatgcgtc aagagggcaa
     3841 aaaaatcaat cggacgatga aggaagtcat acataatata ggtgatgaag gttttcctta
     3901 tctattgaca cttcccgctt atcttctcat gatttttacg atcattttcc cagtcctagt
     3961 tacattgttc actgcattta ctaattatga cttcaatcat attccaccag ccagtttaat
     4021 tgattgggta ggggctaaaa acttttttag tatcttcttt ttaagctcat atcgtaatac
     4081 tttttttgca gtcttttcgt ggaccgtgat ttggacttta tgtgcaacaa ctttgcaaat
     4141 cacccttggt attctcacag cggttgttgc tcaccagaaa tttattaaat tcaaacgtat
     4201 tttcggtatc attttcttat taccatgggc ggtcccagct tttattacta tcatgagttt
     4261 ttcgaatata ttcaacgata gcgtaggtgc catcaatact caagtgattc ctttcataaa
     4321 ccagcttcct tttgtagata tcagtgcaat ctcttggaaa acggacccga actggacgaa
     4381 agtagcaatt atcttagtgc agggctggtt aggatttccc tatatctatg tgatggttac
     4441 aggaatctta caatcgattt cagaagactt atatgaagca gcaaaaatcg acggagcaaa
     4501 tgcatggcag cgcttttcaa acatcacgct tcctgctgtc ttcctagtgg cagcgccaac
     4561 atttattaca cagtatacgg ggaatttcaa taatttctcc atgatctatc tgtttaacaa
     4621 tggtggtccc ggaagcgtag gcggaggagc tggttctact gatattctta tttcctggat
     4681 ctataaactt acgactggaa cttctccgca gtattctatg gcttcagcaa tcacgttgat
     4741 tatctccgtc atcgttatct gtgtgtcatt gctggtattt aaaaagacaa atgcgttcaa
     4801 tatgggggat taacaatgaa aaaatttcat tcgagtgctc gattccaaaa acgactttct
     4861 cactttttta cttacttttt tcttttaata ctgtcagtca ttatcatcta tccgcttttg
     4921 atcacagcaa gtactgcctt caagtcagga aatattgcag cttttagttt agatctttca
     4981 agtgactgga ctttaggaaa ctttactcgg ctattcagtg aaacacttta cgggacttgg
     5041 tataaaaata cattgatcat ttctgtccta acaatgatcg tacaagtgac tgtagtaact
     5101 ttggcgggct atacttatag tcgctatcgt tttatcggcc gcaaatcaag tttgatgttc
     5161 tttttagtga ttcagatggt accgactatg gcagcattga ctgcctttta tgtcatggct
     5221 ttcttattag aagcgctcga taagtactgg ttcttgacaa tgatctatat aggcggtgga
     5281 attccaatga atacttggct gatgaaagga tactttgaca ctgtaccaat agatttagat
     5341 gaatctgcta aacttgatgg ggcagggcat ttcaggattt ttttccagat tgtattaccg
     5401 cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt ttatgggacc ttttggggat
     5461 ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc agactgttgc tgtcggctta
     5521 caaacattta tttctgatgt gacaaaccaa agagtctctc tttttgctgc tggggcaatt
     5581 ttgattgcac taccgatttg tctattattc ttctttttac aaaagaactt tgttaccggc
     5641 ctgacatctg gcggaacaaa aggataggag cagagtatca tgcaaacatt tccgttaaac
     5701 tatttttccg cattacgtac gccaactcaa gtgtttgcgg gacgcaaact attatcatgg
     5761 cctaaatttt tcttgatatt tgtattttta gtttccttga tggtcatgcc tgtgactctt
     5821 ttttatgcca atcagatcca agcgattcca ttagaacaat ttctatccgt tcattcgctg
     5881 atcgatgaac aaggaaccca aaaattttct gaacttgaat tatctgaaac tgggttacaa
     5941 gcatcgcaac aaacgatagc tgtgacccct gaaattttgg taggtgtgtc tttatctgag
     6001 aaacaacaat ccgatcatgg aacttttatt gattttgaga aggaacaatg ggtgatccaa
     6061 caaaaggata aatctggtat acgccgctat acgatgaatt atagcccatc tttccaacca
     6121 gattctgtac gtacgcccga agatttccaa cgatttttag aaagagaatt ttatgccagt
     6181 aatcgtccaa caatcatttt atcttatagt ttaagtcttg gtcttgtcct atttgtcatg
     6241 acgagtctca ttttattcgg ggcttccttt ttcttgtgga tgacaagaaa aagccaactt
     6301 tcatccattc atacattcaa agagagtgca aatttgatgc tgaatgttat gggaataggt
     6361 tccatggcag cagcagttgt agggtttatc cactttgact ttatcttgat gctaagtgtc
     6421 caaacaacga taactgtctt gctgctttta tgggtttttg tcaaaacaag attcaaagat
     6481 aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa tgaatgtgaa aaaaatgaaa
     6541 aataaagtag tttttgaaaa atttctgaaa aaattaaact tcttagttga ttcttttctt
     6601 ttttagtatt aacgtaatag ggtagttttt ttaaggagga aaatcatgtt agagaaaact
     6661 ttatacaatc aattattttc acgttctttc tcgctccctg tcgaagtaaa ttattgggac
     6721 ggtacaacga aacaatacgg agatacggac actcctcccc aaatcaaaat caatatccat
     6781 gaagaaatcc caatgaaaga gattacaagt aacgcttctt tggcactagg agaagcctat
     6841 atggatcatc gaatcgagat cgagggaagt atccaagcat tgatcaacga tgcttatcag
     6901 caagcaaaca gctttttacg aggaaaagat tatctaaaat ggctgccaaa gaaagaaaaa
     6961 catacgaaac aaaaaaacaa agaagatatc catgcgcatt atgacttagg taacgacttt
     7021 tataaacttt ggctagatcc aactttgact tattcttgtg cttatttcaa tacacctgaa
     7081 gatacgttgg aacaagcaca aatcaataaa gtccatcata tcttagacaa actctttatc
     7141 aaagaaggcg aaacgttgct tgatatcggt tgtggctggg gaacattgat gttcactgct
     7201 accaaagagt ataatgtcaa ggcaactggg atcacactaa gtgaagaaca atacgatttg
     7261 attaccaaaa gaataaaaga agaacattta gaagataaat gccgtgtatt attaatggat
     7321 taccgcgaac taaaaggcga aacgtttgat catatcacaa gtgtaggtat gttcgaacat
     7381 gtaggttctg aaaatcttga aggttatttc aaagtggtaa aagatttatt gaaaccaaaa
     7441 ggaacagctt tgatccatgg aatcagccga caacaaggcg gtgcgacaaa tgcttggatc
     7501 aataaatata ttttccctgg cggttatgtc cctggtgtta ctgaattagt tgataatatt
     7561 acaaaaaacg atttgcaact gattgatttg gaaagcttac gtcgtgacta ccaattgaca
     7621 ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc aagtcacgaa agaaatgggt
     7681 gattccttct atcgaatgtg ggatctatat ttacaagctt gtgcagcttc atttgaatca
     7741 agtaatatcg atgtgattca atatttgctt gttcaacctg gaaacaatga catcccaatg
     7801 catcgtgcat aattgaggca aaagtactgt ttaaagacta tttaaaaaga agctaagttt
     7861 tatgatgctc cagaagtgaa cttttttgtc taacttctag gattgtctct aaagcttagt
     7921 ttctttttct ttatattttc ataataaaag tttggttctt taatttttaa acatgacaga
     7981 aaattttttc ttcgttatac ttgtaataga atactatttt ataacaaagg ggatcgaatc
     8041 aatgaaaaaa actgttattt atactagttt atttgcagct ttgtttttag ctgcttgtca
     8101 atcaaatacc aatcaaggca catctgggaa tacgtcttcc agtagcgaac agcgctcttc
     8161 acaggtatcg gactcctcaa caaaagcaag cacatcttcg tctagccaga caaaaagttc
     8221 tagtgaagca agtactcagt cagatatcaa agtgtctgca gctcgtgcga tcgaattgtt
     8281 ccaagataaa tacccagata ctgcaattac tagtttggaa ttagatagtg attggggatc
     8341 ttacttctac aagatagaag gtgtcgatga tcaaaaagaa tacgaagcaa gaattgatgc
     8401 tcaaaatgaa aaaatggaga cagagaatcc agaaactcta gatcgagatg aacaaaatgg
     8461 tcaaaagaaa aaagaagatg gtctggatgt tgataaattg atcacgattg aggaagcagc
     8521 aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca gattgggatt tggataaaga
     8581 tttaggtact acttattggg aagtcaaagt aaaagacggc aatcaaagta catcagtcaa
     8641 gatcaatagc caaacaggcg aagtactgtc aactgaacaa gatgattaag aatttcttcg
     8701 gaaaacaaga gttgattttt cttttagtca tgatacacta gacaaaataa cttgtttgaa
     8761 ggagcgattt acttgagttt agaaacgacc tttgccaatc atatctttgc caatcctttg
     8821 atgaatgcat ctggtgtcca ctgcatgact acccaggaat tagatgaact ggctcattca
     8881 gaagcaggag catttatcac taagagctgt acgatcaatg aacgaaaagg aaatcccgaa
     8941 ccacgctatt ttgatgttcc tttagggagt atcaattcta tgggacttcc aaacttaggt
     9001 ttttcttatt atctagagta tgctcttgcg tatgaaaaag tgcaggaaaa ccaaaatcaa
     9061 ccgttatttt tttccattgc cgggatgagt gtccaggaaa acttggaaat gcttgagaaa
     9121 atagaaaaaa gtgggtttaa tgggatcact gaattaaatt tatcttgtcc caatgtgcct
     9181 ggaaaaccac aactagctta cgattttgag gccacttatg aaacactgaa agaagtattc
     9241 agcatctttt ctaagccgct aggaatcaaa ttgcctcctt attttgattt tgctcatttt
     9301 gatcagatgg ctgatatctt gaatcagttt ccattgacct atgtcaatgc tatcaacagt
     9361 gtgggcaatg gtttgtatat cgatacagaa caagaagcag tagtcatcaa accaaaagaa
     9421 gggttcggcg gcattggcgg agagtatatc aagccgactg ctttggcgaa tgttcgcgct
     9481 ttttatacac gtttgaaacc agaaatccaa atcattggta caggtgggat tcggacagga
     9541 caagatgcat ttgaacatct gctatgtggc gcaagcatgt tacagattgg tacagagctt
     9601 cataaagaag ggccggaaat tttttctaga atcataaaag aattaacgca gattatgtca
     9661 gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat tacgcaccat ttcatagcaa
     9721 agagacgaag gtcatgattg atcttcgtct ttttttataa aaaacactcg tctgttcatt
     9781 cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg ggaaaataac agaggcaaaa
     9841 aggaggataa tatgaatgat gcaacagaat tagcaaaaga gcttaaagaa aaagaattta
     9901 gttttcaaga actagttacg gatatacaga aaaaaataaa gaagcaaaat catgaattga
     9961 atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca cgagctattg attccccagt
    10021 cagaaaaagg gccattggct ggtatcccat ttcctttaaa aatgctcggt caagaaaaaa
    10081 agggatggct agctacttca ggatctcgtt tatttgaaac acaccgagct tctcatacat
    10141 ctaattatgt gcggcaagca gaagcgatag gtctagttcc ttttggacaa accaatgcgc
    10201 cagagtttgg tttcaaaaat attacagacc cagtcatcta tggcccagca agaaaccctt
    10261 ggaaccttga atgtacaccg ggcggatcaa gtggaggagc tgctgctgca gtttcatcag
    10321 gaatcgttcc tttagctggt gctagtgatg gcggtggttc tattagaata cccgcttcgt
    10381 tttgcggatt gattgggctg aaaccttcgc gagggacgat gccagttggt ccatatgctt
    10441 ggagaggatg gcaaggagct gctattgatt tcggcttgac agtttctatg agagacacgg
    10501 aggctttatt ttatgggatg agaagtatcc attccggtgc gccatatcaa gtatcgccag
    10561 ttgagtggca aacacatcca agaaaaaaac ggttgaagat agctttttgt gttgattcac
    10621 cgatccactc acagattagt gaagaggcag tacttgctgt aaaaaaagct gtagatttcc
    10681 ttggaaaaga agggcatgat ttgacagaga ttccttaccc actagatggt cgtaaactga
    10741 tcacgtctta ttatcagatg aatggcgcag aaacagcagc aatgatccgc tcgattgaaa
    10801 caggcatcca gcgagctgtc agaaaagaag aacttgaacc catttcttgg actttgttcc
    10861 aatatggtca gaagctctct gccgctactt acatcgattc tctgcatgta tgggatgaag
    10921 cagcaattgt catggaaaac ctacatcaag aatacgatct attcctttca ccaacgactg
    10981 catttaccgc ccctaaaatc agcgaagatt tgcaaagtga tgcgataaga gcacagatgg
    11041 aacacatcag tgattgttct gaagtagaag gtttagacgt tgtttcttcc atgtttgagg
    11101 agagcttgaa gatcacacct tatacacagt tggctaatct gactggacaa ccagcaatca
    11161 gcttgcctac tcatttgaca aaagaaaaca tgcctctagg aatccaattt atggctgcca
    11221 gaggaagaga agatttgcta tttcaaatcg gctatctatt tgaagcaaat catcagttcc
    11281 atttaccaaa tccatggaaa tagcagaaac aaggagaaga agaatgaaga aaaaaagatt
    11341 gtttgctcta ataactagtg ccttttttat tttaagcgct tgcggtacag ctgccgctaa
    11401 tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa gaattagtag tagcgacttc
    11461 tggcacactg tttcccgcat cttattataa tgatcaaaat gaattagttg gttatgatgt
    11521 agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa atctccttta aagaatacaa
    11581 cgtggatgga caaatcactt ctgtttcaaa aagagaatca gattttgctg cgaacgattt
    11641 tggtatcaca aaagaaagag aaaaaaaatt ctccctttca gagactttca aatattcatt
    11701 tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc cactcacttg aagatcttaa
    11761 agggaaaaaa gcagctggcg aacccaatac tagctatatg cgtttagcgg aaaaacacgg
    11821 agctaaatta gtcacatatg acaatgccac gaatgatcag tatttaagcg atgtagcaaa
    11881 tggtcgaaca gatgttattc taaatgatta ttatctgcag aaaatggccg tatctgcttt
    11941 accagatatc cctgtaaaaa tactggagaa cgtttatttc aatgaaaacg aaagtggttt
    12001 tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc gatggcgtat taaaagagat
    12061 gaaagaagat gggacgacaa agaaaatatc tgaaaaatat tttcaaactg atgtttcagt
    12121 caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga tgtactttcc cctaattgat
    12181 ttttccctca tgtggacttc acttcctttc gtactgcaag gcttggtata cacattaggg
    12241 attggcttcg tgtcgtttct tttaggaaat ttggtagggt tgcttttgac agttttaggt
    12301 cttttggatt ggctgcctct gaatgtattt attcgttttt atttatcgtt ctttagaggc
    12361 attcccgcac tagtcctgct gtttctgtta tatttcgggt taccttatca attaagtgcc
    12421 ttaacggctt cagtcatctg ctttacgatt accagcagtg catttattgg tgaaatctat
    12481 cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg ctggtttagc ttctggtttt
    12541 tctttctttg gggtattgaa atatatcgtt ttgccgcaag cttttcgaat ctcagtaccg
    12601 gcactcagta acgtagctat ggatctagtg aaaggtactt cgttagcagc aacaatcaca
    12661 gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc ggacattcga ttatttttct
    12721 ctctatattt tagttgcact gatttattgg gtcatttgta tcgggatcga aaaagtgcaa
    12781 cagtatttag aaaaacgttc tatccatcag tcaatctgaa aaatagaaat ggtgctagcg
    12841 tggctggcac catttctatt ttttgtttct tgtatattct gcctagtatt ttaaaaccat
    12901 ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga aaacatgaag accccgctaa
    12961 ccatcgaaat agaatcctcg ttataccaat attgcatcga gcaaggcggc ttggttgtag
    13021 aagaagtcac gatgcctgat gaccaaggaa ttgtagatac gcttagctgc ttgacaaaat
    13081 ttgatggaag ccgagaatgg cgatgctatg agctgaaagt gacaaaagca gattttcgct
    13141 ctacagccaa actgtcattt gttggacatt ataattattt tgttttacca agtaaactat
    13201 atgaagaagt aaaaactgaa atccctacag gaataggtgt tttagtgtat cgtccgtaca
    13261 cggttgaaga ggagatgcct gcctctggta catttctgat tgcgaaaaaa gcagcaaaga
    13321 aagagcttgc tgtaccggaa gaagctttga caaaccgctt catggcttct ttattcaggg
    13381 aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata ttttccaaaa gatcaattgt
    13441 acaaagaatt gaaaagacgt tcagaaaatc aagatccatt ttctaacgaa cgatattatg
    13501 aacggttttt agaggaaata gctagtcagg caatcaatga gttgcaagaa gaagttatcg
    13561 cgcttcagca tgattatgaa tatttgaggc aacaacagca gatccgtcgg ttgcctacag
    13621 agccattgga gtgaaaaata tgtactatcc ttatttaaga ggaaaacaat ttgacctatt
    13681 agcattaaaa gaagcactgt cgcgaggatt gctctccaat aaaatccaac cagtgatcga
    13741 accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa ttatttcaaa aaaagcacca
    13801 tccaatcgct gtgatccaaa atccccaagt aggacaattc aaattattcg atcagcatgt
    13861 gcacagttgg acagtcaaag aaaatagctc tgtcgtccct gcacagatct taacgccaga
    13921 aaatctcagt gaagtactgg attcacctcc agcgtttctt gttttcgatg gacagcacta
    13981 cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt gattcgaaat ttttgatacc
    14041 agatactagt cgctttcgca tctggctgcc cgaaaacaaa attgtgatcc gtgactcttt
    14101 tcaaacgaga aagcatgttg aaagttatgc agataaaaca gatgactttt ttagtgatga
    14161 ctatcttttt ttccacgcag acggatatat tggattttct gatttcacga tcgaaggtag
    14221 ccgttatttc gataaagggt tccctagccg agcgcttgct atccatctaa cgtatattga
    14281 tgcttacggc aatatacgcg tgaaacattt tgtctctgac agtaacgact ctgctaaaga
    14341 ccaagcattg aagtttcttg aagcaggaga taaaatgaaa aaatggatca tgcgtcacca
    14401 tcatcaactc ttgattactt caggaatgat tgaattattg gctctttacc agcaacaaaa
    14461 attccctgga ctgggagtgt tgaaaaagtg gtcacttatg catcatttgg aacttattag
    14521 tcacctgcta gatcatccaa aagattggct taaatattat tcaaaagttc aggaactata
    14581 tgaagtgatc caatgattag ataatgtaaa tgaaaaagga atagaaaaga ggaaactgga
    14641 tgtatattcg gcaaacaagg aaagaagaag tagaagaagt ggtagaaatc attaatgccg
    14701 cagcggattt cattgcttcg caaggctctc ctcaatggca aaacgggcag aaaccaacaa
    14761 aagaaacgat tttgaacgat attgaaagaa aagaaagcta tgtattaata gtggaagatc
    14821 aaattgctgg aacagctgca ttagtctcag gcgtggatcc cgtttacaca aaaatagatg
    14881 gtagttggaa agaatcaact ggtccttatt tatcgattca tcgtgtcgct ttagcagaaa
    14941 gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca tttgttgacg attgcagcca
    15001 gcaaaaatat ccaagatatc cgtatcgata cttatccagc taatgaaccg atggaaaaga
    15061 ccatctattc tgctggattc acttatcaag ggatgattga atttcctttt gaacacggcg
    15121 aaagaaaagc atatcaaata atcatcaatt gactataaaa tcatctgagc aaagataaag
    15181 acattaacac tactgaaaaa aacaatcata tgatagccaa gaggttgtaa cagaagtggc
    15241 cagcttcaaa aaacaagaaa gaatttccga aaatgcttct caaatttttg tggaatttta
    15301 gcgtatttta cgaaatagct gctttctgaa actcccttca tcgagaggtc gtgacatagc
    15361 ttttgtcacg atctctcggc ttatcctacg agtattattc gatacattta tatcaataat
    15421 ccttttttta ttttatctgc agcttcttgg aacgctccat cgtttgacac gactaaaagt
    15481 gaggggatag ctttcaaacg tttcaactgt gctaaatctt ctacataaac cgccagtctg
    15541 gctgaaatct gttcagatga atctccacgt tttttcaggc gcgattgcaa tgtttcttta
    15601 gagatcgtaa taaagatagg acggactacc gtcccgaaat actcatataa atgccaaaaa
    15661 ccatttgctg tcaaaacggc gtaacaattt cctactgtca gtttcttttt aatttctgct
    15721 ttactggaac catatttatg ttgagcatat tggtcccact ctgcaaactc atttcccttg
    15781 atcatctgat tgaatgtatt gtcagaaaca aaataataat cctcttgatc tttttcccca
    15841 ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt ccggctgaaa aatagcttct
    15901 gcgagtgaag tttttccaga accagaagga ccgataaata catagcaata attcattgtc
    15961 atttctcctt tgatgattga acaagtgaaa aagctttgga aaatatttcc aaagctttta
    16021 aaagtaactt tgcttttttg tattttatac cagcaagcca gtcacaatag cacttaaaaa
    16081 gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt tttgcgacaa gatttccttt
    16141 ttcttcattt aatcccttca tcgcaccaga aatgatcccg atagaagaga agttggcaaa
    16201 tgaaacgagg aagacagata taattgctgt tgtacgctca gtaaaatgga aattaccatt
    16261 tgctaattct gtcatagcaa caaattcatt tgtaaccagt ttagtcgcca tgatactacc
    16321 ggcatccact gcttctttcc atgggattcc gctgatgaag gcaagagggg caaagatgta
    16381 acctaggatc tcttggaaag tgatgccgaa tatcccgtgg aagatagcat tgatcatggc
    16441 aatcaaagca acaaaaccga taagcatagc agcaaccgtg atcgccacat ggaaaccatc
    16501 aagaatgtat tctcccaaca tttggaaaaa tgtctgttct ttgttttctt caatgatcag
    16561 ctcatcttct ttttcttcta atacataggg atttacaatt gaagccagaa tataaccgcc
    16621 aaataagttc aaaacaaggg cagtgatcac atatttagaa tcgatcagtg ccatataaga
    16681 tccgacgatc gacatagaaa ctgtagacat agccgataca ctcatggtga acaaacgttt
    16741 ctcagatagg aagggtaatt ccttttttat ggaaataaaa acttccgatt gtcctaaaat
    16801 agcagaagct actccattat aagattccaa tttgcccatc ccgttgattt tacttaatcc
    16861 tagaccaaca gctttcataa ataatggaag aatcttgata tagcgtagaa taccgataat
    16921 agcagaaata aatacaattg gcatcaatac ggaaagaaag aaaggtgcac ttcctttatt
    16981 tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca aaagcaagaa gatgatcaaa
    17041 catttttgcc aatacagaaa caaccgctgt accaaaagtt gtacgtaata atacgaaacc
    17101 aagaacaagc tgtaaaacga acatcacaat tagtggtttc aatttgattg cttttttgtt
    17161 actgctgatc aaaaaagcaa gccctaatgt gacaaataaa ccaataatac ctaataaata
    17221 actcaaataa tcactcctat tccaaattcc gttttatttt acctgttttg caaataaaat
    17281 actaatcttt agtgaaattt tcattacttt tttagagtga aacgcattca ttttccatta
    17341 gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa cgtcttagta aagtctataa
    17401 aaaaacgaaa ctatcgatta agctaatagt ttcgttttca acatttataa gtaaactttt
    17461 tctattggta atatagattt tcggaagggt agacaggtgc gcaggtaata tcgattccta
    17521 gttttttcaa tgtttgttca tcatttttat tgaccatcac agtagagtgg gcttgtgctc
    17581 cctcaagttc agctagttta tcataagcaa cttgagcgat tggattcgtt actgcactga
    17641 ttgataaagc gatcaagact tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct
    17701 ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga caataaagga atgtcatctg
    17761 cgatatgggc taaatgtttt actgcattta agataactgc cgctgacgca tccatcaatg
    17821 aacttgtacg cccggtaatg atgacttcat ctggtaattc gattgcaatc acagcagaag
    17881 gatcttttgt aggactttgc gcttgaatct tatctgcata gtcatgggca gggactacca
    17941 cgtttcggtc ttcaggacgt aaaccgactt cttccataat cagtttgatt cgattaactg
    18001 cttcttcgtc gatcaatcct tttttgaagt cgcattctgt ttggaaataa cgacggatga
    18061 tttcttgttt agaggcttct tggacaacgg tatcatccac gatcccaaat cctacacgat
    18121 tcacacccat gtcagtagga gattgataaa cagattcttt accagtaatt ttttcaataa
    18181 tacgtttgat tacaggaaat gtctcaacat cccgattata gttcacagct actttattgt
    18241 aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt tagatcgact gtggctgctt
    18301 cataagcaat gttcagcgga tgtttcaacg gaacattcca tactgggaaa gtttcgaact
    18361 tggagtaacc agctgcatgt cctttttggc tttcatgata cagttggttc aagcaagtag
    18421 cgagctttcc acttcctggg ccaggagctg tcactacaac gattggttta gttgtttcaa
    18481 tatactcatt ttttccaaaa ccatcttctg agacgatttt ttctacgttg actggatagt
    18541 cttcgatttc tgagtgttta tagaccttga tgtttcgtct ttctagttta ttgataaaga
    18601 ctttcgtggc aggctgtcca ctatagcgag tgataacgac actgttgatt aataagccat
    18661 aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc ataagtaatc ccataatcac
    18721 cacggatctt attacgttca atatctccag catagacgca aatcagtatt tcagcttgat
    18781 ctttcaattt ttgcagcaat ttgattttcg catcttcttc aaatcctgga agtacacgtt
    18841 tcgcatgctt gtcgtcaact aacttcccac caaattctaa atataatttg tcatagtggt
    18901 tcactcgttc caaaatatac gctgactgct cttcaatata tttttctgaa tcaaaaccaa
    18961 tttttttcac taggttccct ccttaagtac taaagcattt tttcattttt ttgaggattc
    19021 gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa atggtttaaa ccaacttgtc
    19081 tagatagtta ttcattggta tgatagtttt tgataaggaa ggtgctacat gaaaaaaaga
    19141 cagaaaaaga aaaacgcata taaacactat atccgttcga tttttaccgg ttatgaaaga
    19201 atgcttgaag atcccgaatt ggaacaattg acgtttactt atctaaacga agaaacgcag
    19261 ctaactcgtg atgagcatca gcgcattcat ttcacaacaa gagatctgcc tagtaaataa
    19321 aaaataagat aaaggtcatc ggattcgatt ctgtcctatc agatggaaag actagttgat
    19381 cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt atggagcatt gcccgctctt
    19441 ttttataaca attttttaca gaaaatggta ctattgaagt attaataagt gaaaaacaga
    19501 tgagtaagag gaggcaagta gtatgaaaga gccagataca aatgcaaatt atgagccaga
    19561 cgaactagag gagacgaacg cggatcagga tacaggacaa gaatcgtctt ctttttacga
    19621 tatcaaagaa aaagcaagca tgaatctttc ccaattatta gaagacttac aggcttttga
    19681 aaaagctgtc cgcgaagaag atatccctga gatctaccgt ttatacaatg gccgattgaa
    19741 tgaagaatta aaaagaagct caaatgaaaa tcacgagatc gatcaattgc tgatgcgtaa
    19801 gatccatgat cgttttttac aggaatttcc ttttatggaa caagtagaaa acatctcgcc
    19861 aacgatgagc tattacaaaa tcggcactta ttaccatgat cggcccacgg tcggtattga
    19921 tgcaagtctg ccagaaattt ttgttttgcc taagatcgat gaagagtggg agaaatattc
    19981 ccaaccggag tcaacggact atgacaaaaa aatcaatgag ctagacgcaa aggtgatcac
    20041 tgctcagaca gaaattgaac gtttagatga acaaataaaa gaaatcaatc gacaaatgac
    20101 tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa atcgaggaag aaattcaaga
    20161 attagagaag aagaagcaag tactatctaa tgaaaaatta ggctggcttc cttatattga
    20221 gacgcctgaa acaatccagc agcaaaaaga agcattgaag caagaagcaa gagcagacca
    20281 gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc agacgctatt tcggtagcaa
    20341 agagggattc ggtcaagcaa tccatgaatt cttgatgaat tatcttggca atgagaatcc
    20401 taataatcaa tctaatgaag gaggttccga gtatgaatga acacgatgaa gcagaatgga
    20461 caaaaaatga agaacaaaca gaagatcatg aagaaacgaa cacttcattg gaagaacaaa
    20521 caagtaatcc tctggatgac acagaagaaa tccctatgct agaggaaaca aaaatacatg
    20581 aaaaagaaaa agtaactccg acgaatacga atactgcaga aaacacccat gcttacgaaa
    20641 taactgaaaa tcccgggtat ttagaaggaa aagagcaaga tcatcaagat atcgtcagtt
    20701 tgattgaaac agcggaagca gaattagcgg atttacgtat gagaaaagat ttgatcgaat
    20761 cagattttcc acaattatta gaatcatatg atcgttttat attgaatgaa gaaccaatct
    20821 ccacgatcgg aaagacaagc ctgatcgaat acttcacgta tgaattactg aaaccgttga
    20881 atgatattgg tctggaacgg tcaaattcag cttatgacac atggaaaaca agacattttt
    20941 cgatcaatta tacgttgaat ggacaaaacg aattagtctt ttcctttgta ttaccgacca
    21001 tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa cgtatcacca gagacaatgg
    21061 aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg acactggtca gtggatcgag
    21121 tattctctgc agggcagatc acgatattca atcacaaatt gaatcaatta ttagcccatg
    21181 caagaacact tggttttacc gttaatcaga cattactaga caatacgaag ccacttcgtt
    21241 tgaatcttca gagcgaattt gaattgacga atgaagtatt ggatgatatt tttatcgtgg
    21301 caatgaaaaa tccatcctac gatatggaaa agatcaacga aactacttat caagtacgat
    21361 tagataaagg acagtcttta acgataacaa caaacgaaaa agatcaaaca gtcttgtcga
    21421 tctcatctgg agactaccca cgatctgtca ttgatttctt tatcaattac gaattcttgg
    21481 tcccattaat ggtgagaaag tcctgattgg ataagttgga ttaccagatg tcattcagat
    21541 aattctgttc caaaggactt gcggcagtta tccagaataa ttgtcacaag tcttttctgt
    21601 atgtactaaa tggacgtgaa taacttgctg atgaaggagg gatttcatta ggaatgtcca
    21661 caaataaaat agcacagctt agcttattgt ctgctgcttg tgtagcaggt aggatcgctt
    21721 ttcaatttat tccgaatttc cagccaatga ctgctatttt tttatttata gtactttact
    21781 taagcttaaa agatgcgtta attgtcatga gtctttctat tgcgatcagc agtttttatt
    21841 ttggtgttgg accatgggtc atcggccaat ggatcagcta tactgttgtt ctgagtttgt
    21901 tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct atggttaaag ggagtctgtt
    21961 tttttctggc gggcatggtt tacggaatag gtatgaccgt ttttgatacg ctgttatatc
    22021 gtttgcctca accttggatc tattaccttc aaggggtgtc gtttgatttg atgcacagta
    22081 taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt caaacgtttt tacaatcatt
    22141 caggaggaaa aaatgaaaaa aataatttat agtttgcttg caatgagtgt cagtatactc
    22201 attttgacaa gttgcagtac aacctataca caatcggctt ccaaccaaaa ggaggaagta
    22261 aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg gtcaaaactt ttcggaaaaa
    22321 gaagtggtct tcaaaaaagg cgatgattta ctgagtattt tgaaacgaaa ttttgaagtc
    22381 aaagaagata acggcttcat cacttcgctt gaagggcact cacaggatga gaaaaatcaa
    22441 ctctattgga tgtttactgt ggacgggaaa agtgctacta ctggtgccaa agagatcaaa
    22501 ttgaatgatg gacaatcggt cattttcaat ttgtctaaac tataagtttc ataaaaaact
    22561 gttaaagggc gaatattgcc ataattcagt gagttcagtt gatgaaagaa ggaaaataac
    22621 atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg ctgctctagg ggctaatatt
    22681 ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg gcagtgccgc catgctaaac
    22741 gaaagcatcc acagtatcgt tgattgcagc aatcagattt tgctgttatt cggtgataaa
    22801 caagccaata aaggacagag cgagttgcat caatttggtg aaggacgagc aaaatacttt
    22861 ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg gggcacttgg ggtgatggaa
    22921 gcttttgaaa agttgacgca tccatcccat gaagtgggta atccgatcat tgttatagca
    22981 atactgttat ttggtttggt aatcgaagga agttcgttac gcgtggcaat gaaagaaatc
    23041 aaagaattga atactgaaaa actttccacg atgcgctttt tgcgcgaaag tcgacacagt
    23101 gaaatactga ttatttttac ggaagacttg tgtgctgtaa tcggtttggt cttggcttta
    23161 gcaggaacaa tattgactta tgcaaccggg attgctgcat ttgatgcgat cagcggattg
    23221 ctgatcggtt tcttgctgat gggagccgcc attttcctag caaaagaatt ttatagtctg
    23281 atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa tcaagttagc ttttgaacgt
    23341 cctgagatca gtcggctgat cgatataaaa acagttcatt taagtccaac ggagatttta
    23401 gttgcagcaa aagcagatat cgttggtaac aaagagaatg aagcttactc ggtcatcaat
    23461 gatattgaaa aaaatatgcg tcagtctctt cctgacaaga agatgtatat atacattgaa
    23521 acagataaat atgacccaaa ttattctcgt aaataaaacg tttattcatg aattgaagca
    23581 aaaatgagaa aaatataatc ttgtttttaa ataaaacggc tacaatttag cgaatttatg
    23641 ttacaatgag tccgctgttt atttgttgta attaaaggag atattttaat gaacaaaaag
    23701 aaaagtgcgc catggcttca agctttgcat gcagtgatgc cactttgtat aagctatata
    23761 ccagtaggat tagcttgcgg agtattgttg caaaaagttg gatttgatcc tctcttatca
    23821 ggactgttat cgattctggt cttttccggt ggcgcgcagt ttttagttgc atctatgctt
    23881 acgacgcaag catcatttgc aacgacatta ttgatggttt tctttttaga attacgctat
    23941 acattattag gagcaagtct tgccggtttt atgaaaaaag aaaagcggac gtttttggct
    24001 gtcttttctc aatcactgaa tgatgaaaac tatgcggtga actacttaaa gttttcaact
    24061 gacccttcct ggaataaaag aaaggcttta tatgtcaatt ggttctcaat gactgcatgg
    24121 gccggtagca atatgatcgg taattttttc ggctcggtga ttcgagtaga tgctgacctt
    24181 gtccattttg ctttgacggc catgttcatt ttcatgttta tcatgcagat gaaaagcgca
    24241 ctactgatct ttacaggttt attctcaggc gtattagggg tcgtattcat ggtattattc
    24301 caaaatactt tcggattgat tgcagcaaca gtcattgctt catttgccgg atttatgctg
    24361 gagaaggcat tcaaaaaaga aaaagctaag aaactaagag accgcggtaa agatgtcaat
    24421 gagccgctct tcaacttccc ggaacaggag gccaatcacc atgactaaac tatatttgct
    24481 acttttagtt gcttgtctgt tcatcgtggc atatatcccg cgcttgttcc cgatgcttta
    24541 ttttactcat cgaaaagtgc catcctggtt cagtgactgg atgaaatatg taccggtggc
    24601 attatttgcg gcgttagctt ttaaagatgt tttcattact catgaacatc tggatattgc
    24661 ttggaatatc aaaatagctg caatgattct agtagcaggc gtggcttaca aaacacgttc
    24721 gatggcgttg tcagttttga caggacttgc gtctgtcttt ttgctgtcta tgctttaaac
    24781 tgttttattt tattaaaaac agagattgcg acataagttg ttcaaactga tctatccgaa
    24841 ctgcatttgc aacagtcaat tctggatgca agtttattcg tagggataaa aaagattagt
    24901 gttggcttat gatgcaagct cttttttatt ttaggaatta gagagggatt tgtccttcta
    24961 tgattttttg ctataatagg gaagatgttt attttatgaa agaatagttg agggaattta
    25021 aatgaataaa aaattgatgc aaggaacatt ttggctaacc tttgctaatc ttctatgtaa
    25081 agttttaggg gtagtctatt tgattccctg gctaagtatg atggggaaca atcaagatgg
    25141 tatgcttgct acgacattgt ataatgttgg ctatcttcct tatggattgt tcctgatgct
    25201 tggtacagtc ggatttccaa atgcgattgc aaaaaaagta gctgtagcca caaaagaagg
    25261 agataacgct gcttgtcggc tgatttttag aagtacgatc aatatcatgt ttgtcatcgg
    25321 tatcgcctct gctgcactga tgtatttatt tgctccttta ctagccggaa tcagtccgat
    25381 tgctaacgtt aataatggga tactagctat cagaagttta tgcccatctt tgattgctat
    25441 cccaattctt agtgcaatga gaggatattt ccaaggaaaa aatgatctgc ggccttatgg
    25501 aacttctttg attatcgaac aggctgtgcg agtgatcgtt atcttggctg gtacttatta
    25561 cttgcgcgta ttgactgacg gaaccatatt agaagctgta ttgatcagta cagtcgcttc
    25621 tttcttcggt gggttagctg cgattatcca tatgtatatc gtcggacgca gaaaagactt
    25681 cttcgaactt aaagattttt tgatttccag ccgttatttc gaaagagaaa accgatctgc
    25741 ctctgtctct atcattcggg aaacactacc gtttattttt gtcggctcag tcattactat
    25801 cgttcaaatg atcgaccaag taacgatgaa accactgctt cactttttcc gtccagagat
    25861 cgcagatcag caactggaat tcttattcag ccgtgcttct gtcaatccaa ataaattgac
    25921 attgatcctg atctccatgg taggaacagt agctataagc agcttgccaa tattaagcac
    25981 gatgaagaaa aaagatcgga tacaaatcga aaaaacagta ggagatagtt tttcgattgc
    26041 gttattgatt cttcttcctt cattgactgg aatgtcttta ttagctggac cactatacac
    26101 attgtttttt ggctatgatc ctgaaagtgt cggatatttc caaatggctt tgctagcttc
    26161 tttgtttttc tctttattca cgatcctatc tacgatgctt caatctttga atcaccatcg
    26221 ttttgcgatc cgattaactg tagaagcaat tatcctgaaa gtcatcttcc aagtaatcgg
    26281 attaggatta gttggcggat acgggatgag cttatcaaat ggcttagcat tcggtattgt
    26341 ctttgttcgc ggctatcact ttatgtgcaa agaataccga atagcacctt tagcaaagat
    26401 cagcaatttt ttcttaaaaa ctttccgctc caccctgatt atgcttgcag tatgttttgc
    26461 cgttttcttt ttgctgaatc agcgattgtc aatggaatca aaaagctatg caatgattta
    26521 ttgtgtggct gtaggaagta ttggcggctt agtattttta ttttctcaat tcggaaaaaa
    26581 tgggtttcga atgttgaaaa atgttaaaca tcatagcaga agcaaagaat aggttaacta
    26641 atcgtttttt gctgtcactt gaaaaacggc aactcgtatg attacgcttg tcgtttttct
    26701 attttccaat tttagtttca tatctaatct tacttatgat atagttataa ggtctggaaa
    26761 aattctttga aacgaggaaa ttaaacacat gaaacttgca gtagttacag atagttcggc
    26821 atatctgccg gaacgtatca agaaccatcc cgacttattc gttattccaa tccctttgat
    26881 tatggatggg aaaatttata acgaaggaat cgatatcgaa gcggatgaat attatggact
    26941 tttaaaaaac agtaaagatt ttcctactac ttcacaacct gtcgtagggg aagttttggg
    27001 tttatatgag gaattgaaag agaaagggta tgacacgatt ttaagtatcc atctttcttc
    27061 agggatttct ggctttgtca acacattatt tgcgatgaaa gatgacatca gcggagtaac
    27121 ggtcattcct tatgactcta agatcactag tatgccgatg ggacacatgg tagaagctgc
    27181 cttagactta aatgatgaag gtcagtccct tgatgaaata cttgcacata tcgatcgaat
    27241 ccgagataat acctatgctt atctgattgt agatgatttg aacaatttag tacgaggagg
    27301 acgtttgaca aatggtgcag cgctgattgg cagcttatta aaaatcaaac cgatcctgac
    27361 attttctgaa gggaaaattg ttttattcga aaaaatccgt tcaagtaaaa aagcatttgc
    27421 tcgagctgaa gaaattattg gtagaagaaa taacgaaata caaccgccag tcaaacttta
    27481 cgtgatacat gcaaataatt tagaagtagc gattgcggag aaagagaaat tgcagaaaaa
    27541 atatccaaat gcagaaatcg aaattggtca tttcggacca gtcattggta cacatttagg
    27601 tgaaaaagcc atcggcattg ccatttcagc gcaataattc cttttattca aaaaacatac
    27661 caaaaata
//
LOCUS       DANKBF010000036        25955 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000036 DANKBF010000000
VERSION     DANKBF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..157)
                     /locus_tag="PWY89_001625"
     CDS             complement(<1..157)
                     /locus_tag="PWY89_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066668.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKE"
     gene            complement(248..700)
                     /locus_tag="PWY89_001626"
     CDS             complement(248..700)
                     /locus_tag="PWY89_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2066669.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(713..1123)
                     /locus_tag="PWY89_001627"
     CDS             complement(713..1123)
                     /locus_tag="PWY89_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2066670.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1398..1610
                     /locus_tag="PWY89_001628"
     CDS             1398..1610
                     /locus_tag="PWY89_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2066671.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1613..1825
                     /locus_tag="PWY89_001629"
     CDS             1613..1825
                     /locus_tag="PWY89_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066672.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1965..2120
                     /locus_tag="PWY89_001630"
     CDS             1965..2120
                     /locus_tag="PWY89_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066673.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2117..2341)
                     /locus_tag="PWY89_001631"
     CDS             complement(2117..2341)
                     /locus_tag="PWY89_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2066674.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2419..2607
                     /locus_tag="PWY89_001632"
     CDS             2419..2607
                     /locus_tag="PWY89_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066675.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2604..2777
                     /locus_tag="PWY89_001633"
     CDS             2604..2777
                     /locus_tag="PWY89_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066676.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2933..3268
                     /locus_tag="PWY89_001634"
     CDS             2933..3268
                     /locus_tag="PWY89_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066677.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3501..4442
                     /locus_tag="PWY89_001635"
     CDS             3501..4442
                     /locus_tag="PWY89_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL2066678.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4444..5334
                     /locus_tag="PWY89_001636"
     CDS             4444..5334
                     /locus_tag="PWY89_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL2066679.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5377..6177
                     /locus_tag="PWY89_001637"
     CDS             5377..6177
                     /locus_tag="PWY89_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2066680.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6192..7043
                     /locus_tag="PWY89_001638"
     CDS             6192..7043
                     /locus_tag="PWY89_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2066681.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7244..7705
                     /locus_tag="PWY89_001639"
     CDS             7244..7705
                     /locus_tag="PWY89_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2066682.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7731..8183
                     /locus_tag="PWY89_001640"
     CDS             7731..8183
                     /locus_tag="PWY89_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066683.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8289..8483
                     /locus_tag="PWY89_001641"
     CDS             8289..8483
                     /locus_tag="PWY89_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066684.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8514..8807
                     /locus_tag="PWY89_001642"
     CDS             8514..8807
                     /locus_tag="PWY89_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066685.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8837..9277
                     /locus_tag="PWY89_001643"
     CDS             8837..9277
                     /locus_tag="PWY89_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2066686.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            9352..9819
                     /locus_tag="PWY89_001644"
     CDS             9352..9819
                     /locus_tag="PWY89_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDL2066687.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10566..10796
                     /locus_tag="PWY89_001645"
     CDS             10566..10796
                     /locus_tag="PWY89_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066688.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10955..11788
                     /locus_tag="PWY89_001646"
     CDS             10955..11788
                     /locus_tag="PWY89_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDL2066689.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11781..13196
                     /locus_tag="PWY89_001647"
     CDS             11781..13196
                     /locus_tag="PWY89_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2066690.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13208..14737
                     /locus_tag="PWY89_001648"
     CDS             13208..14737
                     /locus_tag="PWY89_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2066691.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14655..15707
                     /locus_tag="PWY89_001649"
     CDS             14655..15707
                     /locus_tag="PWY89_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL2066692.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15709..16026
                     /locus_tag="PWY89_001650"
     CDS             15709..16026
                     /locus_tag="PWY89_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066693.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16153..16776
                     /locus_tag="PWY89_001651"
     CDS             16153..16776
                     /locus_tag="PWY89_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL2066694.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16791..17681
                     /locus_tag="PWY89_001652"
     CDS             16791..17681
                     /locus_tag="PWY89_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HDL2066695.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17704..17919
                     /locus_tag="PWY89_001653"
     CDS             17704..17919
                     /locus_tag="PWY89_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066696.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17931..18272
                     /locus_tag="PWY89_001654"
     CDS             17931..18272
                     /locus_tag="PWY89_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066697.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18272..18643
                     /locus_tag="PWY89_001655"
     CDS             18272..18643
                     /locus_tag="PWY89_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066698.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18636..19031
                     /locus_tag="PWY89_001656"
     CDS             18636..19031
                     /locus_tag="PWY89_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2066699.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19033..19410
                     /locus_tag="PWY89_001657"
     CDS             19033..19410
                     /locus_tag="PWY89_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066700.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19411..20019
                     /locus_tag="PWY89_001658"
     CDS             19411..20019
                     /locus_tag="PWY89_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2066701.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20019..20360
                     /locus_tag="PWY89_001659"
     CDS             20019..20360
                     /locus_tag="PWY89_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066702.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20602..23391
                     /locus_tag="PWY89_001660"
     CDS             20602..23391
                     /locus_tag="PWY89_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL2066703.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23381..24121
                     /locus_tag="PWY89_001661"
     CDS             23381..24121
                     /locus_tag="PWY89_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2066704.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24118..>25955
                     /locus_tag="PWY89_001662"
     CDS             24118..>25955
                     /locus_tag="PWY89_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2066705.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9366 a         3722 c         5271 g         7596 t
ORIGIN      
        1 cttccttact taatataaat ctttctctat catgtaattt atttagtttt tcatcatttt
       61 tttgttctaa ttcataagct tcttttttta ttgtgttagc tttcgacagt ttagtgattc
      121 ctataattaa taaaataagt ccaatcaata ataacattca aaactcctca tttctttgat
      181 ataattattg tagagtttac tcagaaatga ggaaagagtc catgtgaaag catgggcttt
      241 tttattttta atgttgtaat actaaatgtt caagattatg aggaagccct aaatattcaa
      301 taatttggaa ctgactcata tcagataaat cttgttcatc tattgttcta gttaacattt
      361 caattgcaaa catattagct tcagcttcta taccataaat catacaacta aggttgtttg
      421 ctctaaaagc tgctgtgcaa atacctttat ggagtctaac gtgggctatt tcatgccata
      481 atacaaatct tttcatagaa ggtgataggt tttgattaat taatatcaca taacatcttt
      541 tattgtgtat cgtttgtcct aatgttttat tccctaaatc agtttcaatt aaatctatat
      601 cagaatattt gattatttta tatggattaa tcgcatcaga tccaaactct acaaataatt
      661 tttctatttc ttctaatatc cagtatttgt ttaaatgcat accaaagccc acctattgac
      721 gatatttttt aggagtgaat tttttctttg ctttaacttt ggttaaggca agtgcaagtt
      781 gaatggcatc gtataatttt tccttatctt catcgtctaa aggttcacca taataatttg
      841 tttcagcatc tgaattcaag ccttcaagta atttttcagc ttgtaaagca atatcttgtt
      901 tatctttttc tgttaaatca taataatggc gtttatcagt acgtcctaaa agataatcag
      961 ttgatacatt aaagtaatct gcaacttttt gtattttatc tccagatgga gtacttttat
     1021 tccatctaga catgcttcca tttccgaaat ttaactttct ttcaagttct gccaacgtca
     1081 ttgaacgttc ttttaataag atttttatac ggtctaatag cataggatat gcattccttt
     1141 caaaataccg acaaaaaaag tatttttctg caaattatct atttacaaat agagaatttg
     1201 cagttatact agttttgtaa gcaagatatt tacggcatac aaagactaaa agcaataaac
     1261 taatagtctc cccagacatc agtaagctcg ctttttgtgc gctgtatttt gtatgcttac
     1321 ataatagcat atttgcagtt tttgtcaaac ttattagaca aaaatattag aaaatattcg
     1381 ataagggggt atgaagcatg actttaaaac agcaagtatt aattgcttta gtaaagaaag
     1441 gttggagtca aagagagtta gctcgacgaa tgggtatatc aattacttat ttacgagata
     1501 tcttcatcga aaaaagaaaa cctaaggaac gtttaaaaca aatcgaagaa cttttggata
     1561 tcaagttaga agtagattct aatgatcagc catcagaaca ggaggcgtaa aaatggtaca
     1621 acgtttgagc aaagcggagt ttaaagagaa atataagtat tctgaaagta cttatcaaag
     1681 aagaatgaag gaatttaaaa aatcacgttt tagtgagggg tataacgcag ttacttccaa
     1741 agaaataaca attgatgtcg atttatatga acagtttaga acatggcgtt ctaaaaatcg
     1801 atttagagtt agagctgttt gttaatagct aaaaaatcta aattcaccac ttctgaaaag
     1861 aggagttgct gctctccttt caagtttgcc aaagtccacg ttttttcatt ttggcaactc
     1921 ctcttcttgg aggtggtgac ggcgagagac gaaggaggga tgacatgaag gaagaatcaa
     1981 aaatagggat tttgaatgag aacaattaca caagattaat tattgaaaca gaagaaggga
     2041 aaaaagtagc agaagttaca ttgaccgatg caactcctgc tgacggttat cgtattcgat
     2101 taactcctaa atatgattaa ccttttggtg ggtgtggatc gtttccgtga ctatcacggc
     2161 tttgaatttt tccatctctg ccatgaataa atagctcaga tccttgattt ttagaaatct
     2221 ctctagcgat ttttactgct tctgattttg tattagtatg tgcagtcgct ttagaatttc
     2281 cagcaccttt gacattccaa ccaccattgg aattaggtac aacatgttga ttttttccca
     2341 tagttttcac ctccatttct atagaaatat tttatcaaat agaaatggag aaattcaata
     2401 aacgaaagga gaagtgatat ggcatatacc ttgcaacaag aacatcaaat tctcggtttg
     2461 attaaacaac gcaggaaaca attacaagat gaccgtgcag cgcttagaaa agccgatgag
     2521 ctatcagata ggcaagctga actaattgct tctgaacttg aggatttgag aatgctagaa
     2581 ataaaaaata gggagattag attatgaaga agacagacac actttttata ggattcattt
     2641 tggggttatt agtgattgta gcgcaccaaa gtattatcgg gggaagcttg ttcgcagcat
     2701 tgatggtttt aatcaatctg cttgattcaa aagaaaggag caactatggc acgagaagaa
     2761 gcgctaaaaa tcggtaaagt gattgctgat aatcggtggg gaaatagccg ccctattatt
     2821 ttaagcaagc aacatatcga aaagcaaaaa gcatggcaac aaataaaaaa gtgactctgc
     2881 cagccagcaa agagtcacat acaaatttat aaaactagga gaattatatc acatgaataa
     2941 gaaaattgaa aatcttatcg aagaattgaa acgtgaatgc caaaagcaag gtgtttctat
     3001 tatttgtaca gcccaaaaag aaggggaact taaaagtctc gtttatggtg agacaacaga
     3061 aattttactt tgtcttgcta tgcaagaaga acatttagat gaaaatctcc cattgtcagc
     3121 acatattatg cgtagaattg ctgtggatgc ttacgaacaa gcgaaaaatg aagaagaaaa
     3181 tcaaccttca aatcatactt ttgttattaa taacaaggaa gatttagctg atgtgatgac
     3241 tcgtattctc aagggggaat ttcaatgatg aatgaaaaaa caattgatct actcattatt
     3301 ttatttttca cgttagtgat tgtagcgaaa agttatgtaa tctttggctt gttattcgca
     3361 attaccatgc ttgtgctttg cgtgttagca agcagaaaag aggttaaaca tgaacgagtt
     3421 agcaaatctt gacaactatt taactgatcc tgattatgct aaaccaccat atgaagcacc
     3481 aattgatgag gaggatgaag atgagtaaat ctaccttaga aatgagccat caagaatggc
     3541 ttgaagaccg taagaaaggc atcggaggtt cggatgttgg aacaatttta ggattgaata
     3601 aatggaaatc tccttatcaa ctatggctag aaaaaacagg acaagtagta cttgaagaat
     3661 caggaagtga gcccgcttat tggggtaatg ttttagaaga agtggttgct aaagagtttc
     3721 aggaacgtac aggcaaaaaa gtacgcagaa gaaaccaagt atttgaacat ccattacatc
     3781 catttttaag agcaaatatt gatcgggaag tagtgggaga aaatgccatt ctggaatgca
     3841 aaacagccaa tcaatttctc ggtaaagaat gggaaggcga agaggtaccg cttagctatc
     3901 tctgtcaagt tcaacattac atgaacgttc taaacaaaga ctattgttac atcgctgtct
     3961 taatcggtgg tcaaaaattt atctggaagc ggattgaacg agatcaagag ctgatcgata
     4021 caatcactga acaattagta gagttttggg aaacaaacgt tcttggaggt atcgagccaa
     4081 ttattgatgg tagtcaagcg acagctgatt tcttgaaaga gaagtatgca gatgttgaag
     4141 acgttcaaac gacgttacca attcatttcg atgaactagt tgaacagaaa aacgaactca
     4201 aacggactaa gaaagaaatt gaatcagcta tccgtcaagt agacaacgag attatcagcg
     4261 agctaggtaa acgtgaagcc agtatcggta tcactcaaaa aaacatcatc agttggaaat
     4321 ttgtccgtac gagacgtatg aactcgaaga aactagcaga gaaatatcca gatgtcgcaa
     4381 atgatgaaga gatttataac gttactgaat caagaaggct aaccgaaagg gagatcaaat
     4441 aatatggcaa caaatgaatc gttaaaaaat caattggcag caaagccaca gaaacaagtt
     4501 gcaccaggtc aattaggact taaagctttg atgaatacac caacaatgag aaagaagttc
     4561 gaagaggtgc ttcatgacaa tgctaatgct tttatgtcga atgttatgac tcttgtatct
     4621 aatgacagtt atcttgcaga tagtgaaccg atgtctatca tgagtggtgc gttaactgct
     4681 gcaacattaa atcttgggct agataagaat ttaggttatg catatttagt tccattcaat
     4741 agtaaaaaca agcaaacagg aaaatgggaa aagaaagctc aattcatgct tggctataaa
     4801 ggatatatcc aattagccca acgatcaggt aaatacaaag cattaaatgt gatcgaagtt
     4861 tacgaaggag aactaaaaag ctggaaccga ctgacagaag agtttgagtt tgatccaaat
     4921 ggtagaacgt ctgatgaagt cattggatat gttggctatt tcgagttact gaatggattc
     4981 aaaaaaactg tctattggac caaacaagaa atcgaagctc atcggattgc taacaataaa
     5041 gatcgcgata agacaaagtt aagtggtgtg tgggcatctg attacaatgc aatggcacga
     5101 aaaactgttt tgagaaatct tctttctaaa tggggaatct tgtccatcga aatgcaagaa
     5161 gccaccacat cggatgagag agtccaaagg gttcaagaag acggcagcat tattgctgaa
     5221 acagaagttg aagaagatat tcctgaaaga aaagaagcag aggttatatc tgaagaaaat
     5281 gaagatgtac aaactggatt atttgatgca tctaatccgc cgttaaacaa ataatgaggg
     5341 agctttctcc cttgcttttc tagaggagaa atacgaatga acacaggata tgtcaaatta
     5401 catcgaaaag tgatgaattc attcgtttgg accaatcctt atatgtataa attatggaac
     5461 ttgtgtttga tgaaagcgag tcatgagaat cgcaagatac tttttaatgg aaaagagata
     5521 caagtgaaca gcggagaatt cgtcacaggg cgcgatgcta tcacatctga gatgaacgaa
     5581 ggcgtgaaac gtgaacatca agtgaacagc ggttctgtat ggagatggct aaaacaattt
     5641 gaaaaaaacg gaatgttgaa catcaaatca actacgaaat acagcgttat atcaataaaa
     5701 aactggtctt tgtaccaaag cagtgaacaa caaatgaaca tcaaacgcac aacaagtgaa
     5761 caacaagtgc acacaaacaa gaatgaaaag aattataaga atgaaaagaa taataaaaca
     5821 tcatcgtcgc aaccacgcaa aaagcgtgtt tacgacaccg actcagttta ctacattctc
     5881 gcggaggagt tattcaaaca gatttgtcag aatcaggaaa tcaaaaagcc gaatctgcaa
     5941 agttgggcag atgacattcg gaaaatgatc gagatcgaca agcggaccga aaaccaggta
     6001 cggggaatga ttgaatggag tcagcacaat gtgttttggg catcgaatat tttatctgct
     6061 aaaaaattgc gagaaaaata cgacacaatg gcagcgcaag caaatcgtga ttataaaaca
     6121 aaacaaacta aaacgcttga atacgagaaa tttagcacag atgagttgcc tatttgagag
     6181 gaggcgtagg catggaaact gttggggaaa tcatggaaaa gctgatacag aaagtgcttg
     6241 tccaacgtgg cgaatgtcct gaatgcggac agcctttgta tggatggcgt acgaagaacc
     6301 ctgatggctc agaacgttgt aagccaacct gtatgcaatg tgggtataaa gcattgcgag
     6361 ttcaggaaga cttacaaaca gaacgaattt acaacgagag cctgaaagca agagcaatca
     6421 attttttcaa aggtggttct gttgtgccta atcaagcgtt gtttgattgc acattgcaga
     6481 attatcaaat tgtcgatcaa gaaacaagac aagcggttga agtaaccaaa cgctttgtta
     6541 attcagtctt gttaggaaat ccaagtcacc ttgttttaac tggaaaacaa ggaacaggta
     6601 aaagtcacct agcgatggca gcggcttggg aagtcttgaa gcgatcaaac tacgataaaa
     6661 aaatcttatt tatcgggtta caggaaatgc tggatcaaat caaattttcg tacaacaatc
     6721 ctgaactcag aaaaacgatt gagggatcgt tgattgcaga tatcaaaaca gcggatttag
     6781 tcatcattga tgatatcggt tcagaactag gaaaagatgc atcagatagt cgagcgtttg
     6841 gcataaacac gctaaattcg ttcttggatg cacgacagaa cctagcaacg attatcacaa
     6901 cgaacttgct tggtgaagaa ctgaaaaaag cttacggtac gagaacaata tcaagaatgt
     6961 ttgtcaactc tgatggattt acgatggtat tttctcaaac agcagacaag cgcataaaac
     7021 cagtcaaagg agcgatcgca tgaatttgac taaacaagaa aaaagaatct gtcgtcagat
     7081 aatgttgcaa attataggtg atcgtgacat ggacgaatta tctcaacaag agagtcgaat
     7141 ggttcggaag ataagtaagc taattattta tcgggataca gaaagatttc tcactgtttt
     7201 aggtaacgtc tacgagaatc cagagttatt ggaggtagag taattgccaa aaatattaga
     7261 tgcatgttgc ggtagtagat tgttctggtt tgataagaac aattctgacg taacatttat
     7321 ggattgtcgt cagcagtatg aagaattatc aacaggtcat gtgatcaatg ttgatccaga
     7381 tgtggtcgca gactttcgcg atatgccttt tgaagatgat gtgttcgata tggttgtatt
     7441 tgacccgcca cacttaagac atgtcggcga aaactcatgg cttgccaaaa aatatggaaa
     7501 attagacgaa ctttggccag aagacatcag gcaaggtttt gcagagtgta tgcgagtttt
     7561 acgaccttca ggaacattaa tttttaagtg gaacgaggaa cagataccac tttctgatgt
     7621 gttagaggct attggagaac agccattgtt tggtaataaa cgaagcaaaa cacattggct
     7681 tgtgtttatg aaaaaattca gctaacggag gaaacaaagg aggaaaagta atgaagattg
     7741 tattaaataa atgttttggt ggctttgggt tgagccatga agcaaaaatg gaaattttta
     7801 aaagaaaaaa tattgaagta ttcccataca tcaataactt tagctatgac tcagatgatg
     7861 aatacacgag atacacaggc caaaagctag gttcgatgga ttttatctac tatttcaaaa
     7921 aagacccgaa aatcgacaaa gttacaggaa tttatagcga aattgaccga ctgtacggaa
     7981 ttgctgatga ttccagtttt tcaagtgatt caaacagagg tgataaggac ctcgttgctg
     8041 ttgtcgaaaa actaggaagt gaggccagtg gaccatatgc aagtcttaaa gtcgttgata
     8101 tacccgatgg ggctgagtgg gaaataagtg attatgacgg cgttgaaact gcacattatg
     8161 gttttcaaac aggtagtatt tagtccacta atgacaacta aagaggaggc agaaggatga
     8221 gtgaagatga atactttgaa cagtagtcta ttaacgacag agatttttaa ctggaaaggg
     8281 gtgtagtcat ggacgcatcg gaagtagtaa ataaaattaa tagtctcaaa gaggttttcg
     8341 gagacgtcga agtagtgata aacgcacaag gctatgacaa cgaatttttt aagcagatta
     8401 ccgacattag cattcagcaa ggaatggaag acgaagacgg aaactttatc gatgaagtag
     8461 ccattttagt gacttgcgag taagtaattg acagctaaag aggaggaagc ggaatgaaac
     8521 taaaagacgg attttactcc agcggccacg gcatcggcgg tttaatgcta gatatgccga
     8581 caaagaatcc taaaacacgt aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg
     8641 aagcagaagg attcatctat ccatttcgtg gatatgtaga acacgtctat aatcactcag
     8701 caatcattcg cattgaaaac acgatggaat gtgataagtg gttagcgaaa agcaaagaga
     8761 atttagcagt ggtgagattg gtggacatgg aagttataaa atcttaattt tgctttttta
     8821 caactatcct acaacaatga gcgttattcc acaactggaa ggagaagagg caattgtgaa
     8881 cattttagag aatatagata ttaagcaaac aaggaaaaat gctagacgat tactaaaaag
     8941 atacagaaat ttagaacgtt tggtcggacc agtgaaaata gacttttccg tgatgactgt
     9001 taccaaaaat ttgaaattca caattgacag tcaaaacgaa gaaatcattg aagcgataag
     9061 tactcgagat tcggttatcg aagcattaac acggctaagt agaatccatt tccaggtact
     9121 ttattatagt tattgttttc ctaataagat gtcaatgtat caaataggag aaaaattagg
     9181 atattccgat agaactatcg agagaatgaa ggcagtagct ttggttgaat ttgctgaggc
     9241 gtataaatca ggagaactca tttcacgtac aaaataaaaa aagtcggatt cctccgacta
     9301 tgaataatat ttccgacata agtattatat cataattggg ggaatcagag gatggtactt
     9361 ttcgatgtaa agaaatatga gacaccaagc gcgaaagatg ttgatatgga acgcacaaaa
     9421 cataatgtcg ctgtgtttct ttcagcatat ctatcagcta gatgtagagt agggcaacat
     9481 cgagaaccaa aagtgacagc atcttattcc ttggttccac cttctacagc tgatcatata
     9541 tttgaagcag aaagaatgct gatcgataaa gaagaagcac aggaagaatt tgagtatctg
     9601 cataaattgt ttgttcgagg ctattcagcg atacagcatc cgcataagcc tgatgtgact
     9661 gaaaggcgca agaagatatt ctatgatcgt tatatcaatg gtctgcccat ttatgtaact
     9721 gctcaaagga ataatactag cgaagaatcg gttaaagtag aatcaaacag aattatcatc
     9781 caatttgctt catcgttaga actggttgct ttcaagtagc cagtttttac actttttata
     9841 cccttttatt accaatttgg tttccatttt ataccttttt tataccaatc acttacctat
     9901 tcaacgttgt attatgatag tgtcgaaaaa ttaggaaaca ggatcgacaa aataaaatgt
     9961 aagggaggaa atctccctca tcgtttaatt aagcttcgat aggcagcaac ggaaatatta
    10021 agaataagga tgtgaatttc aactccttct aaattgttct tattatctat catccgttgc
    10081 tgtctattgt ttttttaatt attcacacga taactaaagg taagtgaaga gaaatgattc
    10141 cattaataat ttcaattttt gcgctttgtc ttaatgtata tatgattgga tttaaaaatg
    10201 ggcaaaataa aaaatagtag cagccaagaa taattttata gtgtcactgt ggcggaaagg
    10261 gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa ccatgcaagg ttcgattcct
    10321 tgccagtgac tttggcaacc gaggcatcgg cggtttaaaa atataggggt gcgcaatttc
    10381 gtacgcgttt tgtgcatcgt gtaggttgct attacataac tggttaggtt agattgagat
    10441 ttgggattcg gtacaaatga atcgtcaaat gtctcaagca caggatcgga aacgtccctg
    10501 cctgtgcatt acatattaga tcactctttg agtggtcttt ttctttacat aaaggaggct
    10561 acataatgag aaactactgg tatgtatcgc taactaatga atatcctcga accattgatg
    10621 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
    10681 tggggagaga agcagaacca tgtgagattg atgcgtgcaa gctcgtttat tgcggtcatg
    10741 gtttctatga tgaaccaaac attcaaaata atattaacaa gaatttgagg gattagaatg
    10801 caagaaatgg cttaaaaatc tccaatcagg acaaatcaag ttagacaaag tttccgattt
    10861 aaagatattg attgaagctg atttaatgct aaaagatatt gaaaattaga aaacaaaact
    10921 caacctaaac agaatgtgag gtggtgtgta ttgaatggca agacaacgtg atccaagacg
    10981 tgatgaagcc aaaagaattt ggttagaatc caacggagaa aagcagttaa aggaaattgc
    11041 atctgaatta aatgtttcag attctcaggt tagaaaatgg aaatcgcaag acaaatggag
    11101 cgctgaattg attagtaacg ttaccaatgg caaaagtaac gttactaatc aaggtggcgc
    11161 tcctattggt aatcaaaatg ctaaaggtaa caaaggaaat agccgagctt ctccgccagt
    11221 gggtaataaa aacgctttga aaacaggtga gtatgaaaca atattttttg acacgttaac
    11281 cgataatgag aaggacatct attctagttt gaatgatgat ccttcttttg ctttgtccga
    11341 agaaatacga cttcttaaaa taagacaact tcgaatgatg aaaagaatcc aacaagctga
    11401 agctggacta aatgatgaag aagtcgaacg attgcagcag ctaagaaaga ttaaaactcc
    11461 gattgaaaaa aatggtaaaa agctagaaat caagcgtgag gttatgcaag atgtgcagat
    11521 tagcagaaaa aaacatcgca aaattgatga tattctttca attgaagatt cattgactcg
    11581 gattagcaac cagttagcta aagccatcaa gcaaatgaat gaactttata tgaatgaata
    11641 cagaactgat ttaattaaag ctcagactga taagatccaa gctgagacaa atgaaattgg
    11701 cggaaataat tcaggtgaag aaatagaaga atggaaacag gcagttttaa atgccgcaaa
    11761 caaacgggcg gtgaaagaaa atgaataatg aatttattcc ttttgctgat attggttctg
    11821 ccattgatta ttactatgat aaaccagtag ctttttgcca agatattttg catttgaatc
    11881 ctgatgaatg gcaagaaaat gttttaaatg atttagctga attttcaaag gtttctgttc
    11941 gttctggtca aggagttgga aaaacagcat tagaagcagg agcaatactt tggttcttaa
    12001 cgtgtcgacc ctacgctaaa gtaatagcaa cagctccgac aatgaagcaa ctttacgatg
    12061 tactttgggc agaggtagct aaatggttaa atgatagctt gatcaaaaac ttactgaagt
    12121 ggacaaagac caaaatttat atggttggtg attcagagcg ttggtttgct acggctagaa
    12181 cagcgactaa accagaaagt atgcaaggtt ttcacgagga ccatatgttg attgtggtag
    12241 atgaagcttc tggtgtgtct gatccaatta tggaagctat tcttggtacg ctatcaggtt
    12301 ttgataataa gctgttgatg tgtggaaacc ccaataatat tgaaggtgtc ttttatgatt
    12361 cccataattc agaccgtgat aaatacagag ttcataaagt atcaagctat gatagtaaac
    12421 gtacaaacaa agacaatata gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg
    12481 ctcgtgtccg tatttttgga gaatttccga aaggtgcgtt ggattcattt atcagtcttg
    12541 aaacagttga attggctaca gaaaaacaaa ttagtgattc tttagtcaat aaaacaaagg
    12601 ttgctcatat tggtgtcgac gtagctcgat atggtgatga ttctacgatt ctctttccta
    12661 gaattgctac cagggcattg gagtatgaga agtattcaaa acgtagcacc atggaaacaa
    12721 caggatatgt catcaacatg gccaagaatc taatgagtca atatccgagt attgataaag
    12781 tgatgattaa agtcgatgac actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac
    12841 ttatagaaga caaacattat ccttttgagg tgtttggagt gaataacggt tcaacatcag
    12901 aagacgattt ttacgataat ttaggtactc aactatgggg aaacatcaag gaaatgttag
    12961 aagaaaatat gacagcaaat cttaacggag aacagcctgt tattgaattg ccttctgata
    13021 gttcgttaat caaagaatta agtactcgca aattcaaaat gacaagtaga agtcgtatac
    13081 gtttagaaag taaagatgat atgaaaaagc gaaatattgg tagtcccgat attgctgacg
    13141 cactggcttt agcgttttat gagccaccaa gtcactatca atttattcaa ttttaggagg
    13201 tgagcttttg attagtacag tattatcttt agagagatat aagaagctac gtgttaaata
    13261 tgcaacacaa attgaagatg gaatgtttga tccgaatggt tttatagaag atatgaaacc
    13321 attttttgct gatcgcgaaa gaaaatatct agcttatact agcgaaaaga atgaaataga
    13381 taatagacca aagcctaaca ctgatattgt aaaagttaat aataaacttc atgctggtat
    13441 gtattcaatt gtcgtagacc aagcagtcaa tcattttact ggtataccta tcaaatggga
    13501 ttacgatgta tcggaacaaa aaagaacact cattcaaaga ttaaaagata aattcttaaa
    13561 aaatgatata gaacttccga cagttccaga agcttttaac aaattaacaa gcaatcttga
    13621 ttccatgaga ttcgcaatgc ttgattctga aactgcaact tttcaaggag cttgtggagt
    13681 agctttccga ttgctagagc ctgtggaaga agatgatggt tggaagttaa gagcaagtaa
    13741 tattgaacct tggagagcag aaagatatgg aaatgccgga atctatatca aagaaaagta
    13801 tgactcttac cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa ataaaattct
    13861 gacatatgct tgctatggtg atttgaattt tgttacaagc ggaacgttca aaaaaattga
    13921 tgagaccgat aaccctttgg ggacgattac cttggcagaa tttaaaaata atacgaatcg
    13981 ttattgtgat tttgaagtag ctgaggaaat tggagatgca attgatcgtg ctttgtctga
    14041 tcaacaaaac gaaatcgaac aatttaagct tgcgtacatg cttgttactg gcacaacaat
    14101 gagcaaaggc actgctaaag aaatgatgaa tcagctaggg attatcaact taaaagcccc
    14161 tactgcaaaa gctgaatatg tcacaaaaaa tttagcaaaa gatttcaatg aatatcatat
    14221 ggatctattg aaaaaacagt tttacaccat ttgtaaagca atcgatttta acgatgaggt
    14281 gtttaaatca aacagttctg gagaagctcg caagtggcaa atcattagcc tagaagcgaa
    14341 aacaaatact aaagagcaat attttagaga aggattgaaa gaatgcgcag aaacaattgc
    14401 tgcttttctt aagtttcatg acaaagtaga aattgagcct gaaaaaatta tttttacttt
    14461 ctctagatct ttgccaactg atttaagtta tttagctgaa gctttaccta aacttgcacc
    14521 atacgtatcc aaacgaacta ttcagagcca aattccattt gtaacagatg ttgattatga
    14581 aaatgaaatg atggaattgg aaagtggaag tgcttatcca gatagtgaat acaattttgg
    14641 cggaggtggc aatagtgacg atagaaacga agtattggac caaacgtcga gaactggagg
    14701 atcaagcaag gctcaaacaa gaaaatcaga cacttaaaaa attaactagt gtatttcctg
    14761 aagcactgaa agagatacaa gcaaaactat tatcacaagc tgacttacac aatatcactt
    14821 atccagaaat gatggagttt tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg
    14881 aaaaaaatta taagtcatta aaaatgtatg atgcaaaata caaagagttt atcgatgaat
    14941 tttttccacc atttgactat gcaaaagtca atcgcttatt acaaatacga tcagatgtat
    15001 ttaaaattct tgcagaatat gcgatggatg cagatgtgaa tcaatatttt tctgatcgct
    15061 tagaggaaat tcttcaaaga acatattctt ctaatgctaa tgtttttgtt caacttttaa
    15121 acgttgatat accaaattat ttaccagaaa atgaaattaa acattatttg aactatccat
    15181 ggtcaggaaa gacattttca agaagacttt ggggaaatat ttcatcgctt gaacagaaac
    15241 tctctaatgc tattgtaaaa agtgttgcta gtggagaagg cgttatacac gcattaaaca
    15301 ccatgagatt agactcagaa atttgtgaca tgtttaagtt agaagaatca aagtataaca
    15361 aagcgataga aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa
    15421 aatcatattt agaaacaggt attgaggaat acaacgtatt gactgcaaaa gatgagagag
    15481 tttgccggat ttgtggagga aaggcaagta agaatcccta taaactgaaa gatgctgtca
    15541 taggtgaaaa tcgagcacct ttccatagtc gttgtagatg tacggatgtt cctaatttac
    15601 caaaattagg aaaggatatt gatgaagaat atgaccgttt atttggcgat ttattagatg
    15661 agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat gaaagatttt
    15721 tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta caaaacagct
    15781 attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa ttatgcctac
    15841 gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga taagaatacc
    15901 gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga aacagttgat
    15961 tggtatgaaa agatgggatg tattgttgtt agaactgact acaaagaagg aaagtctagt
    16021 aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact gggcttggtt
    16081 gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg
    16141 ggcgaaagga gaatgagcat gaaacacaaa tcattaatgc caatgaattt gcaatacttt
    16201 gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt ggaatccaat
    16261 gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa acaattggaa
    16321 gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga atatgaggaa
    16381 tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc tgaatttgaa
    16441 gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga acaaattgct
    16501 ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct aaataatctt
    16561 gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag tgttttgaat
    16621 gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc tgcatttggt
    16681 ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac aaatccagaa
    16741 gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa
    16801 catctttaaa taatttagag tatttggata tttcaccagc gattaatgct atgcaagtac
    16861 caaatacacc ttttttaagc tatttacttg gtgctggaaa aacagagcca gcaaactcga
    16921 cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa aaacttgagg
    16981 gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat actgaaattt
    17041 ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat ggagtcggaa
    17101 atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa
    17161 aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga atgaatggga
    17221 ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt gcagtaacaa
    17281 gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg ggagaaaaac
    17341 tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat ggaaaatcaa
    17401 caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga aatatcgtgt
    17461 ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga acaattgccg
    17521 caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca gaagaacttg
    17581 caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt gaatacaatg
    17641 cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt agtactccct
    17701 tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga tgaagtcgtg
    17761 aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa aactcgtaga
    17821 tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa agaagcaatt
    17881 gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg atggatgaat
    17941 cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg agtgaagaac
    18001 gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac acttgtaaaa
    18061 atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat aaaataataa
    18121 aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg attagttatg
    18181 cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg atacgaagag
    18241 ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt tagaaactac
    18301 ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta atacaaatat
    18361 tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc tttcacaagg
    18421 tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt caactgacac
    18481 atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa taacaataac
    18541 tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt actatcctag
    18601 tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa tatgaatcaa
    18661 atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa aaagaaggaa
    18721 tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca ttaactcctg
    18781 taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa ctatcaagcg
    18841 gtcgaattgt tggagaagtt ataaacaatg aacattattt taaatatgta gaagaaggtc
    18901 ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt gcaacgaatc
    18961 ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa atcgttaaga
    19021 aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt aaaagaaatc
    19081 tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga tttttatttc
    19141 gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca aaatgtttct
    19201 ttcttaattg atattgcatt gattgataat aaccctaata gaaatttaat taatgaatta
    19261 gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat attatttcca
    19321 aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac attagaattt
    19381 ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa aaccggaatt
    19441 atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa ttttccggta
    19501 tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc taattatgaa
    19561 acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt tggtggtgca
    19621 gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat tactggaggg
    19681 aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga tggtgcgttt
    19741 gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta taaaggacag
    19801 tttgctctag attcagatga aacatcaacg attgaaggaa gtaaagtaag ttttactcat
    19861 ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta ttcagttgat
    19921 gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa agtagcaatt
    19981 ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat caaacaacga
    20041 ttaaattaac gaaaaaagac gaagaaggta aatatgagca agtacaattt aaatctgctg
    20101 aatttttacc aggaacagtt gtagaagatg ctgcaggagt tatggaagaa atgcaaacag
    20161 cgactgataa gcaatcagtt aaaaaagctt taagtcgtgc ttattcattt attgcagata
    20221 ctttatttga gggacaattt actggtgaag attattgcaa aggaattgat gctcgtgaga
    20281 ttgcctcatt gacaggaaaa ttattgaagt ctgttactgc aggttttgat gaaacttata
    20341 cagagacgaa aaaaaagtaa gtgaggcgct caagtcacct tcatttaagt attcgattac
    20401 ttaccgagaa ttagatataa agacgcaatt actcgaagca ggttggacgt taccagaaat
    20461 tgaacgtact gacttggatg agttaatgcg tctttttgct tttagagatg cggtaaaaga
    20521 gcatgaagat gttgagtact atgataattt cactcaattt taggaggtga tactttgaat
    20581 aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc cggtatgaat
    20641 aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag tagaaatagt
    20701 ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc tagcggagta
    20761 actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg agcggttgat
    20821 cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt tagtaattca
    20881 gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca aggacttcct
    20941 actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac aggtgattta
    21001 actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt cggtggttca
    21061 actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa cggaaaagta
    21121 gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct ttctgctatt
    21181 gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttatctca aggtaagatt
    21241 tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg cggaggactt
    21301 aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc tatacaaaat
    21361 gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa taaagcatta
    21421 gtagattcag atttaggtgg atttaaggga attattgata aagtgggcag ttcaatggaa
    21481 tcgttcttaa aagtaattgc tgcaaatatt cctacagcag tatcattttt aagtaattta
    21541 tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca tgatgccctt tcttgttcct
    21601 gcagctaccg cttttggagc gttcatgttt caacttaaag gtataccagc aataataaaa
    21661 agcttcaata attttaagaa tgccataatt ggcgttggaa attcattaaa gattatgggg
    21721 gcaatagctg ctgctaatcc atttgtttta attattggtg cagtcgttgg tgcaatagct
    21781 gttttcggtt actttatggc aaccaatgaa gagcttagaa acaaggttat atctatttgg
    21841 aatgatgtga aagattccgt aattggtgta ttaaaaaata taaaggactg gggaattgat
    21901 acttggaatt ctgctaaaga aatggcatca aatgcagttg agggtgtcaa agatgcttgg
    21961 tcaggaacaa aggaatggtt ttctaacacc tggaaaggta ttaaggatgg ggccacaggt
    22021 ttatttgata aaacagtaga gacttcaaga aatgcagtcg atagtgtaaa aaatgcatgg
    22081 tctaatatga agaaatggtt ttctgatact tggcaaagta taaaagattc agccagagaa
    22141 aaatggaatg gaatcaaagg ttctattatg tcagttgctg gtccattgat tacaggtata
    22201 aagaatgcat ttttacatgt tactttttat ctagagacct tatggaataa tttagttgag
    22261 attggtaaaa acgtctttga gattttaaaa aatgttatac ttgcgccggt tttgtttatt
    22321 acctccatga tttctggtgg atgggaagaa acaaaaaata atatgattgc tgtttggaat
    22381 aatattaaag aaagtgccat aaatatttgg gaatctataa agaatatatt tgtaagttat
    22441 tttacaaata tttattttgc tgcactcaat atttggacag ggttcaagct tacattgata
    22501 aatatttgga atgaagtggt aaatcaagct aaatcgattt ggttaaacat aaagtatttt
    22561 tttattaatc tttggattga tataaaatat tttgcaattc agaaatggat tgaattgaag
    22621 ttttctatta ttcaaacttg gatcgattta aaatataatg ccattactac ttggaataat
    22681 attaaacagt ttttcaaaga tacttggaaa aatattaaag atacggcata caatacgtgg
    22741 atttctataa aaaataccat gattaataca tggaataaca ttaaggactc tttctggaat
    22801 attgttactg gaattgttaa ttctgctgaa aatgcatgga caaatctaaa aaatggtgtt
    22861 tcaaatgcta ttaatcgggt aaaagaaatc tttaattcgt taagggaagt caatttattt
    22921 gaaattggca agaacatcat tgatggactt ataaatggtg taaaagaaaa gtggaatgct
    22981 ttgaaagata ctatcaaagg tattgcaggt aatatcaaaa attggattaa aggagcttta
    23041 gatattcatt ctccatcaag atggatgcgt gatatggttg gtaaaaatat tgttcaaggt
    23101 attatcgttg gtattgataa agagcaaagt aagttagatc aaaccatgac aaatttagta
    23161 aaaacaccgt ctgttcaacc tgtagtaaat ggaataagtc acctaccaat tacacaaaca
    23221 aagcaaaata tatctgctag tgaaacaaaa gaaattcatt tacatttaaa tgtttatgga
    23281 gatttacctg attcaatggt tcggaagatt accacaaaaa ttaaaacaga attgacaaga
    23341 caaatgaaac gagatgctgg tgcagtagga ggaacattat atgcaactta agagaggaca
    23401 attttttatt aatcaacatt attcttctga atttaatgtg tatattcaaa acaggcctgc
    23461 ttctgtttca gctagtcgag taattgaatt gagagagcga gaaggaaatg attctatcat
    23521 tattgataaa ggctattaca aaaatgtgac tagaaagatt gagtgttact ataaagcacc
    23581 ttcaattgat atggtgcagg agtgggaaga tcgaatcact gaatggttag atatgtcgtc
    23641 ttatagtgat tttattcttt actatgatga acaatatatt tatcaagctg tagtaataga
    23701 aggacctgaa tttaaaggaa caagaaaaac aggaaatatt gtcccttttg aatttacagt
    23761 gagtattcga ccatttaagg aaaattacaa tggtagattt actattcgac aaacagaaac
    23821 ttttgaaatt tataacccag agaagtattc ttcaaaaccg cttattaaat tgagtggttc
    23881 tggagatgct tctttttata ttaataagga taaatatgat ttgaaatcgt tggacagaga
    23941 attatttata gattctaaat tagaagaggc ttatcgaaaa ttagatggta atttagaaca
    24001 tcaggatcag gtcaccttat ttttagattt tccattttta tatcctggaa aaaatgaaat
    24061 taaatggacg aaaaatattc attcatttga aataatgcct aggtggtgga gaaaagtatg
    24121 aaaccaagaa tatatagtcc tactgaaaca gatttctcaa cgaatggctt aggaatttta
    24181 aaggattgta caagatgtgg aatatatgaa gtagctaatg gaaaatatga attagagttg
    24241 gattatcctt taggaactag atttgatgaa tattttgaaa atgactatca aataaaagca
    24301 aagccaaacg atcaagaaga gtatcatatc ttttttattg atgataaaga tatagatact
    24361 tttttaaata cagtaactat ttatgctcag agtcgtacaa atcgacttgg aagacgggta
    24421 gtcactcttg tggaagtgga ctctaaaact ggtcaagaag cgatatcaat tattgaaact
    24481 aaaatggata aaaaatctga catacgactt tattctgata ttacggctgt ttctagcaca
    24541 atctttgaag cgagaaatgt tttaaattgt attgctggtg aacaaggatc attgcttcaa
    24601 tattggggcg gagaaattaa acgtgaacca tttaagcttt ctttgttaaa gcgaagaggt
    24661 cgcaataatg ttggaacgat tcggtatgga aaagatatgt ctggtttgaa ggtcaaatta
    24721 gattggacag gtataaaaac aagaattatt ccatatgctg atcctcaaag tgaagtaggt
    24781 acgactaatc gaatttatgg ttcgccagta gatagtgcct atattaataa ctatcctgat
    24841 gtctatacag aacatgttca gtttacagaa gaacaaggag taaaagacat taagagctta
    24901 aataaaatag ctaaaaatta ctttaaaacg atcaatcctg gctgtgataa gcctaaagtt
    24961 tctattacgg ttgaatttga taagttgact gatagtgaag aagcgaaaga atttgcgaag
    25021 attagaaatt atggtctgtt tgatacattt aaaatatatc ataaaaaata taatatttat
    25081 cttgaatcaa aggttagtgg actacaatac gattctttat ctgaaaaaac tttgaaatta
    25141 gaagcaggag acattcaagt tgctttttat cagcaacaag ctgtaactat tcaggataaa
    25201 ttaaaagatt atgcaacgaa taattatatg agtgatttta atgattatgt ttcttcaatg
    25261 attacaggtc aaggcagtgc aggaggatat gtagttttat ggccaaaaga aaaaccttcc
    25321 aacatcttta ttatggataa tccagattta gaaaaggcaa gagaagtact aagaatgaat
    25381 aaaaatggga ttgctttttc taagaaagga tggcgggggc cttttaattc agcttggaca
    25441 ttagatagta tatttaatgc caattttata caaactggat taatcaaagc agatatcttt
    25501 caaaattctt ttaacaagac tggagatgta ttgaaattag ttaatggact actccaaatt
    25561 tggaacaaca aaaagaaaat aatggagtta actaaaaaag gaatggaatt ttggaattct
    25621 aagggttcaa ttggaacgat tggaacaact gattctgttg gtaatccttt tcctggggct
    25681 tctactccaa cccctattga agataattct ttagttattc gtacaaatgg agacggcaaa
    25741 tatattttga tttctcctaa agtaggaaaa ggattagttt tattaggaaa tggtaaagca
    25801 atttattttg gagacttaga tgtacaagga aaacttacag ttaaaggaaa agaaataatt
    25861 cctggacaag gtggtggtcc atcgggaggt ggagaatcta ctggtgggta tcctagtgaa
    25921 ttaaaaacag atgcagaaaa gagagcttgg agaat
//
LOCUS       DANKBF010000037        25909 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000037 DANKBF010000000
VERSION     DANKBF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(164..388)
                     /locus_tag="PWY89_001663"
     CDS             complement(164..388)
                     /locus_tag="PWY89_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL2066706.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="PWY89_001664"
     CDS             complement(586..2166)
                     /locus_tag="PWY89_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2066707.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="PWY89_001665"
     CDS             complement(2567..3943)
                     /locus_tag="PWY89_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2066708.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="PWY89_001666"
     CDS             complement(4071..5189)
                     /locus_tag="PWY89_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2066709.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="PWY89_001667"
     CDS             5331..5765
                     /locus_tag="PWY89_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2066710.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="PWY89_001668"
     CDS             complement(5843..6376)
                     /locus_tag="PWY89_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL2066711.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="PWY89_001669"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="PWY89_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2066712.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="PWY89_001670"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="PWY89_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2066713.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="PWY89_001671"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="PWY89_001671"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2066714.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="PWY89_001672"
     CDS             complement(10101..10679)
                     /locus_tag="PWY89_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL2066715.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="PWY89_001673"
     CDS             11001..11471
                     /locus_tag="PWY89_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL2066716.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="PWY89_001674"
     CDS             complement(11579..12805)
                     /locus_tag="PWY89_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2066717.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="PWY89_001675"
     CDS             complement(13559..13759)
                     /locus_tag="PWY89_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2066718.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="PWY89_001676"
     CDS             complement(13893..14501)
                     /locus_tag="PWY89_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL2066719.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="PWY89_001677"
     CDS             complement(14586..15056)
                     /locus_tag="PWY89_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL2066720.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="PWY89_001678"
     CDS             complement(15104..16018)
                     /locus_tag="PWY89_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2066721.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="PWY89_001679"
     CDS             16164..16967
                     /locus_tag="PWY89_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL2066722.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="PWY89_001680"
     CDS             17145..18092
                     /locus_tag="PWY89_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2066723.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="PWY89_001681"
     CDS             complement(18160..18453)
                     /locus_tag="PWY89_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL2066724.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="PWY89_001682"
     CDS             complement(18481..19146)
                     /locus_tag="PWY89_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL2066725.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="PWY89_001683"
     CDS             complement(19286..19918)
                     /locus_tag="PWY89_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2066726.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /locus_tag="PWY89_001684"
     CDS             complement(19925..20992)
                     /locus_tag="PWY89_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2066727.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /locus_tag="PWY89_001685"
     CDS             complement(20989..21720)
                     /locus_tag="PWY89_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL2066728.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="PWY89_001686"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="PWY89_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2066729.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="PWY89_001687"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="PWY89_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2066730.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="PWY89_001688"
     CDS             23883..25052
                     /locus_tag="PWY89_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2066731.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(25319..>25909)
                     /locus_tag="PWY89_001689"
     CDS             complement(25319..>25909)
                     /locus_tag="PWY89_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066732.1"
                     /translation="FISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         7823 a         5180 c         4146 g         8760 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac
    25261 aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc
    25321 attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca attagcgtga
    25381 ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg attgttactt
    25441 tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt agtactgtag
    25501 cattttttga attaacagta accgttaatc ctttataccg gaatacaggt aaaactattg
    25561 ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga gaaaagctac
    25621 tataaataac ttgaccatct tctgtaactt gatggttttt caaaagcaat tgatcttttt
    25681 tatagcctat aggtacataa tcaaaattct caaattcttc aagtttattt tccatttgtt
    25741 cattagaata agttccttga aaattcattt ctttcaccat gttaactact gatgaataat
    25801 ttaaaattga aatagctaat attaataata gtataaaatt tcttctcctt gtataagaaa
    25861 atttttccaa ctgtggagaa ataaatagag atactgaaaa agatatgaa
//
LOCUS       DANKBF010000038        25837 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000038 DANKBF010000000
VERSION     DANKBF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            183..2243
                     /locus_tag="PWY89_001690"
     CDS             183..2243
                     /locus_tag="PWY89_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL2066733.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2326..2895
                     /locus_tag="PWY89_001691"
     CDS             2326..2895
                     /locus_tag="PWY89_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2066734.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3070..3876)
                     /locus_tag="PWY89_001692"
     CDS             complement(3070..3876)
                     /locus_tag="PWY89_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066735.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4014..5528
                     /locus_tag="PWY89_001693"
     CDS             4014..5528
                     /locus_tag="PWY89_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL2066736.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5931..6914)
                     /locus_tag="PWY89_001694"
     CDS             complement(5931..6914)
                     /locus_tag="PWY89_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL2066737.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6919..7566)
                     /locus_tag="PWY89_001695"
     CDS             complement(6919..7566)
                     /locus_tag="PWY89_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2066738.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7651..8283)
                     /locus_tag="PWY89_001696"
     CDS             complement(7651..8283)
                     /locus_tag="PWY89_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2066739.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8299..8850)
                     /gene="coaC"
                     /locus_tag="PWY89_001697"
     CDS             complement(8299..8850)
                     /gene="coaC"
                     /locus_tag="PWY89_001697"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL2066740.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8847..9584)
                     /locus_tag="PWY89_001698"
     CDS             complement(8847..9584)
                     /locus_tag="PWY89_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL2066741.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9581..10570)
                     /locus_tag="PWY89_001699"
     CDS             complement(9581..10570)
                     /locus_tag="PWY89_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2066742.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10828..11370
                     /locus_tag="PWY89_001700"
     CDS             10828..11370
                     /locus_tag="PWY89_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2066743.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11387..12472
                     /locus_tag="PWY89_001701"
     CDS             11387..12472
                     /locus_tag="PWY89_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066744.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12484..13290
                     /locus_tag="PWY89_001702"
     CDS             12484..13290
                     /locus_tag="PWY89_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2066745.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13291..14118
                     /locus_tag="PWY89_001703"
     CDS             13291..14118
                     /locus_tag="PWY89_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2066746.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14115..15188
                     /locus_tag="PWY89_001704"
     CDS             14115..15188
                     /locus_tag="PWY89_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2066747.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15616..18312
                     /locus_tag="PWY89_001705"
     CDS             15616..18312
                     /locus_tag="PWY89_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2066748.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18389..18877
                     /locus_tag="PWY89_001706"
     CDS             18389..18877
                     /locus_tag="PWY89_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2066749.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18889..19383
                     /locus_tag="PWY89_001707"
     CDS             18889..19383
                     /locus_tag="PWY89_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2066750.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19404..20213
                     /locus_tag="PWY89_001708"
     CDS             19404..20213
                     /locus_tag="PWY89_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2066751.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20210..21031
                     /locus_tag="PWY89_001709"
     CDS             20210..21031
                     /locus_tag="PWY89_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2066752.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21082..23181
                     /locus_tag="PWY89_001710"
     CDS             21082..23181
                     /locus_tag="PWY89_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL2066753.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23178..24296
                     /locus_tag="PWY89_001711"
     CDS             23178..24296
                     /locus_tag="PWY89_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2066754.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24335..25213
                     /locus_tag="PWY89_001712"
     CDS             24335..25213
                     /locus_tag="PWY89_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2066755.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25404..25556
                     /locus_tag="PWY89_001713"
     CDS             25404..25556
                     /locus_tag="PWY89_001713"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2066756.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8177 a         4482 c         5439 g         7739 t
ORIGIN      
        1 tatattactt aacaaaatta aaaagtacat aagttattat tccgattttc ttgaaacaat
       61 aaaaaatcaa aaaaaatact ctcctaatag tccatcttct tttgatttta tttatataat
      121 gaaatcatct tatgaaaatt actacttcat gtttagacaa gagtagggag gactaagaat
      181 aaatgtgttc aaaaagacgt tcaaaacaac taaataaaca cgcttgtcgt ttagataagg
      241 aacggtcgtg ttctgaaaaa cactacgtta agtttatgat gcgcacgatg ctgatcagtt
      301 tatctgtagg tgtctatatg catgtacccg ttcaggcaac gacacaaacc atcaaggtcg
      361 tacgtacctc tgtttctgag accactgatt cccctatcac tattactcat actttcactg
      421 atgcaaatgg aaaacagcta cttcctgcat tgacttccta taataaagtc agttatcctc
      481 caacgataaa ccactactgg ttaattccta acaaaacatt atggacatcg gaaaccggaa
      541 gcagtgtgcc gctttctatc ttttccgata tgactgatcc tacaaaaggt gatcagttaa
      601 aacagatcac tgatattttg aatcatgctt tgacagatat aaaggacggt ggaagcgcac
      661 aagtttctta tgtatatgaa aaagattatt cacagtttga aagaaagaaa atcgatgttc
      721 tggcagggac aaaagtttcg atatctgatc tggtagaaag gctgaagaac gcagatggaa
      781 cacaaggaga tatgtccaaa gtaatttcaa acaatgggaa tatcgtggat acgactaata
      841 caggaacatc tactatttcc ttgtcctatt ttgatccaat tgctttaaaa acaatgactg
      901 catctgctgt gatccatgtt cgtgctgatc aaacagctat caaaggaaaa gatcttcact
      961 taagaacagg agagagttac gcccctaaag atcttgttga atatgtaaca aaaagcgatg
     1021 gaacttcagg aaataaagaa gaaatcaaaa tgaacgggac agtagatact acaactccgg
     1081 gagaatatcc cgttgtatta acttatatag actcgaaaac atcaaatgtg gcagaaacaa
     1141 cagctgtagt ttatgtagaa gcatctgctg cttcatcgac tgtcatcact cattttatag
     1201 atagtcaaac aaataaagaa cttcaagcag aaacacatat gagtagtgta aatcgtattc
     1261 ctgatttgcg cctacctgct gaattttctg atcgagtgcc aaatatagat ttatcgactt
     1321 atgaacaaaa tgggaaagtc acttctattt ctgattggat gcaagcaaac ggattagaca
     1381 gaaaaagtca ctggtcggag atccttgaag tacttcactc agaactgatg aataatagga
     1441 agcaatcagg aggggaaatt acctttactt atgtttatat accagacaat cgttcattag
     1501 aaggtcatga tcttattgtt catccaaacg aaaaaataaa caaagaagag ttgatccaat
     1561 cagcaacaaa ttccttaggt aaacccgtac cgttaaagga tgtatcggtt acaatcaatg
     1621 aagtaccatt ggaggatggt tatttgacca aacaaacagg tgattttacg attatgtata
     1681 gttatcatga cccgtattca ctccaagaac tccatgctca gtcactatta catgttttac
     1741 cattaaacaa acagatagat caaaaagaac aaaaaagaca agaagaaagc ttgtcagaaa
     1801 aaaaacaaca aatggctcat gcagtttctc caacagggaa agtaaaacaa acaagaagcc
     1861 tagagttagc aagaatatct agaaaaatct cagaatctaa taaaacgata aggcaagaag
     1921 ctcatttggc cccacaagtt tctggattgg aaaatgaaat tttagagcct aaaataaaac
     1981 aacaatcaga aaaagaattt gttgctaaac agtcggaaga tcagaaggaa tcagatgacc
     2041 agaaggaaac gaagaaaaaa cgcttgtcaa ctattgattc caccgatgag catctgcagc
     2101 cgcaagtacc acttcctagt ccgggaacac ccgcacctgg tggaggtatt ccttcttctg
     2161 ctttaggaga tttcatgcga ggtatggcag gaacatgggt atacgctaat cgaaatgaat
     2221 ttgacgcaga tgactggttc tgatttatat ctctatctgt tatagtcaaa taagtagctg
     2281 agtcatatgt tatttggcat aatggagacg aggagggata gaaaaatgtt agataaaacg
     2341 ttaccttacg cagaaatctg gatgacaaga ccacttcaac aaaatgttcc aatcacgcct
     2401 ttagcggaag gttttgaatt tcgattttat caaaaagaag atgaatctgc ttgggcggag
     2461 attgagactt ccgttgggga atttcaaacg acagatgaag cacttgagta ttttcaacaa
     2521 aactttgctc cttatcctga agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa
     2581 ggagaaaaag tagcaacctg taccgcttgg cgaaaaaaat caagagagca agtgtatccg
     2641 gtttttcatt ggttagcagt gaaaccagat taccaaggaa aagggctcgc aaaagcattg
     2701 actgccagag tcttgcaaga atttcctacc ctccatacgg acggaccaat ctatcttcac
     2761 acacagacat ggagccatca ggcgattgct ctttataaaa aaatgggttt tacttttatt
     2821 gctgaaaatc tagacggcac acctaatcca gattatgaaa aagttatgca ggttttaagt
     2881 aaattagata aataacgaaa tggtgaaata agtcctcagt attgggataa aaaacgaaat
     2941 agtcattcct catatcttac ttttttgagt taatactatc ggataactaa tggcatgcca
     3001 tacattcaaa aaagaagggg cagtgacaaa agtattgtca ctgccccttc tcttgttttt
     3061 tagcatgttt tatcgtttga tttttttagg aataaaattc ttgatgaacg taaatttttg
     3121 gtaatcgttt cgactataga ggtaaccaaa tacactgaaa acaactgctc caataaaatt
     3181 aacaaataaa tccttcatag tatccatgat tcccacgtct aagtaaccat tttggatgat
     3241 tgttttatct gttttaccat ctgcattttg actttcaatg attgtccgag tgatatctgg
     3301 aacatgatag acagtgtttc cgtctttact taactccaca ctattgattt gctggatgat
     3361 cgtatctttt tgcatatcga attgcagtaa tttatctgct gaaaattcca caaactccca
     3421 cataacacca actgtcatcg aaaaacagaa ggaaacaact gctagtaata aaggagtcaa
     3481 gttcatggtt tttgtatttt tattgagcaa ataaacaagg gaaaagccga cacccccagc
     3541 taaaaatccg ctgaatgcat gtagaaaagt atcccacatt gaaaaatagc cataaaaatc
     3601 acttaactct cctaaaactg ttgaactaaa aataaaagat aacaaaatga tttctagcat
     3661 atttggaatc tgtatcttta atatcttttc cagaaaataa gggatttcaa ataaaattaa
     3721 tgtcagtaat aaaagcattg tatttcccca gtgttggttc cagacctgat ccactagcat
     3781 aataacgacg atgatacgta ataaaatata gattccgttt gtgattctac ctgcagtttt
     3841 tgtactgttc gtaaaaaagg ttttcaaatg attcataaaa taactccttc atgtatatcc
     3901 ttaattatac taagaagcgt gtgtttacca aagctatatg acttaaagaa aaacagagtt
     3961 gatattgtga aaaaaatcac ttaatatagt ataaggagaa aagggggata caaatgaaaa
     4021 aattagttgc tggtttatta acagccgttg cacttcttgc tatgagcgca tgcggcgcag
     4081 atcagaaaaa tacagcgcag acaagttcta gtacgaagga atctgccgaa gtatcatcag
     4141 gcgcttctga acaagtatat acagatccaa aagaaatgaa agaatcttat gatgtcgtga
     4201 ttattggagc tggtggtgca gggatggcag cagctatcga agcaaaagat gctgggttga
     4261 atccagtaat tttggaaaag atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg
     4321 gtatgaatgc ttcagaaaca aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg
     4381 ccttctatga agaaacgcta aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat
     4441 actttgtcga tcactctgct gatgcggtcg attggctaga tcaaaacggt atcaaactgg
     4501 acaacttaac gatcactgga gggatgagtg aaaagaggac acatcgtccg tctgatggct
     4561 cggctattgg tggatactta gtggaggggt tattgaataa cgtccatgag cgggaaatcc
     4621 ctatttttgt caacgcagat gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag
     4681 tgactgtaca tgttcaagga gaagaaccaa aagaggtcac cggaaaagca atcgtagtta
     4741 ccacaggcgg ttttggtgcc agcaaagaat ttattgaaaa atatcgtccc gatttagctg
     4801 attatattac gacaaatcaa gaaggatcaa ctggagatgg tatcaccatg attgaaaaat
     4861 taggtggaca aaccgttgat atggataaaa tacaaattca tccaactgtg cagcaggatg
     4921 agggggtctt gattggtgag gttgttcgcg gagaaggagc aatcttggtc gatcaagagg
     4981 ggaaaagatt tgtcaatgaa atggatacaa gggataaagt atctgcagca attacagcat
     5041 tgcctgaaaa atcagcttat ttgatttttg accaaggcgt gcgtgatcgt gccacagcta
     5101 ttgaatttta tgaccaaaaa ggatatgtca caactggaaa gacaatggaa gaattagcag
     5161 aaaaaatcga tttaccaaaa gaaacactag caaaaacagt tgatggatgg aatacggatg
     5221 ttgcggagaa aaaagatgct caattcaacc gtgccacagc catggaacat gacttatcgt
     5281 ctccgaatta ttatgcgatc aaaatcgcac caggaatcca ttacacgatg ggcggtgtga
     5341 aaatcaatac gaacactgaa gtattagata aagaaaacca gccaattagg gggttatatg
     5401 ctgctggtga agtcacaggt ggcttacatg gaaacaaccg gatcggtgga aattcagttg
     5461 cggagatcat tatttttggg cgacaagcgg gcatacaagc agctcgattt gcttcggaaa
     5521 atgaatgaaa tagaacgtaa ataaaaagag gttgtgaaat cagcgttttg agctaatacc
     5581 gagggtcagc tgattgaaca ctgtttagtg aagaggttgt gaaaaagctg ccctgcatca
     5641 agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggt tggacttatt
     5701 accgcagatg caagcttttg aacaccgttt attcggaaaa agagcgtggg acaaaagtaa
     5761 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gaaattcctt
     5821 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
     5881 tcttggagtt aaacgcttct gtcccagcct cttttttgat cagcatgatt ttattcagtt
     5941 atcttcaaaa tgatttttcc gatgtttttg tttgcttcca tatattcatg cgcttgtttg
     6001 actgcgctga attggaatgt acggtcaatg attggccgaa tcttgccatt ttctagaaaa
     6061 ggcccagctt tttgcataaa agtttgtgcg agctctgctt tatactgatc acttctaggt
     6121 gtcaataatg taccaagcag ttggacacgt ttcccaatca atacaccaag gtctatctca
     6181 ggcacgatgg tcccacctag cataccaatc aagacccaac gtccatcaac ggctatagaa
     6241 gctaaattct tttcccagta agaagcgccg atgaaatcca aaataacatc gactccccga
     6301 ccttcagtga tttttcgtac ctcttcttga aagtcttgtt ttttatagtt gatcaattca
     6361 tctgcaccaa gttctttaca aaaggcgagt ttttcttctg atcctgcagt aacgatgatt
     6421 ttcgcgtgag aaagctgtct agctaattgg atcgctgctg ttcccacacc acttgctcct
     6481 gcatggatca agacagtttc gccttcttgt aattttccaa gccagtaaag tgtttgataa
     6541 gcagtcaaaa aaacttctga gattccagca gcagaaacat agtctagcgt atcagggaga
     6601 aggattgcac gatcggcagg catcacagca tattcggcat aaccacctag attgactaat
     6661 ccgtatactt ttgtcccagg agtaaaagtg gagatatcac ttttattctc aactacctct
     6721 ccagctactt cgactcccaa aatagggtag ggaggtttca aagagactga ttctcttttc
     6781 ataatatctg ttcgattgac agctgctgta tggactttga tcagtaattc gccttgtttt
     6841 ggtttaggaa caggcacttc aatttgttct aatacatcta cagaaccagg atgcttgatt
     6901 gaaatggcac gcatggatct attcctcctt tgcccattct tggaatgctg tattcaactc
     6961 tttgctacct tcagaaagta ctagagtcgt acgagaaata tccgtttctt ccactagagc
     7021 ggcaagtaca gcagcaactg tagagcgagt gatcgttttt gatgccttct cattaggctt
     7081 agttaattga atggaagtaa cttcttcatc gttagtcaat gtgactggtt gaagaatgac
     7141 aaaatctaag ttagttcgtt tcagccattc atctgcataa tgtttagtaa tataataatc
     7201 tgtcatggac tcaggccatt ttgctggttc atctgcataa acagcactga ccatcagata
     7261 gcgagagaca tgcgcatttt cagctgcgat cactgttttg attgcgccat ccaagtctac
     7321 ttgcagaaga ttctttcctt gagagccggc agcaaaaatc aatacatcga tgcctttgaa
     7381 tgcttcggtc attttttcca cggaccaagt taaatcaaaa gagacatacg aaacattctc
     7441 tttttttact aatgtttggg aagcaacatc tctcacccct gcaaaaattt catgggaact
     7501 tggagctaaa tcttttatca ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat
     7561 tttcatttgg atcatccttt cgacgtcaaa tctttccatt caatagtaac accaaaagag
     7621 aaaaaaatca gagatcggct acgatgacta tcagttagta agagaagggt gcttgaagta
     7681 tcttaacaag acattgcata ctgctgcagt cgcaagacct gcgacgattc cttcaggtat
     7741 accacttgta aaaataaccg ctagaatagc tgaataaaca gcatttaaat ttgttccgat
     7801 gactttggca taatcttgct ggaataaaaa gtagatcata ttcatcacta aaatcgtatt
     7861 ggtcaatgaa ccggaaagtc cagctaagaa taaagaaacg gaatctgctt tttgtttcat
     7921 tagtttgcgc agacctttat aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat
     7981 aaacgcgatc agcaaggctt tccagcttcc atgttctgtt ccgatgactg ggataaacgg
     8041 agaaaagaca aaagacaaag gtgtttggat aactgtgcta cggatcatgc tgatcagtcc
     8101 aaatgtaccg ccaagaaaag cacctagtct tggtcctaaa acgatcgaag caatgattac
     8161 aggaatatgc atggttgttg cattgattgg accgataggg ataaaaccta aaggcgtaac
     8221 agctaataaa atcaaaatag ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt
     8281 cataagtact ccttttttct aagcatttct ttcattaaga gtcagcatga cctgttcgat
     8341 gatcgtctga atatcagcta aggcaccttt tccaaaatct ccacaagcta aaagagattc
     8401 tcttggttca atttcctgat agccgacaga tctcaatgtt tccagattac gctgagtgat
     8461 cgggttttga tacatattcg tgttcatggc aggggcaatc aattttggtg ttcccactgg
     8521 aagcgccatt gcgactgttg aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat
     8581 aatgtttgct gtagcaggcg cgatgagaaa caagtcagct tgttttgcta gttcgatatg
     8641 attgattacc tgcggatttg attcttgcat gacgtctgta tggactgcgc gcttcgacag
     8701 agattgcaag gttaatggcg tgataaaatg tgtgctgctt ttagacataa tcacatctac
     8761 ctgggcacct aatttgacga attgacttgt aagatcagcg cttttgtaag cagaaatact
     8821 tccagtaacg cctagtaaga tctttttcat tttgattcct ccagtctaat tttctcagta
     8881 atcaatgcag caatctcaga tttactttga gcttcttcca ctgttccatc tttagataag
     8941 agatagccgt ggtgtgtctc gccatggatt tccgtcaaat catttgcgaa aataaaatca
     9001 gttttgttat ttatcagact attttttgcg actgccagaa gagattcctt tggtacatct
     9061 actaatagtt tgaaaccaat gatttttgct tttggctgcc attcacgtag ataagaaatg
     9121 atttttggtg ttttcttcaa aacgagcaca agatgctcgg tgttagaaga gatctttgaa
     9181 acagtggtat ctgtctgttc attttccgca aaccaattgt ccagttcttc ttgcgttgaa
     9241 gaagtgggta ggttttttgc cagctgttct tctgaaaaac taaaagcagg agtaaaatca
     9301 ctgactgcca tactgtggat aatcgcatcg tagtgttctt ttttcgttag ttcttctaat
     9361 tgtaaaaata agtccagtgc agattcgatc gtataaatcg ttatattgga gtgtcttttc
     9421 ggtttcagtg cttgtttcgt agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa
     9481 taatcagcta atgcttgccc taatcgtcct gtcgaatgat ttgtaatagc acgtacttga
     9541 tcgatttttt cagtcgttcc tccagctgtg attagaatct tcatttatgg tcacctacat
     9601 ttactagtgt tcttccttgg tgtgttccag caagtagttt agatgctgtt tcaggcaatt
     9661 gatccagagt aatctcttgg acgaccagtt catctgcaat ctgcaaatcc gccatctgtt
     9721 gccataaaga aagacgttga tctattggta cattgacaga atcaattcca atcaactgga
     9781 tgtttcgcag aataaaaggc aagacagtcg cgtttagttt gattccccca gcattgccac
     9841 ataaagctac tgcaccgtta taagagatca atggaagaag actgcttagc tgctcgccac
     9901 ctactgtgtc gatgacatag tcgatttgct gttttcctaa agcttttgtt gtctctggca
     9961 gaaattcatc aggtcgtacg acttgcaaag cacctagact ttttagccat gtgacagact
    10021 cttcttttct ggaaaaggta atgattgact ggaatcctag tttatgtaac agggctatcg
    10081 ctgtactgcc tacaccgcca gaagcaccag taacgacaac ttttttatcg gaggtcatac
    10141 cttgacggat taaagcgttc acacacaaca tagcagtaaa ccctgctgta ccaaggatca
    10201 tcgcttgacg cagcgtcata ttttttggta gaggaactaa ccagtctcct ggtacttttt
    10261 gatattggct ataaccgcct ggatgggtga cgccaagccc aaaaccagta gcaagtacaa
    10321 gatcttcttt tgaaaaacgt ggattgttac tttggacgac agttccggat aaatcaatcc
    10381 ctggagtcat cggatatgtt cgtatcacct gaccagactc gctcattgcc aatgcatctt
    10441 tataattgac atcagaatag gcaattttta ccaagacttc attttcagcc aactcagtaa
    10501 atggcagatc actgattgct gtatggaaaa caggttcctt tattacctga aaagatcgaa
    10561 aagcgttcat tcgtgagaca ctccttttaa aattttcttt tatcatacca taaagcctac
    10621 tcttttattc ttgttcttcc gtaaatttag acaaaaatta ggtgaaaaat aaagaatcaa
    10681 acaactagaa tttattccaa aagataaaga aatattcagc aaaaaaaatt ttacaaaggg
    10741 ttgccttttt caataaattt cgtatactaa tacgagtggt agtaaacagg aagtttacta
    10801 ttagaaaact tcgattcgga ggaaattatg gaaattggtg agaagttaag aaatttgcgc
    10861 atccaaaaga atttgacaca agaagaacta ggcgaacgca cagatttgtc aaaagggtat
    10921 atttcccagt tggaacgaga tttaagttca ccatcaatgg aaacattctt ttctattctt
    10981 gaagtcttag gtgtgactcc agaagaattt tttcatcaag aagcagcgaa tctgcaagtg
    11041 gtttattcaa aagaagatca tacggtttat ttcgatgaag aaaacgggta tgaattggaa
    11101 tggctcgtgg ctgattcgaa tgaaaaagaa atggagcctg tcttgctgac ttttgaaggt
    11161 gcaggagaat ataagacgtt tgaaccttca ttatctgaaa cgtttatttt tgtattagaa
    11221 ggggaattag agttaagctt aggagagacg gtctatgtgg caaaagcagg acagtcgatc
    11281 tattatcaag cgacaaaaca acatcaatta aggaatcatc tcaaaaaacg aacaaaagta
    11341 ttgattgttg caacgcaatc atatttgtaa tatagggggt aaaaacgtgg ggaaaccaat
    11401 catttcattt gatcaagtag tgaaacgcta tgatgatgaa acagtattaa aaaaagtaag
    11461 ttttgagatc gaacaaggaa aattttatac actgcttggt ccttccggct gtggtaaaac
    11521 aacgatccta cgtattatag ctggattcac cgaagctaca gaaggggaca tctattttga
    11581 agggaaacgg ttgaatgatt tgccagctaa taagcgacaa gtaaatacag tcttccaaga
    11641 ttatgcactt tttcctcata tgaatgtttt tgataacgtt gcttttggat tgaagatcaa
    11701 gaaaatgtcc aaatcagata tcgaaaagaa ggtaaaagat gctttgcgta tggtccagct
    11761 tcctggatac gaaaaacggg agattagtga aatgtctggt ggtcaaagac agcgtgtagc
    11821 gattgcacga gcaattgtaa atgaaccgaa agtattgcta ttagatgaac cgttatcggc
    11881 gcttgatttg aaactacgta cagatatgca gtatgaactg cgggatctgc aacaacgatt
    11941 aggaatcacg tttatttttg ttactcatga ccaagaagaa gcgttggcaa tgagtgatga
    12001 gatttttgtc atgaataaag ggaaaatcgt ccaaagtggt acacccgtag atatttatga
    12061 tgagccaatc aatcatttcg ttgctgattt tgttggggag agcaatatcg tggacggtgt
    12121 aatgatcgaa gataatctag ttgagtttgt tggtaagcaa tttgaatgtg tcgatggcgg
    12181 gatgcgtcta aatgaaccag ttgaagttgt tttacgccca gaggatttga cgatcacgac
    12241 accagataaa ggaaaactag tcgtaacggt cgatactcag ctgtttcgtg gtgtccatta
    12301 tgaaatcatc tgttatgatg aacagcaaaa tgaatggatg gtccattcaa cgaaaaaagc
    12361 taaagaagga agcaaagtag gattggcctt tgagccagaa gatatccatg tcatgcgttt
    12421 caacgagtct gaagaagaat tcgatgcacg tcttgacagc tacgaagaat aggggggaca
    12481 agaatgaaaa caatgcgcag gatctattcc gtcccttata ttttgtggct cggtttattc
    12541 gtgatcgcgc ctgtattaat gatcatttat caatcttttt ttgatatgaa tgggcaattc
    12601 acgctgaata attatctatc ttattttacg tcggggacat cgctttctat gacgatcaat
    12661 tctgtatggt atgcattttt gatcacgttt ttcacgctga tcatcagtta cccgactgct
    12721 tattttttaa caaaactgaa gcacaagcaa ttatggctga tgctgattat tttgcctaca
    12781 tgggtgaatc tgctgcttaa agcttacgca tttattggga ttttcagtgt gcacggcagc
    12841 gtgaatcatt tctttgattt tatcgggatc gggccgaaac agatattatt cacagacttc
    12901 agttttctgt tcgtggcaac gtatatcgag attccattta tgattatgcc aattttcaat
    12961 gcattggaag agatgaatcc ttctttgatc agtgctagta gagatttagg cgcgaatagc
    13021 tttgagacct tccgacgagt gatttttccg ttatcattaa atggtgtgaa aagtggtgtc
    13081 caagcagtat ttattccttc tctttcttta ttcatgctga ctcgtctgat tggcggaaat
    13141 cgggtgatca ctttaggaac ggctatcgaa cagcatttct tggttacaca aaactggggg
    13201 atgggttcta caatcggtgt gattttaatc attgctatgt tcatcgtgat gctcctgaca
    13261 ggcgagaaga aaaaaggagg tcgctcataa atgaaaaaat ttaaatggtc gtatctttat
    13321 ttagggatcg tctttttatt cttatatgca ccgatcttct atctgatttt ttattccttc
    13381 aatgaaggag gaacgatgaa cagctttaca ggatttactt gggaaaatta tgaagctgtt
    13441 tttgaagata cacgtttgat cattatcgta ttgaatacgt tcatgctggc tttcttatct
    13501 gcattgatcg ccacgatcat cgggacattt ggggctatgg gaatctatta cacgaaaaaa
    13561 cgacgtgcac ggacagcgtt gttgagcatg aataacatct tattagtctc tcctgatgtt
    13621 attatcggtg ctagtttctt gattttcttt acactactag gattcggatt aggttttcag
    13681 agtgtattgc tttcccatat tgctttcagc attccaatcg tcgtcttgat ggtcttgcca
    13741 aaattgcagg aaatgaacga ttcaatggtc gatgcggcta gagaccttgg tgctaataat
    13801 ttccaagtag tcaaaaatat cattttgcct tatctttctc caggaatcat agctggatat
    13861 tttatggcat ttacctattc tcttgatgat tttgcggtta catttttcgt cacaggaaat
    13921 ggctttacga cgctctcagt agagatctat tctcgtgcaa gacaaggaat cagtttagaa
    13981 atcaatgcgc taagtgcgtt agttttcttg ttctccatga tattggtcat tggatactac
    14041 tttattagta aagaaaaccg tccaaaacgg ctgaagaaaa acagaagagt acgcgagggg
    14101 gtggcacggc tgcgatgaaa aaattacaat ctctgttttt aggtattctc atcattattg
    14161 cggtgctgct gcttggtgtt cgtcaacttg aaaaaacaag tggcatgagt ggagcaaagg
    14221 tacttaccat ctataactgg ggagattata tcgatcctag tttgatcacg aaatttgaaa
    14281 aagaatatgg atacaaagtg aattatgaaa catttgattc taatgaagcg atgttcacaa
    14341 aaatccagca aggtgggaca aactacgaca tcgctatacc aagtgaatat atgatccaaa
    14401 aaatgatcaa agaaaaacta gtacttccat tagatcattc gaagatcaaa ggattaaata
    14461 atattgatcc acgattttta gacttggatt ttgatccaga taatcagtat tcgattccat
    14521 atttttgggg aactttaggg atcgtatata acgataaatt ctttgatgca aatcaaatta
    14581 agcattggga tgatttgtgg aagccggaat taaaagacag tttgatgctg attgacggtg
    14641 cacgagaagt tatggggctt tctttgaata gtttaggcta ttctttgaat agtaagaata
    14701 tggcacagct gacagaagca gcaaataaat tgaatcattt aacaccaaat gtcaaagcaa
    14761 tcgtggcaga tgagatcaaa atgtatatga tcaatgaaga agcctccgtg gcagtgacat
    14821 tttctggcga agctgccgat atgatgtggg aaaatgaaca tttacattat gtgatcccac
    14881 cagaaggatc caatctttgg tttgataata tcgttatgcc aaaaaccgtg aaaaataaag
    14941 aaggtgccta tgatttcatc aactttatgc tagaaccgga aaatgcggca caaaatgcag
    15001 agtatattgg ttattctaca ccaaatgaag aagcaaaagc gttattgcca aaagacattt
    15061 cgtcggacga acaattctat ccatctgatg acacgatctc ccatcttgaa gtctacaaag
    15121 atctaggatc gaaataccta ggcatctata atgacctatt cttagaattc aagatgtata
    15181 ggagataatg gcaaaactca gtaagaatgg ggacttatca acgttctaaa gagctaaaaa
    15241 tcgtcctttt tctatctgag ttttccaatc ttctaccaaa agcggatgaa acgccgcttt
    15301 ttttgtgtta ttttattttt tctaccacgt ttttctacca gagaaaagtg gacaaaacca
    15361 ttgtaaaata gggcccgaga cgggctagta aatctaagat tgaaaaagtt acagaaggat
    15421 aaagtaattg gaaactatgc aaatatactt aacccttctg taatctatta ataaaactaa
    15481 tatatgaatc cagttcaata tttgtagcta tgaaaaaaca taaaaatcat attagataaa
    15541 aactaaattg gtagataaat tattttgtca gatatctgag gcaaatattc gactatttgt
    15601 gtttcaaagt aaaaattgga atacaaatat agaatagaca tattgcagcc gatagtttat
    15661 tatgctttta gacacattaa actggaggaa aaaatgaaaa agagcgctaa agatcgagtt
    15721 tttgatgagc tgatacgact aacagggaat atgtcttctg tcactgccga gaaaatttcg
    15781 aagaatctag gtatctctcg tcagaatgcc agccattatt tgacgagatt agtggaagat
    15841 aaaaaggtcg agaaattacc gggaaagcct gttttgtgga agcctttaga tgaatatgcg
    15901 gttgtagata atacggagca aataaacgaa gcctttcatt ctgtagttgg tcatgacgga
    15961 agtctgagag aggtcattca aaaatgtata gccgctgtca aatatccgcc taacggactc
    16021 agtgtattaa taaatggagc cacgggtgtt ggaaaaagtt ttttagccac aaaaattttt
    16081 gaatacgcta tccatgagca aattattgaa aaagatgcac catttgctat cttaaactgt
    16141 gccgattatg cggacaatcc cgaactgctt tcggcaacac tgtttggata caagaagggg
    16201 gcctttacgg gggctgaaaa ggataccgat ggtttattgg caacggccaa caacggctat
    16261 ctttttctcg atgaggtcca tcgcctatcg aaagaaaatc aagaaaagct ctttctattt
    16321 attgatactg gaaactatcg tccagtcggt gaaaatgtaa attggcattc tgctaaggtt
    16381 cgttttattt tcgccacgac ggaaaaaggt gaaaattatt tattagacac ctttgatcgg
    16441 cgtattcaaa ttagtgttat gttgccaact tttgaagatc gccccattcg agaacgatta
    16501 gagctgattc aattattttt tcaaaatgaa gcggatgttt tacaaaaaga tattattgtt
    16561 tcaagagaag ccttgtcttt aatgttatct catcccttta gtgggaatat tggtaagctg
    16621 aagaatatca ttaaaatcag ttgtgcggat gtgtatagtc gaacacaaga agaaccatta
    16681 acgattggga aaaatgagat cttgattcag ttaaatatgc tggaatccga agtagaaagc
    16741 ttgccgattg ctagtttatt gataaagaaa aatcaaaaga taaaaacgga tcgaatcaaa
    16801 ccgtttgatt atgattcgat tttggaaaaa attactcagc tgataaatga aggtaatttc
    16861 aatgaaaatt tttcttcgat aaagaatgaa gtacagcatt tggtgcatca cattgataag
    16921 gattcttttt tatcaatgag tagaaaattg atgattcaga tgtttagaaa ggtctgggta
    16981 tccgttttag gaaaaacgta cggattgacg acgagtggac ctgttgcaga aattattagc
    17041 agatcctatg tcatttcccc aagctataag acagacttaa ccgggataaa tcgtacatta
    17101 gctgcaaatc tacctcggac gacttactta tgtaatcagt tcttggagca tttaccaccg
    17161 ctctcagaaa aggacgagaa ctttctaaga ctgcttattg tcgtgacttt atctgattat
    17221 gtagatgaat ccatcgagct gaagggcctg ttgcttgctc acggagatag tacggctagc
    17281 agcattcaag cagtagtgaa tcaattatgc ggcaattatg tttttgaagc gttggatatg
    17341 ccagtagaga gtaatgtgtc agagattgtc gaatcgacaa ggaatttcgt gaaacggcaa
    17401 gtgaatacag atagtctagt gctgatcgta gatatgggct ctttaaatca aatttacaca
    17461 cagatcaaga acgatttaaa aggggagtta cttcttctga ataatttgac gacttccatt
    17521 gcacttgata ttggcttgaa gatgacgatg aatgcgccat ttaaacagat tgcggaggat
    17581 gcagcagaca agtattcaat caatgttcaa tactttgaag gcttttctca aagtagcaac
    17641 attatcattt cctgtatgtc gggtttgggg atttcggaca aattgaaaga tgtttttcgt
    17701 catcatttat cggatgaatc catcgaagtt ttcacgaagg actacagaga actacgtgaa
    17761 ttaatcgacc aaaatgatga aacttatttt gacaagacaa aattggtcat cacgacttcg
    17821 gatcttccac gttccttttc aattcctaat attaatgttt atgacatctt agacggtgat
    17881 ggagcagcca atttggagca tattttgagt cctgaaatga agccggcaga atttaaccgc
    17941 ttgattcagg agctagtaaa attcttttcg ttggaaggaa ttgcggaccg tttgaacttt
    18001 ctgaacccaa cgatcgtgat gagtgaagtg gaaacagtca tttctaaata tgaaaattac
    18061 tatcatttca ctttaaatgg ccgtgtaaaa ctaaatctct atatgcatac ggcattgatg
    18121 atggaacgtt tattcttggc tcggacagaa aaatctgaag aaccgaatta tgatttgaaa
    18181 ccagatgaag atgaatttta ttatgtttct agaagtattt ttcaatcaat ggagatgaaa
    18241 tataatttta aagtcaatgc ttatgagctt tcgctgctct atgaattatt aggaccgtat
    18301 attcaaaaat aaagtgtcta aaaaagtgtc taatctgttt tttttaggtt agacactttt
    18361 tttaatgtga attaacgtga ttaaaccaat gtttttaaaa gttggcacag gaattgctta
    18421 tataaaagtg aacagaaaga aaggaattaa ccatatgaag aaaattctaa tagcgactca
    18481 cggtcactta gcttcaggct ttcttagcag tattcaatta ctgacgggga aggttcaaga
    18541 gattacggtg attaatgctt atgtggatga gtgtgatttt gaacaggagt tgaatgtatt
    18601 tgtaaaccag gtgaacgagc aggatcagat ttttgttttt accgatttat ttggcggaag
    18661 tgtcaatcag aagattacca aaaccttttt ggagaaagag attgctgtaa ctattttgtc
    18721 tggctttaat ctgccaattc tgctcgaaat agtattggca accaaggaat taacaaagga
    18781 ggatattcag gaactggtaa ataaatgcca acaggaatta aagatgacca ttcttgatga
    18841 ggtacatgta gtaaatgaag aggaagattt tttttaggag gaagaaaaat gattaaagca
    18901 gtgcgaattg atgaacgatt aattcatgga caagtagcga tgacttggac aaagagtcta
    18961 aatttaaccg ggttagtagt agcaagcgat gaggcggcta ccaacgatat tcaaaagatg
    19021 acattgaaga tggctgcacc atcgaatgtc aaagtcatta ttaaaacagt tgatgacgcg
    19081 attcatttgt taaaagatcc gcgggcgaag gacatgcgct tgatggtttt agtccctaat
    19141 gtcaaagatg cggtaaaagt tgcgaaagct ttcccaacgg aaatcgaatt aatgaacgtg
    19201 gggaatgctg ggaaaatgac accgccatcg gaagataaga aaaccttatc aaaggaagtg
    19261 atgttaacga cgtcagaaat tcaagactta aaagaattag ttgaacttta tccagatacc
    19321 gtttttcagc ctacaccggc gatggaaaaa cgttcagcta tctctatttt gagtgatttt
    19381 tagaaaaagg aaggagaaaa attatgtatg atgcatttgt agtcgctttg gccgttttta
    19441 ttggagttgc tggacatgaa atgttcggga tgtccatgct gagtcgtccg attgttgttg
    19501 ctccgttagc aggagcgttg atgggggatt tgcagacggg gttgattgtc ggggcgtcac
    19561 tagaagccat ctttatgggt gtcgtcaata tcggaatctc tagtacggcg gagccttctt
    19621 tggctgctgg gctggctaca gcgttcgcaa tcaaaatggg aggaaacgtg gatgcgatta
    19681 ttcccattgt ctttccacta gcagtattag gattacagtt aatgaatatg attttttctt
    19741 ttgtatgtgg accgatggct gctaaatttt tcaaatttgc aaaatctggt gatcaaaaag
    19801 gaatcatcaa tctgcacttc tttatgtggt ttgtccacta tgcgctttat gctcttattc
    19861 cattctttgc cgttttattt ggagcagatg ttgtcaaaag cgtcttggat aatattccag
    19921 aggtcatcat gaacggatta acagtcgctg gaaatctgct gccggcagtt gggatggcga
    19981 tgctgttacg gatgctttgg agtaaaaata ttgctgtgtg gtttttcttt ggggccgttg
    20041 ttatggcgta tctcgagtta ccattgattg caattgccgc agttggaacg attattgcaa
    20101 ttgttatagc ccaaagagat ctacaactaa aaaatatatc tgttcatgca gttcaaagcg
    20161 atgctgctgt aaatgcaacg cttgaccaag aagaggagga tttctttcaa tgaaattagc
    20221 tgatggaatt tctaaagatg agaaaaaatt ggttcggaag atgttttggc gctcgatgac
    20281 tatgtatgct tcggtgaatc cggtgacgat gggcggcggt ggtttcgcct attctatgat
    20341 gcccttcatt aatcatttct acaaaagtga agaagatcga gctgcggcgc tggaacgtca
    20401 tacacagtat ttttcgacga ccattccttg cgcgtccttt gtcatgggga ttgcggcatc
    20461 gatggagaag gaaaattctg aaaaagctgg ttttgacgtg gaaagtatca acgcgatcaa
    20521 aacgagtttg atgggaccat tggctggaat tggcgattca ttgttctggg gagtttggcg
    20581 tgtcgttacc gcgggactag cgatcaactt agccaatgcc ggaaatattt tggcaccaat
    20641 tatcttttta gttatgttca atatacctaa ttacttgatg cgatactatg gcggcgtttt
    20701 aggctattca ttaggctcta aatatattga aaagctgtat gcgaacgggt taattgaaat
    20761 tctgacaaaa gctgctagta tcttgggtct gatcatggta ggtgcaatga cgatcagtat
    20821 ggtcaaattc aataccgttt tgagcttttc aatgaagggc gaagaactaa tgaatgtcca
    20881 agaaatcctc gatagtattt ttaaaggaat tgtaccgtta ttggtaacat tgagctgttt
    20941 caaattacta gataaaaaag tcggcgtcat caaaattatt tttggaatgg tcgtcttggg
    21001 aattttattg tcattacttg gaatcgcata gtaggaagct gcagctacta ggctgcagtt
    21061 tttttagcaa ggagggaaaa gatgactatt aatgaagtaa tttattcacc caaacaccca
    21121 ttttttaatg ctgagttaaa cgacggaaat gtgactactt caaacgaata cagctactat
    21181 cggaatattc ctgatgtaaa gggattgacc ttcgatgtaa gtaatcaaaa gacattagca
    21241 aaccttgatg tttgcgggac gattcaaacg ttgacctttt atcgagaaaa tttactaact
    21301 gaggaaaaac caggtgtttg ggtcaataaa cagctctctc aaaccaattc gttatcctta
    21361 aaggttgaag taaacgggat tgtctaccat ttgtcagaat cggatcatcg tattgaagtc
    21421 gatgtgattc aagactgttt gcctagatat attcatcatt attcgacgtt taaggtcatt
    21481 gtagtatcct ttgctcccat actggctaat aagcgtttaa gtatgcttgt tcaacaggtt
    21541 tacattgtga atgaaacaca cgaaagcctt caagtttcag tgaaagatgt gccttcgtat
    21601 caagagaagt attcggatca gcaaaatgtg ttgatttctc aaaaaggaaa taaggacacg
    21661 atcgcacaac aggaagtggc aagcttcgcc attgcttttg tggacccaaa tgcttttgaa
    21721 gaagtaatga cttttcagga atctgatatc gaaaaatggc tgaaggaaac attgaactat
    21781 tttgctcagc tatttgggca gctgtcaatg ccaaacgata gagttgtcca tctctatcgt
    21841 cgtgcacttt atcaaagctt ttctagtttc ggtatgaatc gacagggaca ggtcgtcggc
    21901 agcaattggg gcagctatcc tgctactaat cgaatttgga ataaggatat gtattacgca
    21961 tcattgccat tcaccatgtt tgaaccggag ttatgtcaaa aaacggttct atggtttgat
    22021 caatatggcg ttaagtttcc tggttcaaaa tttacaggag gtatcaatca ttctctgagt
    22081 aattcgttat ccagtgcgtt actggctagt ctttattttg aacatactgg ggatacaaaa
    22141 ttctttgagc ggtgtccgca ggttctactg aatgcttcaa gaattattga ggacatatta
    22201 gctcaacgtc ggtcagggga accaatgctt tttccatctg tttggttatc agacgccttt
    22261 gcacttggaa agtatcatac cggcagcaat ctttgtcttt ggaaggcctg tattggactt
    22321 gcagaaattt ttgaagccct agaacaactg agtcttagca agcgatacaa aaacattgcc
    22381 tgtaaactaa aggaatcaat ttgtaaacaa atgactatcg atggtccttt tggtgagcaa
    22441 tttttggagg gaatcggtga tgaggagaaa acaacctatt cagtgaagca ctatcaaaaa
    22501 ccgattcttg agcaagggtt aatcttttta agtgatgtga ttgtggatgg aaaaattaat
    22561 ttattgatgc atgatggaga agaatcagac actacgttga tgccttttta tcaattttta
    22621 gataataaag atcatttata tcagaacacc atggcctttt ctgctagttc tttcaatcca
    22681 acctatagcg aggaaatcaa aggcatcact tggggattag aatccggcgc cacttttcct
    22741 ggctttatca ctgttttaat gagcgagctg cacaaccctc aagctttcgt aacccgatta
    22801 gaagaactaa tttgtttggc agatttagac ggttcgtggt ggtggtggcc gtatcgctta
    22861 gaggctcaac aaggagaagt cgtgcgagac tttggctgcg gcaaatgcgg ctgggcatcg
    22921 ggtctttttg tcagcctaat gatcagtcaa tatttcggat tgaaattcaa aaaaggagta
    22981 ttgcaaattg atcctgttga taatttaacg ttttcttgga agaatttgaa gttcggtcaa
    23041 gccttcattt cgattgaatg tacgcaatcc gagttgagta tttcaaattt gtcagaaaag
    23101 ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag aaaagggaaa aacgattcac
    23161 attcaaagga gaaaaagatg agtatactaa aaaatattac ccaatttaaa aaaatccaga
    23221 gtcgagcaat cactcccgaa aatccaacag gcgagaaagg taaagctgcg atgttgccaa
    23281 gcgagctagg accaagccgc aaaggtcgtg gcgcaattcc gctgacttct ggtgaagaaa
    23341 ctgtgattgc ggatattact ggaacgggaa taattcgtca tatgtggatg acgattcgtg
    23401 atcgaacaga atttggcagt tttgttttac gtgatttaat cttaagaatt tattgggatg
    23461 ggtcggatag ccctgctgtt gaaagtcctt taggggattt cttttgtaat gggtttggag
    23521 agcgttgcga ggtgaactcg ttgccgatag tggttaatcc tacagggggc tttaattcct
    23581 atttcgaaat gcctttccaa acatccgcta aaattacgat cgagaattta catccgaagg
    23641 atatgtcctc atttttttat acgataaatt acgctttggt ggacgaattg gaagaaaata
    23701 gcctatattt tcacgcaaat tggcatcggg aacgcattac caaattacag gaggattata
    23761 agttaattga tcgattaaag ggtcaaggct attacgtggg tacttacctt gcgcttacct
    23821 gcctagaacg ttattggtgg ggcgaaggcg agtttaaatt ctacattgat ggggatagtg
    23881 agtatccgac cgtaacctca acaggttcag aagattattt tggcggggcg tgggcctttc
    23941 atgagtacga tgagttcgga cgtccacatg cgaaaaacta ttcaacagcc tttttagggt
    24001 atccttacca atcaaataga gatgccacaa gagatcgttt tgaaacggga aaattgaatg
    24061 ccgttcatgc ctttggtgac gacgcaatgc cacgacatgg tctttatcgt tggcatcttc
    24121 aagaccccat ctgttttacc gaagatttat cggtcacttt acaacaaatc ggcaacgatg
    24181 atgttcgctt atttgagcgg caagacgatg ttgctagtgt cgcttactgg tattcaacag
    24241 aagcttcagg aaatactaaa cgtcatttca ctgatcgaga acgcttgccg cgttaatgaa
    24301 gtgtttttca catattcgaa gggaggaacc tatgatgata tatggggcaa ttgaagctgg
    24361 agggactaaa tttgtctgtg cagtcggtac tgaggaatta acgattttgg aaagagtcag
    24421 ttttcccaca acaactccag ttgaaacaat gaagcaggtc attgattttt ttaaaccttt
    24481 taaggaacaa ttggcaggca tcggggtcgg ctgctttggt ccaattgata ttcaatgcaa
    24541 atcaacgagt ttcggtcata tcacatcaac accaaaactt gcttggcaaa atttcaattt
    24601 tgtaggaacg ctaaagcaat atttcaatat tccgattgaa tggacaacag atgtcaatgc
    24661 ggcttgctat ggtgaatatg ttgctggttc tggcaaaggg ttagcaggtg ttgcatactt
    24721 cactgtggga acaggtatcg gaggtggtgc cttgttcaat gggactttcg tggaagggtt
    24781 cagtcatccg gaaatggggc atatgttgat caagcgacat ccaaaggaca atttttcagg
    24841 aaactgtccc ttccaccaag attgtttaga ggggatggca gcaggaccag cgattgaaaa
    24901 acggttgggt gtaaaaggcc agaatttatt ggctgacgat tcgttttggc agattgaagc
    24961 cttttacctt gcccaatgtg catacaatac aacactgatg ttttcgccag atagaattat
    25021 ttttggcggt ggtgtaatga aacaagaaca catgaagaaa aaagttcagg ataaatttgt
    25081 tgagcttata aatggatatg ttgaaatacc accaattgat agctacatta ttacgcccga
    25141 attaggtgat aatgccggaa taatcggcgg attagcattg gccagaaaag cggtaagaaa
    25201 taagcaacca taattaatga atgtttcatt gatactcatg tcaaaagtat ttaaccgagt
    25261 aatcaaatga tcaaatgaag ttggataaag cgaaaatgaa ttcaaataaa aaggctctaa
    25321 gtcaaatcgt gttgttttca ttaaatcact gaaaggaaat gcttctgcgt ttcctttttt
    25381 ctttttgttg tgcgctgtat accttgagga tattcactgg tggcgtacac ttaaatagta
    25441 atatattgtg ttactttatg tctgtggtaa tagttatagt tgcttaccaa tgtatgttca
    25501 tttttcgaaa tgttgacttc ataatccttg gcttaatgtt tgaatttttt tggtgataga
    25561 ttttttcagc cgtataaaat aaatcgtaat tctattatag agagttgtga ttataaaaag
    25621 atagcaagaa aaaaaaagaa agtttctata cattttaaat attttattcc tatttctatt
    25681 gctaaaaaag tagaaattct atatttacct ttaactctcg cttctcttgc aaatgtagtt
    25741 agcattttaa tcgataataa actcaggtag ttatattacc ttgaaatgat tgataatttt
    25801 gtcacttcta ttttttttgc aataaatttt ttatttt
//
LOCUS       DANKBF010000039        25701 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000039 DANKBF010000000
VERSION     DANKBF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            133..3660
                     /locus_tag="PWY89_001714"
     CDS             133..3660
                     /locus_tag="PWY89_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL2066757.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="PWY89_001715"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="PWY89_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL2066758.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="PWY89_001716"
     CDS             7448..7789
                     /locus_tag="PWY89_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2066759.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="PWY89_001717"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="PWY89_001717"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2066760.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="PWY89_001718"
     CDS             9146..11566
                     /locus_tag="PWY89_001718"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2066761.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWY89_001719"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWY89_001719"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2066762.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="PWY89_001720"
     CDS             13000..13737
                     /locus_tag="PWY89_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2066763.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="PWY89_001721"
     CDS             13751..14566
                     /locus_tag="PWY89_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2066764.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="PWY89_001722"
     CDS             14579..15226
                     /locus_tag="PWY89_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2066765.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="PWY89_001723"
     CDS             15242..15898
                     /locus_tag="PWY89_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2066766.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="PWY89_001724"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="PWY89_001724"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2066767.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="PWY89_001725"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="PWY89_001725"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2066768.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="PWY89_001726"
     CDS             18240..18758
                     /locus_tag="PWY89_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2066769.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="PWY89_001727"
     CDS             18865..19365
                     /locus_tag="PWY89_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2066770.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="PWY89_001728"
     CDS             complement(19645..20565)
                     /locus_tag="PWY89_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL2066771.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="PWY89_001729"
     CDS             20730..21743
                     /locus_tag="PWY89_001729"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2066772.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="PWY89_001730"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="PWY89_001730"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL2066773.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="PWY89_001731"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="PWY89_001731"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2066774.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="PWY89_001732"
     CDS             complement(23383..24144)
                     /locus_tag="PWY89_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2066775.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="PWY89_001733"
     CDS             24333..25427
                     /locus_tag="PWY89_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2066776.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25701)
                     /locus_tag="PWY89_001734"
     CDS             complement(25513..>25701)
                     /locus_tag="PWY89_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2066777.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8509 a         4455 c         5417 g         7320 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat t
//
LOCUS       DANKBF010000040        24297 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000040 DANKBF010000000
VERSION     DANKBF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(101..646)
                     /locus_tag="PWY89_001735"
     CDS             complement(101..646)
                     /locus_tag="PWY89_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2066778.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(658..1350)
                     /locus_tag="PWY89_001736"
     CDS             complement(658..1350)
                     /locus_tag="PWY89_001736"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2066779.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1369..1653)
                     /locus_tag="PWY89_001737"
     CDS             complement(1369..1653)
                     /locus_tag="PWY89_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066780.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1666..2229)
                     /locus_tag="PWY89_001738"
     CDS             complement(1666..2229)
                     /locus_tag="PWY89_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2066781.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2457..3119
                     /locus_tag="PWY89_001739"
     CDS             2457..3119
                     /locus_tag="PWY89_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL2066782.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3243..4439
                     /locus_tag="PWY89_001740"
     CDS             3243..4439
                     /locus_tag="PWY89_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL2066783.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4540..5916)
                     /locus_tag="PWY89_001741"
     CDS             complement(4540..5916)
                     /locus_tag="PWY89_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2066784.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5953..6819)
                     /locus_tag="PWY89_001742"
     CDS             complement(5953..6819)
                     /locus_tag="PWY89_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL2066785.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6908..7933)
                     /locus_tag="PWY89_001743"
     CDS             complement(6908..7933)
                     /locus_tag="PWY89_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2066786.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7951..8625)
                     /locus_tag="PWY89_001744"
     CDS             complement(7951..8625)
                     /locus_tag="PWY89_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2066787.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8809..9702)
                     /locus_tag="PWY89_001745"
     CDS             complement(8809..9702)
                     /locus_tag="PWY89_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2066788.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9776..11473)
                     /locus_tag="PWY89_001746"
     CDS             complement(9776..11473)
                     /locus_tag="PWY89_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2066789.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11466..12779)
                     /locus_tag="PWY89_001747"
     CDS             complement(11466..12779)
                     /locus_tag="PWY89_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2066790.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12779..16816)
                     /locus_tag="PWY89_001748"
     CDS             complement(12779..16816)
                     /locus_tag="PWY89_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2066791.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16829..17287)
                     /locus_tag="PWY89_001749"
     CDS             complement(16829..17287)
                     /locus_tag="PWY89_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2066792.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17585..18103)
                     /locus_tag="PWY89_001750"
     CDS             complement(17585..18103)
                     /locus_tag="PWY89_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2066793.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18105..18721)
                     /locus_tag="PWY89_001751"
                     /pseudo
     CDS             complement(18105..18721)
                     /locus_tag="PWY89_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(18718..19581)
                     /locus_tag="PWY89_001752"
     CDS             complement(18718..19581)
                     /locus_tag="PWY89_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2066794.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19568..20479)
                     /locus_tag="PWY89_001753"
     CDS             complement(19568..20479)
                     /locus_tag="PWY89_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2066795.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20520..21854)
                     /locus_tag="PWY89_001754"
     CDS             complement(20520..21854)
                     /locus_tag="PWY89_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2066796.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22062..22892
                     /locus_tag="PWY89_001755"
     CDS             22062..22892
                     /locus_tag="PWY89_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDL2066797.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23136..23454)
                     /locus_tag="PWY89_001756"
                     /pseudo
     CDS             complement(23136..23454)
                     /locus_tag="PWY89_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23576..23770)
                     /locus_tag="PWY89_001757"
     CDS             complement(23576..23770)
                     /locus_tag="PWY89_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066798.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         7138 a         5281 c         4207 g         7671 t
ORIGIN      
        1 ttaaaataac gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta
       61 ttattttgtg ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta
      121 gtgtggactc aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg
      181 aatctttgaa aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca
      241 cacagatatc tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac
      301 ggatgtctag atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat
      361 tttcctcttg ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac
      421 ccgttcctat cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg
      481 gatcatgcgc atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat
      541 gagcagtcag ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct
      601 ctgcaacaaa atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta
      661 tacgagatgt ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat
      721 aaattcgtca aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat
      781 caaaaatggg atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca
      841 agctaatgct tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa
      901 tgtatcacct gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt
      961 aaccatttca ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg
     1021 tcctgggttg tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat
     1081 aacgatgtca cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac
     1141 catgttcacg ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc
     1201 tataccggaa cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc
     1261 gcctgcgcgt tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc
     1321 ttccattgcg ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc
     1381 gattgctaaa aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa
     1441 agagttccat ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt
     1501 ctttttgctt tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt
     1561 ttctgctttt cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag
     1621 ttcgcttcgc gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt
     1681 caatagttcc cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt
     1741 catcgcttgt tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc
     1801 ttgtgctaaa ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc
     1861 atttgcaaaa cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata
     1921 gccagtttct tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg
     1981 atcgattttt ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt
     2041 gacaagaagc agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa
     2101 taccagttct ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc
     2161 aataattttt ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt
     2221 gttcagcata tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa
     2281 tttttctcaa aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat
     2341 agaaaatcct tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt
     2401 aatcgaataa cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga
     2461 taaaacgaaa ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca
     2521 atagtttttc ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac
     2581 tctttggttc gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac
     2641 ttgaaactca ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa
     2701 caatgaatca aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca
     2761 aacttaaagc aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag
     2821 aattggtgaa agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc
     2881 cgaatctagt tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca
     2941 aacagaccta tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa
     3001 tcgcacaaga ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat
     3061 ctgttgcgaa acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac
     3121 tcttttgttc ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact
     3181 ttttctaaag tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt
     3241 ttatggatca taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag
     3301 atttattgaa caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg
     3361 agattgatca gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg
     3421 aacgccaaga acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg
     3481 tcatcagcta cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga
     3541 atcatgtgca agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc
     3601 gtctccaaga agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg
     3661 tcttcgggaa ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg
     3721 cacaaattga caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata
     3781 atctgatgtt agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt
     3841 atatcgctgc tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga
     3901 tgaaaagagc agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc
     3961 aattcctaga tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga
     4021 tccagcaagc accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga
     4081 tccaagcatc tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga
     4141 ccttgcttcg tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg
     4201 acttattgaa gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa
     4261 atgaacgtgg aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa
     4321 caatccaaga gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag
     4381 tagagcttgg acacatggaa gaagatctga aacaaaaact gttggattta acccaataga
     4441 tcacctttcg cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg
     4501 gcgtttttct tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct
     4561 gtgtccatag ccatagccaa aataaatgat cagtccaata accattacga taccgaacac
     4621 gatccatgtc acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat
     4681 tggcaatagc ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt
     4741 tctcaacttc agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt
     4801 tagttccgct aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc
     4861 ggcataagtc gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg
     4921 aagtaagcca tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat
     4981 cgttaccgtc agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg
     5041 tcctacatac ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac
     5101 gattcctgtc aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc
     5161 aatgattcct cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac
     5221 ggcatcaaat cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa
     5281 accaaaaggc atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat
     5341 aaacaacgcg atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt
     5401 cttggcttca agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc
     5461 aatgtatgta ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc
     5521 taaacttttc aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc
     5581 tagtaaaaat tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac
     5641 aacatacaga taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa
     5701 acttagtcca gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg
     5761 accagcattt tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc
     5821 accaattcca agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg
     5881 attgagattg atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt
     5941 ggaaaacagc gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa
     6001 aaggcaacca acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa
     6061 aatcccacct aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa
     6121 accaagtgat ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca
     6181 gatcatgttt ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc
     6241 gcctaagatc attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca
     6301 gttgacgatg atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc
     6361 cttaccaaaa tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc
     6421 agtcaaatac gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt
     6481 cgtttgtgtc cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa
     6541 accagtagac aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc
     6601 attttgtcca acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt
     6661 gattacagga ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat
     6721 tcctagtgtt tgttgattgg catcgccacc aattttacca cttactaaac caatactacc
     6781 ccatgccaac atagggacta atgctattaa aatttccata accaatctcc ttttatttca
     6841 catgataaga aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa
     6901 cgattttcta acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg
     6961 tcccttttcc ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt
     7021 ttgctaaata aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag
     7081 taaatccctt atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt
     7141 taactgaaag gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat
     7201 ccggcgtata tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag
     7261 catcggtcaa aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa
     7321 aataatttgc ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga
     7381 tgaggtagtc ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt
     7441 catcgatttt tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact
     7501 cgatcgaacg aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg
     7561 agtcaatata atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt
     7621 gttcacggat cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac
     7681 cactcaaata attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc
     7741 ttctcttgct caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag
     7801 ctaaataagc aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga
     7861 tcgtcaatgc aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt
     7921 taaaaaaatt catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt
     7981 tgtttcgata tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt
     8041 gactgtcaat gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt
     8101 ttcccgactt aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt
     8161 tttgctcaaa tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt
     8221 aatgccattg tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag
     8281 taatgcattg atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc
     8341 tgcccccatg ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat
     8401 gatcggtact ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg
     8461 aagattaata tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg
     8521 attgaaatcg gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa
     8581 ttgtcgaatg atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat
     8641 ctttcttctt gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga
     8701 aaatgtcatt ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc
     8761 tcttccaagc agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt
     8821 tttctttcgt tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag
     8881 cactagcaat atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg
     8941 ttctatcttt gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg
     9001 gtgagtgttc acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct
     9061 aattgctaat gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc
     9121 gagcatccga ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc
     9181 cgtaaagagt tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc
     9241 tcctccagtt ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc
     9301 attcgtttca tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat
     9361 catatgttgt tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa
     9421 aggatctttg acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt
     9481 cttttcatgc actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt
     9541 ttgataattc tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg
     9601 atatgtaaaa actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag
     9661 actgatttgg gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt
     9721 taaaaaaact gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata
     9781 cttcagactt cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc
     9841 cgattagtaa gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca
     9901 tattcggtac tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat
     9961 agcttccata agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac
    10021 cttctggcgc catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa
    10081 agtatccatt tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc
    10141 tatctgctcc tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt
    10201 cagaacggtc ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg
    10261 catcatattt ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt
    10321 cttcataatc aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat
    10381 catccgcttc tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac
    10441 gtgcatctgc taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa
    10501 tcgtcacgac aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa
    10561 tcgtctcgcc ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg
    10621 tatctgtgat tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga
    10681 aataaggaat aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat
    10741 aagaaatcgt ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt
    10801 cctctacatt catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat
    10861 ctgtattgta tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca
    10921 tcatcactgg catcataaca accattggag acgcgattct ggaaatatga gctggcgaat
    10981 aagtttctaa gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga
    11041 cggtagagaa taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc
    11101 gatattttaa aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag
    11161 aattaagacc gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata
    11221 cttccgttac gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg
    11281 tagaaaccaa tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta
    11341 atgtaatcgt cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt
    11401 tcccgaaaat ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca
    11461 tttttttatt catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat
    11521 aaaagaattc ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac
    11581 cattttcctg attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt
    11641 tcttttacgg tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact
    11701 ttcccggatt ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct
    11761 tttgcatcca cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc
    11821 tcgtaacctt ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt
    11881 tgtcctgtcg taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc
    11941 aaaggatcat tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata
    12001 tctaaataat atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat
    12061 ggttttcctt tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt
    12121 agataaccga catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc
    12181 gggtgataaa ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc
    12241 aatccaactt ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg
    12301 tttttcttag gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc
    12361 ccagttgtta ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata
    12421 ttgtttctgt tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca
    12481 gctttttttt catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt
    12541 ggcaccgtct gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca
    12601 tcattttcat ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc
    12661 aacacgaatt ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg
    12721 actgctgcaa gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt
    12781 accttgctcc ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg
    12841 ccatgaagaa tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca
    12901 acggattttc aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa
    12961 gttcatattc taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg
    13021 aaatctcaac ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt
    13081 caggttcttc tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt
    13141 caggtgactt cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata
    13201 gggaagaatc gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac
    13261 tattttgttt ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa
    13321 ttttcaaaag ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac
    13381 catcgatgat ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt
    13441 caatgataaa cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat
    13501 acccatttgg tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt
    13561 ggtcgtaatt taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga
    13621 tcccaggcaa acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca
    13681 gcgggaagga agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc
    13741 cttcatggtc taattttcta actcggatct cccgataatc aaacgtattg taagcagtga
    13801 atgctaccca taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt
    13861 cacctgtttg cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg
    13921 tgtcattttc atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt
    13981 ctgcatagtt ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg
    14041 gatgaataac tttctcacca tccacgatta catatttccc gtcttcatct actgaataga
    14101 acgtgagtct ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg
    14161 gatatccgat ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga
    14221 ttggcatatt actgccttca attagtttat tgatattcat gtggattggc ttgaattcat
    14281 ttcgcatttc cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt
    14341 gtacaacaga aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg
    14401 gtccgccgac tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct
    14461 catcttcgtt ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat
    14521 aacgtggtgg tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat
    14581 ctcctaaaat cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg
    14641 tttcgattgg ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc
    14701 cagccaacgg ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt
    14761 cgtaacgaat ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat
    14821 cctccaggtt ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc
    14881 ccgcatcaag ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg
    14941 gagaaaattt ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt
    15001 ctggtggggc ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc
    15061 cgttttctcc tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg
    15121 gtgagacatt aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg
    15181 tagaagagat atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc
    15241 gcggtgttac gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag
    15301 catctagtcg ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac
    15361 ttggcgggtt ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg
    15421 cagatgactt ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac
    15481 caacccagtg agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat
    15541 ttacttcatc ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg
    15601 cattagaagt attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag
    15661 caatcttatc ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac
    15721 ttgactgaat cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt
    15781 tttctggatt tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa
    15841 gccctagtgt aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc
    15901 ccgtcaaacg attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt
    15961 tattgatagc tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg
    16021 ggtcagagag cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag
    16081 tttgactatt ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa
    16141 cgatatcgat tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt
    16201 aaagggttgt tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt
    16261 ctacccattt tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt
    16321 catttcccca aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg
    16381 atccattatt agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac
    16441 tatcatcgat caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat
    16501 tgctttgtgt ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcgggtagc
    16561 gatgctgttc gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag
    16621 ctgttggccc aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg
    16681 cttgaccgct gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt
    16741 gaatttcagc ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc
    16801 tttcatcaac caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg
    16861 tttcaaaaat caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt
    16921 cttgaccatc gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac
    16981 ctgttccacg aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac
    17041 gtattctacc ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt
    17101 cttctttttc aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat
    17161 cctcctttgt ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact
    17221 ggaaatatgg tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc
    17281 ctttcacatc cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa
    17341 caatgcccat ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt
    17401 tagttaaaaa acaccaatta tacaacattt attattaaaa ttaaattata aaattttata
    17461 acgttctcat ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc
    17521 tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat
    17581 taattcagat aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta
    17641 aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa
    17701 ataaaaaggc taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa
    17761 aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga
    17821 caagccgacg atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca
    17881 aaccaacaaa aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat
    17941 caggtatcaa gactaaaaca gcagcaagga gcaaccaaat aaaaccacca ttagagatat
    18001 tactgataaa tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact
    18061 tgttgataat actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta
    18121 agaacctttt cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg
    18181 aaacctaata aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac
    18241 gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta
    18301 atccaagaag cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg
    18361 cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa
    18421 tagcggaaaa tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct
    18481 tgctttccat aaagagagaa gttctcacga aactttttcc agaatagtga accgagcttt
    18541 ttctcttcat ttttttgtaa tgataaatca tatacagaac tgttcgactt gcagttccta
    18601 atgtgaaaac aggcaagacg cacaggaacc atagtagctg taaatggata agacgtggaa
    18661 tccattcaat gatttctaag aaataatcat tggctttttt accaatcgat cgattcatca
    18721 tccttttgaa cctcctgtca aatccatccc ttcaatgaaa taacgttgtg caaaaaagaa
    18781 cagcaaaatc gtcgggatca agacaagtgt tgctcctgcc atcagataat tccattgtgt
    18841 cgttgcttgg ttctgaaaaa cttgcaagcc aatctgtaac gtgtattttt cttgatcttg
    18901 gatatataac agaggaccta agaaatcatt ccaagctgcg ttgaaggaat tgatcgcaat
    18961 ggtaatcaag gcaggtttcg tcaatggaag catcaaatga ctccaaatat acaaatgatt
    19021 cgctccgtcg atttcagcag cttcgatcaa ttcattattg atcgataagt aaaattgacg
    19081 catcagaaat atattgaacg cattgccgaa gaacgatgga acgatcaggg gcaaataagt
    19141 acctacccag ccgatcttgg agaataatac atattgaggg atcatcgtta caaaccccgg
    19201 gatcatcatc gtagaaagaa ccaaagcaaa catcagcttt ttcccaggaa aatcaagctt
    19261 cgcaaatcca taagcaataa aagaatttga aaggacatta ccgatcacta ctagcacagt
    19321 gatgaataat gtatttcttg catagcgcca aaaaggaaag gcggcaatcg tatccagata
    19381 gttttcccat acgatttttt ctgggaaaaa ggtcggagga taacgaacga tttcttccat
    19441 tggtttcaat gaagtaaaca ccatccaaag caatggaatg atcaaaatga ttcctattac
    19501 tgtcatgagg acataactta gtattttttt gtttgccgat tcacactggt ctttttgaaa
    19561 taatccatca tttgccgtca cctccagaat aatagaccca cttaggtgca agcttcaaat
    19621 tgacaaacgt cagaaccaac acgatcagaa acaggatcat tgacatagcc atcgcataac
    19681 ccatatcaaa agctacgaac gccttgttcc acatatgcag attataaaaa agcaaagaat
    19741 ttgcaggtcc gcctgttcca tttttcgtca tgacataagc ttcttgaaaa atttggaaag
    19801 aaccgatcgt agaggtgatc acatcgtaaa aaataatagg agtgattaat ggcaaagtaa
    19861 tacttttgaa ctttccccaa acgcctgctc catcgatttc tgctgattca tataaagatc
    19921 taggaacatt ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa ctccatattt
    19981 tcatcaaaat caacgcaggt ttcgtccatt gtggatcaaa cagccaagct ggtccaggga
    20041 tatggaacca gcccagaaat gtgttgatca atcctgcaga tggagaaagc agctgcatcc
    20101 ataagatgta gactgctact cctgataaga ctgccggtaa atagaaagct gtacggaata
    20161 cactgattcc tttgattttt tgattgagta ctgtggctaa aaacacactg aaaatcgtcg
    20221 tcagcggtac attgaagatg acgaaataaa cggtgttgta taacgatttc caaaataaat
    20281 catcgtgaac gaacattctt ttgaaatttg ctagaccaat gaaattcatc ttggatgtca
    20341 aattataatc ggtgaaacta ccaataactg aaaaaagcat aggccctagc ataaatacca
    20401 agaacccgat aataaaaggc gagataaata aataggccca tcttgcttct tttttctttc
    20461 ggatagataa acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc aaaaatggat
    20521 tattgatttt gcttgactaa gttttctact gctttttgtg catctgccaa tccttctttt
    20581 ggtgattttg ttcctaaaag aatctggtca atctgttcat tcactaatgt cgtgtaatcc
    20641 ggagcatcta aaggaactgg tgtgatcacc gtatttttaa agttttcgtc cgccatttga
    20701 tagatcattt ttcctttatc cgttaggttg tcattattca ctaaattctc attggctgtc
    20761 ttgctcgcca tgatatcaaa acttttttcg ccgaattttt cttgtacttc tggagaagaa
    20821 agatatttga tgaattcata agaggcctca ggatcttttg ctccgtatgg tacttccaag
    20881 acgaatccgc caccccacga ccaatggcct gaaccttctt ctttttctgg gagctgcatc
    20941 acgccaaaat caaaatcatc cggtgcattt tccatcaaac tagagtaata attgatattt
    21001 tgggcacgca tggctactaa tccggaaata aatggatcag atacaccaga cccaaattcg
    21061 gcttctaatt tattgatcgt atttttccca taataatctt gccagtcttg gatccactcc
    21121 aaggcttcca ctttattttt agtatcaatc ttgacttttt catctttgtc aaaccaacta
    21181 gtcccgttat ctgcgtttag cgcccataca tcagccccta aattccataa tggataaaaa
    21241 ccgatgcgtg taaattcttt tccttctttt ttatctagtt tacgtgctgt ttcttctaat
    21301 tcatcccaag ttgtcggtag atcttgttca ctgattccag cttctttaaa tatcttttta
    21361 ttataaaaaa tgacttgtgt atctgtattg aatgggatcg cataaggatc accttcatat
    21421 tctactgtat cccaaagctg cggataaaaa tccttagaag tgtctttttc tacatatttg
    21481 gagagattag ttgcttgctg tgcttctgct ctttgggcaa cggaatttat gtcctgtaca
    21541 ataacatcgg gtgcatttcc agctgttatt gcagataaag atttagtcca gatgtcaccc
    21601 catggctgat acttataatt tacatggatc tcgttttgtg attgattgaa atcctcaatg
    21661 atttcttcga tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa atcaatattt
    21721 tttcttccat cacttgtttt ttcagaagaa tttgctgcac ttgaactggc atcgcttgca
    21781 ttacttgatc cgcatcctcc taataaaaac aatgctgaac ttgccagcaa ccctgtaaac
    21841 aataattttt tcatatttta cctccaaatt ttaatgtaac cgatgccggt tttaatataa
    21901 cgatatcatt ttgttctgaa aataacaatt tataaggtat gattctaaaa aaattattaa
    21961 tgataccaat actttatata attcgcgagc gctttctttg aattttttaa tctttcgtag
    22021 cagaatgaga atgtattaaa aatagattag gaggaatttt gatgtttaga caccagaaag
    22081 aattgcagtt tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa tatctgcagg
    22141 agctgatcgg cggccaatat ggcgagatga gtgtagctat gcagtacctg ttccaaggct
    22201 ggagctgccg aggagaagaa aaatataaag atatgctgat ggacattgca acagaagaaa
    22261 tcggccacgt ggaaatgttg gcgacaatgg ttgctcgttt attagagggt gcgcctgttg
    22321 aagatcaaga agcagcgatg caaaaagatc caacaatcgg tgccattatc tctggaatga
    22381 atccacagca cgcgatcgta gctggcttag gacctcgtcc tgctgacagc gtgggcaatc
    22441 catggagcgg tgcctatatc attgcgagtg gcaacttgtt agcagacttt agagctaatt
    22501 taaatgccga atcacaagga agacttcaag tagcacgtat ttacgaaatg atcgatgatc
    22561 gtggcgtaaa agatatgctg tctgttctcc ttgcaagaga tagctatcat caaaatctat
    22621 gggaatgtgc agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc ccaagtactt
    22681 tcccgcgtga caaagaacga aaagacattg cttatgactt ctataacttc tcagagggag
    22741 accaaagtag tgaaggttct tgggctagtg gcaaagcact ggatggcgaa ggcgaatatc
    22801 aatggttgaa agaacctaag atcgaaggcg ctgcaccaaa attagaacca gtaacaccaa
    22861 aaatgtatgg gacacctcca ttcaagaaat aaatatctac ccgtagaaac acccgttttt
    22921 tgttccacac gctttgtgag caaaactgac taaagtcatt aatcataacc acccttcaaa
    22981 agggtggttt tctctagagc tataagctca aatcaccggc cagcgcctaa agacgctggc
    23041 tttcatattt tatgttcaag ccatattgcc tttgccagtt ttgcccacct cttaaaaggt
    23101 ttatgtacta ctccttagcc cttaaaagga tcgtcttact ctcgtacact cagcttatct
    23161 aacgcactat catgtttttc ttgttcttga atgtactttt tgatggtcgc ttcattttgt
    23221 cctacttttg tcacatagta tccttccacc caaaagtgtc gatacccaaa tttatatttt
    23281 agatttgcac aatctcttga tgatctcctg aatattggct cgatattgat tataaattat
    23341 ttttcttcta tactttggtg tgaatacaat gtggtattta cacatctact ttgtacgggc
    23401 taaactattt ccctttattg ctccacccgc atagcaagtg gttttgtatt tcaatcgctc
    23461 ggcgcgctca attaggtcta aagacaataa atctaaaaac ccgctgcttc actgacagaa
    23521 acaacgggtt ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat ctattttatt
    23581 tttttgattt tttataaatg gaaaatgctt caagaagtcc aagaaataaa gccgtataaa
    23641 aacaagaacc tatgaaatct ttatttatta gatattcaac agaaatcccg ataacactag
    23701 ccaacaatat atttatcaga agtgtaaaat aaggatgctt tttatagaat agaactagtg
    23761 ttgttttcat agactctcct ttcttcatat ttccaattaa tcacaaattc acggcattac
    23821 tagaaaaatt aaaggggcgc agaagtggaa aaatactctt cgactttccc tacaaaccag
    23881 gccatcttgt gtttaataac tataccaaat ctaaggtacc tctaaatcta aattccattt
    23941 gtggcggtga cctccaattt ttaactcttc tatctcccat ccagtataca taaccttgat
    24001 acttcgacca cttatatatc gaacacacaa atacctatcc attgcaggag tactacctaa
    24061 cttagttgct acttgcatct aaataacagc tgaaaagata ggacttactc ttagtaaagt
    24121 atctgaacta ttaattttta aactatcatt tttatcctca actacatgca catctgttgc
    24181 aacttcactt tctgtctcat ccagataaat gattaaggaa tcgggaattg caatatcttc
    24241 agctgacgct ggaatagttg agctaaatta gtcatcatat ctttattata aaataac
//
LOCUS       DANKBF010000041        23647 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000041 DANKBF010000000
VERSION     DANKBF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="PWY89_001758"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="PWY89_001758"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2066799.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="PWY89_001759"
     CDS             complement(1174..1869)
                     /locus_tag="PWY89_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL2066800.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="PWY89_001760"
     CDS             complement(1882..2598)
                     /locus_tag="PWY89_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2066801.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="PWY89_001761"
     CDS             2928..3536
                     /locus_tag="PWY89_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL2066802.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="PWY89_001762"
     CDS             3861..4184
                     /locus_tag="PWY89_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2066803.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="PWY89_001763"
     CDS             4366..5736
                     /locus_tag="PWY89_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2066804.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="PWY89_001764"
     CDS             complement(5866..7167)
                     /locus_tag="PWY89_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2066805.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9479)
                     /locus_tag="PWY89_001765"
     CDS             complement(7521..9479)
                     /locus_tag="PWY89_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL2066806.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="PWY89_001766"
     CDS             complement(9656..9922)
                     /locus_tag="PWY89_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2066807.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="PWY89_001767"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="PWY89_001767"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2066808.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="PWY89_001768"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="PWY89_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2066809.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /locus_tag="PWY89_001769"
     CDS             13266..13829
                     /locus_tag="PWY89_001769"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2066810.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="PWY89_001770"
     CDS             complement(14082..14603)
                     /locus_tag="PWY89_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL2066811.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="PWY89_001771"
     CDS             complement(14606..15448)
                     /locus_tag="PWY89_001771"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2066812.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="PWY89_001772"
     CDS             complement(15441..16166)
                     /locus_tag="PWY89_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL2066813.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="PWY89_001773"
     CDS             16342..16638
                     /locus_tag="PWY89_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066814.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17206..18798
                     /locus_tag="PWY89_001774"
     CDS             17206..18798
                     /locus_tag="PWY89_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2066815.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="PWY89_001775"
     CDS             18811..19551
                     /locus_tag="PWY89_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2066816.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="PWY89_001776"
     CDS             19719..21068
                     /locus_tag="PWY89_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2066817.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="PWY89_001777"
     CDS             complement(21196..21786)
                     /locus_tag="PWY89_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2066818.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="PWY89_001778"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="PWY89_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2066819.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6771 a         4762 c         4503 g         7611 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttatt gccgataggc tgtctcttga
    23341 acactgttta ttgagaggtc gtgaaaaagc cgctcagttt cgagtattaa gaaatatttt
    23401 ttgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
    23461 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
    23521 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
    23581 aacatttctt ttataatata gattttagac atatatatga tataaaaata gttttcgcaa
    23641 aaaaact
//
LOCUS       DANKBF010000042        23177 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000042 DANKBF010000000
VERSION     DANKBF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            7..624
                     /locus_tag="PWY89_001779"
     CDS             7..624
                     /locus_tag="PWY89_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066820.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="PWY89_001780"
     CDS             1644..2813
                     /locus_tag="PWY89_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2066821.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="PWY89_001781"
     CDS             3286..3840
                     /locus_tag="PWY89_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2066822.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="PWY89_001782"
     CDS             complement(4013..4486)
                     /locus_tag="PWY89_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2066823.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..4602
                     /locus_tag="PWY89_001783"
                     /pseudo
     CDS             4498..4602
                     /locus_tag="PWY89_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4711..5790
                     /locus_tag="PWY89_001784"
     CDS             4711..5790
                     /locus_tag="PWY89_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2066824.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            5976..7145
                     /locus_tag="PWY89_001785"
                     /pseudo
     CDS             5976..7145
                     /locus_tag="PWY89_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613029.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
     gene            7293..8552
                     /locus_tag="PWY89_001786"
     CDS             7293..8552
                     /locus_tag="PWY89_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2066825.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            8562..9434
                     /locus_tag="PWY89_001787"
     CDS             8562..9434
                     /locus_tag="PWY89_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2066826.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            9446..10276
                     /locus_tag="PWY89_001788"
     CDS             9446..10276
                     /locus_tag="PWY89_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2066827.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            10316..12499
                     /locus_tag="PWY89_001789"
     CDS             10316..12499
                     /locus_tag="PWY89_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2066828.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            12560..13780
                     /locus_tag="PWY89_001790"
     CDS             12560..13780
                     /locus_tag="PWY89_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL2066829.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            13777..15237
                     /gene="gtfA"
                     /locus_tag="PWY89_001791"
     CDS             13777..15237
                     /gene="gtfA"
                     /locus_tag="PWY89_001791"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL2066830.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            15314..16420
                     /locus_tag="PWY89_001792"
                     /pseudo
     CDS             15314..16420
                     /locus_tag="PWY89_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            16742..16951
                     /locus_tag="PWY89_001793"
     CDS             16742..16951
                     /locus_tag="PWY89_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2066831.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            16944..17093
                     /locus_tag="PWY89_001794"
     CDS             16944..17093
                     /locus_tag="PWY89_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL2066832.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            17247..17912
                     /locus_tag="PWY89_001795"
     CDS             17247..17912
                     /locus_tag="PWY89_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2066833.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(18196..19281)
                     /gene="xerS"
                     /locus_tag="PWY89_001796"
     CDS             complement(18196..19281)
                     /gene="xerS"
                     /locus_tag="PWY89_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2066834.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            19615..19848
                     /locus_tag="PWY89_001797"
     CDS             19615..19848
                     /locus_tag="PWY89_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2066835.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            19845..20252
                     /locus_tag="PWY89_001798"
     CDS             19845..20252
                     /locus_tag="PWY89_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2066836.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(21389..21907)
                     /locus_tag="PWY89_001799"
     CDS             complement(21389..21907)
                     /locus_tag="PWY89_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL2066837.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            23025..>23177
                     /locus_tag="PWY89_001800"
     CDS             23025..>23177
                     /locus_tag="PWY89_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2066838.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         7931 a         3647 c         4277 g         7322 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaaggcgga ttttataatt
     4621 aacttagcca tttggaaata aaaaaacgcc acgtgaattc catgttatat tgaagttacc
     4681 acaactctca atagacaagg atgaattcac atgacgcaaa accatgataa cacagtttca
     4741 cgcaagggaa aacacctaac ctactcagaa cgttgccaaa tcgccatcct gaaaaaggaa
     4801 aactattcca accgtcaggt tgcggatgtc ctgggctgcg tgccgcagac cataaacaac
     4861 gaaatcaaac gcgggaccgt tactcagctt aaccgccaga atcagaacgg gaaggtctac
     4921 gactattatc atgacgtcta cgacgccgat gccgggcagg cggcctacga gaagaaccgc
     4981 ttgaactgcg gcagacggcc cttatgggcc gacacggacg ccttcattga atgggcggac
     5041 gacatgatgc tgttggaaaa atggtccccg gacgtggtgg tgggctttgc cagaaaacac
     5101 gaattgttcg acccggccat tatccctggg accaccaccc tctatcagtg gatcgaccgt
     5161 ggcatcatga aaacgaagaa tatggacctg ctagaaaaac tgtcccgcaa accgaaatcg
     5221 tcccgtcatg gcaaccgccc gaataaacgg gtgctaggga aatccattga agagcgcccg
     5281 gaggcggttg gtacccggaa aaccttcggc cattgggaag tcgacacggt cgtaggaaac
     5341 aagaccaagt cggatgccgt gctgctgacg ttggtggaac ggcagacacg cttcgaggtc
     5401 attctgaagc tggccgggaa ggatgccgaa tcggtcaacc aagcggtccg acagctgaga
     5461 gaacgggctg gtgcgtactt cggccagctg ttcaagacgg ttacctcgga caacggctcg
     5521 gagttcgcgg gactgcacga tgccttgcag gatgtggcaa acatctactt cagccacccc
     5581 tatgcttcct gggagcgcgg gacgagcgaa aaccaacaca agctgattcg ccgtttcatc
     5641 cccaagggca gcccaatcgg caacgtgagc gaaaaccaaa tcttgcggat tcagcgttgg
     5701 atgaacgatt acccgcgaaa gattttgggc tatgcaacgc cgcatgacac gttcgtccag
     5761 gcatttaggc aggaacggtt gacggcttga cacgtgggcc cttgacaacg agcctccctc
     5821 ggcatggctt gtgtctggaa agctggccgg ggccagccag tcgggagact accatgcctc
     5881 agctcattgt caagggccca ctcgattaga aaaattagac ggttggtggt aacgagcaac
     5941 cgggtcacca tgggtggcta acttaaactt gaaatttacg gcttaaaaat attaataaaa
     6001 attcgaatga aaatcgtgct gatattgttt cttattatga tcgcaaagaa gcgttaagag
     6061 aggctgattt tgttatcaat gctattcaag tgggtggtta taaaccttca acagtgattg
     6121 attttgatat tcctaaaaaa tatgggttgc gacaaactat tggtgacacc atggggattg
     6181 gtgggatttt tagaacccta cggacactac cagtaatgtt agactttgct aaagaaatgg
     6241 aagaagtttg tccaaatgct tggttattga actatacaaa tccgatggcg atgttaacca
     6301 tgggtgtttt aaaagcgacg agtattaaga ctgttggttt atgtcatagc gttcaagtat
     6361 gcgtaccaga attgtttgag catttaggaa tagccgatca atatgactta aatgactttc
     6421 aatggaaaat tgcaggcatt aatcatatgg cattcttgtt ggaaattaca aaaaatgggg
     6481 aagatttcta tccaacaatt cgtcagttag ctgcagaaaa accaaatcca catccagatt
     6541 ctgtacgttt tgaattgttg aaacgttttg gttactatat tacagaatca agtgaacaca
     6601 atgcagaata ccatccatac tttattaaat caaaataccc agaattgatt gacgaattag
     6661 ggattccgtt ggatgaatac ttacgtcgtt gtgaacaaca aattaatgat tgggaaagca
     6721 tgcgagagga cattgtaaat aatggggagt taacacatac gagaagccgt gaatatgctt
     6781 catatatcat ggattctatt acgacaggaa ctccgtcagt gattgcagga aatgtcttaa
     6841 ataaaggctt aatcacaaac ttaccgacag atgtttgtgt ggaagtacct tgtttagtcg
     6901 ataaaaatgg cgtgcaacca acctatgtcg gtgaattgcc aacacaatta gctgctttaa
     6961 atcggacgaa tatcaatgtt caagaactga cagtagaagc cgctttaaca ctagataaat
     7021 caaaaattta tcaagcagcg tatttagatc cacatctatc atctgaattg tctttaacag
     7081 atatcaaaaa tttagtggat gatttaatcg aagcacatgg ggactattta cctgaatata
     7141 attaaaaaga agctgaaaga gtaaggaatt ttcaattcct tactcttttc ttagttaaat
     7201 ttagtaaaaa tattgacaaa acacttagta aacagttaca ttagtgttaa caaaaagaaa
     7261 acgcattcaa attgtataag gaggagcata aaatgaagtg gtctaaaaaa attattttag
     7321 cttgtatagc actaggagca acgatctctt tgactgcttg tggaaaaaat caggaagatg
     7381 caagcggtaa ggtagaaata gagtttttta atcaaaaaaa agaaatgact caaacgattc
     7441 aggagattgc taaagatttt gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg
     7501 taccgaatgc tggtgaagta attaaaacac gtatgcttgc aggcgatgta ccagatgtca
     7561 ttaatcttta tcctcaaagt atagaattga aagaatgggc gaaagctggt tatttagagg
     7621 acttaaccga agaaccgtat ttagagaata tcaaaaatgg atatgcacaa cgttttgcta
     7681 ttgaggatcg tgtatatagc ataccattaa ccgctaatgt ctatggtttt tactataaca
     7741 aaactgcttt tgaggaaatg gggattcaag caccagaaac ttgggcagaa tttgaaaaaa
     7801 tagtagctga gattaaagat caaaacaaag taccgtttgc gattgcagga gcagaaggct
     7861 ggactttaaa tggttatcac caactagcta tagcgactgc tgctggtggt gaagaggaag
     7921 cgaacaatgt ttggcgtttc tcagaagtta atggaattaa tgctgaaagc aaagaaatgc
     7981 aaaaagattt taatcgatta gatttgttaa gagaaccaaa agctttgcaa aacaactggc
     8041 aaggtgccgg ttataacgat acagtagtta cttttacaaa aggtgatgcg ttgattatgc
     8101 cgaatggttc atgggcaatg ccgatgattc attcacaaaa tccagatttt gaagtaggaa
     8161 cttttccttt tccagcagat gaagcaggtc aaagtctaac gattggcgca ggagacctag
     8221 ccttatcagt ttctgcaact tctcaacata aagaagaagc caaacgtttt gtagaatata
     8281 tgacaacacc tgaagcgatg cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg
     8341 agggcgttat cgagaatact caggattcac cattaagtgg tttaactgaa cttgctttta
     8401 ctgatcgtca tttagtttgg ttagccaaag attggaatag tgaaaatgat ttttatacct
     8461 taacaacaaa ttatttacac aatggcaatc aaaaagccat gattaacgca ttaaatgctt
     8521 tctttaatcc aatgaaagct gacggaaggt aggaaaataa aatgaaaata aaagaaattt
     8581 taaatcgtta ctgggcaatc atttttgtag caattccaat tattttgcag atgatttttt
     8641 tctactttcc aatggttcaa ggtgcttttt acagtttaac taattggact ggtctgactt
     8701 ataattttga ttttgtgggt ttgaacaatt ataagattct tttttctgat ccgaagttta
     8761 tcaaatcgat tggcttcact ttaattgtga cgctttgcat gattgctgga caaattggtt
     8821 ttggtatttt gattgcgcgg gcgttgaacg caaatattaa agggaaaaca ttctttagag
     8881 catggttctt ttttccagcg gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa
     8941 tttttaacta tggtttacca aaaattggta gtatgttagg cattgaattt ttacaaacca
     9001 gtcttttagg aacaacatgg ggagcgattt ttgcagtaat ttttgtttta ctttggcaag
     9061 gaattgccat gccagtcatc attttcttag cgggttttca aagtattcca caagaaatta
     9121 ttgaagctgc tagaatggat ggggcgaatg ataagcaaat tttttggaat attgaaatca
     9181 cctacttatt accaagtatt tcaatggtat ttattatggc attaaaaggt ggcttaacag
     9241 catttgatca aatttttgct ttaactggtg gtggaccaag cgatgccaca acatctttgg
     9301 ggttacttgt ttataattat gcttttaaaa acaatcagtt tggttatgcg aatgctattg
     9361 ctttagtctt atttatctta attgggattg tttcactggc acaattaaaa ttatcgaaaa
     9421 aatttgaaat ctaggaggtt tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata
     9481 cttgctttta ttattagggg gcttcatgat tttagttccc ttagtttcaa cgctattcgg
     9541 ttcatttaaa acaacacccg atattatgaa taatttcttt cgttttccta atccagcgac
     9601 tttggaaaat ttccgaagat tgttagcgga tggaattggt cattactttt ggaattcggc
     9661 aatcatcaca atcattgcag tagttgctgt gacagcaatt attcctatgg ctgcttattc
     9721 actagcaaga aaaatgactc gtgggaaagc atacggattg atgtatttat ttttaatttt
     9781 aggaattttt gttcctttcc aagtaattat gattccgatt accgttatga tgagtaaaat
     9841 tggtttagca aatatgtggg gattaatctt actctatctc acttatgccg taccacagac
     9901 actattttta tatgttggtt ttattaaact aaatgtgcct atcagtctgg atgaggctgc
     9961 tgaaattgat ggggctagtc actttacaac ttatttcaaa attgttttcc caatgttaaa
    10021 accaatgcat gcgacgacta ttattcttaa tgctctatgg ttttggaatg atttcatgct
    10081 accattatta attttaaatc gtgattctaa tatgtggaca ctaccattgt ttcaatataa
    10141 ttatacggga caatatttta acgactatgg tccaagtttt gcttcctata cagtaggaat
    10201 tgtcacgatt acactagttt atcttgcttt ccaaagaagt attatagctg gaatgagcaa
    10261 tggagcagtg aaataaccaa taaattattc tcattataaa ggagttcata aacttatgat
    10321 tatatttgac aatcacacac aaacatttca tttacaaaat aaaaaaatta gctatctatt
    10381 aggaatcgaa gaaaataatt atttaacgca tctttatttt ggaaaaaaga ttacgcacta
    10441 ttctggtggg tatcgctacc cccgaacaga tcgttccttc tcacccaaca atgcaaatgc
    10501 gcaagatcgt ttattatctt tagacacatt accattagaa tttccaggat atggacatgg
    10561 tgattttaga gaaccggcgc ataatattaa attaggaaac ggtagtcgtg taaatgattt
    10621 tcgttatgat agctacgaaa ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt
    10681 atatacggaa gatgatgatg aagctgaaac gctgattatt caattgaaag acgcgattag
    10741 tggattgact ttacagttgt tctacacaat ttatcaaaat cacagtgtaa ttattcgttc
    10801 tacgcaatta gtaaataata gtgatgaaac agtggagatt aaccgcttag ctagtcaatc
    10861 aattgatttt ccaaatcgtc cactcgagtt aattcattta aatggtacct gggcaagaga
    10921 acgtcaaatg actagagaaa aaattcatac agggataaaa gtattagata gtaaacgcgg
    10981 atcaagcagc catcaacaaa atccgtttgt tgctttagta gatgccaaaa cagatgaatt
    11041 tcaaggagag gcatatggat ttaatttagt ttatagcggt aatcacgaaa cggtgattca
    11101 aaaagatcca tttgaccaaa cacgggtcat tatgggaatt aattcattta attttggttg
    11161 gcaactaaaa actggcgact catttcaaac gccagaagtc gtgatggttt atagtaatga
    11221 aggtctgaat acgttatcac agacgtatca tgaattgtat aatcaccgtt taattcgtgg
    11281 aaactatcgt ttaaaagaac gtcccacgtt aatcaacaac tgggaagcaa cgtattttga
    11341 ttttaacgaa gaaaaaatca tgaatattgt cgatgatgcg aagaacttgg gcattgaaat
    11401 gtttgtttta gatgatggtt ggtttggcgg tcgtgaaaat gacttcactt cattaggtga
    11461 ttgggttgaa acagaaggca aacttgcttg tggtttggaa aatttagcca agcaagtgca
    11521 tgataacggg atgaaatttg ggatttggtt tgaaccggaa atgatttctg aagatagtga
    11581 actatttcga gcacatccag attgggcatt gggtattcca ggtcgtgacc gctcaattag
    11641 tcgaaatcaa tatgttcttg atttttcaag acaagatgtt cgtgataata tctatcaaca
    11701 aatgacggcg atttttgatc gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa
    11761 tatgacagaa gtctattctg tcttgttgga ctcaacgatg caaggtgaag tggctcatcg
    11821 ttatatgtta gggttatatg aatttttaga aaagttaaca acgaattatc caactatttt
    11881 atttgaaagt tgttcgggtg gaggcggtcg ttttgatgca ggtttcttgt attacatgcc
    11941 acaaacttgg acgagcgaca ataccgatgc cgtggctcgt ctgaaaattc aatatggaac
    12001 cagcttagtg tatccaattt caagtatggg tgcgcatgtt tcagcagtcc caaatcatca
    12061 aacaggaaga gaaacgagtt taactattcg tggcaatgct gcgatggctg gtgtttttgg
    12121 ttatgagcta gatctaacgc aattaacttc tgaagaaaaa gcagaaatta aagaacaagt
    12181 tgccttttat aaaaagcatc gccaactttt gcaatatgga actttttatc gcttggaaag
    12241 tccgtttgaa ggcaatgata ctgcatggat gtttgtctca aaagaccaaa cagaagccat
    12301 ggtcttttat ttccgagtat tagcagaagc tgcgcatcca ttagtcacat tgaaattagc
    12361 aggattagca gaagaagcaa cttatcaaat ggaagatgta acatttgctg gcgatgaatt
    12421 gatgaatctc ggtttttata ttgactcaga actacatggt gattatgcga cacaacgctt
    12481 ttatttgaaa cagatatagt agcttgaatt tacaatataa gtgcaacacc aaaagagact
    12541 cattccaaac gaagaaaata tgttttattt acacgcattt actaaaaagc agcctgattt
    12601 gaattgggaa aatgaagaag tacgtgaaga aatctatgca atgattaact attggttaga
    12661 taaaggacta ggcggattta gaatcgatgc gatattaaac atcaaaaaaa aactagagta
    12721 tggcatcttt gaatctgatg gagaagatgg gttagccttc attgggccct ggattcttaa
    12781 tcagtcgggc attgaagtgt ggttaaaaga aatgagagag cggacattta aaccacataa
    12841 tagtatgacg gtagcagaag ccgatgttcc taatgaacgt ttagatgaat atattggtga
    12901 agatggctat tattcgatgg tttttgattt tagttatacg gatattgatg tacctgaaac
    12961 tggtgaatgg tttaaagata gccaatggac atggacggat atgcgtacca atatttttac
    13021 gaatcaatta gttacgcaag ataaaggttg gggcgcctta tatttagaaa accacgatca
    13081 accacgttca attaataaat atattccaga agaatggatc aatgattata gtaaaaaaat
    13141 gttggcgacg ctttttatgt tactaagagg tacccctttt atttatcaag gacaagaact
    13201 aggaatgacc aacatagaaa tggcatcatt agaagatttt gatgatgtag ctacgcattc
    13261 tcagtataaa cgggcattag aatatggatt ggcaccagag caagcattga aagctgtcaa
    13321 taaacgaagt cgggataatt ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag
    13381 attctcaact tctgataatg tatggttaaa agtaaatcct aattatccag aactcaatgc
    13441 agaagctcag ttaaccaatc aacagtcggt attcaattat tatcatcaat taattaaatt
    13501 aagaaaaaat agtgtataca aagacgtgct aatctacgga caatttctac cagtgaaaga
    13561 taaaaatgaa catattttgc tttatgaacg ccaattagaa gataaaatcg tattagttat
    13621 cttaaacgtt acagcaacag aacaagaata ttatgtagat gaacagtacc aacaagtatt
    13681 aatcaataat tatgaagatg ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt
    13741 tgaaagtatt gtattagcta attatggagg acaattatga aaattaaaaa cgaagcaatg
    13801 ctcatcacct atgcagatag tttaggaaaa aacatgggcg aattaaaaca agtcttagac
    13861 aaacatttac aaggtgttat tggtggtgta catctgttgc ctttttttcc atcgacaggt
    13921 gatcgaggct ttgcgccgag tgattatacg acagttgatc cagctcttgg aacatgggaa
    13981 gatgtggaag cattaggtga agattattat ttgatgtttg attttatgat taatcacatt
    14041 tctcgtgaat caaaattttt ccaagatttc aaaaaaaacc atgagcagtc accatacaaa
    14101 gaaatgttta tccgtattca tgaatttttc ccagaaaatc gaccaacaca agaagatatt
    14161 gatttaatct acaagcgtaa agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt
    14221 acgacagaag aggtttggaa tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa
    14281 gaagttgtga aagaatttat tcgagaaacc atcaaagata tggcgaacca tggctgttct
    14341 ttaattcgat tagatgcgtt tgcttatgcg attaaaaaat tagataccaa tgatttcttc
    14401 gtggaaccag atatttggga acttttagac gaagtgcgtc aagaagcagc gaaatatgac
    14461 gtcgaattat taccagaaat ccatgagcat tattccattc aaatgaaaat tgccaatcat
    14521 gattattata tctatgactt cgccttgcca atggtcactc tgtattcact ttatagtgga
    14581 aaatcagaac gtttagccaa ttggttgaaa atgagtccga tgaagcagtt tacaacattg
    14641 gatacgcatg atggaattgg tgttgtggac gctcgtgatt tattaacaga tgaagaattg
    14701 gattacacct ctgaggaatt gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt
    14761 gccaattaca ataatttaga tatttatcaa attaacagca cctattatag tgcattaggc
    14821 gataatgacc gtagctatct gttagcacgt gcgattcaag tatttgcgcc agggattcca
    14881 caaatttatt acgttgggtt attagcagga aaaaatgata ttgaactgtt ggaagcaacg
    14941 aaagaagggc gtaatattaa tcgtcattac tatgatttag ccgaaattga agaagaagtg
    15001 caacggccag tgattcaaca attatttgca cttttaaaat ttagaaatga atctgcggcg
    15061 tttgatttgg atggaacgat tgaagtagca acgccatcag aatcagaaat agtgattaca
    15121 cgtaaaaata aaaacggtag tgatagtgca acattaacgg ctaacttgga tacgaaagaa
    15181 tttatcattg agtctaaaga tataaaaatt ttcactagtg ttgcattaaa attttaacct
    15241 attccataga acaaaaaatc ctttatacta gcacttggat gacttatcca agaccaataa
    15301 aaaggactca tacatggata agtataaaac aaatacagca tttaataaat ggttttctgc
    15361 cataaaatta ggaaaacttt ctgtcaactc ccaaaaatgt atttttgatt acaattcgta
    15421 tcgtaaaaaa ctgtcttttg aagctgtgct caagctcttt ctctacgcag ttaatgaaga
    15481 aaaagaaagt ttgcgtgatt tgagtacagc tcttatcgac cgatcgctcc aactagaaac
    15541 cgatgtgtca tcgattagtc atacccaatt gtctcgagca tttaatgcac ttgaacctaa
    15601 agtgttagaa gaaatttttc aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac
    15661 caagcgaaat catctgtatc tgattgattc cagtacgttt tcgcttagtt taaaacgtca
    15721 taagtgggcg actttccgtc aaacaaaatc aggtgtcaag ttacacttaa actattgtta
    15781 tatggacgat actacgatgt atccaactga ttttacctta acaaatgccc atgaacatga
    15841 tctcaatcaa ctaccagtat tggtgaatca accagaggca acttacgtgt ttgatcgtgg
    15901 gtattaggac tttgaaaaga tggatcagat gcattgggat ggctatttct tcgtcactcg
    15961 gattaaaaaa aacaccaaag ttcacgtgat agatactctg gaaacatcgc cagaaacaga
    16021 aattctacga gacgagctgg ttcgtttagg atctaaaacc tatctaaccg ccaatttccg
    16081 actagtcact gttcaagata agaacggacg tgtgtttcaa tttatcacca atcgaatgga
    16141 cgtctcttca aaagaaatct ctgatatgta tcacgctcgt tggcaaatcg aattgttttt
    16201 caagcacatc aaacaacaca tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt
    16261 gcagaatcaa ttgattttaa cgatgatttc cgctctcctc acgtttctca tcaaattgga
    16321 gacgaaaacg gaaaaatcag tgttccaaat caaacgattc tttcgttatt tactgtttca
    16381 gccctttgaa tgttgtctag aaaaattaat tcccacatga gaaaaaagaa atttggctcg
    16441 ttacttttgg ttttcagttg aataacagat aaaaaatttt ctggaaaaag tcatcctctg
    16501 cgtcagcaca gttttttttg acttattttc agaggaaata gttttcagaa tattctaaaa
    16561 tgttttatgc aacactagtg atatttttta gaattagatt tctaatgtgc attttttgag
    16621 tattaaggag caagtataca aagatttata aagctgatta aatttgaata aaaatatgca
    16681 ggaacgctac tatttttagt agtgttcttt tttatatttg aataaaaaat gtggtggtta
    16741 aatggtggtt ataacaaaaa atgaattaat gaaattaggt tatgggaagt atcagtctga
    16801 gagaatcata cgacaggcaa aagcaattat ggtttcaaaa agctatacct attacgaaaa
    16861 tagaagatta ggactagtac ctgttgaatc tgtagaagaa gttttaggta tgaagataga
    16921 ggtagaggag gattttcaga atcatggtta attttaaaaa agataaaaat gggaaatatc
    16981 attttactgc tagtttggga ttcgacaaac gaacagggaa aagagtacaa aaaaacgtag
    17041 aggggttaaa acagttaaag aagaaaaaaa gcgtataccg aacttgtttc taattatgga
    17101 aaagaagcag aaacttctca aagtactatg ttgtttgagg agttttttta taaattattc
    17161 ctaccttatt atcaagggaa agtagaagaa agaacatata aaaatcggtt atctacaatc
    17221 aaaaaaactt ttcttatttc tttaaaatgg agttaagaga aatacgtcca attgacattc
    17281 aaaagtggca aaataattta ttgagcaaat ataaaaattc ttatgtaaga aacatgtatg
    17341 ggatttttca gatgtcatta gatcgagcag ttgttttggg actacttgaa aaaaatcctg
    17401 caaaagttat tggaaatgtg aagaaaagga aaactacagt tgatttttgg acaaaagaag
    17461 aatttacaaa agttatttca actttttata ttgatgattt gtaccaacat tttggtttta
    17521 tttgtatgtg gttacttttt atgaccgtta tgcgtttggg agaagccact gcattaactt
    17581 ggaaagatat tgattttgaa aataaaaact tgcgtgtgaa taaaacactc tattataaga
    17641 gagccaaaac ctatgagttt gtagaacccg aaacggcttc tagtattcgt acaatcgaac
    17701 tagacgacaa tacaattacc tttttaaaag cttggaagga gcgtcaagtt tcaataggag
    17761 gcatgtcgtt tgtactttct tataatggaa cacctactca gaaatataca atccgtcata
    17821 ttatgagaag acatgcaacg atcgttggtg ttcatataat ccgcatacac ggattaaggc
    17881 atagtcatgc ttctttgctt atatcacaat aaaaagatgc acttcgaatt aaagaaaggt
    17941 taaaacatgc tgatgtacaa acgactttag gaacttatgg tcatttgtat ctaaattcat
    18001 tgagagagat aacaaatgat cccacagatt atatccaaac taatttttct aaaatcaaac
    18061 cgacacctat ttacgataat aaaaaataaa tgataaacaa aagtaggatc acaccaaaag
    18121 aaacacacta gaaaaactat aatacaggct tttctagcgt gttgtttctt atttccgcct
    18181 atttatacat tatactcata aagcatttaa tgcatcgatc gtagccgcat tgtctatatg
    18241 ggtatataaa gttgttgtat tggtacttga atgtcccaat tgttgggcag ttaataccaa
    18301 tgaatttgtc tgttggtaga gtctagtagc taatgtatga cgcagtttgt gagggctgac
    18361 ttgaattcta aagccttttg aatattttgc taccatccgt tcgattgcaa taccggatat
    18421 acgcttggct tctccttggt aaattgctag aaataaagct ttttcgtttt ctgttacttt
    18481 atatcgttct ttccgaatag ctagataatt agctaaatat tccattgcga atgctgctat
    18541 attgacagca tctcttttat ttccttttct agttactacc acattattct tatctaaaga
    18601 taggtcatct aaattaatat ttataacctc ggagagtctc aatccagatc ctaaaatcag
    18661 tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttact
    18721 caaagtattt ttatattcat tggcaatata atctagataa ttttgtgtat ccttatctag
    18781 aaataatttt ttctcaatgg cattggcacg tgcagctaac gtagttttat ctgtgatggt
    18841 tgctacttga tccataggat tattgtctaa gtatggtttt cctagaggat tattggaact
    18901 tttcgaaaga tatttaaata gaacctttaa tgccgtgata tttctattga tagtagaagt
    18961 agatagacct aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt
    19021 ttcttttgtg cgacgtttca aatgagattt gtacaagttt aggtcatagg cagttaactg
    19081 tcttatcgag gtgatagaag tatcaggaac gatagactca ctcagaagcc attcataaaa
    19141 gagcttataa ttttttagat attctaaagt ggttcgaggt gattgatctt tatcctcacg
    19201 atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg
    19261 atatatttct gtatttttca tgtcattcat cctttcaata agtaggggag aagagtatct
    19321 ctataattgg actataccaa ttatagaatg ttttgtcaat tggtatgctc taaaatttat
    19381 taatttgttt caaaaaaagc atataaacag gtatacgttt aataatttaa aataaatggt
    19441 atacataaat agggaaggaa atagaaggga gcaatctaaa ttttttgaat tttcttaatt
    19501 aattatatag aaagaacaat ctttctatag agttagacat gagagggact atgcttgatt
    19561 taaaatcaac aagcgtatat actgtatata caaagaaaaa ggagggagaa gactatggca
    19621 attaaagaaa gaaaatttag ggaagtgggg aactcagtag tattaactat ttcaaaggag
    19681 tttcttaaag aaaatgggtt aaatcccgga gatacggttt ttttagatga agaaaaactt
    19741 cgagcggcaa ttacaaaaaa acaagcctta tctgatgaaa ttgatttagc aatatctcaa
    19801 gcattaactg aatatgatac tacttttaga gagcttgtcg ataaatgagt attcattacc
    19861 tagaagccaa agatattata aaaatgaatg taatccaaat aaaaaaatac tctcctaatg
    19921 aaccccttgg tgtaaaggat gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct
    19981 cagcttttgg tgaagaggtt tatccttcga tttatgaaaa agcagctatc ctacttatac
    20041 aattgataaa gaaacatcct tttcacaatg caaataaacg tactgctttc ttagctactt
    20101 ttgtcttttt gaaaatcaat ggacagcttt tagctattga tcaaaaagat gctgtaaatt
    20161 tagcggtata tattgctacg tatgataagg atttcgatca actaaaatat gaagtgatca
    20221 acactataaa gacacatacg cagcccattt aaggagttac tgtatgtgtc tttatagggt
    20281 tattagctgc tatagcgaaa taattgaagt aaattacaaa tagctataat tcaaatccaa
    20341 ctatatagaa aacgtaaatt ttctatatag ttggattaag ataacgacta tttattttat
    20401 catattttta ctatacccta tacttttact tggtatattt atgaataatc gcaaatttac
    20461 ccctatacta ctagtttgta agttagatta ataaaaatac taaagttatt tgttctttta
    20521 ttaaatacac ctacaactta taaattgatg gtatttagat aacacgaaag aataaaaata
    20581 acagctgata gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt tagtgcgtat
    20641 gaacttgctt ctgaaaactt atgaaatgaa agacctttta catcttgcag aaatcgttac
    20701 tgggattaaa ctaagcaata aaaactaatg ttcccctcat tctttgaact tctactattt
    20761 gatttattta ttctgataaa tgacgatttc accttcagga tcttcaaatc ccgtatagaa
    20821 tttatttaag atctcttcag tatcagtaat aggataggcg ataggccaca taccctttga
    20881 tggttagaat aaactgctca aaatctgtat cgtatgttgg ctttagcata ttaacaaagg
    20941 ctatctccaa attataaacc ataagcgttg aatcgttctt aacaatttca attcttccta
    21001 acccaaagtc agaagatata attaccggac ttgccttacc cttcatgtgg gttgttgaga
    21061 ttccatattt aaagggaatt gtcggtacta tcggcggtct gaaaaacatc ccaaaaataa
    21121 aaaaatcttt cccctgctag tctcctaagt ccctttgtag cggatatggc accataatct
    21181 cattttatcg aagtgagtca tggtttatct gttggccaac caaaattgat acaatctaac
    21241 gatgcaccct ctacggcatt ctaggggagt gcacttgcat tcacccaata aaaaccggca
    21301 gttagcaaag aacttatgta gtcgtatgtt cttactgtta acatacaaga ggtctccttt
    21361 ccacaattgc ttagcagtaa acgggaattt acaaagctaa tctcttccat tttcttatct
    21421 ttgttctaaa agtcccaaat ggagcaactg tatttacatg aataaattta tatacttccc
    21481 atacagccgt tttggttgcc tcgtctgccc attttctttt gtgtgcagtg aatagatctt
    21541 catcactcat tgaatcaatc atcatgttaa tatcatcaat attacgattg agaatttcct
    21601 ctaattcttt caatggtaga tgtgcatagg tatttgtaaa ccacttatat aactctccta
    21661 actgattcca tttaaattga tcagaaggcg ttttaacaga taatccattt ctttcatcat
    21721 tttcccattt tagaattaaa ttcgtccaac caacttgata tgctaaattt tcagcaggtg
    21781 ttctatccac ttcctcaact cttttatcct tcaattcttc aggaatagtt tcaaattcta
    21841 aaatatattt agtataagtc ctatttattt ctttttttaa ttcttcttta tttttatatg
    21901 ttctcattat attacctcca taaattccta attgtctcct ttaattcatc tataatttca
    21961 tccacgatat tttaatccgt tctacaatgc atatctgata tgcattgact gtctaactca
    22021 tatgtgaatg atttataaaa tatcccaacg tctcatctaa agaaatgttg atcgtcagca
    22081 aggagctaaa aaatacgaat atatttcttt tgtagtaaaa tcggactggt ttgcatatca
    22141 ttttcttcgt aaatatcaaa atcatgttaa ctactatcaa ttactaataa aaactaccac
    22201 tttaagcgtt aagaactgtt aagtttagtt aagtgaggat agttcacaaa atccattgtt
    22261 aaacttcacg aaagtggtaa aactcaaaat gctttatata ggattacagc atcgctcttt
    22321 cttcgattgc gcgttgggta aaaaatatta tgaagtaaaa attgacgatg acacgttcct
    22381 tacggctcat cagatgaaac agctacaaaa acgaaatgat caactcgaag aaaatttaat
    22441 tctaaaaaag cgattgccat attcacgcct cgctcgacga actattaaag gtcaagattt
    22501 acctgaagat catccttttt aggaaattga agctttttat gttgcacaat gcatttataa
    22561 tactacaatg atgttttcac cagatattct tctagggtgc ggtgtaatga aacagataca
    22621 cttgcaaaag aaaattcacc gcgaatttac taaaattatg aataattatg ttaatcttcc
    22681 gaaatttttt tccaaaatta agggatcaag ctggtgttat agattgttta aggttggcaa
    22741 aaaaataact tctttttaaa caattataga aaaccgatac tctgttaagt tggttgacac
    22801 ggtgtcgatt tttatgactt tcttattctg aaacttcact aacggacagt acctaaaaag
    22861 tctatagtat ggacgtttac gagtactatt ttatcaaaaa tgctgctcat ttcaacttat
    22921 atgttttagt gaactacccc cacttagctt ggataggcta caattaacta gacagaaaaa
    22981 ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct aagagaacac
    23041 gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta gctggaaagc
    23101 cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac aaatgggtaa
    23161 agcaatctaa aacgagt
//
LOCUS       DANKBF010000043        23032 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000043 DANKBF010000000
VERSION     DANKBF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..2563)
                     /locus_tag="PWY89_001801"
     CDS             complement(<1..2563)
                     /locus_tag="PWY89_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066839.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
     gene            complement(2785..3669)
                     /locus_tag="PWY89_001802"
     CDS             complement(2785..3669)
                     /locus_tag="PWY89_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2066840.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3764..4201)
                     /locus_tag="PWY89_001803"
     CDS             complement(3764..4201)
                     /locus_tag="PWY89_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066841.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4418..5329)
                     /locus_tag="PWY89_001804"
     CDS             complement(4418..5329)
                     /locus_tag="PWY89_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2066842.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5706..6344)
                     /locus_tag="PWY89_001805"
     CDS             complement(5706..6344)
                     /locus_tag="PWY89_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066843.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6348..7118)
                     /locus_tag="PWY89_001806"
     CDS             complement(6348..7118)
                     /locus_tag="PWY89_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2066844.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(7536..7952)
                     /locus_tag="PWY89_001807"
     CDS             complement(7536..7952)
                     /locus_tag="PWY89_001807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066845.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(8003..8384)
                     /locus_tag="PWY89_001808"
                     /pseudo
     CDS             complement(8003..8384)
                     /locus_tag="PWY89_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8510..9175
                     /locus_tag="PWY89_001809"
     CDS             8510..9175
                     /locus_tag="PWY89_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066846.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="PWY89_001810"
     CDS             complement(9572..11605)
                     /gene="brxL"
                     /locus_tag="PWY89_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HDL2066847.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11723..14245)
                     /gene="pglZ"
                     /locus_tag="PWY89_001811"
     CDS             complement(11723..14245)
                     /gene="pglZ"
                     /locus_tag="PWY89_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HDL2066848.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14361..17993)
                     /gene="pglX"
                     /locus_tag="PWY89_001812"
     CDS             complement(14361..17993)
                     /gene="pglX"
                     /locus_tag="PWY89_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HDL2066849.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18057..21728)
                     /gene="brxC"
                     /locus_tag="PWY89_001813"
     CDS             complement(18057..21728)
                     /gene="brxC"
                     /locus_tag="PWY89_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HDL2066850.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(21753..22328)
                     /locus_tag="PWY89_001814"
     CDS             complement(21753..22328)
                     /locus_tag="PWY89_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HDL2066851.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(22328..22927)
                     /locus_tag="PWY89_001815"
     CDS             complement(22328..22927)
                     /locus_tag="PWY89_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HDL2066852.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6993 a         4531 c         3342 g         8166 t
ORIGIN      
        1 ggatagaaag aacaaattta cctgtatcat caatagttgt ttcggacaaa acagcttgat
       61 tttttttcaa tgaaaccaaa gctcctgctt cgcctgtacc tgtgattgag cgagaagctg
      121 cattgatcaa tgtggtactt aatttagcag gctcaggagg aatcacatcg actgtcgttt
      181 tggcagagtc cagttctgtc acttcttggc cgcctcttac ttttttagca gcaactattt
      241 gcacttgatc ccccgttgtt agtctttctt cgttgggtac ttgaacctca aacaaaccgc
      301 cttgttcgcc ttctccccac ggattatatc ctgtcccttc attttcatct gtttgtcctt
      361 gtgtgagagc agtaatttct acaggtgatt tcgtcgtatc tgcataagta atacgtaaag
      421 tcacttctac ttcgcctgtc caagcatctc ttggctttgc ctcgacacct tcgggaacaa
      481 cgacatgccc atagattttt ttatctgcat tcgttggtac tcttaaggat tcaatcatcg
      541 gaagctggtt attggcactg attcgattat aatttttcat tccggcacct gtctgagcaa
      601 attcatttgt taaacctgca ctagggcttg atacaaaatt acctaagtcg gttccattga
      661 tcgataagtc tgtcatatca gaaaaataat ccggatcttt ttcaaagtta tcgaacgtta
      721 ctcctgcctt tcgccataaa gcaatttctg aatgagcgat cttcaaagaa gaatcgccat
      781 ttccttgaaa tacccagcca cctatgccat tagtctcgct tccgatccgt ttgttttgga
      841 aatcgaaata ggtaggagat tctaagtcta cattcaacac atccccactg atgatgccag
      901 tcttatcttc cccagttttc ccgaccatga ctaaataagt tttttcgcca gccataaagt
      961 tcaaatcaat atctccagtt gtatctacgg tagctccgga atctgcgcgt atatcgatat
     1021 ttgatttttc tccagtaacc gtaaaatcag ctgaccctgg actttctaga aaaccagaag
     1081 cgtcataaaa ttggatcgct tggtttcctt gattgagtct gttcacataa gatttgccat
     1141 cccctgcatt tcttaccttc aatgagcctc cattagttac tgttagtttt tgcgtaccgt
     1201 tctcaaaacg aactgcgcta gaacgaccac tttgcttatc aatatcgatg actgatccgg
     1261 ctccttctac gatcaaagaa agtcctttcc ctacaaaacg catcccattg tttccaggtt
     1321 ggtcgccatc tccagccatt ttgaccgaga tttgtccacc acttgataca tggatttgac
     1381 tagaatctcc ttgcaggcga aatccgccag tagtcgctgt attgattgtg attttcccat
     1441 cattcgacac agcgactctt gatcctgagc ctctcatttg gataattccg cgattgttgt
     1501 cgttggtaat attgatcttt gtatttaccg aaaattcact tcctgagcca ctgactttaa
     1561 atgtgacatt cccgggtagc gtatcgacag cctctccttc atcgctgacg ccatctgccc
     1621 gatcatagtc aagagagacc tttgaaccac tcaagatatg gagtcctggt gctgtttttc
     1681 cactgcccgt ctcatcacct gaatctagaa aaccatagaa ttttcctaga tatggtccat
     1741 aaagctgact accattgatt acatttagct gatagcttct acaaaaataa gctttacccg
     1801 gagcggcatt catcccttct ttcactggtt ggacatccaa tacaccggaa acaccatcta
     1861 atgtgacagt tgcacgaggg gcgtaaatca ggcctaattt agaaccatca tataagtcaa
     1921 gtgtcccggt aaattttatt tctcccgtaa aatgatatct gtttccccaa aaatcggaca
     1981 tataagcaat gataatggca tttccatcga tatttggtat caacccacct ggatgagctg
     2041 ccgttccttt aaaattctga aactcgatcg actgaggtgt ctcattgata tagatgtgat
     2101 gtgtgccgaa attatgagca agtccgccaa aatcgataac gatatctcgc ttgatctcca
     2161 attccctgtc taaagtcaaa ggctgagtta aacgaatctt agctactgat tcgtctgcga
     2221 gaagctgcgt cagttcttct gttgtggcag cttcgctgta cccatccgct gaaacaactc
     2281 taacctgtac ctcttcactg ctatcagacg tttgttcagc tacttctgag gtctcacttg
     2341 tcttattttc ttcttgcctt gtattagaag taggcgcctc actttgggat tctgtagttt
     2401 cagaagtaat tgattcttct gaaaaagtag tcttgtcaaa actttgattc tctgattgcg
     2461 ttgattcgct tgttttctct acatcggcaa aaacggatgt caccatcaag agagggaaga
     2521 caaacaaaat cgacgcaatc agctttttta catattgatt cattctccac catcctctct
     2581 gacgttttga caattttatt ttaacgtttt aatttatttg taattgtact ttcatattta
     2641 aaatatgtat ttttatggca aatttttctg ttaggtgaaa atttcttttt tgtacaaaaa
     2701 aaggctgtga caaaagtctt gtcacagtcc cttattccga acaaccgatg tcctcctgac
     2761 aactcttcgg tcacaacctc cattttaaca ataaacctgt aaatgtgagg gtaacacttt
     2821 gattgtcaca ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc
     2881 atcagctaat gacacaacta attgctcaca agtaagatag gctagatttt tcgtagattc
     2941 atctactcct tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt
     3001 taaataaacc aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa
     3061 accgcctact gagttagtta gtcctatcag caagatactg ctctcaattt cttccttctt
     3121 tccatcaact tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga
     3181 catgaaataa gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattgc
     3241 ttcagggata gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat
     3301 atccaatggc ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg
     3361 tatccctaat gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg
     3421 ccgatgttct tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat
     3481 gacaaacaca ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc
     3541 tgcttttttt gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc
     3601 taattgctca aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac
     3661 ttttttcatc actttctttc ccttctgatt gatttcttta atataacgat agcataaaga
     3721 aaaaaattag gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat
     3781 aaaataccca tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa
     3841 atgataggca ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa
     3901 atagcaagag aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca
     3961 gaggaatacc aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacgacctt
     4021 gaataataaa tgcaacaatc cccgctataa ttagtgtgcc taataccaaa acggaaaatt
     4081 gatgaatata ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa
     4141 aaacaaatcc gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca
     4201 tttatctact tctacgtgca ttttttaata tcgttttctt tatttaagat tatacagaaa
     4261 aatatctagg gtggctggat tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc
     4321 aagtaataat taaactactg ttgatttagt tctaataaaa agatttagat gaacagtttt
     4381 attgatttaa atagttcata ttttttattt ataccgctta tttactactt gctgtactag
     4441 aatctgaagt gaatgtactc aaaacatttt caaagtcttt gtctttgatt tttacattag
     4501 cgtctttcaa tacttcgcca attactttat tctggaaggt actgtcactt aattttgtgt
     4561 ctgttgcaat ttctttcaat tcttttttgt atttatccat gtcgttgcct ttgttttggt
     4621 ttttcaccat tttaaccaca taatattccg tagtataagt tgaagcattg gtagaagtga
     4681 tcacatcaga aacttgacca tctttcaact taaaggctgc ttctttgact tctgccggaa
     4741 ccgttgtagt agtggaatca aatttcactt ttccgccatc ttcttttgtc gtagtatcag
     4801 ttgacttgtc tttggctaat ttagagaaat cgtctccctt atcagcagct ttcttcacat
     4861 cttttgcatc atcttcactt gctacttgga taatttgagc ttccacttct ggatggaaag
     4921 attcccaagc cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt agaccttctt
     4981 gaaaggctag actttgtttg atactgtctt tgaatgtttg ttctgtgtaa cctgcagatt
     5041 tcaattgtga atcaaatgaa tcacccaatt gtttctttgt ttgatcatat tgggaatcga
     5101 tttctttggt tgatacgtca tctccatatt tatcttcaaa tacttgataa acgatcaact
     5161 gttgcacagc ggaatctgct ccttgcattt ttttcaagct atccattact tcttgttcgc
     5221 tgattgtacc gcctttataa ctaacggctg ttttttcacc ggcattcgta cagcctgaca
     5281 ataagccagt gcctagtacg atggcaagtg ctaaaaatct ttttttcatt tatcaattct
     5341 cctacttttc gttttttgcg aattgctaaa aacaaaggtg ttcaagatga aaatcatcaa
     5401 gaacaccttt actaatctgc tcgatacgtc ctgaatagat ataaggctac acaatccaga
     5461 aagttcgctc agcagaataa atgtcaagta gcacatcaca ccttaatata ctacttcttt
     5521 tgttaccgtt tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat tcttagaaca
     5581 aatgttatcg attttgtttt tcttcttccg ctgttgggat attcaaatca gacactttta
     5641 tgttcgattt tccagacatt tttttgttgt ttttattaga aaaagtttgt tttttcattt
     5701 gttttctaaa gcatgatctt cttgtttcct attttggtca gatatggatc atgcgtgacc
     5761 acgataacta acgtaccttg ggaagcctgt tgcgataaga tttcaacgac ttgctcctta
     5821 tttcgtttat ctaatgatcc agttggttca tcggctaaaa tgattttgct attcttcaat
     5881 aagactctcg cacaggcgat tctttgcttc tcaccaccag ataatgtgta gataggcgta
     5941 cttagcttct tgtttatatt tacttttttc aatgcctcat actttgctgt tcgcttatct
     6001 gaaacattta aattcgaaaa cttcaatcca atatctaaat taaaatcgat tgtttcatat
     6061 tcaatcaacc caaaatcttg aaaaagaaaa gagagggcat ttctttttat caagcgcttc
     6121 tctcttccag ttaaatgttt tgtttcttga ttaaataaat aaaattgccc tgaactgaat
     6181 tgatcattca atcctaatat attcaataaa gtacttttcc ctgctccgct ctctccaact
     6241 acagtcacaa aatccccagc atccgcagtg aaacgatcca aatgaagaat cgttttgttt
     6301 cgaatgattt ttttcgcacc ttttatcact atcaacggct ccaattgtta acctccattc
     6361 atcagcaata actgactttt cttttctgta gacaaaataa aaacaactga aagaataaaa
     6421 tcaatgaagc taaagaaaaa aatattaaaa agatatttag gctctctttc tataagaaaa
     6481 aggaagatta cacttgtcgt tccccatttg atgaggttca tcatgaagaa atctttatac
     6541 gtttcaaaga gcgaataacc acttgaacga aagatagcga ttttccgaca attgtagaca
     6601 aaatatatgc ataacatgac atagctcaac aagaagaaag aaaagaaagt gagtaaaaat
     6661 atctgtatca agtaatgaaa tgatctgctt aacactttga cagctatctc ttcagtctga
     6721 gtatacggaa atgataactg caagttatca cgtaactgac tttcttcaat cagctcaaat
     6781 aacctttctt tcgacaaaga gcctatcttg atctttaacg gtggatagat ttctccgctg
     6841 agaatatttc tttcccaata cgtactgttt ttcaatgtca gtatttcaac aaccggataa
     6901 tgttcgatcc aggggataga aggaataaat gtataaatgg gttgattgtt ttttataaat
     6961 agaaatttcg gttctttttc actgatttct tgataatatt ctgttatttg cgccaggtca
     7021 ttttttaaat ctgtatgtct atatcgttca gggatcaata taacagggtg cgtttctttt
     7081 tcggttattt ggatcaacct tccagagaca tcaatcaaag gaaatttctt aagataattc
     7141 agatttattt ttatccctct gccaaactgt tcattttctt cattcaagta atctgataca
     7201 ttgactaata tacttccgtt tttattcaaa gtttcatata attcctgatc ggctgctgat
     7261 gcaaaacgat tggaataaat aaaatccacg tggtttttcc cgatggttac tggataaaat
     7321 acatgatagt cctcaccagc gatttctttt ggtgtttcat gggaaacagc taactgtgaa
     7381 gaagaaaaaa cgatagttgc taattcgaca ttggcaacgg tcaaaagaag agaaaacaac
     7441 aatccaaatg taaaaagaat gtattggata tagttgaacg cttctcttaa cgaagcgatt
     7501 ttttcaacta ataaaagaga aatcaaagag ataccctata ttccgctgaa caagaaaacg
     7561 tacatctgga acaaataaag aaattgctcc ttataataca taaaaataaa accaccaatg
     7621 attcctgcaa ttatgaatgt aataatgatt ttctttttcc ccaataaaca ccacaatact
     7681 tgatgtaatg aaaagccatt attcaacaat aaactgattt ctttattcat caataagaaa
     7741 aaggatgata acaaaaggaa taaaagaatc aatccaatag ttaaatacac tcgataatta
     7801 tatacgtctg tacttgaaat aaccgaatac gaatagctat tttcaaagtc ttcaaaaata
     7861 ttagtggcat aatcagtgtg aaatgcttga ttgaatcgtt ccacaaatag taaaatgaac
     7921 cgttcatagg cttctttttc tctcgtcatc aagaaaaatt caccttcaaa atcttttatt
     7981 ttgatcgatt cttcaatgga cgttacgcga aaggaggtat tagaataggg taccttctcg
     8041 tgagaagatt tgacatcccc ttgataatac aatgtcattt caagctttgg ctgtatcttg
     8101 aagttccaag atgtatctac ttcattggat tcatttactg tttttttcat ataagaaagc
     8161 tcagtttctg atgagacatt ttcaaatacc atgattagct gctcatattc ttcttgccca
     8221 ataaaatgtt gtggcaatcg aatcggagta taagtatctg tttgatggaa aaattcattt
     8281 gtccgataga gtacctgttt atttataata accaaggcaa aaccactcat gataccggtc
     8341 actatgattg tgagtaaaac tgtgaacttt tttttgatta tcaccactct cccttactta
     8401 aatgattctt tgataaaatc ataccgtttt tcatcaaaaa aacgtatcta tcaaatgtat
     8461 aggtcatgta gaataaatct atatactgta gaatcgaaag gagaagaaga tgaacatcgg
     8521 agaaaaaata cgaacttacc gcagaaattg taagcttacg caaaaagaat taggcgaaaa
     8581 aatcggtgtt tccgataaaa caatctctag ttgggaaaat tctcgtacaa tgccagacct
     8641 tgaaatgctg tcacttttac atcagacttt aggtctgccg attgattttc cttctattga
     8701 atcagctacc gaatcaatag aaccaacatt gccggatgaa cctcttgtta tcaagaaatc
     8761 ttattcatca aaacaaccct tctcactcaa aaagacacta tctttctttc tgctcgtctt
     8821 ttgtttggtc tatctttttc ttatcaatta tcaatataaa aatgaaacgg ttgatcgatt
     8881 caatcctttt ttgaaaccac aaatcggcta taccacactc cctgtaggat acgtggaaaa
     8941 ttccatcgaa cattgggtat gtaatgatat ctttgttcat ggtacatggc tgtcttttca
     9001 cgctggagag gtagaaaaag gtaagaatca tgctgtaatc aaacacaaag gaaaacacgt
     9061 catgaacgtt cgcctgctca cttgggaagc cattccctca gattaccaag aaatcctcat
     9121 ccaagactat gacgatgcct taaaaagaag ccctcaggag ccagataggc attaagtagg
     9181 agatgaacgt agagatagaa aaaatcaatg cacatgatct tatcaaaaaa aaacaggggt
     9241 acaaattaat tgaaaaagct ttgagatgaa agttcctaaa taaaaaagtt cagaatgata
     9301 ggtaaaaaaa tgactaacta ttctagtatt aggctatttg tattattctt ctgttatttt
     9361 tatatcaaaa aatccagaaa taactaaata taggtatttc tggcttatag tataatattt
     9421 acgccacagc ccccaatgat tctttttgga atcatgatca aggacggaca tacacaaaaa
     9481 tattttgccc attcgatccg aatagattaa cgactaatct agtaagtaat atagattggt
     9541 acgtagtcaa accagcaaat ggaaaagttg tctattccac cccaagggct ttaaagacag
     9601 catcttccgg cgtttgataa aaaatcaaat taaaggtacc cattaattct gggggtacag
     9661 ttccaatatc agcagcgctt atcataggta caagtacttt tttcgctccg gcatcttttg
     9721 ctacttgtaa acagttagct aaattatcta ccttaatcat agaaccacca attgtaatat
     9781 cccctaaaat aactaaactt ggtaatacag gtttatttaa ggaaactgag cataaagcta
     9841 ttatagttga taacgataat gtttttgtca taccaatgcc attcatatct tgaacatgaa
     9901 cgacaaaatc ttttgtcgtc aaactaatag actgactaac acttccataa ttagctctta
     9961 aatatcggta tgccgaatcc atcgcttctc ttgctccagc atctgaattg atcccagtac
    10021 gttcaaactt tccatttccc gaagtcattt gcgtttccaa tttataggta cctaattgcc
    10081 cagttttgcc ataagatacc gtgtacaaat gccctggctt ccctaaccct tctggaatca
    10141 aagttcctcc accttgttca ggaacagaaa catactcttc tcgctcttct tctaaatcaa
    10201 tgtatgaaaa attgacatca taaaattcca ttccaccaat tttctttaat tgttctttta
    10261 cacgacgacg catttcaaga gcaaatctta gtatttcttc gacatcactt ttttcaaatc
    10321 taccatcagg atacaacagt ttcatcatcc cagatacaat ccgacgaact gcaatcgtat
    10381 cccgctgatt taattgagaa ccaagtttga aatatttatc aaaactatcg ccaaaactaa
    10441 cttttcgaag ctcacgcatg acttcagaaa aataatctgt aatgaagcca tagtcatttg
    10501 taaaaaaatc tggtcgatat ttaggaattt cccaacctgg taaataacaa tgcatccgat
    10561 ccaaaaaggc tgaatccgtc cccataacct ccggaaatgg ctcaaataaa ttcgaagttt
    10621 tcaatattac atcaacgctt tgattaatat ttcctacaaa agccattgat gctgaagcat
    10681 tcttttcttc tttgcctcta gcaaaagatc ctgaagccat gtaatctttc atgatttgaa
    10741 cgccatcatt atccttaaat ttaatcccag caacttcgtc aaaagcaaca cagtcccaca
    10801 tacctacaag tcccattgta cgactagcca tattatagaa aaggtttgct actgttgttt
    10861 gtccacctga aacaaggatt gagtttggtg aaatttcttt atatacatgc gatttacctg
    10921 taccacgtgg tcctagttca cagagattaa aattattttc aattaatgga ataagtcttg
    10981 ctaaatgaag ccattttacc cttttattaa attgtgtagg ttccatacca atggaccgta
    11041 gaagtaagtc aatccattca tttgtagtaa actccttgcg agccatcttg taagcctcaa
    11101 tgtctaaacc aggcatttgg attggatcaa ccttttttgc aataaaaggg ctattccaac
    11161 gatcattctc atcataccaa tattccatcg aaacaataca ccagatcccc ccacctaata
    11221 atcgatcaaa ttttttcgga tactcttcac taatttgaac attttttaat cccaggttag
    11281 agaattctgc ttcataacaa tctttctttt catttaaatg tacagttact ttatcaataa
    11341 ctgtataacc acccaattca cgaatttttg cttttacttt ttctgcttca tcaggacgca
    11401 catagtttcg agcaagaata tttttaacat tttccacacc tttttcaacc aatgtttcat
    11461 ccaatgacga agcatacata ccaagtaaat attccaatac ataaacaggt acattcgcac
    11521 cttctttaat tttctttgtt aaatcttttc tgacaatctt tccgggaaag aattcagata
    11581 actttaaaat ttcctgttgt tccactagaa ttcctccatg tgttcagtca ttataatagc
    11641 aattaaagta tatcatactt aaccagtagg cttgataata caaaagaaaa agtatccaca
    11701 aaaaattgtg gatacctcgt ttttatatat caaaatcaaa tgattctgca aaaggattat
    11761 ctataatgaa ttttattcgc tcggtcaaat catttttttg attttttatc actaaataat
    11821 aagtatcttc aactgtatat cgacgattta caaattcaaa aacaaattta gtaaaccgct
    11881 gatttgcatc ttctgttttt gaatccgcaa catagatttc ttcatttgag atacgttcac
    11941 catattcatc ttcaaaataa agccagaaag atgcggggtc ataaacatca ctaattgcat
    12001 ttttttgata gaattctaac gtggttgata atcctgtaat tctgcggttg ctagtcatta
    12061 attcaattgc tactggttcc tttgcattgc tacctttggc taccgtaatt tctagaacag
    12121 ggactagcat ttcttgaata gatgaccctc catgaacgta gctttgtccc ccgccagctt
    12181 tcttaaaaat acttgaagta atcgggacac aaatgtttct agtgtcatca tttcctaaag
    12241 aatctcccaa agagtaattt ttcgtaccaa ttatttcgta ttcttcttca ctaagaataa
    12301 aacgacgttc tacacgatct ttttcatttc tagatggatt ttcaatctta tctacttctt
    12361 caacgtggct tcttttataa ataaatccat gatcagccgt aactataaaa cgatagatac
    12421 tgccatcatt atgcaagcga ttaataagtc gggaaatttc gcttatcgct aaatcacagg
    12481 cctcaaaaac tcgattttca ttcgagtgat cacctgttct atccacttga tcgtgataaa
    12541 tataaatgac tgatttgcca ttaaatactt ctcgtacctc agccttattc atcttaacca
    12601 catcatcata atttaaagct acagcatttg catctcgttg ctggagaatc gcatttcgat
    12661 tggataaacc attcgttttt ttcccatcaa tcagtacttc tgttccttca ttgtaagtta
    12721 gttgattgtt tggtaataag gcggctttac cgaattcagt aactgaaggt aatacagaaa
    12781 gaaagctagc catgtttgtt gaatacttct tttctctaac taacttacgt tctaaattca
    12841 tccctaactc atatcgaaaa gcatctgaaa taataactac agttttaaca gagcgtttca
    12901 ttacagcttg attataaaat tttcgaatgg atggacgatt cttaaattca aaagtttgat
    12961 tccaaatttg tcctgattga tcaagaaact tcgaataatt tgcttcaact aatcgataca
    13021 attggccata tttttgcttt tcttctatat gatcactatt ccaaataaat ttccgataat
    13081 gcgtatcaac ccaatacatt tccttctcat attgagttag caaattatcc atgttcatcg
    13141 gcaagcacaa tctgttttta tccacaagat aataagcatg tttcaataat tgatactcat
    13201 aaatataatc cgatgcgaaa tgcatttttt cgcgctgctt taaactatca aaaattgttt
    13261 gtcctgcaat tttcgttgta gtgtcagaag cctttaatcg ttctgcataa aacattaata
    13321 tttttgcatg aatctcagaa aatacgtctg cttcaattag atcatcaatt tctatactgt
    13381 ctaataatcc atcaccatca atagcctgat atactttttt agatagctgt tcaaagcttt
    13441 ccatgtaacg tgtatcattc ataaactgat ccatgaaagt aataatgttt gctttcaaat
    13501 gtagtatttc aaatcctttg aacttcttcg gcatctcttg atcaatttga taataaaaat
    13561 agttcaaata aagacacatc gtcagctttt ccaatgttgg ctggctatcc gtaaagccaa
    13621 atttcatttg aataaattcc caaaatttag ctgttaactg atacttatca agttccctta
    13681 aaggattctc cctactttct gtaaagcttt ttagaactac acgcagtaaa tcatttaatc
    13741 gaatactttt cgatttcaac aaaacagcca ttatcattaa ttcaatcgtt tctgatttat
    13801 taattgtcat atgcgtacta aattcaccaa aacttctcat gcgatccttt gcattaaaga
    13861 aattacgata ctgtttaact gtctgatgaa atctaagtgg aatagccaat tcattcatga
    13921 taatcgctaa gcggtctgca ctaaattcct ctgaataatt caatacactc aacaaatgat
    13981 tatcttcatc tttatcatcc caacgaggaa atggcgcata gattaaatag ttagtttcgg
    14041 gatcttcata ttccaataaa cgcttagttt taaattgtgc gtccttttct aatcgataga
    14101 tttttgcatt ttgaatgacc aatgagtcaa tttcttcaag aaaatcctga tttgaatcaa
    14161 accaaaaaat gattttccta catccataat taggaaaacg ttgatttaat ttttcttcaa
    14221 tttgttgcgt atttatatct gtcatcaatc tcacctaatc gttctctttc tatacccctt
    14281 atattgacat aatttggata atttcgcacg caatttaaga ataaaaaaga aagttgttag
    14341 aaaacaactt tcttagataa ctattttttt ggttctttga ccccttttgc aaaaatttgg
    14401 aatttctcgc cggttttagg atcacgctgc aacttatcat aattgacaat tacaccatca
    14461 tctaagtcta attcaattcg ttttaatgct aagtgaccca acttctcgtc atactcacga
    14521 acttctttta attgcttctg aatctttaca atttcttttg tcattgcatt tctttctttt
    14581 gggttatcac ttgttgaaat ttgttgctga cgcaattcga ttcgattgtc atacgctttt
    14641 tgtagttcgt gtaggtattc agtacgtact tttcctaatt gatctggtgt atagcgatgc
    14701 aagtacatta acgctttaaa gccattttgt tttcctgagt ccaattgcca ataaattggt
    14761 cgattggagt atgtttttaa atggtctttg taaaaatctt gtacgaagta acgacgaata
    14821 atttctcgac ttgtcccttt gccacctaaa gtgtcagcga taaactggag attttcttca
    14881 agggtttctt taccaaaagt aatctcaacg aaatcaatga aacgattgac aatgtcaatt
    14941 tggctatcct tgaaatactc atcatcggtg atgagtaaaa cattatcagt atcaggttta
    15001 aatttttgat agcgggaagg atcccattcg ccgccagcga agatgagacc atcttcgtct
    15061 agggagtaac gaccgaagat acaacctatt gaataagaaa taaaatcctt aatttccctc
    15121 gccttatcag gtaaccttgc agagatatat tcatcttgaa cctcttttgt taattctttt
    15181 tcaagagaat aatgattaat gaatatgtcg ttcagttcct cttcattcga ttttagagta
    15241 ttaaattgat taaccaactg cctacttaga cagttaaatg cctctgagag atctttttta
    15301 aacatataac ttaacaaaat gtttcttcta aactcgtatg acgtttcaag agcattatag
    15361 tcttttctag ctatatcgat attttcatcc actttttctc ttattttttt taattttctc
    15421 tcagatgtaa ctttaagaaa aggtagtttt cccattggtc cttcatggta atccaaggtg
    15481 ggagaaataa aagttaaaaa gtgattaact aatttagagt ttacatagcc aaatatttct
    15541 tcaaagtcag ttatatcctt tgagaacaga acagatcctt tagtttcaaa aattgcctga
    15601 ttctgcaatc taaaagatac ttgtccagat gaaagtgtcg accaagtaat tcccttttta
    15661 aaatagtaat taagattttg tggtctagat tttaacttcc cattttcatt aacataattt
    15721 tttatttcat ctccatcatt ctcccagtta accacgtatt cattgtttcc ataccatttt
    15781 ctaaaacgac cacctttagt tgtaggaaac catttttttc tagacttttt agcgtcctct
    15841 aatgttttag catcaaactt tattttgtta aattctactt caaaccaata ccgcaaaaat
    15901 ttattgttat ttccggttgc caacccttgt tttgcatctg caacatcgtt agttaatttt
    15961 ccttgagcaa aatcagtgat tacgttatcg cttgcccagt aagcaatcgg cattcctggg
    16021 atttttttga agttgtcttg attagtacta taaaaataat cgcattcacc atgctgatga
    16081 attgcttcta acgttttaat tttttgaact tccattccac ctttaaaatc tgataatttt
    16141 atatagcttc catcttgttc atttgaattt tttatcgtaa acgtacaaat tggaacggtt
    16201 gcttcttcaa aagctgaata ctccatttga atcaaactag aaatgtttct ttcattaatt
    16261 aaaaatgagc gtaacttttc atagctagaa atgaacatcc aaacaaatgg cgtcataaag
    16321 gaagcatatc cattatcaat cagcatttgg ctattcaatt taataaacac agaaaataga
    16381 tcttttttga catcagaata gttatcattt acatattttt ttaatttctt atccatttta
    16441 ttaagataag gcggattagt gactaccgct acatatttat caactagcat ttcagccacg
    16501 tcaagaatgt gcaagtattt ttcttgtatt tccttcatcc cataaagaat atccatattc
    16561 gtaaattcgt tgaattttga tatatactct cttacttttc ctaagtattc tttagttatt
    16621 gcctgattat ttttatcgac taacttaatg attgatccaa gttctttccc attttcaaat
    16681 tgatcaaaaa ttaactcttt gtattcattc cagcgttcac tatacttatc attgttcaat
    16741 tcactaatac gctcttgata ctcgtctgtc actttaggag aatcaataaa ctctcttaaa
    16801 ttcggcgata gacttttttt caaaaaaaga cgatcgtatt gacgcccttt catcataatt
    16861 gcaaaataag acaattgata cgctctttta tcgatttcaa gaccatataa attgtattca
    16921 acgattgcac gtgcagcatc acgctcagaa tatcctaacg taagataaat ttccatcaat
    16981 acatcgaagg cgtaaactaa aatatgccca gaccccattg cgttatcgat aatttttagt
    17041 tcttctggtt ttaaattact tacagatgtt gcttgttcaa aagcatattt atctggaatt
    17101 aagtatttca acttatttct taattcacta ttgggataat gatctaacca atactttcct
    17161 aaactattgt caaccatata acgaacgacc caatctgtgg taaatagttg agttgctgcc
    17221 ggaatatctt gttttttgac aatcccttta taaatattca ccacttgatt atgtggctca
    17281 ctattatagt actgatacaa ccacccaata atctctacct gtccaccagc ttgaacatag
    17341 aaatcatcct ctggaatact taaaagatca gcgattacac catctttttt aatataagaa
    17401 gctgtaaaca aaagttctgt ataatcttct acttcttcaa atagctcagg taagtaatca
    17461 ttcaattgat tacagatttt tataaaaatc aattgataca gcttgtccat tgcttctgct
    17521 gaattatctg ttttccaagt tctgacttgt tgctgttcat tactagataa ttcttcaaag
    17581 atatcggcat cataaatatc tgtaataata tctggctcaa ttttcccact tatttcacta
    17641 gataatacac gatacgtgtc aggtaaataa tcatttacct ccataaaccg aatggcaatc
    17701 aatcggttaa accacgtata agcaacctct tcgattaact cttgaaaagc ttctgaataa
    17761 gaagtgacag aagcacgttg ttttagttct gcaatcaatt tgtttctttg cgctatttct
    17821 tttccttgaa ttcttattcc tgatttttcg atttcttgta aattgctatc aattttcttc
    17881 acatcttcaa tttttgattc atcaattcct aaacgttttg ctttagtcat tacttcatca
    17941 attaattttc tacgggcatt gaccgcgaaa ttctttatcg ctttcttatc catttttctt
    18001 tcccttctat atgtaacctt tgaaaagaca ggcacctttt tatgcctgtc ttttcactag
    18061 aattgaatcg tgatttctgt attttcttga agttgttcct tcagtttttg attaatttca
    18121 gctgttaact catcaatatc tgctgctgac ttaatagttt taacatgaac gttaagtgtt
    18181 ctcattggta ctgttttctg ttgaatagta ggttttacta tcgctatttc ttcagctttt
    18241 gctgcagagt ctatttcatc tttttcagtt tcacgaatca agtttgctgc ttcaatttgt
    18301 ttacgtgctt gttcttcttg acgtctgcgg ttttcttcat tatcgaaacg ttgattaaag
    18361 atcaaatact gttctgctac gtttaatgat aagaaaacaa gatcatctat agtattcgct
    18421 tgttcaattt ttgctttcag ttcttgaaat tcttggtcaa ctattggtac aaattgatcc
    18481 ttaaaatctt ttccttctaa taatttaaat gttgcttgtt tttcttccgt taatttattt
    18541 aatatttcct gactttccaa ctctaacagt tgattatatt gttcaataaa ttcttgaatc
    18601 aactcattta gtttcatcat atctgaataa ggatcagcct ttttcaaaat ggttttcatc
    18661 tcatgaatca attgatcaat ttcttggcta gaagtttgac gccgactttt ttcgattaat
    18721 ctaattttat cttgtgcatt ctcccagatt tttttctgag tactattttg cttgtggaaa
    18781 tttttaattt tatcatatgt ttcagcccag tcgaataatt catcgcgata ttttttgatt
    18841 gtataaaaaa agtcttcaac atctgacgtt tgaacaatta cttcaagata tttttttatt
    18901 tgctcatatt cataagcact agggtaggta taagtgtttt gtaaatcaaa ataagagcgt
    18961 aaattgtcta attgaccttt taaatatttc ttgaaatttt ccattgtatg ttcattgtta
    19021 ttttcatcaa caatattacg actttgaaac agttctctac cgatttcgtc aaccaatttt
    19081 tttgcttttg gcgaaatcac cgcgcttggt tcaattttta tcttttcact ttctcgtcgt
    19141 ttttgaataa tcattacaat actatttgga tcatccatcc gtgaaatttg tacattgtta
    19201 taaagtaaat caattttacc agacatgaaa agtttagcga tgcaccattc cacatcaagt
    19261 tctgtgaatc catacggttg ttttgtttca aaacgatcac gaattgtttt catcgtaatc
    19321 gttaggtgat ttctcttttg atcattgata aaattgaaaa tttcctgaac tgcttgctca
    19381 tttgatggat cttcaataat ttcatttttg ctttctttca ctaaatataa gatatcttct
    19441 ttttctttag gcgcatcaat ttggtttaat ttatggtata cttcttcagc cattttctga
    19501 agtgctgatt ggaattttgc tttaaagtcg gagcctgtca gttgaatttc tgcaccgtta
    19561 tagaaaaatt ttgcagattt caacagttca tttaattgat cgttgatact tgcacttagt
    19621 tcctgtaatt caccagattt ttccgaacga atcgcgtcaa agttctttac tacactcgaa
    19681 taagtcgttg ttaaataaag attcattcta gcatacattg ctatatcatc tttaaattct
    19741 ttatctgatg gcaattctac gaataataag ttacgggaac tcttaagcgc aagttctgcc
    19801 tcttgacctg ctaaatcaga gtatggtgaa ataaaattaa ctgtcatttc agcatttcca
    19861 gcttgcttgt atggacgttg atctaacact tgtgcataag taaacgtata ccggttttta
    19921 aatttatttc cacctgacat gcctaatttt tcataggcaa aactatttcg gtctagaacg
    19981 ccgtcaaaaa ttcgtttaga tagttctacg atcaaatcac tatctgtaat attttgattt
    20041 ttaatctcac gagtaatttc ctgttcttca tcagtttgga aaatataact atctaagttc
    20101 ttccgcacaa agttttcctt aattaaaagg tccaatgctt cttctactcg ttttttagtt
    20161 tccaggcggt cttcgttgat atttcttatc atcaagctcg taatattttc aacatttggt
    20221 ttgatggctt gatcaacata tttaatcatg aataaaattc gtaagacatt tacgttaaag
    20281 ttatctatat cagttggtct ttgaatatga gaattatttg tagcttgtgt aatcactcgg
    20341 ctaattgctg tatcaatcca acgttctaac gagtaataaa actgatctaa agaaaccaaa
    20401 ctggttgtat cattttcctt atttcgttca gcggcttctt taaacattgc tagtaatgaa
    20461 cgttcaccag acgataagtt tgatccttga tagctgtgat tacgaatctc ggttagaatt
    20521 tgccccaata aattgaattg atagggaata aatggataaa cttctgcgta attttcagca
    20581 tcttggaaga gctttaattc aggactatca acaaagagta atttattttt caattctgtt
    20641 tgaacagttt caaattgtaa ggctaaagaa tcattggcaa actctttttt cttcaaaata
    20701 cgttctttaa ttacttcatc ggcatttgct gaagataaac tcacagttgt ggcaaaacga
    20761 tccttgatct tagaaaaatc attatcagaa ttgttatcaa agtttgttac gtgatcaatc
    20821 gcttcctgac ttgtcactaa tacccaagcc ttaccttcac aaattcgacc caaatcttcc
    20881 acaacggttt gtaactgcaa agtcaactca cgattaccgc cgatatattg tccgacctca
    20941 tcgactagaa aggcaatatg atggtctgct tctttggaat caagatagct tttaactaga
    21001 tttgcaaagt catcaatgct tattaaataa tttcctgatg ctttttctaa aaaattatca
    21061 atagcggctt cactcataaa atcgatttta agtgccgctt ctttgatatc atcttggatc
    21121 caatcaaact catggcgctc ttcttgccat tttgccccat taatctcttc aaaagcagct
    21181 ttgaatgcat caaacttttc ttctttaatt aaccgacgtt caaaatctgc tagatgtgga
    21241 atcgtgctta atccttgcat ttcgttaaat acctttaaaa aaacattcac gattccgttt
    21301 ttgtcttttt taccacctgc atcacttttt gaatcaatat taaacagaat aacatctgta
    21361 tttatttgtg agcaccgttc catcgttgcc aaagtcagtg gatctttgat tttcccatct
    21421 tctttgaagt aatcaatggc tttcttgtca tttatttctc gatttgcgag caagtaagaa
    21481 ataattttta agaaatgaga tttacctgaa ccaaagaacc cctgaatcca aacacccatt
    21541 ttatctgttg gtgtattgat actccgttgg taggcatcaa aaattgtttt aaagtgtttt
    21601 tgtagttctt tcgtaaccac gtattcttct aattcttgtt gaacattttc atcctttgct
    21661 tgaccgacct taataactcc ttgaatattt cggtcaattg gttttgcaaa aatatcttta
    21721 attagcattt aatctcctac cttccatccc aactaaattc taaatgctcg ataataatta
    21781 tcatctttat tggtattaag aattgtcaaa cttaattcat catattcccc tggatacatc
    21841 atcacaactg gtacgcgatc aaatacctga tgcattttat ttaaaataga gtgcgctcta
    21901 attaatggat ataattcgcc aattcctgtc aggaacacaa ctgatttttc tggtgtatgg
    21961 gcatccacat attcaaaaaa tacattttct tcctcaggta aatccatttt caataaacga
    22021 ccaatgcttt ccactaattt ttgtaaacca cgtcgttctt ccatcttaat cgctttttca
    22081 gtgaaaccgc gtttttctag atactctaac ataatcacat agagattata aactttcact
    22141 tcataattat caccaacaga tgttccagat atatcattaa tataatctct tacaattaac
    22201 tcgtctttcg gatcgtagtc aaagatataa tagcctactt cattacctaa accacgattc
    22261 aataaaaaat cttctttttg aattacttca ttcaactttt ccaaatgctc ttgaatcgat
    22321 aatgacatta taaaattcct cccagtgcta gtacaatatc tttacgattc ttgtcaacta
    22381 accattgaat caatcgttta tctaataata tcggagtcat tcgtccatat tctttttccc
    22441 ctctgactaa tcctgtttca attagattcg tttgataagc ggcaataagc cgtttaatcg
    22501 tataatcttt ccattttgaa atatcttctg aacttctccg cttctctgta aaaaaagaac
    22561 ggtaatcaac tggtgttaat tgcaaatttc ctttacgtag attttcagca tatacctcag
    22621 tcataaattc attaaaaaga tcatcaatca taaggatgga aatcaaattc actaactttt
    22681 gattatctat atttaactga ttgaaaagtt gcaatccagc ttcatccagt tgactaactc
    22741 tccttttcac gttctgatag atttcttttt gacggttact gctcactgca aaaaaaatat
    22801 tttcatgttc atttttctct ttaatttcta tatctgtata tccttctagt cgtaattgaa
    22861 tatataactt gaactctgaa aaccaaaagc gtttagaaac tagaccagca gaatattttt
    22921 tattcaatag attgcctcct aatcatctat aattcaataa gtaaacaagt gttttttgct
    22981 catcgttagc attcattttt tagtatctac tatacaatac atatttatta ta
//
LOCUS       DANKBF010000044        22805 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000044 DANKBF010000000
VERSION     DANKBF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            91..501
                     /locus_tag="PWY89_001816"
     CDS             91..501
                     /locus_tag="PWY89_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2066853.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            494..1195
                     /locus_tag="PWY89_001817"
     CDS             494..1195
                     /locus_tag="PWY89_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2066854.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1195..2781
                     /locus_tag="PWY89_001818"
     CDS             1195..2781
                     /locus_tag="PWY89_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066855.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2800..3021
                     /locus_tag="PWY89_001819"
     CDS             2800..3021
                     /locus_tag="PWY89_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL2066856.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            3018..3779
                     /locus_tag="PWY89_001820"
     CDS             3018..3779
                     /locus_tag="PWY89_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL2066857.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4159..4668
                     /locus_tag="PWY89_001821"
     CDS             4159..4668
                     /locus_tag="PWY89_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2066858.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4743..5282)
                     /locus_tag="PWY89_001822"
     CDS             complement(4743..5282)
                     /locus_tag="PWY89_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2066859.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5422..6033)
                     /gene="rpsD"
                     /locus_tag="PWY89_001823"
     CDS             complement(5422..6033)
                     /gene="rpsD"
                     /locus_tag="PWY89_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2066860.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6295..7146
                     /locus_tag="PWY89_001824"
     CDS             6295..7146
                     /locus_tag="PWY89_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2066861.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7174..7809
                     /locus_tag="PWY89_001825"
     CDS             7174..7809
                     /locus_tag="PWY89_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL2066862.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7829..8602
                     /locus_tag="PWY89_001826"
     CDS             7829..8602
                     /locus_tag="PWY89_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2066863.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            9010..9807
                     /locus_tag="PWY89_001827"
     CDS             9010..9807
                     /locus_tag="PWY89_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL2066864.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9785..10198
                     /locus_tag="PWY89_001828"
     CDS             9785..10198
                     /locus_tag="PWY89_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2066865.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10182..12827
                     /gene="mutS"
                     /locus_tag="PWY89_001829"
     CDS             10182..12827
                     /gene="mutS"
                     /locus_tag="PWY89_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2066866.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12845..14953
                     /gene="mutL"
                     /locus_tag="PWY89_001830"
     CDS             12845..14953
                     /gene="mutL"
                     /locus_tag="PWY89_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2066867.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14975..15535
                     /gene="maf"
                     /locus_tag="PWY89_001831"
     CDS             14975..15535
                     /gene="maf"
                     /locus_tag="PWY89_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL2066868.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15620..17608)
                     /gene="treP"
                     /locus_tag="PWY89_001832"
     CDS             complement(15620..17608)
                     /gene="treP"
                     /locus_tag="PWY89_001832"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL2066869.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17831..18544
                     /gene="treR"
                     /locus_tag="PWY89_001833"
     CDS             17831..18544
                     /gene="treR"
                     /locus_tag="PWY89_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL2066870.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18621..19067
                     /gene="msrB"
                     /locus_tag="PWY89_001834"
     CDS             18621..19067
                     /gene="msrB"
                     /locus_tag="PWY89_001834"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2066871.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19237..19839
                     /gene="ruvA"
                     /locus_tag="PWY89_001835"
     CDS             19237..19839
                     /gene="ruvA"
                     /locus_tag="PWY89_001835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2066872.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19852..20853
                     /gene="ruvB"
                     /locus_tag="PWY89_001836"
     CDS             19852..20853
                     /gene="ruvB"
                     /locus_tag="PWY89_001836"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2066873.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20882..21460
                     /locus_tag="PWY89_001837"
     CDS             20882..21460
                     /locus_tag="PWY89_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2066874.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21512..21994
                     /locus_tag="PWY89_001838"
     CDS             21512..21994
                     /locus_tag="PWY89_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL2066875.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22111..22485
                     /locus_tag="PWY89_001839"
     CDS             22111..22485
                     /locus_tag="PWY89_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066876.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7422 a         4076 c         4934 g         6373 t
ORIGIN      
        1 caaaaattgt atcttagaaa cagcatgttt tgaaactttc agaaaaggtt tgttagtaaa
       61 atgaaagcta tacgtaagga gtgttgtttg atgaaatttt ttcaagagtt tttcattatt
      121 attttctttt ctgtattagg agaaggcata cggatttttg ctcatttacc tattcctgga
      181 agtatcttag gtattctttt tttattttta gcatttgaaa tgcatcttgt tgatcctgaa
      241 aaaatcggag caactggtga ttttttattg aataatctag ccattttatt tgttcctgct
      301 ggtgtaggtt tattggaata ttttgatgat attgcttcga tatggcctgt tttactggga
      361 gcagtcgtcg tgtgttccat cgtcacgatg gttgcagcgg gaaaaacggc agaaggtgta
      421 gaagctttgc tgggctatgt ccgtaagaaa aaagcagaaa gggtatctgt aaacgaaatg
      481 gaggagaatc aaattgactg agttttttgt gaatccatta tttggcttaa gtttgacttt
      541 aggagtgtac gtattatctg acaaattttt gcggaaatat aaactgcctg ttttgaatcc
      601 tctcgtatta tcgattgttg ttattatctt atttttgcat ttttctggga tcagctataa
      661 agactataat atcgggggca attttttgac tatgctgatt actcctgcga cagtggcact
      721 tgccatccct ctttataaaa acttccgttt actgaaagag aatttttatc ctgtaatggc
      781 tgcgatttta gcaggaatag tcgcaaattg cttggttagt gtagggttcg gttacttctt
      841 ctctcttcat aaagagatgg tgatctcatt actacctaaa tctgtaacga cggctatttc
      901 tgtcgattta tctcatacga tgggcggggt gaatgccgtg acacttgcta ttgttgtctc
      961 tacaggtatt tttggttcgc tgatcgccac tcatattttc cgcctattca agatcgaaag
     1021 tccagttgct cgcggtgtgg cgttaggaag tacttctcat gctattggta cagcaaaagc
     1081 aatcgagatt ggtgaaatag aaggaatcat ttccggactt gcgatatgcg tcaacggaat
     1141 attgaccgtt ttattgctac cgttattctt tcagcctttt gctggcttgt tttgatgcga
     1201 aaaccactat tttatgctac aataaccaaa acgttcgagc ataaaaaagg agggaaagac
     1261 gtgtatttgt cttccgagta tcttaaacaa tttgataaag atctatatta taagattctc
     1321 ctcctagaaa gttttgagga tcaagcttgg cataccgctg cacaattagc gcaagttgtt
     1381 cagctggatg cccgcagcgt gtcgaaatac ctgaacgagc tgtctaaaaa ctatcagcag
     1441 tttagcggta agacacaccc tttgtttaca aaaaatcacc gttctggtta taacttttat
     1501 gatacgctcg attcaattga acatgagcgt tttttgatct atctcgtgca aagtacgctg
     1561 aaattccagt tgctgcacga catttttttt gaagaattcc atacaatgta ccaatttgcg
     1621 caaaagcatt acattagtga atctactgct catcgaaaaa tcaatgaatg gaaacagcaa
     1681 ctgcaaactt acggaattcg gctgcaacga ggtacatata tcgctcaagg cgaagaagaa
     1741 atcattcgac tttacttgca tatgacattc tggcagttgt ttcgagggaa aatctggcca
     1801 tttgaaacga tttctcaaat ggacgtaaag aatatggcag aacatatcat ggcttttttt
     1861 aatgttcgtc tgaacgagat caaaaaaaga cgattggaat atatgctagg tgcctttttt
     1921 ttaagaaaat cacagaagca ttatgttgta ttgaatgaga aaaaaagacg gctgatttct
     1981 gacaatctgt tgttccagcg attttgccag gtcatggagc cggtatttcc taattatttt
     2041 caagtagaag acgagttagg cgcattattt cttgtactga tgactcgtga agaatattat
     2101 agtgatccta agatacggaa gaaaattttt gattttcatc aacaagcaaa aacaccgccc
     2161 tttacagccc tttcagaagc aaaagccgct ctttctcttt atcaggaaga gcaggggctg
     2221 ccagcagaaa atctaacctt cgaggcagaa aactatttat tcagcagtca ttttttcgcc
     2281 tatctttttc ctaatgcgaa agaaacgatt gatggcaata gtagtgattt tattaatcat
     2341 ttagttattg aaaacaagga attgaaacaa tggctcgtac atttttttga aagtagacac
     2401 aaacatccca atcatctggc atttaaaaat catgcgtttt tgatggggcg gtatctgact
     2461 gtgtttaaaa cattaggagc ctttactcca cagcttccaa agatcacgat ccttttaatg
     2521 acagatttcc cattatttga agagcaactg ttagaggagg gattacggac atttttccgt
     2581 aatgagtatc agctgatttt tctgccaaca gattatcggg ggagagaagt tgatcttctg
     2641 atttcgacaa gtaaagtcca ccgcaaacca tgggcggatc ttgactattt tattgtgact
     2701 gaagaactaa aattaattga ttacatccaa ctatctcaaa aatttgaaat gattcaaaaa
     2761 caaaaactat taaaacaata acaaagaagg agaaaaaaga tgacatttga agaaatttta
     2821 ccagaattga aaaaaggtgc aaagattatt cgtaaaggat ggagtggatt tgaactttat
     2881 gttgttctcg tatccgatga tgaatttgat ggctgtccgg tgacgccgta cttattgatc
     2941 aaaacaagtg acgaaggttt ttccagcttc gctcctacag tttgtgatat tttagcagat
     3001 gattgggcga tcgtagaatg aaaaaccagt atccagatct agctaaacag gttgtattta
     3061 tcacaggagc tgcttctgga attggtgctg cgcaagcccg agcgtttctt gaacaagatg
     3121 cttttgtatt tggattagat caaaagttcg gtaaaatgga agaaatttct ttgcgatttc
     3181 ctgaaacttt tgcctacgca ctaggtgatg ttcgacaaat gaaagaactc caacaagcgg
     3241 tatcgaaatg ccaaaatcat tttggtgaag ttacgatttt actgaataca gcggggatat
     3301 tggatgccta caaacccttg atggaaactg acgaatcgct gtgggatctg atttatgaga
     3361 cgaatgtgaa aagcatgtat cagctgacaa aaatcatcct tcctgatatg atccgccaaa
     3421 aaagcggaac gatcatcaat atggcttcga ttgctggctt gattgctggt ggaggtggga
     3481 tcgcttatac aagcgcgaaa catgcgattg tcggatttac caaacaactc gctctggatt
     3541 acgcaggaca gggtatccga gtaaaaggaa ttgctccagg agccatccaa acaccaatga
     3601 atgcggctga ttttgaaggg aaaggtgaga tggcagagtg ggtggcaaat gaaacaccag
     3661 tcaagcgatg ggcacagccg gaagaagtag ctgagctgac gttatttctt gcctctcctc
     3721 aagcaagcta catacaagga acgatcatcc caatcgacgg cggatggttg ttaaaataac
     3781 cttgtgtttt tttcaagctt ctgtatgata aaactaaaga gagaaacaac ggttgatgat
     3841 aaaaaaagtc gatgactgtt cccgacattc gatgccgaac agccagttca aaaaattctt
     3901 tttaataatc gttaatattc gaaactaagc ggcttttcac gacctcttga tataaacgat
     3961 attcaaaagc ttgcaactgc ggtaacaagt ccagccaaat gaaaaatata aggagctatt
     4021 tttatttgac cattcttatt acttgttgca gggcagcttt ttcactacct tctgcgacac
     4081 ttcagttgga attttcttcc catcctttgg tcgcggtcat taccaagata aaattatctt
     4141 gcaaaggaga aatagaacat ggaaaaaaat aatacccgtt cagccaaacg ctggacaacg
     4201 acagatgtga ctcggatggc gctagtcact ggtttatatg tggcagtgac gctggttttg
     4261 tctgtcttta gctttggagt gattcagatt cgtctctcgg agatgttcaa ctatctgtcc
     4321 ttgtataaca aaagatacat cgtcgcggta acagcgggag tagctttggc taatattgct
     4381 tctcctcttg gactgataga tgttgcagta ggaagcgtca gcacgctgct tgtattgctt
     4441 atcaactata aaatcactag tcgcatcaaa aacatgaaaa tcaaaatggc cgtgacagca
     4501 tgcgtatttg ctttttccat gtttacagta gctggtcagc taacgatttt atatcaacta
     4561 ccttttttct tgaattggtg ggtgattgct ttaggagaat tggcatcaat gattgtcggt
     4621 gggataatta tttattggat cggtaaaaaa gtggatttgt caaaataaag atctgtttaa
     4681 tttaaaatag ggcagcatgg aagagactga atgttgagtc ttcttccatg ctgctttttt
     4741 gattaatttg tatgaagaaa tcgatttagc cgttttttca tcaagatacc gatataagct
     4801 gctccagttg ccttgattgc ttccggcagt aaaaacggca gtagtccact ggcaaatgca
     4861 gttgtaaaag taagatcacc acttattttc agccagatcg ttccgcagat caatgcagca
     4921 agagagccca ttagattggc aaagattgca tataagtaat gagcatccgt tctttcgatc
     4981 atccaaccag ttatccacgt ttgtaagaga aatccaatca aatatccgcc agttggccca
     5041 aataaaacag ctatacctcc agtcatccct gaaaatacag gaagaccaat caagccaagt
     5101 aaaagataaa ctccaacaga tatcaatcct agttttcttc cgagaaaagt tgctgttaat
     5161 ccaatagcaa atgtttgccc tgtcaaagga accaaaccaa aaggaatggt catctgagaa
     5221 aggacagaaa taatcgcggc aaattccgca gcaagtatca attctttcaa tgttcggttc
     5281 ataacgccct cttttcgtta acttaatatt tttatttggt taacgaaatt gtaaaacaaa
     5341 gtgattactt tgtaaataga aaatataaaa aaagaccagc tatccaatag atagcggtct
     5401 ttttcaagaa agcttttgaa attataagct ttggttgtag tattcaacga caagagcttc
     5461 gtcgatttct ggatatagtt cgtcacgttc tggtaaacga gtcaatgaac cttccaattt
     5521 ttcagcgtca aagcttacga atgctgggcg tccaacagtt gcttctacag cttcttttac
     5581 tgttacgatg ttttgtgatt tttcacgaac tgagatcact tgacccactt ctacgtggta
     5641 tgaagggata tcaacgcgtt tgccatcaac taaaatgtga ccatggttaa ctaattgacg
     5701 tgcttgacga cgagtcgttg caagtcctaa acggtaaaca acattgtcta aacgttgttc
     5761 tagtaagatc atgaagttaa caccatgttt accttctttg attttgctag cttttacgaa
     5821 caagttacgg aattgacgtt cgttcattcc gtacatgtga cgaagttttt gtttttcagt
     5881 taattgcatg ccgtattctg attgtttgcc acggctatta ggtccatgtt gacctggtgc
     5941 gtaagggcga cgtgctaatt cttttcctgt gcctgacaat gagatgccta agcgacgaga
     6001 aattttccat gatggtcctg tataacgaga cattaaaatt tcctccaatg aatataagtt
     6061 tttggagtaa aataatccgt tgaaaaattc ataatcatat agtttgttct tcaatcttca
     6121 cctttgcagc cgcggttaca caatcgaacc cgagacgggg caacaaacta gggatgagtt
     6181 attatttttc tgctgcatta ttttacacaa attacagtat atgatagatc atccacgaat
     6241 gtcaagataa ttagtacata aaaacgcttt acaaacctag gctgtcttta taaaatgaga
     6301 gggtatgtgc aaatggaaaa aagcgaagga agtttaagga gaaaatcaat gaatccggta
     6361 tcaccattat ttgagaaaat cgatgcttca attgaaaaga aggcgaatct ttttgaaaac
     6421 agcttttcac gttacgctgt tcgagcggtt ctcgcctgtt tgtttttgac tttaggaacg
     6481 gctgtggcat ttgcgatcgc gatgaagggg gaagatatcg cgcatggtct aggaaagatt
     6541 ttatacgcat ttatgttcag ctggggtctg gtcatgattc tatacatgaa cgccgaatta
     6601 ggaacatcga atatgcttta tatgaccgtg ggtgtttttc ggcagagact tgctttaagt
     6661 aaggcagcca agattctatt tgcctgtatt tttttcaata tgattggcgg tatattatgt
     6721 ggatttctaa tttctatgac tacgccattt caagatttaa cgttggacaa ctttatgtta
     6781 gaatcaatag cagtaaaact agcaaaacct tcagcaacga ttttggtgga agggatgttt
     6841 gcgaatatcg ttgtcaatac cgctgtactg atcagcatgc ggatgaaaga ggatgtcgga
     6901 aaagtgatca cagtcgtatt catcattttc atttttgctt ttttaggata tgaacacgtg
     6961 atagcgaatt ttcctgcctt tacgcttact tactttgctt ctcacggaca gatggatgga
     7021 atgacagtca gcaatgtgtt acataatttg ttttttgctt tggcaggaaa ttatattggt
     7081 ggcggtttag taatgggact gggatatgct tggctgaatc agtcaaaatc atcctatgtg
     7141 gactaaaaag tcgttcatta aaggggaagt gccatggaag ttttagtttt tccaaaaaat
     7201 gaggaaaaag cgttacaaat ggaaaagtac atgaaaaatc ttttttcctt tgctggagta
     7261 cctaagccgg aacgcgctct tttacaacaa ccgttactgg atgagagcaa aaatttatct
     7321 atttctgaag ttttagcttt tgttcattat tattatgaac aaatcgaaag agagtatcag
     7381 tatgtagcga tcgatttagt ccagaaaaac atcaaacggc ttacatttga agacttagtc
     7441 gtattgttgc cactagttca gaaaaaagaa tggtgggaca gcatcgactc ttggagaaaa
     7501 gtatacagca catggtgtaa agcctaccca gagcagcgtg aagaagtctt tcaattgttt
     7561 gaaaaaaagg aagatttttg gctacgtcga atagcaatca cgttgcaatt aggttataaa
     7621 cagcaaacag acacaggact attgatccga gcaatagacg aagatcgtca aacaaaggaa
     7681 ttctttatcc aaaaagcaat tggttgggca ttacgcgatt atagtaagac cgatgccgat
     7741 tgggtaaaaa gacagctgac aacagatttg agtaagttag ctgttcgaga ggcaagtaag
     7801 tatttataag aaagcgagac gacaagagat gagacgcaaa ttatttgctt ttgatattga
     7861 tggaacgttg ttagattcaa acaaacaagc tttagatagc acaagagaag cattggaaaa
     7921 attacgcaaa gccggtcatc tggtcacgat tgctacaggg cggagccgat ttcatgcgca
     7981 agagatcatt cgcgatcttg attttacgaa ctacatttta tgtaatggag cggccggttt
     8041 tttagaccat gaacaagtat ataagaattt actagatcgc gaccagttgg atgcgttcgt
     8101 tcaagaagcg catcatgagc agatcgatac agcttttgtc agcttagaca atatcaagcg
     8161 ttcttcttcc aatgatgtaa cggtgatgga agaagcgatg cattctttcg gtgctgtctt
     8221 gcctgaatta gatcagtttt ttgcagagga acaagaaata taccaagcgt tagcattttt
     8281 cgatcaaaca tatgatggga agttctcttc atatgataaa attcgatttg tccgctggca
     8341 tgagaacagt gtagatgtcg tacctaaaaa tggttctaaa gcagcgacaa ttctgcactt
     8401 agcagaacaa gtaggcatcc ctcatgaaga catcatcagc tttggcgatg gacaaaacga
     8461 ccgtgaaatg ttgcgcatgt caggaattgg gatagctatg gggaatgctg tgccagaagt
     8521 acaagcagaa gctaagatgg tcactgatac aaacgatcag gatggaattt ggaaggcatt
     8581 gaaagagtta agcgtgattt aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa
     8641 gcccagccaa acaaaaatag ctcctcctat ttttcgtttt tctgggctta ttaccgcaga
     8701 tgcaagcttt cgaacaccgt ttaaggaaga ggtcgtgaca gaagtggtca gtttcaagaa
     8761 ataagaaggg atttacgaaa attgcttctc aaatttttgt aaaattacgg cttatttctg
     8821 aagaagtaga tgaaacaacg tttattcggg aaatgggact gtgacaagat gtttgtcata
     8881 gtcccatttt tttattttat ttttcaaaac taagaaatac caggaaagag ttccttttgt
     8941 ttgacctttt ctttcttatt gaaaacaact ataatataga gtgatgttaa aagagaagag
     9001 gaatttagtg tgcgggtatt gtttattggt gatgtagtag gttcaatggg acgagagatg
     9061 attactgaat atcttcctag attgaagaag aagtaccgtc cgcaagtaac gattgtaaat
     9121 ggtgagaatg ccgcttctgg acgtggaatc acagaaaaaa tctataaaaa atttttgcaa
     9181 gacggcgtag atgtcgtgac gatggggaat catacgtggg acaaccgcgg aatctttgag
     9241 tttgtaaatg aagctaaaaa gatggttcgt ccggcaaatt ttccggaagg gacgcctgga
     9301 caagggatgg tctttgtcaa agtgaaccaa gtcgaactgg cagtgatcaa catgcaggct
     9361 cgttctttta tggtggactt ggatgatcct tttcgtaaaa tgaatgagct ggtagaagaa
     9421 gccagaaaac gtacgccgat catttttgta gattttcatg gagaaacaac aagtgaaaaa
     9481 caagcaatgg gctggttttt agatgggaaa gtttcagcag tggtcggtac acatacacac
     9541 gttcagacaa atgatgcgcg aattttgcca agaggaacag cgtatctgac agacgttggg
     9601 atgacaggtc cctatgatgg tattttgggt atgcgcagag aacctgtaat cgaaaagttt
     9661 ctgactgcat taccaaaacg ttttgaagtt gtggaacaag gccgatcgat tttaagcggc
     9721 tgtatcttgg aattggatga tacgacaggt catgcaaaag aaatccagct gattcagatc
     9781 aacgatgatc gaccgttcat ggagtagaaa gagaggcagt tttttgcagc aggagccgga
     9841 aattcaaaaa gaagtaagga aactgacaaa acttttaaga gagaatgaaa cgatcattcg
     9901 ctacaaagaa cttgaagaaa agatccagca aaaccagtat ttagctgagt tgagagaaaa
     9961 aatcaaacaa gcgcaaaaag atgctgttca ttttgcccac tatgacaaac cagcagcaga
    10021 aaaagaagca atcaaacaag cggatcaatt catgcaagaa tttgatcagc atccgcttgt
    10081 tgtagcttat agaaaacagc tgttggaagc agatgatctg cttcatcatt taacaactat
    10141 gatacaagaa gaaatcaatg gacaaataga ggaggaaaaa catgcctcaa aaaactaaaa
    10201 atactcctat gatggaacaa tatctaagca tcaaggccca ataccaagat gcttttttgt
    10261 tctatcgttt aggagacttt tatgaactat tttacgaaga tgcgatcaaa gcgtcacagc
    10321 ttttggaatt aacgttgacg agcagaaatc gaaatgcaga agaacctatt cctatgtgcg
    10381 gtgtacctca ccatgcagca caaggatata tcgatgcctt gattgaaaaa ggctataaag
    10441 tagcgatttg cgagcaggta gaagatccaa agacgactaa agggatggtc aagcgcgaag
    10501 tgatccagct agtcactcct ggtacagtca tggacagcaa aggattgtcg gcaaaagaca
    10561 acaatttttt aacagctgta gttagtcaag gaaatgaatt tggctttgct tatgccgatt
    10621 taagtacggg agaattgaag acggctcggt tgcatgatga agaagctgtt ttaaatgaag
    10681 cctctgcatt gcagacaaaa gaactcgtat taggcagtga gatcactgat acccttcgtg
    10741 aacagctaag cgggcgttta gggctagtct tttctcaaca aaatgagatg gaagaaaacg
    10801 cggaatttag ctttttgaca agcgaactag tcgatccgct ggaaaaagaa gcaaccggca
    10861 aactattgac ttatttatcg gtgacacaaa agcggagttt agctcatatc caaaaagcag
    10921 tagaatacca accagatcat tttttgaaaa tggattatta ttcaaaattc aacctagaat
    10981 taagccaatc tattcggact gggaaaaagc acggcactct tctatggttg ttagatgaaa
    11041 caaaaacagc aatgggtggc cgtttactaa aacaatggct agaccgtccg cttatccagg
    11101 caaaacaaat taaagcaaga caagaaatgg tcgcttcttt gctagacagc tattttgaac
    11161 gtatcgattt gcagtcagca ttaacgaaag tctatgatct ggaacgccta gcaggtcgag
    11221 tagctttcgg taatgtcaat gggcgagact tgatccagct gaaaacttct ttagaacaag
    11281 tgccaatgat cagagggttg attgaaggaa tcgatcaagg tcaatggcaa agtctgcttt
    11341 cagaaatgca gccgatggac catctcgtcc aattgatcga ccaagctatc caagatgatc
    11401 cgccgttaca aataacggaa ggaaacatca tcaaagatgg attcaatgag cagctcgata
    11461 tgtaccgttc tgctatgaga aacggaaaga aatggttggc agaacttgaa gcaaaagagc
    11521 gtcaagaaac aggaatcaag aacttaaaaa tcggatttaa ccgcgttttc ggttattata
    11581 tagaaattac aaaagcaaat cttggaaatg cagaactgga gaagtatgaa cgaaaacaaa
    11641 ctttagcaaa tgcagagcgt ttcatcacac cagaactaaa agaattggag acacaaatcc
    11701 tcgaagcaga agaaaaatct gttgatttag aatatcagtt gtttttagct gtgagagaag
    11761 aagtcaaaaa ggctatccag ccgctacaag tacttgcaaa agcaatcagt gcagtagatg
    11821 tgctacaaag ttttgcaacg atcagcgaac gctatcagta tgttcggcca gaacttgtca
    11881 gtgacaagca tcagcttctg atcaaagacg gacgacatcc tgtagtagaa aaagtattag
    11941 gacatcagga atatatccct aacagcgtag aaatggcaga agacgaaatg atcttgctga
    12001 ttacaggacc gaatatgtct ggtaaaagta cgtatatgcg acagttggca ttaactgttc
    12061 tgatggcgca aatgggctgt ttcgttccgg caaaacaagc agttttacct atttttgatc
    12121 ggatttttac ccgaattggt gcaagtgacg acttaattgc tggacaaagt acttttatgg
    12181 tagagatgat ggaagccaat caagccctgc gttatgctac gccaaacagt ttgattttgt
    12241 ttgacgaact agggcgagga acagcaacct atgacggaat ggcgttggcc caagcaatca
    12301 tcgaatatat ccatcgtcat gtacaagcaa aaacactctt ttctactcat tatcatgaac
    12361 tgacggtact tgagaaagaa cttccgcagc ttaaaaatgt ccatgttgga gccgttgaaa
    12421 aagacggaga agttgtcttc ttgcataaaa tgatggatgg acctgcggat aaaagctacg
    12481 gcatacatgt ggcaaagatc gcaggattgc cttccaatct actagaacga gcagcagata
    12541 tcctgcatac gctagaaaat ggcgataacg gacaccatac tgttccagct gaagtagaag
    12601 agaaaactaa aacagccaaa caggagcaaa taaaaccacc tgcagaaaaa gtaaatcaag
    12661 accatataca agaccgtgta aaagaagaaa accaacagct ttctttattc agtgaattat
    12721 ccgaaaatga aacagaagtc attgaatcac tgaagcaaat aaatttacta gaaatgacgc
    12781 caatggatgc actaaacaca ttatatgaat tacaaaaaca actgtaggaa gaaggcggat
    12841 atcgatggcc aaaatccaag aattatctga acgtttagct aatcagatcg ctgcgggaga
    12901 agtagtggag cgaccagctt ccgttgtgaa agaattagtg gaaaatgcaa ttgacgccgg
    12961 cagtactcag attgatatct tagttgaaga agcgggactg cggacgatcc aagtgattga
    13021 taacggagaa ggcattttgg cagacgacgt agagaatgcc ttcaagcggc atgcgacaag
    13081 taagatccat aatcgcgacg atctttttcg catacgaacg ctgggctttc gaggggaagc
    13141 tttgccaagt attgcctctg tttctgaaat gactctggaa acggcgaatc aggaagaaaa
    13201 acaaggcacc tatctgtcgc tgaaaggcgg agaagttatt gagcatcgac cagcaccact
    13261 gcgtcaagga acgaagatta ccgtcagcaa tctgtttttc aatacgccag cccgcttgaa
    13321 atacgtgaaa acactgcaca cagaattagc gaacatcggg gacatcgtca atcgactggc
    13381 attgagtcat ccatcgattg cttttcgttt agtacatgat ggaaacaaaa tgctggcaac
    13441 tgctggaaat ggcgatctga aacaaacaat tgcgggaatc tacggattat ctaccgcaaa
    13501 aaaaatgctg aaaatcgaag gaaaagatct agattttgag ttatttggct atgtttcatt
    13561 gcctgaggtg actcgtgcca gtaggaatta cttatcgacg atcatcaatg gacgttttat
    13621 caagaacttt tccttgaata aagcaatcgt tgcaggttat gggtcaaaat taatggtcgg
    13681 tcgtttccct attgctgtat tggaaatcaa aatggacccg cttttagtag atgtaaatgt
    13741 tcatccgacg aagcaggaag tccggctgtc gaaggaaaaa gaactaacag agctaattag
    13801 taacgccatt caaaaagcat tgtctcaaga aaatctgatt ccaagtgcag cggataatct
    13861 acggtttaag aaaaaagtac ctgatcagcc taaaacagaa caaatcgaaa tcgatttgtc
    13921 ctacgctttt gaagaagaaa aacctaaaaa gccaagtagt ttgaattttg atcgagagac
    13981 aggtaccttt ttcgtagaag aactaaagaa cacatttgtt gacagaattg ttgataagtc
    14041 tgtggaaaat gttgaaaact attctggttt ttctgaagaa aacaccactt atccagaaaa
    14101 aacagcgaaa acggtggata agtttggtga aaatgttgat aactttgtag aaaaaacagt
    14161 ggaaaaatct gaaaagaaag aatttcccat tgtggataac atggagaaaa caaatgaaga
    14221 acaagcagat aaatccgtag aagaggatga aaagatttac gaggaagaat tgacactcca
    14281 tcctgaattt gatttcactc aaggaaacga caaaacatta gaacaagcca tcaagaaatt
    14341 agaaacggaa aaaccagtac aacgttttcc tcagttggaa tattttggac agatgcacgg
    14401 aacgtatctt tttgcccaaa gtagcgatgg gttgtacatc attgatcagc atgctgccca
    14461 agagcgcatt aagtatgaat atttccggga aaaaattggg gaagtatctg atgatctgca
    14521 agaattgctt gttccatttg tgttagacta tccaaacagt gatgctttga aattaaaaga
    14581 gcaaaaacac ttgctagaag atgcagggat ttatttagaa gaatttggtc aaaacagctt
    14641 tattgtacgg gcccatccta cttggtatcc caatggagaa gaagaatcga ttattcggga
    14701 gatgatcgat atgctgctga caactggaag tgtgagtgta aaaaaattcc gtgaagcaac
    14761 agcaatcatg atgagttgca aacgatcgat taaagccaac cattatttga atgaagcaca
    14821 agcacgtgtg ctgctgaagg atctggcacg atgcgaaaat cctttcaatt gtccccatgg
    14881 ccgtccagta ttgattcact ttaccaattc ggatatggaa aggatgttca aacgaatcca
    14941 agacccgcat taagtcaaag agaagaggga aaacatgaag attgtattag catcccaatc
    15001 accaagaaga aaagagttat tggctcatat cgtttcagaa ttcgagattg aacctgctga
    15061 tatcgatgaa acgccatttc cagacgaaca gcccgtcgat tatgttcgta gaatggctga
    15121 agaaaaagca cgaactgtct gggaacaaga tgaaaaagaa gaccatttag tgattgccag
    15181 tgacacgact gttgttctca atcaagagat catgggtaaa ccagaagatc tggctgaggc
    15241 tgaattcatg ctgaaaaaac taagcgggga aacacatact gtatatacag ctgttgtttt
    15301 aaaaacaaag gaacgagaag aacggatact agcagaagca catgtcactt tttatccttt
    15361 gacagacgaa gagatcaaac gctacttgga gacgggggat tatgcggata aggcaggggc
    15421 atacggcatc caaaatgctg cgagtgtatt cgtcaaagaa atttctggag attattacag
    15481 tatcgtagga tttccgattg gtgctgtcaa tcaggcactg aaaacattca tttaaacaaa
    15541 aaagcggact atctgatttt cgattgaaaa tccatgatag cccgtttttt ttattgaaaa
    15601 gattacttat ctactctgac taagatttta cttcagcttt accgactgct tcgcccgggc
    15661 gaacaagtcc tgattggtca agagaaagtc ctgcaacttt ttcattattg gtcaaagcaa
    15721 caataatgtc agttttcttg ccagcttgtt ctatttctgg caaatccatt tcagcaatca
    15781 gggtctctgg tgtgacaagg tatccttctt tcacgaatac attgaaagga acacccttta
    15841 gttcaacagt atctaatccc atgtgaacaa gtacttccag cccctcatta gaaagaatac
    15901 caatggcatg ttttgtttca aagacactcg tcactttacc gtttactggt gcatagactt
    15961 tgccgtttga cggttcgaca gcataaccgt cacccatcat tttttgtgag aaaactgggt
    16021 catttacttc agttatttcc ttgatggtcc ctgcagcgac tgcgaataat gtttcttttg
    16081 ttgcaactgc cgtaccagtt gctgaaacag gttcgaaaga ctgtggcgtt gctgttgcgc
    16141 catttgctgt ttgaagttgt acatcagcag cattttcagg aacagcagga tcaatcttat
    16201 tcaaaattcc ctgacgtcta aagatgatcg tcaagccaaa tggaatgatg acagcaatga
    16261 tcattgcgat gatgaaaggt acccatgttt ctgcttggat agctagtatt cctggcaaac
    16321 cgcctactcc gatcgcgtta gctcgaacac ccatcaagtt agcaaacatg ccagctaatc
    16381 cagagccgac cattgcagcg acgaaaggat agacgtattt caaattgatt ccgaacatag
    16441 caggttcagt tacgcctaag taacaagaaa tcattgctgg aatcgagatt tgttcttctt
    16501 ttttattccc acgatgtaag aagatgatgg ctaagactgc agagccttgg gcgatatttg
    16561 ataaagcaat catcggccat aaattcgttg atccgaaatc tgcaatcagc tgcatatcga
    16621 tggcattggt catatggtgg agaccagtaa tcactaaagg agcatacagg aatccgaata
    16681 cggcactaaa caaccagctg atagaagacg tcaatccagt atttacgata tttgagatcc
    16741 agcttccgac tgtccagccg ataggaccta aaataacgtg tgcagctaaa acagttggta
    16801 acaatgaaaa caatggaaca aaaatcatcg agatcgactg agggatatat ttgcggaaga
    16861 agatttctag atacgctaac ataaaccctg ccagcatggc tggaataact tgcgcttgat
    16921 aaccaatcat ctggacttga gcaaaaccaa aatcccaaaa tggaatatct gctgcggctg
    16981 tggaagctac actataagca tttaataatt gcggagaaac taacgtaatt cctaaaacga
    17041 ttcctaatat ttgtgtagta cccatctttt ttgcaatact ccaagtgata cctactggaa
    17101 gaaaatggaa aattgcttca ccgggtaacc acaaaaaggc attgacacca ttccaaaact
    17161 tcgagtgttc aacgattgtt ccaccaagac tttcaaactg gataccttca agtacatttc
    17221 tgaatccaag gatcaatcca ccgacaatga ttgcaggaat cagtggtgta aagatttctg
    17281 ctaaaacggc aatagcacgt tgaacgggat gaaggttttg ttttgcagct gcttttcctt
    17341 gttctttgga aaccccttca actccagata ctgcaacgaa gtcgttataa aaagtagaaa
    17401 catcgtttcc tatgatgact tggaactgtc ctgcattcgt gaacatccct tttacgctag
    17461 gtatatcttc gatcgctttt tcgtccgctt ttcctggatc attcaggaca aaacgcatac
    17521 gagtcgcaca atgactaact gcagcaatat tttcttttcc gccgatatca tgtaacagct
    17581 tctcggcatc tgcttgatat tttgccatat caataaaccc ctttctctta acttgtctat
    17641 gcaagttgtt tcttcattta ctatactttt tttcggagag aattgcaagc gttttattaa
    17701 aaagtacgag agaaaatgat atcgttttaa aaagcttaaa ataagctttt cttaaaaata
    17761 ggaacattga aatacaaaaa gagctgtagt atagtgaaga aatgatggaa gaacgagtga
    17821 gggaaaaaac atgaataagt tcaatgaaat ttttttagat ttggaacaga aaattttagc
    17881 gaaagaatat cctccgcatt cgcttttacc cagtgagaac caattgatca aaatttatgg
    17941 tgtttcacga gaaacaatta gaaaagcgct gaatcttttg actacggaag gttatattca
    18001 aaaaaaacaa ggaaaaggtt cgatcgtact ggatcggaat cgcttcgact ttcctatttc
    18061 tggattgact agctataaag agctacagga gacccaacgt attccaagtg aaacgatcat
    18121 ccatacgtta gaagaaacag aagtgacaaa agcattgaac gagatcactg gctgggagat
    18181 cggtgcgcct gtttggcatt tgatacgcga aagaaaaatc aatggcgaag tagttatatt
    18241 agacaccgat tatctattaa aggaaatcgt tcctatttta actccagcac aagcacaagg
    18301 atcgatctat gaatattttg aaaatgagct ttctttgacg attgcttacg cgcaaaagga
    18361 gatcacagta gaagaagtaa cggaaatcat gaaaacagcg atggatcttc acgatgacac
    18421 ttatgcagta gtagtcagaa gcctcgttca tctggaagat acccgctgtt tcgagtattc
    18481 tgagtcggtt caccgattag ataaattccg tttcgttgag tttgcccgac gccgaaaagt
    18541 gtaaatagtt caaaagaaag caaattgttg gcatctgtcc actttggcgc tatgatagga
    18601 tcacaaaggg ggcattttta atggataaac caataagacc agaaaaagaa gaactgaaag
    18661 aaaagttatc acccatggct tatgcagtca cacaagaaaa tgcaacagaa cgaccattta
    18721 gcgggaaata tgatgatttt tacgaaaagg ggatctatgt agatatcgtc agcggtgaac
    18781 cgttattcag ctctgcagaa aaatatgatg caggttgtgg ctggccatca ttcagcaaac
    18841 caataacgaa aagacaagtg cgcgaaaaag ccgatttttc tcatggcatg catcgaatag
    18901 aagttcgaag caaagaagca gattctcatc tcggacatgt gtttaccgat ggaccagtag
    18961 atcaaggtgg tctacgttac tgcatcaatt ctgcctcact gaaattcatt ccatatgaaa
    19021 agatggatga attaggttat ggggaattca aatcacttgt agaataatgg aaatacacaa
    19081 agagcggtac aatacgggga tttactcgat cgtccgctct ttttttattc aagcgattgc
    19141 tatttgaaag aaacgctccc tagactcctt ttttcacaag gtgaaatgtg ctacaataaa
    19201 gggaacgtat gtgtgcaaaa gaaagaggac tagaatatgt atgaatatct gacaggtgtg
    19261 gttaccttta tcaaccccta ttacatagta gtagaaacca atggtatcgg ctaccagatc
    19321 gcactaggaa atccttaccg ctacagtagc aaactaaatc aaaaaataaa aatttatgtc
    19381 catcaggtaa tacgtgaaga tgcgcatctt ctgtatggat ttgattcgct agaagaaaag
    19441 caactgtttt tgcgattagt aagtgtttca ggaatcggtc cgaaaagtgc ccttgcaatc
    19501 atggcaagcg aagaccactc cggtttgatt caagcagtag aatccagtga tgtcacttat
    19561 ttgacgaaat ttccaggagt agggaaaaag acagcacaac aaatgatctt ggatctaaaa
    19621 ggaaaattcg gtgagctgag tatcgataca ccttttagtc tatttgatga agcaaacaca
    19681 aaagacgcta cagctctatc tgaagcaatg gaagctctct cggctcttgg ttacagtgat
    19741 cgagaaatca aacgtgtaga aaaacaatta aaagaagtag ataatcagac aacggatgag
    19801 tatctacgcc aagcactcaa gctaatgatg aaaaaataag gaggagacca aatgacagat
    19861 gaatacttgt tatcaccaga atctggcgaa aatgaactga gtttggaaaa atctcttcga
    19921 ccacagctgc tttctcaata tatcggacag agaaaagtca aacaagaact tcagatttac
    19981 atcgaagctg ccagaaatcg ggaagaacct ttagatcatg ttctgttgta cgggcctcct
    20041 ggattaggaa aaacgacgat ggccatggtc attgctaatg agatgaccgt aaatattcgt
    20101 acgacgagtg gccctgctat cgaacgtccg ggtgacttag tagcaatctt gaatgaactg
    20161 gaagcagggg atgtcctgtt tatcgatgag atccatcgat tacctagagt ggtagaagag
    20221 atgctttatt cagctatgga agatttctat gtagatatca tggtaggaca agggccaacc
    20281 gcacatccgg ttcatttccc tcttcctccc tttactttga ttggcgcgac gacacgggca
    20341 ggtatgcttt ctgcaccttt acgagatcga tttggcatca tttctcatat ggaatattat
    20401 gacgaacagg atctgcagga gatcgtttta cgttcttcgg atatttttca aacagagatc
    20461 attgaagaag gggcattgga aattgctcgc cgatcacgag gaacgcctag aatcgctaat
    20521 cgcttgctca aacgggttcg tgattttgcg caagtccaag gagacgggaa aatcgatcgt
    20581 cctatagcgg atcaggcact tactttgcta caggtcgatc aggctggatt ggattatgtt
    20641 gaccagaaat tgttgcgaac aatgatcgag ttatatggcg gcggaccagt agggttaagt
    20701 acgctttcag taaacatagg agaagagcgt gagacggtag aagatatgta tgaaccgtat
    20761 ctgatccaaa aaggattttt aaagcgtaca cctagagggc gaattgctac tgcttatgcc
    20821 tatgaacatt ttggttacga ctatcatata tgatttttgc ttttagattg gaggaaaatg
    20881 aatgagtgat acaacacgta cgaaacaatt gatcaaaaac gtattgattg atttgtgcca
    20941 aacgcaatct tttcgaaaaa tcagcgtcca aaaaatcgca aacgaagcaa aaatcaatcg
    21001 acaaacattt tattatcatt ttacagataa atacgatttg cttcgttggg tctattgtga
    21061 ggatgcattg cattatttag atccacaatg tctttcactt gataattggg aagagcaagc
    21121 attgaaaatg ctgaaagcaa ttgctgagaa gaaagaattt tatgcaaata ctgtgctttc
    21181 tgacccagaa attttacaaa aagagttttc tcaactgatc caaaaatcat ttatcaagat
    21241 ttttgaacaa atggatgaag aaagacaact ttctgcaaga gacaaactat tttatgctcg
    21301 ttttttttct tacggttgca gtggtgtatt agtcaattgg atcacgaatg aatatgaaga
    21361 aacaccgatg gaaatcgcta tgcagctttt ccgtttagcc aaggatacgg aattatattc
    21421 ttatcgtctt tatgcaaaag aagagttgga agaacagtaa tagaaaaatt ttttgttgga
    21481 aaagcaataa tcgctaaaag gagatgggga aatgacaaaa attttatttg tttgtttggg
    21541 aaatatttgc cgttcaccga tggcagaagg cttgctgaga aaacgaatag cattagaagg
    21601 aagggaagca gaattttttg tggattctgc tgctacaagc acgtatgaag ttggaaagac
    21661 accgcaccct ggaacgaaaa aaatattaaa ccaagaaaaa gtagatatga cgaatatggt
    21721 tgctcggcaa atcacacccc acgattttga aacttttgat tggatcattg gcatggatca
    21781 agaaaatgta gaagaactga aacgaagagc accacgctca gcgcagggga aaatccattt
    21841 gtttttaagt agcattcctg gaaaagaaaa agaaaatgtt cccgacccat actataccaa
    21901 taactttgat gagacatatc acttgatcac agaaggcctg aatcattggt ggaatatttg
    21961 gattgatgaa aataaaataa caaacaattt gtaaagttaa cgctttcttt ttaaggcgat
    22021 gttagagcat ttccttgttt ttttgtaaaa tgttaggtaa aataaaatta attaagtaac
    22081 actttcttag ctaaaggagg agtgcattcg atgaacgaag agacagtatc gccaatgaag
    22141 aagcttacga agttagttac tcatacttcg tatcaacttt ttgattcttt tgtaaatgac
    22201 actgaagaac atgctaagaa tgtacgattg caaggaaagt ttattcttga gctgcaaaaa
    22261 gctgctactg aacatagtct agttatctta caagtaagag caaatcgttt gagtagcaag
    22321 tatgaaacga tctcaggctg ggtggcgaca aaaacggtcg aaaaagaccg agtgatgatc
    22381 cgcctacaaa acgaagagca gcagatccgg atggttcctg tagaacaaat tgaaaaaata
    22441 acaatccttt caccagcagg ggaacaggaa cggttatcaa aataatttat accagataga
    22501 aaagcacgta tgagcttgta ccatcaatca ttactaatca aaaataaccg aatgctttaa
    22561 gctgatatca gtaaaacgga acgttttagg atgagtatca cagcatgaca gcattgggtt
    22621 gattagttga atcgattgga tggtacaagt ttttttgata aaaaaaggtc ctgctagccg
    22681 gggagctagc aggataagga gttaaaaatg aaaaagtgtt gttagggttg tttgttggta
    22741 tgcttatata atagtcacta gttgtgaata ttttgtgacg aaagttttcg aaagttgtta
    22801 agttt
//
LOCUS       DANKBF010000045        22423 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000045 DANKBF010000000
VERSION     DANKBF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            6..305
                     /locus_tag="PWY89_001840"
     CDS             6..305
                     /locus_tag="PWY89_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2066877.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PWY89_001841"
     CDS             378..1739
                     /locus_tag="PWY89_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL2066878.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PWY89_001842"
     CDS             1761..2021
                     /locus_tag="PWY89_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066879.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PWY89_001843"
     CDS             2035..2787
                     /locus_tag="PWY89_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL2066880.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PWY89_001844"
     CDS             complement(2881..3486)
                     /locus_tag="PWY89_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2066881.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PWY89_001845"
     CDS             complement(3539..4513)
                     /locus_tag="PWY89_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL2066882.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PWY89_001846"
     CDS             complement(4891..5610)
                     /locus_tag="PWY89_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2066883.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWY89_001847"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWY89_001847"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2066884.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PWY89_001848"
     CDS             7161..8558
                     /locus_tag="PWY89_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2066885.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PWY89_001849"
     CDS             8639..9079
                     /locus_tag="PWY89_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2066886.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PWY89_001850"
     CDS             9227..9553
                     /locus_tag="PWY89_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2066887.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PWY89_001851"
     CDS             9554..10303
                     /locus_tag="PWY89_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066888.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PWY89_001852"
     CDS             complement(10383..10838)
                     /locus_tag="PWY89_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2066889.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="PWY89_001853"
     CDS             complement(11265..11540)
                     /locus_tag="PWY89_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066890.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="PWY89_001854"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="PWY89_001854"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2066891.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PWY89_001855"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PWY89_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2066892.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="PWY89_001856"
     CDS             14770..17436
                     /locus_tag="PWY89_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2066893.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="PWY89_001857"
     CDS             17800..18552
                     /locus_tag="PWY89_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2066894.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="PWY89_001858"
     CDS             complement(18574..19728)
                     /locus_tag="PWY89_001858"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL2066895.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="PWY89_001859"
     CDS             19913..22354
                     /locus_tag="PWY89_001859"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL2066896.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7198 a         3933 c         4673 g         6619 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttt
//
LOCUS       DANKBF010000046        22301 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000046 DANKBF010000000
VERSION     DANKBF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            5..403
                     /locus_tag="PWY89_001860"
     CDS             5..403
                     /locus_tag="PWY89_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2066897.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="PWY89_001861"
     CDS             387..1082
                     /locus_tag="PWY89_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDL2066898.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="PWY89_001862"
     CDS             complement(1676..2563)
                     /locus_tag="PWY89_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2066899.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="PWY89_001863"
     CDS             2748..3587
                     /locus_tag="PWY89_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2066900.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="PWY89_001864"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="PWY89_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL2066901.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="PWY89_001865"
     CDS             5055..5411
                     /locus_tag="PWY89_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066902.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5414..6571
                     /locus_tag="PWY89_001866"
     CDS             5414..6571
                     /locus_tag="PWY89_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066903.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="PWY89_001867"
     CDS             complement(6597..7037)
                     /locus_tag="PWY89_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2066904.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="PWY89_001868"
     CDS             7197..7703
                     /locus_tag="PWY89_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2066905.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9784
                     /locus_tag="PWY89_001869"
     CDS             7871..9784
                     /locus_tag="PWY89_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2066906.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9789..10790
                     /locus_tag="PWY89_001870"
     CDS             9789..10790
                     /locus_tag="PWY89_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2066907.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10992..11198)
                     /locus_tag="PWY89_001871"
     CDS             complement(10992..11198)
                     /locus_tag="PWY89_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL2066908.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11844..12692)
                     /locus_tag="PWY89_001872"
     CDS             complement(11844..12692)
                     /locus_tag="PWY89_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2066909.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12691..13758
                     /gene="gnd"
                     /locus_tag="PWY89_001873"
     CDS             12691..13758
                     /gene="gnd"
                     /locus_tag="PWY89_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL2066910.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13760..14761
                     /locus_tag="PWY89_001874"
     CDS             13760..14761
                     /locus_tag="PWY89_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2066911.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14758..15042
                     /locus_tag="PWY89_001875"
     CDS             14758..15042
                     /locus_tag="PWY89_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL2066912.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15075..15872
                     /locus_tag="PWY89_001876"
     CDS             15075..15872
                     /locus_tag="PWY89_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2066913.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15886..16719
                     /locus_tag="PWY89_001877"
     CDS             15886..16719
                     /locus_tag="PWY89_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2066914.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16737..17594
                     /locus_tag="PWY89_001878"
     CDS             16737..17594
                     /locus_tag="PWY89_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2066915.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17616..18125
                     /locus_tag="PWY89_001879"
     CDS             17616..18125
                     /locus_tag="PWY89_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2066916.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18162..18563
                     /locus_tag="PWY89_001880"
     CDS             18162..18563
                     /locus_tag="PWY89_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066917.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18675..19028
                     /locus_tag="PWY89_001881"
     CDS             18675..19028
                     /locus_tag="PWY89_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066918.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19062..19505
                     /locus_tag="PWY89_001882"
     CDS             19062..19505
                     /locus_tag="PWY89_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2066919.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19522..20607
                     /gene="uxuA"
                     /locus_tag="PWY89_001883"
     CDS             19522..20607
                     /gene="uxuA"
                     /locus_tag="PWY89_001883"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2066920.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20600..21313
                     /locus_tag="PWY89_001884"
     CDS             20600..21313
                     /locus_tag="PWY89_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2066921.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21520..21669)
                     /locus_tag="PWY89_001885"
     CDS             complement(21520..21669)
                     /locus_tag="PWY89_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066922.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22065..22265
                     /locus_tag="PWY89_001886"
     CDS             22065..22265
                     /locus_tag="PWY89_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2066923.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7683 a         3427 c         4620 g         6571 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta
     9721 ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg
     9781 gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta
     9841 ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta
     9901 gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc
     9961 aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata
    10021 ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga
    10081 tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc
    10141 tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa
    10201 aacatcctac accagttatc caattgaacg aagccattca agcagctaaa aaagagataa
    10261 aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta
    10321 gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc
    10381 cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt
    10441 ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg
    10501 cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca
    10561 gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta
    10621 cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag
    10681 tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata
    10741 aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga
    10801 tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac
    10861 aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg
    10921 gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta
    10981 aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct
    11041 gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat
    11101 attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca
    11161 ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata
    11221 attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa
    11281 aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc
    11341 aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa
    11401 tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct
    11461 aaactttttt attttacata acaatcatat caactgtgaa ataaaattac aaatgataac
    11521 ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt
    11581 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
    11641 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
    11701 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
    11761 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
    11821 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
    11881 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
    11941 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
    12001 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
    12061 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
    12121 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
    12181 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
    12241 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
    12301 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
    12361 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
    12421 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
    12481 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
    12541 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
    12601 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
    12661 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
    12721 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
    12781 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
    12841 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
    12901 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
    12961 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
    13021 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
    13081 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
    13141 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
    13201 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
    13261 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
    13321 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
    13381 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
    13441 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
    13501 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
    13561 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
    13621 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
    13681 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
    13741 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
    13801 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
    13861 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
    13921 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
    13981 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
    14041 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
    14101 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
    14161 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
    14221 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
    14281 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
    14341 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
    14401 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
    14461 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
    14521 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
    14581 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
    14641 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
    14701 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
    14761 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
    14821 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
    14881 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
    14941 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
    15001 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
    15061 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
    15121 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
    15181 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
    15241 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
    15301 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
    15361 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
    15421 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
    15481 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg
    15541 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
    15601 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
    15661 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
    15721 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
    15781 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
    15841 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
    15901 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
    15961 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
    16021 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
    16081 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
    16141 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
    16201 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
    16261 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
    16321 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
    16381 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
    16441 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
    16501 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
    16561 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
    16621 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
    16681 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
    16741 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
    16801 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
    16861 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
    16921 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
    16981 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
    17041 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
    17101 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
    17161 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
    17221 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
    17281 gccgtaatgc tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat
    17341 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
    17401 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
    17461 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
    17521 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
    17581 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
    17641 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
    17701 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
    17761 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
    17821 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
    17881 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
    17941 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
    18001 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
    18061 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
    18121 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
    18181 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
    18241 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
    18301 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
    18361 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
    18421 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
    18481 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
    18541 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
    18601 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
    18661 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
    18721 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
    18781 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
    18841 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
    18901 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
    18961 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
    19021 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
    19081 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
    19141 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
    19201 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
    19261 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
    19321 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
    19381 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
    19441 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
    19501 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
    19561 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
    19621 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
    19681 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
    19741 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
    19801 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
    19861 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
    19921 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
    19981 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
    20041 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
    20101 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
    20161 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
    20221 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
    20281 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
    20341 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
    20401 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
    20461 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
    20521 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
    20581 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
    20641 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
    20701 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
    20761 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
    20821 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
    20881 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
    20941 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
    21001 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
    21061 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
    21121 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
    21181 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
    21241 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
    21301 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
    21361 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
    21421 gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa
    21481 gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc
    21541 ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa
    21601 aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata
    21661 gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat
    21721 aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt
    21781 aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg
    21841 gttattcaac tttttcttcg attcgaagac aatagaagac aatgaaattt atatttcatt
    21901 gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa
    21961 cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg
    22021 caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag
    22081 taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg
    22141 tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag
    22201 cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag
    22261 cttaataaaa attgactttt aaccgtcttt tcgaaagaaa a
//
LOCUS       DANKBF010000047        21956 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000047 DANKBF010000000
VERSION     DANKBF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            144..386
                     /gene="yaaA"
                     /locus_tag="PWY89_001887"
     CDS             144..386
                     /gene="yaaA"
                     /locus_tag="PWY89_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL2066924.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            373..1497
                     /gene="recF"
                     /locus_tag="PWY89_001888"
     CDS             373..1497
                     /gene="recF"
                     /locus_tag="PWY89_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2066925.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1494..3440
                     /gene="gyrB"
                     /locus_tag="PWY89_001889"
     CDS             1494..3440
                     /gene="gyrB"
                     /locus_tag="PWY89_001889"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL2066926.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3460..5931
                     /gene="gyrA"
                     /locus_tag="PWY89_001890"
     CDS             3460..5931
                     /gene="gyrA"
                     /locus_tag="PWY89_001890"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2066927.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6167..6466
                     /locus_tag="PWY89_001891"
     CDS             6167..6466
                     /locus_tag="PWY89_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2066928.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6516..7028
                     /gene="ssb"
                     /locus_tag="PWY89_001892"
     CDS             6516..7028
                     /gene="ssb"
                     /locus_tag="PWY89_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2066929.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7054..7290
                     /locus_tag="PWY89_001893"
     CDS             7054..7290
                     /locus_tag="PWY89_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2066930.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7433..9406
                     /locus_tag="PWY89_001894"
     CDS             7433..9406
                     /locus_tag="PWY89_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL2066931.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9412..9864
                     /gene="rplI"
                     /locus_tag="PWY89_001895"
     CDS             9412..9864
                     /gene="rplI"
                     /locus_tag="PWY89_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2066932.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10138..11505
                     /gene="dnaB"
                     /locus_tag="PWY89_001896"
     CDS             10138..11505
                     /gene="dnaB"
                     /locus_tag="PWY89_001896"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2066933.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12285..13577
                     /locus_tag="PWY89_001897"
     CDS             12285..13577
                     /locus_tag="PWY89_001897"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2066934.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14364..15227
                     /locus_tag="PWY89_001898"
     CDS             14364..15227
                     /locus_tag="PWY89_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2066935.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15356..16978)
                     /locus_tag="PWY89_001899"
     CDS             complement(15356..16978)
                     /locus_tag="PWY89_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2066936.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17227..17748
                     /locus_tag="PWY89_001900"
     CDS             17227..17748
                     /locus_tag="PWY89_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2066937.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17934..19664)
                     /gene="spxB"
                     /locus_tag="PWY89_001901"
     CDS             complement(17934..19664)
                     /gene="spxB"
                     /locus_tag="PWY89_001901"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL2066938.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19829..20647
                     /locus_tag="PWY89_001902"
     CDS             19829..20647
                     /locus_tag="PWY89_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2066939.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20750..20947)
                     /locus_tag="PWY89_001903"
     CDS             complement(20750..20947)
                     /locus_tag="PWY89_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066940.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            21116..21694
                     /locus_tag="PWY89_001904"
     CDS             21116..21694
                     /locus_tag="PWY89_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2066941.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            21789..21926
                     /locus_tag="PWY89_001905"
     CDS             21789..21926
                     /locus_tag="PWY89_001905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066942.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         7028 a         3979 c         4639 g         6310 t
ORIGIN      
        1 aaggctttct gtttattttt gtttaaatca ccttttttag attaaatcgt cttatatgga
       61 aaaatatatg gataaatttg ttttattgcg aaaaaaaagg tataatagag tatactttta
      121 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
      181 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
      241 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
      301 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
      361 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
      421 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
      481 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
      541 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
      601 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
      661 aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
      721 gcaccagaag atctttcttt agtaaaaggc agcccgcaga tcaggcggaa gtttttagat
      781 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
      841 ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
      901 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
      961 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
     1021 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
     1081 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
     1141 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
     1201 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
     1261 ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat
     1321 gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga
     1381 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
     1441 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
     1501 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
     1561 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
     1621 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
     1681 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
     1741 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
     1801 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
     1861 ttcacggagt aggatcttct gttgtaaacg cgctttctac acagcttgat gtcaaagtat
     1921 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
     1981 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
     2041 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
     2101 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
     2161 tcaatgaata ccattatgac ggcgggatca aaagttatgt cgaatacttg aatgcaaaca
     2221 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
     2281 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
     2341 tccatacgta tgaaggtgga acacatgaat caggattcaa gactgcttta actcgtgtga
     2401 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
     2461 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
     2521 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
     2581 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgg caaattgttg
     2641 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
     2701 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
     2761 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
     2821 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
     2881 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
     2941 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
     3001 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
     3061 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
     3121 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
     3181 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
     3241 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
     3301 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
     3361 tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg
     3421 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
     3481 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
     3541 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
     3601 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
     3661 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacat tgcgatctat
     3721 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
     3781 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
     3841 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
     3901 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
     3961 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
     4021 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
     4081 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatggggaaa
     4141 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
     4201 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
     4261 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
     4321 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
     4381 cgtcgtgacg ccagcgcatc cgttatcttg aataacttat ataaattgac atccttgcaa
     4441 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
     4501 aaacaaatcc ttgaaaacta tatcgaacat caacgcgaag tcatcacacg acggacggtc
     4561 tttgaaaaag aaaaagcaga agcaagagca cacatcttag aaggtttgcg tattgctttg
     4621 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa
     4681 gcaacaatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
     4741 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
     4801 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
     4861 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
     4921 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
     4981 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
     5041 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
     5101 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
     5161 cgggcaaaag gatacgaaat tccagaatac gggcgtacag ccaaaggaat cccaatcatc
     5221 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
     5281 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
     5341 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
     5401 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
     5461 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
     5521 ggggtcagag ggatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
     5581 gaaaatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
     5641 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
     5701 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
     5761 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
     5821 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
     5881 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata accatcaaga
     5941 ctaagtgtgt aagctagcaa gaataaccga ttaaaaaatc tggttattcc tgctggcttt
     6001 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
     6061 caaaatagag tgtataatta gagcttgtga gtagtctaat taattagata actctccttg
     6121 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
     6181 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
     6241 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
     6301 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
     6361 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
     6421 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
     6481 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
     6541 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
     6601 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
     6661 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
     6721 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
     6781 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
     6841 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
     6901 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
     6961 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
     7021 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
     7081 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
     7141 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
     7201 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
     7261 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
     7321 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
     7381 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
     7441 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
     7501 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
     7561 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
     7621 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
     7681 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
     7741 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
     7801 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
     7861 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
     7921 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
     7981 tgccattgat aaattagatg acaaagagat ttcttatttg aactctttcg tcactacact
     8041 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
     8101 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
     8161 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
     8221 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
     8281 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
     8341 tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
     8401 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
     8461 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
     8521 caaccagaaa aaagcttaca tcgttatcaa tgagaacgag atcattccag atgtagaacg
     8581 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
     8641 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
     8701 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
     8761 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatattgaat cttcagcttc
     8821 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
     8881 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
     8941 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
     9001 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
     9061 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
     9121 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
     9181 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
     9241 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
     9301 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
     9361 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
     9421 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
     9481 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
     9541 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
     9601 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
     9661 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
     9721 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
     9781 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
     9841 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
     9901 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
     9961 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
    10021 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
    10081 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    10141 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    10201 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    10261 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    10321 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    10381 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    10441 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    10501 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    10561 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    10621 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    10681 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    10741 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    10801 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    10861 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    10921 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    10981 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    11041 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    11101 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    11161 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    11221 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    11281 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    11341 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    11401 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    11461 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    11521 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    11581 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    11641 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    11701 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    11761 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    11821 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    11881 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    11941 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    12001 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa
    12061 atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc
    12121 acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag
    12181 cgtttttata aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt
    12241 gatacaatgg ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag
    12301 tagtaggtac gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa
    12361 atgctgaagt gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta
    12421 acggtgtgac ttataaactg catttgattc cttctgggat tttttataaa gacaagatca
    12481 gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt
    12541 tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct
    12601 tgccttatca tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg
    12661 gtacaacgat taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc
    12721 gtattgcaga cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg
    12781 acaaaaaccg tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt
    12841 ttgaagaata ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta
    12901 tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa
    12961 tgctggatat cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg
    13021 gggtaacaat cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca
    13081 aagcctatac gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag
    13141 gagaacaaat ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg
    13201 taggctggtt tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc
    13261 tatctttaaa ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt
    13321 atgaattaga tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct
    13381 gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac
    13441 ttgcagaact gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg
    13501 tacgtatctc aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg
    13561 tctgggcgca aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga
    13621 gtcgaagtgt ttatctatac gataataata acttgaagag gtagtgaaag agacgtttag
    13681 ccttattgcc gataggctgt ctcttgaaca ctgtttattc agaataaggg actgtgacaa
    13741 aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc atctacggca
    13801 ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt
    13861 gatgcagagc agctttttca caacctctcc actaaacggt gttctcctgc caactcctcg
    13921 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga
    13981 ctcggagcta aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct
    14041 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt ttttctctaa
    14101 tactcaggtc aaaacgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc
    14161 tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
    14221 atttcttgaa gctgaccact tctgtcacaa cctcttattt tgattagaac ttgcgaaaat
    14281 aaaccggtta aagtgtgtct agcgcaagag atttgctata atagacgata agggtaaaat
    14341 gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac agattcttgt tgtgatctgc
    14401 cataccatgt attagcaaaa gaaaaagttg attttttatc aatgaatctc caattgaatg
    14461 atcaagagtt agtagatgac ctcggaaaga cattcaatta tgaccgctac atgaatcagc
    14521 tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt tggccgatat ttagaatttt
    14581 tccgttctta tgcggaaaaa cagattcctg ttttatatgt ggctttttcc agtggattaa
    14641 gcggctctta cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc
    14701 ctgagatcta tgtttttgat acaaaagctg caagtattgg ggaagggctt ttagtattgg
    14761 aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa
    14821 gtaattattt gaaagtccat tcatgggtaa cagtagacga tctgaaatat ctagaacgag
    14881 gcggtaggat ttccaaagca agtgctactc ttggcagtct attgagtgta aagcctatta
    14941 ttacggtgga taaagaagga aggctgacga atgttgacaa agttcgagga agaaacaagt
    15001 cgatccagac aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg
    15061 tttatgtggc ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg cttttggaag
    15121 agcagataaa gatgaaagat gtgaagcttt atcccttagg accaacgatc gccagtcaca
    15181 caggctatgg ctgtatcgcg attttttcga tgggaatcag tcgataaaag cagtaactca
    15241 ttctgtaaaa aagcccagaa attgattgta acaaaaacag cgactcttaa aagatagatg
    15301 agcatcctat ctcttagagg tcgctgtttt tttatttttt aagctaaata ggccattatg
    15361 cccataactc gttcaattgt ttttgaatat ctgcattttc caaaaattca tcatatgtcg
    15421 tatcagctcg atcgatcaca cctttatccg aaacggcaat gatgcggttt gccaatgttt
    15481 ggataaattg atggtcatgt gacgcaaata agattgagcc agtaaatgcc atcaagccgt
    15541 cattcaatgc tgtgattgat tctaagtcta aatggttcgt tggatcatct aatacaagta
    15601 cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg gactttctct ccaccagata
    15661 aaacattcac tggtttcaat acttcttcac cagaaaacaa cattcttcct aagaagcttc
    15721 ttaagaaggt gttatcatct tcttctttac ttgcatattg acgcaaccag tctaaaatcg
    15781 ttagatcagt atcgaattct ttcgttgtat cctttggtaa ataagaatta cttgttgtca
    15841 caccccaacg tactgttcca gtatctggcg taatttcacc catgataact ttgaatagcg
    15901 ttgttgttgt tatatcattt tctgcgacga aagcgacctt gtcttctttg ctcaaattaa
    15961 aagatatatc atctaaaatt ttctttccat caatcgttac acttacattt tctacctgca
    16021 ataagtcgtt tccaatctca cgttcaggtt tgaatccaac gaatggataa cgtctagaag
    16081 aaggttggat atcgtctaaa gtaatttttt caagcatttt tttacgtgaa gttgcttgtt
    16141 ttgattttga tgcattcgca ctaaagcggg caataaattc ttggagttct ttgatttgtt
    16201 cttctttttt ggcatttgcg tttgcttgaa gtttagccgc tagttgacta gattccaacc
    16261 aaaaatcata atttcctaca taaagtttga ttttaccaaa atctagatcc gccatatgtg
    16321 tacatacttt gttcaaaaag tgacggtcat gggatacaac gatcacagta ttttcaaaat
    16381 tgatcaaaaa ttcttccagc caattgatcg attggatatc cagaccgttt gttggttcgt
    16441 ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc taataacact tttacctttt
    16501 gtccaccagt taattcactc atttgctgat ggtgcaattc ttcagggatg ttcagacctt
    16561 gcagtaaaac agctgcttct ggttcagctt cccatccatt aagctcagca aattctcctt
    16621 ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat
    16681 ctttttcttt cagcacttca tacagacgct tgtgtcccat gatgactgtt tcaagtacag
    16741 tatagtcttc atagtcgtaa tggttctgtt tcaatacggc catccgttca tctggtccca
    16801 tagtaacgga acctgtggaa ggctgcagat cccctgataa cactttaagg aacgttgatt
    16861 tacctgcacc atttgcacca atcaagccat agcagtttcc gggggtaaat ttaatattca
    16921 catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac atcatttaca gtaatcaatt
    16981 tttttcctca cttttatcat tattagtttt gacaagtctt atagaattat ataggggaaa
    17041 ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa
    17101 ataaaaataa aagcggagcg aaaatagcta ctataggatt tattagtttg caacagcata
    17161 atttttgacg ttatttataa gagggagtac tataaaactg taaaagaaat gaaagaagga
    17221 ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat tgattttggg aattttattt
    17281 gtacttgtct cattgttatc atttcaagac cctgtcggaa atttagttgc aattgtagta
    17341 gtctttgcga tttttgcctt tattaaaggg atatttgaac tgtttgtacg gaacagatta
    17401 aaagagctaa caggatataa aggaaagatg cccttaataa tcgggatcat cgatattcta
    17461 gttggcgtct ttttcttatt caatattggt gcaggagtcg cagcattgcc ttttgttttt
    17521 gcagtatggt ttattgctga ttcagtttta gcgttattta ctgctgactt ggcaagagga
    17581 gtaagtgaag gatattattg gtttacgatc atcgtcaata ttttaggaat tttattagga
    17641 attttcttat tgtttaatcc aatttcttct gcgttgacat taagcttttt agttggattc
    17701 tattttatgt tatttggtat cacacatatc gtctatgcat ttagataagt aaaatcaaac
    17761 aaataaaaaa gccggcttcc gattacagtt ttactgttgt aattggaagc cggctttttt
    17821 atcatggaca aatatgcacg aggtgatttt taggtgtcga gacaaattta tgcaaaaatg
    17881 tgagaagcta tatacacaaa ttttatctta atactcgaag catagcgcag ttgtcacaac
    17941 ctcttttcat ataattcatt caatactggc aaatcatgga cttcatattt ttctttgaat
    18001 gcttggattt cttcttcaga atattttctt ggatctaaat gcagatcttc tactggcaat
    18061 gggcgcttgt tattgatctt cacatcgatt actacaggac gatctgattt tgcagctgca
    18121 tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa agctatttgc cccgagcgct
    18181 tctccaactt taccgaaatc tgcatcttct aaaaatacac caaatttctt ttgttccgta
    18241 tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac attaataatt
    18301 ggaagctgat atttgacttg tgtaatgata tcttgcatga ccatagcaaa cccgccatca
    18361 ccacttaagg taaatacttg tttttctggg tagcttagtt gtgcggcaat tcctcctggc
    18421 acaccattac ccatagttgc gaaccatcca gatgtcgtat gtttttgttt accgttcata
    18481 ttcaataaac gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg
    18541 tcttcagcaa tacgattgat ttctttatag acagcctctg gacgaatcgg ttcttcctct
    18601 cgatcttcaa aagatttcaa ccaatttacc catttttctt tattttcttg atttgctagg
    18661 taccagctat cagcagggcg agcatcccct aactcgacaa gtctttgcaa agctgtgttt
    18721 gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc caaattttga cgcatcgata
    18781 tcgatttgga taaattcagc agttggatta aaaaatgcac gtccaaatgg gaagtcgcta
    18841 ccgacaaata agatcagatc ggcttctgcc aatgcttcat ttccgggttt agtagcgaca
    18901 cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa taattccttt tgctagaacg
    18961 gaagcgacaa caggaactga aaaatgttct gagaattctt tgatttgcgg aaatccgttt
    19021 cgcgttcctt gaccaacata aagaactggt ttttttgctt tttcaagata aggcaaagca
    19081 gctagcaaat ctttttcttc tggtaatatc acacttgttt tgtgagtgtg agcggtggag
    19141 aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag ggatagtcac gatagccacg
    19201 cctttatgct cataggcggc tttaatggct tcatctacta catgcggaag actttgtggt
    19261 gtcatgactg tacgattgta tacgctaaca tctgcgaaaa taggattttc atttagttcc
    19321 tggaaagagt tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc
    19381 acatggtcca tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca
    19441 gagccgaaga cagcgccgac tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg
    19501 ccgacttctt catgacgaac ttgaatgtag ttgatttcgg ttttttcttg atatagcgca
    19561 tccataattg aattgaaaga tcctccggga atgccgtaaa tatggtcgat accccagctt
    19621 tcaagaactt ttaccattgc gattcctgca ttgatttttg acatggtgtt tcactccttt
    19681 agcaatgatg atacataaat tttttgtatc tagagtatag aactttttat cgaaaatccc
    19741 aactaattcg tcttttcaca aaaatgtagt catgtttttg ggaatatgct agagtagtac
    19801 atagatagat tcaagagagg ggaatggcat ggttgaatgg ctattgagac ttgaaccttg
    19861 gcagcaagca cttgctggga cagcttttac ttattttatg acagctcttg gtgccgggtt
    19921 ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc
    19981 tggtgtcatg attgctgcaa gtttttggtc tttgttagat ccggctatta caaaagcaga
    20041 agaaaatgga gatattgctt ggctagttgt aagtattggg tttggtttag gcggtgtgtt
    20101 tttatatatg gcagataaaa cactgcccca tatgcatttt ggaccacaac atgaagcaga
    20161 aggattgcct acccatttaa aacggacgat cttgcttgtt ttttctatta cattgcataa
    20221 cattcctgaa ggtttagccg tgggggtagc attcggtgca gcagcaactg cggataatcc
    20281 gacagccgct atattagccg ctgtttcggt ggcgctaggt attggtatcc aaaacttccc
    20341 agaaggagca gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt
    20401 tgtttacgga caagcttctg gaatcgttga accaattgct ggagtgattg gagcgctact
    20461 agtaacgaaa gtggagctgc tgcttcctta tgcacttgct tttgccgcag gagcaatgat
    20521 ttatgtcgtg gtggaagaat tgatccctga agcacagcag acattgtcca gcaaacgtca
    20581 ttttgcagtt tttggcgtca tgagtggatt tatcattatg atgattttgg atgtggcatt
    20641 aggataaata aaaaaaatat atatgatata tcaaataggg ccgcgaactt atatcgcgac
    20701 cctatttttt tagtaagtta tatcataatg aagtgaatgg tatcatacat taataatagt
    20761 tttttctatg tgtatttcct ttgatccagc gaacaagcca gatgataagt gcgattggga
    20821 ttagaacttt gaccgcaaac cataaaagcc cgcttaggac actgacaaaa agactgatga
    20881 cgaagctagc tgcaattagc actaaaagaa ccatgaagat atttaagaac atattttttt
    20941 gacgcataca aaaccctcct cttgttcatt ctttactata gaaaaagtat aacagggatt
    21001 gtttaaaaac acctccaact gaagactgat ttttacttca tctacttaaa tgatcaaaca
    21061 attgcttttt ctctggttta tcatctaaaa tagagcggaa aaggaggaga atatcatgcc
    21121 taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg attatcatga ctattttagt
    21181 tttagaactt catgaaccta acggagacac gtgggaggcg ctcgctgcat taggttaccg
    21241 attttttatc tatattgtca gttttatcat tttagcgatt tattggaaca atcatcatca
    21301 tttatttcaa gttgtacata agataaatgg acgggtctta tgggcgaaca gtttctttgt
    21361 ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc agtgagtaca tcaatagcct
    21421 ggctccagaa atgacttatg gttttgttac cttgaccgct aatattgcgt attatatctt
    21481 ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa gcattgaagg cgatgttgaa
    21541 taattacaag aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg tcttagggta
    21601 tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg attctagctg cttggatcat
    21661 cccaagtcgt accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag
    21721 aaaaatatag aaaaaataaa taaacgatca aatatccgag tgatactagt attcaaagag
    21781 ggatgcagat ggatcaaaaa agaattcatg taggactgat tactagtttt gtcacgatgc
    21841 ttatttattt ggatcaaggt catttgactt ggacagtagt tggtgtcgta ggatttttga
    21901 cattattcac agcaataaaa aaataaacat ctttatgtaa tctgatgttc tatcta
//
LOCUS       DANKBF010000048        21923 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000048 DANKBF010000000
VERSION     DANKBF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            75..503
                     /locus_tag="PWY89_001906"
     CDS             75..503
                     /locus_tag="PWY89_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2066943.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="PWY89_001907"
     CDS             490..732
                     /locus_tag="PWY89_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066944.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="PWY89_001908"
     CDS             complement(1032..1247)
                     /locus_tag="PWY89_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066945.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="PWY89_001909"
     CDS             1354..1668
                     /locus_tag="PWY89_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066946.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="PWY89_001910"
     CDS             1772..2950
                     /locus_tag="PWY89_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2066947.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="PWY89_001911"
     CDS             3353..3646
                     /locus_tag="PWY89_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL2066948.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            4488..6815
                     /locus_tag="PWY89_001912"
     CDS             4488..6815
                     /locus_tag="PWY89_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2066949.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            6941..7270
                     /locus_tag="PWY89_001913"
     CDS             6941..7270
                     /locus_tag="PWY89_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2066950.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            7310..8314
                     /locus_tag="PWY89_001914"
     CDS             7310..8314
                     /locus_tag="PWY89_001914"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2066951.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            8311..8757
                     /locus_tag="PWY89_001915"
     CDS             8311..8757
                     /locus_tag="PWY89_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2066952.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            8907..9722
                     /locus_tag="PWY89_001916"
     CDS             8907..9722
                     /locus_tag="PWY89_001916"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2066953.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            9928..11100
                     /locus_tag="PWY89_001917"
     CDS             9928..11100
                     /locus_tag="PWY89_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2066954.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(11262..11756)
                     /locus_tag="PWY89_001918"
     CDS             complement(11262..11756)
                     /locus_tag="PWY89_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066955.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(11753..11947)
                     /locus_tag="PWY89_001919"
     CDS             complement(11753..11947)
                     /locus_tag="PWY89_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066956.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            12342..12764
                     /locus_tag="PWY89_001920"
     CDS             12342..12764
                     /locus_tag="PWY89_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2066957.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(12930..14123)
                     /locus_tag="PWY89_001921"
     CDS             complement(12930..14123)
                     /locus_tag="PWY89_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2066958.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            14347..14724
                     /locus_tag="PWY89_001922"
     CDS             14347..14724
                     /locus_tag="PWY89_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2066959.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            14712..15050
                     /locus_tag="PWY89_001923"
     CDS             14712..15050
                     /locus_tag="PWY89_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2066960.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            15184..15627
                     /locus_tag="PWY89_001924"
     CDS             15184..15627
                     /locus_tag="PWY89_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL2066961.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            15889..16704
                     /locus_tag="PWY89_001925"
     CDS             15889..16704
                     /locus_tag="PWY89_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2066962.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            16714..17376
                     /locus_tag="PWY89_001926"
     CDS             16714..17376
                     /locus_tag="PWY89_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2066963.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            17581..19245
                     /gene="alsS"
                     /locus_tag="PWY89_001927"
     CDS             17581..19245
                     /gene="alsS"
                     /locus_tag="PWY89_001927"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2066964.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            19258..19968
                     /gene="budA"
                     /locus_tag="PWY89_001928"
     CDS             19258..19968
                     /gene="budA"
                     /locus_tag="PWY89_001928"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL2066965.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            20098..20595
                     /locus_tag="PWY89_001929"
     CDS             20098..20595
                     /locus_tag="PWY89_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL2066966.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            20780..21040
                     /locus_tag="PWY89_001930"
     CDS             20780..21040
                     /locus_tag="PWY89_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066967.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(21402..21641)
                     /locus_tag="PWY89_001931"
     CDS             complement(21402..21641)
                     /locus_tag="PWY89_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066968.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         7450 a         3706 c         4314 g         6453 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
     4261 aagcaatact tcaattaatc aagttttatc ttttttttca ataatcgtaa aaaaagagta
     4321 ttttttattt aaaaataaaa tctttgtatt aaaggacaag actaaaataa ccttagaata
     4381 aaaagagaaa aaaagcccca aactttctat acatcaataa aataataaaa attaaattta
     4441 gaataacgtg taaaggatta tttgatgatt ttttattgag aggagggatg aaaatgatta
     4501 agaaaactca aaataaaatc caacgtatat gtcaaaaaca ttttgtttct agactcaaag
     4561 ctgtatctaa aagaaaatac ttacatccca ttatgatttc gatcatcttg atttttactt
     4621 tcttagggag cttatcaagt atttatgtaa acgtttatac agaaacgtca aattttgcta
     4681 accaaacgac agaggagaca aatagtccac cctattctga aatacagtcc atagaaagca
     4741 ccaatcagtc tagcaacaac gagacaacct caaccacatc ctcgactcaa ctgattgatc
     4801 cgacagaaaa agaaatcaca gcagcaaaag cagcagggga gaaagcttat tcagaaacag
     4861 gtagaagcca gacatttagt actgttgcgg ccactgcgaa tttcaacgga cataccatac
     4921 cagatggata tacagtgata tatgccaaag atggagtcat tgttgcggtt aacggatcga
     4981 tgagtgcgga taaaactgcg ttacaaggcg taaccaaagc aatcgctttt attgataacc
     5041 gtccagatat ccataatctg tttattggct ccacagtaaa tggtactact atcacattcc
     5101 caaaaagtat ctatatgggg gtggctaccg ctggaaatac gatcgattca gttacaccca
     5161 atgcggataa aagttattct tttacaccct caacatgggg agggcttcct gccggaggag
     5221 gtatgagtac tttttcaatc ggtatacagt tagcaaatga tattccattc acggggtact
     5281 cgatcaaaaa tgggcagatc gttgatggta agggaaatgt ttcaaatttt ccaacctatc
     5341 aagcagcaac ggttcaggtc aatgcgaatc ggacaggaac tttttcttat acagtcaatg
     5401 gtggagcagc aaaaatcgga acgtacggga ctacattatc tggaatccat gtgggtgaca
     5461 aagtggttat cactccaaca gcagtaacgg gctattcttt tgcacaaaca aacggttctt
     5521 tcacagccct agaaagtaac gtcgatacgg tgacctatac aggcaaacaa acaccaatca
     5581 ctattaagta tcaagactat tttggacaga tgattgctcc ttctaaaata atgaatctta
     5641 cttacggaag tgctgcacaa gacctgacga ctaatacgcc tactatcaac ggatatacct
     5701 ttacagctgt ttcagcgata gaaacaaaga accaatcagc aatgagtgtt ttagctagtt
     5761 tagatacaaa tggaaatgta atcgtaaagg acgcaaatgg aaaacagatt tctgaagtca
     5821 tcctatatta taagacaaat gcaacagtat caatcaatgc aaatggcagt aagtactatg
     5881 atggtctttc tgttgtaccg atcgttaaat atgcctttaa tgataaaaca gaatcaacat
     5941 cccttttaaa caatgatctc agtattgatc aaatcacatg gaatccaact gatttcaaag
     6001 cgatgaatga aaagggagtt gaagtaactg cgcctactga aattggaacc tatacgtctt
     6061 ggcaattgac aaaaagcgga ctagctaaac tagctgccag aacaaactat ttatttacta
     6121 tcgtgcagac atctgatgtc tatacgatta agcaaatcag cggtgcggta gagctcagtg
     6181 gcagtaagac atatgatgga aaagcaggtg tcccgtcaat ccatgtcaag ctagcagagg
     6241 gtgtcactag ttcgcttact ccagtacaag tggcacttag cagtatggat tacacagcag
     6301 atgcacaatc agcaaaaaat atggttaatg ctggcagcta tacaattaaa ctaacgaata
     6361 gcggaataga caaagtcaaa gcagcaaatc ccaagttgtc atttactgat atagccaaca
     6421 catcaggaac ttataccatc tataaagcta acgcagtgat tactgtcgat gacgctgcct
     6481 ttaattatga tgcccaaagt cactcgattt cggttggaaa tctccacgtt accggtgttg
     6541 tcttaggcga atcattggat tataccctaa cagttaacag ccggacagat attggatcac
     6601 aaatagtaac gattagttta ggcgaaggaa gtgtaaacaa taattatacg attatcgcaa
     6661 cagataccgc taagttgacg atcaatccga ctctcttgct tccttcgaca ggtgggatag
     6721 gtctactgcc ctttgtcttt ttaggactga tctttatttt ttctggcttc ttttatttta
     6781 tccatcgtaa gaaaaaagca ggtgaacagc gatgaaaaaa acaaaaaatg tacgtgtgga
     6841 tatatttgct gttctctttt ttatcctact ggtctttttt ataggttctc cgactgtgtc
     6901 agctagagaa tcacctatta catttacttt gcataatcga atgttcgaaa gcgataagat
     6961 gccaagtatc agtcaaaacg acggaataaa tatcccggat aactttcaag gagtaaaagg
     7021 gttgaacggc gttactttcg ctatatatga tgtctcggat gaattttaca agctgcggtc
     7081 agaaggaagt agtgtggaag acgcacaaag aaaactagca caaaaaagtg atagtgaaaa
     7141 aatactggca gaaaatgtga caaaaacagt agacgaggaa gagggcattg cttcttttag
     7201 tgcatcggac aaagatgagc aaggtcgtga cgctgtttac cgatttacgg aaataaagac
     7261 ttcagattaa gtaaaagaaa aatcagcacc atttgtcgtt gtgcttcctg tgacagattc
     7321 atctaatcat aaattgacga ccatccactt gtatccaaag agcgaacaaa aaatacctgc
     7381 tgcactcaca ttaacaaaaa cagtagagaa taagcagaca gattttgcag atggggataa
     7441 agtcccttat ctcatcaccg taacaattcc tgagaacatc aacgagatag gaacctatac
     7501 gatcaaagat acagctgatc ctcagttatg gctagaacca gaaacgatcg attttttgat
     7561 tggtggagac aagattcata catttcacac acaaaaaata aaacatggtt ttgtcttatc
     7621 agattctgga aaagatctta gttcgtttgc tggaaaaaaa ttaacgatcc gttaccaaat
     7681 gacattgaaa gaaaacagtg aaaaaacgac atttgataac gatgtacgtt tgacgactga
     7741 caatcaaaca gtagaaacac atcttgccat ccaaacagga ggcaaacaat ttgtcaaagt
     7801 ggatcggcaa acgaaacaaa aactagcaga tgctcaattt ctagtcaaaa aaaatgacca
     7861 atacttagcg caagaaaacg ggataaatca ttgggtagcc aaagatgatt caagagcaac
     7921 gatttttact tccagtaaag atggaacatt ttctgtccat ggattgtctt ttggaagtta
     7981 cgaactagtg gagattaaac cgcctaaagg atatatattg agtcaatcaa ccattctctt
     8041 tgaagttgaa aaaggcagtt atgatccgct tcatcctata ccattggaaa tcatcaacga
     8101 accaaaagca aatccgacga ataatggaaa aacgccagtt cagatgcaga caaatggtcc
     8161 atcaaatgaa aaaaatggag gaagctttcc aaaaacaagc gaagagatgc ttggaggttt
     8221 ctcaatttta gggttattgc tagtcctttc aacagggaca gcttggtttt ataaaaaaca
     8281 acaaaaagga aacaatagga gggaaataaa atgaaaaaca tcaaaacaaa aatatggacg
     8341 tttttaggaa ctgctatcat gctgcttcct ttcgttctcg gcctaggaac tgcagaggtt
     8401 tctgcagccg tatcacctac accagaaaat gttacggtta atttgcataa attgaaattc
     8461 acttcagcac cagaaaatca aataaacaac ggaacggaat tgacatttcc gaatagcgaa
     8521 ccgttgaacg gcgttgaatt caacgtatat gacatcactg ctacgtacta tccaagtaaa
     8581 gacacagcag ttccagcaga cgctacaccc ttcgcaagtg taacgacttc tggtgaagga
     8641 ttagctaacc tgactcttcc aggtaaatct gatgggaaag atgctgtata cgtctttgta
     8701 gaaacaccaa agcccggtgt cgaaacgtcg ccaaatatcg tgttaagctt accctagacc
     8761 ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa gaaagcaata
     8821 tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg gcgcaattcc
     8881 agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact ggtgtcgctc
     8941 tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga tccatcagtg
     9001 tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca gatggttatc
     9061 aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa ttgcaacaaa
     9121 caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac ggtgtagtca
     9181 atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa aactacggtg
     9241 atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat gcagaatttt
     9301 ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca aaccttgcct
     9361 caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg gaagaagtga
     9421 aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa ggggcatact
     9481 gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa gctgcgtaca
     9541 ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc gataaccaac
     9601 ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc ttggtaggta
     9661 ctgctttagt tggaggagca gtgatctatt tcaaaaaacg ccatcaagaa acagaagctt
     9721 aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa tggggagaaa
     9781 ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa taggaggaac
     9841 tattttgccg tttgcttcgc taatactcag gtcaaggcgc tgatttcaca acctcttcat
     9901 atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat actggacatc
     9961 ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt tgtagaagat
    10021 tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca agcatcgaat
    10081 aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa ccaacagttg
    10141 gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga tgcaaaagca
    10201 ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt catcgaaatc
    10261 ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt tttgcaaaaa
    10321 ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac ccatgccgtt
    10381 ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc taagttgaaa
    10441 aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca agtagaaaaa
    10501 atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg cagagatctc
    10561 gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct tgtgacgggg
    10621 catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat tgatcaatgg
    10681 aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat cggcttagtt
    10741 tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta tcctttatct
    10801 ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc tgtaaaaacg
    10861 ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc ttctgatgaa
    10921 tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca acatgaggag
    10981 ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat gaaattgaaa
    11041 aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga gaaagaatga
    11101 atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag aagctgacga
    11161 aatggacgtg taaactgtta tttttggttt actatctgga acgccatctt ctattttttt
    11221 atgtttcaga agaattttat tattatagta taacttttga attagtttcc ataacatttg
    11281 gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt atacgtacca
    11341 tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac agtttgttca
    11401 ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc tgctgattgc
    11461 tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gcctcgcctg ttctgcttgt
    11521 tgctgtgcga ttgcttcttg ttgcgcttgt tctgcttgtt gtcgtgcaat ttcttcttgc
    11581 tttttccgtt cttcttctgc ttcttttgct ttgattgtgg aatcaattgc agcaagacgg
    11641 cttgtcaact cttgtttgtt ttcaggtagc gcttggatag ctgctgaagc agttgcatag
    11701 ttttcttttg taggatttgc ttcagcttgt gacaaagcag attccgcttc tgtcactttt
    11761 tttctagtgc agctttttcc gctttttgtt tctcttcttt tttcttttca atttgttctt
    11821 gttctttcga tgaatctgcc gtactttcgg agatcgtggt actgctgctg gaagcctgat
    11881 ctgaacttgt gctttgagta gtagacgtag tagcaccaca cccactcaac aaacttcctg
    11941 cgaccaaaaa caatgcaact aattttttcg ctaaatttcc tccttaagta aaaaacaacc
    12001 gttttcaata aatgtccttt tcaattgttt acttacttgt tataataatt gtgttatgaa
    12061 tagtgattga ggaaacttaa tctaatcccg tgttgataca cgggattttt tgctatagtt
    12121 ctgttattaa gtatgcaata ataaaatatg tacgttattt tattattaga ttattgacat
    12181 ttatataata aacgattaaa tgattcatat caatagaata cttaggaaaa aaagagaaat
    12241 aataacaggt ttatgcacta ttgacggtgt gacaatatga tatttgtatc cgtttttttt
    12301 cttgtatgta gtatagtaaa gtagatacag gaggagaaaa tatgcagact tttttaacaa
    12361 aagtttctca aaagaaaatt aaaatgattc atttactgct tgaaacagaa cgttggtgtt
    12421 caatagaaga actacaaaac gaattgaagg tatcaagtaa gtctatcttg aattatctaa
    12481 cagatttaga agaattgttc caaaaatatc ctgataaggt cattttgaag aatgagaata
    12541 atcggcgctt ttctatggaa aagcaggaga acttcccaat ctacattatt tatcttcatt
    12601 tctacagaaa atcatataat tatcatttga ttgaattcat gtatcaacat cctgagaaaa
    12661 atttggaaga ttatgcagac gctcaatata cgagtgtatc cacagttttt cgttatgcta
    12721 agttattagt aaagtacttt gaaatgacgg acgtcaaaga atagtcgccg ccagatagaa
    12781 ggaaattagc agttgaaaaa gctgccctgc atcaagtaat acgaccagcc aaacggaaac
    12841 agtgtctcat gtttttgatt tggctggtct tattaccgca gatgcaagct tttgccacac
    12901 ctctttcttg tcatttacag actcaactat caatgctgca agctttccct taatgctgta
    12961 acaccagtaa gagttgtcaa gaccagcaga ctgactaggt aaataccgct caaaaacaag
    13021 attacggcag ttaaactata atgatccatc aagaaaccga tgacgataga agaaaaacca
    13081 ccaacagctc gtccaaggtt cataatgaaa ttattcgccg tcgcgcggat ttctgttgga
    13141 tacaaactac taatcactgc gccatatcca ccatacatgc cattgataaa gtagccgact
    13201 atcacagcgg ctgctaccaa actccactgg ctatgggcta acacaagtag atagacagat
    13261 aaggatgaac aaattaaaaa caaagaaaag gctagtttag gccccaattt atccatgatt
    13321 atgccaaaag tcatcatccc caaagacatg cctaaaatag tactgaccat ccacaatgaa
    13381 gacccagaaa ccgaaagccc taatcgattt tgcataattg aaggcagcca attcattaac
    13441 ccaaaatagc cggcaatttg gacagttacc atgatactaa ggccacatgt ttgccaagca
    13501 agatgttttg attcaaacag tctgctaagt ttggttttct tttcggcaga ttgtacagat
    13561 ttgaattcct ttggttcagt caagccgcga cgaatgaaca agaccatcaa caccggcagt
    13621 acacctacga catacaacat cttccaacca aacgttggaa tgatcacagc agctaaaatc
    13681 gcagccaaag ccgcaccgac ctgcgcacca ataccagcaa ccgaagaagc tcgtccaatt
    13741 tgtttttttg aaaaactttc ggaaatcaag gacatacacg ccccgtattc tcctcccgct
    13801 cctacacctg cgataaagcg aaagagataa accaagtaga tgtttgaagc aaaatacatc
    13861 aataaagaag caatcgaaaa cagaaggacc gtttgagaaa atactttcac acgtccatat
    13921 ttatcagcta tgatgccaaa aaaaatacct ccaatgagca tccccaaatt agtgatcgtt
    13981 gaaatgaaac ccgcttgcgt accactaata tgaaaagaag taatcattga agataatgaa
    14041 aaagctaaaa acataatatc catattctct aaagcaattc ccgccgatgt cgataataat
    14101 acctttcgct gatacgttgt catttttata ccccttttcc taaaatttcc ctaaattgtt
    14161 aaaagtatat ccaaaaaaga ataaaaaaac aagacttttt tatttgaaat aatacacaaa
    14221 caaccgatat agataatcca gcttagtttt aatagaaatg agtattgact tggagtacac
    14281 ttaaagttgt agaataaata aaatacataa gtaaagtggc aagaaattaa gagaaagagt
    14341 gatcagatga atatcaaaca agtcagtgaa gaaaagggca tttcggccga tacattgcgc
    14401 tattatgaac gaatcgggtt gattccacca gttaatcgga caaaaggcgg aatccgtgat
    14461 tatacagaag aagacctaaa atgggtcgac tttacgatct gtatgcgaaa tgccggatta
    14521 tcgatcgagt ctttgattga atatattcgc ctatctagcg aaggagaagc aactgtattt
    14581 gagcggcgta agcttttgat tgaagaaagt gaacttttga cgaaagaaat tgctgaaatg
    14641 caagagtgtt tggaacgttt acaaggaaaa atcaagaaat atgatcgtgt gttatctaga
    14701 aataaaatgg aatgtatcaa gtaaaaaaat caaggagcat cgctatggag ttacgcttac
    14761 taaaatattt ttggacaatt gcagaagaag gaacaatctc tcaagccgca gaagttctac
    14821 atcttacaca accaacctta agccgacaac tgaaggagtt agaagaggaa ctaggaacag
    14881 aactgtttgt acgcgaaaac cgcaaaatga tcttgacaga agcaggttat tttctaaaaa
    14941 agccgagcag aagaaatttt agatttaacg aatcaaacca ctcaagaatt cgagaacaga
    15001 aaaaaacaat tattcagcgg ccgatttacg atcggctgtg tggaagctga taattcgaat
    15061 acattggcac tattactaga aaagttgatc cgtgattatc ccagcgttca atttcatatc
    15121 cacagcggaa caagtgatga tatcacggag tgactagaaa aaagcctgtt agatttagcg
    15181 attttgctag atccagtaga tacagagaaa tacgaaacga ttttgttacc aagaaaggaa
    15241 aaatggggat tgctcatttt agaaaattct gttttagcaa aaaaagacgt gataacaaaa
    15301 gaagactttg cgaatgtacc tcttttgatc tcaaagcgtc cagaaataca gcgaatgttt
    15361 gctgactggt taggcataaa aaaggagcag ttgctgatca caggaacgta caatttgatt
    15421 ttcaatgtat ttgcgctaat tgagaatcag gtaggtgctg ctttagtcat agaaggtgct
    15481 gtaagtcatc tttcaaagcg agtgacattc gttccgcttg acccaatgat tgaaacgaac
    15541 tgcatgctag tctggaaaaa gaatcggatt ttatcacctg tagtcaatga attgattcac
    15601 cgtttcaaag aagcttttga acagtaataa gtatctctaa tcgaagaaat ttcaatacaa
    15661 aagcgagacg tatgacagtc ttgcttttgt attttttcat ttttaaatca aaaaaaataa
    15721 agagatttac ataacttgtt aaataagcgc ttacaaacat aagaagaacg gaaatgaatc
    15781 agctataata accgttgttc tggattgtaa tcattacaaa gaaatgaaga ttataacttt
    15841 ggaggtgttc gaaatgaaag aaaaaaagca aaaattcgtt caaaaaacat gccctttatt
    15901 caatggttgg gtatgattct gctgattgta ttagctttat tgacgatcat tcctttttta
    15961 tggatggttt ctgcttcatt caaagcaaat aatgaagtgt tcacgattcc gattcaatgg
    16021 atccctaaaa cttggcattt agagaattat ctggttattt ggtctcgtat tcctttagtg
    16081 acatttttta agaatacagc ttttttaagc gttgtgatca cgttgattca gttgtttact
    16141 tctagttttg ctgcttacgg gttttctaaa ctaaatttta aagggcgcga tattttattt
    16201 ttagcatata tcggcacgat tgctgtccct tggcagtctt atatgatccc gcagttcatt
    16261 atgatgagaa aagtgggttt aacatatacg ttgtggtcgt tggtgctatt acaagcgttc
    16321 aatgcttttg gtgtgttctt gttgaagcaa tactacagta gtatcccaga ttctttatgt
    16381 gagagtgcgc ggatcgatgg cttatcagag tggggaatct atcgcaagat cattttacct
    16441 cttacaaaac cggcgattgc cagtttgacg atcatcacat tcgttaatac atggaacgat
    16501 tatatggggc cattcattta tttgtcttct acggaaaata aaacaatcca gttagggttg
    16561 aaaatgttta ttgggctatt tgatgcagag tacgcattaa tcatggctgc ctcagttgtt
    16621 tctgttcttc ctgttgcttt tatattttta gcgatgcaaa aatattttgt agaagggatc
    16681 gcttcatcag gaatgaagga ataggaggtg attatgaaag agcagcgttt tgacaaaaat
    16741 gtttatatgg aattctttta ttggttgtat caatcattgg caagtagcct ttgtttttgg
    16801 tgtgtcaata tcttgtatat agcagctgcg ttccttttgt cgatagatgg aaggaatgtc
    16861 ttttccttta ttttttcttt actttttatt ggtccaggtt gcatcgtgat gataggtatg
    16921 acagaagaat tagcaaaaga aaaaagtttt tcaccagtac gtttgttcat aaagacgttt
    16981 cgccaatttt ggctaaaggg atttttttat tggctatttg cttggatagt tagtgtgata
    17041 atgatttttg attgtttctt ttttatacgt ttttcttatg gaaaatggtt gattccgttg
    17101 tttgttttac ttgcttgttt atcagtttcc tttagcatca actactggta ttttcaggtg
    17161 agaaatccag cgtcgaaacc aaatcaagtt ctacggatag cgttctacta tacgttgaaa
    17221 aaatggtatg tgagtttact agatttttta ctattgacaa gtttgtttct cttctttttc
    17281 gtaaagcccc aatggtgtat tctactaggt ccttctatcg tatttggttt gatttacttc
    17341 aacaatcgta aattgatgag aactatggat ctgtagactt tataaattga aactttcaat
    17401 taagaatgac agtttttatg taaaaactgt cattcttttt cattttttga aacatatggc
    17461 taaacaaatg aaataaaacg tgctatactt ttgaatgaaa caggtttaga aactgtatca
    17521 attatttgtg attatttcta actaataatt aaaaataaag cataaaaagg acgatgttca
    17581 atggacgaaa aaatgaaaca aggttcgaaa tctgtagtag aaagtttgat taatcatcat
    17641 gttgattatg tattcggtat accaggtgca aaaatcgatg gtgtatttaa tgaattagag
    17701 gatcaaggtc ctgaattaat cgtgactcgt cacgaacaaa atgctgcatt tatggcacaa
    17761 gcaatcggac gtattacagg agagccagga gtagttatcg caacaagtgg tcctggtgct
    17821 agtaacttag ctacaggttt ggtaacagcg acagcagaag gtgacccagt tttagcaatt
    17881 gccggacaag taaaacgaag cgatctattg aagttgaccc atcaaagcat ggataatgca
    17941 gcactgttcc agccaatcac aaaatacagt gcagaaatcc aagatccaga aacaatttca
    18001 gaagtgattg ccaatgctta tcgtatggct aagagttcaa aaaaaggcgc gagctttatc
    18061 agtatcccgc aagatgtagt ggatgcacct gtccaaggga atgtcatcaa gccattgagc
    18121 gatcctaaat taggttctgc ttctgcagat gatatccgtt acctagctga acgcattaga
    18181 gaagcaaaac taccggtact attagtaggt atgcgaggat caagtgaaaa agaaacgtta
    18241 gccatcaggc aattagttgg aaagacagca ttgcctgttg tagagacatt ccaagcagct
    18301 ggagtgatct cacgtgaatt agaagcgcat ttctttggtc gtgtgggttt atttagaaac
    18361 caaccaggag atatgctttt gaaacgaagt gatctagtta ttgctattgg ctatgatcca
    18421 atcgaatatg aagcaagaaa ctggaatgcg gaaaaagatg cgcggatcat tgtgatcgat
    18481 gaagcgccag ctgaaatcga tccttttatg cagcctgaaa gagaattgat cggtgatatc
    18541 tcagccactc ttgatttatt gacaggttca ttagagcctc aacaagtgtc agaagatgca
    18601 aaagaatatt tagcatctct tcaagcagaa ttaacggagc gggacattgt tcaatcaaaa
    18661 ggagaagcag gtatcttgca tcctttagaa gttattaaca cgcttcagtc aaaagtaaca
    18721 gatgatatga ctgttaccgt agatgtaggt agtcattata tttggatggc acgccacttt
    18781 agaagttatg aacctcgtca tttgttattc agtaatggta tgcaaacgct tggtgttgca
    18841 ttgccatggg cgatttctgc tgcattggtg cgtccaaata cacaaatcgt atcagtttcc
    18901 ggcgatgggg gatttttatt ctctgcccag gatttagaaa cggcggtacg taaaaaattg
    18961 aatatcgttc atttgatttg gaatgatgga cactacaata tggtagagtt ccaagaaaaa
    19021 atgaaatatc agcgggcgtc aggtgtagac tttggtccag ttgattttgt gaaatatgca
    19081 gaagcttttg gcgctaaagg aatccgagct acaagtgtag aggaattaga aaaagcttta
    19141 gaagaaggat ttgcgacaga aggacccgta attattgata ttccgattga ttaccgtgat
    19201 aatgagaaat tgggagaaac aatcttacca gatcaattct attaaggaag tgtcgacatg
    19261 actgaaaaaa tcttatacca gcacgggaca ttaggagctt tgatggctgg cttgatggat
    19321 ggaacagaaa cgattgctca tatattagaa aaaggaactc tgggattggg aacattgcat
    19381 gggcttgatg gtgaagtcat ctttttagat ggcgtagctt atcaaggaag atcagatggc
    19441 tcagttgccc aattagatgg aagtgaactt accccctatg cagcaattac agattttacg
    19501 ccagatacta gtttttctgt ctcaaaaact gcagatggag aacaattgaa aaaagacata
    19561 ttagtaagag aagccgggga aaacctattt ttggcagtga agataactgg attattcaaa
    19621 aatatgcaca tccgtatcat gccgaagcaa gaaaaacctt acagaagact agcaaaaatt
    19681 tcagagtctc aaccagaatt ccaacaaagc aatgtccaag gaacattagt cgggttcttt
    19741 acgcctgaat tattccaagg agttgctgca gcgggatttc acttgcattt tatcgatgat
    19801 acacatacat ttggcggaca tgtaatggat tttgaagtgg ctgaaggaaa agttgagatt
    19861 agccgcatct cgtcccttgt acagcatttt ccagttgatg atccgacttt tgttcacgaa
    19921 acgattgatt atgctgattt agcagcagaa atccaacaag cagaatagaa aaagacagcg
    19981 agatgcgttc aaggtgatta acctgacgta tctcgttttt ttgaggagga aacctagcgt
    20041 ttacaaaaaa actgagctat aatcttctcc atagatcaaa aaggagcggt ttttatcatg
    20101 tggacagaaa aacagaaaaa agatgcatac gaaattttat tattacagca acaaggatta
    20161 ttagaagcag aagataactg gttagcaaat ttagcaaatt catctgcatt gttgaatgag
    20221 actttgcctg agactgtctt tgcaggatat tatttattcg atggtaatga attgatttta
    20281 ggaccgttcc aagggcgagt ttcttgcaca cgtatcaaga tgggaaaagg agtttgtgga
    20341 gaatcagctg aaaaacaaga aacattgatc gttgatgatg tcaaaaccca tgcaaattac
    20401 atttcctgcg attcagccgc acaatcagaa atcgtagtgc caatggtaaa agaaggtaat
    20461 ttaatcggtg ttttagatat cgattgcgga gtaacgaaag ggtatgatga aatcgatcaa
    20521 gcatttctag aaaaatatgt agatctttta ttagcaaatt ttaatataaa tgctttttca
    20581 tttattaata actaaaatcg tctattgaaa caaaaaagaa gagaaatcaa gaataaaaag
    20641 agccggaaag acagtgggca tttctgtttt tcggtttttt tattttttta taaaatcaca
    20701 agtgttattg attttcataa tcatatttta atggtattat cattttaaat aatggaaaaa
    20761 cattaaggat ggatgaaata tgaataacga aactatcatt ttaggaaaaa aatatatttg
    20821 ccaacctatt ggcttgaaac aaaaagtggt tggagaagta atcagtaaat tagaaaattg
    20881 tgttgtgatc tgcgtagacg aatatcatga cgtcgatcgc aatgaaatat tagaaaaatg
    20941 cggaaaagta gtggcaaaat atactcacgt ttatgagttg gaaacaacag aatatctata
    21001 taactatagc aacaaatatg aacatacttt agtttcataa agatttaaat aaagtgcctt
    21061 caaaagcgag aagaccaagc ttttgaaggc actttattta gttggcagtg attgttcatg
    21121 atttttgatt tattcaaaag gttcgcctag agaatctgtc tctgtaacga ggcctggtgg
    21181 agaaactcga taaaaaccta tactgccaga actgtcctat tttcggatag acaaactagt
    21241 agttttaaaa agatcatccg atccaatcat ttcaaaaaat gcaaaactga tatcaggatc
    21301 atttagctca ggcttgttat cgtaaagcat ttgtaaaggt tgcatttagt taggagtaaa
    21361 cgcgaaacta tcatcattat ttgctgaaga agatgaagaa gctaattcag agtttgtttg
    21421 aaataaagga gacgtgctga cagtttcttt accctgtttt ttgcagaggt agaaactttt
    21481 atatcggaag tttctaccgg agaatttttc tcatttgtag aaattgttga atcgatacta
    21541 ttcgaaattt gcgtatctga aggtatgttc tttgatgcag aagtcccaca agcagtaagt
    21601 aacaataaat ttgataaact gataaacaga attcttttca ttatgtacac actccttata
    21661 gtatcgttta gcatacacct atgctcagta ttaggcaaaa tcaatgcaaa actatcaagt
    21721 cttttatagt atctattagt cttagtaata catatatgga gagggtgata tcccagttca
    21781 aaccagatac attggttacc atctgtatta acgattatgc ctctaaacgc tttttttatg
    21841 aaaaaattta taagtgaaaa cgtaggtatg atattatcta gaggtaattt ttttgggtat
    21901 ttaaatagtt gctaggagga tag
//
LOCUS       DANKBF010000049        21903 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000049 DANKBF010000000
VERSION     DANKBF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            133..1137
                     /locus_tag="PWY89_001932"
     CDS             133..1137
                     /locus_tag="PWY89_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066969.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1138..2082
                     /locus_tag="PWY89_001933"
     CDS             1138..2082
                     /locus_tag="PWY89_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2066970.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2082..3044
                     /locus_tag="PWY89_001934"
     CDS             2082..3044
                     /locus_tag="PWY89_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2066971.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3071..3985
                     /locus_tag="PWY89_001935"
     CDS             3071..3985
                     /locus_tag="PWY89_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2066972.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4011..5792
                     /locus_tag="PWY89_001936"
     CDS             4011..5792
                     /locus_tag="PWY89_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2066973.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6140..6826
                     /locus_tag="PWY89_001937"
     CDS             6140..6826
                     /locus_tag="PWY89_001937"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2066974.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6846..10427
                     /gene="smc"
                     /locus_tag="PWY89_001938"
     CDS             6846..10427
                     /gene="smc"
                     /locus_tag="PWY89_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL2066975.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10436..11245
                     /locus_tag="PWY89_001939"
     CDS             10436..11245
                     /locus_tag="PWY89_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2066976.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11256..12254
                     /gene="ftsY"
                     /locus_tag="PWY89_001940"
     CDS             11256..12254
                     /gene="ftsY"
                     /locus_tag="PWY89_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2066977.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12441..14708
                     /gene="gshAB"
                     /locus_tag="PWY89_001941"
     CDS             12441..14708
                     /gene="gshAB"
                     /locus_tag="PWY89_001941"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2066978.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14797..15249)
                     /locus_tag="PWY89_001942"
     CDS             complement(14797..15249)
                     /locus_tag="PWY89_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL2066979.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15399..16349
                     /locus_tag="PWY89_001943"
     CDS             15399..16349
                     /locus_tag="PWY89_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2066980.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16342..17301
                     /locus_tag="PWY89_001944"
     CDS             16342..17301
                     /locus_tag="PWY89_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2066981.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17298..18053
                     /locus_tag="PWY89_001945"
     CDS             17298..18053
                     /locus_tag="PWY89_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2066982.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18076..19029
                     /locus_tag="PWY89_001946"
     CDS             18076..19029
                     /locus_tag="PWY89_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2066983.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19673..19969)
                     /locus_tag="PWY89_001947"
     CDS             complement(19673..19969)
                     /locus_tag="PWY89_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL2066984.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20324..20680
                     /locus_tag="PWY89_001948"
     CDS             20324..20680
                     /locus_tag="PWY89_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2066985.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20690..21589
                     /locus_tag="PWY89_001949"
     CDS             20690..21589
                     /locus_tag="PWY89_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL2066986.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         7246 a         3862 c         4662 g         6133 t
ORIGIN      
        1 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
       61 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
      121 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
      181 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
      241 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
      301 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
      361 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
      421 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
      481 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
      541 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
      601 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
      661 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
      721 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
      781 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
      841 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
      901 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
      961 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
     1021 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
     1081 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
     1141 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
     1201 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
     1261 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
     1321 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
     1381 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
     1441 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
     1501 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
     1561 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
     1621 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
     1681 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
     1741 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
     1801 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
     1861 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
     1921 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
     1981 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
     2041 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
     2101 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
     2161 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
     2221 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
     2281 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
     2341 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
     2401 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
     2461 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
     2521 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
     2581 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
     2641 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
     2701 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
     2761 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
     2821 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
     2881 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
     2941 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
     3001 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
     3061 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
     3121 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
     3181 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
     3241 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
     3301 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
     3361 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
     3421 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
     3481 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
     3541 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
     3601 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
     3661 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
     3721 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
     3781 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
     3841 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
     3901 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
     3961 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
     4021 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
     4081 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
     4141 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
     4201 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
     4261 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
     4321 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
     4381 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
     4441 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
     4501 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
     4561 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
     4621 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
     4681 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
     4741 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
     4801 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
     4861 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
     4921 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
     4981 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
     5041 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
     5101 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
     5161 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
     5221 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
     5281 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
     5341 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
     5401 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
     5461 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
     5521 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
     5581 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
     5641 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
     5701 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
     5761 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
     5821 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
     5881 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
     5941 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
     6001 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
     6061 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
     6121 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
     6181 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
     6241 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
     6301 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
     6361 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
     6421 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
     6481 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
     6541 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
     6601 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
     6661 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
     6721 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
     6781 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
     6841 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
     6901 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
     6961 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
     7021 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
     7081 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
     7141 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
     7201 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
     7261 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
     7321 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
     7381 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
     7441 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
     7501 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
     7561 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
     7621 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
     7681 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
     7741 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
     7801 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
     7861 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
     7921 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
     7981 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
     8041 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
     8101 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
     8161 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
     8221 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
     8281 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
     8341 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
     8401 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
     8461 aagcagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
     8521 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
     8581 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
     8641 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
     8701 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
     8761 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
     8821 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
     8881 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
     8941 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
     9001 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
     9061 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
     9121 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
     9181 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
     9241 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
     9301 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
     9361 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
     9421 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
     9481 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
     9541 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
     9601 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
     9661 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
     9721 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
     9781 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
     9841 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
     9901 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
     9961 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    10021 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    10081 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    10141 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    10201 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    10261 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    10321 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    10381 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    10441 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    10501 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    10561 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    10621 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    10681 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    10741 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    10801 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    10861 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    10921 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    10981 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    11041 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    11101 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    11161 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    11221 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    11281 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    11341 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    11401 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    11461 gttttgaaac agcgatacga atcaccgaag cattacgtca agaagtgaaa ctcagaaatg
    11521 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    11581 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    11641 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    11701 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    11761 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    11821 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    11881 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    11941 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    12001 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    12061 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    12121 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    12181 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    12241 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    12301 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    12361 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    12421 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    12481 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    12541 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    12601 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    12661 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    12721 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    12781 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    12841 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    12901 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    12961 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    13021 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    13081 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    13141 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    13201 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    13261 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    13321 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    13381 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    13441 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    13501 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    13561 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    13621 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    13681 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    13741 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    13801 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    13861 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    13921 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    13981 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    14041 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    14101 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    14161 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    14221 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    14281 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    14341 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    14401 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    14461 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    14521 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    14581 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    14641 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    14701 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    14761 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    14821 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    14881 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    14941 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    15001 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    15061 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    15121 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    15181 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    15241 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    15301 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    15361 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    15421 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    15481 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    15541 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    15601 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    15661 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    15721 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    15781 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    15841 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    15901 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    15961 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    16021 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    16081 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    16141 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    16201 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    16261 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    16321 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    16381 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    16441 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    16501 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    16561 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    16621 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    16681 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    16741 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    16801 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    16861 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    16921 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    16981 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    17041 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    17101 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    17161 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    17221 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    17281 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    17341 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa gttgactgct tttatcggtc
    17401 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    17461 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    17521 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    17581 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    17641 tcgttcagga atcactggaa cacattgggt tagaagaaat agcggatcaa tcgattgaca
    17701 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    17761 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    17821 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    17881 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    17941 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    18001 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    18061 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    18121 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    18181 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    18241 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    18301 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    18361 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    18421 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    18481 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    18541 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    18601 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    18661 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    18721 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    18781 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    18841 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    18901 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    18961 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    19021 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    19081 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    19141 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    19201 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    19261 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    19321 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    19381 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    19441 ccagcgttct tattactcaa aacaaatcgc agttgtcaca atctcttcta aacgatgttc
    19501 catctgctgc ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg
    19561 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt
    19621 caatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    19681 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    19741 gcttactcca ccgttttagt aattgataga aataactctc ttctgctttg attttatact
    19801 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    19861 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    19921 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    19981 caaagaagtc ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac
    20041 aaaaagtaat tctttttcca atagttgtta aaggtttttt attttttata gtattttttg
    20101 ttatttaaga aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt
    20161 tcaagaaact gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat
    20221 aaacgaatga cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca
    20281 tctgatgaat aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac
    20341 aaaagaagca caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga
    20401 tatcgaaaac ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc
    20461 atttcgattg atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt
    20521 ggctgataca ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga
    20581 tccatccaca cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg
    20641 cttgagaaaa ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt
    20701 ggcgatcatc agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca
    20761 gctttggcaa gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc
    20821 taataaagta gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac
    20881 gacatttcat ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca
    20941 ttttccagac ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct
    21001 tttagggatg aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat
    21061 cgtacgaatg aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct
    21121 cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa
    21181 aaaacttgag gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca
    21241 atctgctcct tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg
    21301 agaattactt gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg
    21361 ctttgaagat aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta
    21421 tgaatggcag ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc
    21481 tatgaaatta cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca
    21541 acatttgcaa gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag
    21601 taatgtgtaa aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt
    21661 tatcgtggat atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat
    21721 gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac
    21781 aaaaaaaaat ataatataca ttttttattt aattataatt aatattgatt gaagtctagg
    21841 cagtcaagtc gagagtgtaa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    21901 gtc
//
LOCUS       DANKBF010000050        21500 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000050 DANKBF010000000
VERSION     DANKBF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            44..379
                     /locus_tag="PWY89_001950"
     CDS             44..379
                     /locus_tag="PWY89_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066987.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(636..2219)
                     /locus_tag="PWY89_001951"
     CDS             complement(636..2219)
                     /locus_tag="PWY89_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066988.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2341..2925
                     /locus_tag="PWY89_001952"
     CDS             2341..2925
                     /locus_tag="PWY89_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066989.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2936..3181
                     /locus_tag="PWY89_001953"
     CDS             2936..3181
                     /locus_tag="PWY89_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066990.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3187..3909
                     /locus_tag="PWY89_001954"
     CDS             3187..3909
                     /locus_tag="PWY89_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066991.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            4040..4783
                     /locus_tag="PWY89_001955"
     CDS             4040..4783
                     /locus_tag="PWY89_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066992.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4789..5154
                     /locus_tag="PWY89_001956"
     CDS             4789..5154
                     /locus_tag="PWY89_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066993.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            5170..5700
                     /locus_tag="PWY89_001957"
     CDS             5170..5700
                     /locus_tag="PWY89_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066994.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5862..6170
                     /locus_tag="PWY89_001958"
     CDS             5862..6170
                     /locus_tag="PWY89_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066995.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            6166..6585
                     /locus_tag="PWY89_001959"
                     /pseudo
     CDS             6166..6585
                     /locus_tag="PWY89_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6722..7522
                     /locus_tag="PWY89_001960"
     CDS             6722..7522
                     /locus_tag="PWY89_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066996.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            7559..7741
                     /locus_tag="PWY89_001961"
     CDS             7559..7741
                     /locus_tag="PWY89_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066997.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7852..8169
                     /locus_tag="PWY89_001962"
     CDS             7852..8169
                     /locus_tag="PWY89_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066998.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            8172..8404
                     /locus_tag="PWY89_001963"
                     /pseudo
     CDS             8172..8404
                     /locus_tag="PWY89_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8519..8998)
                     /locus_tag="PWY89_001964"
     CDS             complement(8519..8998)
                     /locus_tag="PWY89_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2066999.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            9118..10455
                     /locus_tag="PWY89_001965"
     CDS             9118..10455
                     /locus_tag="PWY89_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2067000.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            10478..11005
                     /locus_tag="PWY89_001966"
     CDS             10478..11005
                     /locus_tag="PWY89_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067001.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10992..11555
                     /locus_tag="PWY89_001967"
     CDS             10992..11555
                     /locus_tag="PWY89_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067002.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11977..12714
                     /locus_tag="PWY89_001968"
     CDS             11977..12714
                     /locus_tag="PWY89_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067003.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(12731..13147)
                     /locus_tag="PWY89_001969"
     CDS             complement(12731..13147)
                     /locus_tag="PWY89_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067004.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            13540..14787
                     /locus_tag="PWY89_001970"
     CDS             13540..14787
                     /locus_tag="PWY89_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2067005.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            15055..17445
                     /locus_tag="PWY89_001971"
     CDS             15055..17445
                     /locus_tag="PWY89_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDL2067006.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            17494..19329
                     /locus_tag="PWY89_001972"
     CDS             17494..19329
                     /locus_tag="PWY89_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2067007.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            19398..20261
                     /locus_tag="PWY89_001973"
     CDS             19398..20261
                     /locus_tag="PWY89_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2067008.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            20251..21360
                     /locus_tag="PWY89_001974"
     CDS             20251..21360
                     /locus_tag="PWY89_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2067009.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
BASE COUNT         7945 a         3004 c         4163 g         6388 t
ORIGIN      
        1 aatttcctag gaaaaatagt taacataaaa ggaatgaatt gttatgaaac aatatttaaa
       61 tgaatggaaa gcgatagagg ggtcattagt aacgcaaaga ataaaggaat tacctgattg
      121 cttagagaag gatcatttgt ttcaaatacg agaaatgttg aaaaaagaac agtttgatcc
      181 agaccaattt ttagtagtcg agtatccaac tattggagtg tactgttgta accatatcaa
      241 tgatggaaaa tattttatta ttcaggagta tggaggacag ttaactcctt tctatactac
      301 atgggaaatg agtgaagaag gaatcaataa ttttccatgt gaaagcatcg aagattcaat
      361 ttcacacacc gaatgttaat tgattataac tataaaaaag gattgaacat tatgttcaat
      421 ccttttttca atgaaatgtt ttccgccatt tcattgtttg gtgcgatttc tattttatta
      481 tatcaaaata aagagagatt ctaaagaaag aagagggaaa tatataaatc aagcgacagt
      541 atatttatta ttaaatgcta ttgtgagaat ataactaatt agtttaattt gaaagtaaat
      601 atacgctata aaagtgcatc ttgtgttata aaaaattatt ttattttgtg aacataattt
      661 aacaattctt tctcggaatt gatttctttt cttaaatagt cttcagactt attaataata
      721 ttatctatta tgtctagata catatcactt ttcgattcag ctaattcata cagttttttt
      781 aattctttat cctttaattg aaaaccctga tccatagtca ctgcatttgg atatagaagc
      841 ccactataat gtttacgtaa attcacttgg aatttctgat tataaaagtc taacagatta
      901 gaaataaaag gcggaaaatt actgtttttg taaaattgat tatttccatt taggttgtat
      961 agtaatttgt taaattgctc ctgatttaat ttattgtaaa tccatggtaa tacttccata
     1021 tccttatctg ctaacctgaa tgaactagca tcggtaagat gttcgatcat aaaatctatg
     1081 aacatctctt tttcaccaaa atataaatat tgattatata ttgtttctaa tgttgaagat
     1141 ggtagcgaag aaactctcat tttttgctgg agtataggat caactagact atttaagtga
     1201 ttttgtaatt cttcattgtt gtttcctaga actgcaaggg aacgtaaacg gtagtaatat
     1261 tctatttctg ttagtacttg ttgtttcggt ttaaaatcat ttattgatat ttgtaaattt
     1321 tgaattgtct cgtctgaaaa atcatgttgt aattctggat gggtagctat aaaagtaagt
     1381 aaaaagaacc cttttgtata cgtgatctgt tgattttcac caagtgattt attaatttcc
     1441 tctcttgatt cagctgtgaa cctatacttt gcaatttctt tttttagaat atttaaatag
     1501 aattctttat tatcaaaaat tagggcatta agtagtctga aagtactttc cctatctcta
     1561 tctgcttcct catttccata agataattga aaaacaaact taaaggctgt cttacatagg
     1621 ttttctttgg ctttttcgct tagtttattt agatagctat tttttagtgc agagtgaaat
     1681 tcttcaggtg taacagacat agcatgtatg cgacttgcat attgtataat ttcgatcgtt
     1741 actttttttc ctaatattgg attttttagg aatataattt cataggactt ccgtataaca
     1801 tcctgacaag tttcttttgt tatatctctt aattcccaag tattaccgtt gtaagtgact
     1861 acaggatgag ctgaataatt tctttgatcc ttaatatatt ttaaatcctc atattcatag
     1921 ctatcaatta gctcagttga actataagct ttttctagta gttccatttc ccattttgga
     1981 gagtttggat cactttttct tagtctagct atatcttcta aaattttctt agaagtatta
     2041 tcctcgtaaa tatttgctaa atcagataat ttatcaatta aatctaaaat aagcgcactg
     2101 taattgacag ctattgcaga acgataattt ccctgataaa aactacttct aacttcttct
     2161 aataatggaa taattctagg atttttaact ttttttattt catcatctaa tataaacata
     2221 taaatctcct ttttttattt gtatgacaag tttaccataa aaaaaaatta aaaagcgaga
     2281 catatttatg ttgttgtgtt atcataataa cataaatatg tagagtgaga tgatgaaaaa
     2341 atggatttga aacaatcgtt ttacatctgg ttatatcctg ataatcctat gtcaataatg
     2401 gctgatattt ttgtagttag catcttattg tatttatttt atctaatttt ctttcacaat
     2461 ttaatcgggt acatgattct tgattggaaa ggaatgttcg atatagaaag aaaaccattt
     2521 gctaacaatc aaacctatac cgtcttcgaa agtggagatt gggaagaaaa tgaagttgat
     2581 gtgatccatg aaattggatt agctttatca ggaatacaag atgctgatgt tttggtatca
     2641 atgacaacgg atactggata caacgcttat gaggtatttt atcttatgta ttttatatgg
     2701 cgtgatggtc aagaaattat caatggtcgt gctgaaaaaa caatcaattc taatactgaa
     2761 atttcttttt gtatgggtga aggggaaatg attgtatata aagatgggtc agcaaacatt
     2821 aaaacagcct ttggtgatat tgaaacaagt attaaggaac ttagggaata tgtgcagaac
     2881 ccatataatt ttcaatattt agcaggaaga ataaatttag tttaggagta aaaatatgaa
     2941 aaaaattata aatcaaccaa caaatcactt gataattgaa ggtgttattt tcgtaacaat
     3001 gtattttact gtaggaaatg gcatctcaaa tacaatcatt caaaaaaatc cacaatacct
     3061 attatttagt ctttatgctt ggattgtaca aatggtttgg gtttccatat tgatgctgtt
     3121 gttttattgg atagtacggg ttattctttt attgttaaac aaaagaaaaa aggataggtg
     3181 aataaaatga aatcaactaa attgaaggtt gtattgttat cgacagcagc attaataacc
     3241 cttacagcat gtggtacaac taatagtgct aataataaca aagacacaaa gtctagtagt
     3301 acgatggtta ctaaaaacag cactacatcg gcatcaacaa caatgtcttc aatgactgaa
     3361 aataatgctg aaaagtatca aaaatacgaa gacattaata aaggtcaaaa gccggatgaa
     3421 gtttataaca actttattaa tacagtaaaa aatgatgaaa attatgggaa tgatccattg
     3481 caaaataaaa atctgactat cgttctttac aaacctgatt gtccagattg tcaaaaagca
     3541 gaatcaaaaa tcatgcaaga aataaaagaa ggatttagtt atgctccaac attaatgcat
     3601 gatattacca taaataaacc agcttacgag tattacgcag ttgctgtaga tattactgat
     3661 ggtgtacctg agtgggtaca aaacattcct ggtctggctg atgagaacaa tcaatatcat
     3721 acgccaacag ttgcagtagt aacaccaaag aaagttaacg gcacactata ctgggatgta
     3781 atgggaatga caacaaatac aactgatgaa atgattgaaa gtacaactaa atattatatg
     3841 gatttgggag catataaatt atatcaacaa tcagagcctg ttatggacga tcaatccaaa
     3901 gttaaataga tctttagggc gtaagcccta tttttttaaa attaagtgat aacaaataaa
     3961 tgatttctga aacaaacgtt cttactcaac tactcgaaac acacaagcta attaactaga
     4021 ttataaagga gaatgcgtaa tgaaagaaat cagtgcaaat gaagctcata ttccgcaaaa
     4081 gaatcaggtt ggtatatata aggtgcttca tggtgaagga atttataaaa tacagttact
     4141 taccggttac aataatggtg caaatgggaa gtggttaaag gcaacaagta ttcgtgaact
     4201 tagaagtgaa gtagaaaaaa ttaggcttgg tctcacaagt gaagaaatag ctaaactgaa
     4261 gggaaataat tcttttgtaa ttgaaaaaga aaaggaaaaa catctacttt atcgtgagct
     4321 gaaaattgaa gaatactatg aaattgattt acctgaaaaa ttaagaatta ttgatcaacg
     4381 ttcagaagaa aaatggaaaa ctgaaatata tccaagctta aatgactaca agacatcagg
     4441 aaagcccatg tataactaca caggcttttt aacctacaca caatacaaat atgcctttcg
     4501 caaattaaaa ggcattaaac aagctgaaat tgccctgtat gagatgaaaa aagtggatag
     4561 attgaaattg attcaagatt tagtgcaaaa tacatggaat caagcgattg tcgagctaga
     4621 aaaagaaaat gggaaaattt taactaataa atatctgatc gtaaatgaaa ttgaagaatt
     4681 aattggttta ttttcatgtg aaaaaggtga agtcttcttg aagtcatcac caacctattc
     4741 aattttggac agtccgtatc agtttataca ggcaaggaga tgagttagat gtctagcttg
     4801 aaagatgact atatccaagc aaaacaagag tatgaaaaaa tacagatgtt gaaatctgag
     4861 aaagtcgaat tatttttaac acaaaaatta gcagaggatc atattgctaa cttagatgct
     4921 aaaactttag aggaatatga tcggcagttt aatcagatac atcacaatat cattttgctt
     4981 gttgagcagg caaagcgaag gtttgaaaaa gcggaatatg atctattaga tcaaggaatt
     5041 gtatgcttgg ataagggatt gcgaagatat tatggtgcag agttaaaaaa gcaaaatgta
     5101 gatagaaatg cactaaaaca tgaaatctta gaattgataa tgcaacgaat ttgaagggat
     5161 agaaagcaaa tgtcacaaaa cattagtgct gtaaaacaat taatagaaga agatttagag
     5221 accacagttc agagattagc ggaacgaatc cattttgcaa attatcaaac catctctaac
     5281 tggtcaagca ggaacagaaa tccggaagaa attccaatga aattttttta tcgaatcgga
     5341 aaagaatatg gtgttgatcc gataaaaatt ttcgagaaga ccatgattta ttactatcat
     5401 ggaacaccta acgaagtgtt agaacatctt ttggaagagg caaaagaaaa atcatcaata
     5461 ttagagctat cgcaaaatga caaagttatt caagtgaaat gcgttgatcc aacaggaaag
     5521 attattgtcg gttttgatat tgaaagaaaa tccaaaatag attttattac acaattaaaa
     5581 aagataaata atttagatat tctaacgctt agaccagatc atgaacagtt gtttatgagt
     5641 tttaaaaaac aattgaaaga aatacaaaaa agatataaca aggtgtttcc taatgcgtga
     5701 aaagctcaaa gaaatcagag ttttttggat aattaacaaa gaatgtatca tcttatatac
     5761 tcttggattt cttagcatga tcttgttttg gtggttatat ttaaaacagt aattggagag
     5821 ggtcaagagg atttttatca agataaggat gtggaaactg catgtggaat aatagattaa
     5881 aaacaggatt gttattaata gttatttcct gcgctatgat gattggtatg aggatacaaa
     5941 gggaacaatc ctattttgaa gtttctgcta acaacgtaat agaaaaatgc tattacggtc
     6001 aacattattg gagtgaggaa gtcagagaaa atatagatcg tgaatatgtt caaagaattg
     6061 tttgggatgc atattctatc aaagattatc ctaaaagttt aacgagtaga ttattttaca
     6121 gtgaaaaaga taatcaaaaa ctctcagatt tgatgatgaa aaaagtatga ctattattac
     6181 gaaagagaaa cagagtgcta tattcgaggg gaaaaagcct acaacaaaag tgactattgg
     6241 ggtgacaagg cgaagcgatt aactgcaaat tttagagtgt atgaatcaga gtttataaac
     6301 ttgacctttc ttaatagtgt ttttattgat tatgtaatta ctacgaaacg cattggaagt
     6361 tattcaagta cctacgcatc cttagttcca atgttaaact cgatgagccg tttcttaaaa
     6421 gaaagagaaa agaaagagct acagtatatc aaagaaatca tgccggattt tgaaatgcac
     6481 gaatggcaaa ttgctttatc agattggaaa ttggcgaata aggttcgtag aataacacct
     6541 tttcaagcca agcgcttttt gagagctatg aaatttgtgc aataaaaaaa gccaccagac
     6601 aagttgatgg agactaacac aattaccaac agttgtaatt gtgttagctt acaaattaat
     6661 tatggtgttt agtcaatccc aaattgtgaa atggcaccaa taattggaaa ggaaaaatga
     6721 aatgtctgaa gatttacgag agctgaagtt acgaaagaag cagtatgatc agtatttttc
     6781 cggtttgttg aataaacata ttgaagaagg acttgatcat gattttggaa aagatgaata
     6841 ttgtttatat tgcgaacgtt acaatgaaaa aatgtctgaa tatctacagc tgaaaaaaga
     6901 gatacagaaa ttagagaaaa aagtacatgc ttggaagtat aaagattcca tgtctggtta
     6961 tgttgaatct caaaataagc caagaaaaaa gaagcaagcg tatgttctaa agatggtaaa
     7021 tggggaagag ttaattgtat tagcgtacag tgaagaagag gttcgagaac tcgttgaaac
     7081 acctgtcagg aaaaaggttg aaacaataaa tgagatagac gacagccaat acaatagcat
     7141 ctatattgaa acaagtcatg gtggatataa gaaattagca actttcctta gaagttctaa
     7201 agttgggata atttctaaaa aagacataag tgctgttacc gatagttttt gtactttatc
     7261 tgttcctaaa gcttatttcc aatacgatag taaaagattt gaacgcttag agaaattctg
     7321 tagaccgtat actgtattga aaactaaaaa atttaataaa actaaaagag tttctataca
     7381 gttaccgact ggtcaaatct cattaggtga aaaagactat ctgataaaag taggaaaaga
     7441 gttaattata aaagtaccgg aacacttatt taaagaaaat tttgagatac gtgaaacctt
     7501 ggtatggaaa gaagaaaaat agtttggaag acatagaatg gtataagggg aaattgaaat
     7561 ggcagtaaat aaaatattgc aggtactaat catagacgaa tttggcggag gagaatggtt
     7621 tgatgtgcag tccatagact ttgaagaaga agtcgtagtt atagaaaata tttacggcga
     7681 agtatattca tattcgcttg aaggtgagaa tattgaggat ttacggttga ttggtgagta
     7741 gaaaaaaaca gaaatatcaa aatggagata aaaattaata gcatgtaaag atagtcatgc
     7801 ttgggttaaa aatgttaaaa ttcaaaaaaa tgagtcagaa gggataaaag tatgaaaaga
     7861 gaggaataca aacaaagatt aaacgaactg cttgaagagg acgaaacatt aactcatggt
     7921 tcgcctgatg aaattctata catgattgat aatatggtga tctttggtgg atatgaactt
     7981 ggtaataggt cagttgatca taatatttta gaatttgatg atgtatcatg ggaagaaatt
     8041 cttgattggg gaattttggc ggtacctgaa actaaaacct atattagtga cactatggtt
     8101 cctttttttg aggaattgga ttataaaaga cttcccaaaa atgaaaatca tatactggga
     8161 ggaaactaac aatgttaagt gacttattta ttttactgat ctatctaggt gtaagtgaaa
     8221 ttattgcgta ttttttgttt gtcattgcta attatggtat ttccagtgca atcgttcaaa
     8281 tgtttagatt accagtaagt gaactaaaaa aatgatgaga ttatcagcat atcttttaat
     8341 gtttttgaat gtggtcacgt taatctttgt agtaatcggt gtgactttcg atttaatgga
     8401 atagtgggaa atctataatg gaaaaaagag acacgaatga acatattttt tgaatcaata
     8461 ttgttgatgg tttttgcgat cagcgcttac cagaaatcta aaaaagagta atgattgttt
     8521 aatcatcact ctttttttgt agggaaattg aaatatgtgg agcttgtatt ggtttagtta
     8581 ggattgcttg gctataagga gctagttctt tatcaaaacc atcaaagtag gttttcagta
     8641 gcgatttaac tgatcgctca tcttgtttga tttggataat ttcagcatcc aaatcggttg
     8701 tgagatcagg agcttttgat gcgattacct gatttttttt atcttccaac tctcttaggc
     8761 tgtttaacaa gttgtaaaaa gaatcatttt gattgttcat tccaacgtga gttgcaattt
     8821 taacagaagt ttcgtccgta ttgtcatcaa aagaactcat tggaatatca agtagtaacg
     8881 aggatgtttc ttttactgac tttttcttta cagcattttt tgtttttcca tcttttttgc
     8941 tctttactcg agatgttttt ggagctagtt taggtaaatc acttgtttta tcagtcattt
     9001 attagctttt cctttctatt aatttatgtt attatattat cataataaca taaaaaagta
     9061 cactttttgg gggcgtagtt attgaaaaaa tctaagagaa agattgccac attgcttatg
     9121 ttaatggtgc aatttacagg gattgcaatg agtgtaatca caataaatcc gcaagtgata
     9181 catgctgatg ggatagctac ttatgatgca caatttaact gtactactga aggtagtggg
     9241 tcaggaacag atagcgatgg aaacgatact ggaagtggcg ctgtaacagg tgatgtagcc
     9301 aaaaatattg aagatattta caaatacatg aatggagaat atggcttatc aggtgctagt
     9361 attgcaggta ttttaggtaa ctggaaacaa gaatcaggta ttgatcctcg agcagtagaa
     9421 gggtattacg gagatggaca gagagaagtt gacttcatca aacaatttac tgatggtctg
     9481 atgggtattg gctatggtca atggacagct gggcgacata cagcattagt tgattggtct
     9541 aagaaaaata atggcggttc ttggtggact acagaagccc aattggactt tatgttcaaa
     9601 ggtgatgggg gattcgtagg aatattaaaa gattatgctt taaatgcttc tgatgatgtc
     9661 gtagaaaatg cagtaacttt tcataaaaac tgggaaattt cagctgattc cgaatcagct
     9721 gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga agtacatgaa aagcaagggt
     9781 atggacggtg caaaagatga aagtaagatc aataaaatat ccggaggttc aagtaccgga
     9841 gatggatcga ataattcatc aagttcagca tcaggaacag tagaagatgc ctgtgcaact
     9901 acggatgacg ctaattctgg ttctggatca attgggaaag aaggaaaaat taacggtaaa
     9961 aaaggtcaag taatagcgaa tatgacttat gaagaagctt tgaaaaaata tgggaaagcg
    10021 atcacgctac caaaattcaa agaaccagat tggtcaactt caccttttaa cggaatatat
    10081 caaggtcagt gtacagagtt gacgtgggct tatatgagcc aattatacga tgggaatcaa
    10141 ccgactactg gaaatggtgg ttatgtatgg gaaagctaca aatcggcagg agcaaaaata
    10201 acgaacaaac caatcgtagg ttatgggttt agtgcttcgg atggatggtt atgggctgtt
    10261 ggagatgcag ggcatactgg tgtagttata gcggtgtttg aagatggatc atttctatgt
    10321 gctaacttta atgttccacc aacccaagct ccaacaaggg gagtttcggt aacgttgatt
    10381 gatggagtgg acggatcaga taatgtgcat ttctttagcg gtgtaggaaa agctaaattt
    10441 gaaatcgaaa aataaattgg aatgaaggac gatatagatg aatagtaaag tgaaagatac
    10501 aattatcatt tctttgcttt ctggtatgtt attagcactg cttgttggaa tcctattctt
    10561 caataagcag aatcaggaca tagaagctga aaaacacagt gtgcatattg aagataagct
    10621 gacattcttt aaagaggtta aaccaaatga ggtggatgga ttacggaaag atttagaaaa
    10681 tgatattcaa gaaatgttga atggatcagt tcaaaataag tttgcagatc aatcaggtga
    10741 agaatttttg agaaaactat ttattgtaac aggtaaacca ccaattactg ataagagtag
    10801 tgagaaagaa aaaatcgagt actataagcc ttttactttt aagctaacaa actttggaat
    10861 taagacatct agtagtgggt tgtatgaagc attgaccgat tttgaagttc aatacaatgg
    10921 aaaaaaagtg tatgatacca cattaaccat cacactaaat aatgataaga aagtgactgg
    10981 gggtgctttt tatgcaaaat catgaaatca ctactgctaa aaaggcgatc ggaatacttt
    11041 ccataattgt gatcctttgt tgtgtggtac tatttcaaaa gaaaaatgct tatgataata
    11101 atgtcaaaga attggaagac cttaaaagta aacaaatcga agtagtcaag aataaccaga
    11161 aagtgaaagc tatgcaggaa gctcaataca aagaattggg aaatgctgaa attaaggaac
    11221 aaagtaggaa attctttaat ttattttaca actggaattc atgggaaaaa tatacgaaga
    11281 atatgaagga aatacaaaaa gaatttcctc agatagtaaa gtctagtcct gttgatattt
    11341 ctggcacgat ggtaggcact ggtaatagtc caatatctag ctatactgct gattactata
    11401 caacaaatca aaaaggtaaa atggttgaat tgattacaca gacaagaaat actccaacaa
    11461 cagcaacaac aacaatttgg cgtggagtaa caagcatagg agaaaatcaa ttgttttatt
    11521 tggaggattt agatagctat gtaagagcag agtaaaataa aaaaagagct aacggtacca
    11581 acgttagctc tttgtgctga cgatgataac aaaacctacc gaaaaaggat tgtttcatca
    11641 caaacacaaa cagaaaagaa ttgataaaaa acccgaacct cagaattttt attaatcaat
    11701 tcatttagat tataataaat tgagctgaac attacaatac gtatatgttg taaaacgtaa
    11761 aaaaagtcaa aagtaaatac tttcttaaaa agtcaaataa aactattaaa gacaatatat
    11821 atggtttatt tgacataaga aaatggtcta gatgataatt gtaatataga attgtagact
    11881 taaataaagc ataaaatacc ttttatgata aggtatagct cttaatttta agcagtttta
    11941 gatataagat tgaaagtaaa gtgggggaga ataataatgg cagatcataa attagaaaag
    12001 ttattagaag aaacgtggaa tccaaaagaa tttagtgaat tttttatgga gaattttgaa
    12061 actgacttag cagtgattgt taaagatgct cttagagagc aagggtatcc tgaaactgca
    12121 aactatataa acattaattt tactttgtat actgaaaata aaggaacttg ggatttttgg
    12181 gctactttag caaataagga gttaagtgat aaatcagaca caggaattcg taactttttt
    12241 gaatcaaaca gagatgatta tatgtatgct aacaatcaaa ataaattgaa ctttagagta
    12301 gagtttgatg aaacacctga agaatttata gaaagacaac cgcctaaaga aaatgtagca
    12361 aaggtattgg aagatcgttg gaattcagat gaaatagttt caacaattag cgaactaggc
    12421 ggacaatatg aaccgctagt agaagcggtt agagaagagc taagactgaa taagtttcct
    12481 gatgttcaaa atattgatgt ttctcaaatt gaaataaacg tcaagatcac gaataaactt
    12541 gattatggta gttgggcaga tatagcatta gaaaaataca tctactcaac gttaaaagag
    12601 tttatcgaaa atagaatgga tatcatgtat ctacagcatc cacagtattt aaactttggt
    12661 gttgaaatag ctacacctct tgaagaatgg aaaatggagc aagggcttga ctagcctttg
    12721 ctccattttt ttattttaca aatgaataca gttcgtcgtt tttaccaaga atgattgttt
    12781 gcccaacttt gagattgtca ggtgatcctt cagcggtttg taaggagaac gttccattga
    12841 acttatcgga taccgttata ggtagcttta attctgatcc ttcctcatct ttgagaattg
    12901 ctgtttttcg ttcatcaaaa taaacagtct ttattaaata atttttcgta gtgatctcag
    12961 cagatttgtc tttttttgta gtagtaccag tttctttaga attagaggta ttttctacga
    13021 ctattccatc tttggcattt gagcttgtgt ttatttgatt gaatattttc agcgacaaga
    13081 taacgctaag aatagtcaat atagaaacaa gtgtaattaa tactgataat ttcctttttt
    13141 tgttcatttt ttcttaacat ccttttttgt tttgattagt tagatattcc ttatctaaca
    13201 agccttttta ggctattaaa tgaagataaa ccccaaaaaa atattgcaat tatgacagtg
    13261 caatggtact attattatac aataaatttt gcgaaagggg taggtctaaa tcgcaaacaa
    13321 aaagaaattg aacctagaca aatacaactt gtataccgca agggaagcaa gtttgatttt
    13381 gggtaaaggt agaaattatg ttacaaacat aatcaactat tatccaaatc gtttgcctaa
    13441 aggttcgttc aagaaagtgg gtgacaaaac ctatattata acggaagaag gaatagaagc
    13501 attgcgatca caaacagaaa ataaaaacaa aaagaaggaa tgaatatgaa gagaaattcg
    13561 cttggtatgg ttttattggc atcagctgta cttgcggggt tatcagtacc agtaacctca
    13621 catgctgatg aatggactgc aaacacaccg gatcaaatcc aaattacaac aggacaatca
    13681 agttatgcgt tcaaaaaagg agatactatt tggtcaactg tagagaagct taaaagctta
    13741 ggttacaaga ctactgttgc tgagtttgcc gaattgaaca atgttgattt ggcaaaagga
    13801 gaacaacgga ctatcccgat tggacgaact ttcaacatca gtaaagatac attaacagtt
    13861 aaggaagcag atggcgaagt agttagtcaa actgttcaaa aagataaacc tgatcctatt
    13921 gaaaatacgg agcaagagaa caaaaaatcc gagactacaa aagaatcgga accaacagca
    13981 aacacttctg ataagacaga aacaagtgct tcaagtgttg atggagcaag ttcatctaat
    14041 acaaataatt ctacaagtga gaaagaagca tcacagggaa caagtgaaag tagcactacg
    14101 gaagctgata ctggaacatc aacaaataac tcacaatcaa catcaaatga tgcaaatgaa
    14161 tcaaccagta catcttctag tgatcaatca caaacaggtt cgagtgatga aactgacagt
    14221 tctacttcac caagtaaacc cgaacaacca actaatgttg tgctaggcgt tccatttatt
    14281 agtcaaggta acacaatgct ttgcgaagga acatctttgc tagaagcgtt acattataaa
    14341 ggtgttgcaa caaatcaaga tttaatgact tttgttaaat caatgccact atctcctgac
    14401 aataatccaa ataatggttt ttctggtgaa tggagacaca atgtggatgg aacttatcaa
    14461 ggtatgaatc cggctcctgt agtttcatgg ggtgttgcta atggcggaaa tgttgtagat
    14521 atttctggtt catcggtaca aggattaaaa gatgagattg taaatggaaa tcctgtagta
    14581 gcgtgggtaa cttatgcctt tgaacctgct caacataaac caatgttttg gggcgatgct
    14641 atttggaatc gacatgtagt agccgttgat ggatttaaag acggttttta ccatgttgtc
    14701 gatcctgtgt ttggggcaag ttggattaat gcaggatcat ttgaaacagc atataacgta
    14761 tctcaattag cggtttcggt acgttaataa aagatatcgt ttatatacta agtagttata
    14821 tagtctttat ttgtccttgc ttaactactt agaaaacttg ttgctcatgg tgggtagaac
    14881 taaaaggggt gatgctatga atactagagc gttgaaacag cttgctttca acgaaaaaaa
    14941 tgattaatag gtgtggtgat tttaacttag gaatgggcgc gaaggtcatt accacaccga
    15001 catatcgtgg ctcattttct tcgcctaact ggtataaaga aggagtaacg agagatgaag
    15061 catgaaaaaa cgataatttt aaaatcaaaa ataatttatt gttttgcgac tttagtaatg
    15121 atgttatcta cgcttataat gcctttttca gcaataagag ctgaagcaga tgtcacaaaa
    15181 ccgacaagtg caaaatttat agaaactggc gtaaaaacag acggttatat tagggttaat
    15241 atgccgaatc atccaaatga atggatgatt tcaagtcaat ttaaagattc tcatggaaat
    15301 attggatatt gtatggattc agaattgcca tccccaacag gaagtggagc cggaagcttg
    15361 aagtacaagg gagctggcag tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca
    15421 aaaacagcaa aggaactggg tgcaggaaat gatacagaag catggtatgc aacacaatta
    15481 gtttcatggg ttttagccgg aaactttaaa gtttcacaga ttgtatggtc acatccaaac
    15541 cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct
    15601 aagaatggaa aagatacgcc caacactgag ttttctatca cagcttctaa gacagctgac
    15661 gaaggaaaat atcacacttt cacatataaa acagcatcaa ataagactgg taatgcgaag
    15721 ctcactttta catcagcaaa accagctggt atgaagattt atgatgcaga tggtaaagaa
    15781 attactaaca acactgtgaa attaaattca agttttacta ttaaagtacc tgtgactaca
    15841 ccaagtggta ctttgtcatt taaaggtact gctaatgtta gcacaaccaa tccttttact
    15901 tttgatggac gaggtgtgta tcaagatgcc gtagttatga tcactacttc agaaacaaag
    15961 gactctaaat ctttgagtgc taaatggaca agagctgttg gtcaggtaac agttaagaaa
    16021 gttgatcaag atggaaatgc tttaaaagga gcggagttta ctctaactga caataacaat
    16081 gctaaatctg ttaagacaac cggagatgat gggttagcta cgtttgattt gcaacaaggc
    16141 ttaacataca gcttagaaga aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag
    16201 gatggtatta ctgttaatac cgatggacaa gaatttagtt acactgctac gaacacaatt
    16261 aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg gaaaaccatt agctaatgct
    16321 gaatttacct tgacggatga ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga
    16381 cttgcaagct tcgatattgt agcaaacaag acctacagcc ttgaagagac cggagtacca
    16441 actggttata caggcgtatt caagcaagaa ggaattaccg ttaaagatga tggacaaact
    16501 tttgaatata cagctacaaa cactttgaaa aaaggtttag taaaagttca taaagtcgat
    16561 caagatggaa aacctttggc aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca
    16621 caagtagaaa aaacagatga aaatggactt gcaagcttca atattgtagc aaacaagacg
    16681 tatagcctag aagaaaccgg agtaccagta ggttacacag gtgcatttaa gcaagagggt
    16741 atcaccgtta aagacgatgg gcaaactttt gaatatacag ctacaaataa tttgaaaaaa
    16801 ggtgtagtaa aagttcataa agtcgatcaa gatggaaaac ctttagcaga tgcagaattc
    16861 accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct
    16921 acgttcaaca ttattgcgaa caaaacttac aacttagaag aaacaaaagt accaacaggt
    16981 tacacaggcg cattcaaaca agaaggaatt accgtcaaag atgatggaca agtctttgaa
    17041 tatacagcta aaaacacttt gaaaaaaggt gtgatcaaag ttcataaagt cgatgatcaa
    17101 gggaaagcat tagcaaacgc agaattcact ttgacggacg acaaaggtgt aaaagttgta
    17161 caaaaaacgg acagaaatgg cttggcaaca tttaatattg ttgccaacaa aacgtacaaa
    17221 atcgaagaaa cacaaaaccc taatggttat acagggaaat ggtctaaaga aggcatcacc
    17281 gttgctgacg atggacaaac ctttgaatat acagtaatta atcaccttgt acctgaaaca
    17341 gtaagaacag gcggaatcgg tacatcagcc ttccttcttg tatttctaat tgcaggattg
    17401 agtatggtgc tactgtatta tcgagaacag cacaaagcaa aataaaaaag tgttaatgga
    17461 aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat caaaaaaaat tttttcaaat
    17521 ttaatgctat ttggtacttt gctaggagtg ggattgggcg caagctcaat ctcttctagc
    17581 cttggactag gggggcaagt agcatatgca caacaaaact ataattatca aggaactgga
    17641 acgattcata tccataaagt aacaaatact aatggggatg ggaaccctgt ctcagatatt
    17701 gataacaaag ggaaagaaat agctttacca aatggtgtaa aacctcttga aggcgttgag
    17761 ttctcttatg tgaaacttac taaagatgaa gctaaatcct tgggatggac ggatagtgat
    17821 actacaaaag gtcaacatat tgatgatgct aaagcacgat tattactaaa caaaaaatac
    17881 agtgaagtaa gtagaatgaa gactgattca caagggaata tagacatacc tatacaagta
    17941 aatacagacg atttaacaga taacttgtat ttgattgttg aaacaggaac agtcaaaggt
    18001 gtagttgaag aagcgtatcc tatgattgtt aacgtacctg aactaaacgg atcagatgat
    18061 caatacttta attatgatgt acatgtctat cctaaaaact atttccaatt aaactcagca
    18121 gagcttgata aaaaagacga agatggaaag gctcttgccg gaactgtttt tgaattgcga
    18181 aaagatgatg gatctgatca ggttgttgga acttataaaa cagacagcac aggtaaatta
    18241 actgtggatg gtttggaatt aggtgactat tatttcctag aaaccgattt gggaacagat
    18301 gctaatagaa caacctattt actagatggt aaaaaattca aatttagcgt taaacgtggg
    18361 gagactgcta aagtagttct tgatgttacc aactacaaac gacctgtaat tactaaggta
    18421 attgataaag aaaccgttga tactggcgaa gatgttcaat atacattgaa accatcaatt
    18481 cctggtaata ttggtaatta cgaaaaatat gttgtcaaag atgttctagg tgcagagtta
    18541 acttatgatt catcagaagt gatcgaagga atgactttag atactgatta cagtatcgaa
    18601 cgtgacggac aaacgacaaa ctatgtcttt actgaatcag gtatggataa gcttcagaaa
    18661 caatttgatg caggggatcg cttctttaca attttgatga acgctcatac taatagtaaa
    18721 gctaaaatgg atacaccact taagaatgat gtttcaacga tctacaacaa tggatatgaa
    18781 cccgaagacg aaacaccagt tgatcacgct tatacaatca ttggtgatca ccaattcatt
    18841 aaaacagacg gagatacaag caaagcattg agtggagcaa aatttgtagt gaagaacaaa
    18901 attgatccga ataaagttac ttacctaaaa caagatccaa caacgaaagc tgttacttgg
    18961 attgcagata aatctaaagc tactgaattc gtttccggat cagacggtaa gttccaaatt
    19021 gaaggtttgc aatataccta tgatgtagat tttgataaac aaggtaatcc aacaatcaca
    19081 gataaaaaaa tcaatgatta tgctcttgaa gaagttgttg ctccttcagg atatacacca
    19141 actagaaacg atgttccatt tgtggtatcg aatgatgatg cttctagcga agtaacagac
    19201 atcaagaacc ttaaaacacc tgaaacagtt cgtacaggtg gaattggaac agtagtattt
    19261 ctagtggttg caggtgtggc aggaacaggg ctatatgttg tctacaaacg agaacaacac
    19321 aaagcttaat gaaataaaat tccctagagt gtttttggct ctagggtttc tttttaaaaa
    19381 gtgtgtgaaa agtaggtatg agcatgaaaa agaagaatat caagttgatc atcttaaaaa
    19441 ttgggatgtt gctatgttta ggagtaggtc tatatcctgt aattactcag gcttatacct
    19501 actaccaaac tcaacaaaca ctaaactcct ataaggaaaa atatgatgaa gcttctgaaa
    19561 aggagaatga agaagtcttc aagagatatt cagaagttaa taggaaacga aaagacacag
    19621 aacaaacctc agtcacgatt cctgaagata ataacaatat tccgtcaaat ggaaatcttc
    19681 agaaggataa attaggtgaa ttagtgggtg aaattcttat accaaagatt aatgtggatt
    19741 tgccaattta tgatggtgta aacgatttcc aactatcagc aggagcagga atattaaccg
    19801 gaactgatta tccaactggt gggattggaa accattctgt tatatcagct cattcaggca
    19861 ttcctaataa aaaatatttt acagatttag agttgctcaa acttggtgat aaatttttta
    19921 ttgatatttc agggaaaaaa cttgcttatc aagtagatcg gataaaaaca attctaccaa
    19981 ccgaatttga ggatttgctt cctgtggcag gaaaagatta tgtaacactt atgacatgta
    20041 cacctgttgg agataattct cataggttac tcgttagagg ggttagcata ccttaccaaa
    20101 aaagtattgt tgagaaagaa aagaagaaag aagatttaaa ggtaaaggcg cagatgaact
    20161 ggtatcctat cgtagttgta ggggttttat taataatagc tgtgatagtc attattaaaa
    20221 aaatagtagc aaagagacga ggaatgaaaa atgaagaata aaatactact ttttttgact
    20281 cttatttgca ctttggtttt aacaatttat cctgcaaatc agctctatca tcaattcaaa
    20341 acaaaagaga atgtggcgga aatccaagag aaaattgaga aaaaacaaac tccttcaaaa
    20401 gaggaagtgg caaaaaaagt cgaacaaata aaatcgaaca gcggtaacca aggattagac
    20461 aatactcaga aaacagataa aggagtagac aatactcagg aaacagacgg tggatcattt
    20521 gatattcgtg aagctgaagg aaaggtcaat ccatacacag gagatgtttt ttcaaaaaat
    20581 caaacgaaag attttgaacc atctacctat tcaggagcgg ttggttatgt ctatattcca
    20641 aaaataaatg tgctagagct tttgtttcta ggcgcttcac aggagcattt atatagaggt
    20701 gtggcacaaa tgagtgctac tagtctacct ttatcaggta aaggtcatca aacagtaata
    20761 gcaggtcatc gtggcggtta tacgtcacca caatttcttt atgttgatca attagtagcc
    20821 ggagaccaca tttatataga ttatcttggt gaaaaagcag tttatacggt tttccgaaca
    20881 gacgttatta gaaattacgg tcagaattca acactgactg atattcctga tttagatatg
    20941 ttaacacttt atacttgtca tccatatcct cagaactacg atcgtttatt agttagaagt
    21001 ctaagagatg aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa taccgcatac
    21061 gctgatgcca ttgatagcga gtccgtttca gataagacag aaactgttac atcttctaat
    21121 gatttatttg aggatgttga tcgtgccaac aaggaattag atggaaataa tgatcttaat
    21181 attccggaac aaggtagcgt agagatgcaa gacagaacgt ttgctgaaaa ggcaagttca
    21241 atatttacag gaattaccaa taaacatcag aaaatgcttt tagtcatttg gactgtaata
    21301 ggagcattag caagtctaaa ggttcttatt cttttaatca aagagtttcg aaaagcataa
    21361 tgaagattat aaaaattgat agagtacagt aaaaagggat tgatactatt attgatctct
    21421 tttttatttt gaatagagct agattcactt ttaaataaaa tcaaaactga tcttaattct
    21481 aaagaaaaag tcaagaaaaa
//
LOCUS       DANKBF010000051        21346 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000051 DANKBF010000000
VERSION     DANKBF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            474..725
                     /locus_tag="PWY89_001975"
     CDS             474..725
                     /locus_tag="PWY89_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067010.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="PWY89_001976"
     CDS             759..1037
                     /locus_tag="PWY89_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067011.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="PWY89_001977"
     CDS             1634..1891
                     /locus_tag="PWY89_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067012.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="PWY89_001978"
     CDS             2004..2615
                     /locus_tag="PWY89_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067013.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="PWY89_001979"
     CDS             2794..3351
                     /locus_tag="PWY89_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067014.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="PWY89_001980"
     CDS             complement(4050..4571)
                     /locus_tag="PWY89_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2067015.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="PWY89_001981"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="PWY89_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2067016.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="PWY89_001982"
     CDS             8651..9538
                     /locus_tag="PWY89_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL2067017.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="PWY89_001983"
     CDS             9535..10704
                     /locus_tag="PWY89_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067018.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="PWY89_001984"
     CDS             10728..12083
                     /locus_tag="PWY89_001984"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2067019.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="PWY89_001985"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="PWY89_001985"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2067020.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14369..14659
                     /locus_tag="PWY89_001986"
     CDS             14369..14659
                     /locus_tag="PWY89_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067021.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14672..15697
                     /gene="sppA"
                     /locus_tag="PWY89_001987"
     CDS             14672..15697
                     /gene="sppA"
                     /locus_tag="PWY89_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2067022.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15700..16359
                     /locus_tag="PWY89_001988"
     CDS             15700..16359
                     /locus_tag="PWY89_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2067023.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16516..17448
                     /locus_tag="PWY89_001989"
     CDS             16516..17448
                     /locus_tag="PWY89_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067024.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17602..18891
                     /locus_tag="PWY89_001990"
     CDS             17602..18891
                     /locus_tag="PWY89_001990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2067025.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19138..20388
                     /gene="clpX"
                     /locus_tag="PWY89_001991"
     CDS             19138..20388
                     /gene="clpX"
                     /locus_tag="PWY89_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL2067026.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20471..21058
                     /locus_tag="PWY89_001992"
     CDS             20471..21058
                     /locus_tag="PWY89_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2067027.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(21132..21287)
                     /locus_tag="PWY89_001993"
     CDS             complement(21132..21287)
                     /locus_tag="PWY89_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067028.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         7076 a         3645 c         4601 g         6024 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgattaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccga
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc accacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagcactt
    12901 ggcggctgtc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacagga atctgtgtca
    13021 atttttccaa agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagcatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccgttgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccatcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 cattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctccttttt gttttttatc tggaaacatt taaaatcata accttttgcg aatgttcttg
    14221 tcaacaaaaa aagaataaaa aataaattgg tatatatcaa attctttgtc aaatgtcctt
    14281 tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc ggtacaatag
    14341 aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca tgacactggg
    14401 aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag atgatacgaa
    14461 aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg ccttggaagt
    14521 tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt tttccgggta
    14581 tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa ctgtgatcgt
    14641 cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg cagcagttct
    14701 gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta agcctcagga
    14761 aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac tagctccaaa
    14821 tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat
    14881 tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata aaaattttat
    14941 ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa
    15001 ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg atacgattcg
    15061 taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta gcggtggcta
    15121 ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga ccggctcaat
    15181 tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg gtgtggaaga
    15241 tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac cagagacaaa
    15301 agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt ttgtagcaat
    15361 cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg atggaaggat
    15421 ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat ttcctgaaga
    15481 tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag tagaatattc
    15541 ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag aattccaggg
    15601 attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag ggacagcgga
    15661 atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct ttcaaatgaa
    15721 aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga ctccaacttt
    15781 tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag
    15841 aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa agcaaatgat
    15901 tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt ggtcgatctt
    15961 ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct aggttggctt
    16021 aatttttcag attcttatct gagtctctac ggattcacag ccttacttat ttatctagcg
    16081 tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat gatctttggt
    16141 attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt agttagagaa
    16201 ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct tccagcagtc
    16261 tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt tgttacgatc
    16321 aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa aagcagtaac
    16381 agttgaaagc accttctaaa agtcagatct aaaatctagc ttttagaggg tgcttcagtt
    16441 atttttaatt gtcatgcttt ttttgttttg ctactattga acatgagctg aatatgagca
    16501 agaggaggac actgcgtgaa gccgattgat tttccagaaa aatatgagaa tttaatgcga
    16561 gtggcacaac aagcactagc aaaccagcaa tatcagcagg caaaagaatt atttcaacga
    16621 gcttacgagt tgaaagaaag ctttgaggct aatagcctgc ttgttttttg tctttacgag
    16681 ttggacgaga aaaaagaagc attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa
    16741 aatgaggagt ttgctgaatt ttattttgac ttgttaatca gtacacaaga ttttttatat
    16801 gctagaaaat tgatagcaag tacagatttt tatgaagctt tcgagcagcg ggtcattgaa
    16861 aaaatccaat ttgcagaaga attatccgga caaatggaga ggcagaagat caaggcactc
    16921 cacaataaat cggaggaact tccttcgttg gaacctgcac gtcagcttgc gtctattgat
    16981 gagatcgaac agcttcctta tcatgagttt atccaaacag cgagtaaact tattgtatcg
    17041 ccagaggtcc atattttagc aagggcaaaa ttactggaga cacttaggca attgaacgaa
    17101 tgccatcaag ttttctattt gacgattgaa gaaaagttag tcaaagtcat accaaaagat
    17161 ctgccaaagc cgcagcagca ggcaagttac cagcaattat gtgtattttc agatcgttac
    17221 gggaatgaag atgcgctgct tagttccgtg ttaaaagaag aattcacgtt gcaatctgca
    17281 attgtttatc ctgtctatga tacatacatc gaagacccga agcgctggtt tcaactgact
    17341 gtagaggcgt gtacaggaaa aacgatgtat gaaggtacag aagatgaaaa acaagatttt
    17401 ttaaaaaaaa gagaaaaaat tttgcagcaa atgatcttct tccattagat tttatcagtg
    17461 aaaaagataa aaatgttatg aaaaacaagg attctatcca ttcaatatgt ttacaatttg
    17521 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
    17581 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
    17641 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
    17701 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
    17761 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
    17821 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
    17881 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
    17941 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
    18001 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
    18061 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
    18121 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
    18181 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
    18241 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
    18301 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
    18361 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
    18421 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
    18481 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
    18541 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
    18601 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
    18661 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
    18721 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
    18781 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
    18841 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
    18901 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
    18961 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
    19021 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
    19081 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
    19141 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
    19201 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
    19261 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
    19321 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
    19381 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
    19441 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
    19501 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
    19561 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
    19621 cttaaattgc ttcaatctgc tgattacaat gtggaacttg cagaaaaagg gatcatctat
    19681 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
    19741 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagtgtg
    19801 ccacctcagg gcggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
    19861 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
    19921 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
    19981 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
    20041 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
    20101 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
    20161 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
    20221 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
    20281 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
    20341 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
    20401 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
    20461 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
    20521 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
    20581 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
    20641 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
    20701 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
    20761 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
    20821 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
    20881 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
    20941 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
    21001 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
    21061 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagtcttttt
    21121 ttgagcttaa cttacttttg tttcttgtct tttttctttt cattttgtgg ctgtggtttt
    21181 agaaatcgat cgatctcttc ttttggtgca actgtttttt ccggatcgac agcaaatgat
    21241 tcgaaaagtt tatctaaatt atctgtacgt tcaaatttca accccattgt aaacacctct
    21301 ttgtttcttt ttacttagtc tatcatgtaa tcgtccaaat tggttt
//
LOCUS       DANKBF010000052        20664 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000052 DANKBF010000000
VERSION     DANKBF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            168..362
                     /locus_tag="PWY89_001994"
     CDS             168..362
                     /locus_tag="PWY89_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067029.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="PWY89_001995"
     CDS             631..930
                     /locus_tag="PWY89_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067030.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="PWY89_001996"
     CDS             complement(1027..1461)
                     /locus_tag="PWY89_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2067031.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="PWY89_001997"
     CDS             complement(1693..2403)
                     /locus_tag="PWY89_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2067032.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="PWY89_001998"
     CDS             complement(2393..3253)
                     /locus_tag="PWY89_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2067033.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="PWY89_001999"
     CDS             complement(3258..3896)
                     /locus_tag="PWY89_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2067034.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="PWY89_002000"
     CDS             complement(3911..4210)
                     /locus_tag="PWY89_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2067035.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="PWY89_002001"
     CDS             complement(4253..5737)
                     /locus_tag="PWY89_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2067036.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="PWY89_002002"
     CDS             complement(5757..6218)
                     /locus_tag="PWY89_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2067037.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="PWY89_002003"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="PWY89_002003"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2067038.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="PWY89_002004"
     CDS             7521..8291
                     /locus_tag="PWY89_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2067039.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="PWY89_002005"
     CDS             complement(8564..9391)
                     /locus_tag="PWY89_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067040.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="PWY89_002006"
     CDS             9959..11194
                     /locus_tag="PWY89_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2067041.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDP
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="PWY89_002007"
     CDS             complement(12193..12453)
                     /locus_tag="PWY89_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2067042.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="PWY89_002008"
     CDS             complement(12480..13781)
                     /locus_tag="PWY89_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2067043.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="PWY89_002009"
     CDS             complement(14073..14531)
                     /locus_tag="PWY89_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2067044.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="PWY89_002010"
     CDS             complement(14544..15875)
                     /locus_tag="PWY89_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL2067045.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="PWY89_002011"
     CDS             complement(15886..17328)
                     /locus_tag="PWY89_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2067046.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="PWY89_002012"
     CDS             complement(17315..18079)
                     /locus_tag="PWY89_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL2067047.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="PWY89_002013"
     CDS             complement(18183..19058)
                     /locus_tag="PWY89_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2067048.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="PWY89_002014"
     tRNA            complement(19313..19397)
                     /locus_tag="PWY89_002014"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="PWY89_002015"
     tRNA            complement(19415..19486)
                     /locus_tag="PWY89_002015"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="PWY89_002016"
     tRNA            complement(19496..19568)
                     /locus_tag="PWY89_002016"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="PWY89_002017"
     tRNA            complement(19630..19714)
                     /locus_tag="PWY89_002017"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="PWY89_002018"
     tRNA            complement(19735..19805)
                     /locus_tag="PWY89_002018"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="PWY89_002019"
     tRNA            complement(19851..19922)
                     /locus_tag="PWY89_002019"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="PWY89_002020"
     tRNA            complement(19934..20006)
                     /locus_tag="PWY89_002020"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="PWY89_002021"
     tRNA            complement(20009..20082)
                     /locus_tag="PWY89_002021"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="PWY89_002022"
     tRNA            complement(20089..20169)
                     /locus_tag="PWY89_002022"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="PWY89_002023"
     tRNA            complement(20171..20246)
                     /locus_tag="PWY89_002023"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="PWY89_002024"
     tRNA            complement(20282..20354)
                     /locus_tag="PWY89_002024"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="PWY89_002025"
     tRNA            complement(20364..20436)
                     /locus_tag="PWY89_002025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="PWY89_002026"
     tRNA            complement(20452..20523)
                     /locus_tag="PWY89_002026"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="PWY89_002027"
     tRNA            complement(20538..20627)
                     /locus_tag="PWY89_002027"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
BASE COUNT         6532 a         4321 c         3273 g         6538 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatccagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaagc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaaaggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgag
//
LOCUS       DANKBF010000053        20627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000053 DANKBF010000000
VERSION     DANKBF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            352..1041
                     /locus_tag="PWY89_002028"
     CDS             352..1041
                     /locus_tag="PWY89_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2067049.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="PWY89_002029"
     CDS             1028..2236
                     /locus_tag="PWY89_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2067050.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="PWY89_002030"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="PWY89_002030"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2067051.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="PWY89_002031"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="PWY89_002031"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2067052.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="PWY89_002032"
     CDS             complement(6339..7034)
                     /locus_tag="PWY89_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL2067053.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="PWY89_002033"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="PWY89_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2067054.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="PWY89_002034"
     CDS             complement(8233..8424)
                     /locus_tag="PWY89_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL2067055.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="PWY89_002035"
     CDS             complement(8445..9335)
                     /locus_tag="PWY89_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL2067056.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="PWY89_002036"
     CDS             complement(9322..10089)
                     /locus_tag="PWY89_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2067057.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="PWY89_002037"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="PWY89_002037"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2067058.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="PWY89_002038"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="PWY89_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2067059.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="PWY89_002039"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="PWY89_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2067060.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="PWY89_002040"
     CDS             14660..15043
                     /locus_tag="PWY89_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067061.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="PWY89_002041"
     CDS             complement(15211..15969)
                     /locus_tag="PWY89_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL2067062.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="PWY89_002042"
     CDS             complement(15987..16805)
                     /locus_tag="PWY89_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2067063.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="PWY89_002043"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="PWY89_002043"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2067064.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="PWY89_002044"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="PWY89_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2067065.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="PWY89_002045"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="PWY89_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2067066.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="PWY89_002046"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="PWY89_002046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2067067.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5896 a         4212 c         3791 g         6728 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaatt
//
LOCUS       DANKBF010000054        20360 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000054 DANKBF010000000
VERSION     DANKBF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            291..1430
                     /locus_tag="PWY89_002047"
     CDS             291..1430
                     /locus_tag="PWY89_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL2067068.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="PWY89_002048"
     CDS             complement(1497..2072)
                     /locus_tag="PWY89_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2067069.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="PWY89_002049"
     CDS             complement(2077..3051)
                     /locus_tag="PWY89_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL2067070.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="PWY89_002050"
     CDS             3334..4302
                     /locus_tag="PWY89_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2067071.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="PWY89_002051"
     CDS             4415..4693
                     /locus_tag="PWY89_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2067072.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="PWY89_002052"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="PWY89_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2067073.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="PWY89_002053"
     CDS             5150..5905
                     /locus_tag="PWY89_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2067074.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="PWY89_002054"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="PWY89_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL2067075.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="PWY89_002055"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="PWY89_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2067076.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="PWY89_002056"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="PWY89_002056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2067077.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="PWY89_002057"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="PWY89_002057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL2067078.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="PWY89_002058"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="PWY89_002058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2067079.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="PWY89_002059"
     CDS             10642..11184
                     /locus_tag="PWY89_002059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2067080.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="PWY89_002060"
     CDS             11207..12763
                     /locus_tag="PWY89_002060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2067081.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="PWY89_002061"
     CDS             12777..13679
                     /locus_tag="PWY89_002061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2067082.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="PWY89_002062"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="PWY89_002062"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2067083.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="PWY89_002063"
     CDS             15122..15544
                     /locus_tag="PWY89_002063"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2067084.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="PWY89_002064"
     CDS             16013..16243
                     /locus_tag="PWY89_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL2067085.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="PWY89_002065"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="PWY89_002065"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2067086.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="PWY89_002066"
     CDS             17654..17830
                     /locus_tag="PWY89_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2067087.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="PWY89_002067"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="PWY89_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2067088.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="PWY89_002068"
     CDS             18325..18708
                     /locus_tag="PWY89_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2067089.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="PWY89_002069"
     CDS             18877..19761
                     /locus_tag="PWY89_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2067090.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="PWY89_002070"
     CDS             19875..20111
                     /locus_tag="PWY89_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL2067091.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="PWY89_002071"
     tRNA            20199..20279
                     /locus_tag="PWY89_002071"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="PWY89_002072"
     tRNA            20286..20357
                     /locus_tag="PWY89_002072"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DANKBF010000055        19762 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000055 DANKBF010000000
VERSION     DANKBF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            104..1147
                     /locus_tag="PWY89_002073"
     CDS             104..1147
                     /locus_tag="PWY89_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2067092.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1320..1544
                     /locus_tag="PWY89_002074"
     CDS             1320..1544
                     /locus_tag="PWY89_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067093.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1547..1804
                     /locus_tag="PWY89_002075"
     CDS             1547..1804
                     /locus_tag="PWY89_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067094.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            1906..2259
                     /locus_tag="PWY89_002076"
     CDS             1906..2259
                     /locus_tag="PWY89_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067095.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            2312..3106
                     /locus_tag="PWY89_002077"
     CDS             2312..3106
                     /locus_tag="PWY89_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067096.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3135..4253
                     /locus_tag="PWY89_002078"
     CDS             3135..4253
                     /locus_tag="PWY89_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067097.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4286..4606
                     /locus_tag="PWY89_002079"
     CDS             4286..4606
                     /locus_tag="PWY89_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067098.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            4861..5550
                     /locus_tag="PWY89_002080"
     CDS             4861..5550
                     /locus_tag="PWY89_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HDL2067099.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            5587..5754
                     /locus_tag="PWY89_002081"
     CDS             5587..5754
                     /locus_tag="PWY89_002081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067100.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            5774..7402
                     /locus_tag="PWY89_002082"
     CDS             5774..7402
                     /locus_tag="PWY89_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067101.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            7453..7824
                     /locus_tag="PWY89_002083"
     CDS             7453..7824
                     /locus_tag="PWY89_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067102.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            7909..8109
                     /locus_tag="PWY89_002084"
     CDS             7909..8109
                     /locus_tag="PWY89_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067103.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8243..9469
                     /locus_tag="PWY89_002085"
     CDS             8243..9469
                     /locus_tag="PWY89_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067104.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10066..10461
                     /locus_tag="PWY89_002086"
                     /pseudo
     CDS             10066..10461
                     /locus_tag="PWY89_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            10539..11578
                     /locus_tag="PWY89_002087"
                     /pseudo
     CDS             10539..11578
                     /locus_tag="PWY89_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            11591..12529
                     /locus_tag="PWY89_002088"
                     /pseudo
     CDS             11591..12529
                     /locus_tag="PWY89_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            12522..12869
                     /locus_tag="PWY89_002089"
     CDS             12522..12869
                     /locus_tag="PWY89_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067105.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            13184..14041
                     /locus_tag="PWY89_002090"
     CDS             13184..14041
                     /locus_tag="PWY89_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2067106.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            14061..14621
                     /locus_tag="PWY89_002091"
     CDS             14061..14621
                     /locus_tag="PWY89_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HDL2067107.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            15510..16277
                     /locus_tag="PWY89_002092"
     CDS             15510..16277
                     /locus_tag="PWY89_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HDL2067108.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            16303..16848
                     /locus_tag="PWY89_002093"
     CDS             16303..16848
                     /locus_tag="PWY89_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067109.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            16849..17415
                     /locus_tag="PWY89_002094"
     CDS             16849..17415
                     /locus_tag="PWY89_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067110.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            17430..17678
                     /locus_tag="PWY89_002095"
     CDS             17430..17678
                     /locus_tag="PWY89_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067111.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            17697..17936
                     /locus_tag="PWY89_002096"
     CDS             17697..17936
                     /locus_tag="PWY89_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067112.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            17953..18537
                     /locus_tag="PWY89_002097"
     CDS             17953..18537
                     /locus_tag="PWY89_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067113.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            18644..18925
                     /locus_tag="PWY89_002098"
     CDS             18644..18925
                     /locus_tag="PWY89_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2067114.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            18915..19256
                     /locus_tag="PWY89_002099"
     CDS             18915..19256
                     /locus_tag="PWY89_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2067115.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         7491 a         2615 c         4037 g         5619 t
ORIGIN      
        1 caacaacgat tatgatctta aaacaatcgt ttatgtaatt ggcaatctta cgaataattt
       61 tcatgcaata atgacaataa aaaatgaaaa ggggtaaaat caaatgaaaa aatttttagt
      121 aggaattcta ttcacaatgg ggctagtatt attatcacct tcagtttcag cagaaagctc
      181 tatttccatg tatcgtatgt ataatcccaa tagcggagag catctataca ctagaagcgt
      241 tggagaaaga gacaatctga aacgttatgg atggcattac gaaggtgtcg catggcaagc
      301 tccaacaagt ggtactcctg tttatcgttt gtataatcgg tataacggtg aacattttta
      361 cacagttgat gccggagaaa gagataagat ttcacacaca ggatggacgt acgaaggtgt
      421 tgcgttctat tcctacaacg gagcaaatag agttcctgta acacgtttat ataacaaata
      481 cgtgaattgg catcactaca cgttagatca aaatgaaaag aacgtaatta gtaaacaagg
      541 ttggaagatt gaaggtacag gctggtatgc aatgccaaac agcgtaccaa atcctacacc
      601 tgctccaagc actgaaaaag tgttgaacgt tcctgttgta tggcaaggaa atacaatgtt
      661 atgcgaagga gcttctttat tagaaggttt acactacaaa ggtgtagcca caaatcaaag
      721 cttaaccagt tttgtaaact atatgccaaa agctaatgac aataatcctt atcatggtta
      781 ctcaggagag tggcgtcata acgttaatgg aacgtatcaa ggcatgatgg ctgacccagt
      841 agttaaatgg gcaaaaaacg ttggcggtaa tgccgtcaat atcactggtt caggaacgaa
      901 tggaatcaag aatgaaattc gcaatggtaa tccggttgta acttggatta cttataacta
      961 tgctacgcca gagttcaaac aaatgccttg gggtcgtgca gtatggaatg gacatgtagt
     1021 tctcgtggat ggcttcagaa atggtgcata ccatgttgtt gaccctgtat ttggcgcaaa
     1081 atggatcaat gcaagcacgt atgagagagc atttaatgtg actggaatgg cggttgctgt
     1141 acgttaattg atagaattat caactaaatg aaataagagc ttaattactg aaatggtaaa
     1201 taggctctta tttttttaga ggaaattttt gtaatacgaa tctataacaa aaaatattga
     1261 atgtagtaca caaaagtggt atattaaaat cgtagagatt atgcgaagag gtgattttga
     1321 tgaaccaaag aagaagaaaa aggttagaag aaataagcga gcgactttcc gagctaaagg
     1381 atgagttaga agtaattcaa gaagaagagt acaaagaaaa tatacctaaa aatctacagg
     1441 gatcagaaag atatgaaaaa gcagaagaat ccgttgatca acttgaagaa gctgttgatg
     1501 gtttatccga catattagaa ttgattgaag atatagctag ctagagatga ttaatgaaga
     1561 tttcaggagt gagaataaaa tgattgatat ttcactgata gagaagttac ttttctcaaa
     1621 taagtacacc gcaaaagagc tggctacaag atggcaaata tccgaatcag ctattaaagc
     1681 ctatcgtcaa aataagggaa gcgtaggatg gcgtgattgg aaagggatca gcattggaaa
     1741 agcagaagac ataatgaaac gtgaaggact tcgatcctat accacttctg aaaaagacaa
     1801 ataacttaat gtttgaaaag ataaaatatc tgtcttaaag ctatgtcaaa ttcaaatggt
     1861 gaatgtttgc taaatataat gcaaaaaaag gaagggaaat attaaatgat aagcgaaaat
     1921 ttgtcttatg agaataataa acatctagtt aaagaaaata agaaagtaga agattataag
     1981 aacatgtata tagaaatgaa aaaatcacca ctgtgtattc aatataaaaa tgataattgg
     2041 aagcaagtag tcgaatttgt aaaacctcat agtatacgaa gagtacgaag ggggaagaaa
     2101 aaaagtgaac ttgtaaatag cggaagagcg gtaattagag ttataaccaa agattatcca
     2161 gttgaatatg gagattatat tttaaggtta agttatcatg cttttgttgt gttgaaagaa
     2221 gagcatttca acgagttttt caatttaaaa agtaagtaat catatttttt tgtagacgtg
     2281 tataaagtta actaaattgc gaggtgatga catggtagca aaaaagaata ctctaaaaga
     2341 agcttctgaa aaatatcaag atatgaaaag acgaggatta ataccgaaag acaaaaaagg
     2401 tatatggaga gaatttgtta aggaagtcag tagtgatcga agtggcggtg actttgttgg
     2461 acgatataca gcaaaaggct atagcaaacg attaaggatc actgttgaat atggaacgaa
     2521 tgaatcagct acagagctga agcaaaggta cttagataca cgaagggaag cttcaaaaaa
     2581 atataatgat ttcaagagca gaggtctgat tcctaaaaag aaaaagggaa tttggcgtga
     2641 gtttttcaag gaagaagata gcaatcggaa taagggagac ttcattggac gatacacagc
     2701 aaaaggctac actaagaatt tagggatcaa ggttgaatac ggaactggtg aaagttcatg
     2761 ggaattgaga aatcgctatt taaatacatt gttagagaaa tataaagcag aattattgga
     2821 gttgttcgat gttaatgatt ttatcgagat ggctgaaaag tatggtcatg aagaaggttt
     2881 tatgatcaat gacattatgg ttattgataa ttttacggat ttattagcac ttgcgaaaaa
     2941 agcaagggat ttaagagaaa cgcagatgta tctattcaga aataaaaacg gaagaaaccg
     3001 gagaactgaa tcgagtgaga ttgaagatta ctacgatgcc tttacacacg atctgggagc
     3061 ctttgtagac gagataagtg gtgtaatgaa caaatatagg gtctagaata aaaaagccgg
     3121 agatgagcag ataaatggga aacaatagca tatacacacc tgaaatgcag aataaaatta
     3181 aaagacttgt cggattaggg tggatgacaa gaggaaaatt agattctttt agtacgatca
     3241 aatatctttt gatggacatt taccgtcaga acaagcaaac aggaatacat tttttgggct
     3301 atgataagcc ggaagatgca cctttctttg ttccaagttc agcctataga gccagtacct
     3361 ccggtagcat gtataacttg gatttgtttg aggaaggagc aaagatgatc gaatatgatt
     3421 tcaacgaagc ctattcgagg atcatgagaa attatccttt gccgtcaaac acttatttga
     3481 aagcaaaata taatacagat caagctattt tgagagcgat aaatagacca aaagtaagac
     3541 cgtatgaaaa tttaagaacg tatatgttct ttttggttaa ttttgaagga acagccaaag
     3601 aaggaacata tgctaatcca aaatcacagt cgatgtttgc ggattatggc gaaaatccca
     3661 aaacagctaa tgagcctatt tggttatcgg agattgagct aacacttatc cttgattatt
     3721 atgatattaa agagatggaa gtcctagata gccacacatt tttgacgaaa aaaggattac
     3781 tcaaagaata ttttgaaaga atcgagccat taaaaactga tcctgatacg ttgaagcttt
     3841 ataaaaatct tagaacgaaa gtatttggtg ctatcggaca aaaatcatta agagaaggag
     3901 aaacaagcac cttcgataaa tacagtatgt ataatcgagc gtattcctcc atggtagcag
     3961 gcgtatttag agatcgtatg gttagatatg aacagaaata cgttcatagc gcatatgggc
     4021 tattaaaaat tcggactgat ggattgtatt tcagatccga agtaccagag tttgaagagc
     4081 tgtataaact tggagtagtt aagaaaaaag ttcatacaat atccaaagaa gaagtcgaaa
     4141 aagctttaga atttaaaaac aaaggatttg aaaataaggg ctcctttggg aaatctaaag
     4201 atgaagttaa gggtgttgtg atagacgatg atttcgagtt tgattttgac taaatactct
     4261 ttggaaaaga tggtaggagg taaatatgaa gaagaaaatt gtggaaggaa atgtaggtgt
     4321 tattattcta aaagattcaa ctgactatgc taaatcaatg gcactaaaag acgaaaaagt
     4381 tgcacgcatg gataaagatt tagttgaatt tctgatggat gatacagaaa gattaaaagg
     4441 ggatcagttt ttgagtttaa tgaaagacaa aggcttaaac acatatggtt tatttgatta
     4501 taagaaatta gctatagagt gggtaaaaga aggagaaata ttttttattg aagaatggga
     4561 aggtactgag aacttagttc ttcatcagta tcttttgaaa gcttaatcta agtaaaacag
     4621 ttctgactag ttatgcttgt cagaatttca aaggaagttt tccataacca tcatcattca
     4681 aaaataaagg atttgaaaat aagggttcct ttgggaaatc taaagatgaa gttaagggtg
     4741 ttgtgataga cgatgatttc aagtttgatt ttgactagat ttttttgaaa ggtttaagag
     4801 taaacaaggt aagaactaag atcatgaaga ttgagagtaa agaagataga ggacgaatgt
     4861 atgagaacac actatattta tttagaagat aaaattgtag atgaaattcc ggcaaaaagt
     4921 cgagaacaag ctagaaaatt atttattgaa aaatacaaag agatcaatgg agatagtgaa
     4981 gttccaaaag tggtcgtaac agttagaaaa aggcaaacaa aaaagagaag tacgtttcat
     5041 gtcagccctg atatatataa tcttatagag cgacatagaa gtgaagataa tatttctatc
     5101 tctcaatata ttgctacagt tattgatgaa ttgatttcag gaaagtatga ctatgctttt
     5161 gattttgagg gtgaaaagga ttacaaaagt gttgctgtct ttcaatctga ttttgagaag
     5221 ctaaaaaact atgctggcag gagaaatgtt aattttgcaa gactactaga agagttctac
     5281 tccgaagagt tgggagtagg aaatgaatac aagagagaaa cttatgcgaa attgtctcat
     5341 aagttatccc aagcgcttaa agaagtgaca gaagttaaag cggaactaga tgctaaactt
     5401 gaagaagtga aaaaatatca aacagagagg aagaacaaca tgaagaatca agtggaattt
     5461 gacgaacaga aagaatttga aaaagcaaga aaacgctata acaaaattat gaatgaactt
     5521 agctatgaac acttaacagt tcatgaataa ttgagtgagg gtacagaaaa atatacgatc
     5581 gcagatatgg ttaatgaagt ggaaactctt ttagcttctt tctttgaaaa tggtcacgta
     5641 aacgatgaat ggcgaaaaga agaacctaaa agggttaacg cttatattgc tcgattaaaa
     5701 aggtttattg ataaatataa ggagaatagt gacaaatttt ctaaagataa ataaggagag
     5761 agtgtgatta catttgccag aaatcgaagg aatattttcg ccaaaaatag gtaaaaaaca
     5821 gttagatttc agcagttttc cgagcgtaaa tagagattat aatctaggtg atgaggtaca
     5881 gatactaaat attgatttca aagaactaga cttcgctgtt gctgtttcca acaaacccaa
     5941 ggcattggaa cgagtgaaaa aaatatacca agagaatgat caaaatatca aaaatatgat
     6001 caaaaagtat agacgagata attcaaaagt tgaagacgtt ttattcaacg gtacggcaaa
     6061 acatgatttg aatgtttata tggcattagg agtggctttg tactgtaatt caattaatca
     6121 gacagatatt ttggttaata tcgtgaggaa tagctatcaa aaaatattaa aaataagtag
     6181 cgaaacaaac ccttattatg caatgaaata tcttggggat aatttattaa atgataaaaa
     6241 attgatgcac aacactgaag agctgttatc taatttggct gcgattaact ttctattcaa
     6301 aacaggttct gaagattatt ttatagataa cgtactaaaa ggtgcttacg gaacagcatt
     6361 ttatccgctt tacaatatga aacagcataa agccgaacaa aaggataaca aacttgaaat
     6421 taaaaagatg aaagaactat taggtatagg atttgtaaaa cccaattcaa aagtcactat
     6481 tgcttcgtta gcaactacta acaatgatgg gaaaatagta aatgggtttg acgattcaga
     6541 tatagatata ttgtcaaaat catatttttc tttgtatgga gattttgcag atttgatggg
     6601 tattgatagt tacttagtca ataattttga gatcaagggt agcgagctga atgatattgt
     6661 gtatttcgtt ttaatctctc tatatgcaat tgatcagaat accgagtacc gacaaattga
     6721 ctatttgcta gttttttata gtttgttata tcagttcgtt ttaactcggt tgtttaaaaa
     6781 tgctaaggaa gaattgatcc tgaattctga ttatgaaata gcaaaacaaa ttgcaagtat
     6841 tagaaaagaa aacaaatctg aagtaatcaa attaagaaaa gaattaagta ttgcgagtaa
     6901 agaaaacggt cggttagaat ctttagtcaa atcggcaaaa aaagaagagg aacttttgaa
     6961 gcaagagatt aaagaattaa aaggaatcag aaaagaactt gaacagctga ggtttgaaaa
     7021 taaagtatta aagcaattgc aggagcaaga acatagacaa tcattaaatg gtaaaactga
     7081 agagccttca ctgtctgaaa tgatagctta tctaaaacat aaaaacattc ttttagtggg
     7141 aggacatgta aattttcaaa ataaattaac tgaactatta ccaaatatta aaatgtttga
     7201 tgtaagcgaa aataatggaa agatacttaa aaatttgaat acgtgtgatg cgatttttct
     7261 atatacagat tacttgaatc atggtctata ttatcgtgtt atgagtgaaa tttctaataa
     7321 agataagata ttttatttgg ataatcatgt gaatatgaac ttaacaatta aacgtattta
     7381 cgatctttta tcacttatat aaagagagaa gtgataaata ggacacaaaa tttggaaagt
     7441 aggtaagtca ctatgagaga actaacttta tcttctaagg aattagaaaa gaaaactgct
     7501 ttattaaacg aaatatttag tgctgattgt ataatccaaa gtttaaaaga aagttcattt
     7561 tcaaaactaa cagatcagga aaaaatttct tatatgctaa gtttagataa tctaggaact
     7621 aaatatgatg aatcagcaag tagcgataag cttttgattg aattagtatc aagtcttagt
     7681 agttttggaa aaatgaaaag atttaatgtg atattagaaa tttttgcttg gttaatccct
     7741 tcatttattt ggttttttta tattttcata gttgaatctg agttaatagc caaggtctct
     7801 cttggactgt taattccgat ataaataggt tcagcaattt actttaataa cttgtataca
     7861 cctattcaaa taaaaaagca gttattacat gaaggattaa gtggtaatat gttaaaaaga
     7921 attgcatggg ataaagataa taaaacagat attgagctta aagaatataa atacaattat
     7981 ttctcgagat gtatagatag aaagttactg tttaaaaaag aaggattaga atttagtttt
     8041 gatgaagatg aatttgaaaa attagtgatg gactggcgta agaatttact ggatacggta
     8101 caattctaaa agcaattgag gtcgaaattc cgatacttga atcatgatct atattatcgt
     8161 gttatgagtg aaatttctaa taaagataag atatttcatt tggacagtca tgtgaatatg
     8221 agcttaacaa ttaaacgtat ttatgatctt ttatatgttt ataaacatta atgaggtgct
     8281 aaaattgaca acaataacta tgacaaaaaa aatagaagta tatgttgatg gatcacaaga
     8341 agaaagggat tccttttttg attttttttg gaaaaatagt agagaatatg taaaagcgtt
     8401 gaatctggta tataaacagc taatgtttga agagttgata aaagatgcaa ttaaagaaat
     8461 ggatcaaggt tatgctgaga gagaagaaag tattcaaaat cggattgaaa agttagcata
     8521 tactgatcca atagatcaaa aaaaacttga taaagcaaaa gctgaattat ctaaattgag
     8581 aaatgaaaaa aataaagaag cgagagaaat attaacgcaa gcgatagggt tgaagagtca
     8641 aacgagaaca cgagatgtag caaaaaaatt agactttgac tttggagatt ttgttgattc
     8701 cgcaaatatg aaagctagta aagattttgg caatgatttt atcggaattg tgaccggaca
     8761 aagaacgcct agaatatata aaaatagaaa caaatatgga ttattacctt ttagaggtcg
     8821 agatattaaa atccataaag atgataagtt ttatatttat ttacctaaag gctttaggtt
     8881 taaagtgaat ttaggtagca agccaagaaa agcaagagat gttgcttcag ttttacaaca
     8941 cattatggat ggcgattaca aattaaatca aagtaatctt atttttgatg gaagaaaatt
     9001 attttttaat ctaacaattt cttttgaaaa aagctataat gaaatggaat tcaagaaagg
     9061 taatgaattg atcgtaaact ttgcagattt gtgtagcggt gaatgtttaa ttaatgataa
     9121 atctgtaatg tcttttggtg atagccaata tataagaaac ttcaaaagta aaattttagc
     9181 aatgaaatca aaagaacaaa gccgaggagt cttttctaag ggcggtcatg gtcgaaaaag
     9241 aaaattagat caagatcgat gggaagcaat aaagaaaaga gaaagtaatt ttgttaaaac
     9301 ttataataat caatgttctg caaaaatcgt taaaactgct attcgcaata aagctcaaat
     9361 tattcgtatc attgaaccag ataaggctta tggcgaatgg gcttactatc aattgaaaga
     9421 acaactagta aacaaaggaa aaagatatgg gatagaagtc agaagttagt aaattaaaca
     9481 atcgttgatt tggcaacgaa atagtgaggg aaaatccact cactgaaggg taaactccgt
     9541 caagttgtgt gaccagacgg aaggacgaat gttgttgagg ttttagaacc atataaaaca
     9601 gcatgtttct caaattttta ggataaggga aattgctagc agtttgtttt agaaccatat
     9661 aaaacagcat gtttctcaaa tgagtacttt aaattaattg atggcgttcc tgctttagaa
     9721 ccatataaaa caacatggat ttaaaacaag aagtaagtca gttctccttt tactttagta
     9781 aggaaaaata aagtattctc aataaaactt aaaataagca aacctttgtt ttaaaatgat
     9841 tgtcaagtta aaaaaatctg ggatcatata aaatagtata gttctcaaat atgattagaa
     9901 accctattaa agagcaatat taagccaact tacttaaatc gtttaattaa gaacaataat
     9961 ctcttttagt cagactttta gtctgacttt tttgtatttc caaaatttat tctttacaaa
    10021 aacgaacata cgtttgtata attacttgtg aatgggggaa taaaaatgtt tgattacagt
    10081 agagaaccgg tgaatgacta ttttttaatc gacatgaagt ctttctatgc tagcgtggaa
    10141 tgtattgaac gaaatttaga cccaatgact acaccgttaa ttgtcatgag tagatcggac
    10201 aacactggtt caggtctgac tctagcttct tcacctactg caaagaaaaa atatcatatt
    10261 actaatgtta gtagagctac tgatttacct tatccattac caaaagaact tcatattgtt
    10321 ccaccacgaa tgaacttata tataaaaaaa aatatggaag ttaatcagat ttttagaaag
    10381 tatgtcgcag atgaagattt attgatttat tcgattgatg aatcaatttt aaaagtgaca
    10441 aaatccttaa atttatttac tatataaatt gagttaaatt gtattcgttt ttcctcctat
    10501 aggcgtatac taaagaaaac acttgaggag tggggaacat ggaaacagaa cgccaaatcc
    10561 gtgaattgga aaaagcgatg aatacgacag acaaccgccg gttgtacgag cgtttcctgg
    10621 cagtcaagtt ggttcttgaa ggccacaccc ggaaagaagc cgggcacacc atcggccgcg
    10681 acgagcatac tgtcagccac tatgtgaaaa actactgcca taatggaatc caaggtttag
    10741 tctccaaaaa acaaagcgga cgaccttcac ggctgacaga tgaacaggaa gcggaactgg
    10801 tccaaatcgt cgcctaccac actccggagg aagtaggttt caaatcccgt gccaattgga
    10861 cattggcgat tgtgtgtgac ctgattctcc gggaatggag ttatggatat acgcaaaaag
    10921 gcgtggcgga cctccttcac cggctaggac taagctggac ccgtccgacg tataccctca
    10981 aaaaagcgga ttccaagaaa caacaggtat ttttggaaga aaccttcccg agcctaaaaa
    11041 aaaattgctg aatagcgata ttgaccacct cctattcgag gacgaaacca tgatccggga
    11101 ttaccaagcc attggctgca cctggtttct ccgcggccac cagcgaaaaa tccccactta
    11161 tggacaacac aaaggggtaa aattgatagg caccttggat tatgaaaccg gtcggatcat
    11221 ttgtacagaa gaaggggcat acgacgcagc ggccttcctc cgttttctgg agcggacgtt
    11281 gcgggaatac cccaccggaa aaatcgtgat ggtattggat aatgccctta tccaccacgc
    11341 caagctgatc cagtcttttc ttgaagaaaa cgctaggcgc ctggcactca tcttcctccc
    11401 tccctacagc cctcagttga acctaatgga aggcgtttgg aagtggctca aggaatcggt
    11461 catcaacaat gttttttttg accacgtcca gaaaatcaaa cagtcggtcc gcggtttctt
    11521 agccgatgta aacgagcgtc cccttgaggt gattgaccga ctgtgcgtgc ggatgtaaag
    11581 tatttaattc aacctatata gttcaggaac aagaagcgag agaagaagag aattagctaa
    11641 aataatcaaa gatgagattc agaaagaaac aggtctaata gcaacagtcg gaataggaga
    11701 taacccatta ttagctaaac ttgcactgga caatgaagcg aagagtagta aagaatttat
    11761 agctgagtgg acttatgaga ctgtacctga aaaaatatgg aaaataaacg agatgactga
    11821 cttttggggc attggatcac gcatgaaaaa aagattgaat catatgggaa ttatgagtat
    11881 agaagattta gcaaattgga atccctacac aattaaaagt cagttaggtg taatcggact
    11941 ccagttatat ttccatacca atggaattga tcgtacggat attgctattc caccagaacc
    12001 aaccaaagaa aagtcttatg ggaattcaca agttctacca aaagattata cgaggagaga
    12061 agaagtagaa ctagtagtca aggagatggc agagcaagta gccattcgaa ttagacaaca
    12121 caattgcaaa actggttgtg tacgcttagc aattggtacg tcaattttgg aagaaaaatc
    12181 aggtttctcg caccagatga aaataccaat tacagataat acgaaagaat tacaaaatta
    12241 ttgtcttttt ttattcaata agttttatga ggggcaggaa gtacgacata taggagtaac
    12301 ttatagcaga ttagtataca cagattccct acaattagat ttatttagtg atccaagaaa
    12361 aaagattgct gaagaaaatt tggataggat tattgataag attagacaaa aatatggatt
    12421 tacagctatt atacatgctt catctatgtt agaaggtgca agaagtatta cgagaagcac
    12481 tttaatagga ggacacgcag gaggattagg aggtttgcat aatgattgaa tataacgata
    12541 gaggacgttt aaaatggtcg ggattttatt tgtctgatca tactgaaaga atcaatgcag
    12601 acaatgtaca acgtgctaat cataatttgg caaaagaaca aatgacgact gaagagattg
    12661 cgaaaatact gagaactgct gtcataaaaa atagacccgt ctgcattcaa aaagaggaac
    12721 gaaacgctga aggttactat ccagatgatg tagtaggaaa aattattgga tatgacgagc
    12781 ttggtgtatt tgtggaagaa gaaaaagttc actatgatga aattaggaat gtttctttct
    12841 cggaattatt aaaatggagt cagatatgaa cattattaaa aaatacgaat tcggttatat
    12901 tacgtatgaa cagctaatag aggaattatg gggatatggt caacaattaa ttaatgaagt
    12961 ggggattgat tgcttttgtt tctatattga atccggagca ggataccata agaacagata
    13021 ctatatattt ccatatccac caaaatagtc tcttagatat ttttaaattt tctccaaata
    13081 ctttcaatta ttgtgaaata tttcccttta attaatcaaa ttttttataa aagtaatata
    13141 taaaataaat tgcttgaagt attatattta gatatcataa tttatggaag aaaaattaat
    13201 taaaaaggat gagattataa tggggttact ggttccgaga gctaatactt atactatacc
    13261 tttaacgaaa cgccaattaa ttgctaaaag atatcaaagg attactagag ccatcaatag
    13321 agagttttgg aattctgaaa gtgatactgc tcacagttta tacgtaggtt cttatgggag
    13381 aggaacggca attagtacta gtgatataga tataatagtt gaattaccga tggcagaatt
    13441 tgacagattc aaaaattatt tatcaaatgg cccgtctaag ttattacaag ttataaaaaa
    13501 cgcatttcag gaaatactac caaatagtga tattagagct gatggacaag ttgttaaaat
    13561 aaatttccat gatggaatca aatttgaaat agttccagct tttaatgaaa aagattattg
    13621 gggtgagtca aaagggttta tttatccaga ttcaaacatg ggagggaact ggaaagctac
    13681 aaatcccaaa aaagagcaag aagccatgaa attaaagaat acaaaaagta ataatttatt
    13741 atatgctacg tgtaaacatt ttcgacatgt acgagataca gaatttacga gttatcatct
    13801 ttcaggaata gtaattgaca gtttcgtata tgaggctatg ggaaattgga aattcgttga
    13861 aaataacagt gggggtcaaa acattagttc tgtatcttat gagacagctt tattagaata
    13921 ctataactca cataaagtta tgggcggttt aaatttatac tctcctggaa gcaatcaatt
    13981 tgttaattct gatagtagta tcatatgttt agaaaaagta ttaaaaaaaa tagctttata
    14041 aagttttatg gaggaacaac atgggagacg aaaaaaagcg cttggaagtt caattacgtg
    14101 aagcatatgg acgagtaacg tatacttaca ccactcattt aaagcaagcc gataatatta
    14161 tttctgacaa taaaatttta aggtgtcttc aaattggttt gtcagccctt actactggag
    14221 gggtacttag taccattatt actgataata gtgagttaac ctttgttgct ggattatgtt
    14281 caacaatttc tcttggatta aacttatact ttaagaattt tgatcttgct gaagaagcta
    14341 aaaatcatca aattgttgcc gataaattat ggttgatcag agaagaatac gtaagcttac
    14401 taacagattt aaatcgttta agtgttgaag atattgtgaa aaaaagagat caattaatga
    14461 acgaaactca tgaagcatat aaaaatgccc ctaaaacaaa tagtaaaagc tatgaatcag
    14521 ctcagaaagc attaaaatct gaagaagagc aattttttac agaagaggaa ttggataaga
    14581 tgttaccaga gcatcttaga aaaaagagtt ctaaatattg aactcttttt ttatgcttaa
    14641 aattctcttg taatttttct attcatctat atttaagaag aaatatgatg atagaaacat
    14701 ctcctaaagc caccgcaagc cgttctaagc atagcttagc ccgacattgc tagatatgct
    14761 catctcacca tgaacgtttc ctaaagccac cgcaagccgt cctagccatt gcctagtccg
    14821 acatcgctag attagtgcat ctcgccataa aagttttcta aagccaccgc aagccgttct
    14881 aagcatagct tagcccgaca ttgctagata tgctcatctc accatgaacg tttcctaaag
    14941 ccaccgcaaa ccgtcctagc catcgcctag tccgattttg cttgatcggt tccatttctg
    15001 tttatttagt gggtcaacac cgtaatctta aatagccatc gcctatttaa gattactagg
    15061 tctgctttga aggtaaaaag tctttcattc ttcaagactt ttaggctttc aagctacgcc
    15121 tgaaagcatt gaaattcatc gctctttatt tggttttgcc atttataatt ccttacgccc
    15181 attatctttt tcgcctgtca taccttgtca gaagtgttta gagcttatct catctttgga
    15241 ttaatacaag tggcgtaaat ttttttctaa acgcaagcgt tacgaaaata atttacttaa
    15301 acacttgtat taatccggca gataagaacg cattctaatc actcccgaca aggcaagaca
    15361 ggcgaaaaag ataaataaat gaacgaaagg aagaaacata aaatgcaaaa ccaaatagcg
    15421 atgaagaaaa aaccacctcc ctctaaaaca atccaaacaa aaaagaattt tgaaaaacca
    15481 aaaatcaaaa acagaaagaa gcgttcaaca tgagagatag aattttagaa gttaatcaag
    15541 aaatggtaaa caagttaaca aatatcagtg ggaactatga aaagtacgat ggtactttac
    15601 ttgataacta tatttttcac gatacacaaa atatcagaat tggcagaagt aaaccgagag
    15661 cgttcattct catcaaagag aaatatttga atgaatggaa tagcacctat gaaatggtta
    15721 tgactgataa ctttgaaaag gtattggatt tcagaaatgc tttgttgggt tctgatatgg
    15781 gaaatagcag tcttgaatct attgaagttg aaaacaagac cgagaatgaa ggtgaaggat
    15841 tgattaagaa tgtcttgaaa atcgaacaaa aatatatcag tgaagataga agtttttata
    15901 gagtcacaag ctcggttggt ctagcaaact ttgctactaa cttaattgga aacaatgcta
    15961 gtgaatcttt gctagtgatg tgtctgaata caaagaatga ggtcattgct tatagcgaag
    16021 ttttcaaagg tggaattaat caatcagtag cgataccaag agagattttt caactagctt
    16081 tgctaaacaa ttcagcaaaa atcgcagtat cacataatca tccgagcgga tatgaatttc
    16141 caagtgaaaa tgacaaaagt tttactacga atttgaaaaa cgcaggaaaa acaattggta
    16201 ttgaattgtt agaccatatc atcgtaacaa gtaaaaacac aagctattac tcatttcaag
    16261 aaaatggttc tttataaaaa ttagagaaaa gaggaaaaca agatgggcga aagaacacaa
    16321 gcgttaatta cagtcaaata tggtaacagc aaaacgaaag caagcgtgtc gattcattat
    16381 cagtgggggt atggtcgata tatgctgatg gacgtattga atcaaggaat ttatttaatc
    16441 aataaagcat acgacttacg agataaatca agagaagaat tagcaaaagg aatctttgat
    16501 agaagtacag gagttatcaa ttttgtcacc tatgaatctg gagtgagtgc ttatgatgaa
    16561 acagggtttg gtgggcgcta tgaaccttat caagaaacga ttgaagatgt gatgaagttt
    16621 tcagataaca atgatggcta tgctcattta gagatggtgt tcgatgaaaa tggatttagt
    16681 ctagatgata aaaccagatt gcgattgttt gattttgagg gtcaacagat aggactaaac
    16741 aagttttgtg aaagcggaaa agagtttgct acgaaagatt ttattaaggg atacagatta
    16801 ttgctagaaa attatggaat aaacctagaa gaagagagcg tgaaataagt gtttgaaaaa
    16861 agaaaagacc agttccctaa tttagaagcg aaaagtcaaa aagtgattga tggtgaaatg
    16921 attgaattat tccaaaaaag ttatggaacc atagaaacta ccgcaagttg caaatactac
    16981 tttcaaggaa agagaaacac gtttaaaggc tttgccgatt ctgatgaaga attggcaaag
    17041 aaaatcattg attggcttgt cagcagtgaa cacgtagcaa gagaagatac cgttagaaca
    17101 acgattaatg gggaagtcct aacaggtact agatgtgttt gttgtaatca tgaatttgta
    17161 gaagatgaag gttgctaccg aatcaatgaa tatggtgatt ttatttgcca gagttgccac
    17221 gagatagaat ttccaaccga aagaagttgg tttgaataca tgctacaaga ttatgagatg
    17281 gaaacagaaa atggtgaagt agttttttgt accaaagagg aattgagtaa gagaagcgat
    17341 gatgaaattg aagattggtg tgtgtggtta gtagggacag atgattgcgt tgcatattac
    17401 acaacagcag aataggagtg tgaatcagca tggaaggaat ggattttata gatcatgaag
    17461 atttgattga ttttggttat acatggaaag gaatggtagg tattagcaga agtcttgcta
    17521 atgcctttta cgaaagaaat tatgcagtct atgtccttta tgatgacaat acagaaagcc
    17581 ttgttgatga agagtataaa ttggatttag agaatgtttt atatgggata gaaaaagaag
    17641 atttagcaaa atatattttt tcatggttgg gtcaataaac gaaagagggt agcaacatga
    17701 caaaattcaa aagtaaaggt atgcaatggt gttcttatta ttatgttggg aatgatgaag
    17761 agcttttgag aaaagccaaa caaaaaggtt atccagataa gatgttaaaa cagattggct
    17821 attacatgcc agaagagaga atagaaaaag aatcttgtaa ggttcttttt gatagtgaac
    17881 agctattagt tttgttaact caaaataaat tgatttgtca atttgaaaat aattaatatg
    17941 gggtgaagaa ccatgaatga gaataagatg gtattaagtg aatacgcaag attgagaacc
    18001 attaattggt tgaatggtat gcaaagtagt ggcgcttttg atggatttga tgaggagaat
    18061 atggactttg aaagtttatc gaatgaggat attagatatt attttatcga atggtatttt
    18121 acagaaccag atgaagaggt ggaagatgaa ccagaattaa ttttagaacg tcaaagaatt
    18181 ttattcagtg aacaagatag taagaggttg tatggtttat atgacttaca actgaaagga
    18241 ttaaaagcgc agtatgttgc tgatagttac gatgatatgg tagaacaggg ggcagatagg
    18301 gttgtcactt attcagaaga tgatgaagag gaatgggaaa actattcaga tgatgagata
    18361 tgctttaatg atgggtatat gcctattgaa atcaccgaag aagaatatga taaagtgtcg
    18421 gatagcgatg agattggtat gtgtacggtt gtaagtggat tggatataga tgtccaaaga
    18481 gtagaggttg cataccagaa gatgaaagaa cgtataaaaa gtaaagtaga atcttgattt
    18541 gattggattg tttgcaaagt agctaattaa gagtataata taatcataaa tgtagttaat
    18601 ggatataata atgagtatat tatactcaaa agaggtgaac gaaatgaagg taaaaaatcc
    18661 aaaagaaaaa agcagaattc aagtcggaat agacaaggat ttaaaagaaa acgcagaaat
    18721 gatattggaa gagctagggt taaatccaac aacagcaatc acaatccttt ataagcaagt
    18781 ggtcgctaga ggtgagtttc cagtagagat aaagctaagc gaagaggaaa agcaaggaat
    18841 cagattgcaa cagctaacta tggatatgcc agttgatgtt ctagatacag atgaaaagtt
    18901 agaagagtgg ttcaatgaag cctaatgaga tagtaacggt ctatgttgca tttacagata
    18961 aaaatggtgg taaaagaaga cctattttgg tggttagtga taaagaagat agagtagaat
    19021 tttttggaat cacaagtcaa tatgagaata aatcagacca aataaagaga gtgtattttc
    19081 ctattgcaga atgggaaaaa gtaggtctaa agaagcaatc atggattgac gtagggtctt
    19141 taaaggctat tcccaaaagc cgagagaaca tatcatttaa aaaggttggt atgctatcag
    19201 ctattgatac gaaaaagcta aataggttta tcgagagatt acaaagaaaa gtctaacaag
    19261 gcttttcttt ttttgtttat atgtatatta agatatacgt ttatataaac gtatttatgt
    19321 atattaaaat acacgtaaat atatacgtga ttataaaaat taagaaaata ttaagtattg
    19381 ttaaatagtg ataattagta atatacgaat tataagatac attttatatt taatgtatgt
    19441 aaataaataa aatgactgac ggatattgaa tagtaataaa taacaataat tcagacggat
    19501 acagtgagtg aagtagcttg ctacgaacga acaggcagaa tgaaacggta gtttcattag
    19561 agcaagtcgc ttgcgacgta gcgttaccac tgatgtaact ggcaagttac atagagagag
    19621 tttgcttgca aacgaacgtt acccacttga atagaaaaca aaaataacat attagtgaat
    19681 tttgaatagg agccacaact caaccaaccg cttgcggtgg gtgagtccca cggaggaaaa
    19741 gacgaacgaa cggtcgcgta gc
//
LOCUS       DANKBF010000056        19694 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000056 DANKBF010000000
VERSION     DANKBF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..210)
                     /locus_tag="PWY89_002100"
     CDS             complement(<1..210)
                     /locus_tag="PWY89_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067116.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="PWY89_002101"
     CDS             complement(216..446)
                     /locus_tag="PWY89_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067117.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="PWY89_002102"
     CDS             1063..1725
                     /locus_tag="PWY89_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067118.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="PWY89_002103"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="PWY89_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2067119.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="PWY89_002104"
     CDS             5145..5459
                     /locus_tag="PWY89_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2067120.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="PWY89_002105"
     CDS             5472..5846
                     /locus_tag="PWY89_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2067121.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="PWY89_002106"
     CDS             5856..6200
                     /locus_tag="PWY89_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2067122.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="PWY89_002107"
     CDS             6271..7623
                     /locus_tag="PWY89_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2067123.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="PWY89_002108"
     CDS             7683..8825
                     /locus_tag="PWY89_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL2067124.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="PWY89_002109"
     CDS             8850..9104
                     /locus_tag="PWY89_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067125.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="PWY89_002110"
     CDS             9360..10544
                     /locus_tag="PWY89_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2067126.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="PWY89_002111"
     CDS             10541..10678
                     /locus_tag="PWY89_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2067127.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="PWY89_002112"
     CDS             10683..10904
                     /locus_tag="PWY89_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067128.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="PWY89_002113"
     CDS             10917..11417
                     /locus_tag="PWY89_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2067129.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="PWY89_002114"
     CDS             11514..13361
                     /locus_tag="PWY89_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL2067130.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="PWY89_002115"
     CDS             13364..14260
                     /locus_tag="PWY89_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2067131.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="PWY89_002116"
     CDS             14310..14699
                     /locus_tag="PWY89_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2067132.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16188
                     /locus_tag="PWY89_002117"
                     /pseudo
     CDS             14686..16188
                     /locus_tag="PWY89_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="PWY89_002118"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="PWY89_002118"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2067133.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18893..>19694
                     /locus_tag="PWY89_002119"
     CDS             18893..>19694
                     /locus_tag="PWY89_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2067134.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DANKBF010000057        18919 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000057 DANKBF010000000
VERSION     DANKBF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(46..1365)
                     /locus_tag="PWY89_002120"
     CDS             complement(46..1365)
                     /locus_tag="PWY89_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2067135.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1362..2015)
                     /locus_tag="PWY89_002121"
     CDS             complement(1362..2015)
                     /locus_tag="PWY89_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2067136.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2303..2823
                     /locus_tag="PWY89_002122"
                     /pseudo
     CDS             2303..2823
                     /locus_tag="PWY89_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2725..3441)
                     /locus_tag="PWY89_002123"
     CDS             complement(2725..3441)
                     /locus_tag="PWY89_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL2067137.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3565..3750)
                     /locus_tag="PWY89_002124"
     CDS             complement(3565..3750)
                     /locus_tag="PWY89_002124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067138.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3977..5250
                     /locus_tag="PWY89_002125"
                     /pseudo
     CDS             3977..5250
                     /locus_tag="PWY89_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5357..6388)
                     /locus_tag="PWY89_002126"
     CDS             complement(5357..6388)
                     /locus_tag="PWY89_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2067139.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6395..7225)
                     /locus_tag="PWY89_002127"
     CDS             complement(6395..7225)
                     /locus_tag="PWY89_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2067140.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7222..8037)
                     /locus_tag="PWY89_002128"
     CDS             complement(7222..8037)
                     /locus_tag="PWY89_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2067141.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8034..8858)
                     /locus_tag="PWY89_002129"
     CDS             complement(8034..8858)
                     /locus_tag="PWY89_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDL2067142.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8848..9948)
                     /locus_tag="PWY89_002130"
     CDS             complement(8848..9948)
                     /locus_tag="PWY89_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2067143.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10126..10911
                     /locus_tag="PWY89_002131"
     CDS             10126..10911
                     /locus_tag="PWY89_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDL2067144.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10944..11327
                     /locus_tag="PWY89_002132"
     CDS             10944..11327
                     /locus_tag="PWY89_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2067145.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11403..11534
                     /locus_tag="PWY89_002133"
     CDS             11403..11534
                     /locus_tag="PWY89_002133"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2067146.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11503..11739
                     /locus_tag="PWY89_002134"
     CDS             11503..11739
                     /locus_tag="PWY89_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2067147.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11817..12788)
                     /locus_tag="PWY89_002135"
     CDS             complement(11817..12788)
                     /locus_tag="PWY89_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL2067148.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13440..15062
                     /locus_tag="PWY89_002136"
     CDS             13440..15062
                     /locus_tag="PWY89_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HDL2067149.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            complement(15348..16724)
                     /locus_tag="PWY89_002137"
     CDS             complement(15348..16724)
                     /locus_tag="PWY89_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2067150.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(16724..17380)
                     /locus_tag="PWY89_002138"
     CDS             complement(16724..17380)
                     /locus_tag="PWY89_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2067151.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(17390..18667)
                     /locus_tag="PWY89_002139"
     CDS             complement(17390..18667)
                     /locus_tag="PWY89_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2067152.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(18686..18847)
                     /locus_tag="PWY89_002140"
     CDS             complement(18686..18847)
                     /locus_tag="PWY89_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL2067153.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT         5835 a         3899 c         3060 g         6125 t
ORIGIN      
        1 aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag aaaatatttt
       61 tattttaaag ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt
      121 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
      181 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
      241 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
      301 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
      361 ctcgtctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
      421 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt
      481 ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc
      541 aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt
      601 ttccagtatt attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa
      661 aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc
      721 aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc
      781 atcattagag ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac
      841 aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc
      901 agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc
      961 atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag
     1021 ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat
     1081 attatctttg atttgtattt cattcttatt atttttttct tttatgaata cttttatttc
     1141 actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat
     1201 attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt
     1261 tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga
     1321 aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta
     1381 tatcctacat ttcttatagt agttatacag tctaattgta atttttttct aagttctttt
     1441 atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca
     1501 ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct
     1561 agttcttttg catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa
     1621 ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa
     1681 gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact
     1741 aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt
     1801 ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc
     1861 tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca
     1921 tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt
     1981 atcatattat catcttcaac agttaatatt ttcatatata cctccactta agcctataca
     2041 ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt
     2101 gccaatactt tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaaaa
     2161 aaggcttcta taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt
     2221 ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc
     2281 accctaccaa aggagtcgct ctatggataa tcatactaga aaattactcg gattaacaga
     2341 taaacattta ttttttgatg aagaatggct gattgaaaaa gaatataaag gcgttcaagc
     2401 gcagtttatt aaagggagat tggatgactc accttcccat tgtgaacact gtggatcgat
     2461 tcgtattatc cgcaacggtt cctatacgac acgtactcag atgttgaaag tcaaagaaaa
     2521 gttgactatt ttagaattaa aacgtacccg ttttctatgc tatgattgtg ggcagacctt
     2581 ttctgcgaaa acggacttag ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc
     2641 gattctcata gaactgtacg aaaatcaatc acgtaatctg aaaatcagaa gaagtaccgt
     2701 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
     2761 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
     2821 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
     2881 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
     2941 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
     3001 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
     3061 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
     3121 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
     3181 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
     3241 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
     3301 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
     3361 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     3421 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     3481 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     3541 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     3601 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     3661 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     3721 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     3781 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     3841 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     3901 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     3961 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     4021 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     4081 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     4141 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     4201 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     4261 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     4321 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     4381 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     4441 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     4501 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     4561 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     4621 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     4681 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     4741 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     4801 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     4861 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     4921 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     4981 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     5041 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     5101 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     5161 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     5221 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     5281 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     5341 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     5401 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     5461 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     5521 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     5581 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     5641 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     5701 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     5761 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     5821 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     5881 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     5941 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     6001 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     6061 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     6121 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     6181 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     6241 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     6301 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     6361 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     6421 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     6481 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     6541 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     6601 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     6661 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     6721 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     6781 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     6841 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     6901 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     6961 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     7021 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     7081 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     7141 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     7201 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     7261 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     7321 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     7381 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     7441 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     7501 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     7561 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     7621 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     7681 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     7741 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     7801 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     7861 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     7921 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     7981 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     8041 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     8101 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     8161 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     8221 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     8281 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     8341 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     8401 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     8461 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     8521 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     8581 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     8641 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     8701 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     8761 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     8821 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     8881 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     8941 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     9001 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     9061 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     9121 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     9181 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     9241 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     9301 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     9361 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     9421 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     9481 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     9541 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     9601 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     9661 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     9721 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     9781 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     9841 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     9901 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     9961 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
    10021 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
    10081 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
    10141 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
    10201 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
    10261 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
    10321 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
    10381 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
    10441 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
    10501 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
    10561 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
    10621 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
    10681 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
    10741 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
    10801 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
    10861 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
    10921 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
    10981 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
    11041 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
    11101 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
    11161 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
    11221 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
    11281 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
    11341 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
    11401 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
    11461 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
    11521 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
    11581 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
    11641 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
    11701 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
    11761 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
    11821 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
    11881 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
    11941 aaaagctcta ttcttgagct ggctgcagct agtgagtgtc tttcatttat ccataagata
    12001 aagcttggat tcaataaata aataaatgtt ttggttaaag ctgtgaccag caaatgagga
    12061 accgtgcgag ccagttgatc tgccaatgca aacatttctt taggtgaagg cgctggcgtg
    12121 tctgccaacg gatactccag tcccaatgct tggtaaccgt gcataccata gcagtggtac
    12181 ggtagtaatt ctaacggtac gaaccgatca agatctgcca caaaacaagc aatcgtcagc
    12241 agctcggtca ggttcatatt gatacctgga accacaggaa ctcgaattcg cacttcgcag
    12301 ttaggatact caattaaaaa gcgacgaata ttcttcaaaa tttgttggtt atctaagccg
    12361 gtccattgtt tgtggagctt gctggtaaac atttttacat ccacaaacaa tgtatccaca
    12421 tagggagcta ccttttggat ttcattataa gcaccacaaa agctggtttc cagtacggta
    12481 ttgattccaa tgtatttcga tttttgcaaa atttctttag caaaatccgc ctgaaccagt
    12541 gcctcgcctc cgctaatggt cactccgcca tccgagtgaa agtaaaaaac agcatccttc
    12601 tcaatctcat ccattacctc ttcagcggtc atggtctggc caaatccttt ttcaatctgc
    12661 ttgctttgtg attcgggaat cgaacaccag gcacagcgta atgggcagcc ctttaaataa
    12721 acaacggtcc gtaaaccagc accatcatgt agagaaccct gttctattct taatactgtt
    12781 ccttccatat agatttttga attacacact agtttaaact aaaacacaca aggaaaaact
    12841 taccatttaa tcataaaatt agctcatata aatatactag gatgtaaaat agactggtca
    12901 aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat tttaatattc
    12961 taaatcattt tttattggtt tattccctta ttcgatgtag actcaaatca ctatatcact
    13021 agatatcaaa cggcgtcata cggaatcaaa aacatcaaat ttgacctgtt aaatggtcat
    13081 tgaataacat tcaaaatcaa taaaacggag tcagacacgg agtcaaaaac gctgagagta
    13141 aaaaatatat acagccttgt aggtaaagta ccacagggct gtatattttt tatttcagat
    13201 taccttaaat gattccattt actttttatc tcattttttc ctactagtta gtatcaaata
    13261 ctctcctatt ttctcaataa actatttttg gatacatggt atctgaaaca gctaaaacct
    13321 tgtcattaca gcaaaaaatc acgttatcca gactgtagac aaagtccatt ttatggatga
    13381 tgtctacagt cttttctttt ataatagatt tatgaaaacg aatgcaagga gtgatagcta
    13441 tgatgtctaa aaacaatacg aatggtagaa atcaatttgc gatgcttacg attgatgatc
    13501 tagttcctca agatcatctc gttcgaaaga ttgatgccgc gcttgatttt gaatttattt
    13561 atccaatcgt cgaagccact tactccgatt taggtcgccc aagtatcgat ccagtgattc
    13621 tgatcaaatt ggtgtttatt cagtatttat tcggtatccg ttccatgcga caaaccatta
    13681 aggaagtcga taccaatgtc gcttaccgct ggtttttagg ctactccttc gaggagaaaa
    13741 tccccccttt ctcaactttt ggaaagaatt atgttcgccg atttagagaa acgacggtgt
    13801 ttgaggatat ttttgcctat attttagaac aagcagttaa agcaggtttt gtaacagagg
    13861 ataatttgta tctggattct acccacatca aagctaacgc caataaacat aagtttacga
    13921 aagaaatgac ccatgatgaa gcgaaagcct atcaggatga attagaagat gagatcaatc
    13981 gtcaacgaat agaggctgga aaacgccctt ttactttgga cttagaaaag gaagtgaagc
    14041 tgaaagaaag aaaaataagt aaagctgatc cggaaagcgg ctactacgtc aaaggcgaac
    14101 gagaaaaaca atttgcttat tctgtccata cttcatgcga cgacaatgga tttattctta
    14161 gcacaatcat cactcctaga aatattcatg acagtcaagt cgcctttcag ctcgtcaaac
    14221 aatcgaagag actttttcct gaaatcaatt gtgttgtcgc tgatgctggg tataagacac
    14281 ctaagtttgt tcactttttg actcatttga accttcgacc ttgtttgcct tattcaaggc
    14341 ccaaagggaa aaaggagcta ttatcaaaaa atgagtttct ctatgacgag tattttgatt
    14401 gttatatttg tccgcaagat caaatgctcg ctttttcaac ggttactcga gaagggtacc
    14461 gagaatacaa atccaatcca aaagaatgtg tgaactgtcc tctgctaaac caatgtacca
    14521 taagtaaaaa tcatcaacga gtcatcacga gacatgtttg gggagatctg atggatgaag
    14581 tggaacatct tcgcctaaca gatctgaata aatcaattta caaaaaacgg aaacaaacga
    14641 ttgaacgaat atttgcggat gcaaaagaaa agcatgggat gcgttggacc aagtatcgag
    14701 gcttggaaaa agtcgcgacg catacgatgc tagtgtttgc tgcaatgaat ttaaagaaat
    14761 tagccacctg gctatggaaa ggcaaggagc ctttattttt ttgttcaaaa attcgaaacg
    14821 aagtagataa aaagctgttc caagctcgag ttacgagttt ggaacagctt ttgtctacag
    14881 tctgcgatgt cggagacatc tcgttttcta ttaggaatca actcaggttc aaaagaagcc
    14941 tcccggtctc tgggaacatc aatgtttact ttaccaaagc tcgttttaag cgtcttattg
    15001 ccataaccgt ttcgtcgatt gtcatgtttc tttggctctt tagaatgggc gtcataccct
    15061 aaatggttat tcaattctcc ttgaagcatt ttttcaaata agggaccaaa cacatctttt
    15121 aaaacatctt gcatatcctc tacagattcg ggttgataag tattcaaaat ggattcagct
    15181 aacttctcag catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
    15241 attttagaaa aataaaaacc gtgaagcaac cacccgccta ggattgatgg ttacttacac
    15301 ggtttatatt acactctcat ttttggtgct aaaaagcacc gaaaatttta attattgtca
    15361 attaataatt cttttggatt ccttcttaat atattgaatg aagaaataat caatgataca
    15421 agaagtacta aactcataaa tataactaca taaatcatga atttaggtaa tacattgata
    15481 tctaaacttg ataatgtctt gttaaatcct tcagcttctg ccccaccacc taattggcta
    15541 gatgcagatt gtttagctat ttgtttagct atatcaccag taaccttatt caatatatta
    15601 ttcccaagct tgtcagctgt atattgagct aagaaatagg aaccaacgaa tgcggggatt
    15661 gatataaata ccatctcaat taggaattga ccaaaaattt ctaattttga tatacctaat
    15721 gatagtaata ctgctatttc tttctttcta gcattcatcc ataagaataa taataatgaa
    15781 actacaactc ctgcaaatat taatgagcca aaaaataatt tatttgaaat tgagtaaata
    15841 cctgatatag attgttgtaa tgcagggtaa tttgatgaac ttttaatcaa attatattct
    15901 ctccaattta tatctaattt cccaagatct tttattacac tatcaagatt cttatcacct
    15961 tttacaaaga atgttgcatc ttggtataca gctgtatctt cggtattacc ataaacttta
    16021 gcagctgtat gaatatcagt gattaatgta ttttcgtata gttcttgtgc tgcacttact
    16081 acaccgctat tatgcccatc gaatagacct ttaatttcta cttctactgt ttcatctgca
    16141 cctttctcat tatcagcatc aaataaatta gattttattt ttatcttatc tccaacttta
    16201 aggttatttt tttcagctaa atctttatgc attaagattt tatttttatc ttcattttct
    16261 aaatgctttc cttctactaa tttatatgct tctgatacaa attttgtttc ttttgttgag
    16321 tcgttaaccc cagttaacat aactgttctt ttaaaattct ttgctctttc aggtgattga
    16381 ttcgcaagag tctcttgagt ttcaataata tcataatcaa ctaaatctgc aacactgttt
    16441 attcttttta cataggagtc tatactatct gtttgagata tctttttaat atcttcacct
    16501 tttacatttc ccccacctct aggtgttcct ggatttactt gtctatttat ctccatagaa
    16561 aaactatttg ttatattagc aaatgtttct tttgaagctc tgtccgtagc atctttaata
    16621 gataaactaa tgagacttaa agttgacata gataaaataa ctaatatgat aattaatgat
    16681 tttaaacttt ttctagttac ataagcaaat gcatttttta tcattattca acctccaaat
    16741 tcattttatt tacttttttc aactttttac cacttagttc taaaatgata tccgcagaat
    16801 ctgctacttc cttactatgt gttacaacta ttacacattt atttctatct ttagctaatg
    16861 tctttaatat attaattatt tctccagcag taacactgtc tagattacca gtaggctcat
    16921 cagctagtat tattggagca tctgatacca atgctctagc aatagctact ctttgttgct
    16981 gtccaccaga taatttcata acatttcttt ttatttgttt tttatctaaa cctagttcaa
    17041 acaagatact ttcatctgct gatttattta ctaatctaat attttcaatt ggtgataaat
    17101 aatctattaa attatagttt tgaaatacta aagatatatt attttttcta tgattactat
    17161 atcctttatt ttgtatatct tcattcttaa acaatatttt tcctgtttga ggtttatcaa
    17221 gtccagcaag taaggaaaga agtgtagatt ttcctgttcc tgactttcct actatcgcat
    17281 aaaacttccc aagttcaaat ttttgattta ctcctgacaa aactttttct ttagaatttg
    17341 cataactata tgctacattc tttatttcta atatatccat tattttctcc taacttattt
    17401 ttgataagat ttctttaggc ttcttgatta acattaatga agaagcaaat acaactgata
    17461 aaacaataat acttattaat attaaataac tttgtccaag tgttgttata tttgacataa
    17521 aactactatt atttattaaa cttccaccgg aaatcattga atcctctgag ttaatgaatc
    17581 catctacaat tacttttagt aatacatttc ctaaaaataa ggaagatatt atacttggta
    17641 tcgatatgaa tattaactcg aatataaatt gcattataat ttgtatctta gatgttccaa
    17701 tagataagaa tatacctatt tcataaattc tttcccttaa ccatagaatc aagattaatg
    17761 aaagaacaac catcccacct aacataatag aataagtcat tacttttatt atatatttta
    17821 ttccactcac tgactctaaa gactcttcaa acgcattaga atctttttca acaaaatact
    17881 ttgactcatc aattttaagc tctttcaatt tgtttaaggc taagtctgta gattctgcac
    17941 taccagaata cattaaaatt ttatttgcaa tttcattatt ttctgaatta tttaatatct
    18001 cttggcttgt tgaataatct acaaacacca tattttcact aaaatcagaa gataatcctg
    18061 tatatgtttc ctgtttttta ccagaaaaga tccctataat tttaaattta tgacttttta
    18121 tttttccact tttttcaata tctagtaatt caagatcaac ttcatcacct aattttagat
    18181 tgttttgttt agcaaattct tcatgaacaa taattgaatt cttatcattt tcttctatat
    18241 ttttaccttc tttaattgta aatactccac tactaaataa aatatttctt ttagtattat
    18301 ttgtagcttc gagtgaaaca acattcttaa attcgtcaga taaatcttct ctatttattc
    18361 tttgttctcc gctaactact ttagcatctt ttagttttgc taatccatca tattgaatta
    18421 ttttttcttc aatttctttt aatttttcaa tatctttaaa ttgattaaca ttaaaatatt
    18481 taccatcttt ttttgttatt gatattgaag aattagaact ttcatataaa gccttttcta
    18541 tttcattgct tgatttcatt attgttaaac aagaatacaa gcaagaaaga actatagtta
    18601 atatgataaa aattattaaa gttctatttt tctttcttgt aatatatgct atagcatttt
    18661 ttatcacttt cctaactact cctctctatt ttcttactcc ataactaatt gcatccactg
    18721 gacaagcatt tttacatcgt ccacatctga tacactccag atgattgcaa ttctcaactg
    18781 aatctttatt catttggcaa acttttttac attttccaca attaatacac ttatccttat
    18841 cctgaatcgg ctacactaga ctagacagaa aaaataaggt gtgtagaata ataaaaaaca
    18901 cactggagga tttttcatg
//
LOCUS       DANKBF010000058        18301 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000058 DANKBF010000000
VERSION     DANKBF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            502..1059
                     /locus_tag="PWY89_002141"
                     /pseudo
     CDS             502..1059
                     /locus_tag="PWY89_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1117..3156
                     /locus_tag="PWY89_002142"
     CDS             1117..3156
                     /locus_tag="PWY89_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL2067154.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3265..4416)
                     /locus_tag="PWY89_002143"
     CDS             complement(3265..4416)
                     /locus_tag="PWY89_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2067155.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4570..5100)
                     /locus_tag="PWY89_002144"
     CDS             complement(4570..5100)
                     /locus_tag="PWY89_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2067156.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5199..5549)
                     /locus_tag="PWY89_002145"
     CDS             complement(5199..5549)
                     /locus_tag="PWY89_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL2067157.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5706..7235)
                     /gene="ahpF"
                     /locus_tag="PWY89_002146"
     CDS             complement(5706..7235)
                     /gene="ahpF"
                     /locus_tag="PWY89_002146"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL2067158.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7245..7808)
                     /gene="ahpC"
                     /locus_tag="PWY89_002147"
     CDS             complement(7245..7808)
                     /gene="ahpC"
                     /locus_tag="PWY89_002147"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL2067159.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7952..8614)
                     /locus_tag="PWY89_002148"
     CDS             complement(7952..8614)
                     /locus_tag="PWY89_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2067160.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8798..10444
                     /locus_tag="PWY89_002149"
     CDS             8798..10444
                     /locus_tag="PWY89_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2067161.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10435..11517
                     /gene="uxuA"
                     /locus_tag="PWY89_002150"
     CDS             10435..11517
                     /gene="uxuA"
                     /locus_tag="PWY89_002150"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2067162.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11625..13178)
                     /locus_tag="PWY89_002151"
                     /pseudo
     CDS             complement(11625..13178)
                     /locus_tag="PWY89_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13326..14467
                     /locus_tag="PWY89_002152"
                     /pseudo
     CDS             13326..14467
                     /locus_tag="PWY89_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14593..15672)
                     /locus_tag="PWY89_002153"
     CDS             complement(14593..15672)
                     /locus_tag="PWY89_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2067163.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15781..16221)
                     /locus_tag="PWY89_002154"
     CDS             complement(15781..16221)
                     /locus_tag="PWY89_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2067164.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16278..16688)
                     /locus_tag="PWY89_002155"
     CDS             complement(16278..16688)
                     /locus_tag="PWY89_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2067165.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16869..17303)
                     /locus_tag="PWY89_002156"
     CDS             complement(16869..17303)
                     /locus_tag="PWY89_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2067166.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17403..18251)
                     /locus_tag="PWY89_002157"
     CDS             complement(17403..18251)
                     /locus_tag="PWY89_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2067167.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         6285 a         3386 c         3295 g         5335 t
ORIGIN      
        1 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca atacaaccag
      541 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
      601 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
      661 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
      721 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
      781 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
      841 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
      901 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
      961 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
     1021 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
     1081 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
     1141 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     1201 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     1261 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     1321 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga taaataccag
     1381 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     1441 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     1501 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     1561 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     1621 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     1681 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     1741 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     1801 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     1861 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     1921 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     1981 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     2041 aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga cggtgtagag
     2101 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     2161 gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc aactgttgcg
     2221 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     2281 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     2341 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aatgattaca
     2401 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     2461 cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat
     2521 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     2581 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     2641 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     2701 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     2761 atccagcaag cagcaatgta ttctgttttt atcaacaatg gcacacttgt ctatcctaaa
     2821 ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     2881 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     2941 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     3001 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     3061 atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa acgagcatcg
     3121 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     3181 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     3241 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     3301 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     3361 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     3421 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     3481 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     3541 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     3601 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     3661 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     3721 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     3781 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     3841 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     3901 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     3961 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     4021 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     4081 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     4141 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     4201 actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa agtcatggac
     4261 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     4321 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     4381 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     4441 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     4501 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     4561 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     4621 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     4681 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     4741 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     4801 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     4861 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     4921 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     4981 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     5041 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     5101 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     5161 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     5221 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     5281 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     5341 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     5401 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     5461 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     5521 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     5581 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaaag
     5641 gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt tacaatcttc
     5701 atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc tcctgaaccc
     5761 attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc gaaaacgccg
     5821 gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt catttcgatc
     5881 gttctatcta gccaatccgt attagggagt aagccgacta gaatgaatac tcctgaaaca
     5941 gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc tacctgattc
     6001 gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc aagagagact
     6061 agtttatcct gtaagatttg atctgctttt aattctggta agaattctag aacatataca
     6121 tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc accaccgata
     6181 acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata agcaattcct
     6241 ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc acctgtcgcg
     6301 atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt aataaaatgt
     6361 tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg gtaactacgc
     6421 acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt accaatcata
     6481 ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac aagtcctgta
     6541 cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc accgccaatc
     6601 actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg gccagtgatt
     6661 ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc atttaaaaac
     6721 acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat cccaccttca
     6781 atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc ctgtacaacg
     6841 tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt ttgtgttatt
     6901 tttttgattt taagactcaa agattcactg attttcggtg cccgtccact aatttgtaac
     6961 atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa aacgattcca
     7021 ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc tcttttttcc
     7081 caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag tttttgacta
     7141 ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag tgctaaatat
     7201 tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat ctttccaaca
     7261 agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg acaaacttca
     7321 cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc actagcgtca
     7381 cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg atcaataata
     7441 aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat acgagaaatc
     7501 tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc tgatgtatca
     7561 tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc aacacctaaa
     7621 gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca aacaaaagta
     7681 aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt ttcactgact
     7741 tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt tttcccaatc
     7801 aatgacatag taaaacctcc aataataatt atttatttat aattattgta atttagttgg
     7861 agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa cgaaggttaa
     7921 ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg ggataacgtt
     7981 gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt tgaaatcgag
     8041 cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt tccatgatcg
     8101 tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct aaatgggtat
     8161 tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa caagcttcat
     8221 gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact gctttttctt
     8281 gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg gaacaacatt
     8341 caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct gcagaaacaa
     8401 gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt tcttgctgac
     8461 gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt tcctccagtg
     8521 ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt ttacgaatcg
     8581 cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa tcatttctat
     8641 gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt aactagtaaa
     8701 tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa aattcacttg
     8761 aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa acaactggag
     8821 gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg accgtgcatc
     8881 attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg gaaatcttta
     8941 tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat tgtccactgg
     9001 tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc aagattttga
     9061 tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac gtatattagc
     9121 tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg ataagatgat
     9181 tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg aaaaaggtta
     9241 tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata taaaaaatgg
     9301 tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata aacgtttttt
     9361 atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa acggtagtca
     9421 attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt atgtacaaaa
     9481 tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga cgatgattga
     9541 tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag agggcttttc
     9601 aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg tcaatacaga
     9661 ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga aattcgaaaa
     9721 agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga tgaaggtgac
     9781 gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac tggggtatag
     9841 gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg aaatcgggta
     9901 caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga tctttctgga
     9961 agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc agcgaatcgc
    10021 cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa aatatatgga
    10081 aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg ctgcttggct
    10141 tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc cagacccact
    10201 attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag atacatcaca
    10261 tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg tagatcttta
    10321 ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag caggaccaaa
    10381 tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt tatcatggaa
    10441 atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga aatcagacag
    10501 attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg agaggtatgg
    10561 cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt aacactaaaa
    10621 gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc ccgtgagcag
    10681 tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat agaagtcatc
    10741 tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta ccgcttagaa
    10801 gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc aagagaaatc
    10861 gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga acctgagcgc
    10921 ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga aaaacttcga
    10981 gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt aggggtcaaa
    11041 atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg aatcgtaaag
    11101 aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa cggaatcact
    11161 ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat tttagcagaa
    11221 ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt tttagctaat
    11281 cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat gtaccgcata
    11341 ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca tggaagaatg
    11401 atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt aggtgctgtt
    11461 tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga taattagtga
    11521 aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga tacataaatc
    11581 cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga agtaacttga
    11641 ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat atattctgag
    11701 atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa aatatagatt
    11761 tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat gttgcagttc
    11821 tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc tatcttaacg
    11881 atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac aattcgcggc
    11941 tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg cacattttcc
    12001 ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat ataaaattgt
    12061 tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat gaaagtagga
    12121 aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt tcgtgcgaat
    12181 gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac agtacaaagg
    12241 gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt taaaagtttg
    12301 tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg agctaaaata
    12361 ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat atctacagag
    12421 agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac gtctttcaca
    12481 gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt atctatcata
    12541 ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat aaaagccact
    12601 acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt ccaataaata
    12661 tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct gccattatta
    12721 gaaaaggaga tagacaaacc gtactatgct aaaaatgcat taatcttttt cattttcgta
    12781 tacaaataag aggcactata gtttgtttgc tgactgaaac tgataatcga atttgtctga
    12841 cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta tcttccaaat
    12901 agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa aaaaggtttt
    12961 tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc taattctttg
    13021 atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata ttgtgtagta
    13081 ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt gctttttttc
    13141 ttcaagataa acgttttaat cgcttcttga gtttgcatga aagaggctcc tttttactta
    13201 aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa aaaacagtaa
    13261 acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca aggagtgaga
    13321 gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc ttatcgctta
    13381 ctataaatca gacagtccac acagaggaga cagaaacagc caaatggaca gaaaccaagt
    13441 gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat tcaggagtcg
    13501 caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat tcgggagact
    13561 tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccact ttgtcgattg
    13621 gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc ttcaccgtta
    13681 cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca gatggactac
    13741 cgaacgatca tcatttaacg ccgtatagtc agcggcagac aattgagcaa gattcagcca
    13801 tcgactcagg aggagatctt gttgaagaac caacagataa accagaaaat gaaaataagc
    13861 cagaagtacc gcctacagaa aatccagacg gagaacagaa accggaaata gaacctggag
    13921 aagaacccga tacagaaact caaccagagc cagacaataa atcaaaacct gaaataaccc
    13981 ctggagaaaa accagatgta actccagaac cagatacgga ttctggaaac caaacagtac
    14041 cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca gaggtagatc
    14101 cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac caaggaatac
    14161 cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt aaaaaatcaa
    14221 atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga atatttgata
    14281 gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct gtcctgaaac
    14341 gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg
    14401 aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc
    14461 tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa
    14521 aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc
    14581 ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac
    14641 tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc
    14701 aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt
    14761 gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt
    14821 aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg
    14881 aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc
    14941 taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtatt
    15001 taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc
    15061 ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga
    15121 gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc
    15181 aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt
    15241 ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg
    15301 atctgcatct tttggctgat gctcgtaaaa atcaataacg gactggatca tatcaaatgt
    15361 ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag
    15421 tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg
    15481 aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact
    15541 tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc
    15601 attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa
    15661 cacgttattc attatatgat ggaatatgaa aataagaaag agaaagagca aaaagaaaaa
    15721 ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca tctagcagtt
    15781 tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca gatgttgcag
    15841 tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg gaataccttc
    15901 tgcttgtttt tgcaacgcag caccttgagg agtcagcgta acatttacga ttctttcatc
    15961 acccgtttca cgctgacgat taatataatt tttttctgct aactttttta gtaagggagt
    16021 cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag tctttttctt
    16081 ttcccataaa actaacatca caagatactg ggtataggta agattcagcg gcttgaggag
    16141 ggaagtgtag tgtctaatga tttccttaga ggcggcatat aaaggaaaac atacttgatt
    16201 aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg tatcatagcg
    16261 gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat atttccagct
    16321 gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg cgtttcttcc
    16381 agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct cgtttcactt
    16441 tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt tttttgctgt
    16501 ttgatcaatt ctaatgcact gttaaaacat gcactgaaac cagctgcaaa caattgttca
    16561 gggttggttg cattttcgac gcgtttacca ggagctgcaa tattcaattc gaatgtacga
    16621 tcgggattat gaacttctcc agagcgtccg cctgtgttga tcattttcgt agaatacatt
    16681 tttttcataa aattaccacc tttaattata ttttatacaa ttgaatttta cacaatataa
    16741 ttgtggaaag caaagaaatg gattggcata agataaataa gagaaagcac atgaaacaga
    16801 cgcgctttct cgaatagcaa acatatacaa aaaaatatca gagacaaaaa ctaatctcat
    16861 cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaact tacttcacga
    16921 tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca
    16981 ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg taatgcttta
    17041 ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca
    17101 gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtgcca
    17161 tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt
    17221 atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa
    17281 tctatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag
    17341 attcttttat tataccagtt aaagaaaacg cattcaaaaa aatagcggat ttacttacca
    17401 actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca
    17461 atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga
    17521 tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct
    17581 agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa
    17641 tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg
    17701 acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaaca
    17761 aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag
    17821 tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt
    17881 ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac
    17941 cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa aaacaagctt
    18001 ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata
    18061 acgatattca acactaaaat aactatagct cggtcaaatc ctaaaacagc ttccagtaat
    18121 attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata
    18181 gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc
    18241 gtttctttca tttatccact tccttaattc attaataata ctcttatctt attataactt
    18301 a
//
LOCUS       DANKBF010000059        18043 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000059 DANKBF010000000
VERSION     DANKBF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(38..1189)
                     /locus_tag="PWY89_002158"
     CDS             complement(38..1189)
                     /locus_tag="PWY89_002158"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2067168.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="PWY89_002159"
     CDS             complement(1202..1639)
                     /locus_tag="PWY89_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2067169.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="PWY89_002160"
     CDS             complement(1654..3420)
                     /locus_tag="PWY89_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2067170.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="PWY89_002161"
     CDS             3684..4361
                     /locus_tag="PWY89_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2067171.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="PWY89_002162"
     CDS             4364..5491
                     /locus_tag="PWY89_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2067172.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="PWY89_002163"
     CDS             complement(5691..6131)
                     /locus_tag="PWY89_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2067173.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="PWY89_002164"
     CDS             complement(6142..8175)
                     /locus_tag="PWY89_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2067174.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="PWY89_002165"
     CDS             complement(8177..9880)
                     /locus_tag="PWY89_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2067175.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="PWY89_002166"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="PWY89_002166"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL2067176.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="PWY89_002167"
     CDS             complement(10963..11988)
                     /locus_tag="PWY89_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2067177.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12197..13066)
                     /locus_tag="PWY89_002168"
     CDS             complement(12197..13066)
                     /locus_tag="PWY89_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL2067178.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="PWY89_002169"
     CDS             complement(13525..15417)
                     /locus_tag="PWY89_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2067179.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="PWY89_002170"
     CDS             complement(15414..17141)
                     /locus_tag="PWY89_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2067180.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="PWY89_002171"
     CDS             complement(17157..17807)
                     /locus_tag="PWY89_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2067181.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
BASE COUNT         5076 a         3747 c         3166 g         6054 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgcc ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgt
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aat
//
LOCUS       DANKBF010000060        16558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000060 DANKBF010000000
VERSION     DANKBF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            14..1141
                     /locus_tag="PWY89_002172"
     CDS             14..1141
                     /locus_tag="PWY89_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2067182.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="PWY89_002173"
     CDS             complement(1359..2501)
                     /locus_tag="PWY89_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2067183.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="PWY89_002174"
     CDS             complement(2506..2691)
                     /locus_tag="PWY89_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2067184.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="PWY89_002175"
     CDS             complement(3045..3290)
                     /locus_tag="PWY89_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2067185.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="PWY89_002176"
     CDS             complement(3284..3685)
                     /locus_tag="PWY89_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2067186.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="PWY89_002177"
     CDS             complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="PWY89_002177"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL2067187.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5703..7469)
                     /locus_tag="PWY89_002178"
     CDS             complement(5703..7469)
                     /locus_tag="PWY89_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL2067188.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7482..8276)
                     /locus_tag="PWY89_002179"
     CDS             complement(7482..8276)
                     /locus_tag="PWY89_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2067189.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8286..9662)
                     /locus_tag="PWY89_002180"
     CDS             complement(8286..9662)
                     /locus_tag="PWY89_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2067190.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9782..10429)
                     /locus_tag="PWY89_002181"
     CDS             complement(9782..10429)
                     /locus_tag="PWY89_002181"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDL2067191.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="PWY89_002182"
     CDS             complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="PWY89_002182"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2067192.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="PWY89_002183"
     CDS             complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="PWY89_002183"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2067193.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13096..14727)
                     /locus_tag="PWY89_002184"
     CDS             complement(13096..14727)
                     /locus_tag="PWY89_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2067194.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            15031..15702
                     /locus_tag="PWY89_002185"
     CDS             15031..15702
                     /locus_tag="PWY89_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2067195.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         5370 a         3200 c         2222 g         5766 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
     3901 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
     3961 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
     4021 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
     4081 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     4141 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     4201 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     4261 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     4321 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     4381 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     4441 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     4501 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     4561 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     4621 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     4681 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     4741 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     4801 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     4861 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     4921 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     4981 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     5041 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     5101 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     5161 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     5221 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     5281 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     5341 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     5401 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     5461 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     5521 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     5581 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     5641 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     5701 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     5761 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     5821 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     5881 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     5941 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     6001 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     6061 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     6121 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     6181 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     6241 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     6301 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     6361 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     6421 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     6481 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     6541 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     6601 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     6661 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     6721 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     6781 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     6841 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     6901 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     6961 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     7021 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     7081 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     7141 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     7201 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     7261 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     7321 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     7381 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt
     7441 ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt
     7501 ttccaaaaag tatctatata tttttgttca atatctacat aatctcccga taaacattta
     7561 aataaaacat cagttcttaa gttttcccat gattcttttt ctttattgac caaaatagga
     7621 taaaagaaaa ctttattttt atttgcagct tctagatcac caggagaatc accaatcatt
     7681 aatatcttat ccaaatcaca accattttca attacacttc gaataatatc ttcttttttt
     7741 cctttatctt gacaacacag ttcatcaact aatgataata attcatgtct ttcccattct
     7801 tcttgaacag cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga
     7861 gcttttatag tatctctagc acttaaaaag ggtttatcta atccttttgc ttgactaatc
     7921 tgttgattga cttcttccga ccattttaaa gcttttaata aatcctctga ctgagaatat
     7981 tctatttccc tcttcaaaga tggattagac aattcttttg ttagagatac ccagtgcttt
     8041 aatcgattaa tcgatgttac atcatttttt tctaatgttt taactaaacc taaaaatctg
     8101 tttattccac gagtctctga atataaatta atattattcc aatcctgtag gaatttttct
     8161 ctatgatgaa catcaaaaat ctctgcagca attggtccga aaaagagatt gtgtttataa
     8221 gtcatcgtat ccataacaca gccatctgaa tcaatacaaa atatccaatt attcatattt
     8281 ctctcctatt tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt atctttagat
     8341 aatggataaa ggaaaaataa aattaatcct acaatcagaa aacaaattga tggaattaat
     8401 gttgaagcgt tatataactt ttctaaaaca tctcccgttt gtacatttgc taaagagtca
     8461 tagcctataa tagaaagagt ccaacctccg acgcctccag ctagagcttg acctattttt
     8521 ctagcaaaag aatataccgc atatacggtc ccatcttctc tttgatcaga ctttacttct
     8581 tgatcatcaa taacatcagt aatatttgcc caaataacag tattaaacac acccaaaccc
     8641 atatagccaa ccgaactaag aattaagaat acatacatat tactagcttt tatcaaaaat
     8701 aagatcaaat atactattgc tgaaaagaac attcctactg atgccagttc tttttttcca
     8761 tattttttac ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa aataggattg
     8821 ataaaattca ttaaaactat tccctgtgca tcattatagt aatcaggaaa tacataatta
     8881 ttcatactca taattaataa ttgagcaagg agtaagaaaa tagccgcacc aattatagaa
     8941 agtaatgccc tatttttaac taaccctttt aaagatttta acaatgaaac tttgtattga
     9001 gattggtcaa ttcttactcg ttcagtagtt agtttataac atattaaata acaaataagt
     9061 gcacatatcg aaaatattcc cgcaacaatt gtaaaagttg atccaccacg aactatttga
     9121 gcgcctgaat catctcttgt ataaataaat aatggtgtta atacaccaat gaccaaactt
     9181 gctaaagtag ctccaacatt tcggaatgtc gatagcattg ttctttcttc tgtaatagaa
     9241 gtaatagctg atgccattga tccataagga atatttatag atgtgtaaaa aatactcccc
     9301 cataatatat acgtaacata catgtatact accttaacgc tcatagaagc tgaaatcata
     9361 gaagtttgat acattaaaaa acttgctaaa gcgactggac cactcattct taaaatccac
     9421 ggtttaaact taccctgctt agttgctttc gatctatcaa caatacgccc catagttaca
     9481 tcagtaactg catcgataaa tcttgctact aaaaataatg ttccaacggc agctccgctt
     9541 atccccaata cttttgtata aaagatcatt aaaaaagaac tagcaaaaat gaaagtaaaa
     9601 tcgtttccaa aatctccaaa catatatcct attttgtctc ttataccaaa ttttctttcc
     9661 atacaattct ctcccttctg ttgttaagcg ttttcattct gatgaaagta cgtactatac
     9721 tctgaagcgt aaagagtcag tatttatttt ttacatttca gagtattatt tgttttttct
     9781 cctaataatt ctttatctca taaaacttat ctatatattt actagctact cgacttactt
     9841 cttcaaaatc acctttttca acagaagcca ataaattccc acctacacca actgcaacaa
     9901 ctcctacttc aaaccaatct tctaagttct ctatacttac tccgccagta ggcattatat
     9961 ttacgtgtgg taatggagct tgataggctt ttacaatgga tggaccaaaa gcagaacctg
    10021 ggaatagttt aatgatatct acgccagcag taagtgcctc ctttatctca gtaattgtca
    10081 tacaacctgg taaataagga atttgatata aattacaaat ctcagctgtt tgtcgatcaa
    10141 aagatggact aacgatatat tctgccccag ccataattgc gatacgagca gtaaacgcat
    10201 ctaaaaccgt accggctccg attattacat ctttttgatc agcatatttt ttagcaattt
    10261 ctttgattat ttcttcagca ttaggaactg taaaagttag ttcaatccct ttaattccac
    10321 ccgcaataat tgaatttatt gctttaatgg cctcatcctt atttttccca cgtacaaccg
    10381 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    10441 tcctttatgg atacatactt tgtctagctg tactaactta caaagaataa atttaagctt
    10501 ttatttcaag tattattttc tacttccgat ttcaaaaaaa ttaaaataat tatacgcatt
    10561 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcggcc
    10621 tgctttaacc cactcgctta gtaattgaca taatactcga cgaaaatact catggcgagg
    10681 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    10741 ttgtgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    10801 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    10861 tggaagccaa tcagttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    10921 ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    10981 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    11041 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    11101 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    11161 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    11221 accgtgatct gctaatcgac agccaatttg atgaaaatga tccactcgtt ggctcaatgc
    11281 ttgcttcaac ccattaaagt ctttgattgg tatagccgct gtttgactaa gttttaaaat
    11341 atagttcgaa aagcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    11401 taccttgaaa gaattatcct gtttagaaag ctcagtatga taccacaagt catctgtagg
    11461 atcatccgtt gtacagatgg ctttcacatc catcatctcc aacaattctt tcgggcgata
    11521 ggaatccgtt tgaatttttt cgttagcacg tcgccaaatt tctggtgcat tcttctcaga
    11581 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    11641 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcatcacc
    11701 tggaccactg atcaactctt cttctatacc attagctcgc atcaatcgcc atttataatg
    11761 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    11821 tagcaaatat gctgggtcta aatgacaatg atagtcgata attggttggt gttttgcaaa
    11881 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    11941 atttaaacac tcctttcctt ccgttagttt ttagaagaga aaatttttcc tctaaataaa
    12001 ctcccttata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    12061 aaactgcacc tagagctcgg tcatataatc cataaccagg tctaccggtt tctccccaaa
    12121 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    12181 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttcg taaaaatcga
    12241 gtccattgtc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    12301 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    12361 tacataatgt aataccatta gaaggagaat ccactacatt acacaaccaa tctaaatcgt
    12421 cacgattttt aacaatacga ggtaatccga aaatggaata tggtggatca tctgggtgaa
    12481 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    12541 agttctctcg taatttctct tcatcaactt ctttataggc ttctaataat tgcttaattt
    12601 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    12661 ctctaataat ttcctccgga tcagtaggga tattttttga aatataggct aacgtagaag
    12721 acccatcatc taaacgataa tccaaatccg attttaccca atcaaatact ggcataaaat
    12781 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    12841 caatgtattg atctcgtgtt ggtaaaccaa ttttgatatc atcatgaaca ttaacactct
    12901 caataacttt caaggtaagt ccattggctt cgacttcatc ttttaaagat ttaattcgtt
    12961 ctctcggcca aacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    13021 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    13081 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    13141 aactgcttga ggacctacta gcatttcgca aaagattttt tcagttttct ttcctacccc
    13201 aacttgatat aaatctaaac caaagatttc tttattttct aaaatttcct gtaccgcaca
    13261 atgaatcttt gaaggatctt gttcacctaa tttcaatact gataatttat cctgcaactc
    13321 taccaataac ggatcaggac ttggcacaaa attttcccct tgatcatcga ctgccagtaa
    13381 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    13441 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    13501 tgttgcaatt cgttgtggtg tatctggaat atagggatta ggcaaacgtt tattaattac
    13561 ttcatcaata aattgttttg gatcaataat gcctggattt tcaactactg gtaaaccttc
    13621 taaataacct attcctttga ttaaaccaac caaatcatca tcctgcatct ctgctgaaat
    13681 tgattgatat cctagcagac aacctgttac tgccaaagca gtatgaagtg gattcaagct
    13741 tgtcgtaacc ttcatgatgt ctgctttatc aaccgtctct ctatctgtca taatcacacc
    13801 agccttttcc aatttaggcc ggccatttgg aaaactatct tcaataacta aataatgaac
    13861 tacctcagta ttagtgaaag cagcaatatt ggtatgtttt tttgtgtgaa tgatatctaa
    13921 atcctcgagc cccatcatca atagatcttc ctttacattc tctgaaggat ttggcgtaat
    13981 tcgatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    14041 tttttcagaa acatatcctt ctttttgcca agcttctgca atcgtaaaaa tactatcttg
    14101 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gcaactctcc
    14161 agcattaaat ctgctatata acaaggatgt tacaatactc atcgtatgtt gaggattgct
    14221 aggtccagat ttgatatcag ctttgactac cgaaaagaat tgccctttag aatcttttaa
    14281 actgtaacct ttttcagtaa tagtaaacgt tgtaaactgt aatttaggat cttcaaaata
    14341 tttaaccact ctttgatagt ccgctatatt cttcggattg caatataaac tatcagccgt
    14401 tactgccaaa agtctttttt tgagttcacc atctccactc ataacaattt ccaaaatgtc
    14461 attgttatag tcatgataag ctttttccac tacttcttca tcaaatgttt cacacacaac
    14521 tactcctgtt tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    14581 tcgataaaga tttcctccac caaaatgaag ccatactgga tgttgctggc cttcttgctt
    14641 aacttctttg acatcaaaac acggcagtag aatctgcatt tcattgaatt cttcgataga
    14701 attttgccaa tttttaatcg aaaccataat taacctcctc aaactgatat gttagttttg
    14761 atacatttta tattttaaaa cccattgtaa ccgttgtcaa tataaaaagg gaataaactt
    14821 gcttttttct accatatcag aatgattgta ttttttattc agaaaaataa aatactcctt
    14881 ctttttggac tgattttatc gaaacttatt ttttcttttt ttcatgtatt cttttatttt
    14941 tataactagt gtattacttc aaaatttgat attatatggc atgaacagaa ttaaaatatc
    15001 agttaaaata aaagttaagg agcattgtgt atgacaatta aaactagtct ccaaaatcaa
    15061 gcttaccaat ctattcgccg acaaattatc tatgctgact tagaacctgg aaaaaaagta
    15121 tcagaaaaaa aattagaaga aacactttct attggtcgta caccaatacg tgaagcactg
    15181 attcaactcc gacaacaagg tcttgttgat acaattcctc aaagcggaaa ttatatctca
    15241 ttaattgatt tagattcggc caaaaatgct cgatttgtca gagaacattt ggaacgacaa
    15301 attatgttag agtgttgtgc caaaatgaat aaccaaaaaa aacggatttt agaaactata
    15361 atttcagaac aagaaaaagc tgttagtcaa aaaaatgctc gtagtttttt tcataatgat
    15421 aatttattcc ataaagcttg ttttgaaatt gcagagcgag aagaagtatg gcaatggtta
    15481 gaaaataaca atacacactt agaacgattt cgatggctac gagtaaaaac ggaaggatta
    15541 aagtgggaaa aaattatgga acaacattat caactatttg aggcattaac aaaacaagat
    15601 ccagaagaag ctaactttct aacatcgttg catcttcatt taatgcttaa tgagcaaaat
    15661 attgttttag ctactcatcc aaaatatttc aaaaatatat aacttttaac tctttttaaa
    15721 ttaaatggtt gttctgatca gaaaaatatc gcttgttttc ttattatgta atatatttca
    15781 taaactattc tgcataacaa ctattgtttg tactttttac attggataac taattatgac
    15841 tgctgataac ttttgatatt aaactagtat ctccaataca aataaaaaat agatatcttg
    15901 ttcatcaaaa ttagttcgtg gatacttacc ttcatttaca tcattcagcg gtttcccatt
    15961 ttcttttatt ctattatttc attttctaat agacttggct tcatttcgca atactttttt
    16021 tcaataggca tcaaattggt gttgtttaga tatttagttg tagttttgtg acatgataag
    16081 tatgctcctt tttctctttt ccactaatac aacgatcaaa gggtgctttt ttcaaaaaac
    16141 tagaaaaatc aaaataagat tcgtattttg attctatata tcctgtttca actacatctt
    16201 atatttcaca atagaataaa tagccttttg catagctgct taacttataa gaaattgcaa
    16261 tttacttctt caaagtgaaa acgaatatga gtaaaataaa attattcttc ttacagataa
    16321 ttaaaaatag cgcgtaacaa aaatcggcat tcaatcacat aattaggatt gatgccgatt
    16381 acttatattc tacgtatagt tttagtattc ttatttttta tcaccagtaa ccaattgaga
    16441 acagccctaa agcagaatag tcttttatct caaaacgctt aatttcaggc ttaggaatat
    16501 gaataatctt ttgagtatat tctttctctt ttaatcttac tgaagaaata ccactgcc
//
LOCUS       DANKBF010000061        16237 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000061 DANKBF010000000
VERSION     DANKBF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            141..416
                     /gene="rpsP"
                     /locus_tag="PWY89_002186"
     CDS             141..416
                     /gene="rpsP"
                     /locus_tag="PWY89_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2067196.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            428..679
                     /locus_tag="PWY89_002187"
     CDS             428..679
                     /locus_tag="PWY89_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL2067197.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            804..1595
                     /locus_tag="PWY89_002188"
     CDS             804..1595
                     /locus_tag="PWY89_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067198.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1765..2286
                     /gene="rimM"
                     /locus_tag="PWY89_002189"
     CDS             1765..2286
                     /gene="rimM"
                     /locus_tag="PWY89_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2067199.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2276..3037
                     /gene="trmD"
                     /locus_tag="PWY89_002190"
     CDS             2276..3037
                     /gene="trmD"
                     /locus_tag="PWY89_002190"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2067200.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3173..3520
                     /gene="rplS"
                     /locus_tag="PWY89_002191"
     CDS             3173..3520
                     /gene="rplS"
                     /locus_tag="PWY89_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2067201.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(3652..4788)
                     /locus_tag="PWY89_002192"
     CDS             complement(3652..4788)
                     /locus_tag="PWY89_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2067202.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(4904..5470)
                     /locus_tag="PWY89_002193"
     CDS             complement(4904..5470)
                     /locus_tag="PWY89_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HDL2067203.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(5606..6259)
                     /locus_tag="PWY89_002194"
     CDS             complement(5606..6259)
                     /locus_tag="PWY89_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067204.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(6309..6737)
                     /locus_tag="PWY89_002195"
     CDS             complement(6309..6737)
                     /locus_tag="PWY89_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2067205.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(6742..7116)
                     /locus_tag="PWY89_002196"
     CDS             complement(6742..7116)
                     /locus_tag="PWY89_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067206.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            7420..7605
                     /locus_tag="PWY89_002197"
     CDS             7420..7605
                     /locus_tag="PWY89_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067207.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            7618..7866
                     /locus_tag="PWY89_002198"
     CDS             7618..7866
                     /locus_tag="PWY89_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067208.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            7897..8067
                     /locus_tag="PWY89_002199"
     CDS             7897..8067
                     /locus_tag="PWY89_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067209.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(8169..8627)
                     /locus_tag="PWY89_002200"
     CDS             complement(8169..8627)
                     /locus_tag="PWY89_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067210.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            8795..9112
                     /locus_tag="PWY89_002201"
     CDS             8795..9112
                     /locus_tag="PWY89_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067211.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            9045..9524
                     /locus_tag="PWY89_002202"
     CDS             9045..9524
                     /locus_tag="PWY89_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDL2067212.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            9541..9717
                     /locus_tag="PWY89_002203"
     CDS             9541..9717
                     /locus_tag="PWY89_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067213.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            9974..10405
                     /locus_tag="PWY89_002204"
     CDS             9974..10405
                     /locus_tag="PWY89_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067214.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            10411..10728
                     /locus_tag="PWY89_002205"
     CDS             10411..10728
                     /locus_tag="PWY89_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HDL2067215.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            10847..11533
                     /locus_tag="PWY89_002206"
     CDS             10847..11533
                     /locus_tag="PWY89_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2067216.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            11508..12857
                     /locus_tag="PWY89_002207"
     CDS             11508..12857
                     /locus_tag="PWY89_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2067217.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            12848..13321
                     /locus_tag="PWY89_002208"
     CDS             12848..13321
                     /locus_tag="PWY89_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2067218.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            13296..13823
                     /locus_tag="PWY89_002209"
     CDS             13296..13823
                     /locus_tag="PWY89_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HDL2067219.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            13838..16150
                     /locus_tag="PWY89_002210"
     CDS             13838..16150
                     /locus_tag="PWY89_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2067220.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
BASE COUNT         5433 a         2799 c         3283 g         4722 t
ORIGIN      
        1 aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa atcgaattta atcaagctgt
       61 aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac
      121 aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg ggttctaaaa
      181 agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat ggacgtttca
      241 tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt ttaaaagaag
      301 acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc
      361 tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag aaataaggtg
      421 accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc tagtcactta
      481 tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt acaatctaac
      541 agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat
      601 ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat
      661 cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc agcttttttg
      721 ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag cgtatcctta
      781 taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca tttgctctat
      841 tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta tcaagcagta
      901 tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc accactgaac
      961 aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg aaagaacaac
     1021 agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc aatatcgcag
     1081 cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt cccctcgggt
     1141 tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat caatatgggt
     1201 ttgcttctag tcaagaaaca atccaagttt tgcagccatt tgagatcgac acaaatggtc
     1261 agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca ggttcaagtt
     1321 ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat atagaaggcc
     1381 aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac agtctaccag
     1441 cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat cgagccgtag
     1501 aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat cctatgtctg
     1561 cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat
     1621 catttaactc caagtcacag gaagtaatca aaaatagcac gccgcatttt tgcataattt
     1681 tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa aataaaacag
     1741 acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa aatcgtaaac
     1801 acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc agaagaacgt
     1861 tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt ggaattaaca
     1921 gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg acatccttct
     1981 atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga caactctgta
     2041 gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt attagaagat
     2101 ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc
     2161 gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt cgtcaaagaa
     2221 gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat tgacgatgaa
     2281 gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg aatccatcat
     2341 cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc gcgattattc
     2401 agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag gtatgctgct
     2461 caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac cggatgtcaa
     2521 aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa tggcagaaga
     2581 attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat atgatgaacg
     2641 tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta ctgggggaga
     2701 attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg tgttagggaa
     2761 tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc cccaatatac
     2821 tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg gaaatcataa
     2881 attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac gtcggcctga
     2941 tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag aaatcaagaa
     3001 agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat cttctttaca
     3061 gtaggttgat aatatgttag tattttatgg tgagtgcaaa agcacttaac tattacgata
     3121 ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa ccatgaatcc
     3181 attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt tccgccctgg
     3241 tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta tccagttatt
     3301 tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata cagtacgtaa
     3361 agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac gtgttgcaaa
     3421 aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt
     3481 acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa tcatcgttat cagacagaga
     3541 aagaaccctt ggtattcaag ggttcttttt ttattatctg tttttttata ttaacgattt
     3601 ctatttggta catttttgat taataaaaag gggcaaaaaa ggggcaacaa gttacagttt
     3661 ttctatatct tctttcaatt tttcttttac tccttttgtc acatgcaaat aaattttttc
     3721 agtgatatca ctattttcgt gaccaacgcg atcctgaatg gcatacagag gagttcctag
     3781 ttccgctaat tttgaaatat gggtatgtct aaaaatatgt gaactaagtt ttttatcaat
     3841 cttcatgtca gctttatgac ttctcaaata ggtgttaatt gctgttagtt ggaaaggagt
     3901 tcctttcgtt gtttgaaata gaaattgacc atttggattt aattctagaa gctcattgta
     3961 aatagctatc gctttttttg gtaaatcaat ttctcgcatt ccggcagctg tttttgtaga
     4021 atcagatttt ttcatatcag ctattgaacg ttctctatac atcatcgtcc catttataac
     4081 tactgatgca ttattattgg taatgtgtac atcatcttta cttaatgcga ttgcttcacc
     4141 agacctcatc ccagtcaaat atagccactg gaaaaggaga gaatatcttt tattgtgtga
     4201 agttgtgaaa tctactagtc tattatattc atcatcttct aaaaatttat ctttaatttt
     4261 tatcgttttt gactctcttt tataatcaat aactacttca tcgataggat ttttttctac
     4321 ataacctttt ttcatagcat atgaaaaaag aagattcaat ttggatttaa ttacactgac
     4381 gtacttattt gacaagtcat ctttatatat catgtcttca aaaatattat tcaaatctat
     4441 tgtattaata ccagaaacga tgtaagtctc aggtatcttc ttctttatag tgtttaatat
     4501 attattcgta ggataatatg tagattcctt aacttgtctt ttgtatataa ctaaccattc
     4561 ttcaacaagc tcatgaaatg ttagatcagg cttatgaagt gttttttcgt ttaattttat
     4621 atcaattttt ttatttaact ctagcaatgc cactttctga gtttctcgag atttattttt
     4681 ataagtaatg ctcacttttt ttctttttct agtttttgga tcaatatatc gctcattata
     4741 tttatatact ttttttccgt ttttatcagt tttagtttcg atccacattg ttttcatctc
     4801 ctatttgttg ctataatagg aatagataag taagccgatt atagcaggtt tatttttcat
     4861 cacgtccaca aactttggcg agggaggggc gtgtttttgt tagttattgt ggtaggttat
     4921 ctaaagcgta ttgtgcttgc tcatttgtaa aaccttcaaa tataagttga tcgtacaatt
     4981 gcgaatctga cattgatgcc caatcatcgt aactttccgc tttagccaaa gcttgttcgt
     5041 tgtaatcgac atcaacatta gccagcgcat aatcgattgc atcacttgga tattcttcaa
     5101 actctagttg ttcacgcaaa ccttgttcag acataccagc ccaacctaga tagctttcag
     5161 ctttgctcaa tgcatttctg tattcacgag gaacactttc ttcttgagta acttcttcta
     5221 tagtagtttc agtagtttca gtttcttctt caacgactgt agattctgct accgattctg
     5281 cagaagacac ttctgtcacg ctttctttag ttgtttcagc gacggtattt ttagcatcat
     5341 cactgcctcc ttgcgaacct atagcaataa acaccacaac aaccgctaac aaccaaaacc
     5401 atacccgttt ataaaacggt ttcttaactt tatacatttt cccatcttga cccataactt
     5461 tttttgccat ttaaatatcc ctcgcttctt gttataatat atttgtgatc tcagaaatga
     5521 ggtatgagtc cgtgttgcag cacgggcttt tttctttata acttttttag agattatagg
     5581 caaaatagta gggcataaaa atgtattatt gaattccgta tttagaaaat cctaattgaa
     5641 cttcaccgga agtcttttgc tgtgttgtac gcaatgcttc ttcttcagac attccattct
     5701 gtactttcca tgcaacaggc gacatcccgt atttgttaac aaaatcagta agtgataaag
     5761 tgtcagcgtc ttgctgagcg cttgtttgtt ggtcttctgg attctgttga gatgctgctt
     5821 gttgttcttt ctgatcttga ctgataatat tgccagcatc atctgtagtc aatccatttt
     5881 cataaagggc cacgccgaaa gcttcccact ctttgttgga ccaatttgca cgatcagctg
     5941 gagttgactg taaagtgcgt tgtttcatct gttcatatgt ttcttcttga ggtgcggttt
     6001 ggattgtact ctgactggag cttataactg ttgggctagg ttccgccgta gcttggttgg
     6061 agcttgtaac tgttgaacta gaatctgttt tagatgtaga tttgctagta gatgaactgg
     6121 tttcagttgt ttcttttgtt ttacttattt ttgtttcttg gttagaagtg gtatctgttg
     6181 attcagcttt tttattattt gaacaagctg aaagtagcag agcagtactt aacaacaaca
     6241 taacgctaac ttttttcatt ttataattcc tctttctcgt tgtaatatgt gtgctaacac
     6301 gggctttttt atataagaaa acgataagcg ctttctggaa gcccgtaaag attctttaat
     6361 tcctcgattt ttttaggata ttgatcatta tcttctttat aaagagaaac aatgagatta
     6421 gcagcaaagc aattagcttc gctttcagat ttgcttctag atgttcttgt tgatacataa
     6481 tagctggata agccacgatg aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat
     6541 ctttcctcag agtctcgcag ttcatcattt aaaaagatta tcgcacgatc tctaatttct
     6601 tgaaactgcc ccttgggatt ctcgataaaa ggaacgtatt gaattttaat gcccatcttt
     6661 tcacaaatat aaaaaggatt agcggactgg tatttccgct tcaactcctc gactaaatta
     6721 atcgtatcca tctccataag ctcacatctt tttgcctttt tctttgtctt ctttcacaat
     6781 atcccagaaa gtcgctatca ggatatcttt tacgcgctgt atttgttcgg gtgtcaatgt
     6841 ttccccacca taagacatat taacatttga gtctagtagt ttatcaagtt caaccacttc
     6901 ctcttttgta gcccacttgg gaacattatt atttcccaat aaataatcag ttgtgacacc
     6961 aaaataatca gcaactttct ttaagttctc agattttggc gaggctttat cccatcttct
     7021 tatttgtcca ttagagatgc ccacctgtct ttctacttct gctatagtca catgcttttc
     7081 gtctgctaat tctttaatct tagtaactaa actcattatt atcaaccttt caaagctgaa
     7141 aagaaaataa atagcttaaa agttatattt ttggttgaca attagctttt aagctagtat
     7201 atttaattcg taagctaaat tgttagctaa ataagagcaa caaaaaactc tactaattta
     7261 aaacattctc tcggtcgcca aacttagaaa tgttatttta gaggcttttt ataagtctta
     7321 tttaactatg tattcataat agcttaaaag ctaataggtg tcaacgattt agctaatttt
     7381 ttagcttaca aattatttgt ttagaaagga gctattttta tgtctgagaa tttagactta
     7441 aaaattcgag cggagatgag aaaaagaaga atgactttca aagaactagc tgcgcttatc
     7501 ggtatttcag gagcttattt atcagatatt ctaaacggca atcgtgatgg aaagaaagca
     7561 caacagcata tcgaaacagt gaaaaaaata ttggacatcc gatagggggg gtaggcgatg
     7621 acaaaactaa aaaaacaaga ttttgtaaaa aaatataatt attctccatc tacttatcaa
     7681 cgtcgaatgt cggaactaaa aaatacagca attttctcag cggcgtatga acgggtcaca
     7741 ggacaagaag tttggatcaa tacagaatta tacgataaat ttttgtcttt caaatcctat
     7801 aacaggttac gcacaagaaa ggtaacacct aaagaattta tcgagaagca tttagttgat
     7861 ttataaaaaa cagaacattt tgagaggtga aggttaatgg gtaaattcaa cagagcatta
     7921 gtattcagcg caccgctaat cgtctatgct ttagggcttt ggggaagtag acaagcgttg
     7981 ataggaacga ttgtgtacat ggtttggatt tttatagggc tggatgaagc tgagtacaaa
     8041 acaaaaaagc cagtcgggag ggactgacta atgagtaagg acgatattgt atttctgtta
     8101 attatgtttt gtgggattgg atcaataata attaatttgt atttgattat taaagaaaaa
     8161 tttttcaatt aaaatgagtc ttttagcttt aacttaaatt ttttttgata attagctgtt
     8221 cttatgacca ttacaagttt ttcaccaggt aatatatctt tcatatctaa aacttctgaa
     8281 aaagcgactg agtagccagg agagattgct tgtgccaacg gtataggaga taaaaggcta
     8341 cttttatgaa tgcttcccat atcaaaacca ttggcgtgat aatgtgaaca attaattttc
     8401 ttaccgttgt aaagatacgt acattcgata attgagtttg gcatttttga tgggtttgca
     8461 attacgccag taagaatcat tcgattactt ctatcatgct taactaaaaa ataggagagc
     8521 gtaatttttc ttttatcttt aaataagtta tagagggata aaaaaaatcc tgaaatagaa
     8581 atgattaaag taatagcgac ttggtaatcg ctcaaaattt ttatcaagta attcaccacc
     8641 tttttactca aattatatca aaaaggagag aagaaataat gcaagaatta gtaattttga
     8701 aaaataaaaa aagacacgaa cgccggcaag catgatcgtg tcagggaaat aaactatctt
     8761 tctatatttt accacagaaa ggaatgaacc gtaaatgcta attgcaacgg atacactaga
     8821 ccgcatcttt ttaaaagacg aatacaaact gcgcaatata gatgcgtcag gaattttagt
     8881 atttgatctt tatgacaatg gaaaaattgg tatctatcaa gcaagtgata ttgaagaaac
     8941 aaaccttgct ttcgaacaaa ttgatgattc tgtggaattg gatttagatg aggcaatcct
     9001 agcttttgaa caaattgcaa aattattaaa ggaggcacaa aagaatggca actctttacc
     9061 aactcagcga gtcatatatc aaagtcctgg aactggcaga acaattggat gaagaaattc
     9121 ttcgcgatac tctcgattcg attaatgaag cgatcgagta taaggcagaa aacttagcaa
     9181 aaatagttaa agaagtagaa gggaaagctg agttaatcga tagtgaaatt aaacgtctgc
     9241 aggaacgtaa gacatcgctt ttgaacaatg ctaagagtat caagcactat ttacaagagg
     9301 aaatggaaaa gactggtaaa acgaagatta aaggtgaatt attcaacatt ggaattcaaa
     9361 ataatccagt atcggtcaat gtaatcaatg agaacttaat tccaaaagga ttctttaccc
     9421 ctgtgcctcc caaattggat aaaaagcaat tgaaggagga actgaagcac ggagatattc
     9481 ctggtgctga actcgtccaa acaaaaggtt tgaggataag atgatgaagg aggagaaata
     9541 atggaacacg aaaacgtaaa agacgctttg aaagctgcta tcgaaatcgc ggaagctaaa
     9601 ggaataaagg ttgacggcaa accagcaact gtccatgaca ttcaaaattt gacgaaagaa
     9661 catctttatt ttatcgccga cttgcttggt ttgtcggaat tgtatttaga caaataaccc
     9721 cacacggaag gagtggctag ggtggttgta ttacaagtaa tagtctgcgt aacattcatt
     9781 tggtcgtgca taccggtttg tatatatatc atgaagaagc ttcgccctga gtcttacaaa
     9841 ttatacgcaa tttacagcat aacagtagct cttgcattta cattgtttgc aatttctagg
     9901 cttccgtttt aatcgtggga atgaattttt aatgaactaa attatctata aagcaaaaaa
     9961 aggagaaaca acattgaaaa acaaactagc aaaaacaaca gcaatcatcg gactagcact
    10021 aggtagcggc gttatcggct acgcagcaag caatgcgttt caagatttgg acacgatcaa
    10081 agcaaatttc aacacagtcc tacaatacgg tcaaacgaaa tcgcaacgtg tgtcagaact
    10141 tgaatcacag ctatccaaca acactcgcac acaggagcag ctgaaagccg aaattgagca
    10201 aatcaaatcg gacaagcaga aggaaattga agccaagcaa cgagaagtcg aacaaaagca
    10261 acaggagatc gctacaaagc aacaggaagc cgatagcttg cgccaacaac tatccacagt
    10321 gcaaaacgac aaggagcagt tagaacagcg cgtgagcgag ttacggcagt atacggatca
    10381 aaaggtaggg gagttgggga aatgaaagag ttgactattt atgacccaaa tatttcatgg
    10441 gctaaacaca caattaaagt ttcttttatg atttggggtt acaaaggata tgttacttac
    10501 aaagtgggcg gtaacactaa gggtttatca ttaatagcta ttgattcaga tgatctatat
    10561 gatgctaatt tcgaggataa cccagttaat ttccgtgacc ttgatgaaga ttggttctct
    10621 atggaactta ctaacgataa aggcgacagt acacttgtag aagatgaatt cgacagacta
    10681 ggtgattata ttgtcggtgt tgaaattatt gcgcacgaac cagagtagga gagtgaatac
    10741 aaaatgatta aaagaatcac tatatcagtt gaagaagata gtggatcaac aatgcagcat
    10801 accgacaatc gaaaaacttt tttaaccaat agaaaggaga attttgatgc agattaagaa
    10861 ggcgacggat atcgaacaaa ccaagggaac atatttgatc tacggtgctc caggaaaagg
    10921 taaaacttca acggctaaat attttccagg gaaaacactg atccttgata ttgatcggac
    10981 ttcaaaagtc cttagaggtg agaaaaatgt cgatatcgta tatatcgaca acgaagatac
    11041 ctggaacgac tggggcaaca cgttggctga tttaactacc aattatgtag gtgtttatga
    11101 caacgtggtt gtcgataatg tcagcgaatt ggaacggtgt attctgtcca gtttaggcgc
    11161 agaaggaaaa aacaacggcg ttccttcgca aggcgattat caatatatgc aatttagaat
    11221 ggtgaacagc ctacgctata tgaaaaattt agacagcaac cttatctgga ccgcatggga
    11281 agaaatcgat ttgtggacgg attcggatgg atcttcttac aacatcgctt tgccgcagat
    11341 caatcgaaag attcgaaaca atattttggg attatgtgac gttgttggtc gattgatggt
    11401 aaaggaagac gatgaacgcg gatttatttt acaagccaca gattcaacct atgccaaaaa
    11461 tcaattggat aatagatctg gctgtaagca aagtgagctg attttagatg gaattgtacg
    11521 actaccagaa tgatttagtc gatcgtgcaa gacaagccta tgttgatggt tacaaggcac
    11581 catgtatcgt atcaccgtgc ggttctggaa aatcagtcat ggttgcagag atcgctaaac
    11641 gaaccacacg aaaaggcaac agagtgttgt tcttggtcca tcgtcgtgaa ttgattgatc
    11701 agatcaaaga tacgtttcaa aagatgggcg tagataatca gcttgtaaat ttcggcatgg
    11761 ttcaaacagt cgtacgacat cttaaaacca tcaagaaacc acaactgatc atcactgacg
    11821 aaaaccatca cgggttagca gcgtcctaca gaaaaatcta tgagtattat tcggacgttc
    11881 cgcgattagg ttttaccgcg acacccatcc ggttaaacgg tagtgggttg ggggatgtga
    11941 acgacttgct aattgaagga gtatcggcta aatggttgat tgagaatcat agattagctc
    12001 cttatgaata ttatgcacct aaattgatcg atacagccga attaaagaaa gcttcgactg
    12061 gagattttac aaagaaatca atggataagg cagtgaagaa tactatttat ggcgacgtgt
    12121 tgaaacatta taaaacgtta gcagaaggtg aacaggcaat agcatactgt cacagtatcg
    12181 atgcaagtaa acacactgct gaaatattca acgatgctgg ttataaagca acacatatcg
    12241 atgcaaaaac tccaaaagat gaacgtgcag atattatcga agcgttcaga aatcatgaaa
    12301 ttaaaatcct atgcaatgtt gatttgatcg gagaagggtt tgatgttcct gattgttcga
    12361 ctgtaattat gttgagacct actcaatcgt tatcattgta cattcagcaa tcgatgaggg
    12421 ggatgcgtta ccgaccagat aaaacgtcaa tcatcattga ccatgtgggt aatgtgaacc
    12481 gttttggatt gcctgatatg gatcgtgaat ggagtttaga tacaaaaaag aaaacaaaaa
    12541 ctgatagtga tttatctatc gttcaatgcg cgttttgttt tggtgcttac gaacggccaa
    12601 aaggcgataa tatttgtccg tattgcggag agttgcagcc gattgaagaa cgaaagtcag
    12661 agttagaaat cgatgaatcg gctgaattaa tgaaagtcgg agaaacgaaa attactttga
    12721 attttgagaa caataaatat tacaacatga cggaagatga agcagaaagt atagaggact
    12781 tatatgccat agcaagagct aaaggattta aacctggttg ggcttatatg gctgctaaga
    12841 ggaaggggtg gttgtaggaa tgggcggtat ttttggtaaa aaagcagaag atgttcaagg
    12901 ttcaataata aatggcgtaa caataatagg tgatactgga aaaagagata gttcaggcaa
    12961 tcaaattgta atcgctagaa ataaaaataa cgaagttttt gaatcttctc tatctaatat
    13021 aaaaagaggt gttgttaacg ggacaggcga agagagtggc tacgttaatt taaaaaaagc
    13081 taatgaaaaa taccatgttg aaggaactta cgttaaggct ttgtctgtcg gtggaacaaa
    13141 aggcgttaat tgggataaac gaagaaataa atggaaagca cgattatatt tcaaaggaga
    13201 atatgtctta caaaaggatt tcaacaccaa agaagaagca atccaagcac gacttgcagc
    13261 agaagaaaaa tatttcaaac ctatcttaga aaaatatgaa aataaaaagg agaatgatta
    13321 acatgacagg atttaactta gacttttcaa aagcgcaaca agggaacgaa atcaaagacg
    13381 gtacgtacga agtggtcgta aacaaagctg tagaaaatgc aactaaatca ggcgcagaat
    13441 ttatcgatat cgatttgatc gttcgtaacg atgtggatca accgttccaa aataaacaca
    13501 ttttcgcaaa aatctggaaa gccaaagcta ccggcaaata taacgaaggt atgatcatgg
    13561 caattgcaca agcgctacag ttggaagacg gaaaatcata taacgggttt gatgaattgc
    13621 tggcggactt tgttttgaaa accgcctcag tacgcgtgaa aactgaagag tcaaatggat
    13681 ataaaaacgt caatgtgaag tcttgggata aaacgaacac acggggcgta atgaatcacc
    13741 aattcaaaaa tggtgatgaa ccatccttcg gaccggaacg ttcaagagcg acaacggtta
    13801 gaaatgacaa tcttccattt taattgaggt gtaatcaatg tatgaaaaaa ttccaaaaga
    13861 gcttaaaaat ctcaaacaat ggtgtgttta taagctagtt tgggatgaaa agcggcagaa
    13921 gcacacgaag atcccttata acgccaacaa cgggcataaa gccaaatcca atgacgagag
    13981 tacctggtct gattttcaga ccgctctcgc tgctattgat cactacggaa tgagtggact
    14041 ggggttcttt ttcaaaccac cttatttcgg cattgatatc gataatgctg aaggtgaagt
    14101 tgagcgattt aaatcaggtg atatagaaga aaatattatc tatgaattta tcgaaagcat
    14161 gagatcctat gctgagtatt ctcaatcagg tactggcatc cacattattg ctcgcggaga
    14221 attgcccggc ggaagacgca ggaaaggtga cgtagaaatg taccagaacg gccgattctt
    14281 cgttatgact ggaaatgccg catcaaaata tttagaaata actgagccga atccaaagga
    14341 tatcaaacgc ctatatgatc gctacgttgg agatgaaaaa atcatccaat tcaaagaaga
    14401 aaatccgctg atgaataccg ttgatttacc ggttgaagaa attatcaaac gtgctgaatc
    14461 gtcttcccaa ggggcaagat tcaaagtctt tatgaacggc ggttgggaag ctttttattc
    14521 ctcgcaatcc gaagcggata tggcgttcgc caacgacttg gctttttgga ccggtagaga
    14581 ctttgaaaaa atggatgaga ttttcagagc gtctgcaatg atccgtagca aatatgacca
    14641 aaaaagggga tcggcaactt acggagaatc tctactaaat aaggcaattg ctgatacaaa
    14701 ttccgtctac aaccccaaac ggaaatctga ttttaagatt tttatcaaag accaggaaca
    14761 gccaaaagag gaaaaatact attcgtacga cgacaccggc aacgcggatc ggtttactga
    14821 tatctacgga acgttagtca aatattcgta cattgataaa tcttggtatt actacgatgg
    14881 gaaagtttgg ctacaggaca ataccggcga agttcggaaa atgatcgata cgacagtaga
    14941 catcatggga aaagaaccgt taacgatccc tgaaggcgca gacgatgaaa ccaaagaagc
    15001 tttgatgaaa gcgaaagaaa aacacgtcaa gcgttctcgc agcaatgccg gaaaaaacgc
    15061 tatgatggac gaattgaaac atcgactatc cgtattgccg gaagagttcg acaaagataa
    15121 aacgctgttc aacacacaaa gcggctatct gtctttgcat gacggtcttc tacacgagca
    15181 tgagatagac aaaatgttta cacgtgtttc aaatgtcgag tacacagaat cggtcgattg
    15241 tcctatgtgg gaagaattta ttaatcagat ttttgacaat gatcaagaat taatcagata
    15301 tatccagaaa tgtgtcggat attccctgac cggatctacc agagagcaat gtatgttcat
    15361 cctctacggt catgggtcaa atggaaagtc ggtatttttg gaaattattt ctgaattgat
    15421 gggaaactat gcgatgacta tgcaggcaca aacgatcatg gtcaagcaaa gccaatcatc
    15481 agcaaacagt gatattgcac gattgaaagg tgcgcggttg gtcacttcgt cagaaccgaa
    15541 tgaaggcgtc cgactggacg aaggtctggt caaacaattg actggtggtg ataaagtcac
    15601 ggctcggcac ctttatggaa aagaattcga gttcgaacct gaattcaagt tgtggctggc
    15661 aacgaaccac aagcccatca tccgtgggac cgatgacggc atctggcgac ggttaaattt
    15721 aattccattc actgtccaaa tcccggatca caaaaaggat aaaaatctga aatttaaatt
    15781 gcagacggag ttgcaaggga tccttaaatg ggcaatcgat ggttgcctgt tgtggcagag
    15841 agagggcttg gagaaaccaa agtcggttgt tgcagcgagt caggattatc gaaacgaaat
    15901 ggatcaaata ggtacattta tcgaaacgtg ttgcgaaaca ggtccaggac taaaaatatc
    15961 aggcggagaa ttatacaaag tttatcgtga atgggcatca gataatggcg agcatacttt
    16021 cactaacacg aagtttggca gagagatttc aaagaaatat agcaaagaaa aagctggtgg
    16081 atttatggtt tataaaggaa tcacattaaa accaagaaaa tacgataacg ttagagaact
    16141 ttttaaataa atctggaggg ttctggaggg ttgagaacga aaccctccaa cccttagaaa
    16201 cgttgatata tcagtattta tattatattt ttctttc
//
LOCUS       DANKBF010000062        16203 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000062 DANKBF010000000
VERSION     DANKBF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            226..894
                     /locus_tag="PWY89_002211"
     CDS             226..894
                     /locus_tag="PWY89_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL2067221.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="PWY89_002212"
     CDS             complement(1094..1324)
                     /locus_tag="PWY89_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067222.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="PWY89_002213"
     CDS             complement(1451..2833)
                     /locus_tag="PWY89_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2067223.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="PWY89_002214"
     CDS             complement(2989..4176)
                     /locus_tag="PWY89_002214"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2067224.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="PWY89_002215"
     CDS             complement(4183..5214)
                     /locus_tag="PWY89_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2067225.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="PWY89_002216"
     CDS             complement(5230..6684)
                     /locus_tag="PWY89_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2067226.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="PWY89_002217"
     CDS             6859..7764
                     /locus_tag="PWY89_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2067227.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="PWY89_002218"
     CDS             7855..8532
                     /locus_tag="PWY89_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2067228.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="PWY89_002219"
     CDS             8529..8699
                     /locus_tag="PWY89_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067229.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="PWY89_002220"
     CDS             8813..9709
                     /locus_tag="PWY89_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2067230.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="PWY89_002221"
     CDS             complement(9764..11134)
                     /locus_tag="PWY89_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2067231.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="PWY89_002222"
     CDS             11372..12118
                     /locus_tag="PWY89_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2067232.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="PWY89_002223"
     CDS             12115..12954
                     /locus_tag="PWY89_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2067233.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="PWY89_002224"
     CDS             12955..13905
                     /locus_tag="PWY89_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2067234.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="PWY89_002225"
     CDS             14046..14798
                     /locus_tag="PWY89_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067235.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="PWY89_002226"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="PWY89_002226"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL2067236.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5257 a         3018 c         3250 g         4678 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
    16141 aataaaaaaa catagaaaaa cgattacttt gcttatacaa caaggctttt atggttttgt
    16201 atc
//
LOCUS       DANKBF010000063        15741 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000063 DANKBF010000000
VERSION     DANKBF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(24..722)
                     /locus_tag="PWY89_002227"
     CDS             complement(24..722)
                     /locus_tag="PWY89_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL2067237.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="PWY89_002228"
     CDS             complement(715..2067)
                     /locus_tag="PWY89_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL2067238.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="PWY89_002229"
     CDS             complement(2290..3690)
                     /locus_tag="PWY89_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2067239.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="PWY89_002230"
     CDS             3851..4588
                     /locus_tag="PWY89_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2067240.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="PWY89_002231"
     CDS             4713..5192
                     /locus_tag="PWY89_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL2067241.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="PWY89_002232"
     CDS             5189..5956
                     /locus_tag="PWY89_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067242.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="PWY89_002233"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="PWY89_002233"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2067243.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="PWY89_002234"
     CDS             complement(6859..7461)
                     /locus_tag="PWY89_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2067244.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="PWY89_002235"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="PWY89_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="PWY89_002236"
     CDS             8142..8726
                     /locus_tag="PWY89_002236"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2067245.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="PWY89_002237"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="PWY89_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2067246.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="PWY89_002238"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="PWY89_002238"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2067247.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="PWY89_002239"
     CDS             10627..10806
                     /locus_tag="PWY89_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067248.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="PWY89_002240"
     CDS             10828..11853
                     /locus_tag="PWY89_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2067249.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="PWY89_002241"
     CDS             12028..13272
                     /locus_tag="PWY89_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2067250.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="PWY89_002242"
     CDS             13425..14372
                     /locus_tag="PWY89_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2067251.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="PWY89_002243"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="PWY89_002243"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2067252.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="PWY89_002244"
     CDS             complement(15274..15516)
                     /locus_tag="PWY89_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067253.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="PWY89_002245"
     tRNA            15637..15709
                     /locus_tag="PWY89_002245"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4374 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt t
//
LOCUS       DANKBF010000064        15445 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000064 DANKBF010000000
VERSION     DANKBF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(1017..1358)
                     /locus_tag="PWY89_002246"
     CDS             complement(1017..1358)
                     /locus_tag="PWY89_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2067254.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1358..1588)
                     /locus_tag="PWY89_002247"
     CDS             complement(1358..1588)
                     /locus_tag="PWY89_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2067255.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1875..2213
                     /locus_tag="PWY89_002248"
     CDS             1875..2213
                     /locus_tag="PWY89_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067256.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2418..2657)
                     /locus_tag="PWY89_002249"
     CDS             complement(2418..2657)
                     /locus_tag="PWY89_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2067257.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2633..2980)
                     /locus_tag="PWY89_002250"
     CDS             complement(2633..2980)
                     /locus_tag="PWY89_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2067258.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3712..4293)
                     /locus_tag="PWY89_002251"
     CDS             complement(3712..4293)
                     /locus_tag="PWY89_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2067259.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4476..5102)
                     /locus_tag="PWY89_002252"
     CDS             complement(4476..5102)
                     /locus_tag="PWY89_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2067260.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5124..6455)
                     /locus_tag="PWY89_002253"
     CDS             complement(5124..6455)
                     /locus_tag="PWY89_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL2067261.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6610..7011)
                     /locus_tag="PWY89_002254"
     CDS             complement(6610..7011)
                     /locus_tag="PWY89_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2067262.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7097..7303)
                     /locus_tag="PWY89_002255"
     CDS             complement(7097..7303)
                     /locus_tag="PWY89_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067263.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7398..8900)
                     /locus_tag="PWY89_002256"
     CDS             complement(7398..8900)
                     /locus_tag="PWY89_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL2067264.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8918..9373)
                     /gene="argR"
                     /locus_tag="PWY89_002257"
     CDS             complement(8918..9373)
                     /gene="argR"
                     /locus_tag="PWY89_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2067265.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9471..10088)
                     /locus_tag="PWY89_002258"
     CDS             complement(9471..10088)
                     /locus_tag="PWY89_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL2067266.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10309..11682
                     /locus_tag="PWY89_002259"
     CDS             10309..11682
                     /locus_tag="PWY89_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2067267.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11757..12869
                     /locus_tag="PWY89_002260"
     CDS             11757..12869
                     /locus_tag="PWY89_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2067268.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12941..14578)
                     /locus_tag="PWY89_002261"
     CDS             complement(12941..14578)
                     /locus_tag="PWY89_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2067269.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14746..15354)
                     /locus_tag="PWY89_002262"
     CDS             complement(14746..15354)
                     /locus_tag="PWY89_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2067270.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4277 a         3169 c         2693 g         5306 t
ORIGIN      
        1 taaccaacag caaaaaatac aatgaaaaac aaacttggaa ctaatgtatc attaataaat
       61 gttgaaaaat tcagcaactc attactgtat gattttagca gcccaaccaa catataccct
      121 ataaaagcta ataagatggt taggaatcct ttagaatttt tactcattca tagacacttc
      181 cttaaataac ttttattata gaaggtattg taaataataa tattgacaaa aagtgactca
      241 aaataaaaaa aaccttacat tatactggaa tgaactgtat tgatagtgta cctaaaagct
      301 ctactgacat aagatcgtgt aatcggtaaa gtaattttat taattatacg ggatgtaatc
      361 aattagagaa taaactaatc ataaaaggta cttttacatt cataattgtc gctttgagat
      421 tctactttgg ttcactatct gacccaattg cacattttcg atcattacaa gtattggtgt
      481 cgttagaaca actaaataaa aactagcggt agatttttca gaatctatgc taatttctgt
      541 atggatgttc cagtgatggc aaatttctct tttcattgtt tttcttgtcg catgatatgc
      601 ctttttgtgt tattcagtta aactatagaa attctttcct agacagatct tgatcccaaa
      661 ttttacgttt ttgagcatcg atatagttta aaatgacatt actcatatct aaagagttta
      721 gattttttag gactaaaact gtcagttgtt ctttttcttc tttctgttaa agtattgtcg
      781 cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa gttttgtgac acaaaaaaac
      841 gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat gctatatatt ctttagttta
      901 agatacggaa attaatgcca tcgaccacac tagtagaatg aacgacattt acttggcctt
      961 tttttaattt cgttatgttt agcatataaa aatattatag agtttactta caatgcttaa
     1021 aacatatact gtataatctg atcaatttta gccatgtctt gcaatggtaa gttttctatt
     1081 tttttgagtt ttcgttcctt aaaatcaaga gattttaatt gtgtaataat tacttgtcct
     1141 tttatatcta gctcttcagg taatgagtaa cgtgtcggta aattctttat agtggaagta
     1201 attggacata tgaccgaaaa cattgtttta cggttaaatt cataacgaga gacgactagt
     1261 cctggtcgtc ttttttgaat ttcttttcct acggagggat caaaatcaat ccatacaata
     1321 tcaccttttt taggaatata aattctttct tctgacacta taaaatttct ctcccttcag
     1381 gagttaaatc ttcccattcg tctttctcat aatactcggc ctcttcccca ccactaaatg
     1441 gatcttcaat ttttggaact aacgtaattg ttccatcatc agaatacaca acgatatatt
     1501 cttggttttc tgaaggtttc tttccgttat cagaaggtaa agttaccact acagaacttc
     1561 cttgtaatct agattttgta acaagcatta tcatcactcc cttaataaaa gtatactata
     1621 taacactaag taacgcaaaa ttcccctttg taaactatct agaaaattgt tttgtttaaa
     1681 gtatatctta ttggatataa atagaaataa attgtagcca gttcattttt tctttaacta
     1741 aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa gctacccaaa tactatagct
     1801 aaccccccgc taccttcaat tttgcagata aaacgctttg ggagaaaatt tcaaagacaa
     1861 caggccatcc tatcatgccc gattataaac aaaaagctgg ctattccttt tgctctgaca
     1921 aatttcaatt gggaaagttc acgaaactag caggtgaaga agtttcaact gttcggatca
     1981 aagaatgccc gcttcaaata gaaacattgg ttcaacgaat ttcaactaga caagactttg
     2041 ctattgtaga acgtgagatc gtagctattt ttgtagaaga agggatttta catgatccaa
     2101 ctcatatcaa tgtggataaa tggcaacctc ttatctataa attcagagaa tatacaactg
     2161 ccaaccagtc gttaactttg aattttagat tccaagaatt caaagagtca taatgataaa
     2221 catcgttagg tataaataaa aaagactcgc agcacgagta aaaaacaaat agaagctata
     2281 cattagaaag gtcgactgtg accctataga tgcagtatat gcagcttatg agcatttctt
     2341 ctgttctctt tgtttttggg atctgtgctg aagtcttttt cgttttttgc cgctctttct
     2401 ttgaaaaatc gattatgtta taccttctcc attactaatt caggcatctg ctgagtaatg
     2461 atttttcgcg caaaagaaat atctgttacc caaggttcga tttgttcttc agtcaagaca
     2521 agcggcattc ggtcatggat tggtgcgact gtgcggttag gttcagtagt cattatgatt
     2581 gattctgcca ttttttcatc ctgatggcta cgataaaaac cgccaatata cactattttt
     2641 tccttatttg aaaacataaa tttgtctttt tcttcattcc actcattggg aaaacacaac
     2701 gattttgcaa aaatggttga tgaaaggtct ttttttcttc tactgtctct gcacgggcat
     2761 taatcatcaa ttgtcctttt ttaaatccgg taaatcccca cttcatgatt tccggagtga
     2821 ggatgttttc ttcctctttt cttaacgtca caacaagatt tgacggaaaa acttctccag
     2881 ctgctaattc tgctgcctgc ttcggttgtt tcgtctgggc ttctcgataa taattctgaa
     2941 ggagctctga ctctagatca aaaaaataac gaccacacat cctaatccct cccaatcttt
     3001 ttttctatca tacactgcaa caagaagttc gcaccaacaa aacgctttca atttttgtag
     3061 aactaaaaaa gttgtgacaa gtgacaaact ctccagtgaa tggtattctc ctgccaactc
     3121 ctcggtcaca agtcacaata ttcaacaaac atgagaagct gctttttgaa attgttcctt
     3181 gtgactcgga gtgaaacggc aggcttacga cctctgggta aacggtgttc aaaagccagt
     3241 ttctcggtat taagccaaat ttatgcaaaa atgtagaaag ctattttcac aaaatttttc
     3301 ttaatactcg aaactaagcg actttttcac aacctcttcc ttaaacggtg ttcaaaagct
     3361 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gcagctattt ttatttggct
     3421 gttcttatta cttgatgcag ggcagctttt tcacaacctc tccactaaac ggtgttcaat
     3481 cagctaacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt
     3541 gtttctctaa tactcaggtc aaagcgctga tttcacaacc tctaatacac ggtgttccag
     3601 aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta
     3661 aattccttct tatttcttga agctgaccac ttctgtcaca acctctaatt attaaagaac
     3721 cttttgcgaa gttaacaatc tttctttgat caattgaaaa ttctgcctaa gtagtgatag
     3781 atcctgtgtg actttcaaca ataagatact tccctggatt tcagcaatca acgctttggc
     3841 ttgacggtct gcttcttcat cggacagttt ttcttcagaa agtgccagga tattttgttt
     3901 cgtatacttg acccattgat tgactaattc tagtagcaac agacggtatt cttcatccac
     3961 tgtactcaac tcaataatca aattacctac aggacagcca tgataagtga tctgtgtctc
     4021 atgatcagcg aaaatccagt ccaacatttc ttctaatgct tcttctggct gttttccagc
     4081 atttaagatg tcttcgaaca attctgtctg ccatacgtcc atatgcgcat ggatcaccgc
     4141 tagacaaact tctttttttg aagaaaagta ataataaaat tgaccttttc ctgtatcaga
     4201 agctgctaga atatctcgga ttgacgtttc atgatagcct tttttataaa taagatcttt
     4261 ggtcgcttcc aagatctttt cctttgtttt caatgatatt ctcctttttc atttctcata
     4321 gtcctatatt atacagtaaa ttcatcattt tttcttctta atcgtttaaa aaagactgtg
     4381 ccaaattatc tgacacagcc ctcactataa aactgttctc atatagctag atgttcattg
     4441 attcaagaac tgcgaggcca aaagcaattc ctatcttatg atagttgctc taatacttcc
     4501 tctgttgtca tcacttgatc tgcaataaag ccaaagttcg tcaacgctgc atccattgct
     4561 tcttggccag gagcaccaat tgcatctgtc gctaccacaa cattgaatcc actctctacc
     4621 aattcacgca tatgggaatc gctacacaag tttccgttca ttccaccaag aattactgtg
     4681 tcgattccat tttttctcaa ttggagcgcc aaatcattgc tttctggtcc aaagatttta
     4741 tgaggagagc tgatgatcgt attttcatcc aaataaggtt tcaattcttc gataaaatcg
     4801 gctcctgaac cttcttccac tgattcatat tgactttttc tagcaaacat ctgctgctca
     4861 agcatcatat gttctcctgc accgccaaat tgccatttat ggtcatgtgg gtaaaaataa
     4921 tgaggagaaa taaagagttt gtatcctttt tcttttgctt gttttaccat ttcaatcata
     4981 ttttcgatcg tattgtattt ttctaagata tcttttgtca aaccaaagcc accgcctgct
     5041 ggtttcaaga actcgacttg cgggtcagtg agaactactg ctgtattttt attattgaac
     5101 atggtggatt ctccattcgt tatttatttt aatacatcat ttagagcttc actcattgtc
     5161 ggatgagaat agatttgatc tctcaataac gtataaggaa gttttccatt catagctagc
     5221 gaaatcaagt tgatgacttc atgagaatct tccgcataga tacttgcccc tagaatcaag
     5281 tcagtttcag ggtcgactaa aactttgaat acacctttag gatcttctaa tacttgtgct
     5341 ttagggacac cgcttgtcat atacttgaat aatttgtaat tgacgccttg tgctttggct
     5401 tgtttttctg ttaaacctac attggataaa gtcggagtga taaatacact gtaaggcaca
     5461 gctttccggt tattcgttgt tcgtttattc tcgcccttca actgatccat aatgattcga
     5521 taatcatcta gagagatata agtgaactgc aatccgcctt tgacgtctcc aatcgcccaa
     5581 atgttgtctg catctgtacg aaggtattca tccacagcga ttgctccgcg atctgttagt
     5641 ttaacatctg tattttctaa tccaagtgat gctgtacttg gttttcttcc agttgcagcc
     5701 aaaacttttg acgcttggat tgttacttct tggccatctt ctgtaaaggt gacggcagct
     5761 gttgattctt ggtctgctac ttgatctaca gatacaccta gatggaattc gattcccgca
     5821 tttgtcatgt cttctaaaat catttgcgcg atatcttcat cttcacgtgg aagaaattca
     5881 gaatgggcat caagaacgac aactttagat ccgtattcat tgaacattga tgcaaattcc
     5941 aagccaatat atccagcccc gatgatcact aatttttccg gtaattgttc ctgatccatc
     6001 gcttgcgtag aatcaatcaa gtagcgactt gtattcaatc ctttgattgg taaaatgatt
     6061 ggttcagaac cagtattgat gaaaatccgt tcgccttcaa caatcacttt ttcaccatta
     6121 ttcgtcactt cgatttgatg gttagaaaga aaacgtgctg taccgtccat cactgtccca
     6181 gttgcctcat cgctgatcat atgataattt ttggcgcgaa gcatgcccgt caatttttct
     6241 ttccgagcaa cagcctctgt aaagtcgatt cctctttctc cattgaaaat caatgattta
     6301 gaaggaatac agccgatgtt gatacatgtt ccgccataca tacgtgttga cttttcaacg
     6361 actaaaaccg attcaccttt tgaagctaac gccttcgcta aagtcttacc gccctttcca
     6421 aaaccgatca caatattttg atatttttcc atcatttttc cctcgcttct tttttaccta
     6481 ccgattggta caaaaatatg ataaccgata attgtctatt ctccaaatca tctgctttat
     6541 ttgtcaaaaa aaaccagaca aaaagcggtc ctttcacgtt tttagtctgg ttcgatttcc
     6601 tttattcttc taaacaatcg ttacttctac atcgatattt cctctagttg ctttggagta
     6661 agggcagaat tcatgcgcat cttctgcaat tttttgggct tcttcttcgc tgatgccatc
     6721 gatatgcact tctaatacta caccgatctg atagccgcca tcatctaaat tataaagaga
     6781 gacacgtgct tttactgtgg actctgctgt cattcctgct tctttgatca tatgttgtac
     6841 cgccccattg aaacaagaag cataacctgc tgcaaataat tgttctggat ttgttccttc
     6901 tgcatggatt cctggaggag tgattttcat atgcatcgag ccatttggtg cctcgacttc
     6961 tccattgcgc ccgccatggt tgatcattgc tgtttcgtat gcttttttca ttgtactgct
     7021 cctttcgatt gaccatttgc tacttggcta taattatagt gtatgacaag taaggcgctt
     7081 tctacaaaaa ttctgcttat tcgaatacca gaatgccgtc ttctactatt tttccaaaaa
     7141 acaaaaaaat actactcgtg aacaacagcg tcatatttgt tccaaaataa atagcaatct
     7201 tttctacata atcttttata tgattgattt taagatacat atataccaga aagttgaccc
     7261 cataaaccaa tactgttaaa aaaataacca tattcgttac cattttttcc tccttttgct
     7321 ggtctcccta ccagcactcg atttaaccat accacttttt ttgccgtcca agcaactagc
     7381 ctttcaaaaa gactggttta ttctgctgtt aatgattcaa gatcagaaaa atgtccgaat
     7441 aattttgcta attttactaa cgccaacttg ttctttccga attcatcatt ttctatctgt
     7501 aatactagta acggctcatg taagcttaaa cggatcaaaa accagccgtt tccgtatggc
     7561 tcttctagat catatcgaat accttcttca ttttccgggt cgaagttcat ccctttttct
     7621 tcttccaaca gtcgcttcat ttcttctaat atcttgtttc cttgttcgcg tggttcatcg
     7681 ctgaacaatt ttaatcggat ttcttgtact tctacgggct gtttcaactc tttgatttgt
     7741 tcatctaatg tgataccttt tctttttaat tctggcaaaa gcatcaggat ctttgcgatg
     7801 acatatgcac catcatccag aaaatagttt tccttgaatg cagcgtgtcc gcttgtttcg
     7861 atggctagct gtgtatcttt cccaagacgg ttaagttcta atgctttatt gattacattt
     7921 cgatagccgg aaatataacg aacttggatg cctccttttt cttcaatgaa tctttgtaga
     7981 tgagaagaag taggggaatt cgtcacgatt cttgttcctg gatgctcttt taatacgatt
     8041 gtacttaata ctgcgatcaa attatttcga ttgatcactg ttccagaagc actgacaact
     8101 gcagaacgat ccacatctgt atcgaaaatg atcccaagat ctgcttcatt tgctaaaaca
     8161 gcctgcttga tactttccat cgcttcttta ttatcaggat ttgggatatg attaggaaat
     8221 cggccatctg gttccaaaaa ttgggaacct gttgaatccg cacctaagct attcaatact
     8281 ttttcagcaa agaatccacc agcaccattt ccggcatcaa caatgatttt ccaaccattt
     8341 agtggtttgc tttctagtgt tccaatcccc tttttaattt ttgtgactaa atcttgcgca
     8401 taaggtgtca aaaggtctgc tttttctacg cttccagttt gttcgctttt tattttttgc
     8461 tgctgtgata ggataaaagc aatatcttct ttttctgctc ctccttctga ggtaaagaat
     8521 tttattccat tataataata gggaagatgg ctagcagtca gcataaccgc agcatcgcac
     8581 gagaactgtt caaattgagt ggacataaac atagcaggcg tagtagctaa ttcgaaatcg
     8641 atgacatgta tcccttgttc tttcagactt tggatcaacg cctctttcag tgctggtccg
     8701 cttatcctac tgtcatgacc aatcccaatc gtccattttt tatttggatc ggttgatttc
     8761 ttctttttct ttatccagtt tgctagtccc cagccgatct tttttaccat gtctgtcgtc
     8821 aagtttactt catatccttc aactggtaat gcgattcctc gaatatccga tccattttgc
     8881 agcgctttca tatctttcat ataaaagtcc tcctcattta ttccctcatt ttttccagct
     8941 tttctgcaaa tcgctgtgct tcttcttctg agcggcaaat aataatgaat gtatctacac
     9001 cggccatcgt tccgacaact tcagggtaag ccacttcatc tagccaattc gttaccacat
     9061 cggcaccatt tcgttcagtc aatacgataa ccatgaactg aacagactgg atacgcaaaa
     9121 cctcgcgtct aattgctgta cgcaaacgtt cttcatttaa agaaacagat ggctggttaa
     9181 ataatgtata gcgtactatt ttgttttcat tttgactttt tactaacttc atttctcgaa
     9241 tgtctcgaga aatcgttgct tgagtcacat cggcgcctgc ttcatgcagc cgcgcaagca
     9301 gctcttcctg tgtcccaata ggttgttctg taatgatctg gcggatcagt gcttgtcgct
     9361 gtttcttttt catattagac acctctcaat gttgattata gcaaaagata agtaatattt
     9421 cttcatattt tggtattttt tacttttatt ttttgttttc attcaggttg ctaacctttt
     9481 tctattttaa cacgcaaaat cgtcttcatt ttttcaggtg ctgattttct tggatcgctg
     9541 atgtaaatct ctttgtgttc tttagaagct ctttcatagc cttcttttgc gatgaatgct
     9601 tctaatttct caaacgttgc tggttcttcg gcatagggcc cgacgtgtaa gatttgggca
     9661 caaagacctt cttccatctt tttgaatact acttgttctt tcaagtcttc cggaagtttg
     9721 ctagaacgct gaatggcttc ttgtgctatc gcttctgtaa caaagtcagg ctgtcggatc
     9781 atcaattgat aagaaaaatg ttcttttttc atcactggtt catgcaaata cttttcttgc
     9841 agtccccatt gtccttccag tggatagaca gtatacatct gataatcagg aatcagccaa
     9901 ttttttttag gcgacatgcg tattgcatag ctgactgcat atagacaggc tactcttcgt
     9961 ttaaagtctt cctgattcgg gtcgccgatc cctctgatgc taaagaatga ttttttagga
    10021 acctctagta tttgcgggct ttttttcata ctatagctct tgtctttacg ccattcaatt
    10081 ttttccattt ttcttcctcc gcgaaattcc atttagttat ttattatcat acctcttttt
    10141 gagtgacttg gctaatcgtg ttgacactat ttttcagttt gtgctagaat aagtgaagta
    10201 aataaatcgt acagataaca cgtttcggcg tgtcgagaga gaggcggttt ctatttttat
    10261 aatagaaatc actctctttt tttacgaaat tttttagagg tgatgtaaat gacaaaacaa
    10321 aaacagctgc ttttagtacc tgcagtaatt gcaacaggga tgatgtcttt tgcgggcgta
    10381 ttgatcgaga cggccatgaa tgtaactttc ccaactctta tcagacagtt tgatttaacg
    10441 actgcacaag tccaatgggt aacaacgatt tatcttttga tgatttcgat cattgttccg
    10501 ctgtctactt atttgaatcg caattttagt ctcaaaaaac tctttttagc ttcaaatctg
    10561 cttttcttgg caggtataat gatcgatttc ttctctccta ccttcatggt cctattgttt
    10621 ggtcgattgc tccaaggtgc agcgacaggt atcgctcttc cgttgatgtt ccatattatc
    10681 ttgacatttg cccccctcaa tcgacgaggc gcgatgatgg gtgtcggtac tttgacaacg
    10741 gccattgctc cagcaattgg ccccacttat ggaggcatta tgacttcaca ttttacatgg
    10801 aatcatatct tcctgtttct tgtgcccgtt ttgtttcttt ctttgattat cggctggttt
    10861 gctattcccg agatggcagt acaaaaaagt ggttatttgg attggataag cgttttagga
    10921 atttgtcttt tgtttagtgg aagtctaaca tttttgagta cgttgggaac agtttccagc
    10981 tggattgctt taatcatcgg cggtattggc tggttccttt tttactatcg aagtaaaaaa
    11041 gtggagcatc cactgatccg actgtctatt ctttcaaata aaacattccg cttgtttttg
    11101 attggcttct tagtttatca gtttttatta cttggtgtat cttttgtctt gccgaatttc
    11161 cttcagatcg ttcaaggaag tagtcctttt gtcgctggat tagcgatgct tcccggcgca
    11221 gcggttggtg ctttgctttc tcctttttcc gggaaaatgc tggatcaata tggtccgaag
    11281 aagccaattt ttgctgggct gattctttct ttgattggtt ggctagcatt attcctactc
    11341 ataggacacg cttcagtggg tgtattgatt gcttgtcatg tgttttatat gatcggtatc
    11401 ggattatctt acagcaatat gatgaccacc ggaatgaatg cactgaatga agaactacaa
    11461 ggagacggga acgcaatgtt caataccttg cagcagtttt ctggcgctgt ggctacttca
    11521 ttagtagcgg tcattatcaa ttacgtccag catcatactt cccataacta tgaagtgtct
    11581 actacattag gatcaaaagc agcattaggc gtcttgttgc tgttgcttct cgtaagcttt
    11641 gctcgattcg cctattatct gttctttgct aaaaaagcgt agattccata tacggcaggt
    11701 gttgcatctg ccgttttttt gtgtgaaaat ggttgtttgt aaaaggaggc ttttttatgg
    11761 aaaaaattgc cattatcggt ggcgggatca ttggtatgac cctagccagt tacttagata
    11821 catcaaaatt caatgttgct ttatttgatg aagggacagg acaggcaact aaagctagcg
    11881 ctgggattat ctccccttgg ttgtcgaaac gcagaaacaa acaatggtat cgtctggcta
    11941 aagacggcgc tgcttttttc cctaaactta taaaagattt ccaacttgat caaacgattt
    12001 acgcacaatc agggacactt ttgcttcgac ctgaaaaaga tttagctgaa ttggctgttc
    12061 ttgccgagaa acgaaaagaa gaagcgccag aaattggaaa catcgaatgg ctttcacctg
    12121 aacagacatc tcaagcactt ccgttattga aaccagcagt ttcactgcgt atcagtggtg
    12181 gtggtcgtct ggatggaaaa gcatatttag aacatcttgg tcagcgttta gcacaaaaac
    12241 aaatggagca gatccaggca aaagtcactt tgcataagaa gagcgattct tggctcatca
    12301 agggaccgtt tggagaatac acagctgatc gtgtaatttt gacacctggc ccggcactta
    12361 agcctttatt acaagaatta ggctatgctg cagatatacg tccgcaaaaa gggcagctct
    12421 tagcatttca gacatcttat aagatgagtc aagactggcc tgttgctatg cttgacggag
    12481 aagctgattt cattcctttc atggatggaa agatcctttt aggtgctacc catgaaaacg
    12541 atcaaggctg ggatttggct cctactagcg aagcttataa gggattgact aagagtacag
    12601 aacagttctt gactgatcca gatcagataa aaaactggcc atacgagttc cgtgtaggta
    12661 cccgtgctta tacgtcagat ttttccccat ttttcggcgt actgccagat gatccaagct
    12721 tactggctgc tagcggtcta ggctcttctg gcttgaccac aggtccttat atcggctatc
    12781 tattagccaa cttcttaaat actggtcatt ggcctacgga agattatcaa aaggacatct
    12841 ccacctatat tacgaaaaat ccatcataga aaaacagctt tttcaagtaa ggatcccgag
    12901 cgatattgcg catggattac ttcttgaaaa agctgttatt ttagttcaat ttctgaatca
    12961 aattctccaa actttctttt gcttcatctg gcaagagatc ttttcctatc tgagcttcaa
    13021 tgaatcgtaa acgaatatca agtcgttgtt tcaccagatt tttataggac gtatcctcaa
    13081 aagaaacagg aaaatctggt ataggtaaat agtggatgtc ttcaaatgga ccaaacgact
    13141 tgaattctgc cgtttgttgc acgattcttt caacgattga caatgtcgtt tcaggtttga
    13201 tttttttatc atcgaaatat ccagtattca ggacatagca ggctgtttgc tgtttcaaaa
    13261 acaactctcg aaaagcttga tagtcttccc taagaggata gacacggaaa ggattagcaa
    13321 atggttcaat gactagctca tctagctgtc tttttgtagt gatattttcc gcgtctgatc
    13381 gttttgttgc taatgttgca ccaaaagacg ctgctaaaac tgggtcattg atcttgataa
    13441 ttggcggaag cgaatcatct ttcatgatcc aaaaaacagc atcgatcttt tcgctaagat
    13501 gatcgactcg gtcgggtgtc gcataacgtg atttgactgt ccgcccattt ccgtttcgta
    13561 tatctcctgt tactaggact ttttctcctt tacttgtaca tgtcacgccc acattctgac
    13621 aagtaaggaa agaagaaatg gccggatcat cagacggata gtcttgtgtt ttatcaaagt
    13681 aagaaggttc caaggcagta gcaaaaccat tttttcgaga aataacgaaa gcatcgtcat
    13741 gcaagatcga aacagagtga ccttccgtat gttttgctaa ggtaatcgtt gattttccag
    13801 aaccggacaa cccaaataca gccatcgtat aggtctggtc attactagca tattgtttca
    13861 tcccaccgtg gcaggcaata aagccattgc gatttcccaa tgcccaagca agcgtcaaag
    13921 tcgctttttt taattctcca aagtaacgta atcctagcac agccgctgta ttatggagag
    13981 catcaaacaa aatgagtcca tttggaaatg tttcatgtgt ccattcggga tcacaaaaga
    14041 tataaagatc tccctcatca aaggaaacag acttttgata agtctctaag atcttcgaat
    14101 tcgctggctg aaaattcagc aaataagaat acaaattgtt ctcaaatcct tctggaatca
    14161 gcaaatgact ttgcacagaa aattcttcag ataggccaac gattactttt gtatggtaaa
    14221 actggcgctt gcgactttga tacacagctt ctcttaaaat tttctcgtat ttgctttgtt
    14281 ccttgtctaa gtcctcaaag atccgccgag cacttgctgt tcttcccaca atgcttccat
    14341 cattgaatac tagtgtccta acatcagctg gtaaatctag ttcttcagca tgtgagattg
    14401 ttaaatctgt gatgatcgtt cctggactcc cttttgctag ttgataagct gtcttcctat
    14461 caaaaacttc atgaacatta ttaccgtaaa atgtcgtttc tacaatcgat cgaatactgg
    14521 ataacagtgg atttccactt gtgatttcgt ttaattcgag actctctttt gtactcatgg
    14581 ctcctcctca ttccctaaac aaaaaaacgt tttctttatt atacattatt cgagttttta
    14641 tttgtactgc tgcgctgtgg gaagcaaacg agttattttt cacacaaaaa aaccgagaac
    14701 ttgaataaac aagtttctcg gtttcatcat tttcctttta tcggcttagt accagccgtt
    14761 tgctaaccag aatgttttag cagcatccca tgaaccataa cgagatgtta catattgttc
    14821 tgctacacgt tcttgattgg cagctgaata gtcgccgttt aagtatgaag catctaattg
    14881 ataacggccg atgtaacgtc cgttagtagc tgtgtatgaa ccgcctgatt ctttttgtgc
    14941 gatccattct tttgcagagc ttgaagttgg cgctgttgca gcagctgggg cagcaggagc
    15001 tacttcaact gtttcaacag gtgcaacgta tgtatgttct tgcgttgctg tttcagttgt
    15061 agatgctgta ttttctgttg ttttgttttc tgttgttgct gaagaatcat ttgaagttgg
    15121 gattgttaat tgttcaccaa cataaatgcg gttgatgttt gcgatcgaat ttttttcagc
    15181 aattgcatcg atcattgagt tgtctcctgc aaatttttgt gagatcttag ataatgaatc
    15241 acctgatttt actgtataaa cttcatcagc gtgagcattt gtagttccta caaacattgc
    15301 agtagcacca gcagcaaaag ttgtacctaa aacaattttc ttaaatgaat tcatattaaa
    15361 ctcctttttt tctccgtttt ttattctttc gacgctatta actttatcat ttagaaatat
    15421 ttcataggta aaagaaacat gacat
//
LOCUS       DANKBF010000065        15379 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000065 DANKBF010000000
VERSION     DANKBF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            54..857
                     /locus_tag="PWY89_002263"
     CDS             54..857
                     /locus_tag="PWY89_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2067271.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1442..1897
                     /locus_tag="PWY89_002264"
     CDS             1442..1897
                     /locus_tag="PWY89_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067272.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2016..3074
                     /locus_tag="PWY89_002265"
     CDS             2016..3074
                     /locus_tag="PWY89_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDL2067273.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3116..5065
                     /locus_tag="PWY89_002266"
     CDS             3116..5065
                     /locus_tag="PWY89_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2067274.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5068..8709
                     /locus_tag="PWY89_002267"
     CDS             5068..8709
                     /locus_tag="PWY89_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067275.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            9109..9447
                     /locus_tag="PWY89_002268"
     CDS             9109..9447
                     /locus_tag="PWY89_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067276.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            9557..9778
                     /locus_tag="PWY89_002269"
     CDS             9557..9778
                     /locus_tag="PWY89_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067277.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            9798..11954
                     /locus_tag="PWY89_002270"
     CDS             9798..11954
                     /locus_tag="PWY89_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067278.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            11966..12199
                     /locus_tag="PWY89_002271"
     CDS             11966..12199
                     /locus_tag="PWY89_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2067279.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            12189..13055
                     /locus_tag="PWY89_002272"
     CDS             12189..13055
                     /locus_tag="PWY89_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067280.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            13070..15040
                     /locus_tag="PWY89_002273"
     CDS             13070..15040
                     /locus_tag="PWY89_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067281.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            15207..>15379
                     /locus_tag="PWY89_002274"
     CDS             15207..>15379
                     /locus_tag="PWY89_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067282.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         5709 a         2175 c         3101 g         4394 t
ORIGIN      
        1 gtaccagatt aacatgatta aaggagcgca aaaagaagat tggagagtaa tcaatgtctc
       61 aagaagttac cgaaaagatt attgatagtg atgtagattt agtatcagaa caaacaaatg
      121 caattgtgcg ttataacaat ggtctgaata caacagcttt gcgaggattt aagcctgttg
      181 aaatggatgt gttttgggct attttatcaa aaatgaaacg tcaaggaaca gcagaagtaa
      241 cttttgattt caacctcttt cgttatttgg tcaaatacga cagaagagaa aaggacagtt
      301 tttattctgt gttaaaagat ttgagtgaca aactaggtaa tttaacctac cgttttgaag
      361 attcagacgt ctatgaacag ctgtggcttt ttcaaaaatt ccggatagat aaacagaagg
      421 aaacagttac aattaaggca aatgaggaat ttgaatttat cttaaactca attggcaaaa
      481 actttactcg ttttgaactt gataatttta caaaactaag aagtagctac acaaaagaat
      541 tgtatcgtca tctcatgcaa tacagaaata aagagtggcg aaatggttat tgggtagtca
      601 aagttgaaga ttttagaaga gcattaagta taccttcaag ttatagaatg acaaatattg
      661 ataaaagaat actagagcag gcgaaagaag aatttttagc tcctgatgaa aacggaatac
      721 aggtatttga aacttttgat tatgaaaaaa tctatgcaaa gaaaggaaga agagttgatc
      781 ggctcagatt cacttttagt gagccagaat caaacttgcc aaccatttca atgcataatt
      841 ggttagaaga agattgagaa gaagcaggtt gtaattttaa tacaacctgc ttctttaaaa
      901 aagtatgggt aacacttttg gacaataaat gcaagaagtt tttttgtaag caagtttcag
      961 tctaaataat atttgaaaga atggctaatc tatacaaatt ttaacaacta tgggtaacac
     1021 ttttggacaa gtcaagaaaa aaaacaattt ttcttaattt gttgatcgac taaaggtaac
     1081 acttttggac aacacagaat aaaaagtccg gtgatatcag ttttggtagg aaacatctta
     1141 atatctttgt gagattgaag gtaacatttt tggacaagtc aactatttgt taaaagttaa
     1201 tatagagtaa ttagataagt tttttctttt cgtctgataa atggataata ttagttattt
     1261 tattgctaat tagtctttca tgatgactga aatagctaaa ataaatatct cgttggataa
     1321 ttgctgatac tttaagggat atctaaattt gctgattgat ttatttatgt tattatgata
     1381 ttataataac ataaatgtgt tacgcccgaa cctcatttta aaaaaatgaa ggggtgagac
     1441 aatgaatgct attaattggc atgaatttgt taactatatg gagagcttac tagatgatct
     1501 aaaaagtttt agtattgctg atgcagtaaa gcatgggtta ccagttagta caacgatgaa
     1561 attacgagga ttgaaaattc cagatgaaat tattaagctg agaactaaaa caattattga
     1621 aaccgcaaaa gtcgttgaca atatgaacca tgaactaaaa ggcaagaaaa caacttacga
     1681 gaaagatttt gaacgttttt taaaatctgt tattggaaaa tatctaggta aaaaatcttt
     1741 cagtgaattg agagatgaag agattgtacc tactccaatg gttagcaaaa tccgaaaaaa
     1801 caaagccgag gggaattact atcttggtac aatagcaaaa atcattgatt atttggaaaa
     1861 taaagagcaa aagaaaggaa cgaagaaagg aagataattt ttgtgttaaa tattaatggg
     1921 gaagaatata ccgaaatgat tcaaaatccc gaacctagaa ttaccgaaaa aacgtctgat
     1981 tgtttgctct tttatgaaag ggggcagaca aactaatgga aaaaaacgtc aattcaacat
     2041 ctaccaaaga tcaatacagt attattggag ggttgcttgg tgataaggat aagagaacac
     2101 gtcacggatt acgcattatg cttgcaactt ttctaggagt gcttttttta ttaacaggct
     2161 tgggtagcta catttatcaa ttaggaaact ataattatta tctcgtgaac aatacaacac
     2221 ctataggctc ttcattggag tttgctcgat caggagcaga gatcactatt tcagatgtgt
     2281 ggacagatca aaacagagat gtaacagtag taaaactcgg ttactcgcaa aacgctagaa
     2341 agttgttatc tacgcaagga aaaaactaca acttatatat ggcaacgaaa gaaaaagatc
     2401 gtcctaaaaa gcttgaaatg agctatggga tgttaagtac tgatggggat gctttcttat
     2461 tcctaaaagg taaattagat gaaagagctt atcaagtttt tattgcaaac caagtatcac
     2521 taatttctgg tgatgataca gctgacgatg ataccaaagt caatgtgaat gaagcagatt
     2581 cagttacaaa agcactttct caatattcta tgaatgatgt taataatgaa ggagtaattt
     2641 cttttgggaa aaaagcaaaa cctgttaata ctcctgataa tatcaatttc agaataaatc
     2701 catattcaga ttcaactaat gtctatcaag gaagctttct taccaaagat ggcgatattg
     2761 attattcaaa agttgtagca gttacaagta agaataatgt tgtaagtaag ttagaagatc
     2821 agttaaatgc ttcaaatact aacatcaaga aaattgaagc tacaaaagct gagtatctgg
     2881 aacgtttaca acaaaatcct gacgataaag attcgcaaga aagtattcgt caactagaca
     2941 gtcgaatgga gaccgaaatt aaagcaatca atgatacaca aaaattgatt gatcaataca
     3001 aaaatgctga ctttaacaaa gattcttttg gagacatgaa gacgacattt aagtacctaa
     3061 ctctgactga ctagaagtaa attattctta ttttagagga agtgaacaca aacagatgga
     3121 aaatagtacc gagtttttta agaatattca aactgaattt aatcatgctc tcacaccaat
     3181 gaaaccaccg aaacataagt tagtgggacg tgatgacgaa ctccatgagt tgtcactgat
     3241 cttgaataga ccggaaacac cgcaagctat cttgttaggt gaagccggaa caggtaaaac
     3301 agcacttgca agtgaatggg ctgaaagaca gcgacaagat ggaaagaaag tacaagtata
     3361 caaattatca atcggacaat tgcgtgttgg gggagatgat gtcctccaaa cacgtttaga
     3421 aacattaatt ccaagattga aagaatatga gcgaatttta aaatctgagg acaaagaaaa
     3481 tgaatttgtc ctcttcattg acgagtttca taaagttgtt agctcttttg gtagtggcaa
     3541 taaaattggg ggtgattcgc taaaagaacc gttagcaaat gaatcacttc aattgattac
     3601 cgctactaca agaaaagaat atgaaaacta tatcaaaaga gatgatgcct tagggagacg
     3661 gttacaaccg ttagaattga atgaactaaa aagcgatgtt gtcgtcgata ttttgagaca
     3721 atggctaaat acatcctctg ttaaagatga tcctcaggct tcttttaaat tatcgcaaag
     3781 gattcctgat gaattacttc aagaaatcgt acaagcaaac aagctttatc gtgaggattt
     3841 gtccgaaccg gctaaatcaa ttgccgtaat ggaggatttg attgcaaaag atcgagtaga
     3901 cggaaatgct tataatcgag aaagtctatt gcaaatattt ggtaagtata atattcagct
     3961 agattctgat tttgatatgg aaaaacttca agaggaatta aagtccataa aagggcaacc
     4021 gttagcagtt tatactataa caatgttgtt taaacgtatg aagcttggat ttgatcgttt
     4081 taaaggtcga aagcaaccaa aagcaactgc tctgtttaca ggatcaacag gggtcggtaa
     4141 aactgcaatg gctaaagccg tggcaagagg actttatggc gatgaaaata aagttctctt
     4201 agttaatatg ccggactaca aaacaaaaga tgatgcaatg cgttttagac gactcatagg
     4261 tactcatgta aggcatgatc cttctgcgat tattgtgtgt gatgaatatg agaaagctgg
     4321 tgtagaaaca ctcgatgcct ttttatcaat tttagatgaa ggtaggataa gttttactga
     4381 aagaggatgg gatgatcgag aagttcacga cactgtttca ttgtcaaata caattttatt
     4441 tgcgacaaca aatgcaggtg cggagatgtt cgattcgtta aacagatata gctcactatc
     4501 tcgtgttaca aacaaggaaa attctaatga atatagcact gaactaaaaa gagaataccg
     4561 aaaagtcgca tcagggatca aacaagcctt gattggtgaa ggtctaaagc ctgaagttat
     4621 taaccgattt ggacgaatag ttccattttg ggctttatcc gaacaaactc ttctggaaat
     4681 tgcttataat gaaatgttga agagtgctga attagtacga aaacatttaa atatagattt
     4741 acgtcttcca cctaggaaag aaagagtata tggtcaaaat ttttatgcag acgatctctc
     4801 catgcactta gtttttgaca tggcttcaca agaggattct aaatcagggg gtgcaaggaa
     4861 tatcttgcat ttagttgaat ctgagtttga agaagcgatt actgaagcaa taattactta
     4921 tccaaacgca aaaatattcg atgttaagtt gagtgaagcc ggagcgtttg aaggaaaagg
     4981 tgattctaat gctgaaatta ttgcaaagcc ttccgttgct gaaagtaaag aaaacaagat
     5041 tgtattttcg attggagggg cgtagctatg aaattcaaga aatatttact acaaaacctt
     5101 agaataaaac gatgggtcgt aagaggtcta gttattggag gagtattagc agttacttta
     5161 ggcgctcctt ttggaatgaa tgatccaact cctgacaatc aagatagcaa acaagaaaca
     5221 gttataaata agaacgttgc atatgcggaa agtaaaccgg cagaacaagg aatgtggaca
     5281 ctatatgctc gtatcctaat gggagataaa gaagttaatg atagtgccgc taaaactgga
     5341 caaatgaatt tgttaggaca aggtggtatc agtgccgatt tcccttattc tgacatttct
     5401 aaacaaggta attcgattgc agggggtaat gcaggtactc aactagcatc tactttagca
     5461 acttatagtt actacggata tattgaaaca gtctcaggaa atgctattgc cagtcaagca
     5521 tcatccattc taggaagtat tggtaggacg attggtagtt ttatcggatc aattagttta
     5581 atcctttcta atattgctga atccataaat gaactccttg gaaaattttt catgagtatg
     5641 aatatatttt ctcttatggg gtggggagaa ggtggatcgg atgtatctaa tccggtatct
     5701 aaagcattaa ctagcttgct taacaatttg ggattaaata atgaattatt taaagcgata
     5761 gcaagcttat ctatgctctt tttcacagca atactaggtt atcaaggaat gaaattattg
     5821 tcgaaagatg gtgttaactc gaagggatta tcaccagtac ttgaaaaatg gattgttcgt
     5881 atttttgtat tgtttatatt cttaccagtg gcaggatcgc tattgggagt ttatatcaac
     5941 ggcatccagt cagcaaaaaa agatggagat ataccaacaa atgttgtttc tgactacatc
     6001 attaacacaa gactttgggc ttctactcaa aatcttgctc catcatctga tactggtggt
     6061 gatgtacctg acggaaagcc tacatccgaa atcaagttcg ttgatacaga ttatgaacca
     6121 tctaaaaaat ctgcaatgat aagagcgatc aatactagca gttatgccag acaaaataat
     6181 gatctagatt ctaattcagt cggtatggct ctattaaaag aatggtcatc gaatgatact
     6241 ttcaatatta atacttatgc cggtgatgtt aatgtcgccg aaaatgatgc ttatatggga
     6301 agagcgcctg ctgtttggac aggctataat ttaggggatg gaaaagaagg aagtgcaatt
     6361 aagaaaccaa ccaatttaaa tggatatatt tggtcagcaa atcaaaatgt gacagacgac
     6421 aatcgcaagc ctgatggaaa aggaacagcg cataactttg atgctactca accttggggc
     6481 gttgctaaca atgcaacttt tagcacacag tcggtagctt tattgctcca atcggcactt
     6541 gatacatcgg gagctaagtt ttatgcgtat aatatcgctc cttcaggaat tcaaggcagt
     6601 gctaagaata tgtcaacagt aaaaacagaa tggcgtacag catccttagt tggtcatgga
     6661 gctatgggta agtttggctc gtggctagcg atgaacgctg aatcaatcgt tttagcagtt
     6721 ctttacttag catgtgcttg ggcgatgttc aatgtgaata tcgtgaaagc atttaaacgt
     6781 gaatttattc ggatttggga agtagttcta acaggatcac ttgcatcagg agcaggtgtg
     6841 atggcaattt cattaggtgt tgttacaaca tctctgatcg gactacaatt acctatcttt
     6901 ctgattactt ttattcgtgc atttggtgat actgctgtgg gattcattcc taatacatta
     6961 gttggcgatg gcgtagcaaa tattgttcga tctgctatga ttttaggtgt aggatatctt
     7021 ttagtcttta aaaaaggcga aaaagtaaat aatttgatta atatgcttat tggcttacca
     7081 cttgaagtag cgtttgggtt tgatgaacgt gctaagttat tatttggtaa tagaaatgat
     7141 gctgtgcgag ttgtggacgg cgtttctaaa gcaatcaaga aaagccaagg agctaaaaat
     7201 ccctattatg atggtggcaa cagctcagta aaagatatgg ttgcaggagg tcttggagca
     7261 gttggagcgc aaaaaatggc tgaacgtttg aacaatatgg gagatagcaa tgctgatgct
     7321 aagtccattg ctgataaagt aattcctgaa acaccagaag gaatgtcatt caacggtggc
     7381 ggagttggtt ccacaatcaa taatacctct ttcaaagatt tagttcgtca agatcaacta
     7441 cgtaataact cccaacaata tattgatcat ttaggacgta tggttgatga aaacggaaac
     7501 tttgtcgatg aaaatggtga aatattaaaa gatagtaaat acggtaaaga tgctaaaggc
     7561 aactttattg acgaaaaagg gcgtttggtc gatgaagatg gaaaccttat agatactaat
     7621 ggtaaaccat tggaagatag tgaagatggt aatattggta tggatagcga aggacgttta
     7681 attgatgaaa aaggtcgctt agtcgatgaa gatggaaatc ttgttgacga aaatggacgc
     7741 aggttgctaa atggtgattt tggtcatgat agtaacggac gtttagttga cgaaaaaggt
     7801 cgtttagttg atgaagatgg aaatcttgtt gatgagtttg gtaataaact tggtaaacac
     7861 agcgatggat ataatgtgaa tggagattta gtcgatgagg atgggaatat cattgagacg
     7921 agagatgcgc ttgtggcaga tcgaaataga gaactcgaac aacaacggca tacgccaatt
     7981 gctgttgcaa ttcctgagaa tgaagaagac gagattactt tagatcaaac tcatcaagaa
     8041 ctgaggaatg aaatgagtgg tgaggacgtg tcagacagag tgcatcggga attaggagat
     8101 ggagcatctg gtgaaagtca gtcaagagag cttaacagca actttgataa tagtgctatt
     8161 cggaatgaca cagtacttaa agacagagaa tcgctaacaa aagaaacgga taaagaacaa
     8221 tcagatgtga agaaaaagtt cattcctact cctaatgtta atcaaacaga taaatatgaa
     8281 acaaatattg gatcaggaat gtcgaaagac aatcaatcaa ttagagaacc tgaactgaca
     8341 aataaaaata atagaacaaa taattctaaa tctacttcag ataattcagg gaaatctaat
     8401 caatcaacca agaaaacatc aacagctaaa tcagccgttg aatatctatc aaaaactggt
     8461 aaaaatgttt acacctcagc agataaagct ttagcaaaag ctccaaaagg tagtctagca
     8521 gggataacct atgaagctaa aaacgaagct attaaaatag gtaaaggaac ggctaaggtt
     8581 gccaaggcta ctgctacagg agcatccaaa actggtaaag tagtgaagaa atctaccatg
     8641 tcagtcaagg actatattgt aaaatacggt aatcaaaaat caactgaaaa taagtctgaa
     8701 gacgagtaaa aagaaggagc attgatttgc tccttctttt tactttgatt tctaactgaa
     8761 gatgtcaagg aaaatttcta tttatgcaaa aaaattattt taaattgtct ataaatgaat
     8821 taaatattta ctgttcaaag ttgtataatt aaaaagggct tatgctggta aagaacagaa
     8881 ttattatcta aacaaaaact tgataaacca gcatttttag ccaataaaaa aagtgaaata
     8941 atttaatgga aaatctcttt taatcatcga aaatttatgt tattatgata acataaagat
     9001 gtaaatatcg agacgtcttt ttcttagtta gatcattgac aagtgaacaa ataagatttg
     9061 tcgatgatat aattaactta ttaaaaatat taaaaaggag gtttttatat gttcgattct
     9121 attaaaaatt tggttagcgg aatgacgcct gatatgaaaa ctttagtaca agttgttttg
     9181 tatttcttgg cattcgtttt ttcgttgaaa ccaggattca ctgcaatgaa attttttggt
     9241 gatgcaaaat ggggagatgg attaaaatat gttggggcga ttattgcaat ctttattatt
     9301 cctgtaatta caaccgttat gctttacaca atgggtacta aaacaggtaa tgatttgaac
     9361 caaaaagcta atatcatatc agcacttatt ccagttgttg gtatctatct agtttcaaaa
     9421 tttccaaaaa atgaactgtt aggttaaaag tgaattgaac aaatttggat aatatgccga
     9481 tttgttcaat ttacttttta ccaaaaaact acgctccaag cgtagtttag aagtgaggga
     9541 agaaagtaaa tgtaagatgg tttttgatag agaaaaaatg ttagctcacg ccaacgaagt
     9601 attaatgagc agtctgaaag gtaccgaact ggcaaagata atgaatatga atgttaacca
     9661 gttctatgat tatcgaaatg ggagtaaaaa gattgaaaaa gccagacttg aaacgcttat
     9721 taaattcgaa aaagcatatg tatatatgct agataaacag aaaagaacta ttgactgaaa
     9781 aaaggagttt tacaataatg gcaaaattca aagatattac cgaatctatt aattatgtgg
     9841 cagaaacagt tcctgtagtt gcactctctt tgaagccaca attactcgaa gtgtacaaaa
     9901 aagaagaaaa agaagcgaag gcaaaaaaag ctaaagcaaa ggtatggaaa gaattcgatg
     9961 cgttgctgac caaagattta ccaaagaaaa attatatggt aattgaagat gtacaaaagc
    10021 tcaacagtga gggagttata gatgaggatg atgctattct cgattctgag aagtatgtcg
    10081 ttctattgaa gtcaagtttg aattatgtag ctgatctacc agaaaaattt aaagagaagc
    10141 tattagcagg tgtccgtttt atggatagta gtggtcaatt attagttatt ccggatgatc
    10201 ctaatgtgtt tgaatcagaa gttgaagaag ctacagactt ctatagcgca gtagagcaat
    10261 tagaagtagc tactaatgga ttagaacgaa ttgctgagac cttcaactct tctgaagaag
    10321 ttgaatttga agccgtagca gaaaatgtac cagatgaaaa agaagctctt tttgtggatg
    10381 ataacaaacc aaccgaagca gaaaaccaag aagtaagcat agaagaaaca caaatacaaa
    10441 cagaaaccta ccaacaacca aaaattgatg aaaaacccga atcccaaagt gacaaagaaa
    10501 attatgttta tgaagctgaa aaaatcacat ctgaaagtga tgtaccgaaa aacttagatg
    10561 ctgatttttt agaaaaaaat tttgaacaga tcattgctga caaatttcaa cctgtagttt
    10621 tagatgagat taatgaccct gtcgatttaa acgaggagat gctatccata cctgagttga
    10681 aaccattgct gtttttagct aaagagagta tgaacgctcg catctctatt gctaataata
    10741 aaatcaacag tgaattaaat aagacgaagg agcaattgat cctcaaagga aagcaaatgt
    10801 tagctgatga gcttttgcgg atagaggaag caacctcatt aaatggtcaa aatcagttta
    10861 caaatacact atcggttgct aaagagagct acgaagcaga agcatctaaa attgatgatt
    10921 tagtgatgga aaaatccaat atcattcgta aagattatga tatggcgaaa caggcttatt
    10981 tggatgctga actaaagaaa ctagagcaac ggtatgatgc tgagcattta ccagaagtta
    11041 aacaacaaga agatgaatat agaaaagcaa ctgaagaaag tcttgatcaa cagtaccaag
    11101 aagcaattga atctattaaa aacgatgcat tgaaacaaag ccaagaagca aaagaagttg
    11161 tggtggaacg aattatcaat tcattgaaac cagaaattga aaaatcatta acagacgtgc
    11221 aagaaaaaac tttagagatt gtcgaaaaaa caaagtcaga aaatgaagct gagttccatg
    11281 atacaaaaga gaaaacaaca gatgtggttg tcaaatattt tgcgaacaaa gaagaagttg
    11341 aaaagcaggt agctaacaac acgaaagaat tagaaacaaa acttgtagag caacaaaaaa
    11401 ctatcgaaga atataaagat cgaatgaaag aggtcaaaga tttagcagag ggttataaat
    11461 taaatagcga aaagttggca gaacaaagtg aagctttaag agaagactta tctcgttccc
    11521 gaagtgatct agctcaaatg gcagaaaaaa cgaaatcatt agcagttgct acagaaaatc
    11581 aatcaagttc aaaacgtgat tttacaagta aaaagatggc tactatcatt tctgtcgctg
    11641 caatggcttg cggaacgatc ttgggatcgt cctactttat tagtcataat aatcaacaag
    11701 cacaggctca aactcaaaca tctatttcga gagtggaaaa ttcgattggg acaaatacga
    11761 actctgcttc aacaaatagt agctatgaag caacaccaac tacagagttc aaagttggga
    11821 aagatgttgt catcagtatt gatgggaaaa caactaaggc tgaagtgatt tctattgatg
    11881 gagaaaaagc agttgtgaaa actccagacg gaaatcagta caaaattcaa gctcacttat
    11941 ctgataataa ctaaggaggg ctattatgcg agaagaaaaa atagtttatc cagtaattca
    12001 aaacaatcaa gaaaacatga cctcttctca gcacttacag gaagcacagc tcgtgtatca
    12061 ggtaaatgaa ctaccacaaa cgaacgagac aatgagcaag tcaatatctt taattggctt
    12121 gcttattgtt gcaatagtga taactgtagg tgtttaccgt aagttgaacg tgaaaggaag
    12181 tatttctgat ggcagataac tcaaactatg gatttaaaca taacttacat caaaaacctt
    12241 ctgaaacaat tgtcagagtt gaatggcttg aaaagcatgt caatagcacc tattctgtta
    12301 cagcaaaaag attaattgta tcagctatag ttgttgcagt cgttgttctt ggatcaattg
    12361 tttacttagt ttctgatacg attaaactga aatttatttt tgttccaacg ttattaatga
    12421 taattggatt tcttattcta attagccagt atatgtttat tgatccatct agtggttcat
    12481 tgatttctgg ttctattaga ctgttcaaat cagtagttaa ttcagcaaga gtatctatgg
    12541 ggaaaaagaa accatttcga ggcacagaga ttttaaaagt gaaacaagat ggaacaatcg
    12601 tatatcgaga tgggagtgta gcattaatgt tcaatctaga tggggctaca tcactaaccg
    12661 cttttccaga agaagttatg gctcaggaat cgaaatcaat acaatatcaa aataccagag
    12721 atcgttcctt agctgaaatc aaaatcactt catctcaacc tcagaatacg gaaagacaaa
    12781 ctcgaaatat gaaaatacaa aaacgaaatg caaggagtaa caaagcagtc tttgacataa
    12841 tagatcagca agaacgattt gtatcagaat atattgatgg tgtgaaaaca acagtaatcc
    12901 aatatcttat cttgaaatct caatctaaga tggtattgga tgaaggtgtg gaacgtttag
    12961 aaaactatac gagtagagga ctttattata gtgtcactca gttgtcagct aaacaaacaa
    13021 aagaaatatt atctgatatt tatgcactga aatagaggtg agagattaaa tggaaattat
    13081 gggtagatca caactattaa atttcaaaaa aaagagaaaa actaaaaaag aacaaaaaac
    13141 tagaaattca aaggtagtga ataatacctt tgttagtgat ttaacatctt tagttggtta
    13201 tcaatttaca ccatcttaca ttaggacagg taagcggtgg ggaacaattg tgaaattagt
    13261 gaatacttat ggaatgaata gaaattatgg atttggttgg atggttaaag ctattcctga
    13321 agtgagagta gaaggggtaa aagcctatct aattgaagca gataagccag tgtcacaaaa
    13381 agatcaacaa gaaataatgc acgagaatgt tgggggatca acgaatagtt atgaaaataa
    13441 tttagaagga aaaaattcag atgctgaaat tagcaaaact gttatgcgga ttatggattt
    13501 gaatacagca tctttactag acagcatggc aaataaaatt attgattgga agctagaaat
    13561 cctattagta tccgattctc ctaataaaat cagcgaacag ctaattaaac tccaacggtt
    13621 gtatgatgaa aaaatgtatg gattaaagct tatgtcatat ggaggagatc aacgtgatct
    13681 gtttgataga cttttaagtg aaccaatcgg gaatgtctat gagtatacgt ctatgagttc
    13741 ggattttgca ggaaatgatc atgcaattcg caaaggttta gatgatgcga atggatgggc
    13801 tatcggcact atgtctacta gctacgcaag tggtgtagct tttatggcac tggatgattc
    13861 ttttacaagt aaagttttag tggcatcaca caaaaacgct acaattaaag gctatgatga
    13921 aaaaatctca gcatcatcac tttgggggca attgattgct aataatgcaa tggcaaatca
    13981 taaacgaact tttcatattg tcttaaataa atttgattac tttggtgatc caactggtag
    14041 aacatttgtt gccaaacctt cgttaaacag tgtaatagaa aaggttaatt tggctgaaga
    14101 aggtcttaac tctttggagc tttttggtaa tcaaaaggac attggtgatt tatatagacg
    14161 gaatctcgat aaactaactc agatttttta tttgttagca ggcagaacat tagattctgc
    14221 aactagagtt ttattgaaaa aagaattagg taacttttat ataagtcaga aactttggga
    14281 tattaattat gaagaacgtt caaaactttt ggtgaacagt agtgatacaa tgccagttct
    14341 tggggatttt attagctatt tgactaatgc tgaaacaaaa gccaaaaaat ctggtactga
    14401 aaaagaagtc gatcgtatta ttgtattaaa agacacattg agaacagcat tagacagctt
    14461 ctctaggatg tttaatcaaa gaacgacact gaaagaaatc ggtaaaagtg acaaattaca
    14521 gttttactat aacttaggtg atctaagagc agaaaccaat gttatggaag ctcaattttt
    14581 aaataccttt gatttcatca catcacaagc aaaaaaagat gatattgtta tgattcacgg
    14641 cttcgataag ttatctattg aaaccgttgg gctaattaaa aaaagattaa caatgcttga
    14701 agaagatgga attcgtcttg cctttctatt tgatacaatc ggttcaggag aagacaatga
    14761 atacaaagaa gatattgaaa cagctgatat attcaataca gatggaaaaa tttaccaaag
    14821 ttttgaaagt gattttaatt acactatcat tggaaagatg accagtggag agttaatgaa
    14881 gtatgaaaaa aaagttgctc ctgctagaaa aggagaagat gatcaaaaac tttctgttga
    14941 tttgagacag tatttgacaa caggaagaag taataaattt cagatcagga gaccagtgga
    15001 tctgactaat aattttgtta ttggagagtt tattctttaa gttttatctt tgattttgag
    15061 tgtataagga aaggataaga aaaaacatgt atatttgaga ctaaaaacag tcttagagaa
    15121 catggtacgt tttgtgaaaa gagaactata gaaatttcat aaagtgaaac cttactgagt
    15181 gaaataatta tagtggaggt taaataatga aaccaaacat aaaaatttta gatcgaatat
    15241 ttctaggtag agacacagaa gtaatcctaa ttcaacatga agaaggattt gaagttagta
    15301 taggtattca gaaacttcaa aaacctcatt attgtaatca gttgtataaa aactttactg
    15361 atgaagaaaa agcgagagt
//
LOCUS       DANKBF010000066        15314 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000066 DANKBF010000000
VERSION     DANKBF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            278..787
                     /locus_tag="PWY89_002275"
     CDS             278..787
                     /locus_tag="PWY89_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2067283.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="PWY89_002276"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="PWY89_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2067284.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="PWY89_002277"
     CDS             2322..3302
                     /locus_tag="PWY89_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2067285.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="PWY89_002278"
     CDS             3424..4842
                     /locus_tag="PWY89_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL2067286.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="PWY89_002279"
     CDS             4913..6382
                     /locus_tag="PWY89_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2067287.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="PWY89_002280"
     CDS             6392..6859
                     /locus_tag="PWY89_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2067288.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="PWY89_002281"
     CDS             6895..7767
                     /locus_tag="PWY89_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2067289.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="PWY89_002282"
     CDS             7849..10383
                     /locus_tag="PWY89_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2067290.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="PWY89_002283"
     CDS             10539..11312
                     /locus_tag="PWY89_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2067291.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="PWY89_002284"
     CDS             11305..12096
                     /locus_tag="PWY89_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2067292.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="PWY89_002285"
     CDS             12106..12597
                     /locus_tag="PWY89_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2067293.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="PWY89_002286"
     CDS             12615..13025
                     /locus_tag="PWY89_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2067294.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="PWY89_002287"
     CDS             13029..14429
                     /locus_tag="PWY89_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL2067295.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="PWY89_002288"
     CDS             14452..14826
                     /locus_tag="PWY89_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2067296.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4783 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttct
//
LOCUS       DANKBF010000067        15255 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000067 DANKBF010000000
VERSION     DANKBF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            248..1021
                     /locus_tag="PWY89_002289"
     CDS             248..1021
                     /locus_tag="PWY89_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2067297.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="PWY89_002290"
     CDS             1018..1785
                     /locus_tag="PWY89_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2067298.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="PWY89_002291"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="PWY89_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL2067299.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="PWY89_002292"
     CDS             3750..4070
                     /locus_tag="PWY89_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL2067300.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="PWY89_002293"
     CDS             4070..4420
                     /locus_tag="PWY89_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2067301.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="PWY89_002294"
     CDS             4587..5525
                     /locus_tag="PWY89_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2067302.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="PWY89_002295"
     CDS             5540..6370
                     /locus_tag="PWY89_002295"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2067303.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="PWY89_002296"
     CDS             6414..7439
                     /locus_tag="PWY89_002296"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2067304.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="PWY89_002297"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="PWY89_002297"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2067305.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="PWY89_002298"
     CDS             8517..8957
                     /locus_tag="PWY89_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL2067306.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="PWY89_002299"
     CDS             8960..9562
                     /locus_tag="PWY89_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2067307.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="PWY89_002300"
     CDS             complement(9625..10728)
                     /locus_tag="PWY89_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2067308.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="PWY89_002301"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="PWY89_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL2067309.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="PWY89_002302"
     CDS             12294..12689
                     /locus_tag="PWY89_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067310.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="PWY89_002303"
     CDS             12689..12940
                     /locus_tag="PWY89_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067311.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="PWY89_002304"
     CDS             12983..13378
                     /locus_tag="PWY89_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067312.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="PWY89_002305"
     CDS             13375..14631
                     /locus_tag="PWY89_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HDL2067313.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="PWY89_002306"
     CDS             14643..14966
                     /locus_tag="PWY89_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067314.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="PWY89_002307"
     CDS             14959..15225
                     /locus_tag="PWY89_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067315.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DANKBF010000068        15221 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000068 DANKBF010000000
VERSION     DANKBF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            102..902
                     /locus_tag="PWY89_002308"
     CDS             102..902
                     /locus_tag="PWY89_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067316.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            990..1796
                     /locus_tag="PWY89_002309"
     CDS             990..1796
                     /locus_tag="PWY89_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067317.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            2028..2897
                     /locus_tag="PWY89_002310"
     CDS             2028..2897
                     /locus_tag="PWY89_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2067318.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            2917..3264
                     /locus_tag="PWY89_002311"
     CDS             2917..3264
                     /locus_tag="PWY89_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067319.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            3277..4539
                     /locus_tag="PWY89_002312"
     CDS             3277..4539
                     /locus_tag="PWY89_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067320.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            4619..5995
                     /locus_tag="PWY89_002313"
     CDS             4619..5995
                     /locus_tag="PWY89_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2067321.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            5992..6621
                     /locus_tag="PWY89_002314"
     CDS             5992..6621
                     /locus_tag="PWY89_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067322.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            6633..7103
                     /locus_tag="PWY89_002315"
     CDS             6633..7103
                     /locus_tag="PWY89_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067323.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            7100..7582
                     /locus_tag="PWY89_002316"
     CDS             7100..7582
                     /locus_tag="PWY89_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067324.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            7579..8241
                     /locus_tag="PWY89_002317"
     CDS             7579..8241
                     /locus_tag="PWY89_002317"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2067325.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            8228..8596
                     /locus_tag="PWY89_002318"
     CDS             8228..8596
                     /locus_tag="PWY89_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HDL2067326.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            8677..9129
                     /locus_tag="PWY89_002319"
     CDS             8677..9129
                     /locus_tag="PWY89_002319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067327.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRIRITEQAIEKQKDKEKKEIMSIYLSSGASFFHE
                     IDSMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            9781..9834
                     /locus_tag="PWY89_002320"
                     /pseudo
     CDS             9781..9834
                     /locus_tag="PWY89_002320"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9838..10437
                     /locus_tag="PWY89_002321"
     CDS             9838..10437
                     /locus_tag="PWY89_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2067328.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNISLIKQKN"
     gene            10453..10929
                     /locus_tag="PWY89_002322"
     CDS             10453..10929
                     /locus_tag="PWY89_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067329.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            10946..11848
                     /locus_tag="PWY89_002323"
     CDS             10946..11848
                     /locus_tag="PWY89_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067330.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            11921..12112
                     /locus_tag="PWY89_002324"
     CDS             11921..12112
                     /locus_tag="PWY89_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067331.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            12276..12863
                     /locus_tag="PWY89_002325"
     CDS             12276..12863
                     /locus_tag="PWY89_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067332.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            12863..13762
                     /locus_tag="PWY89_002326"
     CDS             12863..13762
                     /locus_tag="PWY89_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL2067333.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            13762..14676
                     /locus_tag="PWY89_002327"
     CDS             13762..14676
                     /locus_tag="PWY89_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067334.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            14728..14943
                     /locus_tag="PWY89_002328"
     CDS             14728..14943
                     /locus_tag="PWY89_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067335.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         5838 a         1966 c         3113 g         4304 t
ORIGIN      
        1 aataatgtat ggaaaagcta taaatcaata gataagatgt agaaaatctt atttgtgaat
       61 gaaaaacaag tcatcgtaaa atattaatag gaggtgtagt aatggtggga atatcaaaaa
      121 cagctttaga ctcattagct aaactattta gtagcaggtt tgatggtgca gaatttgttg
      181 aatatctaag ccaaaacgaa agtggaaaaa ctctgatcga aacaatgaaa aaagactatt
      241 tgaatgaaaa tgactttccg acatggacga atattgaaga tgctacatgg gatgtagtat
      301 gtacgatata ttctccttgg gtaacgattg ctgattttgt tttagataaa attggagggg
      361 atacataccc tgatgccaaa tctcttgcta caagtgactt taaaacgttc tatggttatc
      421 taataggtct aataggcaat ggaatagatt tgaaagttac gccaaaacaa acacctgaag
      481 aaattgaaaa agaaaaagga gaggttcaaa tggcttatgc aaatgacaga gaaaaagtaa
      541 aattattgga aggtaaatta tctgaagaag aacttggaaa aatggtgcaa gaaatcaatg
      601 aagacagcaa ctattttcct gaattgaagg tatatcaaaa tgacgaagat ttctttaatg
      661 aaatgtttag taatagtcca tgggaagcag tgagtaaaac aaatttagga gaatggaact
      721 ttcaagatga tctagttgca tttagcaatg atgggtattt acgatcatat tcagaaagtg
      781 agtatagagc tgaattaaaa acgtatgaaa cggatattct gatgagctat ttaaatctat
      841 tagagagtgg tcagggagag ttaagagata aagttgaaga actattaaaa gaagagctat
      901 agggattatt tcaacataaa cacaagcaga aaactactaa acaaaacgta ccatgcaaaa
      961 agtcggttaa accggctttt tgctctatta tgagaggaga tcaaaccatg agtgcaacaa
     1021 ccgatatggc tttagaagca gtaataaaag ataatacagg aaatgtcgaa aacgtagaag
     1081 actttagcga tattgcaatc aatctttcag catggatttc agctgtagga gaagaaaata
     1141 tcgaatttga gatcaagaat atggatgact taaaagcaaa atttcctttt atcgcatcga
     1201 aactaagtca agttgccgag gataaatcgt ggaatgaatt agttagtttt tccgaaaaaa
     1261 tttatgatat tacgcaagaa gatgagtgtg tttcatggga aatgaataat caatacaaga
     1321 aaattgacgc tttcgtcaaa tcacgtatag aaggaacagc ttttgagttt gaaggtatag
     1381 ataccgcaga taattggtta aataaagaat cctttattaa aagtatagcg gatgatcttg
     1441 aaaaacaatg gtctaaggaa gactatgcag agtacctaaa aacagaaaga gatacagagg
     1501 tgaaagaaaa actcactgag gtatgtttgg atgaacatat tgatcccaat atcgtaaaac
     1561 aagaagacta tgaagtggtt attgataaag ttcctactga ttttgaggaa gttgtaaagt
     1621 atctagttga agaagcatta tcgcaatact cttcagatga ggaagtggac gaagaaactg
     1681 ttcgtaccag agtttggcaa ctggtttttg atgacaaaga ttataaatat ttgagtgatc
     1741 aaaactcggt taattggagt gtacgattat taattgatca aaatgatatt gaataaaata
     1801 gagctgaaaa gctctatttt ttttgttaaa aaaactagct attttattaa aaataatttg
     1861 gttcaaattt tattttttta acttgataat ttcttgccat ccttttttaa attggctata
     1921 tatcaaggtt tatagtggga ataaataaaa atacatttga caaaatttac aatatagttt
     1981 attatatatt tatcttatta tgataacaca ataaaaggaa tggtggtatg aaaaagaaga
     2041 agtttagtcg atcgttgtta ggaattttgg ttttagttgc ggttggtctt agtggcaagg
     2101 gttactttca gaataacagc actcaggaac atgattttca gatcaaaagt agtgttcaga
     2161 taggtaataa agcagacagt gaatcaaaag aatttttagg gatacgttat gatccaaaca
     2221 aatatccaaa taatatcgta aacattaatg atgggaaata tacactgaca aaagaagacc
     2281 atgaagcatt atcagaaaaa ggcagtcaaa ccttttgggt aaattatgca gatttagatc
     2341 aacttggaag agcaggagat gttgaagcgt tgatcaccta tgatgctgta aattctcact
     2401 catctaaaaa tgtggaacgt ccatctttca aaagttctgt ccatattgca ggagaataca
     2461 aggatggagt atttgatcaa gagagagata cttgggtcgg caaagattca aacaatgaaa
     2521 tcactcagct agaaggctat aaaggttatc tctataacaa aagtcattca ttagcatggt
     2581 cgctaggagg agatatggag gtacacaatt taacacttgg aacgagagcg caaaatgttg
     2641 ggaaaaatga tcagaatgga gggatggctt actcagaaac gattgtacgt gatgcaatct
     2701 ataaaaataa gaatgaaaaa gtattgtata aagttgatcc aatttataaa gggaatgaat
     2761 tagttcctcg tggttctcat gtcgaagctt tctcagttaa cgataatgga aaaacggtaa
     2821 accttaatgt ttatgtattt aacaatcaaa aaggaatcaa catcaactat caaaatggta
     2881 cttgggtaag agattagaaa ttgagaaggt gaagaattgg aaacaataag catcaatgga
     2941 ttagataaat cagttgcaaa tcgaataaaa aaaggaaatg gctactatct tctgaagaag
     3001 ggtaaatatt cagttgctag agagttagaa acaatgagta tcgtgtatgt agctaagaat
     3061 cattttgacg tagaacaggc tatttttgat ccagattctg aaatagggaa atgtgttgaa
     3121 ttatcagaga aaactgttag tacaaaaggg gcgatggtca ttgagagaga aatgttagac
     3181 gaaatcagag taatcattcc taaagatacg cagatgtgtg aggacagcat aagtgacgat
     3241 gaaccaaaat cagaaagaaa ataaggagat attcagatga gcgaaaaatc cattgaatta
     3301 aaggaatata agcgaagcga tggagaaaaa tattatcctg tagccagttt ttctaaatca
     3361 gtatatctgg ttggacgtgc attggtagtt ggtgcaattg gcatatatat tttgcattgg
     3421 gtaacaaagg ttgtacaact gcttgtagat accttagtaa gtattcttgt ctcgttaaat
     3481 gatgttaacg gaacagatga tttaggatta aaagagatgt ttaaagagtt actcaatgcg
     3541 ttttttccca attatttaag ttattcatat aatgtacaaa gcttttttgc aaagctacac
     3601 ttggtagaca tctctatttt agtattagaa gcaggaacgg tattctttgt tggatatttg
     3661 gttactttga tgttcaggca tcactatgga gaacaaagac cattttttga tgatatggaa
     3721 agtagaaggt tgaaaagaga agctctagaa gctatggatg tgaagtatga tccaaacaag
     3781 attataggag atgaaacgaa caacaaaaaa agaaaacgaa acaaaacgca attagaatcc
     3841 gatgtgctat caacgcttaa aaatatcaaa atttctgttc atacaagacg tgatctaagt
     3901 gtcaatgaag atattcgaga atatatcgta aaaatcccgc aaccaaaagg gaagaaagtt
     3961 agagaaacag ttttacgaca aattcaagat ttagggtcga tcctgacaag agcaacaagc
     4021 ggacgagcca aatttggtga aaaagaagat gaagtttcag gcgattattt cattttccga
     4081 gcatcagtaa atattgaaag agaacgtaag gaaagagtaa gtaccaaaaa caaagaaacg
     4141 gaaagagcaa catcttcaat cgcagtttca aattcacaat tttcttttcc aatcaatctc
     4201 tttgccgata attccaaaaa tatccaaaac caaaccgaaa aagctagaaa atatgccgaa
     4261 actcaaaagc aatctttaga actttatcta tcttctgctg acgtacctgc aagtctaaca
     4321 gatatgcacg ttgggaatac atctattgaa tatttgttta ctttatctcg taccagaaaa
     4381 attcaactaa gctcaatgaa agaagaaatt gaaacttact tgcatatatc aggtgtaatc
     4441 gtgcggatca acgcagggcg tattcaaatt actttaccat taggagaaaa tgatcgtatt
     4501 ccaattgatg taccatcaat gatcaatagc gtgttttaga agacatcaca aatagaaaca
     4561 gaaaatacaa actaccagtt cacaaacaga aaagtaccga gaaaagtgag gaatagaaat
     4621 ggctatgaaa ttattagaga aagtcaaaaa gagagccgaa aaagctgaat ctattttgct
     4681 agataaagaa cctaatataa agcttaatgg aaaattcagt gtcaatatgt tgaacacaaa
     4741 ggctaaagac ccaacagatg ctatttttgg aattaagcct gatggcacaa ttgctcattt
     4801 ttgcatcagc gaaatgccac acatgttggt tgcaggaacg acaggatcag ggaaatcagt
     4861 attactgaat gaaattcttg ttacagtaat gtgtcactca acgcctgatg aattaaaaat
     4921 aggaattgta gacccaaaaa gggttgaatt tggaagatac aaaaaattgc cgttcatgtt
     4981 ggctgatcca attacagata tggatgaagc ttatgatttt tttgaatatc ttgtgatctt
     5041 gatgcatgaa cgttacaaac tgatggaaaa agcagggtta caaaacatca cgctatataa
     5101 cgaatatgcc gaaaaaaatg gattagatag atttccctat gtgatcttat tagttgatga
     5161 atattcacag ttggttggaa cacataaaga agtagaaggt ttgattgttc aattaggaca
     5221 aatggcaagg gcagcaggta ttcatgttat cttagcaaca caatctcctc gatcaacagt
     5281 tgtcactggt attatcaaag ccaattttcc gtcacgtgtt tgccttatgg tagcaagtga
     5341 tttagaaagt aggattgtat tagatgaagg cggtggagaa agtctaagtc caaagggaga
     5401 tatgcttatt aagcttgttg atggaagtat ggttagagcg cagggagctt acatcagtaa
     5461 caaagaaatt gaaacaatct tcaatcacct tagaaatact atgccagaac cagaatatgt
     5521 tgattaccga gcaatcgtag ctgaaactcg tggtgagggc ggtgaagacg aggattttgg
     5581 tggaattggt aaagctcctg taagaaaatc tgctaaaaga aatttgaaca cacctttagc
     5641 aaatccatca tctaaagagc catcaattga aaagaagagt gcgtttgcta ttaagcttga
     5701 aaaaaataaa gaagaaaaag ctaatcaggg agctaagtca ctagaaaaac ttcagcaaga
     5761 aaaattagaa aaactagaaa aagcattatc atcatcaact tctgaaactc aatcttcaaa
     5821 gggtatagat aatggacaat caattacaac taaaaacaag cctaaaaata gtaattcaaa
     5881 tctctcggta aatgttgtta tcacaactga taaatctgaa aaaaaacaag tgaacggaac
     5941 tacttcaccc atcatgcaaa aaatgataaa ggcaaaaaaa caggtgcaga aatgattatc
     6001 attagttcct ttttgagatg gctaacacca tataaatata cagatgaaat agttgagaag
     6061 cgagcgaaga aaaaagttaa aaaaacgatg ttaactccta aagaaatgct agatcgtgga
     6121 atattgggag tgaaggtttt agaagtaaac caaccgtttg aattggataa tgcgagagat
     6181 aggttgctta gagctgaagt tgatgtcgca tacttttatc atgataaaag caagcatcaa
     6241 atagctatca aattcaatcc aaaatcggca tggcgatacc aagcaagaga tgacctaaag
     6301 cgtaggaagc tgaaaccgat gctctatcct gaaggaaaag aggcatttga tagaacccat
     6361 attgttccca ttggatttca tggtacagag tcagataacc gcctattggt agggtggaac
     6421 agctcacaaa atagagttca gatcaataaa tttgagaaga aggtaagaaa atttaacgat
     6481 cggaaaataa tactttggtt tgccaatatt cagaagcaac cagatgggac agcaaagtgg
     6541 gaagcggtca tatttgatga agatggcaaa aaagtaatgt ctgaagtatg gcatgataaa
     6601 gctaaatttg tttggcagta gaggtgaaaa ttgtgttaac taacctaaaa ctatatagag
     6661 agggatttaa cgaaaaagta agtatcggtg aatctattga atatttagga aagaagtaca
     6721 ttattattcg tattcttgaa attaaaaaac tttattttag taaatataat aatttagcag
     6781 ttagagttct cgtccaaaag gttggaaatc caagtgacta tagaagtgat taccataact
     6841 ataaaacaga atcatctgta attaagcgtt ataatcaatc aaaaaatgat gagagaatat
     6901 tcaaagtagg agaaattatt atttctaagt atgggatagc atacgaaata gtttctatta
     6961 aaaaatttta ttatgaattt gttgatttgg ttgtagaata ttctgttcga ttgatcgttc
     7021 cgtggagtaa agaagaaatt aatgaggcat taaaacatga acgaaaaagt acattcaaag
     7081 ttttagaagg tgaaaaaaag tgaaaacaga ccttactagg caagctgaga agtgcttatg
     7141 gcatgaaaca aatcgcatgg gtgtatttgg ttgtttcgaa gtcactatcg gttgggatgg
     7201 cagtgaaaga gtagattata taacttattc agtagataac acagttcgat gttatgagat
     7261 caaagttact ttagctgatt taaaaagcaa ggcgaaacag acttttatcg gtgattataa
     7321 ctatttagtt gttactagtg atttgtggga gaaaatgcaa caagacgata aacttatgcg
     7381 gaaatattac tatcaaggcg tatacgtgtt caatgaaaaa tatcctagca taataagtgt
     7441 taagaaagca aggaaacaaa aagtatcgct aggtacaaga tcaactgtat tagaaagcat
     7501 ggttcgttct atgaatcgtg aagttaagaa actctataaa gtaaaaccat tttgggagat
     7561 agcagaggtg gaaaaaggat gattcaaaca tttgcaaaaa tgagtagaag tgcttggatt
     7621 gaaaagctgg aaaatgattt gagtgttatg cgattagctc caaagaaatt caaacgagag
     7681 attgcagaaa aagaagcaat tttgtcggta ttgaatgcgg tagaactgga cgaaccgaaa
     7741 aagtcagttg atgtacctga aaatgtagca gagttactag attattatcg taattcgtca
     7801 gatgtcgatt tgttagctct gatattaaca tttcacgatt ggtattactg tcaaaatgaa
     7861 gatactcagc atgataaaca tgataaagca gttgactggt taatagagaa tccagaaaag
     7921 ttcatgcgtg cttggctaga tggctatgat agcaagaaaa agccactgga aagtaatgag
     7981 gaaacgtatt taacggcaga acaatatgac tatgctagaa aaacaggaga ttgggaatct
     8041 ggtaagactt tcaatcacag aaaagatcaa ccaaaaatta gtttttcaga tatagccgca
     8101 attatcgaag gtatctacta tggacaagcc gatgaagaga tcttttctat acaagattta
     8161 ttagaatttt tgaacaaaaa taaggatagc tttgtagaac taactgatta tgaggaggat
     8221 acagcaaatg gaaaattatg atccaaatat gcgatggggt acacatacgc ttaaagtttc
     8281 ttttcaacgt tgggattaca agggatttgt gacttttaga aaagctggaa attgtaaagg
     8341 attagatgtc ttagcgttgg atgaagacta tctatatgac catccattga cggataatcc
     8401 aatcggattt ggattgcttc ctgaagatga tgagggcaat gaatggttca aaatgattct
     8461 tacaaacgat aaaggtgatc aattatttgt agaagatact tggtcatatt tgagtgaata
     8521 tattgttagc attaaaatca ttgattttgt agcagataaa gaaaaagaga ttggagaagg
     8581 taaatctaat tactagctaa tgaatagcaa ctttggagga taagtcaaaa gaataaaaaa
     8641 agaatactgt gtgtcattgc agggatgtat ctttgcatga tagtatctgc ggttaccatt
     8701 tattggttag gaaatcagca aaacagttta attgttgatc tgggggagtt aaaggttgga
     8761 ttacgaatga ctagatggct tttattagct caaacagttg gtttagccat attgacttgg
     8821 atatgtgtgg aaaaaataac atctaataat aatcaagaga ttgttgaatc aacaaaccta
     8881 agaataagaa taactgaaca agcaattgaa aagcaaaaag acaaagaaaa aaaggaaata
     8941 atgtcaattt atttatctag tggagcatca ttttttcatg aaatagattc gatgaaactg
     9001 agtagtcaag aaatcattga tagatggaaa aacatgcctc taggtggtga gttaatcgtt
     9061 gaagatagtg atggttggtc agcttataag aaggacgaga ttgtagctat cacgattttg
     9121 tttgaatagt aatatattac cactagataa tctgaatatg ttgatctgct agtgacgtac
     9181 caaatgcgtt taatagaacc ttggtcacag gcagaaattg ataaagcagt aaaattgaac
     9241 cgattatcaa aatttaacgt attaggaaat gccttttaaa aatcgcaata cacaaacaga
     9301 aaaataccaa aaaattagca atgaacgtag gtaagaacat gacaataaat aataataacc
     9361 ctaacaaata taatgctctt gtccaatgct atatcgaaaa caaaattaca atgcaagaag
     9421 tcgctgatga attaggcaaa tcaaaaagcc tagtatcaca cgactttaag cacaatgttt
     9481 ttccgaaccc gattgagctg aggaaaaaat ttcaaaagag agtttatcaa gaaataggga
     9541 agtggattga tgaagggaat gcaattgacc ctttaattga aaaatatcct tttttaaaaa
     9601 gtaaaaataa aattcaaagg ttaattcgtc aaggcgatat tcaagctaaa gaacctgttt
     9661 tgacaaccaa tgatttggct aaaataaatc ttcataaaca gattattgaa gactttgaaa
     9721 aaaatctttc aacaactgaa attttaaaaa aatacaatat tccgagagga aagctaacat
     9781 cgactctttt tagagaatat ggagaatcgg cagttactaa agcaaaggaa gtagcgaatg
     9841 agagaaaaaa acaaaaatag tagtcgatac ctagtagcga ctgtagtttt tgtttgtgta
     9901 ttgattttcc tgtttgattt tttgcagatt ggaaaaagca taattggaaa tacattgagt
     9961 gcaaaggaaa tggttgcttt aggtggtttg tatgaaggac atttgttccc gaatatattc
    10021 atttctatcg tttctcatat taacttgtac catttaattt ctaatttatt gttcttagta
    10081 gttcttgaag aagcactgaa tgatcaatat gagtggttaa cactattgat tgttttaatc
    10141 atatcaggac ttctaggaag cttagttcct tttctgttac aaagcaacat cgtaagtatg
    10201 gggcttagtg gaggtatttt tggatggatt gctttatttg cgattgcata catccgcttt
    10261 gatagtaagg aacatttatt actaagtgtt tttatattat tgataatcgg cattttactg
    10321 gtatctactt ttgaatcagg cgagacaaac gtcttgtcac atgtaatcgg atttgtctgt
    10381 gggatgatct tgggatttat tctaaagaac atttcattga tcaagcaaaa gaattaaaag
    10441 ggagtgctaa aaatgccaaa aaaaaaagaa aagaaaattc cagtgttttt gtctgtctca
    10501 gaaatgttat cacttcaaat actgttaaga aaaaagattg aaacaataga cgaagaagtt
    10561 agaaataaaa gggctatgta cgaacaacgt gaagcgctaa gagaacgaat ccactatttt
    10621 gatgatatac acactaaaat ctatgaagca cgtacaaata acgaagacga aagcaaatta
    10681 ggattagctc aacaacgaat atttgaatgg ctaaatacga atactgagca agaaccgtta
    10741 agagcaatta gtaatctttt tgaatttgga acgaagcatt ggtttaacgc agtagaaaag
    10801 gatgctgact tacaagaagc ctttgacacg ctaagtaagg atagcaaatc catgcgagat
    10861 ttattgatta cttatctaaa tgagcatgag tttgaacaaa cgaaaaccaa aaagaaaaat
    10921 aaagaataga aagttgtgat gaatcatgct attaaccctg ttaggttatt tatgtttaat
    10981 tcttgctttt tttggggcaa tcgctataat tattggtcaa tttcaaacaa ataatccgat
    11041 tatgcgtaac ataggcattg gaattgtgat tgtaaccttt attgtgttta atgccactaa
    11101 gatttttgga agtaacgata aacaatttgg tatatcggga gttaataata gttatgctcc
    11161 agattctttg ggaagggtag taatagatgg aacatttgaa aatgtaacga gttataaaaa
    11221 tgtttcgctg acagtgaagg aagatggcaa gaaagcatca gacgacgttc tcgctactat
    11281 taagccgtct caatcacaat ttgaagtgtt ttatacagtt aaagagaata gccctgtaca
    11341 caagctgaca ttggttttta acgttgatgg agaagaatac tataaagatg taaaaataaa
    11401 taaaacatca acgaaaaaga ttatcgaatc agcgaaaaaa gaagtagcta cagaagaaag
    11461 tacaactgaa tcaagttcta ctgtcgaagc ggaactacca gataaaattg attttgatcc
    11521 tgaaaaagtc aaatcgtttg aatctgtttc tggttctatg gctgatcggt ttaacactga
    11581 agtcttcaaa gctatcaaaa ctgttaaatt ggatactgaa aagcaaatga ttaatgttac
    11641 cttattcaat tcagttgaat ctatggatca ggataaaaaa gttacggttg caagagaact
    11701 aggaaaaaga gtaattggtt tgtatcgtgg tactgctaaa ccagattcag acggtgcaga
    11761 tttgccagtt catgtagttt atgaagacgg taaagttttt ggagattcta cagcaaacga
    11821 agcattaacg attaaattgg ctaaatagcc agaaagttat aataaacaaa tggtgtcaca
    11881 ccaataatat ataggtgtga caccttggag atgatagctg atggtagtaa ataaagaaaa
    11941 agttagaaag tatgtgactt taaaaatagc ttctgttaaa tatttagaaa agcgagctaa
    12001 agaagaagaa agagcaagag gtaagaaagt aacagtttca gagttgttag aagaattgat
    12061 cgagaatgat caaacaataa gacgtgcatt tactaatagg tctatcagat aacaatataa
    12121 ttaataaaaa agaaagagtg atttggtctt agactaaatc actcttttta tataaagttt
    12181 ctttacactc attccgaata atgttaaaat tctttatatg atatatagtt ccttgtcaga
    12241 ggaagagata accagcaata ttggagggat aataagtgga aaacaataat gatattagac
    12301 attttagcat tggggaaaga gttacaacag taagtgaatt atcaaaggat tatagagata
    12361 aagagggaac gattatttct cttaataaga atcgaagagg cgtttggcaa tatgttgttg
    12421 atttaggtga tggcgttaca gttgagtgta aagatagtca actgatcaga aataatggca
    12481 gtcattccat tgaaaaagca attcttgtat acccaaaagt atcaagtaag atcatgaatg
    12541 actggttgct tggaaaattt ggtgagtaca ataaaaaaga aatcgcagaa gtgatcggca
    12601 aatcttatcc aacagtatta gggtatctaa aaaagataac gagtaaagaa gcaaaaattg
    12661 aggatgtttt aacagtaaga gagcttcaat cattaatcga gtatcggaat aaacaaataa
    12721 caaatcctga gtaccttaga aaagaagatg gtctattgaa acaattggaa caagtaaata
    12781 aaatccggaa gatgcaaaag gtagctcctt taagccttga tacctttaaa aaaatctatt
    12841 tgaaagagat caaaaaagac taatgtggac tgtaacttta ccgttccaac ctgtatctgc
    12901 accaagacca agtgcaagaa ggaatgctgc tgatgaaact aatgacgtag cagagaaaag
    12961 gatttcaacc tactatgata aaaagtatct tgattatttg aaaaaaataa agagctttat
    13021 cgaagatcaa gatttattag atgatgaatt ttactcgatt gtaaatgatc ctatgggcgc
    13081 aattatggaa gtggactttt actaccagat accaaaaagc tataaaaaag tctataacat
    13141 tatgaagaca acagcgccag atttagataa tttagtaaaa agtgctatgg atggtatttt
    13201 taacatctca gctatacgag atagccatat cacaggctta attgctttta aatataatgt
    13261 ggcgaacgaa ggaaaaacag ttgtgaggat caaacgatat tcagaatttg agtgggatac
    13321 ttctgaagga ttgaacggag atatatggga agctacattt gattttaaac cagtagcaac
    13381 gccaagacca aagtctaaat ttagaggaaa tgggattatc acttactatc caaaagagta
    13441 caacgactat ttggaaaata tcaagaatac gcttaaagag aaagatttgg taaatgatca
    13501 actgaaaaag gtaatgaatg ctcctatggg ggttatagct cagatagatt atttttacca
    13561 agtgagaaaa gataagaaaa agcttgattc actgatgcgt acttcacagc cggatattga
    13621 taatttagtc aaaggaacat tggacggaat ttttaacaag gaaataggca tcaaagatag
    13681 cagggttgta gggttaattg cttttaaata caatactttt gaaaagtcga aaacaaacgt
    13741 gagactacag gagatgggct gatgaataat aatgaaaaaa ttcttcatgt acttgatagc
    13801 tttgaaataa ttcaagaaga actaaaaaaa tatcgggatg ttttggaaca aagatatgat
    13861 tttgtcaatc agcaaaagag taatcacatg gatttcattc tcaatatgaa tgacttgaag
    13921 aagaaattag ttgaacgaaa agaacaagaa aagttgatca aagcttactt tgaactcggt
    13981 gaaaaagaag ttaaaaatgc aatggaactg aatgaagagc gcagggtttt agatcagctg
    14041 ttagaacaat tattggtgat gtttcaaaaa ggacgaatag atgaggattt aattgaagaa
    14101 ggattgagaa aatatccttc aaactcaggt atcggaatag tgctgaaagc gattgatgaa
    14161 gaagaaattg aagctttcat accgccagag gattttgaat cggcaatgga atatataaaa
    14221 tattatagcc aagggatcac tgcttttaga gagtttgatc ctgaagatgt tattcatgac
    14281 ttgaacaacc taaaagaatg gtgtgaaagt tatggagtcg atgattccgg attggactac
    14341 ttgatcagta ttatggaaat cgaagaagag atgccggata agcctgatcc aacagatatt
    14401 ttagaattga tccatgaagc tagaaatcca attgcctata tttctagagg atatactgta
    14461 ttggaatact ataagcctta tatatcagcg atgaaccatt taagacgtgt attaagggaa
    14521 aaacgtgaat accgctcagt gttgaatgca acgaatagat tagaaaaagc tgtttcagag
    14581 ctagatgctt attatcgtga acattacctg caagcaggtg gtatgccaag aaatacgaaa
    14641 gcaaatatca gtaggtatat aaaaaaagct gagtaggcgg agttagccca taaaatggca
    14701 aaaggtaacc tgaaaggggc attttgaatg aaaagggatg tttttgaatt gtacgtatta
    14761 gaacgtctag aagacggcat gatggcacaa gagataattg atgaaattga tgatatgatc
    14821 agcagatttg gtcaattgga gttgaatgat ggaaacaaaa attcggtagt gctaaaacaa
    14881 ttttggttga acaatttttc gtataaggat tttatcaaag aaaaggcaaa tacattgtac
    14941 tgactaaatt cacgaggagc agtgatgaga gcttattgct aagagcagtt aggctctttt
    15001 ttgtttgtgt aaaattattt gttttataat acacatttat acacaaaaaa atattgacag
    15061 atagtacaca aatgtggtag gatatatttg taagcaagtt agattaacaa gttaaaggaa
    15121 gagcagttat gaaaaaagaa gaactaatag atatatttca aattgttgaa cgagcaaata
    15181 atatgggaat tatgtttttt gatcgtattt ctttaaagat g
//
LOCUS       DANKBF010000069        15072 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000069 DANKBF010000000
VERSION     DANKBF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            78..686
                     /locus_tag="PWY89_002329"
                     /pseudo
     CDS             78..686
                     /locus_tag="PWY89_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="PWY89_002330"
     CDS             706..1734
                     /locus_tag="PWY89_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2067336.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="PWY89_002331"
     CDS             1731..2489
                     /locus_tag="PWY89_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2067337.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="PWY89_002332"
     CDS             complement(2575..3315)
                     /locus_tag="PWY89_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2067338.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="PWY89_002333"
     CDS             3376..4629
                     /locus_tag="PWY89_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2067339.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4930..5178
                     /locus_tag="PWY89_002334"
                     /pseudo
     CDS             4930..5178
                     /locus_tag="PWY89_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="PWY89_002335"
     CDS             5300..5626
                     /locus_tag="PWY89_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2067340.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="PWY89_002336"
     CDS             5694..6407
                     /locus_tag="PWY89_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2067341.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /locus_tag="PWY89_002337"
                     /pseudo
     CDS             6717..7569
                     /locus_tag="PWY89_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7690..9555
                     /locus_tag="PWY89_002338"
     CDS             7690..9555
                     /locus_tag="PWY89_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2067342.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9603..11012
                     /locus_tag="PWY89_002339"
     CDS             9603..11012
                     /locus_tag="PWY89_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2067343.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11120..11821
                     /locus_tag="PWY89_002340"
     CDS             11120..11821
                     /locus_tag="PWY89_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2067344.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11980..12459
                     /locus_tag="PWY89_002341"
                     /pseudo
     CDS             11980..12459
                     /locus_tag="PWY89_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13307..13882
                     /locus_tag="PWY89_002342"
     CDS             13307..13882
                     /locus_tag="PWY89_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL2067345.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="PWY89_002343"
     CDS             13882..14322
                     /locus_tag="PWY89_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2067346.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="PWY89_002344"
     CDS             14338..15039
                     /locus_tag="PWY89_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2067347.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5083 a         2501 c         3179 g         4309 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa tt
//
LOCUS       DANKBF010000070        14625 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000070 DANKBF010000000
VERSION     DANKBF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            4..816
                     /locus_tag="PWY89_002345"
     CDS             4..816
                     /locus_tag="PWY89_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL2067348.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="PWY89_002346"
     CDS             813..1613
                     /locus_tag="PWY89_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2067349.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="PWY89_002347"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="PWY89_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2067350.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="PWY89_002348"
     CDS             3110..4819
                     /locus_tag="PWY89_002348"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2067351.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="PWY89_002349"
     CDS             5031..9389
                     /locus_tag="PWY89_002349"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2067352.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="PWY89_002350"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="PWY89_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2067353.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="PWY89_002351"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="PWY89_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2067354.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="PWY89_002352"
     CDS             11227..11520
                     /locus_tag="PWY89_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL2067355.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="PWY89_002353"
     CDS             11517..11828
                     /locus_tag="PWY89_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL2067356.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="PWY89_002354"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="PWY89_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2067357.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="PWY89_002355"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="PWY89_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2067358.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DANKBF010000071        14575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000071 DANKBF010000000
VERSION     DANKBF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(510..1076)
                     /locus_tag="PWY89_002356"
     CDS             complement(510..1076)
                     /locus_tag="PWY89_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2067359.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="PWY89_002357"
     CDS             complement(1076..2032)
                     /locus_tag="PWY89_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL2067360.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="PWY89_002358"
     CDS             complement(2032..2688)
                     /locus_tag="PWY89_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2067361.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="PWY89_002359"
     CDS             3058..5076
                     /locus_tag="PWY89_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2067362.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5146..5352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5503..7917
                     /locus_tag="PWY89_002360"
     CDS             5503..7917
                     /locus_tag="PWY89_002360"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2067363.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="PWY89_002361"
     CDS             8265..9818
                     /locus_tag="PWY89_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2067364.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="PWY89_002362"
     CDS             complement(10199..11983)
                     /locus_tag="PWY89_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2067365.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="PWY89_002363"
     CDS             complement(11998..13719)
                     /locus_tag="PWY89_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2067366.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..>14575
                     /locus_tag="PWY89_002364"
     CDS             13819..>14575
                     /locus_tag="PWY89_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2067367.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMK"
BASE COUNT         4727 a         2719 c         2878 g         4251 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaa
//
LOCUS       DANKBF010000072        14353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000072 DANKBF010000000
VERSION     DANKBF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..135
                     /locus_tag="PWY89_002365"
     CDS             <1..135
                     /locus_tag="PWY89_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2067368.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="PWY89_002366"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="PWY89_002366"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2067369.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="PWY89_002367"
     CDS             complement(2412..2663)
                     /locus_tag="PWY89_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL2067370.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="PWY89_002368"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="PWY89_002368"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL2067371.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="PWY89_002369"
     CDS             3613..3831
                     /locus_tag="PWY89_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067372.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="PWY89_002370"
     CDS             complement(4120..4632)
                     /locus_tag="PWY89_002370"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2067373.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="PWY89_002371"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="PWY89_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2067374.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="PWY89_002372"
     CDS             complement(7085..7528)
                     /locus_tag="PWY89_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2067375.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="PWY89_002373"
     CDS             complement(7529..8317)
                     /locus_tag="PWY89_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2067376.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="PWY89_002374"
     CDS             complement(8314..9255)
                     /locus_tag="PWY89_002374"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL2067377.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="PWY89_002375"
     CDS             complement(9432..10019)
                     /locus_tag="PWY89_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL2067378.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="PWY89_002376"
     CDS             complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="PWY89_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL2067379.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="PWY89_002377"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="PWY89_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2067380.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="PWY89_002378"
     CDS             complement(12936..13871)
                     /locus_tag="PWY89_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2067381.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14057..>14353
                     /locus_tag="PWY89_002379"
     CDS             14057..>14353
                     /locus_tag="PWY89_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2067382.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         4021 a         3190 c         2528 g         4614 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataacc cgcgaattgt aaaattaagt
    13981 tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa
    14041 aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga
    14101 atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat
    14161 acaaactatt tataatgtag tcaataagtt caagcaaggc aagactgctc ttgattattg
    14221 gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat tacctgctca
    14281 tgaagtagat tacattaaag agaaagtcac tcttggttgg acgcctgacg tcattatcgg
    14341 gcgaaaagaa agg
//
LOCUS       DANKBF010000073        13995 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000073 DANKBF010000000
VERSION     DANKBF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="PWY89_002380"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="PWY89_002380"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2067383.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1708..2025
                     /locus_tag="PWY89_002381"
     CDS             1708..2025
                     /locus_tag="PWY89_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2067384.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2229..2594)
                     /locus_tag="PWY89_002382"
     CDS             complement(2229..2594)
                     /locus_tag="PWY89_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067385.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2802..3284
                     /locus_tag="PWY89_002383"
     CDS             2802..3284
                     /locus_tag="PWY89_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067386.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3481..4371
                     /locus_tag="PWY89_002384"
     CDS             3481..4371
                     /locus_tag="PWY89_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL2067387.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            4696..7167
                     /locus_tag="PWY89_002385"
     CDS             4696..7167
                     /locus_tag="PWY89_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2067388.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7270..9006)
                     /locus_tag="PWY89_002386"
     CDS             complement(7270..9006)
                     /locus_tag="PWY89_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2067389.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9003..9707)
                     /locus_tag="PWY89_002387"
     CDS             complement(9003..9707)
                     /locus_tag="PWY89_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2067390.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9838..10341
                     /locus_tag="PWY89_002388"
     CDS             9838..10341
                     /locus_tag="PWY89_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL2067391.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10301..10639
                     /locus_tag="PWY89_002389"
     CDS             10301..10639
                     /locus_tag="PWY89_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL2067392.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10660..12078
                     /gene="ffh"
                     /locus_tag="PWY89_002390"
     CDS             10660..12078
                     /gene="ffh"
                     /locus_tag="PWY89_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2067393.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12190..12768
                     /locus_tag="PWY89_002391"
     CDS             12190..12768
                     /locus_tag="PWY89_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2067394.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12772..13293
                     /locus_tag="PWY89_002392"
     CDS             12772..13293
                     /locus_tag="PWY89_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2067395.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13372..13926)
                     /locus_tag="PWY89_002393"
     CDS             complement(13372..13926)
                     /locus_tag="PWY89_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2067396.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         4656 a         2403 c         2789 g         4147 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatcttttta
     1621 cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat tttttcggta
     1681 tttaatattt aattaggagg gaatattttg cttttttata gtaattttat cttaatagta
     1741 gcgatattat tacttctgaa tatatggatt tttgatagga gtagaaatgc aagtataggt
     1801 tttagaacta aacgaagtct gtcttctaaa aaaaattggg tatactcaca aactattttt
     1861 tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt gaatattata
     1921 gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc aattattaca
     1981 caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa ctagtgatag
     2041 ttttggtgat agtgatatta aaaatcagaa aagatagaaa aagcaggaga tagataaaat
     2101 gctgatattt cagcattttt taagagtata aaagagaaaa aaagtaataa atgtatacat
     2161 gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg tacatatatg
     2221 gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca taacgaccca
     2281 aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt cggttttaaa
     2341 ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta
     2401 gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa ataatatcca
     2461 gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata acgaaaatca
     2521 agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag gtttttccag
     2581 caagttttta tcatctttca tcaccaccta acaagtgtta ttattttgtt agttttattg
     2641 taaattgaat cctgcaataa tcaattactt tttcattatt tctagattaa atgaataaac
     2701 tctcatgcca ttatctctaa aaaaaaagtt tttcccccta atttttttag atagaaaatt
     2761 gtatagtatg atgagagaaa aagtagtttg ggggaacgtt aatgagaaga aaagcagtaa
     2821 tcgatcggct ttatttattt ttttggtttt ttcctatcgt tttgctacct tttacatggg
     2881 taaatgctgt tgtacaaggt gcggagatat taggcctgat ccaaggaagc ggatttgtgg
     2941 tttctaggaa ttttccagct ttgactgttt tctatttagt gataagtgta ggtctcttat
     3001 taccaaaatc tctgatcaat cactacaatt ggcagctgct ttacttttct tgcttattag
     3061 tatttacatc tatgttttct ttgctgcctc gcttgattat cggccctaat ggtttgagcg
     3121 agagtggatt gaaggattca gcttattcct ccatgtattt cttgacgatc aaaccatggt
     3181 tctatttagt aattgcctca tttattcttg caacattttt ttacttctgg acagaaatca
     3241 ggataaaaag tgaacgatct gtatctataa aaaataacta ttgatggtgg atcatcagta
     3301 aaaaagcata tgagctgcgt aaaatgcatg tttctcatat gcttttttta ttattatgta
     3361 aagaatgcga aaaacgttta catgattttt acataactta actatatttt tacagcaacc
     3421 atacgaggga tttacagtcg actgataaac tttagttgta aataagaaag gagcaataaa
     3481 atgaaaaaag tattgtttac tctaggtatg atgggaattc ttttagccgg atgcggctca
     3541 ggatcaggaa atacagatgg ctctgccaca caaaataatt caagtgactc aaatgaacaa
     3601 gtaaaaatcg ttgcggtagg ttcgacagca ttacagccgt tagtcgatgc agcacaagaa
     3661 agctttgtac aagaaaatcc aaattatcaa atctctgtac aaggaggagg aagcggaact
     3721 ggtttgagcc aagtcgaagc tggagcagtt actatcggaa actctgatgt atttgcagaa
     3781 gaaagagacg gagttgatgc ttcaaaatta gtggatcata aagtagcagt ggtcgggatg
     3841 gcgccaatcg tcaataaaga tacagatgtc aaagatatca ccaaacagga attgattgat
     3901 atctttacag gaaaaatcac taactggaag gaagttggcg ggaaagatca aaaaatcaac
     3961 gtcgtgaacc gtgcaaacgg cagcggtacc cgcgcgacat tcgaaaaatg gggactggat
     4021 ggtgctaccc ctgtgcagtc ccaagaacaa gattcatcag gaacagttcg gcagctggta
     4081 agtcaaacac caggagcaat cagctattta gctttctcat atctcgatga ttccacacaa
     4141 gcactaagta tcgacggtgt agaaccaaaa gaagagaatg tagcagataa cagctgggga
     4201 atctggtcat atgaacatat gtacacgaac ggaaaacctt cccctgaagt ccaaaaattc
     4261 ttagactaca tgatgacaga agaaatccaa gaaggacctg tcaaagaatt gggctattta
     4321 ccaatcacaa tgatggaagt agaacgtgat cacgaaggaa atatcaaata actagcagat
     4381 aaacatgcca cggtagtcgt tactattaag gaaaaatcaa ctatgtttcc cacatataca
     4441 tttttccttt aattgtgaaa aatagttcct catattttgc cgttttttga gctaataccg
     4501 agggtcagct gattaaacac cgtttattcg aaaaaaagag ctacgacata gaactgtcgt
     4561 agctcttttt ttgctatatg acaggaatga aaggtgtttg caagcttaat gtgaggaaca
     4621 ctttccatat ctgttctata ctggatagga gaagtttgga aatatccaga aaacgaaatc
     4681 aatcgaggta gccacatgaa gaataaatgg ataaatcgaa gtaaaaaaaa gaaagtaaca
     4741 caaagatcat cagataaagt catccgtctt tttccatcgt ataaagaagg attaacggca
     4801 gatcaagtca aggaacgaat agaaaaaggt gcagcaaata attcagtcga tcccacattc
     4861 aaaacaaatc agcaaatcgt attggaaaac atatttacgt atttcaattt gatctttctt
     4921 atcttagcca ttttgctttg tttagtagaa tcctataaga acttgacttt tttaccagtt
     4981 atcatagcca atacaggaat cgccattttt caggaaattc gatccaaaaa gatcttggac
     5041 gaactaaatg ttttaaatac agcaaaagtc aatgtcatac gggacgggat agaacaaaca
     5101 atagatatag aagaccttgt aatagatgac attgttattt tagagactgg acatcaaatt
     5161 ccagcagatg cagaggtcgt ggaaggaaaa ctgcaagtta atgaggcttt attgacaggc
     5221 gaagcagatg aaataaccaa agaaataggc gatccattaa tgtccggcag tttcattgtt
     5281 tccggaaaag cttacgcccg attagacaaa gttggagcag attcgtatat ttcccagctg
     5341 acagtcaaag ccaagacaat gggagaagga gaacagtcgg agatgattgc ttctcttaat
     5401 caattgatca aatggattgg gatcatcatt attcctatcg gtatcctgct tttttctcaa
     5461 agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg ttgcaatgga agcagcattg
     5521 atcggtatga tacctgaagg actgtacttg ctgacgacga ttgcattagc attaagtgcg
     5581 actaaactag caaagcaaag agttctttta cacaatatga aaagcattga gacattggca
     5641 agggtaaatg tcctttgtgt agataaaaca ggaacgatca cagaaaataa aatgtctgtt
     5701 caaaaagtga tcgtctctaa aaaacaagaa ataatcagtc agtcagatca attggaagca
     5761 tgcattgctg actatgcaaa agcgatggca ggagacaatg caacaatgga agctgttaaa
     5821 gcatatttta caaaaaccac tgatcaatcc tatcaccatg ttattccttt ttcttctgtc
     5881 caaaaattca gcagtgtagc gttggcagac aaggtgtatg ttttaggagc gcccgaaatg
     5941 gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg tttctttcgc tgaaaaaggc
     6001 tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg aagaaccagt attgacagaa
     6061 gcagtcacac cattaggcta tattctgatt gctaatccta tcagaaaaga agcaagagcc
     6121 acatttgatt acttcaaaaa acagcaagta gccatcaaag tgatttctgg agataatccg
     6181 ctcaccgtat ccaatgtagc tgtccaagca gggatttcac atgctgatca gtacgttgat
     6241 gcatctaaat tatcagaaga agaatatgaa gcagctgtcg aaaaatatac agtttttgga
     6301 cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga taaagcgtaa caatacagta
     6361 gctatgacag gtgatggcgt gaatgatatt ctagccatga aagaagcaga ctgcagtata
     6421 gcgatggctt ccggaaatga agccgccatg caggcctcac aagtggtctt acttgattct
     6481 gacttttcac gcatgccaga agttgttgca gaaggacggc gcgttgtgaa caatattgag
     6541 cgatcagcca gcttgttttt agtgaagaat attttctcct ttttgctctc actgttttca
     6601 gtgatctttg ctctgaccta tcctttagaa ccatcacaaa tcacattgat tagtttgttt
     6661 actatcggac ttccatcttt cttgcttgct ctagaagaaa acaaaaagcg aatcagaggg
     6721 aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg gactaacaga tatgatcgta
     6781 gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa ataaaacaga tgtttctaca
     6841 gcatctacca tgcttttgat tgcagtaggg ttcctcgttc tctataaaat ctgttcaccg
     6901 ctgaataaat tcagaagtca gatcattctt ttctgtgcta gcggtaactt tttttcggtc
     6961 atctttttgc ataagctgtt ttcaatcacc ggtatctcag ctgtatcact tttgctgttg
     7021 agcattctat ttttctcggc agattcgatt tttaggcatt tgaccacatt ggtagaatat
     7081 ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta gtcgcgtagc taaaaaattt
     7141 ctatcgatat ttaaacgaga aaattgacga aaagtatctc aaaaaagagt aaaaaagaac
     7201 aaccactcgt gtatctttcg aagcaggatg agtcatccaa ctttaaaacg cacgagcagt
     7261 tgctcttttt tacattttcg gaatggtaac ggtgaaagtc gaacctgttc caagatgact
     7321 gtctacagta atcgtaccat taaggagttg cacgtagtct tttacaatcg ctaaccctaa
     7381 tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg cggtaaaaac gctcaaagat
     7441 ccgttcctga tcctcaatgt cgatacctat accaaagtct tggacagaaa acgatagatc
     7501 atctgttgcc tgatagcgga taataatctc gctgtcgctt tttgaatatt ggacagcatt
     7561 ttcaatcaga tttttgataa ttggataaag gatatctgtt ttagtagtaa atggttcgtt
     7621 ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg attggctgat aattttgaac
     7681 gatttgctgg aaataaggtt ctgtataaat tgtctggatc tcgtaagaat tctcgccatc
     7741 ttttgacagc tggatgattt cccgaatcag tttatctaac cggatcgcat ctttttgcat
     7801 gatctcaaga aaagaagtca gagtttgcgg gtcatctttt gcaccatcca gtaaagtctc
     7861 ggtaaaacca atcagtgaag tcacaggagt ttttaattcg tgagagacgt tgccaacgaa
     7921 atccttttgt agcttttcta aacgtcgcac tttcgttaag tcatacgcca cccctagaat
     7981 ctgtccggta ccttctgaat tagtaaagta gcgcaaactc aagtctaatg tctgccgacc
     8041 ttctgtaagg gtgatctcct gatggatcag cggatgctca ggagtcactt gatgaatcag
     8101 ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc tgttcaggtt gatagcttac
     8161 cacgtttaaa tgtagctgca tctttggatt aagcagtagt aaacgagaat cagcatcatc
     8221 aatcaggaat acgccaatca tcagttcgtt cagtaacgta tataattgct cttctgtggt
     8281 agtacacgcc cgatacatct tactcatttg ttcgctcaac gcattaatcg tctgatacaa
     8341 ttcttcccat tgatcgggtg tttgcatgat tacttccgcc tgattaggat tttgcaccat
     8401 tttttttagt acgggcagca cggttttcaa tggctggttt ttttgataga tcagataata
     8461 gatcattcct gcaagaacaa ggaagaaaga cagaaagaag aaaaagaccc aacggcgaaa
     8521 actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg atacgtaaaa tggcttctaa
     8581 ctggccactt tttttgactg gtaatgcgac atataaaaga tcctcattca aagtagtact
     8641 tttgcgcaaa gcgctaccta ggttcccacc attcaatacg gctttgattt cgggacggga
     8701 gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat aagatctttc cagtagcatc
     8761 cattaaagta atccgttcgt tcgactgatg gacgaattct tccaaagcag ttttgttttg
     8821 cgctgcttgg aggttttcga catctaattg ccggatcagc agttgtgctt tttttgtcag
     8881 gtagctttct tgctgttcca cgatctgctg ttgaaaataa tgggaaatca attgccagcc
     8941 accaaaaaac aaactaacta aaagaataag aaagaagaga cgttcataaa tttttttcca
     9001 tttcatcgtt ttggttcctg aaacttatag ccaaatcctc taatagtcac caagtacttt
     9061 ggatgttttg gatctcgctc gattttttct cgcaaatgac taacgtgtac atctacgatt
     9121 cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta gcaatgtgtc tcggtcgatc
     9181 acacggtctt ttcgtttcat gaaataaaca agtaattcaa attctttcgg tgttagttca
     9241 atcagatggt cgtctacggt cacttgataa ttggtcaaat cagcagtgat ttgtccaatc
     9301 gatagatatt caggctcagc ttcatctttt gattgcttac gaggttcaat ccgtctgaat
     9361 attgctttca tacgggctaa tacttcgcga ggactaaatg gtttggtcaa atagtcgtca
     9421 gcacctattt ctaggccaat gatccgatcc acttggtcat ctttagccgt cagcatcaaa
     9481 atcggtgttt catttttttc ttgacgcagc cgctgagtga tctccatgcc atccatcgtt
     9541 ggaagcatca catctaaaat gatgaaatca aatgattgtt ctaatgctaa ttctaatcct
     9601 tcgcctccat ctgtggcact ggttaccttg tagccttctt tttccaaatt gaacgtcaag
     9661 agcgttacaa tagaaggttc atcatcaaca actagtattt ttttcattca aatctccttt
     9721 aaataggttt cattctgttt atattttagc atagaatcag tacaaagggc tattttacaa
     9781 aagaaataaa tggtataata ggaaggcact ttgctgaata gaaaggaaga gcagtcaatg
     9841 attggaatca gtgcatgttt aggcggtctg gcctgtcgct atgatggaca gaaaaaagaa
     9901 gtaccagaat tgatggaact agttgaaact ggagaagctg tcatggtctg tccagaagtg
     9961 atcggcggat taccaattcc tagagatccc gctgaaatca ttggtggaaa tggattcgat
    10021 gtgtggaaag atcaagcaaa agtatggacg acaagcggcg aagatgtaac ggaagcgtac
    10081 aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa aacacattga tctagtcatc
    10141 atgaaggaaa aaagcccttc atgcggaacc cattcgattt atgacggaag tttttccgga
    10201 ataaaaaaaa atggtgccgg agtggctact gcttatttta ttcagcaaaa aatgactgtg
    10261 ctctctgatg aagaatggct gaaaactaga ggtgagcaga atggaaatcg aaaagaccaa
    10321 tcgaatgaac gcgctctttg aattttattc gaccttattg acagaaaaac aaatgaacta
    10381 catggaatta tattatgcag atgatttttc attaggtgag atcgcggaag aatacgaagt
    10441 aagccgacaa gcagtatatg acaatatcaa gcggacaagc aagatcttgg aggattatga
    10501 aaaaaaactc catctttttt ctaattatat tgttcgagaa caggtattgg aaaatatgac
    10561 tgcatatatt acagataagt acccggaaga taaaaaacta cttgaatatg tacagcaaat
    10621 tcaagcaatc gaagaataaa taaaaggaat gaagaaaata tggcatttga aagcttaact
    10681 gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag gaaaagtatc agaagctgac
    10741 gtaaaagaaa tgatgcggga aatccgcctt gccttactag aagccgatgt taacttgcaa
    10801 gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg taggggcaga agtgctggaa
    10861 agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg aagaattgac gatcacactt
    10921 ggttctgaaa cagcagagtt aaataaatca ccaaaaatac caactgttat catgatggct
    10981 ggtcttcaag gggcaggtaa aacgaccttt actggtaaat tagccaatta tttaaagaaa
    11041 aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat atcgaccagc tgcgatcgac
    11101 caattgaaag tattaggtca acagctagac gttcctgtat ttgatatggg aactgaagtc
    11161 agcccggtag agatcgtccg tcaagggatg gagttagcaa aagaaaagaa aaacgactat
    11221 gtattgatcg atacggcagg acgtctgcat atcgatgaaa cattgatgga tgagttgaaa
    11281 caaatcaaag aattgacgca accaaatgag attttgcttg ttgtcgatgc catgactggt
    11341 caagatgccg tcaacgtagc tgatagcttc aaccagcaat taggtattac aggggtcgtc
    11401 atcactaaac ttgatgggga tactcgaggc ggggcagcac tatcgatccg ctcagtgaca
    11461 ggcgcaccaa tcaaattcat cggttctggt gaaaaattga ctgacttaga agtattccac
    11521 ccagatcgta tggctagccg tatccttggt atgggggata tgctgacatt gattgaaaaa
    11581 gcgcagcaag actacgacga gaaaaaagcg gaagaacttg cacagaaaat gcgggaaaat
    11641 tcattcgatt tcaatgactt tattgaacag cttgatcaag tgatgggtat gggcccactg
    11701 gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc ctggaatcga aaacgtcaag
    11761 gtagatccaa aagatgttga acgcaaaaaa gcgatggttt attctatgac gccagcagaa
    11821 agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc gaattgctgc cggttctgga
    11881 aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca aggaatcaag aaaaatgatg
    11941 cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg accaaatgtt tggttcaggc
    12001 gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga tcaagaaaaa taagaagaaa
    12061 aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta cagactgaaa atgtctactg
    12121 ccggagcttt tctatcagca atttttattc tttactaata caattactct aaacgaagag
    12181 gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa gtatgcggca atatcatgat
    12241 actgtctggg gtgttcctga atatgatgac caaaagctgt tccgaaaatt gatgcttgat
    12301 attaatcaag ccggattgag ctggcagacg attttaaata aaagtgaagc attcgatgaa
    12361 gcatatgatc gattcgatat caaaaaagtc gcaaattatg gagaaacaaa aatcagtgaa
    12421 ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa tcgaagcagc cgtccaaaac
    12481 gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt tttcagagta tctttggtcg
    12541 ttttctaatg gaaaaatcgt tgattcttct tatcattcac aagaagaagt cccaacaaca
    12601 agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa gaggatttaa atttatcgga
    12661 agcacaacaa tctatgcttt tttagaagct gtcgggatca tcaatgatca ccttgacagt
    12721 tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg gcgaataaag catgcgaaaa
    12781 atcatatttt atggtgctat aagtttagat ggctatttgg caacgaaaac ggatgatctt
    12841 caatggcttt ttgacactcc aacaggtaag caaactactt atgaagcttt ttatgaaaca
    12901 atcgatacga ccatcatggg cagaaaaact tatcaggaag caaaaaaata tttggagacc
    12961 gaagcaattt atccagagaa aaagaatttc gttttttcca cccacgcaca attacaattg
    13021 ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc taaaaaatct acaaatgaca
    13081 gaaggaaaag atatctgggt tgtgggtggc ggtcgattga tcaaaccgct attggaacat
    13141 caaatgattg atgaatggta tatccagatt acccctgtat tattagggga cgggattcct
    13201 ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc tagacacgac acgattcggt
    13261 gaatttatcg aattgcatta tgtacgaaag tagaagtaaa taaaataaaa aaggcaacta
    13321 tatgcagaaa tgtcctgtac atagttgcct tttttattac cgttatgctt tttactctgt
    13381 taatttgtta gaaaaatcta gatacgcatc taaaatcgtt tggaagaaac ttaatgttcc
    13441 ttcaacgata ttaccatttt catcaattag attcgcaacg tttccgatat atgcttccgg
    13501 ttgctgcaag gttggcacat tcaaaaagac aagtgattgg cgtagatggt ggtttgcgcc
    13561 aaagccgccg atcccacctg gtgatgcagt aacgactaaa cctggtttat gatcccaaac
    13621 actgtgtcca taaggtcttg agcctacatc taaagcattt ttcaatactg ctggcacaga
    13681 acggttgtat tcaggagtaa cgaaaacgac accgtctagt cccctcactt cctcacgaaa
    13741 acgagtccat tcagcaggag cctgttctgg agtttcgaga tcctcattgt aaaatggcag
    13801 atcatcgatt tttacaaata cactttcgta cccttcagga agcaatttcg caaaagcttc
    13861 tgctactttt ttgttgtaag aatcttttct taaactacca ataaaaaatc caattttttt
    13921 tgtcatacga tttttcctcc ttcaaactat ctataataat cttacaaaag ataagtaact
    13981 ggagcaaata aaaac
//
LOCUS       DANKBF010000074        13978 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000074 DANKBF010000000
VERSION     DANKBF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            140..460
                     /locus_tag="PWY89_002394"
     CDS             140..460
                     /locus_tag="PWY89_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067397.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="PWY89_002395"
     CDS             912..1112
                     /locus_tag="PWY89_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2067398.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="PWY89_002396"
     CDS             1259..4048
                     /locus_tag="PWY89_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2067399.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="PWY89_002397"
     CDS             4095..4622
                     /locus_tag="PWY89_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2067400.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="PWY89_002398"
     CDS             4643..5833
                     /locus_tag="PWY89_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2067401.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="PWY89_002399"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="PWY89_002399"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2067402.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="PWY89_002400"
     CDS             7966..8640
                     /locus_tag="PWY89_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2067403.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="PWY89_002401"
     CDS             8637..8978
                     /locus_tag="PWY89_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067404.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="PWY89_002402"
     CDS             9008..9367
                     /locus_tag="PWY89_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL2067405.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="PWY89_002403"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="PWY89_002403"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2067406.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="PWY89_002404"
     CDS             12286..13752
                     /locus_tag="PWY89_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2067407.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3939 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgttttt
//
LOCUS       DANKBF010000075        13613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000075 DANKBF010000000
VERSION     DANKBF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(1..144)
                     /locus_tag="PWY89_002405"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="PWY89_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="PWY89_002406"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="PWY89_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2067408.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1716..2498)
                     /locus_tag="PWY89_002407"
     CDS             complement(1716..2498)
                     /locus_tag="PWY89_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067409.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(2563..3399)
                     /locus_tag="PWY89_002408"
     CDS             complement(2563..3399)
                     /locus_tag="PWY89_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2067410.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3417..4826)
                     /locus_tag="PWY89_002409"
     CDS             complement(3417..4826)
                     /locus_tag="PWY89_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2067411.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4840..6663)
                     /locus_tag="PWY89_002410"
     CDS             complement(4840..6663)
                     /locus_tag="PWY89_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2067412.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7506..7832)
                     /locus_tag="PWY89_002411"
     CDS             complement(7506..7832)
                     /locus_tag="PWY89_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067413.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7878..8240)
                     /locus_tag="PWY89_002412"
     CDS             complement(7878..8240)
                     /locus_tag="PWY89_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDL2067414.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8277..8491)
                     /locus_tag="PWY89_002413"
                     /pseudo
     CDS             complement(8277..8491)
                     /locus_tag="PWY89_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8619..8954
                     /locus_tag="PWY89_002414"
     CDS             8619..8954
                     /locus_tag="PWY89_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067415.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9030..11027)
                     /locus_tag="PWY89_002415"
     CDS             complement(9030..11027)
                     /locus_tag="PWY89_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067416.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(11125..11400)
                     /locus_tag="PWY89_002416"
     CDS             complement(11125..11400)
                     /locus_tag="PWY89_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2067417.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11632..12366
                     /locus_tag="PWY89_002417"
     CDS             11632..12366
                     /locus_tag="PWY89_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067418.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12405..13547
                     /locus_tag="PWY89_002418"
     CDS             12405..13547
                     /locus_tag="PWY89_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2067419.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         4373 a         2681 c         2088 g         4471 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
     1441 tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc ttaaaaattt
     1501 tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg tttaatgaaa
     1561 ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa
     1621 tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat ataacaacgg
     1681 ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt tttaaatact
     1741 tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt tttcccattt
     1801 tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt actgatacct
     1861 gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc tgtatcagta
     1921 tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat agctgtgtat
     1981 gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac ttctgtctca
     2041 ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc ttctgtctca
     2101 ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc tttagtgtgc
     2161 atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct ttcaatagag
     2221 acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa aagcattttg
     2281 ttgtttacat ctacataata atcctttgac tctacttgag accttcctat acggaataca
     2341 gctttactgt ctgatatctc tataccagca tccttattat taatctgcca atctccaagt
     2401 agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact ggtagaggtc
     2461 gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc cttagcacaa
     2521 ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca cccttgaaat
     2581 atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct cttcttgaac
     2641 gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct tcactgaagc
     2701 cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg caataaaaaa
     2761 ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct ttttttcggg
     2821 aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga gcatggtttc
     2881 ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt ttccagcctc
     2941 ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat agcagccgat
     3001 ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt gaaaaacaat
     3061 gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa aaaatagact
     3121 tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct tgacgcaaat
     3181 aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta gagcatcaga
     3241 aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat cgcctttctt
     3301 tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct tatctaagca
     3361 aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg ttctctctaa
     3421 gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt ttttattcgt
     3481 tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata acgcttattg
     3541 aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc aaaaacatct
     3601 acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata atcaattcga
     3661 tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata gccattttca
     3721 gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca
     3781 gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc agctgcccgg
     3841 tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga aacatctgat
     3901 ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc aataaaatcg
     3961 ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata taatccacga
     4021 tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac atctaagaaa
     4081 gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg actggaggta
     4141 gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag attcaactga
     4201 tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat ttgaaagagt
     4261 tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg atgaaggatc
     4321 atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata ctcagtaagc
     4381 aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca accgccctgc
     4441 tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc gattccctga
     4501 ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt ggttttatta
     4561 ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt taagcctaat
     4621 tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc aacttcaaaa
     4681 ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt cccatcctcc
     4741 tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa cttctctgga
     4801 aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt gcatcaatgg
     4861 ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt cactgttggt
     4921 cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga aatcggcgtt
     4981 gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt caaagggttc
     5041 cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac cttctttaga
     5101 acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct caacaggtga
     5161 ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc ccttagaaaa
     5221 gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat tttcaacaat
     5281 gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa atccaattag
     5341 ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg cgtgaacaat
     5401 attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg aaacaaaggc
     5461 atatctgact aaaccgctca agccagcata caaaccagca attgcaccac caatcatgga
     5521 agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct cagtaattcc
     5581 cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt ttttagtttt
     5641 cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca ttcctggccc
     5701 attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg tcattgccca
     5761 agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg gaattaacca
     5821 agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca gcaacgttcc
     5881 aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca gcatcggctt
     5941 agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta cataggacat
     6001 gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag ctaataccgg
     6061 aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat ttggatgaag
     6121 caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta tggctgcccc
     6181 ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat tcaaaatgat
     6241 gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca aaatggcttt
     6301 taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag aaatgacact
     6361 aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt taattgaagt
     6421 tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa tcaccacttg
     6481 gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta aggctttcgt
     6541 atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat gggttaagct
     6601 gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt ctctgtaatc
     6661 cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca
     6721 ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca ataacgttat
     6781 tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa tttaattttt
     6841 tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa ctagggtata
     6901 tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca gtaaaaccat
     6961 tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg catataacgg
     7021 atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt gagtaaagga
     7081 tagaataaac agcattccaa caatataggc aacaatagtg atacttgttt ttttggtaaa
     7141 aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatcg atagaatgat
     7201 ttgggctagt attcttaaaa attaactagc aaatgtttaa actgatttat ttctaatcta
     7261 catgtagtaa gtatttcaat tccaccaatt atcaataaaa ataccaaaca tatccataat
     7321 aattttttct ttttcatttc ttttatattg ttataacagt gaataacata aaaattcccg
     7381 acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag gaaactataa
     7441 aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg cataatacat
     7501 ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat acggtctttc
     7561 tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat agctttagcc
     7621 cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga tcgatttaag
     7681 ttgtactgtt cttgtaaaat ctgtgttacc aatggtttcg atgacttctc taatccagat
     7741 gtacctgtca tttttgaaaa aatgatacct ataatagcca agacaacgat aactataatg
     7801 ataaacttct tgttcatatt tactttattc aactttttat ctcctatctc caaaataatt
     7861 tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa gattgctcca
     7921 gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttatctcg atataagtag
     7981 cccataataa cagcaacagc accaaataca acaggaataa atgctaacgc tactaccata
     8041 gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc
     8101 ttcatttcat taattgtctg ctcagtactt ttctttactg cgtctgctcc agttgtctca
     8161 tctgtttctt gtagtgctcc gcaaaactgg caatacttac tttcatctgt tatttctttg
     8221 ccacattttc tacaatacat gtcttctcct cctaaaaaca ttcaattgtt tattagttaa
     8281 ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact taatatctca
     8341 gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca
     8401 cattgctgtg taacattcat ttttttgcta actccgtttg agaaagtcct ttttcttttc
     8461 tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt attatataca
     8521 acaatatgat gtatttcaac tcataaaata taaaaaaact tcaaatacca ttacaaatac
     8581 aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa caatatgcaa
     8641 aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa
     8701 tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag cctaacctcg
     8761 atagcttaac cgctctttct agagccctag gaacccctgt aagccatttt ctagacaatg
     8821 ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc actgaagatg
     8881 cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc
     8941 tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga
     9001 aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc ttttctcttc
     9061 cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaagagg aactaattca
     9121 actcctgata atatcaattc ttttattact tcatctatcc ctgctccttt tgctaggtag
     9181 actaaaaaac tactacttgc tttcacattg tatagtctct taatatcctt agaagctcgt
     9241 tcaagagtga atccttgttt aacaaagtag cctctgtttt cttttaaagc attttccacg
     9301 ctgttattcc caacattacg tagacgttca caaacagtgt tagtaagttc tttctctgtc
     9361 attagatatg ttccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc
     9421 aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct tttagcctct
     9481 ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaaat
     9541 ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa
     9601 acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc tttctcttcc
     9661 tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt
     9721 atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt
     9781 ttaccatagt aaaaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca
     9841 ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc
     9901 atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg
     9961 tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg
    10021 ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca
    10081 actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc
    10141 ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt
    10201 aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc
    10261 tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct
    10321 tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag ttcatactct
    10381 ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca
    10441 gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca
    10501 aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag
    10561 tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca
    10621 atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa
    10681 ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact atcatcttta
    10741 ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt
    10801 actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata
    10861 aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta
    10921 tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt ctctaataat
    10981 ttttctttat ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc
    11041 ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta
    11101 tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc
    11161 agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac
    11221 atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat
    11281 atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat
    11341 ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat
    11401 atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt
    11461 tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt
    11521 tcatcgacat acacataata cctcgataaa aaagaggtgt caatatcttt gtaaaattta
    11581 tttacaacac ccctttttcg aggtatacta attttaggag gtgtggtcaa tatgataaaa
    11641 cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata
    11701 agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta
    11761 attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga
    11821 ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta
    11881 gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca
    11941 tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat
    12001 ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct
    12061 aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata
    12121 gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact
    12181 agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa
    12241 gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa
    12301 caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc
    12361 atctagtggt aagtactcct tgtaaactaa gaggagatga tttcatggca aaaatatcaa
    12421 agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc
    12481 cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc
    12541 aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg
    12601 atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag
    12661 cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta
    12721 agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca
    12781 gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg
    12841 ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag
    12901 agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt
    12961 ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta
    13021 tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac
    13081 tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga
    13141 aaacaagagc ttctagacga gttatctctt tagatactga gactctttct atcctcaaca
    13201 actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag
    13261 agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt
    13321 ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac
    13381 atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat
    13441 tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac
    13501 ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa
    13561 aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta tat
//
LOCUS       DANKBF010000076        13429 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000076 DANKBF010000000
VERSION     DANKBF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..914)
                     /locus_tag="PWY89_002419"
     CDS             complement(<1..914)
                     /locus_tag="PWY89_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL2067420.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="PWY89_002420"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="PWY89_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2067421.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="PWY89_002421"
     CDS             2006..2176
                     /locus_tag="PWY89_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067422.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="PWY89_002422"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="PWY89_002422"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2067423.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="PWY89_002423"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="PWY89_002423"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2067424.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="PWY89_002424"
     CDS             4440..5321
                     /locus_tag="PWY89_002424"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2067425.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="PWY89_002425"
     CDS             5318..6034
                     /locus_tag="PWY89_002425"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2067426.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="PWY89_002426"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="PWY89_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL2067427.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="PWY89_002427"
     CDS             6751..7752
                     /locus_tag="PWY89_002427"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2067428.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="PWY89_002428"
     CDS             7749..9110
                     /locus_tag="PWY89_002428"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2067429.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="PWY89_002429"
     CDS             9070..10221
                     /locus_tag="PWY89_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL2067430.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="PWY89_002430"
     CDS             10196..11365
                     /locus_tag="PWY89_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2067431.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="PWY89_002431"
     CDS             11540..12919
                     /locus_tag="PWY89_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2067432.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="PWY89_002432"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="PWY89_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2924 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DANKBF010000077        13257 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000077 DANKBF010000000
VERSION     DANKBF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(194..2269)
                     /locus_tag="PWY89_002433"
     CDS             complement(194..2269)
                     /locus_tag="PWY89_002433"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2067433.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2271..3188)
                     /gene="glyQ"
                     /locus_tag="PWY89_002434"
     CDS             complement(2271..3188)
                     /gene="glyQ"
                     /locus_tag="PWY89_002434"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2067434.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3574..4392)
                     /gene="recO"
                     /locus_tag="PWY89_002435"
     CDS             complement(3574..4392)
                     /gene="recO"
                     /locus_tag="PWY89_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2067435.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4535..5434)
                     /gene="era"
                     /locus_tag="PWY89_002436"
     CDS             complement(4535..5434)
                     /gene="era"
                     /locus_tag="PWY89_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2067436.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5449..5844)
                     /locus_tag="PWY89_002437"
     CDS             complement(5449..5844)
                     /locus_tag="PWY89_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL2067437.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5828..6304)
                     /gene="ybeY"
                     /locus_tag="PWY89_002438"
     CDS             complement(5828..6304)
                     /gene="ybeY"
                     /locus_tag="PWY89_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2067438.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6333..8525)
                     /locus_tag="PWY89_002439"
     CDS             complement(6333..8525)
                     /locus_tag="PWY89_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL2067439.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8549..9520)
                     /locus_tag="PWY89_002440"
     CDS             complement(8549..9520)
                     /locus_tag="PWY89_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2067440.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10225..11604
                     /gene="brnQ"
                     /locus_tag="PWY89_002441"
     CDS             10225..11604
                     /gene="brnQ"
                     /locus_tag="PWY89_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2067441.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11886..13226
                     /locus_tag="PWY89_002442"
     CDS             11886..13226
                     /locus_tag="PWY89_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2067442.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         3677 a         2832 c         2437 g         4311 t
ORIGIN      
        1 aaataaaaca gtaacaagat tataattaat cgttttcatt tttatttcct cacttttcat
       61 gagcgttttg aaaattcgaa caaagaataa aataacaaaa attattaaaa tagaattaat
      121 aacagctatt tataacaaaa aaaacagaga cacagcacgt ttcgtgcatg ctctctgttt
      181 ttcaaacaaa agactatttt gtgatgattt gatccaagct tgctaatgac aaagccatgg
      241 cagctatttg acttagttgt gttagtcggt tcttacgtac tgctggttca tcagccatga
      301 ccatcgtttg atcaaaataa ttttcgatca gtggacgaag agcgacaagc tggtcgtagt
      361 tttcgtggat cgtttggtca gcaaaacttg ctttcattgc ttctactgct ttgtgtaatt
      421 ctttctctgc gtcgttttcg aacagctcag ggtctacttc tgattgtttt tcaagctgcg
      481 cttttttcgc taagttcacg acacgtgtca aagcttccat tgaaggtttg aagtcttcat
      541 ctgcaagatg ttgtttgaac aattgtgctg ctgcaaatac ttttgtcaga tctttttgtt
      601 ccgcattcaa aacagcatcg atgacatcat gacgaatatt cttcgttgtc aataattggc
      661 gcagacggcc tttgataaag tcgatcactt gttgttgccc ttctgataaa ccaatgccga
      721 aacgatccgt atctttactg ataacatcgc taatcgtttc ttgcagaaca gacaaaggaa
      781 aggcccattt tttcgattcg atgatccgaa cgacaccgta tgcttggcga cgtaaggcat
      841 aaggatcgtt tgacccgctt ggaatcatcc caactgcaaa gaaagaaaag atgctatcta
      901 atttatctgc aatcgctaaa acagaaccta ctgtagactg aggtagctct ccttcactgg
      961 atataggcat gtaatgctca cgaatcgctg tcgctacagc aggtttctcg cctttcagaa
     1021 gagcatattt ttcgcccatg atcccttgta gttcaggaaa ttcccctacc atgttggtta
     1081 ctagatcaaa tttatagatt tcagcagctc gttgcaaatc catcaattct tcctcagata
     1141 gtcctacttg ttcgccaatg atacgggcaa tgacttgtac ccgttgcatt ttctcataga
     1201 tactaccgat tttttcatgg aaagtaacag tttttagttt ttcaacgtat tggtcgattg
     1261 aagctttctt gtcttcatta taaaagaatt ccgcatcttc taaacgagcc gtcaatactt
     1321 tttgattgcc tcgaaccaca tcttccaatt ttacccgatc accattacga gcagaaataa
     1381 aatgaggaag cagcatacct tgatcattcc gtacctcgaa ataacgttga tgttctttca
     1441 tagaagtgac tagtacttct tcaggaacag aaagatattt ttcttcgaag tcgccagaaa
     1501 agacagttgg atattcgact aaattgttga cttcttctag taaatcttcg tctagatcaa
     1561 taacccagtg atttttttct gctaattcgt ttgcttgtgt aacgatcatt tcttgtcttt
     1621 tttgcggaga cgcaatgacg aattgttcat tcaatttttc ttcatattct tttgcatgcg
     1681 caaatgtcac atcttcgcct aagaaacgat gacctcgaga ggctctgcct gttttgacat
     1741 ccaagatttc gaatgggatt acttcgtcat ctaacaatgc cacgatccag tgaatagggc
     1801 ggatatattc aaaatcgtat tctgcccaat gcatcgttac agggaaagtc aaacttttga
     1861 tcacccctgc taagcctgtc aatacttctt ttgcttcctt accatgagtg tatttcgtta
     1921 catagacata ttccacacct tttaattctc taaaagtgat agcgtctgtc gtcagacctt
     1981 gtccgcggac aaatccttcc gctgctttcg tccagtttcc atctgcgtcc aaagcaattt
     2041 ttttggctgg tcctttgact tcttcttcac tgtccgcttg tcgttctgga atatttgtta
     2101 ctttgattgc taaacggcgc ggtgtggaaa atgtttcgat cgtatcataa gtcaattggt
     2161 tttcatctaa aaattttgct gttttttctt ctaattgttt catgcttggt gtgacaacat
     2221 gggcaggcat ttcttccagc ccgatttcaa gtaataagtc ttttgccatc ttattcagcc
     2281 tccttcacgg tttctggatt tgctgtgttt ttattcaaca aagggaatcc taatttttcg
     2341 cgttctgcca caaagatttt ggctacagca cgagccatgt tgcggatgcg agaaagataa
     2401 ccggcacgct ctgttacgga tactgcaccg cgggcgtcta ataagttgaa tgtatggcta
     2461 catttcaaga tataatcata cgccggatga acaagatttt catcgataca gcgtttagct
     2521 tctttttcaa aacgatcaaa gttgcttagt agcaagtctt ggtcgcttac ttcaaaagaa
     2581 tatttggaat gttcatattc tggttgtttg aagatctcgc catatttcac gccatcagcc
     2641 cattctaaat catagacact ttcaacttct tggatataag aagcaagacg ttctaatcca
     2701 taagtgatct cagaagttac tggtttacaa ggcagtccac caacttgctg gaagtacgtg
     2761 aattgtgtga tttccattcc gtcaagccat acttcccaac ccacaccggc acagcccatg
     2821 gatgggttct cccagttgtc ttcaacaaaa cggatgtcgt gttctaatgg atcgattcct
     2881 aaaaggcgca agctttctaa ataaagctct tgaatattct caggtgaagg tttcatcacc
     2941 acttggaatt gatgatgctg atacaaacgg tttgggtttt caccataacg tccatcagct
     3001 ggtcggcgag atggctctac gtaagcagca ttccatggtt ctggtccgat tgcccgtaaa
     3061 aaggtatatg ggctcatcgt acctgctcct ttttctgtgt cataagcttg catcagcatg
     3121 cagccgtttg atgaccaaaa tttttgtaat gttaaaatca tatcttggac ggtcaatttt
     3181 ttactcatct gttttgcccc tttcaatttt aaagcgttgc tgtcaaataa gaatacagga
     3241 aagaaaacga caaaaaatcc ctatgtctga ttacagacat agggacgaat aattcgcggt
     3301 tccaccctac ttccgaataa agattcggca gcttcattta tcgtactcgc aagtgcctct
     3361 tcaaaaatcg tttgggtccg gctcccactc tcccgaactc gctaaaccac gtgtttttta
     3421 ctgatcttgg tcaatgtacg tattcatttt gcagttttct tgtttctata atagatccta
     3481 tcactgaacg atctatttaa tctatggtta ataatagacg gaactccctg tgtttgtcaa
     3541 atggttttga ttttttgttg tatgttttat ttactattct tctttttcag gtgtctcttt
     3601 ttctttttca gacatgtctt tctctgacgt ctttaatacg tgtgcccaag acttcatttg
     3661 attgatgaac tttttgcttt tcaaatggat accgacatat tcatcataca gctggtcgat
     3721 gagttcacgg attgcttgtt tggtttcttc tttcagatgg attccactaa ttttatcata
     3781 agaaatagct gaaaacatcc gaacgaaata aacagctcga ggatctgcat gataacggtg
     3841 atcgtccatc gaccaatgtc tttcgcatat cacaccacca tatttggaag aatagtcaaa
     3901 ttttcctttt gtttccccgc atacgacgca gtgtgtccag attggtgcaa ttccgaagcg
     3961 ttgcagtatt tggacttcaa aaatattcgt gatgatttct gcatcgtctc cttggtccat
     4021 ccgttgcagt gcttgttgcg taaactggaa caaatgcggg tcgtactgat gatcttcgat
     4081 tgccgcatct actagattca ataaatatgt gccatagccg ctcaaaaaga tgtcttcttg
     4141 gatgtgtcga aacgcatgga catctttgac actgcttaaa aatgacaaac cttcacttcg
     4201 aaaattcccg atataaactg cttcagtaaa agggagcaaa gccggagcta agggctgatt
     4261 ttgccgatgg atctctttga tataaaacat cattttgcca gaagattctg taaagatttt
     4321 gactaattta tctttttctt tgtaattacg gctagataaa atcaagcctt ttgattctgc
     4381 aagctggctc atattctccc tcctaaaaaa taaatctgga aaagcagatt ctgccgatcc
     4441 agatcattta tgttgcgatc ttccgcagct tttctcaaca cgcggatttt tattcagttt
     4501 atttctatac ctaatttaca agaagctcag aatatcagta ttcgtctttt cggtagccga
     4561 agtcttgcag atagactttt ttgtctcgcc agtctttttg aactttgacc caaagttcta
     4621 gaaagacttt gtctcctaaa agattttcga tatcttttct tgctttcgtt cctacttgct
     4681 tcagcatttt tccgcctttg ccgataatga tccctttttg gctatctctt tcgacaataa
     4741 tcgtagcctg gatatggatt ttatcattct cgtttcgctt catcgaatca acaacgactg
     4801 cgacagaatg aggcacttcg tctcgtgtta aaaacaagac tttctcacgg atcaattcag
     4861 aaacgatgaa gtattcagga tgatcagtca cttgatcatc tggaaaatat tgtgggccct
     4921 ctggcatttc actgactaat acatccatca aacgttcgac gttgtttcct tctgtagcag
     4981 agattggaat cacttgtgca aaaggcatct gcgttgaata atcttcgatg attcccaaca
     5041 actcgtctgg atggattttg tcgattttat tgataatcag ataaacagga gaatgcacgt
     5101 tttttagacg ttcaataata aaatcgtctc cccgaccgcg tttttgatca gcactaatca
     5161 taaataagac cgcgtccact tctttcaacg cactgtaagc tgcttctacc ataaaatctc
     5221 ctaaacgatg ctttggttta tggattcctg gtgtatcaat aaaaatcaac tgagcttctg
     5281 gtgtagtgta tactccttgg attttatttc tagttgtctg tgccttgtca ctcatgatgg
     5341 caattttttg acccacgatt cgattcaata atgtggattt ccctacattt gggcggccga
     5401 cgattgcgac aaaccctgat ttatgttctg gcatatatgt tctccttttt aattaaacaa
     5461 caaatgatat attttaggga caaataaaat caacccaacg atgacggtaa aacaagcggt
     5521 cactaaaact gctccagctg ccatatcttt gatttttttc ccaatcggat gaaaatgaaa
     5581 atccgtaaac atgtccacga cattttcaaa aacagtattg atgatctcta ctatccatac
     5641 gagaaaaaca gctaaaagca gccataacca ttctatccga tcaagttgga aaatcacgcc
     5701 cataaacaaa gcgaggattc cgaacgcaac atgtttcctc atgttgcgtt cttcctcaaa
     5761 aacagttttg actccttgga cggcaaattc cacggacatc aggaaatgtt tgtttttctc
     5821 tacgttttta tcttttaagt ccataggcat ctaagatctc tttctgtaaa ccaaacattt
     5881 ctttctcatc ctctggtgtc atatggtcat acccgttgat atgcaagaat ccatgaaccg
     5941 ctaaaaagcc aagttcccga tcatacgaat ggccgtaagc ttctgcttgc tctgcagcac
     6001 gttcagtaga gatcatgatg tctcccagta cacgaggcaa tggattctct ccatcttcaa
     6061 aaatcactgg gatttcgtct tcgccctctt cttccaaggc aaaactgatg acgtctgtcg
     6121 gtgcatcttt tccacggtaa tcacgattga ttacttggat tgctgcattg tccatgaaag
     6181 taacagacat ttctgtttct tctggcaact ctaaatatga tgctgcaaat tgcagcaagt
     6241 cgtcgatttc tttcattttc tcttctgaga cttggtttgt ttcatccatg aaggtaatat
     6301 ccattataca tcgcttcttt ctattcttct tgctattctt cttgttcttc ttttatcttc
     6361 tcaacttccg atttcatttt aggatatttg atccgagaat ggaaggtact gcttaaaccg
     6421 ctgatcaatg atttttctac tttacggatt tcttttaacg tcaatccact gtcgtccaat
     6481 tgtccatcgg aaatacgctc ttcaataaga ttatggacaa aactttcgat tttttcgctc
     6541 gttggatggt ccattgcccg tactgccgct tcacaactgt cagctatatt gacgattcct
     6601 gcttcacggc tttgaggttt tggtccagga taacggaatt gctcttccgt aacttccggg
     6661 ttgcgttcct ttgccttaac gtagaaataa cgcatcagcg tggttccatg atgctgtcta
     6721 caaatatcga tcaccatttg cggcatatta taatcttcca atattttggc accatctgtc
     6781 acatgtccaa agataatatg tttactgtct tctggcaata agaaattgtg cggattttcc
     6841 gctcctgaag gcaagttctc aacgaaaaag ctggcgtgtt tgatcttgcc gatatcatga
     6901 taatagcaag ctactcgagt tagtaaagaa cgtccaccga tttctgctac agcattagca
     6961 cttagattgg ctaccatcat gctatggtga tacgtacctg gtgcttcttc cagcaactgt
     7021 ttcaataaag gatgattggg attgctgagt tcatttaata cgatcacact atcatcattg
     7081 accatcaatt caatataggg gtgcagacct atcgtcagca agaatgaaaa cagtgcacct
     7141 gtgaatccgc agactagcgt taaccaagtc gttgcatccc caaagctcat tccttggtag
     7201 ataatcaaaa tgacatccat catcacagga aaaataatga cccacatagc agcggataac
     7261 ccttgttctg agatccgttt gcgtctgacg atcgttgcca tgagaccaga aaatacatag
     7321 gacatcaaaa taactgtcag aaagttcgtg ccaatcgaat cataaaaaat gaaaagtgcg
     7381 gatactgctt gaaaaatacc agccatgatc cctgctcttc gattcaagaa aaagcttaaa
     7441 acaagtggag caaaagccgc ggggtaaaac agcggaatat aagcagcttg ttctgtttgg
     7501 aagagttgga aaaatttcat tagtacgacg cttactgaca ttgccgtcac gtagaaaagt
     7561 acgtattttg ttctttctcc tgcattttgg aattgaagtg aattataaat cagtacaccg
     7621 atttgaagca aaacagctaa aaccatggca atgagtggga aaatagaagt cgtttgattc
     7681 gtcaatccta gtaattccaa ttttttcatc gctgaagcat caatttgatt tccttcgcga
     7741 acaataattt ctccttggaa gatcatcact ggttgtacag cttctcgtgc agattgtttt
     7801 aattcttctg tctttttctc attcaataca tcattaacta cgatgccttg atccactaag
     7861 tagcgaatca tttgttgctg ctctgttgtg acattcaaat attgaatctg atcttctgct
     7921 tcttgtttaa aagcttccaa ttcgctttca cgaacttgtt tactcatctg ttcgtcaatg
     7981 agcttcaaac tttcatccct aactgtgtga atttccgttt cagtcatttc aaatatcgtc
     8041 gaataaaaat tgttcggcag tttttggtaa aacgcaacgt tttctgcatt cacgttttca
     8101 aaatttttct tcagtgtagc aatccgctct tctgtagtag gtttcgggat attttcatct
     8161 tccttcgcct tatctacctt gccattataa gatttgttga tggtatcatt cgtcttatcg
     8221 atcaattcga ataattgctt gatccgattg ttctggtcat ctgccagatc tttttgataa
     8281 gtatattctg gagtcactgc ttctgctgcc agttttcttc tttgttcggt ttcttccgtg
     8341 ttctcaatcg ttttattcgc acggatactc tcttctgcta cttggccttc tctgaaatca
     8401 atggcctttt gtttgacact actaaatgta acgccgcaga caatgataaa aaacaagaac
     8461 agaaggaaag gcaatgctgc ctttccgagt ttttcttgta gtttctgcag tgttttattc
     8521 aacatgtgct tatcctccta agcaaagctt attcttgatg aagatccgcc ttttcataag
     8581 ctttgatgat ttcggcaact acagggtgcc gaacaacgtc acttgcttca aaattaacaa
     8641 aatcgatttt tttgatttgc tttaatgttc gttctgcatg aacaagacca cttgtcgtcc
     8701 cacgaggcaa gtctatctga cttgtatccc cattgacaat catttttgat tgataaccga
     8761 gacgggtcaa gaacattttc atctgagcga ctgtcgtgtt ttgtgcttcg tccaagataa
     8821 cgaatgcatc atccaatgtc cgcccccgca tgtaagctaa aggtgcgatt tcgatcacgc
     8881 ctcgttccat caatcggttc gtatgatcca atccaaaaat ttggtataaa gcatcataaa
     8941 caggccgcag ataaggatcc actttttctt tcagatcccc gggtagaaag cctaggtttt
     9001 cccctgcctc cacagcgggc cgcgtcaaaa tgattttttg cacttcgcct ttttttaggg
     9061 cagcaattgc taatactacg gcaagaaacg ttttccctgt tccggctggc cctacaccaa
     9121 atacgatgtc atgtttggct accgattcaa cgtatttctt ttgaccgctg ttttttaccc
     9181 gtataggttt tccattacgg tctttgacga tctcttcctc atacatctca acaaaataat
     9241 ctaatgtacc tttttgcccc attttcaatg ccgtcacaac atctggcgta cttacgtgga
     9301 ttcctcgttt gatcaattcc tgtaatgtcc gaatgactga ggcagccaag gaaatctggg
     9361 attgctcacc aatgatctga atgatttctc cacgtgtatg gatggttgtt tccgttgttt
     9421 cctcgatcag ttttatatgc ttgtcgtgtg cacctaaaag catgctgaca tcatcagcag
     9481 ctgtcaatct aatctctaaa gaactagact cttccgtcaa aaatagacat cccctttttt
     9541 tcgattcatt ctttcctatt ttaccatact cagaagaaac tatatagcaa ataagaaaag
     9601 gttgtaaaag agtcgcttaa ccatacgtac caagaaacgt taaaaaatag ccgtttccat
     9661 tcgctaaagg tctcgttttg ataaaaagaa gctgagaatg taagttatat gaaagtggag
     9721 aacaaaatat ttcgtttcat tcaacaagta ttctctaggt atttttcctg tttcaaaaaa
     9781 agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa aacaggtgta ttattacgtc
     9841 aacaaataaa catacagcaa agcgtcgagg aaaacagtag gctattcaac gatttattcc
     9901 cagtgagtca tgtaagtgag aaatgacaat aaatcgaata gacgaacatc atttccgagt
     9961 caatgtcgaa aacgtagatc attgtggctg cctccattat caggctagcg gataacttta
    10021 tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt accgttctag
    10081 aatctagagc ccttttccca agtggaaaag ggcttttttt tattttaagg tggtggtttt
    10141 catggaatct gatattagtg gcattactaa ttgctgcaac gtttatatcg tcaatcaaaa
    10201 tgataattat tggaggaaaa atttatggaa gaaacaaaaa cttattcatg gaaacaacta
    10261 ttgatggtca gttcacttat cttcgggatg ttcttcgggt caggtaatct aatctttcct
    10321 gtccacttag gtcaaatggc tggcgcaaac tgggtcagtg ctggcgtagg atttgctatc
    10381 tcaggtgctc ttttcccatt actagctatc ttagctgtag tagtgacaaa aagtgatggt
    10441 ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg ccttgttctt aatccttgtt
    10501 catttgacga tcggaccatt tttcggtaca ccacggacag ctgctactgc ttttgaaatg
    10561 gctgctaaac catttttgcc tgaaaaatat aattcattag gtatgttttt attctctgct
    10621 cttttctttt tacttgctta tttccttact gtccatccaa cacggttggt caaatacgtc
    10681 ggaaagtatc taaactcctt gtttcttgca ttattagcaa tcatttttgt cgtttcttta
    10741 ttccatccaa tgggaaatct aaatcaatct gctgcagata cttatcaaac aaacgcttta
    10801 acaaatggtg tcctagaagg ttataacacg gttgatgcag tagctttgct tgccttaagt
    10861 gtcacttttg tccatgcagt ccgtgacctt ggtttcaaag ataaaaaagt cacagaatta
    10921 acggcaaaag ctggtagcct gagcgttgcg ctagaagtca tgatctattt tggacttgtt
    10981 cttctcggtg cgatgagttt gaataagatg tctttatcag aaaatggcgg aactgcactt
    11041 tctcagatca ctaattttta tttaggtaac ttcggcgcta gcttcttagg agtgatggtc
    11101 acattaggtg tcttcactac cgctatggga ctcgttgttt cttttgcgca agatttccat
    11161 aaactgttcc ctaaagtcag ttatatgaca tggctgcggt taacgacttt cgtctctttt
    11221 gtcgttgcaa atgctggttt agacaatatc atccaatggt ctttaccagt attgatgctt
    11281 ttgtatccat tgtcgcttgc gttgatcttg ctttccttaa cagcaaaatt tttccaaaag
    11341 acaccatttg tctatcaagt aacgatgttg tttgctgctg ttcctgccgt attggatatg
    11401 ctggcaaatt cgcctgcttt agtgagtcaa caaagagttg tagcaagcat gctcgaattt
    11461 tatcatcatc acgttccttt tgctgcatta ggacttggct ggatggtccc aacgttactt
    11521 ggttacgctg gcagcctact gttttattac gcctatcgcc tatcagggta taaacaagaa
    11581 gcaaatgaac taccagaaga ataaccaaat caaacacaat ttaacataag aaatattaaa
    11641 caggaaaaaa ccagctagat gaaagaatcc tattctttcg tctagctatt ttttagttct
    11701 cttttttacg cgcacaaaaa aactgcggac aatgattgtc cgcagtttaa atgggctcta
    11761 taatatctag tgttggtgac gccttctgtt gccaatactt tttctttttt ctaataaacg
    11821 caaacagaca ccttgaaatc ctttagaata aagtcgacga aaaccattca aaggagtgtt
    11881 tcaagatgtc ctattcagag tttaccaaag agttactaga tattctagac ctaaatctta
    11941 tctttcatga agatgccttt cgcaaagagc gaatcaatga cgaaacatgt tttgtttttg
    12001 atggcacatt gacctatcaa ccagaggaat gcttccattg tcactatcaa aataagcaaa
    12061 cgatcattaa atggggctgg aaaaaagtat ccatcttatt gaatgatgtc agtaactaca
    12121 agaccattct gcggattaac aaacagcgct tcaaatgtaa acactgtgga aagacttttt
    12181 tagcagaaga ttcagttagt gatcgtcgct gctccattgc tcgcagagtc aaacaagcca
    12241 ttcttgagct attaagcgaa cctctttcta tgtctttgat cgcacgtatg aagcacattt
    12301 ctccgaccac tgtcattcga atacttcgca gccttcggcc taaaaccgtt tctttgaacc
    12361 agccgttacc agaagtggtg tgtttcgatg aattcaaatc ggtgaaaaac gtctcgggag
    12421 ccatgagctt tgtcatgatg gacggcaaga cccatcaact gattgatatt gtggaaaatc
    12481 ggcaactaaa tcctttacgc gactatttct tgcgctaccc tagaaaagtc cgtgaacaag
    12541 tacgtctagt ggtttcagat ttctatacac cctatcgcac attggtgaaa gagtgctttc
    12601 cgaatgcccg aatcgtcgcc gatcgctttc atattagcca acacatcggt cgggccttta
    12661 ccaaccaccg aattcaagta atgaagacct ttaaaaaaag ggatcgtcgg tcgaagcact
    12721 taaagaaata ctggagacta cttcaaaaga atgcgtggga attgaaaggt cagcatcgct
    12781 actggcgtcc gtcatttcga gatcatctga cagaagcaga gatagtcgat cggctattat
    12841 cttatgatga ctccttaaag cgaggctacg aagtctacca ggattttctt tccgctatca
    12901 gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga agactacaag gagttgccag
    12961 agcactacca accagtgttc acgaccttca agaaatatcg aacagaaatc aaacgagcgt
    13021 tgcgagttcc ctattccaac ggccctatag aatgtttgaa caaccatatc aaagtactaa
    13081 aacgaattgc ctatggcttt agaaactttc aaaactacca agaacgaatc tttttatatc
    13141 gaggaaaata ttttaagaaa actaagaaca ctacccaact gactaaagct agaacaacaa
    13201 cacggctaga caagatagct gtctagccgt gtgagtatct cgattattct attgaat
//
LOCUS       DANKBF010000078        13148 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000078 DANKBF010000000
VERSION     DANKBF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            237..2402
                     /gene="acm"
                     /locus_tag="PWY89_002443"
     CDS             237..2402
                     /gene="acm"
                     /locus_tag="PWY89_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL2067443.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2674..3159)
                     /locus_tag="PWY89_002444"
     CDS             complement(2674..3159)
                     /locus_tag="PWY89_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2067444.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3249..3594
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3639..4280
                     /locus_tag="PWY89_002445"
     CDS             3639..4280
                     /locus_tag="PWY89_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2067445.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4299..4934
                     /locus_tag="PWY89_002446"
     CDS             4299..4934
                     /locus_tag="PWY89_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2067446.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4952..5767
                     /locus_tag="PWY89_002447"
     CDS             4952..5767
                     /locus_tag="PWY89_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2067447.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6086..7522
                     /locus_tag="PWY89_002448"
     CDS             6086..7522
                     /locus_tag="PWY89_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2067448.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7711..10158
                     /locus_tag="PWY89_002449"
     CDS             7711..10158
                     /locus_tag="PWY89_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2067449.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10388..11455
                     /gene="rlmN"
                     /locus_tag="PWY89_002450"
     CDS             10388..11455
                     /gene="rlmN"
                     /locus_tag="PWY89_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2067450.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11648..12751)
                     /locus_tag="PWY89_002451"
     CDS             complement(11648..12751)
                     /locus_tag="PWY89_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2067451.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4510 a         2184 c         2770 g         3684 t
ORIGIN      
        1 aaaaaatgaa agcaataagt aaaataaaat gaatatatgc gaaaaaataa tttacattag
       61 gaaaaacctt gtaaaaacag ttttttggca tatttatttt ttataaatat ttgtaaaata
      121 actatcaatt ttttagttaa aagattggaa atgagtgggg gaggaattga agaaaggatt
      181 tttgagagat gatatagtag gttttatttt tagttaaaat aggggagata gaaaaaatga
      241 aaaagtgtag aaatattatt ttatcaatgt tatttattat tactaatata acatcactga
      301 ttcctgttca tgtctatgcg gatgcaggca gagatatcag cagtaatgtc acttcgttga
      361 ccgtggaccc aacgaatatt acagatggag gaaacataaa agtaaaattc tcgtttgacg
      421 aaaaaaaaca aaatatccaa ccaggtgatt atctttggat caattggcca agtgaaggaa
      481 atattcgcgg tgaaggattc caaaaagaaa tcccattaat gattgaaaat aaaaatgttg
      541 gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt taatgaaaat attaaaaacc
      601 tagactcagt agaaggttgg gggcaatttg agatacaggc cagaaacgta accgataccg
      661 gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga taaaacagct actgtaaatg
      721 tgacaaagcc agcttctggt tcttcttcta gcgtttttta ttataaaaca ggagacatgc
      781 tacctgaaga tactaagcac attcgatggt ttttgaatat taacaataac ggcacttatg
      841 ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg acaaagatta gatccaagta
      901 cgtttgaaat taatcaaata cacttaggtg aacagaaggt ttatagagga gaagagggaa
      961 tccaacaatt tttacaagat ttccccagcg ctacatttaa tttttcagta actgataatt
     1021 acattgaaat aactatacca aaaaactttg tcaatcttag gaaaataatg gtgagttata
     1081 agacgatcat agaaaaccca gaacaaataa attttgagaa tcattcggaa gcttggttta
     1141 aagaatttaa taaacctgct gttgatggag aaagtttcaa tcatacagtt aaaaatattt
     1201 cagcttctgg tggagtcaac ggtacagtta gaggcgaatt gaagattttc aaatatatca
     1261 atgacacaga gattggtatc ccaaatgtga ctttcgaatt aagacgagca gatgaacagc
     1321 ctattcaagg acagtctagt atcctgttga cttcgaatga acagggagaa gctagtatca
     1381 aaggcctgca agtaggagac tatgttgtaa aagaaaaaga agcacccaat tggattgatt
     1441 ttgatccact aagttcaaat gaactcaaat tctccataaa cgaaaatgat acagagggag
     1501 tcagtttacc tatttataac aagaaaaagg ttacgaacat cacagcaact aaaatttgga
     1561 acggtggaac cactccaaga ccgagtattt attttaaatt attcagagca agtacaaaca
     1621 attgggaacc agttcctgat gcagaaacaa aacgattaga caatggaata acttcagtga
     1681 cttgggaaga tatccaacag tatgatgata gtggaaacga atatacgttt aaagtgcaag
     1741 aagtagatca aaacggaaat gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc
     1801 tagtcgttac aaatgagaat aaagaagtca tttcagtctc aggacaaaaa acatgggaag
     1861 acaatgagaa ccaagacggc aaacgaccga cgacgatcac agtaaatcta ttagccgacg
     1921 gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa
     1981 atctgccaaa atacagagac gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc
     2041 ccgaatacag tacgacgatt gacgggtata acatcaaaaa cagttataca ccggaaaaaa
     2101 caaatatatc tataccaggt agccatataa caccgtggaa aaattttcca gagaagacag
     2161 agagagagaa aattagaaat ctattctctc aaaaattgca aatatttgct catacaggag
     2221 aatccagcga acaaagaagc aattatatta gtaagagagc tgtacctaaa cagataacaa
     2281 ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca
     2341 ttggtttatt gatagtaact atctgtgctg ggatattctt acaaaaatat aaaaaagttt
     2401 agttagaata taatattttt tgttaaatat gactgaaatc aaaagaataa aattccgatt
     2461 tataatcgaa aaaagaagca tttttatata tgcttctttt ttcgtagaat caataaatat
     2521 ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata ctagttgtta
     2581 cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa gaaaaagacg
     2641 cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa ctggcagatg
     2701 ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt
     2761 gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga tcacgacttg
     2821 gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg ctggaacgcc
     2881 taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt ttaattgttc
     2941 agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat agatttgttt
     3001 tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg aaaaagggac
     3061 atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac ccagtttttt
     3121 tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc ctcctttttt
     3181 tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa
     3241 agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag agagtctctg
     3301 gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat tttctgaagc
     3361 gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga tgaccagata
     3421 aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat taacgtcctt
     3481 tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg tgtgaggttt
     3541 tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct cttggcattt
     3601 gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg attgaagtta
     3661 tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt accttgattg
     3721 gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt aagccgctac
     3781 gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg ttgcaactga
     3841 tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac
     3901 tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt ggagggatac
     3961 aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa
     4021 cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct gtagggaatg
     4081 aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta ggtgacgtaa
     4141 tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg ctaggcgtgg
     4201 caatcatcta tcttacattg actggtattc taacaatcat aatgaaacaa gttgaaggaa
     4261 aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg aagaaagtga
     4321 acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt ccagatggga
     4381 aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta cgtatcttgg
     4441 ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg ttcaacccat
     4501 tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc cagctatttc
     4561 cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg aaacagacaa
     4621 aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt agtgatttat
     4681 tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagct attgccagag
     4741 cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct ttagatcctg
     4801 aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg gtaacccaaa
     4861 ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt
     4921 ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc tattcgcttt
     4981 gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag cagataattg
     5041 ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat ttgttcctat
     5101 gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag ccaaagcagt
     5161 ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa tgaaggaatt
     5221 tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa cgccggcaag
     5281 agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc tgatcacacc
     5341 taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag gtgcgcaaaa
     5401 tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag agatagtcaa
     5461 aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc tgaaaagcag
     5521 acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga aacaaagaaa
     5581 cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt ttgcagttgg
     5641 tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag atcaactaga
     5701 gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg ttacgccgag
     5761 aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac
     5821 agtgttctcc tgccaactcc ttgtgactcg gagctgaatg gcaggctcac aacctcttgg
     5881 taaacggtgt tcaaaagcca gtttttcggt attaagccaa atttatgcaa aaatgtagaa
     5941 agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc acaacctcct
     6001 ttacataatt ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg
     6061 aaggttatag gaaaggaaga gatgaatgga acacaaagaa cttacctcat ttccaaaaga
     6121 ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg
     6181 gaaaggagaa tcagtctggg atcgatttgt acggatacct ggaaagacat ttaaaggaac
     6241 gaacggtgat ttggcagtag accactatca tcgttataaa gaagatattg ctttaatgaa
     6301 agaacaaggt ctgaaagctt accgcttctc tgtagcttgg acacggattt ttcctaatgg
     6361 acgaggagaa gtcaatcaag ctgggctcgc tttttatgag agattgatcg acgaattgat
     6421 tgaaaacgat attgaaccta tgttgacttt atatcattgg gatcttccac aagcattgca
     6481 agatgaatat aatggttggg aatcacgtca gatcattgaa gatttcactc attatgcaga
     6541 aacattattt gaggcattcc gcgggaaagt ccattactgg attagtttga atgaacaaaa
     6601 tatcttcact tcattaggtt atttgttagc agcacatccg ccaggtgtga cagacccgaa
     6661 acgaatgtat gaagttaacc atattgctaa tttagcaaat gcatctgtca tcaataaatt
     6721 tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc caaattatcc
     6781 aattgacagt aatcctgaaa atgttctggc tgctgaaaat gcagaagacc tgatggccaa
     6841 ttattggatg gatgtctata tgtggggaaa atatccgatt gctgccatga ggttcttaga
     6901 agaaaaagga tgggcaccaa cgattgaagc tggcgatgca gaattgctag aatctgctaa
     6961 accagatttt cttgggatca attactatca aacggcgacg aatgcattca atccattaaa
     7021 cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg
     7081 agttcctgga atgtataaaa aagttgagaa tccatttgtc gaacgaacaa attgggactg
     7141 ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat
     7201 gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca
     7261 aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc
     7321 aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag
     7381 ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca
     7441 agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga
     7501 agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg
     7561 ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa
     7621 aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat
     7681 aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa
     7741 aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt
     7801 ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta
     7861 ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt
     7921 cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt
     7981 aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat
     8041 gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat
     8101 ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt
     8161 ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca
     8221 gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg
     8281 ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc
     8341 aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa
     8401 gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag
     8461 aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat
     8521 acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct
     8581 gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt
     8641 tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag
     8701 gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat
     8761 caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa
     8821 agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc
     8881 gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc
     8941 gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg
     9001 attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa
     9061 gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga
     9121 cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt
     9181 agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc
     9241 catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc
     9301 atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa
     9361 aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat
     9421 atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca
     9481 accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc
     9541 aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat
     9601 atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat
     9661 gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat
     9721 tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag
     9781 aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt
     9841 caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa
     9901 gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca
     9961 cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt
    10021 tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca
    10081 gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg
    10141 gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa
    10201 agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt
    10261 atgatgtctt gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag
    10321 tattgcatta aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga
    10381 aaatactttg gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt
    10441 tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa
    10501 acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc
    10561 ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac
    10621 agtaaaatat ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca
    10681 agaatatggc ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt
    10741 ttgtgcaagc gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca
    10801 gatcatgatg gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt
    10861 cgttgtaatg gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca
    10921 tatcatcaat gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag
    10981 tggattagca cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat
    11041 ttccttgcac gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt
    11101 cccaattgag aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat
    11161 cacgtttgaa tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt
    11221 agcagatcta ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc
    11281 agtcagtgaa catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga
    11341 tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga
    11401 tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta
    11461 tcctactcgt tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca
    11521 acttaaataa gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa
    11581 ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag
    11641 caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt
    11701 ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa
    11761 tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat
    11821 tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa
    11881 acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc
    11941 cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca
    12001 tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat
    12061 ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt
    12121 gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat
    12181 ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag
    12241 catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa
    12301 tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt
    12361 tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga
    12421 agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac
    12481 cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat
    12541 ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca
    12601 aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta
    12661 cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg
    12721 tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg
    12781 ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc
    12841 taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc
    12901 ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta
    12961 gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat
    13021 tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga
    13081 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
    13141 ttcctatt
//
LOCUS       DANKBF010000079        12747 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000079 DANKBF010000000
VERSION     DANKBF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="PWY89_002452"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="PWY89_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2067452.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="PWY89_002453"
     CDS             complement(323..892)
                     /locus_tag="PWY89_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL2067453.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="PWY89_002454"
     CDS             1078..1980
                     /locus_tag="PWY89_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2067454.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="PWY89_002455"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="PWY89_002455"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2067455.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="PWY89_002456"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="PWY89_002456"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2067456.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="PWY89_002457"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="PWY89_002457"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2067457.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="PWY89_002458"
     CDS             8554..8739
                     /locus_tag="PWY89_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL2067458.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="PWY89_002459"
     CDS             complement(8801..9997)
                     /locus_tag="PWY89_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2067459.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="PWY89_002460"
     CDS             complement(10167..11603)
                     /locus_tag="PWY89_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2067460.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="PWY89_002461"
     CDS             11896..12705
                     /locus_tag="PWY89_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2067461.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DANKBF010000080        12600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000080 DANKBF010000000
VERSION     DANKBF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(324..893)
                     /locus_tag="PWY89_002462"
     CDS             complement(324..893)
                     /locus_tag="PWY89_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2067462.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="PWY89_002463"
     CDS             complement(967..2256)
                     /locus_tag="PWY89_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067463.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="PWY89_002464"
     CDS             2610..3458
                     /locus_tag="PWY89_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL2067464.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="PWY89_002465"
     CDS             3472..4545
                     /locus_tag="PWY89_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL2067465.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="PWY89_002466"
     CDS             4535..6706
                     /locus_tag="PWY89_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDL2067466.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="PWY89_002467"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="PWY89_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL2067467.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="PWY89_002468"
     CDS             complement(9117..11303)
                     /locus_tag="PWY89_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2067468.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="PWY89_002469"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="PWY89_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL2067469.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="PWY89_002470"
     CDS             complement(11525..11965)
                     /locus_tag="PWY89_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2067470.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="PWY89_002471"
     CDS             complement(12039..12512)
                     /locus_tag="PWY89_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HDL2067471.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
BASE COUNT         3537 a         2652 c         2235 g         4176 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
//
LOCUS       DANKBF010000081        12571 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000081 DANKBF010000000
VERSION     DANKBF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(132..2477)
                     /locus_tag="PWY89_002472"
     CDS             complement(132..2477)
                     /locus_tag="PWY89_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2067472.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="PWY89_002473"
     CDS             2756..3658
                     /locus_tag="PWY89_002473"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2067473.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="PWY89_002474"
     CDS             3869..5644
                     /locus_tag="PWY89_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2067474.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="PWY89_002475"
     CDS             complement(5710..7296)
                     /locus_tag="PWY89_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2067475.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="PWY89_002476"
     CDS             7547..7771
                     /locus_tag="PWY89_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL2067476.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="PWY89_002477"
     CDS             7913..9664
                     /locus_tag="PWY89_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2067477.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="PWY89_002478"
     CDS             9664..11448
                     /locus_tag="PWY89_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2067478.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12571
                     /locus_tag="PWY89_002479"
                     /pseudo
     CDS             11783..12571
                     /locus_tag="PWY89_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
BASE COUNT         3877 a         2329 c         2567 g         3798 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca c
//
LOCUS       DANKBF010000082        12335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000082 DANKBF010000000
VERSION     DANKBF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..154
                     /locus_tag="PWY89_002480"
     CDS             <1..154
                     /locus_tag="PWY89_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2067479.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /gene="istA"
                     /locus_tag="PWY89_002481"
                     /pseudo
     CDS             complement(235..1218)
                     /gene="istA"
                     /locus_tag="PWY89_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="PWY89_002482"
     CDS             complement(1613..2455)
                     /locus_tag="PWY89_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2067480.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="PWY89_002483"
     CDS             2780..3196
                     /locus_tag="PWY89_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2067481.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="PWY89_002484"
     CDS             3193..3663
                     /locus_tag="PWY89_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2067482.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="PWY89_002485"
     CDS             3677..4450
                     /locus_tag="PWY89_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2067483.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="PWY89_002486"
     CDS             4464..5276
                     /locus_tag="PWY89_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2067484.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="PWY89_002487"
     CDS             5330..6295
                     /locus_tag="PWY89_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2067485.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="PWY89_002488"
     CDS             6431..9154
                     /locus_tag="PWY89_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2067486.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="PWY89_002489"
     CDS             9167..9799
                     /locus_tag="PWY89_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2067487.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10004..10231
                     /locus_tag="PWY89_002490"
     CDS             10004..10231
                     /locus_tag="PWY89_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDL2067488.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10300..10566
                     /locus_tag="PWY89_002491"
     CDS             10300..10566
                     /locus_tag="PWY89_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2067489.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="PWY89_002492"
     CDS             10556..10825
                     /locus_tag="PWY89_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2067490.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="PWY89_002493"
     CDS             complement(11004..12305)
                     /locus_tag="PWY89_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2067491.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4029 a         1998 c         2422 g         3886 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtg
//
LOCUS       DANKBF010000083        11771 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000083 DANKBF010000000
VERSION     DANKBF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(49..495)
                     /locus_tag="PWY89_002494"
     CDS             complement(49..495)
                     /locus_tag="PWY89_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL2067492.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(522..698)
                     /locus_tag="PWY89_002495"
     CDS             complement(522..698)
                     /locus_tag="PWY89_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2067493.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(860..1288)
                     /locus_tag="PWY89_002496"
     CDS             complement(860..1288)
                     /locus_tag="PWY89_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2067494.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1380..2240
                     /locus_tag="PWY89_002497"
     CDS             1380..2240
                     /locus_tag="PWY89_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2067495.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(2334..2684)
                     /locus_tag="PWY89_002498"
     CDS             complement(2334..2684)
                     /locus_tag="PWY89_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2067496.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2700..4427)
                     /locus_tag="PWY89_002499"
     CDS             complement(2700..4427)
                     /locus_tag="PWY89_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2067497.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="PWY89_002500"
     CDS             complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="PWY89_002500"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2067498.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5711..7147)
                     /locus_tag="PWY89_002501"
     CDS             complement(5711..7147)
                     /locus_tag="PWY89_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2067499.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7144..8547)
                     /locus_tag="PWY89_002502"
     CDS             complement(7144..8547)
                     /locus_tag="PWY89_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2067500.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            8777..9679
                     /locus_tag="PWY89_002503"
     CDS             8777..9679
                     /locus_tag="PWY89_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2067501.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(9720..11606)
                     /locus_tag="PWY89_002504"
     CDS             complement(9720..11606)
                     /locus_tag="PWY89_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2067502.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
BASE COUNT         3293 a         2619 c         2027 g         3832 t
ORIGIN      
        1 gaatttcttc accaacactc attgacgaag agcctttaaa tgataaaatt aagattgtaa
       61 tagttctttc acaatggcat ttacttggtt tccatctgct tttcctttga ctttaggcat
      121 gatcgcaccc atgactttgc caaattcttt cattgatgaa gcaccagttt gtgtgattgc
      181 ttcttgaaca agttgtcgaa tttcttcgtc ggaaagttgt tccggcatgt atttttcgac
      241 tatcacaatt tcacttttta ctttttcagc taaatcgtca cgtcctgctt cttcaaactc
      301 atgcaaagaa tcacgtcgct gtttcatttc gcgagacaac accgttaatt cttcttcttc
      361 tgtcaagtcg tgaccttctt taatctgctc gttttgtata gatgacttga tcattcggat
      421 gacttgtaaa gattccttat ctttcgcttt catcgctgtt ttcatgtcat cgtttaatct
      481 acttagtagt gacacactac caactccaat tcatcacaga actagaattt tttgcgtttt
      541 ctagctgctt cagatttttt cttgcgtctt acacttggtt tttcgtagaa ttcacgttta
      601 cgagattctt gtaaagtacc cgcttttgac acggaacgtt tgaagcgacg aagagcatcg
      661 tcaagtgatt cgtttttgcg aacgactgtt tttgacatgt taattccctc cctccgagct
      721 taaaaagtaa ccgtaattgt tattgaaata tatctcaaaa aacaagactg tcctgtaaat
      781 attatatcta atgctgtttt gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa
      841 tgttatttta tttaaatcct tattgacaat tttcacatct tccgaaaatc tcgaatctat
      901 gcccttcgat cgtacatcca ccgagctgtt cttcgaaata attcattgga cacatatgga
      961 tttctttcgt tgttccgcac accgtacaga taaagtgatg gtgatggccg atttcccctt
     1021 gacagcaatg aaaacgaaac ttcatctctc catttaaatc ggtttcttcc agtaaatcga
     1081 tttctgcaaa atcatggaga tttcgataaa tcgtatcgta acttactcct ttgaattcct
     1141 gattcatata gtcaaaaact tctttagcgg atacgtagcg attgcgcttc actaaataag
     1201 ttaacaatgc ttctcttttt ttagtatatt taaaaccgtt atttttcaat gtttctagtg
     1261 attgttcaat caatgtctgt tttttcatga cttattcctc caaatcaaaa tgattacgaa
     1321 gtacacattc tagagtatgg tataataaac ataaaaaaaa tgcaagtgag gttttatcca
     1381 tgtcttccga ttctacagaa atcattactt tttttgtcat ctctgactct gctggagaaa
     1441 ctgcgacaaa attagcgcaa gccactatgg cccaatatcc taccgttgag ttcaatttat
     1501 tccgcagaac atttgtgaca gatgagaaaa cattgatgca ggcactgcaa gatgcactaa
     1561 aagaaaaagc ccttgtccta cacacattga tcaatcaaga attgatcgag atgacgcgtt
     1621 ctttttgtga agaacatcaa ttatttagtt ttgatgtgat gactccgccg atatcagaaa
     1681 tcgagcgctt aactggtatc aagcccattc ggcaacctgg cgcccttcat ttattgaatg
     1741 agaactattt caaacggatc aaagcaatgg aatttgctgt aaaatacgat gacgggaaag
     1801 atcctcgtgg gtttctagaa gcagatgtcg ttttgctagg cgtatccaga acttccaaaa
     1861 cgcctttgag cttgttttta gctaacaaaa atttaaaggt tgccaatcta cctttgatcc
     1921 cacaagcaca tattcccaaa cagctttggg agatcgatcc taaaaaaatc gtgggactga
     1981 cgaataaccc agatatttta aacaatatcc gtaaagaacg gatgcgctct tacggattaa
     2041 atcctgatac tgcttattcc gatatcgaaa aaatacgagc tgaactcgat tttgcgaatg
     2101 atctttatga aaaattgggc tgcgtagtaa tcaatgtcgc ttctttatct atcgaagaaa
     2161 cagcttcact tattttgaat gctttagacc tagaagatca tagttattat ggaactgaaa
     2221 ccaccgaaga aacagactaa cagtttttct tgtctttttc ttaaaaaaac tggactgtgt
     2281 cagcgaagaa gattcgttga tacagtccag tttgtctcta tttgcaccta tcgttattga
     2341 tgttccacat gtttgagcgt ctggttcaaa atatccagta cgtgagaatc atcaagacta
     2401 tacaaaatcg cttttccctc acgatgggac ttgacgacat ggttttctct taataaacgc
     2461 agttgatgcg atacagcaga ttgttccatt tctactgctt gggtgattgc tgtcacattt
     2521 tgttccccgt ctttcagata cagcagaatt ttgagacgag tggggtcact caacattttg
     2581 aatagttggc tgactttctc tacttttgtt ttttgcgtgt ctggcaatcc attcacctcc
     2641 aacttgaaaa aagactgact tctaaagttt accacttttt ccatcagtct caagtttttt
     2701 tattcttctg tcattttttt gataatcgct tcataagcgg ctactttttc agctgcgtct
     2761 tctgaagagt ttccttttac accgatataa aatttgattt tcggctcagt tcctgaagga
     2821 cggatagcga tccagccatt ttcttccagc gtatacttca atacgtcaga tggcggtgtc
     2881 gttagttttt ctacgtttcc ttcacggtct gttctcgtca actctttgaa gtcttctgtt
     2941 tggatgactt ttgctccggc aaattctgtc ggtgcttctt cacggaattt ttccatcaaa
     3001 gcagagattt ttgcagagcc ttcttgtccg ctcattgtca cagaaatcgt tttttctgcg
     3061 tagtagccat atgtttcaaa aatcgtttgc aagccgtcat acaatgtttt gccttgtttt
     3121 ttatagtaag ctgctacttc tgctaataag agcaatgctt gaatcgcgtc tttgtcgcga
     3181 acgaaaggtt taactaaata accgtaactt tcttcgaatc cgaacataaa agtatgggaa
     3241 tgatcttctt caaattgttg aattttttca gcaatgaatt tgaatcctgt caatacattg
     3301 aacatggtag ccccatagtg ttccgcaatt gctgtaggca attcgctaga aacgattgat
     3361 ttcaataccg ctgcattttc aggtaaagtg cctcgcgatt ggtgtgcttc caaaatatat
     3421 tgagcaaaca gggctccgat ttggtttcct gtcaatactt cataggagcc atttggcaaa
     3481 cgaacggctg cgcctaaacg gtctgcatca ggatccgttg caatcagtaa gtcagcgcct
     3541 tctttctcgc caagtcgcat cgcatactcg aaagcagagt gttcttctgg attcggtgat
     3601 ttgactgtag tgaaatccgg gtctgcgaca gcttgttctg gaactagtac gaattgttcg
     3661 aatccagctt gtttcaacgc acgttcgccc aacatttttc ctgttccgtg aagtggtgtg
     3721 tacacaagct tcaaatcttt acccattgtt tccaccaatt catgatcaat cgttactgct
     3781 ttgatttctt ctagataagc ggcatccact tcttctccga tgatcgtgat caatccgcta
     3841 tgttcagctt cgtcgtcaga taatacttca acttttaacg gattgctgac ttcacggaca
     3901 tatttcgtca atgcatctgc gtctgcagga ggcatttgtc ccccatcttc tccgtagact
     3961 ttatatccgt tgtaagctgc tggattgtgg gatgcagtta ccatgattcc cgcaaacgtc
     4021 ttcaaatgac ggactgcaaa agaaagctct ggtgttggac gtaaactttc gaatacataa
     4081 gaagggatat tgtgatgtgc taatgttttg gctgcttcca tggcaaattc tggtgattga
     4141 tggcgtgagt cataagcgat agcgacacct cggcgttttg tttcttcacc ttgtgtatcc
     4201 atgaaacgtg caagtccttc tgttgcttgt cttaccgtat agatgttcat tcgattgatc
     4261 ccaggtccta agattccccg catgcctgca gtaccgaatt ccaacggtgc ataaaaagca
     4321 tcttcaagtt tttcaggatc ttcacccaga tcatttaatt gtttttttaa gttttcttct
     4381 aaattttctt catgtaccca ttgttcatac acttgttccc aagacatgaa aataacacct
     4441 ctttctgaaa tatggttctt aataagtata gcattcactt attagatatg aaagtatgag
     4501 gaccaaaaaa cgcttaaatc aagaaaagat tcttttatca agcacggtta caagtattcg
     4561 cataaatcgg attcagtaga tttcacgcaa aaaaaagagt aaaagaacaa ttgaaaccgt
     4621 tctttcactc tttttaaact tatgcagttg tttcttctaa agattccaag tatttataca
     4681 cttgttgtcc agcaatccca ccatcaccaa cagcagtagt gatttgtcgc aaatcttttt
     4741 cacgaacatc tccgatggca aaaacgcctg gtatattcgt cttcatttcg ttgtctgttg
     4801 gaatccagcc ggcttcgttc gtcaacccag cttctctaaa tggtgcagtt aatggatcca
     4861 atcctacata gatgaatact ccgtctgctg ccaaatcaaa ctcattgcca gttttgacat
     4921 tacgtgcttt cacacctgtc actaccattt catttccaac gatttcgtca acgactgtat
     4981 cccacataaa ggaaattttt tcattcgcaa atgcacggtc ttggatgatc ttttgtgcac
     5041 gtaattcatc tctacggtgg acgatgacta cttcagaagc aaattgtgtc aaatagatgg
     5101 cttcttctac tgctgagtct cctcctccga tcactactag acgtttgtta cggaaaaatg
     5161 caccatcaca aactgcacag taagaaacgc cacgtccagc aaattcttcc tcgccaggta
     5221 cgcctaattt acggtggata cagcctgttg cgataatgac tgtttttcct tcgtaagttt
     5281 tatcttcaca aataacttct ttgtggctgc cgtgatcctt gatatcttgc acgattccat
     5341 aagcattttc tgtcccaaat cttgaaacac cttcatacat cttataagct agatcaggac
     5401 ccatgattga atcgaaacca ggatagtttt cgatttctgc cgtattgttc atttggccgc
     5461 caggagcgcc ttgttcgatc atcaatacgg ataagtttga acgtgaagca tataaagcag
     5521 cagtcatccc agcaggccct gcgccaataa cgattacatc atacatatgt agattctcct
     5581 ttacttaatc aagtaagcat actttacagc caatgcgaaa aaaagtctat tgttctgctt
     5641 gcgtttctcg aatagcttta ttatactcga aaaatccgct gaaaaacaat agacttttat
     5701 aaatcattga tcaatcgttc gaaagatttt ctccatttga atcaattact tttttatacc
     5761 agttgaatga tttcttcttc gaacgggcta atgtgccatt tccttcgttg tcacgatcga
     5821 cataaatgaa tccgtacctt ttcttcatct cacctgtacc agctgatact aaatcaatac
     5881 atccccaagt cgtatagcct aatagatcga ctccatctaa ttctactgca tctttcattg
     5941 cctggatatg tgctgccaga tagtcgattc gataatcatc ttccacataa ccattctcat
     6001 cgggcgtatc gactgcacct aagccgtttt ctacaataaa tagtggtttt tgataacgat
     6061 cataaatatc attcatcgtt attctcaagc ctaatggatc aatttgccaa ccccattcac
     6121 ttgcttccag atatggattt ttgacagaag caaagatatt tcctgcagtc tgttccaaga
     6181 gttcgttgtc agtagtcgaa actcgagaag aataataaga aaaggagata aaatcaacag
     6241 tatgttcttt cagcaatgcg gcatccccag gttccatttt gatctgaatg ccatttcgtt
     6301 ccatttcttt caatgcataa gcgggatatt ctcctcgtga ctggacatca atgaagaaat
     6361 aattttctcg atctgacttt ctagaagccc aaacatcttc aggattgcaa gtgtatggat
     6421 aatatgctcc tgctgccagc atacagccta ctttgttctc cggatctact tcatgagcga
     6481 tcttcgttgc gatggcactt gcaaccaact catgatgtgc cgcttgatat ttgatttgtt
     6541 cttcattttc tccttcttca aaatacagcc ctgcgcccat gaatggtgca tgaaggatca
     6601 tattgatctc attgaatgtc aaccaatatt tgacaagtcc tttatagcga ttgaagatga
     6661 ctcggcacag attttcataa aaaccaacga gttctctgga acgccacgca ccatattctt
     6721 tgatcaaatg catcggacaa tcaaaatgtg taatcgtcac aagcggctcg atcccgtact
     6781 tttgacattc tttaaagaca tcctcataaa atttcaaccc agattcattt ggctcttttt
     6841 catcgcccat tggaaaaata cgactccaag caatggacat ccgataagtc ttgaacccca
     6901 tttctgcaaa taaagcaata tcttccttat aatgatgata catatcgatc gcaatttttg
     6961 ctggataaaa atgattttca tcaaattcaa acattttctt ttttccagta atgacaggaa
     7021 aacggtcttc tccgattggc acaacatcta cgttcgctaa cccacgtccg ccttcatcat
     7081 aaccgccctc gcattgattg gctgctgtag ctccgcccca taaaaaatct tttctgaatc
     7141 ccatcatact tcctccatta catgtgtatt gcttgataat tctcttgcca gttcatcttg
     7201 gacttcgatt ccttctgctt ttaaccggct gattgtttcc ttgaattgcg gtaaatactg
     7261 ctgatgtgca ataaacaact catccatgat ccgttttgct gtctttcctg atcgtataag
     7321 cggattgatc gtaaacgctt gcaaagctgt tccataatca ccagtcacgg ctgcttcgat
     7381 cgtcagcaat tccatattct tcattacttg caaccagcct ctttcagcag caggaaggga
     7441 accaaacgct acatttcgtg cgccttgtgc gccaacatag gcagtgactt ctactacaca
     7501 atctgccggc agatcaggaa cagctccatc attctttgta gataccacga tctgtgtttc
     7561 tttatttcca tagatcgaag caatcgtttc acaggcagca tctgaataat aagcaccacc
     7621 tcgtttttgc aattgttcag gtttatgatc caattctgga tctttgtata attcaaacag
     7681 ctctgcttct gtttctttca cttgctgcgc tcttgtacca atcgtttgat attcttctaa
     7741 agcatgttcc agcatttctt cttcacgata gtaatagcgg tggtagccac aagggatcat
     7801 gttcatttgt tttaattgtt ctttgaaaaa cgggatgtca aagatatttt tcggcaatcc
     7861 attgtcttca tcgtataaac gatcaatcaa ttctgccgtg cggtcatttc ctttattatc
     7921 tgctactttg tgccaatgga agtgatttag accggcaaac ttgtagatca gctcttcttt
     7981 tttcttgcca agcattgttg gttctgtcat catcgccatt actggcacat tgcataatcc
     8041 gatcacttta tcccatttgc catatctgat aactgcttct gtcaccatcc cgcttgggtt
     8101 cgcgaaatta atcagccaag catttggaca taaacgcttc atgtcttcaa cgattgccaa
     8161 aaccactgga acggtacgaa aagctttgaa tatcccgcca gcaccatttg tttcttgccc
     8221 aatcatccca taataactag ggatccgctc gtctttgatt cgtgcatcta attgaccgac
     8281 acgaaactga gtcgtaacaa aatccgcatc tttcaatgct tcttcccgat ccagagttgc
     8341 atagattttg acttcatacg gagaagcatc ccacatacgc tgagacatct gactgacgat
     8401 ctcaagcttt tcttttcctt cttctacatc tacgagccag atttctcgta tcggtaattc
     8461 ctcatatctt ttgataaatc cttccatcaa ctcgggggtg taactgctgc ctccaccaat
     8521 cgtagcgatt ttgattcctt ttgccatgtt ctcactcctt tactttttac acttttatta
     8581 tcttttttgt aaacaaaaca aacaatagaa attcgtttct ttccagtaac atttacaaaa
     8641 gttagtgttt tcttctggat ttttgtaaat atcttgtcaa aaaatgtctt tattaagcta
     8701 tataacataa atccgtttac aaatcatttt caaaaaagtt atgatttacg taaaaaaaca
     8761 agaagaaggt atctacatgt tattacgtga aaaaatcaaa gaaatctcct tttctccttc
     8821 agaaaatgaa gtgattcagt ttctcttaag cgattctcca gcacttggcg aaatgaccat
     8881 ccaagagatt gctgagcgat gttatgtcca cccgtccaca ctcatccgtg tagcaaaaaa
     8941 aatgggtttt gatggctggg tcagttttcg taatgctttt ttagctgaaa cagcctatct
     9001 tgaagggaat tttcaagacg tagatgcgaa tcttcctttt tccaccacag acggtattat
     9061 gacgatcgca aataaaatgg cttctttaga acagatgacg attcaagata cactttctct
     9121 cattcatcat gaccaactgc aaaaagccaa acaactaatg cttcaagcaa aaaaaatcgt
     9181 gattttcgcc agtaactcga atactttgat ttctcaagat ttcatgctga aaatgaagcg
     9241 catcaaaatg gatgtcacga ttgtcaccac ttttggtgaa tcgttctatg aagcgtacaa
     9301 ttgcacaagt gatatgtgtg ggattctgat ttcttatacg ggtgaaaaca atgtgatcct
     9361 tcgtaccgca gaaatattga aacaaaatca tgtgccaatt ttagctttga ccagtatcgg
     9421 tgattcaacg ctatcttcaa tgagtacagc cgctttgcga cttacgacac gcgaacgact
     9481 gtattcgaaa attggtaatt ttacgatcaa tacatcgatt tgttatcttc tagacgtgtt
     9541 atatagttgt gtttttgcag aaaattatca acagaattta gtccatctaa tttctgtagg
     9601 acaaaaattt gataaacgcc ctatcagctc agccatcatg aaagagcctt tcaattttga
     9661 aaataaagaa gtggactaaa aagaatagtt aagcctagtc cattctttca cctaaccgtt
     9721 caggtcagtg cggtgataag tgcatcgtct ggcccgaccg tttccccttg attttccaaa
     9781 atatccaaat aatccatgta attgatgaca atcacaggtg tttctaaact gtatccttcc
     9841 tcttcgattt tctttttgtc gaagcgaagc aacagatcgc cttgtttcac tctttctcct
     9901 tgctgcacca agctttcaaa atatttccct tccaactgaa cagtatccaa tccgacatga
     9961 atcagcaatt ccagacctgc ttcagaaacc atcccgattg catgtttcgt cggaaacagg
    10021 gacatgactg ttccgtcaaa aggggcacgt acttctcctt tttcagggta gatcaagatt
    10081 cctcgaccta gtgttccttg tgcaaaagct gcatcctttg ctgcattcag tggagcgatt
    10141 tgtccttgta tcggtgtttt tacaatttct ttacctgatc ttttctgtct atcatctgtt
    10201 tcatctgctt cctccttcca aaagaaaaaa gtcagcgcaa aggataccac catcgcaatg
    10261 gcgatcccaa tcaatacatt aatcacatct gttaaatcac catctggtct aatatagttg
    10321 ggaattgtaa agatgcctag tccaccttga ttccaagaag ttaaattcat taacccacta
    10381 tacgcgcccc ctaacgcacc accgacacag gtaaaaataa atattttttt acgtggcaat
    10441 gtaaacccat aaatagctgg ctctgtaatc cccacaatac cagaaacaat ggctggtatt
    10501 gcaattgctt tgtcttttgg atttctaagc ttaaagtaga gtgcggtaag tgctgcagtt
    10561 tgtccaaaag acactactgc aacaggtgtc ataatatttt gccaaccatt cacagaaaat
    10621 tgcatcaatc ccattggtac gattgcccaa tggagaccaa acatcaccag aatttgccag
    10681 aagaagccta cgattgctcc ataaataact ggcgaaagtg ctaaaagtgt ttcaaatcca
    10741 gccattaaaa tatcagttgc gaatgtgaat actggtccaa cgagtaaaaa acctaaaggc
    10801 attgtaataa gaacggtgaa gaatggaacg aagaaattcg cgatcatctc aggtactaac
    10861 tttttaattg gtttctgaat ttggctagct aacaacacaa taaagataat gggaatgaca
    10921 gaagaagcgt agttacgcgc aatccaggga atgccgaata ctgtttgata aataggtgct
    10981 tcaaaaatag tgccagaaaa tatggtcgtt agtggtgctt ctgctgcacc actcaaagct
    11041 tccagcgaca agctaggatt cattaatgct gcgccaagca acatccctac gaattcattt
    11101 actttaaatt tacgggcagc tgttaaaccg ataataacag gcataaattg gaatacagaa
    11161 tcaccgatat tatcaaacat aacaaatgtg ccagaaccgc ggtcaaacgc gcctaagaag
    11221 acaaggaatg ctgtcagtcc acgaatcatc ccgtcagcag ctaggatagc aaggaatggt
    11281 tggaaacagc ccgatattcc atcaataagc atatcaaacg gtctcatttt ttctgttgaa
    11341 acagcctcat caaaagtaag gtttcctgct tttataacct cttcaaacac gactggcaca
    11401 tgattcccga tgacaacttg atactgccct cccgctcgca ttaccgttac aataccgtcc
    11461 atatttttca agatctcatc atttgctttt tcttcgtttt ttaatttgaa acgaagcctt
    11521 gtgatacaat gtgtcaaacc attgatattt tccttcccgc cgacattttc tacgattttt
    11581 ctcgctaatt cttggtattt tcccatttct attccctcca atttattagt ggaaggttta
    11641 gccaactgaa tgtcactacc cttatttcat acgttcttgt taatttatcc tccttttttt
    11701 atttatttac aacgattctc atgcactaaa cgcgcagtat ggatcgttaa atacattttt
    11761 tcgtccgtgg a
//
LOCUS       DANKBF010000084        11731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000084 DANKBF010000000
VERSION     DANKBF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(364..1482)
                     /locus_tag="PWY89_002505"
     CDS             complement(364..1482)
                     /locus_tag="PWY89_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067503.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1953..3260
                     /locus_tag="PWY89_002506"
     CDS             1953..3260
                     /locus_tag="PWY89_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2067504.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="PWY89_002507"
     CDS             3379..4578
                     /locus_tag="PWY89_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2067505.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="PWY89_002508"
     CDS             complement(4605..5873)
                     /locus_tag="PWY89_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2067506.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6036..6682)
                     /locus_tag="PWY89_002509"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="PWY89_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="PWY89_002510"
     CDS             complement(7090..7593)
                     /locus_tag="PWY89_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2067507.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="PWY89_002511"
     CDS             complement(7716..8480)
                     /locus_tag="PWY89_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2067508.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="PWY89_002512"
     CDS             8588..8863
                     /locus_tag="PWY89_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2067509.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="PWY89_002513"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="PWY89_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2067510.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10630)
                     /locus_tag="PWY89_002514"
     CDS             complement(10430..10630)
                     /locus_tag="PWY89_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067511.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            11435..>11731
                     /locus_tag="PWY89_002515"
     CDS             11435..>11731
                     /locus_tag="PWY89_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2067512.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3560 a         2449 c         2076 g         3646 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaataacg
    11341 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    11401 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    11461 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    11521 ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca agcaaggtaa
    11581 gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt
    11641 cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac
    11701 gcctgacgtc attatcgggc gaaaagaaag g
//
LOCUS       DANKBF010000085        11436 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000085 DANKBF010000000
VERSION     DANKBF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            178..1134
                     /locus_tag="PWY89_002516"
     CDS             178..1134
                     /locus_tag="PWY89_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL2067513.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1224..2327
                     /locus_tag="PWY89_002517"
     CDS             1224..2327
                     /locus_tag="PWY89_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2067514.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2340..3293
                     /gene="menA"
                     /locus_tag="PWY89_002518"
     CDS             2340..3293
                     /gene="menA"
                     /locus_tag="PWY89_002518"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2067515.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4228..5574)
                     /gene="gor"
                     /locus_tag="PWY89_002519"
     CDS             complement(4228..5574)
                     /gene="gor"
                     /locus_tag="PWY89_002519"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2067516.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5708..6961)
                     /locus_tag="PWY89_002520"
     CDS             complement(5708..6961)
                     /locus_tag="PWY89_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2067517.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(6967..7890)
                     /locus_tag="PWY89_002521"
     CDS             complement(6967..7890)
                     /locus_tag="PWY89_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2067518.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(8111..10603)
                     /locus_tag="PWY89_002522"
     CDS             complement(8111..10603)
                     /locus_tag="PWY89_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2067519.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10610..11077)
                     /locus_tag="PWY89_002523"
     CDS             complement(10610..11077)
                     /locus_tag="PWY89_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL2067520.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(11213..11286)
                     /locus_tag="PWY89_002524"
     tRNA            complement(11213..11286)
                     /locus_tag="PWY89_002524"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11250..11252),aa:Arg,seq:acg)
     gene            complement(11344..11417)
                     /locus_tag="PWY89_002525"
     tRNA            complement(11344..11417)
                     /locus_tag="PWY89_002525"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11381..11383),aa:Pro,seq:tgg)
BASE COUNT         3390 a         2335 c         2005 g         3706 t
ORIGIN      
        1 cttttgtcta actccttttg gatttttttc aaattttaaa ataaagcgtt ttttcacgaa
       61 atttgctgaa agaaaacgtt tgaaatatta catgggttat ttatcaaaaa tagttgactt
      121 ataggctaaa aagattagaa tgtataatga atcacaaaga aaaattgtgg actagaaatg
      181 gaatggtggg gtattaaaat gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca
      241 ttttcagctc tagtgttagg cgcttgcggc gcagatgaca acggaagctc aaactcttct
      301 tctgaatcat ctacagcaca atcttcgact gcaaaaagca caacagaatc atctgcaaaa
      361 gttgtagctg gcggagattt gcaagacggc acatataaat tagaagaaaa aaattattca
      421 aatggttacc gtgcagtctt tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa
      481 tatgataacg tcaatgaaaa cggcgaatca aaaacaaaag atgcagaata caacaaaaac
      541 atggaagcaa aatctggcac aaacccagaa aaatacattc ctgagttgaa tgaccaattc
      601 ttaaaagcac aaagcgcaag cggtgtggaa gtcgtaactg gagctactca ctcatctgaa
      661 agcttccaaa actacgcaca acaattgatc caagctgctc aagctggtaa cacagataca
      721 atcgaaatcg acaatggtgc tgatttgaaa gacggcactt acaagcttga agaaaagaac
      781 tactctaacg gctaccgtgt gcaattcgaa atgacagttg ctggcggcaa agtaactgaa
      841 tcaaactttg attatatcga caaagacggt aaatcaaaac aagatgatac agaatataat
      901 gaaaatatga aagcaaaaag cggtacagaa ccaaaaactt acatccctac tctgaacgac
      961 gaattcgtca aagcaatggg cgaagaagat ggctcacctg ctaatgtaga agttgttaca
     1021 ggtgctacac acagctcaca ttcattcatc atgtacgcac aacaattagt gaatgcagct
     1081 gaaaaaggcg acactcaaac aatcgaagta gataatatcg taacagaaaa ataatcacat
     1141 aatcatacta ataaaagttg gagggtggga cgatagccag attaggcagt cgtctcacct
     1201 ttcaaaatga agagaggaaa aagatgcgga aaaaactact attttcctta atcacactgg
     1261 gcatctcttt tatggtgcta actggctgca gcaactcagc aaataaaaca accactgaaa
     1321 gttcagcagc taccaagata ttgaaagatc catattctga tgaacagttc ttgttaggga
     1381 cttacgttcg tatccgtgtg tatgatgaag gcaaagaatc agcattgaaa cctgccttcg
     1441 atcgagtaaa agaacttggt gataaaatca cgatcaatca aaaaggttct gagatcgatg
     1501 aagtgaatga gcaagctggg atcaaaccag taaaagtttc tgatgacgtc tacacgttag
     1561 tcaagcgggc ctacgaatat agccaagatt ctcaaggcgg attcgatatg gcgattggtg
     1621 caatcacaca attatggcga atcggttttg atgacgccag aaaaccttcc caagaagaaa
     1681 tcgatcaggc attgaaatta gtggattatc ataagatcga actaaatgac aaagaaaaaa
     1741 ctgtttatct aaaagaaaaa ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca
     1801 ttacggacga agtagtgaaa gtcttgaaaa aacaaggagt gaccactgcc atcgttgact
     1861 tgggcggtaa tgtctatgtc ttaggccaca gcccaagagg cgaaaaccaa gactggacag
     1921 tcggcatcca agatccgaac atggctcgcg gatctgtttt aggttctatt aaagaacgga
     1981 acaaaacatt agttacctct ggaatttacg agcgttattt agaagtagat ggcaaaaaat
     2041 accatcattt gtttgatcca aaaacgggtt atccgttcga caatgatatc gcaagtgtca
     2101 cgatcataac agataaatca attgatggtg acgggttgtc tacagcagtc ttttcaatgg
     2161 gtgtgaaaaa aggactggaa tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct
     2221 tcgtgacaaa agacgataaa gtatatgtga ccgatccaat caaagacacc ttcaaattat
     2281 cggaggactc ccattacacg atgggtgacc gttctgaact aaaataagga gtacgaacca
     2341 tgacactaaa aatcttttta gaagtagttg aaatacgaac aaaggtggct agtgtatttc
     2401 cttttgcggt aggggtatta ttctctatcg ctttttttca tgagatacat tggctaaaca
     2461 cttttttatt ttttattgga atgctagttt ttgatttagc gactaccgct atcaataatt
     2521 acatggatta tgaaaaggct cattctgaag tatataagta tgaagaaaat gtcattggcc
     2581 gcagaaatct atctcctgta ttgattcgaa acatgatttt agctatgatt gcatttgttt
     2641 ttgttatcgg cgttattcta acgatcaata cgggttggct tttacttttg atgggattta
     2701 tctgttgttt tattggagta ttctatacat tcggaccgat tcctttatct cgaatgcctt
     2761 taggggaaat tttcagtggg gtgactatgg gccttggaat ttttgccatg gtcgtttatg
     2821 tcaatacatt cggttcagat gtctatgatc tgacagtcaa cttttcatct ggacaatttg
     2881 ctttggtagg aaatctttgg gctatagcag caatcgtatt agcttcctta cccttggtgt
     2941 ttacgattgc caatatcatg ttggctaata atttgcggga tcttgaccgt gatattgaaa
     3001 atcatcgcta tacactagtt ttttatatcg gacgtcctgc gggaacagtc ttattccagc
     3061 tattgatgta tgcttgctat ttcgtgattt taatggggtt gttgagcggt atctatgaat
     3121 ggccgatctt ggtcaccttc ttgaccttgc caattgtgaa gaagcatttg gatcaattca
     3181 agcaaacgtt accccagcct atcagtttca gctattccat caaaaatatg gtcttattca
     3241 atgccagtta tgtattgggg ctattactaa caatcattat gcaagccatt taacagcatg
     3301 aagaggttgt gaaaaaactg ccctgcatca agtaataaga acagccaaat aaaaatagct
     3361 ccttatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     3421 atcaggaggt tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg
     3481 gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt
     3541 ttatcagagg ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
     3601 ggctttccac atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg
     3661 tttatcagag gttgtaaaat cagtgttttg tcctgagtat tagagaaaca aatggaaaaa
     3721 tagttcctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacta
     3781 tttgttcgga aaaagtgact aactatgaca caattttttt cacagtccct tttttcactt
     3841 gatttgattc gtaccagcga acttgacgag aactttcttg gtttggtaac tatatggtat
     3901 tcttttaaca tcctcgtttg cttcttctaa tttatttata caaagtcaca cttttttaaa
     3961 catctgttct tcgatacatt tctatttctt ttaaacatcg ataccaatat tttgaactta
     4021 agagtgcata ccaaatggat tcatttactc ttcgtcagtt acgaagataa tcaaaatctc
     4081 caacttccct acttatttcg ttttgagaat tatcgaagca atcttacata aataactaaa
     4141 aaacaaaaaa ccagaaaatc agtatactga ttttctggtt ttacatggtt tgttcaataa
     4201 aaagctgttg gaactgttaa tattaaatta tcgcatcgtt acgaattctt ccgctcctgt
     4261 tggatggatg gctactgtat tgtcaaagtc ttctttagtt gcacccatct tgatagccac
     4321 ggcaaaacct tgcagcatct catcgacacc aacaccaata gcatgtaagc cgacgattct
     4381 ttcttctttt ccgacacaga tcaatttcat ttcgcatttt tggcgataat catttaatgc
     4441 aaagtacatt ggggtaaagc gtgaacgata aactttcaat tcattttcgc catatttttc
     4501 caatgcttct tcttctgtca aaccaattgt tgcaattggt ggatgcgtaa agacgactgt
     4561 cggaacaagt ttatagtcaa gataagaatc tttctttcca ttaaataaac gttctgataa
     4621 acgtctacca gctgcgattg caacaggagt cagatctaat ttaccgatca catcaccaac
     4681 agcaaaaata tgttcatccg ttgtattttg gtatttatct actttgataa accctttttt
     4741 gtccaattcc acatttgtat tttccagacc taatttttcg gtattcggta cacgtccacc
     4801 ggcaaaaagt acacaatcac cagttaaggt ttcaccattt tcaaatagca ccgtatattc
     4861 gtcattgttt ttagtaattt ctttagcaat atgatgtgcg taagttttta ttccagcttc
     4921 ttggtacatt tggatcaaat tgtccgaaag cattttatca aacgttctaa gtggtcgttc
     4981 ttttcgatat gcccacatga cttcactacc taaaccacga aatacacctg aaagttcagc
     5041 cgcaatgtat ccagcaccta aaacgattgc tctcttaggt aactctgtta attcaaaaaa
     5101 tccatttgaa tcaattgcgt attctccacc aggaatggtc attttacttg cacggcctcc
     5161 agtagcgatc aagattttag gagcacgata ggtttcaccg tttacttcga tcgtttgact
     5221 atcgataaaa gtagcgtaac cttttatagc ctcgacatga ttgctatcta atcctctttg
     5281 ataagcaccg tgtagaaaat caatatattt ttctcgtctt tctactagac cggcgaaatc
     5341 aacatttttt aaatctgctt ggataccgta acttgaggca tctcgttcaa ttgtttccaa
     5401 aatcgtactt gcttgccaca taactttttt aggtacacaa ccgacgttga cacatgttcc
     5461 gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca tgcatccctg ctctgtttgc
     5521 tgaagcaata ccgccagagc cgccaccaat tacgatgtaa tcatatattt gcattttgtt
     5581 cctccttatt gaagagatca atcaaaacct ttatttaacg atcctttttc tacagaaaac
     5641 aagtgtaaca cttttcttga attttgtatg cgaagatgct taaaacaggg atatatcagc
     5701 tttttcctta tttacgtttt cggatcatat ctccaggttt caccggctgt gcgactggca
     5761 tagttaaaat catcattgga tttggtgctc ggtcaatgga ttcgccttct tcattatgca
     5821 taacttcaac ggtttgactg aaatgactga atcctggacc ataaaattcg atttcatctc
     5881 ctacagaaaa caagtttctc tgacgtatcg tagcaatctg tgttgcctca tcataagata
     5941 atacttcacc gacaaattta tatactggga ttttacgacg ttcaccgaat aattgttcgt
     6001 tttcagttgg tgtattgtag taaaaacctg tagctaattc tcgttgtgct actttccata
     6061 actcgtcgat ccattcttgc ttgcagacat agttttcagg atcttccata taactgtcta
     6121 cagccgcttt atagacattt gacaccgtag atacataatg gatcgatttc attcttcctt
     6181 cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg aatatgttcg atcattgaca
     6241 tatccactgc actcatcgag aattcttctt ccacttcgcc tgtatcagtt aaactgtttc
     6301 gttcttgacc aaaaggcata tcgaacaatc catacttcca gcgacaagat tgagaacatc
     6361 cgccacggtt tgcatcccgc atcgacatat ggttggacaa tgtgcatcga ccagaataag
     6421 agatacacat ggcaccatgg ataaatgctt cgatttcggt atccgtattt ttacgaatct
     6481 ctgccacttc ctccattgaa acttcacgag ctaaaacgac acgttccaac ccttcttctt
     6541 tccagaattc caatgtctca aaattagttg ctgaagcttg agtggataaa tggatcggca
     6601 gacctggggc ttctgtcgca caaatttcga tcaaagctgg atcagaaaca atcactgcag
     6661 aaataccgac atcacgcaaa ctgcggaaaa attcccctgc tccatcttca tttccttcat
     6721 gggtgaccat attagcagca acgtatacct tcgcatcatg ttcttttgca aaagcaactc
     6781 cttcagccat ttcttcataa gtaaaattac ctgcacggct tcttaaacca tatgcattcc
     6841 cgccaatata tactgcatct gctccataat ggatcgcggt ttttaacttt tccagtgttc
     6901 ctgctggcgc taggatttct ggtcgtttca attttctttt tactgtcatt tctttcatca
     6961 tactccttat ttgatagcag aaggatcaat ataaaagaat cctgtatcta attcacggtt
     7021 ttcaggatgc aatttactga tttcgctagt cattttttcc gctttttctt gatcccagct
     7081 accttctaaa atttgttttt ttgcttcggc gaatagtcga gtgatgttta cgaatttttc
     7141 gcctggagta aataatccgt ccaatttcca tgtacagtaa tgatgctgat aaagttcatt
     7201 caattcattc gagagattca gatcattgct ggcaaaaata tgtgtgccat gactatcttc
     7261 ataaatagaa taatgtgtct cttctttttt aggttccgag ataaaaagac ctctttcacg
     7321 atctttttgt tcgtcttgtt tcgtatagtt atagtaattc tgtagcagtg gacgtttgga
     7381 ttgatggatg catgtagcac catacacaag ggtttctaca ggaatatcga gtttttcttc
     7441 catcgccttc atctcttcaa aaggaacttc acgagcaagt actgctccaa ctgcgccttt
     7501 tttcgcccag aaattgattt gacgtgcgct cgttactaat gtttcagcat cataaatata
     7561 cggtatacgc aattcaggat tttttttcat cgtataaata atacctgtat caccaactgt
     7621 gatcacatct acgtttattt cttttaaaaa agccaaatat tcaggtacat ttttcatttt
     7681 ttcaggatga atgatcccat ttacagcaat attcgctttt ttaccatact tgtgtgctaa
     7741 ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca ggcagacgca atccaaattg
     7801 ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc aactgttctg cttgttcaat
     7861 agtttctact gtggttatga gttcgatcat aattttctcc tcctacaact tcataatagt
     7921 agcatagctc taaaaacttt ccaatacttc ttctatttaa agtcggattt ttgaaaatta
     7981 tttcacaaaa aatcaaaata aagttcgata tattctgtcc aaatataaaa agagattgtg
     8041 acaaaaatct tgtcacaacc tcttaatcaa gtcttactat tataaaaatt cagctacaca
     8101 ggccaagtaa ctatagggaa cctactgttt cctttttagg tccgcgaaca ttcaatgtga
     8161 tcttaccttt tgaagcacct actgttacgt ggtcacctaa acgaatttct ccgccaagca
     8221 aagcctcgct caatcgatct tcaatttctt tttgaagtgc gcgacggatt ggacgtgcac
     8281 catattcagg atcaaatcca gctttcccga tcacatcgat tgcagatggt gtgattttca
     8341 cttgaatatc ttgttctttc aatcgtttga taatcgcttt gctcatgatt tggacgattg
     8401 tatggatttc atctttactc aatgaatgga agacgacagt ctcatcgatt cgattcaaga
     8461 attcaggtcg gaaagctttc tttaactctt ctaagatacg tttttgcatg gctttgtgat
     8521 ctttagtaac atcttggaca ttgaatccga catttttttc ttctcgaatt tgtgtcgctc
     8581 cgatatttga agtcataatc atgattgtat ttctaaagtc cacttttcta cctttagaat
     8641 ctgttaaatg accatcatcc aatacttgca gaagtaaatt gaaaacgtct ggatgtgctt
     8701 tctccacttc atctaagaga atcactgaat atggtttttg acgaattttt tctgtcaact
     8761 gtccaccttc atcataccct acgtatcctg gaggagaccc gattaatcgg cttgtactgt
     8821 atttttccat aaattctgac atatcaacac ggatcagggc atcttcactt ccaaacatga
     8881 cttctgaaag tgctttggcc aattctgttt ttccgacacc agttggtcca aggaacatga
     8941 atgaaccaat tggacgatca ggatctttca atccgcttcg tgcacggcgg atcgcacgtg
     9001 aaactgcttt gacagcctca tcttgaccaa cgacacgttc atgcagaagt ccttccagtt
     9061 ctaacaagcg ttcgctttct tttttctcca actgctgtag agggacacca gtccactccg
     9121 atacaactgt agcaacatct tcttctgtta cacgatcaga ataaccttgc tcctgttttg
     9181 cttcaattgc agaaactttt tgcagctttt tacgtagtgc tttttcttgt ctgcgtaatt
     9241 gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc cttttcttgt actagttcat
     9301 cgatttctag tttgatcaca gttgattttg ttaagtgatc ggtttgatcc aaacggactt
     9361 tcgctgctga ttcatcgatc aaatcaattg ctttatcggg tagttgacga gaagtgatat
     9421 aacgaacaga aagattgact gctgctcgta cagcttcatc tgtgatctcc actccatggt
     9481 gctcttcata tcttgtacgt aatccttgaa gaatcacttc tgcttcttca ggagttggct
     9541 catctacttg aatgcgagca aaacgtcgct ctaatgcaga atctttttcg atatattttt
     9601 gatattcatc tagggtcgtt gctccgatcg tttgtaattc tcctcgagct aacgccggtt
     9661 tcaagatatt cgaagcatca attgctcctt ctgctccacc tgcaccaatt aaggtatgca
     9721 attcatcaat gaataagatt acttgaccat cattatagat ttcgtcgatg acctttttca
     9781 ttcgatcttc aaattcaccg cgatatttcg taccagcaac caaagatccc atatcaagca
     9841 tcatcaggcg cttttcttgc atgtcttctg gcacttcacc taaaatgatt ttttgagcta
     9901 acccttcagc aatggctgtt ttgcctacac caggttcacc caccagtaca gggttattct
     9961 ttgtccgtcg gctaagaatc tggataagcc gtttcacttc tctcgaacga ccaacaactg
    10021 ggtccagctt gttttctttt gctaatttcg tcaaatcacg tgctaatgaa tctagcgtag
    10081 gtgttccttg cggtgtttgc cgtttatctt gtacatttac atttctacga cgattcattc
    10141 cttttccagc agaatcacca gctagcccca ttttttgttt cagaacttga cgggcttttg
    10201 aaaggctgat acctaagttg atcatgattc gagtagccat gatactttca tcttttaata
    10261 agctcatcaa aatatgttct gttccaactt tttgagaacc agttttcttc acttcgctgc
    10321 tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg aagaaagact cctttagggt
    10381 aagaacggat accaccataa ccaatcagat gttcaatttc ttcataaatt tccgtttcat
    10441 tcaaatccat ttcacgtaat acttttccag caattccatt ttgctcgacc actaaggcta
    10501 gtaaaacatg ttctgaacta actgattgat gtttcaaccg ttttgcttct tcttgtgcaa
    10561 tttctaatac tgcttgggct ctttcagtaa aaagttcttc catctaatct cacttcccat
    10621 cttcataact taaacgttcc agaaatgctc ggagaatccg agcacgtgta tgatcttcat
    10681 attcattgaa attcaatgtg ttttttgcaa ttgcactcaa catcaagttt ccttcttttt
    10741 ttgtaattat ttggtcttca taaagttttt gaataatagc aaaagcattt ttctcactga
    10801 ttgtatcatc aaataactga tttatctgct caagtaattg ttttttatct gaaatcctta
    10861 cttttgcaat tcgtatataa ccaccaccac cacgtttgct ttctactaga taaccacgtt
    10921 ggatggtaaa acgggtatta atcacatagt tgatttgtga aggaacacag ttaaagaggt
    10981 ctgccatttc aatccttcga atctcgatca tttcactatt ttccagaatt ttctttatat
    11041 atgattcaat taaatccgag gtattctgat gtgccatctt attcattccc tttgactaat
    11101 attgacctta attataacga atttgataat agaaagaaag aagaacgctt tttgttaaat
    11161 aaatccttta gaatcaacaa aaaaaaatac ggaatatact attccgtatc gacgcgccca
    11221 agaggagtcg aacccctaac cttttgatcc gtagtcaaac actctatcca gttgagctat
    11281 gggcgcatat tcaatttaaa aaaattataa atcatttgaa aacaaaaagc aagataaaaa
    11341 aaatcgggaa gacaggattc gaacctgcga ccccttggtc ccaaaccaag tgctctacca
    11401 agctgagcta cttcccgttt aaaaaataaa aaagac
//
LOCUS       DANKBF010000086        11197 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000086 DANKBF010000000
VERSION     DANKBF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(13..906)
                     /locus_tag="PWY89_002526"
     CDS             complement(13..906)
                     /locus_tag="PWY89_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2067521.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="PWY89_002527"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="PWY89_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2067522.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="PWY89_002528"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="PWY89_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2067523.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="PWY89_002529"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="PWY89_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2067524.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="PWY89_002530"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="PWY89_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2067525.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="PWY89_002531"
     CDS             complement(6343..7062)
                     /locus_tag="PWY89_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2067526.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="PWY89_002532"
     CDS             complement(7059..8372)
                     /locus_tag="PWY89_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2067527.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="PWY89_002533"
     CDS             complement(8453..8773)
                     /locus_tag="PWY89_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2067528.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="PWY89_002534"
     CDS             complement(8881..9168)
                     /locus_tag="PWY89_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067529.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="PWY89_002535"
     CDS             9295..9939
                     /locus_tag="PWY89_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2067530.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="PWY89_002536"
     CDS             complement(9998..10705)
                     /locus_tag="PWY89_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2067531.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="PWY89_002537"
     CDS             complement(10925..11095)
                     /locus_tag="PWY89_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067532.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DANKBF010000087        10629 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000087 DANKBF010000000
VERSION     DANKBF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..568
                     /locus_tag="PWY89_002538"
     CDS             <1..568
                     /locus_tag="PWY89_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067533.1"
                     /translation="YDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPL
                     LSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLEN
                     DFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCE
                     HYNRLSEEKILLPENWAELRKLVEYGII"
     gene            555..1976
                     /locus_tag="PWY89_002539"
     CDS             555..1976
                     /locus_tag="PWY89_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2067534.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1989..2996
                     /locus_tag="PWY89_002540"
     CDS             1989..2996
                     /locus_tag="PWY89_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2067535.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            3008..4012
                     /gene="neuB"
                     /locus_tag="PWY89_002541"
     CDS             3008..4012
                     /gene="neuB"
                     /locus_tag="PWY89_002541"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL2067536.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            4009..5157
                     /gene="neuC"
                     /locus_tag="PWY89_002542"
     CDS             4009..5157
                     /gene="neuC"
                     /locus_tag="PWY89_002542"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL2067537.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            5130..5807
                     /locus_tag="PWY89_002543"
     CDS             5130..5807
                     /locus_tag="PWY89_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2067538.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            5797..6939
                     /locus_tag="PWY89_002544"
     CDS             5797..6939
                     /locus_tag="PWY89_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2067539.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            6952..7929
                     /locus_tag="PWY89_002545"
     CDS             6952..7929
                     /locus_tag="PWY89_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2067540.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            7929..8837
                     /locus_tag="PWY89_002546"
     CDS             7929..8837
                     /locus_tag="PWY89_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HDL2067541.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            8838..9590
                     /locus_tag="PWY89_002547"
     CDS             8838..9590
                     /locus_tag="PWY89_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2067542.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            9595..10542
                     /locus_tag="PWY89_002548"
     CDS             9595..10542
                     /locus_tag="PWY89_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2067543.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         3848 a         1344 c         1962 g         3475 t
ORIGIN      
        1 ttatgatgta aaaaatataa aaaaaattcc caaaatagat gaaaacagta aattgatgaa
       61 aaacatacaa tcaatctttg ggatagaaga agtttttgtc ggagataaat gtatcttttt
      121 tgatcaacct ttattatctg atggttatgg catagatgaa acagatattt tcgctaaagt
      181 aaatagtata ttttctggaa aaaaagaaac tttaattaag cttcatccta gagcaaggac
      241 aaatcgttat ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact
      301 tgaaaatgac ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc
      361 atctatgatg tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga
      421 atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga
      481 acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag
      541 aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca
      601 gtttctggta tactataggg aattttttta ttaagggaat ttcattcata actattccta
      661 tttttgtaaa tattactact caagcagaat atggtttgat taataatttc acttcatttg
      721 tatcaatttt ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt
      781 ttgactataa agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt
      841 cgttaattgt gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg
      901 atatgaacca acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta
      961 gttatatcca gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt
     1021 ttatatctac ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg
     1081 atgataaata tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag
     1141 taatttattt aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat
     1201 acgctttaaa aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac
     1261 aattcgatag aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct
     1321 acattagtaa cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg
     1381 ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta
     1441 actattattt agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt
     1501 tagcaaaatt tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa
     1561 ccttaggcta tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga
     1621 aaaaaacatc gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa
     1681 acatgatttt catacctagg ttaggaataa ttgctgctgg atacacaacc gtatttacgt
     1741 attttttact ttttattttt cattataata tcgctaaaaa aattacgaat aaacaattat
     1801 ttgacacaaa aaaaatatgt atcataataa tttttgtatc aattttttct ttgatacttc
     1861 atattttaat caatcaaatg ttgattcgtt acgttttgac atcattattg ataattagtt
     1921 tagttatata ttttttaaag attaagaatc gaattataga tgactataat tcataggagg
     1981 agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt tccattggaa
     2041 gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt tatagtagag
     2101 gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat ttgagatttt
     2161 taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat gtggatatta
     2221 tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct atggaagctg
     2281 taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa aataatgtaa
     2341 aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc atgggtgcga
     2401 caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga aaaacagaaa
     2461 caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct gttattcctc
     2521 tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt gatatgacac
     2581 gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct atgaattcaa
     2641 aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact ttagctcaat
     2701 cattaataaa aatatataat gatacacatg atgaaaaata tagaggtgaa ataaaagttg
     2761 taggtatcag gccaggagaa aaattatatg aagagctaat gactacagac gaaattgaac
     2821 gctcatatga aaatgataaa atgtatatca ttccaccaca ctatcatgct actgacgaac
     2881 tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc ttcagatgca acagaattaa
     2941 tgagtggaca ggaatttatt agaatcgcaa aagagaataa tgtcgtgact tggtaaggag
     3001 ttttatgatg aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag
     3061 tctagattta gctttagaaa gtattgataa agctaaagaa tgtggagcta acgcggttaa
     3121 atttcaaaca tttaaaacga ataagctagt ttccaaaaag gctccaatgg caaaatatca
     3181 gaaggataat cttaaagagg acagttctca atttgaaatg ctaaaaaaat tagaattatc
     3241 tcaatctgat tttattagaa taaaagatta ttgtatacaa aataaaattg attttttgtc
     3301 tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa
     3361 aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt
     3421 gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg
     3481 tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga
     3541 agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta
     3601 ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt
     3661 aattgagaaa cattttacct tggataagga actacctggt ccagatcata aagcttcttt
     3721 agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg
     3781 tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag
     3841 cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat
     3901 aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt
     3961 taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata
     4021 cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta
     4081 ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat
     4141 ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt
     4201 ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt
     4261 ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg ggatagagga
     4321 gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac
     4381 ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca
     4441 tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat
     4501 tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta
     4561 cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt
     4621 ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca aattttagct
     4681 ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc taatagtgat
     4741 gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga tattatttat
     4801 attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa atttatgatc
     4861 ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt aataaattta
     4921 ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa tctaaatgaa
     4981 aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa aagcttgaaa
     5041 aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa aacattgaat
     5101 caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc ttattaaaat
     5161 atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc attattgaaa
     5221 ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa aataaaatgc
     5281 aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat agcaaaaaaa
     5341 aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag attataaaaa
     5401 aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct atagtggcaa
     5461 aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt aacccagatg
     5521 ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat gattgtagaa
     5581 ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta catgtaggtg
     5641 taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt ggatcttggt
     5701 gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc ttagcttatg
     5761 gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat tttattatca
     5821 agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa agataattgg
     5881 atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa atatactggt
     5941 tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct tgccttaaga
     6001 ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt tatagctaca
     6061 gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga acctaataca
     6121 tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag aaacaaccaa
     6181 ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta tgaacgaatt
     6241 ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga atcgttaggt
     6301 tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta ttcatttaac
     6361 ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga taaaaaatat
     6421 gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta ttatcaacat
     6481 agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg acgtggacaa
     6541 atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg atataaggaa
     6601 gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat ggtgccaaat
     6661 cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt agttaacgta
     6721 ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc actacatttg
     6781 caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag tgtgtcagaa
     6841 aagttattta atagcggctt atgcttacct tctggaagta atatgacaga tagtgaacaa
     6901 gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt tatgaacata
     6961 ggaattattg gtttaggttc catggggaaa agaagactta gactcattca agaacacttt
     7021 aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga agttgaaaag
     7081 gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt tgaaataaat
     7141 tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga ggctttaaat
     7201 ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga tgaaataatc
     7261 gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt gaagagaaaa
     7321 gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta tcaataccat
     7381 gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt ttttgtttca
     7441 aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg gattatagat
     7501 acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa tcttgatatt
     7561 gattattcgg acagtttcac tcttattatt gagcatgaaa ctggtatagt tggaacttta
     7621 agtgtagatg ttgtttcaag agtagctaaa agaaatttaa ctataattgg tgaaaagatt
     7681 ttaatagaat gggaaggtaa cccggattct ctattttcat ggaatgaaga aacttctagt
     7741 atgacagata ttcaattgta cgatgaggtt atgcatgata ataattattc acatactatt
     7801 attgaagatg catatttaga ggagattaaa gaatttttca cgcttttgga aaagaaaatg
     7861 gaaccccaat atacttttga aaaagataag tatattatta acttaattaa cgacattgag
     7921 gtaaattaat gatgaaagta attttttttg ggttagggtc aattggaaag agacatctaa
     7981 aaaatctaat aagctatgga aaaaagaatt ctattaattt tgatataaca gcttatagta
     8041 gcagtggaaa aaaagagttt gaagaaataa cgtatattca aaatagagac gatttggata
     8101 cacattatga tatagctttt attacaaatc cgacttttaa gcatctggaa acactaaatg
     8161 taattgttga aaaagttgat tacatatttt tagaaaaacc tgctttttct gagagtattg
     8221 atcttactca atataattat gattacaaaa atatttatgt tgctgctcca ctgaggtata
     8281 aaaatatcat ggaagttgtt cgtaatagta ttcaaagtaa aaaagtatat tctgtaagag
     8341 ctatttgttc tacttatctt cctgattgga gaaaaggtac tgattataga aataattatt
     8401 ctgcctttaa atctatgggg ggaggagtag aattagactg tatacatgaa ttggattata
     8461 ttataaacct atttggtttc cctaaatcag taaaaaaaca gtttggtaaa tattcgaatc
     8521 ttgaaattaa cagtaatgat ttagcaacat atatattgag atacgatgat aaaattatag
     8581 aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg tttactttag
     8641 atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa aacattgtta
     8701 aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa
     8761 acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa
     8821 tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg
     8881 ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag
     8941 caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata
     9001 cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag
     9061 aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat
     9121 tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta gatttgactt
     9181 cacctttacg tgaagtttct aatcttgaag aattaataga tgaacatcta aaaaatttag
     9241 ataaagacgt ctacttttca gttgtttctg caagacgtaa tccctatttt aatatggtaa
     9301 aaagaattga aaatgaagtt actatagtta ataaatcaag atacacatct aggcaacaga
     9361 cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa tttctaaaaa
     9421 aaagtaatta tatttttgat gggcaatgtg ggataataaa aatgagagat tatctaattt
     9481 tagatattga tagtgaagaa gattttaaat ggttagaatt tttgtttcct aaatttttag
     9541 aagattcttc ggagcttcaa gatatctata attatttata tcggaggtaa aaggatgagc
     9601 tgttatctta tcaccggtgg ggctggcttc attggttcta ccttagccaa tcatttagga
     9661 gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga aaatcttgat
     9721 atgacgaaaa atatcacttt tattgaggga gatgtcgctg agaaatcctt gatggaagag
     9781 atcatgaaag ctaataaatt tgattatatc tttcatttag cagcagttgc tagtgtggca
     9841 gattcagttg cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt attaatgatt
     9901 ttagaattga tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag ttcttcagcg
     9961 gctgtttatg gggatgaacc gactttacca aaaaaagaag agtccgtgat tcgcccattg
    10021 acgccttatg cggttgataa attcgctgca gaacaatatg tgctagatta ttgtcattta
    10081 tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa tcaaaatccg
    10141 aattctccat acagtggtgt gatttctatt ctcgtagatc gctataagaa acaaatcgta
    10201 ggagaagcaa cttcatttac attatttggt gatggaagcc agtctagaga ctttgtttat
    10261 atagatgatg taatccaagc attactttta gttgcgaatg aggaagctgc actggggaaa
    10321 caattcaatg ttggaacggg taaagcaacc acacttttgg aattgattca tgcaatcaat
    10381 gaaattctcg gtgtagaatt gacacttgaa tacaaagaag agcgttcggg tgatatccat
    10441 gactctctag cagatattac taagatccag tcaattggct atcaaccgaa atatggtatt
    10501 ttaagtggaa tgactaatta tttgaaaact gaaatcaatt aaaacatact aagataagat
    10561 tagagggtaa attcaggctc tttgtcaata aggactgatg agttttgtaa aatcaaatct
    10621 gggatagaa
//
LOCUS       DANKBF010000088        10517 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000088 DANKBF010000000
VERSION     DANKBF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(133..306)
                     /locus_tag="PWY89_002549"
     CDS             complement(133..306)
                     /locus_tag="PWY89_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2067544.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="PWY89_002550"
                     /pseudo
     CDS             754..3116
                     /locus_tag="PWY89_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="PWY89_002551"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="PWY89_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2067545.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="PWY89_002552"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="PWY89_002552"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL2067546.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="PWY89_002553"
     CDS             5704..6489
                     /locus_tag="PWY89_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2067547.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="PWY89_002554"
     CDS             6502..7353
                     /locus_tag="PWY89_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2067548.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="PWY89_002555"
     CDS             complement(7477..7929)
                     /locus_tag="PWY89_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2067549.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="PWY89_002556"
     CDS             complement(8394..8762)
                     /locus_tag="PWY89_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL2067550.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="PWY89_002557"
     CDS             8912..9394
                     /locus_tag="PWY89_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL2067551.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="PWY89_002558"
     CDS             9431..10441
                     /locus_tag="PWY89_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2067552.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DANKBF010000089        10387 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000089 DANKBF010000000
VERSION     DANKBF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(104..346)
                     /gene="acpP"
                     /locus_tag="PWY89_002559"
     CDS             complement(104..346)
                     /gene="acpP"
                     /locus_tag="PWY89_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2067553.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(468..1469)
                     /gene="plsX"
                     /locus_tag="PWY89_002560"
     CDS             complement(468..1469)
                     /gene="plsX"
                     /locus_tag="PWY89_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2067554.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1518..3554)
                     /gene="recG"
                     /locus_tag="PWY89_002561"
     CDS             complement(1518..3554)
                     /gene="recG"
                     /locus_tag="PWY89_002561"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2067555.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3671..5353)
                     /locus_tag="PWY89_002562"
     CDS             complement(3671..5353)
                     /locus_tag="PWY89_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL2067556.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5405..5767)
                     /locus_tag="PWY89_002563"
     CDS             complement(5405..5767)
                     /locus_tag="PWY89_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2067557.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6037..6225
                     /locus_tag="PWY89_002564"
     CDS             6037..6225
                     /locus_tag="PWY89_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2067558.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6463..9132
                     /locus_tag="PWY89_002565"
     CDS             6463..9132
                     /locus_tag="PWY89_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2067559.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9587..10333)
                     /gene="truA"
                     /locus_tag="PWY89_002566"
     CDS             complement(9587..10333)
                     /gene="truA"
                     /locus_tag="PWY89_002566"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2067560.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2836 a         2237 c         1867 g         3447 t
ORIGIN      
        1 gccagaatta tcagaaaaat gacttttacg tgtcaaaaag attaaaaaaa gaaggataaa
       61 aagctgcatg cagcttttta tccttctttg cttttatttg aaattattta agatggctgc
      121 tgatataatc tactgcgcca ccgactgttt caatttgttc agcgtcttca tctgaaattt
      181 ccgtaccgaa ttcatcttca agctctaaaa cgaattccat aatactaatt gaatcagctt
      241 ttaaatcatc tttgatgttc aattcatccg tcactttatc ggtatctatc tcaaaatgat
      301 ttgaaataat tttcgctact ttgttaaata cttcttcgcg agtcaacttt ttcacctcca
      361 agaatcatac acttgtcaat tacctacacc atggctaatt ttactggcat ttttcaggaa
      421 tgtccacctt tttttcaatg gaacggatcg cttcagatat ccaaatacta ttcttcgctt
      481 ttttcaaatt gttcgactaa ttgtccaact acatctgtct caagcattgt gtggatttgg
      541 cggatcgtat agcgaacagc atcggggcct gttgctccat gagttttaat gactggtgct
      601 ttcaagccaa acaaaacggc accgccatgt ttggaataat ccatttctgc tttcaaagaa
      661 cgtaagccgt cttttaaaag catagcgccc atttttcctt tgatcccttc atttagaata
      721 gcagatttta atagattcat catattcatt gctgttcctt caatagattt caaaacagcg
      781 ttgcctgtaa atccatctgt gaccactaca tcagccacac cattgagtag ctcacgagct
      841 tctacgtttc caataaaatt caagcttgtt tcattctgaa ggagttcata ggcttttttc
      901 gtcacttcac taccttttgt tgcttctgtt ccgttgttta agagtgcgac acgaggacgt
      961 ccgatacctc ggactttacg tgcataaaaa gaacctaaaa taccgtattg caacaaatgt
     1021 tctggtttgt tttctgcgtt agcgccaaga tccagcatat cgaaaccggc accttctttc
     1081 cctactactg gcaaggtaga catcaagcca gggcgttcga tatttttgat acgtcctacg
     1141 atgaacaacc ctgctgctag tagcgcgcct gtattccctg ctgaaaatac agcatctgct
     1201 tcaccgtttt tcactgcttg tgcagcaagg accattgatg cagtcttttt tcgtcgaatt
     1261 gcttttactg gctcatcatc actattgatt ttttcgtctg tatggataat cgtgatgttt
     1321 ttttcatttg ttaagtattt cttgatttct gcttcttttc cgtaaagtaa gaattctact
     1381 tctggaaaat cttgctgcgc taaagccacg ccttctacaa ttgcttgagg tgcgtgatct
     1441 ccgcccattg catctacggc aattttcatt gtttccactc ctttttccgt catcagtata
     1501 aagtatagca cagtctgcta atcgaaataa cgattatcct gattttctat ttggaggctt
     1561 tgcgctaaag gctgatactc aggaagcatc tgccaatcct ttatttgcca aagggctgca
     1621 gcctcttctc tggctacctc caaaacattt ccatccgtca ccaagtcagc aatcgcaaat
     1681 tgaggcagac cagattgacg gaagccaaat acttcaccag gaccacgtaa ttccagatct
     1741 ttttcgctta gtacaaaacc attggtggtc tctgtcatga ttttcatccg ttccacacct
     1801 agttcatttt taggattagc aactaagata cagtaggaag catcacttcc gcgtccaaca
     1861 cgtccacgaa gttggtgcag ctgtgccaat ccaaatcgat ccgcatccat gatcagcatg
     1921 acggtcgcgt tcggcacgtt tacccctact tcaattacag tagtcgaaac taatatctgc
     1981 atctggttgt ctttgaaggc ttccatgatt acttcttttt cctgattctt cattttacca
     2041 tgtaacaagc caacttcata ttttggtgca aatagttcac gaagtttttc atagatttcc
     2101 actgcatttt ttacatctaa tgcttctgat tcttcaatca gcggacagat gacatacatt
     2161 tgatgtcctc ttgccagttc ttttttcgtc caatccagta cactgtccaa ttgaggtgtt
     2221 cttacccagc gtgtttcgat cgggatacgt ccagctggaa gttcatcaat gatagataca
     2281 tccatctcac catatgctgt aatagctaat gttctaggaa tcggagtcgc tgtcataaat
     2341 aagacatctg gtcgccagcc tttttcacga agtactttcc gctggttcac gccaaaacgg
     2401 tgctgctcat ccgtaataac tagacctaaa ttctgaaatt cgactccatc ttggatcaat
     2461 gcatgtgtgc cgatcacgac gttaatttcc ccttgctcta gtttttcaag gagctcacgt
     2521 cgttcttttg ctttcgttga ccccgtaatc aatgcgatgg ttacttcatt gggatcaaac
     2581 agctgttgca agctttccat atgttgttgt gccaagattt ctgtaggcac catcaacgca
     2641 ccttggaaac ctgctgtaac ggtagcgtat aacgcaatcg cggcaaccac tgtttttccg
     2701 ctacctacat ctccctgcaa aagtcgctgc atatgctgcg gactgcgaag atcccgacag
     2761 atttcattcg ttacacgttt ttgtgcattt gtcagttcaa aaggtagctt ttgagtaaat
     2821 tgtttcaatt tttggacatc ataaaggatc tccaaaccat tcgtttctgc tttttctgct
     2881 tttttcaatc cttgaagacg catttgaaat aaaaagaact cttcaaaaac gacacgtcgt
     2941 ttggcttgat ggttttcttc gtggtctttt ggaaaatgca tacttcttac agcagaagct
     3001 cggtcaagca gtcgatattt ctccacaagg ttgtttggca gattttcttc aatcaatgtg
     3061 ccgtattctt gaaacccttt tttgattaga tcaactaatg tcgtttgacg aattgatttg
     3121 ttgacgtgat atatcggcga aaagtcatcc attgtttgag aacctaaaat cttcatcccg
     3181 ttcaaggctt tacgctttgc atcccattta ccgtatacgg cgatttcctc cgataaaatg
     3241 actttttctt ttagataagg ttgattaaaa aaagaaacgt tgaacacatc atgttcttgc
     3301 atcatacgaa attgcaatcg gcttttttta taaccaaatc gattcatcac aggaggcgag
     3361 actacgattc ctttcaaagt aaccttttct tgatcctgga tctcgtttag atttctttcc
     3421 tgaatatctt cgtagcgaaa aggataataa gtcagtaagt cttcaatcgt ttggatgccc
     3481 aagctggcaa gactagctgc tcttttttct ccgactcctg ccagcatagt gattggatca
     3541 tctaaagaac gcataaattt ttcctccctc ctttaacaaa aaaaggactt ggtcagcgat
     3601 catacctgat cactaaccga aggcctttta tgaatgaata ctgtgttaga aagatattct
     3661 gacacagaaa ttattcagca gaaaatagat aaggatagac aggctgatct ccttggtgga
     3721 tctcgatttc caaatcagga tgtttttctg ttagtttctc agcaaattca tttgcttgtt
     3781 caactgttcc ttcatcacct gccaagatcg ttacgatctc tgtgtcctca ttgatcattt
     3841 gttctagtgt acttaacgct gttgctgtga tatctgggtt agagatcagg atttttccat
     3901 cgatcatgcc caaaaagtct ccttctttga tctctacacc gtcaattgag gtatctctga
     3961 tcgcgtgagt gacttgaccg ctcacaacgc tagacaacat ttctgtcatc gtttctttgt
     4021 tttcttctaa agaagcttga tcattgaatg aaagcatagc tgtcaatcct tgggaaatcg
     4081 ttcttgaagg aacaactgct actgggatat ctgctacttc tgctgcttga tcagctgcca
     4141 tgaagatgtt cttattgttt ggcaagacaa tcactttatc tgcgtttact tcttgaatcg
     4201 ctttaacaat gtcttccgta cttggattca ttgtttgtcc accactgata acataattag
     4261 cgcctaaact ttcaaacagc tcttggacgc ctttaccagc agcgatcgct acgataccat
     4321 acgggatacg tggtatttct tttttcgcaa actcgttcac ttcttcatcg tgttccaaaa
     4381 tcgtctcatg ctgcaagcgc atgttgtcga ctttgatttt gactaatgaa ccgaattttt
     4441 gtccataatt caagacttct cctgggtttt ctgtatgcac gtggactttg atgatctcgt
     4501 catcattgac cacaagtaaa gaatctccta taccatctaa atagttacgg aattcttcgt
     4561 aatcgaatgt actatcgact gtaggtcctt ctccgatttt taccatgatt tctgtacagt
     4621 aaccaaaatg gatatcttct gtcgccattt gaccttggac actgcgatga tgttctgcat
     4681 tgaccatatc atccatttct gctggagaag gttcatatgt ttcatccgct tggaattctc
     4741 cattcaatgc acttaaaaag ccctcataaa caaacagcag tccttgccct ccactgtcta
     4801 cgacaccgac ttcttttagg actggaagca agtctggagt tttagctaaa gcacgctttg
     4861 ctccttttac aacagctgtc attacttcaa cgacatcatc agtacttttt gctttctttt
     4921 ctccagcttt cgctgcttct ctagctactg tcaaaatcgt tccttctact ggtttcatta
     4981 ctgctttata cgcagtttca actccatgag tgaaggctgc agcaagatca gaagcattta
     5041 atgagacaac atctggaatt tgcttagaaa atccacgaaa caattgagac aaaataacgc
     5101 cggaatttcc gcgcgcaccc atcagcaatc cttttgaaag cattgctgct aattctccta
     5161 ctttttctga ggttgaatct actactgcct tagctccact agtcatagat agattcatat
     5221 ttgttcccgt atcgccatct ggcacaggga atacatttaa tgaattgacg tattctgcat
     5281 ttacttgcag acgattcgca cctgcctgga ccatttcctg gaactggctt gcacttattt
     5341 ctgttacact cacctagata atcctccttc agatcgactg ctgagttacc agccagtctc
     5401 ctgcctagtc tggcagtaca cgaacacctt gtacaaagac attaactgaa ttggcagtta
     5461 cgcctaataa tgcctctaga ttgtatttca ctttttcttg gacgttgcgg gaaacttcag
     5521 agattttcgt accatagctg ataatcgtat aaacatctac agcaatacca ttatcttctt
     5581 ggcggacaac tactcctctt gagtaatttt ctttacgcaa aatctcagta atattatctt
     5641 taatttgatt tttgctggcc attcctacaa caccgaacac atctgtcgcg gcgccaccaa
     5701 caactgttgc aattacatca ttggtaattt caatggttcc agctgacgtt tgaattttta
     5761 cagccatgaa tgagcctcct taacgggtaa aaaactccca tttttcttct atcatatttt
     5821 atcatagcgc aacttgaaat aaaaggcagt tacccaaagt actcaaaaac cttgcaaaat
     5881 ttcaggtata accttgttta atttacaatt attcttctca attgtcaatt gtatctatga
     5941 aaatgtactt gcaaattccc attctatatg atatgttagt ctggtatgag ctgaattatt
     6001 ctattgctcc aaatcaaggc aaaggaggaa attaccatgg caaaagtttg ttactttaca
     6061 ggtcgcaaga caaaaagtgg aaacaaccgt tcacacgcta tgaactcaac aaaacgtact
     6121 gttaaaccta acttacaaaa agttcgcgtt atggtcgacg gaaaacctaa aaaagtttgg
     6181 gtgtcaactc gtgctttgaa atcaggaaaa gttgaacgcg tttaattgaa tgatacttgg
     6241 accgttcaga tagtgaacgg ttttttattt aataaaaatt tttaagggtg ggacttttga
     6301 ttcctaccct ttttttgcgt ttatttttgt tatacgaaca aattatgaag ttatttattc
     6361 taaattttat ttttttgtga aaactagact ctttttaagc agagaaaaac aaggatatga
     6421 taaactgaat acaacttcat agaaatttgg tggaaaataa atatgaaaaa aaatttaaag
     6481 tcattcttta gagaaaacgg acgctacgca gcagctagtt tcctgattcc gtttttgatt
     6541 ttagcacttg tttatctaag tatcggaatc tatcctggaa gcagcagaag tattttggca
     6601 agtgatgcct tttcacagtt ttctaatttt catgccagct tccgaaatgt tcttcttgga
     6661 aagcaaagta ttttttatac atggaatgct tctctaggat tgaattattt atctttaatt
     6721 tcttactatc taggcgggat cttcacgcca ctggtgttct tttttccaaa ccagatgatg
     6781 ccagatgcac tgtatgttct gactttgctt aaggtcggct gtgccggatt ggctttttgg
     6841 tttcttgcac gtacgttcaa gattcctcgc tgggggcatg ttgcacttag cgtcagttac
     6901 gcattgatct catttatcac tgcacattcg gagatcatca tgtggctgga tgcatttttt
     6961 tatcttccat tagtgatttt ggggattcat cgtgttatgg atcttaaacg gccgaaactt
     7021 ttatttatca gttatttact cttgttttta tccagttttt atatgggttt tatgattggc
     7081 gtattttctt tcttatattt tatcgcccgt ctattgacaa actggtcaga atataaaaaa
     7141 acgattgttc cttatggtat cacttcttta ctggcaggcg gcgcttcaat gatcattgta
     7201 ttgccagcag tgctggatct tcggtcaaat ggcgaggcac tgagtcagat cactacgttg
     7261 aaaacagaag cgacttctta tctggatcta atcatgaaaa atatgattgg tgtttatgac
     7321 acgacaaaat acggatcgat tccgttcgtt tacgcaggtc tgctaccatt agtcctctgt
     7381 ctgttttatt ttgccagtcg gaaagtaccg ttgaaaaata aatttttgta cggcagtctt
     7441 ttcgccatac ttatcgcaag tttttatctt gttccattga atttgttctg gcatggtatg
     7501 catgcaccga atatgttttt attccgttat gcctatctgt tctctttctt agtgctcctg
     7561 cttgcaggaa aaggatggga aaagatagaa caagacgatc gtgggctgtt gttaggaatc
     7621 attttggtcg tagctgctct ctttgcggtg gcatggggca tcaaacctga aggaagctac
     7681 acctatgtga cgatgacttc ctttgtattg acagtggttt tcttagggat ttatgctttt
     7741 gctgtgggat tttatcagca taaaaaactt tcattcaatc atctgagtat attgttatta
     7801 ttgatcatgt ctgctgaagc actggtcaat acgaacagta tgatccatgg cattcttgat
     7861 gattggaatt atgcttcccg cagtttatat actgatcctt atcctgactt gaaagctctt
     7921 gtaaatgaaa caaaaaaaga ttccgactcg ttttaccgct tggaaaatct ggatccagta
     7981 tctgcaaacg attcgatcaa ctatggttac agtggtatca gtttgttttc ttctataaga
     8041 aatcgtaatt cttcctctta cctggataaa ttaggtttcc gttcaagagg gacaaatttg
     8101 aatatccgtt ataacaataa tactttgctg atggatgggt ttacaggaat caaatataac
     8161 atttctaaaa acgaaagcac gttcagtaaa tatggcttcc gccaagctgg tcaaagcggc
     8221 gactatacac tgtatcagaa tgtcaatgca ttgccactag catttactgc accgttgtcc
     8281 gttaatgatg ttgagcagcc agtgacagat aatttgacca gtcagacaaa cttgatcaat
     8341 acattgtctg gtctaaatga acagtatttt acattctata cacctacttt gaagaatcag
     8401 ttgaatacga cgatcacaaa gacaacgact ggcgtcactt actcagaaac agcagataac
     8461 cagccaaaag agctcacttg ggaagtccat gtgcctgcaa atacacaagc ttacttaagt
     8521 ttgttcccaa ccaattttgc gcaattagaa agttctagtg cgacgatcac tgtcaatggt
     8581 acatcgcgaa aaacgcaaat caacatttct ggtcaatact atgatcttgg ctattatcct
     8641 caagagacaa cagtgacatt tactgcaagt ttttatggga cgaaagaaat aagtttcatg
     8701 gagcctaaag tcgttggtct agatgttgta gcctatcaag ccgcaatgaa tcagatcaaa
     8761 gaaaaaggtg tcgaaatgga agccactggc agaaaagctt ctgggactgt aaataccgct
     8821 gaagattcca tgttggtgac aactattcct tatgacaaag gatggaaagc taaaattgat
     8881 gggaaaccag tcaaggtcag caatttcaaa gacgcattct tgatgataaa agttccagca
     8941 ggtaaacata ctgtcacttt cagttacttg cctgaaggat ttacgatagg tgcaatcctt
     9001 tttgtcctat gtattgcatt gtttattctt tatgtctggt accttgaccg caatcgcccc
     9061 ttttatggca cgattgatcc agcaatagaa gaacaaccac gaaacacaca cagaagaaga
     9121 cgtagaaaat aaagaaacga tggaacctgt tcagactgtc cgcaccaagg gcaatcaaaa
     9181 caacgttcca tcgttttttg gttacagaaa aaataggtaa caaaaagatg attcacttat
     9241 caacgcctcg tccacaagtc acgatattca tcaaagatga gaagctgttt tttgaaattg
     9301 ctctttgtga ctcagagtta aatgacaggc tcacgacctc tttcttaaac ggtgttcaat
     9361 cagctgacct tcggtattag ctcaacaaac ggcaaaatat gaggagctat tttcccgttt
     9421 gtttctctaa tactcaggtc aaaacgctga tttcacgacc tcttccttaa acggtgttcc
     9481 agaagtagct tcttcgaaaa taagccgaaa ttttgcaaaa ttttgagaag taattttcgt
     9541 aaataccttc ttatttcttg aagctgacca cttctatcat aacctcttat tcatatttca
     9601 cttcatacaa ataaagtcct tcagggtgtg ctgtaggtcc tgctgcatta cgatcttttt
     9661 tcgcaataat atctgggata ctatcttcag gcaatcggcc attgccaatt ttcaaaagcg
     9721 ttcctactaa gatccgtatc atcttataca agaagccatt tcccctaaat gtaaacaaca
     9781 attcgtcttt tgtttcattt acctccatct tagcctcgta gatcgtacgt attttatcct
     9841 ctatcgagct tcctgatgca caaaaagagg tgaaatcgtg agtccctaat aaatcaggaa
     9901 gcgcccgctg aattttttcc aaatcaagtg ggtaaggaaa ataacttgca taatggcgtc
     9961 gaaaaggact gcgtggtttc cctatatcca ctcgaaattg atacgtcttt tctttgacca
    10021 aatatcttgc gtggaagtct tctgaaacaa tttctacttg tcttacagca atatcctctg
    10081 gtgactgggt atctagcgcg aaacgcattc gttctaacgg acgctcctct ggataatcaa
    10141 aatggattac ttgcccgatc gcatgtactc cggcatctgt ccttcccgaa ccaaagaccg
    10201 tgatttcttt tccatttgcc attttttgta atgttttttc tacttcttct tgaacagtac
    10261 gcccatttgg ctgtttttgg aatccattaa aatttgttcc gtcgtacgca atgattgctt
    10321 tgtaacgtac catgtttttc tctcctttgt tcctaaaagg ttatctggaa taatgatttt
    10381 tctataa
//
LOCUS       DANKBF010000090        10248 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000090 DANKBF010000000
VERSION     DANKBF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            162..311
                     /locus_tag="PWY89_002567"
     CDS             162..311
                     /locus_tag="PWY89_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL2067561.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            322..1836
                     /gene="dltA"
                     /locus_tag="PWY89_002568"
     CDS             322..1836
                     /gene="dltA"
                     /locus_tag="PWY89_002568"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL2067562.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1833..3038
                     /gene="dltB"
                     /locus_tag="PWY89_002569"
     CDS             1833..3038
                     /gene="dltB"
                     /locus_tag="PWY89_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL2067563.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3083..3316
                     /gene="dltC"
                     /locus_tag="PWY89_002570"
     CDS             3083..3316
                     /gene="dltC"
                     /locus_tag="PWY89_002570"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL2067564.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3319..4587
                     /gene="dltD"
                     /locus_tag="PWY89_002571"
     CDS             3319..4587
                     /gene="dltD"
                     /locus_tag="PWY89_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL2067565.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4728..5810
                     /locus_tag="PWY89_002572"
     CDS             4728..5810
                     /locus_tag="PWY89_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL2067566.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5810..6613
                     /locus_tag="PWY89_002573"
     CDS             5810..6613
                     /locus_tag="PWY89_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2067567.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6683..7423
                     /locus_tag="PWY89_002574"
     CDS             6683..7423
                     /locus_tag="PWY89_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2067568.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7434..8438
                     /locus_tag="PWY89_002575"
     CDS             7434..8438
                     /locus_tag="PWY89_002575"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL2067569.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8483..10180)
                     /locus_tag="PWY89_002576"
     CDS             complement(8483..10180)
                     /locus_tag="PWY89_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067570.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3290 a         1697 c         2081 g         3180 t
ORIGIN      
        1 cgagaaatta taaagaaaaa agcagtgatt tgatgtgcta tttttataaa agctatgtga
       61 caaaactgtt agtaaaaaaa cggtttctgt aagctagaca catgtttctt tttgtcaaaa
      121 ttagttacac tttgtgtgag gttatatgag gagggaatcc aatgaacaaa tggcgttcta
      181 atccaaaatt gaattattgg ggaaaattca tcattcgaac aattttttat agtgcagtac
      241 tgctgttcct gatttatctg tatcactata aaaatattca aggaggaact tttatctata
      301 acgaatttta ggaggaagag tatggaaatc aaaacgatta ttgaagcaat cgatgattgg
      361 ggaataaatg aacctgatcg agttgcttat caggcggatg agacgcatac ttttggtgaa
      421 ttgaaacaag catcagacgc acttgcttat tatttagaaa aaaaggtaga aggcgatgga
      481 ccaattgtcg tttttggcaa tttggagttt gaaatgattg tctcatttct aggggttgtc
      541 aaatctggcc atgcctattt gccgattgaa gagcatacac ccaaggagcg tatcctatcg
      601 atctttcggg tggctaaacc atctatggtc atcagtatcg gggactggat agaagatatt
      661 ccttcggttc ctgtcataac aaaaaaagaa tttcaagaaa tcaagaagat accagtagga
      721 ttttatccca gaaatagcgt aaaaggtaca gaaaattttt atattatttt tacttctggt
      781 actactggag aaccaaaagg cgtacaaatc agtcatgata atctagtcag ctttgttcaa
      841 tggacgatgg aagactttgg gatcaagcct ggtatgcatt tcttagccca agcgccattt
      901 tcttttgatt tatcggtatt ttctatttat cctgcattag tttctggcgg aatgctgaag
      961 ccgttaaata aagcagttgt tcaagacttt cgtcaattat tcgcgacatt gccagccttg
     1021 aagttgaatg tgtgggtttc tactccatca tttatggata tttgcttgat ggaacctaca
     1081 ttcaatggag aaaatgtgcc ggaattggac atgtttttat tttgcggaga agaattgacg
     1141 aaaaaaactg cagaaagtct acttgatcga ttcccaaatg cacggatata taatacttac
     1201 ggtccaacag aagcaactgt agctatttca agtattcaga ttgaccagca tgtactagat
     1261 agttatgacc gactaccgat tggctacgta aaaaaagaca cgaaagtttc tattgtccaa
     1321 gaaggtaaac ctgtttcaaa aggagaaacg ggtgaaatca ttattgcagg accaagcgta
     1381 tcaaaaggtt acctgaacaa tcccgaaaag acagctgctg cgttcttttg tcaaaatgac
     1441 ttagcaagtt accatacagg ggatgcaggt agactagatg aatatgggct tttattttat
     1501 gaaggacgta tggatcagca ggtcaaatta catggatacc ggattgaact cggggatatc
     1561 gaacattatc tgctccaaga caaccgaatc aaacaagctg tcgtcgtacc aaaataccaa
     1621 ggaacaaaag tacagcagct ggttgctttt gtcgtattag aaaacaaaac gaaacagccg
     1681 gattttcagt tgactaaatc tatcaaagaa caattactag aagttgtaat ggattatatg
     1741 attcctcaga agttccatta tgtcgatctg ctgcctcaga cagttaatgg aaaaatcgat
     1801 cgaaaaaaat taacagctga ggtgaatcct gaatgatgtt acctcatatg atcccttatg
     1861 cagagccgtt ttattttttt cttttagcca ttgcgcttgt accgatcgtt ctatctttat
     1921 tgatatggga aaaaagatta ccagtttatc aatcagcagt gacacttttc tttttatttg
     1981 tcagctttgg aggtaattat tggcaacagg gagcggcgct gattgggtat gttttgtggc
     2041 agacgatact tgtatggggc tattttgttt atcggcaaag gaaaaatcag acaggatggt
     2101 tttttgccgc cgttatttta gcgataattc cactgttttt aacgaaagtg actccttttt
     2161 ttgaacatgg aaaagcatcg ttacttggtt ttttaggaat ctcttattta acctttaaat
     2221 ctgtacaaat gatcatggaa ataagagatg gcatgatcaa aacatataag ccatttcgtt
     2281 atatccagtt tctgctgttc tttccaacaa tctcttctgg accaatcgat cgctatagga
     2341 gatttgaaaa agatcagctc catccgcctg ttcgtgataa atatgttgat cttttgggaa
     2401 aaggcgtcca aaatattttt gtcggttttt tatataaatt cattatcggt tattattttg
     2461 gtcaagtact gcttcctgta gtgggacgca ttgccttaca gcatggtggt atttcatggg
     2521 cattagtagg ttatatgtac gtgtacagta tgtatttgtt ttttgatttt gctggctata
     2581 gcttatttgc agtcggtaca agctacatga tgggatatca gacaccaatc aatttcaata
     2641 aacctttttt atcatggaat atcaaagaat tctggaatcg ttggcatatg acgctgtcct
     2701 tttggttccg tgattatgta tatatgcgat tgatgttctt catgatgaag aaaaaatttt
     2761 ttaaaagcaa gatcgtaatg tcaaatatcg gttattttgc tttgtttctt ctaatgggta
     2821 tatggcatgg attgacgtgg tactacatcg tatatggact gtatcatgct tgtctgatct
     2881 gtctgacaga tgcatggatc cgatttaaga agaagcataa agagcagttg ccttctaaca
     2941 aatttacgca tggcttagcc gtatttttga catttcaagc agtatgtttt agcttcctga
     3001 ttttttcagg atttttagat aaactttttt ttagctaacg aatagtgaca tattaattta
     3061 tataacaaag gagaaaagga aaatggaaga acaaatttta tcaatcttag aagaaataac
     3121 aggaacagat gaagtaagaa aaaatcgtga tattgatcta tttgatgaag ggttgcttga
     3181 ttctttagca tctgttcaat tattagtcga gctagacgga gagctggata tccaagtccc
     3241 tgtatctgaa ttcgagcgag aggattggtg cacaccgaat aaaatcattc aacaagcaac
     3301 tgcattgaag ggttgagtat gaaaaagaaa ctttttgcta ttttcggccc ggttgtttta
     3361 gctgttggct tactgtttgt gttttttacc tcctcctatc aagtagatat ctctaatcca
     3421 gaaacgatta gacgtgcctc ttcttccatg tcagtgaacg tgttgaaagg agatgcgata
     3481 aaaaattatg cattatctga aaaacaatat attccttttt ttggctcaag tgaattaagc
     3541 agaatcagtc catttcatcc gtctgttctg gcagaaaaat accagcgaaa ctatcgtcca
     3601 tttttgctgg gagcacccgg gacacaatcg ttaagccaat atatgatgat gcgttctgca
     3661 ggagatgcta tgaaaaacaa gaaagtcgtc tttattattt ctcctcagtg gttcgttaaa
     3721 aatggtgtaa aaactgatta ctttaatacg tattattctg aacttcaaac atacgactgg
     3781 ttgttttcaa tgaagaaagt aacgcctgct gatcgctatt tagctagaag attattaact
     3841 ttctcaaaag taaaagagaa tgatacgttg acggcgattc tgcaaacgat caaaaaaggg
     3901 aagctacctt tacctgaatc tttgaatcag ctgcgcagcc aatggaacat gctgaagaga
     3961 gaggatgaag tattcagcaa tattgggttg aaagatcgac agcaaaaaat cgatcatgag
     4021 tcaaaacgat taccaaaaca ataccaagaa acagaattat ccattttagc aaatcaaatt
     4081 ggagaacgag aaacgacgaa taatccattc gggttgaaaa acgattttta tacgcatcgt
     4141 atccgagccc acgagccaga gctcaaacag tcacaaaaaa attgggatta tcgtttttcg
     4201 ccagaatttt ctgattttca gctggtcttg gatcaattag caaaaaacca taacgaagta
     4261 ttgttcatca tcccaccggt taacgagaaa tggagcgatt ataccggact atctcaagaa
     4321 atgttacaag gatttgccaa aaagatcaaa tttcaattaa atagccaagg attcaatcga
     4381 atagctgatt ttgtcaatca agcgggtacc aactatttta tggaagacac gatccatcta
     4441 ggctggaaag gctggttggc tgcagatcaa cagatcagac cgtttttaga agaaaatcat
     4501 attactgcta gtaagtatca tcttgacgat gcttttttta gcaaatcttg gcaacatcaa
     4561 ataccagata aactacaatt aaaataaaga aaacaggcga gtgttgctcg cttgttttct
     4621 ttatttttga atattctgaa taaaagataa aaataccaag tttgcctgaa caaaaatcat
     4681 aagcaatggt ataatcacaa tatgaatata gaaatggggg aaaacatttg gatacaaaag
     4741 aagaacagct acttattgat ttaacttctg caaaaggagt acctggaaat gaagaagagg
     4801 taagagaagt attcagagaa tatgccaaac cattcgctga tgatattttt tatgacggtt
     4861 taggcagtgt gattgccaaa catggtgctg gcggaggccc caaggtcttt atttctggac
     4921 atatggacga agttggtttt atggtaacaa aaataaccga aaaaggattc ttggaattcc
     4981 aaactcttgg aggttggtgg gggcaagtca tgcttgctca gcaagttgaa atcaaaacac
     5041 gtgaagggaa aatcgtccac ggtgtgattg gttccaaacc accacatgtt ttaacacctc
     5101 aagtaagaaa taaaccttat gaaataaagg atatgttcat tgatattggc gcatcaagcc
     5161 aagaagaagc caaagagtgg ggcatacgtc ctggagatat ggtcactcca tatattgaat
     5221 acaaacgaat gaatggcagt aaatatttac tggcaaaagc atgggataat cgtatcggga
     5281 cagctgtttc tctgcgagta ttagagaatc tatctaaaga agcacaccca aatgttttat
     5341 tcgcaggaag cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca agtacacacc
     5401 ttgtaaatcc cgatattgct tttgctttag atactggtac agcaggagac actccaggaa
     5461 tgacaccaaa agaagcagat tcgattttag gaaaaggccc acagattttg atttttgatg
     5521 catctatgat cccgcataaa aaattattga attttgttat tgatgtagca gaggaaatgg
     5581 atattccatt ccaatataca gtgatttctg gcggaggaac tgatgcagga caaatgcatt
     5641 tgacaagaga tggtgttccg tctgtagcca ttaccgttcc tgttcgttat ctgcattccc
     5701 atacatcagt gattcatgaa gatgattatt tgaatacagt gaaactagtc acagaattag
     5761 tgaaacggct tgatagtgaa acagttgaaa aaattcggag ttatgaataa tgaataatag
     5821 aatacctatt tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc aatggggaat
     5881 caatacttgg cttgttttct tatctataat atttacgagc gtcatccaga acttttttga
     5941 cggtttcagt ccttttccgg aagaaagcat gcagcagcac ttattgagtt ttctagtcga
     6001 taatttactt ttattcgcat tcaactatgc attgttttac attgcgctag tagttgtacg
     6061 gggtggcaga ccaaaaagta accttttatt ttcagttttc caaagaaagt attttggttc
     6121 gatacttggc atcaatttga tcaattcagt catcaactgg ctactcaaca gtttaatctt
     6181 tctgcccatg tttttaatca gcggagtgag tacttatatg cagctgcttt tcagttcaaa
     6241 tgcggtacct acgggtgtca tgccagaaga ttttcttgat atcagctttg ttttatcgat
     6301 gatagggatg atttttgttt cagcactcat catgagtatt gttggcggtt tattccaatt
     6361 tgctgcatgg acaaagttcg actatcctga tttgtcggtt attcaatgtt tgaaatatgg
     6421 ctggttttta ttgaaagata gaattggtac gtatattttg ttgcagctgt cttttatcgg
     6481 ctggtatttg ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt
     6541 gaatgtgtca gttgcagaat tttatgaaca agcacggatt gaaaaaggag gtccagacgc
     6601 ctattttact tagcatgtaa gcagtttatt catcaaaaaa ataacttttg tggtaggata
     6661 aatctttgag ggggaatgta cgatgaaatt aactgtttta ggatgcttag gagcgtatcc
     6721 ttataaaaat caagggacaa caggttattt gcttcaatct gacgggttca atttgttgat
     6781 cgatgcaggt agtgctacgc tgatcaaatt acaagaacac cttgatccac ttgatctaga
     6841 tgctgtgatc cttagccatt atcatcatga tcatattgct gaccttggtg tattacagta
     6901 ttactggcaa cttcatccta agagaaatcc ggagacattg ctttcaattt atggacacac
     6961 aaaagattcc attcactttg aagaattgac gttggaaaat gtctcaaaag gacatcctta
     7021 ttttgaagct gaagaattaa aaattggtcc ttttctgatt acttttatgg aaacaatcca
     7081 tccagtagta tgttacgcca tgcgtatcgt agaagaaaca acggggaaag tattcgtatt
     7141 tacaggtgat tcaggttatt tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt
     7201 tctagcagat acctatcttt ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa
     7261 agaagctgga gaacttgcga aatcagctaa cgtaaagaaa cttgtactta cacacctacc
     7321 tcagtttggt gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat
     7381 tgatatagaa cttgctgcag ttgacaaagt gtttaacatt taggaggaag aacatgattt
     7441 ttgtaccaaa tgaaaaccac gacccaagaa tcaatttagc aattgaacaa tatttgctgc
     7501 aagaaatgcc tttagatgaa cctattttgc tcttttatat caatgaacct tcaatcatca
     7561 tcggcagaaa ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca
     7621 ttcatgttgt ccgtcgctta agtggaggcg gagctgtcta ccatgatttt ggaaacctga
     7681 actttagttt tatcatgcct gatgatgggg attcattccg tgactttgcg aaagtaacac
     7741 agccaatcat ccaagcactt catgaattgg gtgttgctgg ggcggaattg aaaggccgaa
     7801 atgatctagt cattgatggc atgaaatttt ctggcaacgc gatgtatgct accaacggtc
     7861 ggatgtttgc tcatggaaca attatgttcg actctgatat caatgaagta gtcaatgcat
     7921 taaaagtaaa aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa
     7981 cgaatatcaa accatttctt ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc
     8041 aagaaatctt gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat
     8101 tgactgatga agattggaaa aaaatcaatc gaatctctga ggaatattat cgaaattggg
     8161 attggaatta cgggaagtct ccagatttca atttctctcg ccaaaaacgt ttttctatcg
     8221 gttcgattga agtccatttg aatgtctcag aaggaatgat caaagaagca aaaatctttg
     8281 gcgatttctt cggactagga gatatctctg acgtggaaaa aaaattggtt ggacagaaat
     8341 atgataaagc atctctatca aaaatagtca gccaaattga tgtgaagaaa tattttggtg
     8401 cgatagaacc agaagaattg cttaccttac tttattgaaa ataaaaaata gcggaatcca
     8461 catatatgga ttccgctatt ttttatacag gattcgggta atttcgagag agttgaacag
     8521 tgatccgatc aatgaaagta tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta
     8581 cgtctttcat catcattaag tcaaaacgtt cctgatcatc taattcttct gtgatgcctc
     8641 gactaaatag gtttgtatcg taatgtgcac tatgttcgca acaaatgacg aaatgttcct
     8701 cttgttcgtg tatcgacaaa taaatttttc gatacagtgg attttcgatg gcatgagttt
     8761 ctctaaagga atgctctaaa gcgtggatca aaagttgagc cagatcgtca tcgttgatag
     8821 gaagttcttc gggaagatct gcttggaagg ttatctgaat attccgtttg gcagctaact
     8881 tctggtaacg cgctaagata agatttgtca cctggtggtt gcaaaaagaa cacagcattt
     8941 cgaaattttt tgcttcttct aatatctttt ccacatagtt tttagctttt gtttggtccg
     9001 tatgcattaa atcttgcaga ttctccatct cttggacaaa ttccaggcga agtgtattta
     9061 cttcttggat atgctgtcga agctgctcat aatgttgctc caacaaatca tttttgattt
     9121 ttaagaaatc tacttgtctc cgatctttgt cggtatttaa gatgtgatcg attagattgt
     9181 agctgataat aacgattaga atgataatat ataaaatcgt agaccaattg atcgttgttt
     9241 gatagatacc taatgccgtt tggatgtaaa tcaagcaatg gataatggct accaagacga
     9301 tccaaggtgt acaagctaca taaaaacggt tattcttata tgcttcgcca atgcatgcaa
     9361 gtgaagtgac taaagtgctt ataacactag caatattagc aatcggcatt aattcaggaa
     9421 gttctgcagg agtaaaagtt gcataaagca gaacgccgat aaaaatcaaa agttggaacg
     9481 tacaaaaaat tccgtaccat tttttatagt aaaacattcg gctccaatag tagcagatga
     9541 tgaaagctga cgttaagata ataaataaat gagataaaaa agaatgaacg aatggttcaa
     9601 aaagaatacc acttaaaata tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc
     9661 aacttaagag aataagtgag tattcaagac gtttttgttt atacatcaag tagatcgtat
     9721 agaaaaaggt ccctatagcg ataaaaacag cgattatcat cgtcaatact tgcggcactg
     9781 attgtgaata aataaaacta atcaatggtc cagttgttcc tagataaact ttcggtggca
     9841 gtccagcata gttctgataa ggagatgaga ctgtaagggt caattttttt ccgatataat
     9901 ttgctggtaa atctatcaca gccaatgaga gtccgggatt gctttcttcc tgataatctt
     9961 tccgttggta taaaatcttt tcgcctagat atacagtaat ccattgatgg tttcctttga
    10021 ttagtaaatt tgcttcgtcc aacggttttt caagtgtcgt ttccataatg aatgtttcgt
    10081 ctttatccac ggtaggaatc gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat
    10141 cttttgtata gaaaacccac gaatctttta tttctttcat ttgattgctg tccgcaggag
    10201 aaaaaataaa ataaaagaaa taagcaattc ctgttaaaaa taacaatg
//
LOCUS       DANKBF010000091         9820 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000091 DANKBF010000000
VERSION     DANKBF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..195)
                     /locus_tag="PWY89_002577"
     CDS             complement(<1..195)
                     /locus_tag="PWY89_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067571.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="PWY89_002578"
     CDS             complement(211..825)
                     /locus_tag="PWY89_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL2067572.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="PWY89_002579"
     CDS             complement(972..1655)
                     /locus_tag="PWY89_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2067573.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="PWY89_002580"
     CDS             complement(1764..2222)
                     /locus_tag="PWY89_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2067574.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="PWY89_002581"
     CDS             complement(2306..4069)
                     /locus_tag="PWY89_002581"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2067575.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="PWY89_002582"
     CDS             complement(4290..5750)
                     /locus_tag="PWY89_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067576.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="PWY89_002583"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="PWY89_002583"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL2067577.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="PWY89_002584"
     CDS             complement(6907..8334)
                     /locus_tag="PWY89_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2067578.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="PWY89_002585"
     CDS             complement(8365..9579)
                     /locus_tag="PWY89_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2067579.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2913 a         2041 c         1627 g         3239 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaataaa
//
LOCUS       DANKBF010000092         9813 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000092 DANKBF010000000
VERSION     DANKBF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(34..2154)
                     /locus_tag="PWY89_002586"
     CDS             complement(34..2154)
                     /locus_tag="PWY89_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2067580.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="PWY89_002587"
     CDS             2384..3562
                     /locus_tag="PWY89_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL2067581.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="PWY89_002588"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="PWY89_002588"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL2067582.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="PWY89_002589"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="PWY89_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL2067583.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="PWY89_002590"
     CDS             complement(4915..6168)
                     /locus_tag="PWY89_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2067584.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="PWY89_002591"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="PWY89_002591"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2067585.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="PWY89_002592"
     CDS             7746..9092
                     /locus_tag="PWY89_002592"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2067586.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="PWY89_002593"
     CDS             complement(9400..9786)
                     /locus_tag="PWY89_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2067587.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3129 a         1782 c         2009 g         2893 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta tta
//
LOCUS       DANKBF010000093         9595 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000093 DANKBF010000000
VERSION     DANKBF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..643)
                     /locus_tag="PWY89_002594"
     CDS             complement(<1..643)
                     /locus_tag="PWY89_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2067588.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTK"
     gene            complement(645..1409)
                     /locus_tag="PWY89_002595"
     CDS             complement(645..1409)
                     /locus_tag="PWY89_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL2067589.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1417..2094)
                     /locus_tag="PWY89_002596"
     CDS             complement(1417..2094)
                     /locus_tag="PWY89_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL2067590.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2131..3516)
                     /locus_tag="PWY89_002597"
     CDS             complement(2131..3516)
                     /locus_tag="PWY89_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2067591.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3726..3810)
                     /gene="ffs"
                     /locus_tag="PWY89_002598"
     ncRNA           complement(3726..3810)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWY89_002598"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3833..4348)
                     /gene="tadA"
                     /locus_tag="PWY89_002599"
     CDS             complement(3833..4348)
                     /gene="tadA"
                     /locus_tag="PWY89_002599"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2067592.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4455..5312
                     /locus_tag="PWY89_002600"
     CDS             4455..5312
                     /locus_tag="PWY89_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2067593.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5393..5728)
                     /locus_tag="PWY89_002601"
     CDS             complement(5393..5728)
                     /locus_tag="PWY89_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067594.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5795..7165)
                     /locus_tag="PWY89_002602"
     CDS             complement(5795..7165)
                     /locus_tag="PWY89_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL2067595.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7243..7779)
                     /locus_tag="PWY89_002603"
     CDS             complement(7243..7779)
                     /locus_tag="PWY89_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2067596.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8088..9569
                     /locus_tag="PWY89_002604"
     CDS             8088..9569
                     /locus_tag="PWY89_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2067597.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3216 t
ORIGIN      
        1 tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta
       61 ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga
      121 ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa
      181 ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca
      241 tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga
      301 taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa
      361 ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga
      421 ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg
      481 tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata
      541 gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga
      601 acaatccagc atattccatc cactgccatc tcgaattttt catattagaa gctccattca
      661 tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt
      721 gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct
      781 gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg
      841 gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct
      901 gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata
      961 ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta
     1021 actacttttt cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct
     1081 tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca
     1141 tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg
     1201 tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt
     1261 tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat
     1321 tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct
     1381 aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt
     1441 aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga
     1501 ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac
     1561 tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg
     1621 agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt
     1681 gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg
     1741 attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat
     1801 ataatcatat cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc
     1861 ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc
     1921 taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc
     1981 caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt
     2041 agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc
     2101 accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta
     2161 atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc
     2221 aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt
     2281 attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat
     2341 tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata
     2401 gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc
     2461 acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg
     2521 attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc
     2581 cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa
     2641 gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg
     2701 cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc
     2761 ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg
     2821 gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat cattgattac
     2881 gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat
     2941 ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc
     3001 cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc
     3061 ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt
     3121 cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt
     3181 atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt
     3241 caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc
     3301 aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca
     3361 acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt
     3421 ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg
     3481 cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct
     3541 ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc
     3601 tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag
     3661 gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg
     3721 tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac
     3781 acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt
     3841 cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt
     3901 cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt
     3961 taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat
     4021 acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac
     4081 aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac
     4141 aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa
     4201 ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt
     4261 catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt
     4321 ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt
     4381 tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac
     4441 aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa
     4501 aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct
     4561 ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg
     4621 taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg
     4681 ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa
     4741 aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt
     4801 atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg
     4861 aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg
     4921 ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa
     4981 tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg
     5041 tggctttaca ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca
     5101 tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag
     5161 gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa
     5221 tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt
     5281 gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa
     5341 ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca
     5401 ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat
     5461 caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag
     5521 aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa
     5581 atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca
     5641 ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc
     5701 cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca
     5761 tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga
     5821 aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata
     5881 ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt
     5941 gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg
     6001 ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac
     6061 gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt
     6121 tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga
     6181 gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat
     6241 gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt
     6301 tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc
     6361 ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa
     6421 gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt
     6481 gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac
     6541 agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc
     6601 cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga
     6661 caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat
     6721 tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc
     6781 ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa
     6841 ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc
     6901 tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct
     6961 cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc
     7021 ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag
     7081 aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg
     7141 gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt
     7201 acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt
     7261 taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat
     7321 ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac
     7381 caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg
     7441 ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc
     7501 atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca
     7561 ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc
     7621 gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa
     7681 ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag
     7741 caaatattct ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca
     7801 tctacccata aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca
     7861 tatttagtta tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca
     7921 aaatacccta ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta
     7981 tataaaaaag aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac
     8041 agtttttgtc taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag
     8101 caagaaaaaa agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac
     8161 aagaattatc caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa
     8221 taaatgtatc actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag
     8281 gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa
     8341 aattatgcta cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc
     8401 cacaaactgc tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc
     8461 tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat
     8521 ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact
     8581 tttttctttc agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg
     8641 cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata
     8701 tctacttgct gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa
     8761 cttcttctga aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat
     8821 actttacttc tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact
     8881 ttaattattt tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga
     8941 ttggtttttt attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga
     9001 cttacttaac tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt
     9061 attttcttgt gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt
     9121 ctacactttg ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga
     9181 aattaagaga aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc
     9241 aataccttag tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca
     9301 tccatttgca gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc
     9361 agttatttcg caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca
     9421 ttacagatca gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc
     9481 acaatacagt gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag
     9541 gttatgagaa caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttatt
//
LOCUS       DANKBF010000094         9569 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000094 DANKBF010000000
VERSION     DANKBF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..825
                     /locus_tag="PWY89_002605"
     CDS             <1..825
                     /locus_tag="PWY89_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2067598.1"
                     /translation="KDDVLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFK
                     FANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYS
                     LITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSS
                     NAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSH
                     MLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(907..2679)
                     /locus_tag="PWY89_002606"
     CDS             complement(907..2679)
                     /locus_tag="PWY89_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2067599.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(2682..4394)
                     /locus_tag="PWY89_002607"
     CDS             complement(2682..4394)
                     /locus_tag="PWY89_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2067600.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4509..5177
                     /locus_tag="PWY89_002608"
     CDS             4509..5177
                     /locus_tag="PWY89_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL2067601.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5244..6032)
                     /locus_tag="PWY89_002609"
     CDS             complement(5244..6032)
                     /locus_tag="PWY89_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2067602.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6115..7587)
                     /locus_tag="PWY89_002610"
     CDS             complement(6115..7587)
                     /locus_tag="PWY89_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2067603.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(7717..8910)
                     /locus_tag="PWY89_002611"
     CDS             complement(7717..8910)
                     /locus_tag="PWY89_002611"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2067604.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(8908..8987)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(9176..9247)
                     /locus_tag="PWY89_002612"
     tRNA            complement(9176..9247)
                     /locus_tag="PWY89_002612"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9212..9214),aa:Glu,seq:ttc)
     gene            complement(9259..9332)
                     /locus_tag="PWY89_002613"
     tRNA            complement(9259..9332)
                     /locus_tag="PWY89_002613"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9296..9298),aa:Ile,seq:gat)
     gene            complement(9345..9415)
                     /locus_tag="PWY89_002614"
     tRNA            complement(9345..9415)
                     /locus_tag="PWY89_002614"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9381..9383),aa:Gly,seq:tcc)
     gene            complement(9420..9492)
                     /locus_tag="PWY89_002615"
     tRNA            complement(9420..9492)
                     /locus_tag="PWY89_002615"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9457..9459),aa:Phe,seq:gaa)
     gene            complement(9496..9568)
                     /locus_tag="PWY89_002616"
     tRNA            complement(9496..9568)
                     /locus_tag="PWY89_002616"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9533..9535),aa:Asp,seq:gtc)
BASE COUNT         3035 a         2056 c         1596 g         2882 t
ORIGIN      
        1 aaagatgatg tacttgtaaa aaacgttaat agtaatctta tacaagatat gctagattac
       61 tactattttg aaaaaaattt atcatataat tatgtaatac atttaaaaac ttttacagga
      121 atgattttta aattcgccaa ccgtcgatat ggattaaaat ataatcccgt aaatggggtt
      181 tctttagcaa gaaaacctaa gacaaacgaa gaaataaaaa aagaaaaaga tggttattta
      241 tcaaaagaag aagttcaaat gattgctagt aaacaaaaaa gttcacatca acagtcacgt
      301 tattctttaa ttactaaatt tttgttttta actggactac gttatggtga attgatatct
      361 ttgactgaat ctgattatga tggtaataaa attacggttt ctggtactta tgattacgag
      421 ttaaaaatta aaacaacaac taaaaataca ggttcttata gaaccattga attatcaagt
      481 aatgctaaag aaatcataga tcaattaatt gaagaaaaca aattaattaa gaataataaa
      541 gataaataca ttttcatctc taaaaatggt aacccgatat ctattcaagc atataatcaa
      601 tctttaagag cagtatccaa agaattaaac ctagataaaa aagtttcttc tcacatgtta
      661 aggcattccc acatttctct acttacagaa ttaggaatcc cattaaaagc cattatggat
      721 cgtgtaggac atgaagattc aaaaacaaca ttaaagattt atactcatac aactaaaaat
      781 atgcaaaatc aactagtaga aaaactagga aaaatagaaa tttaaattgc cccttttctg
      841 ccccttataa aaacaaacaa cataaaatat ataaaaagaa ccccgttata acgaggttct
      901 tttaatttaa ctttcaaata caaattgatt atgataaagt tccgcataaa atccattttc
      961 tgctaataac tcgtgatgac tgccttcttc aatgacttcc ccatcacgca agacaacgat
     1021 tctatctgca ttcaagattg ttttcagccg gtgagcaatc acaaagctgg ttcttccttt
     1081 gattgcttca tccattgctt tttggatcct tgcttctgtt accgtgtcta cattacttgt
     1141 tgcttcatcc aaaatcaata aagatggatt tgtgatgatc gttcgagcaa tactgatcaa
     1201 ctgcttttga cctgtactga ataagttatt ttcttcggat atctctgtat cataaccttt
     1261 ttctaacgtc atgatgaagt catgaatatt cgcttgtttt gctgcattga tgacttcttc
     1321 atctgaagcg tccggtttgc cgaatgtgat attgtctttg atcgttccgg agaacaatac
     1381 agattcttgc aaaacgattc ctacatgtct acgtaatgca tcaagatcat aatctcggat
     1441 atctattcca tcgatgacta ctgaaccatc attgacatcg taaaaacggt ttagcagatt
     1501 catgatcgtc gttttccccg atcctgttgg accaacaaga gcaaccattt ctcctttgtc
     1561 cacattgatc gagacatctt tcaagactag tttttctgga gaataaccaa aatctacgtg
     1621 ctgcaacgca agaccttttt caacaccatc aaatgcttta cagttttctg gtttgacttc
     1681 atctttttca tcaaatattt cattcaatcg tcttgcccct gtaatcgcta attggatcat
     1741 gctgtatcca gatgaaatct gcatcagtgg ttggtaatat tgttgtgaat actggacgaa
     1801 tgtcacaact aagcctaatg ccaccgaacg ttccaaatca ccatttaatg ctaaccagcc
     1861 gccgaaaaag ataactatcg ctgtattgac cagtgacatt ccttgcatca tcggaaatag
     1921 tagaccagaa taagcttgtc ctttgaaggt tgcttcgcga acactttgat tatgctctaa
     1981 gaatccttca atcgtttctt cttgtaagcc atttgtgatg atgacacgtt gtccgctgat
     2041 tttttcatcc atataaccat ttagtttccc cacttcatct tgctgaagat ccacatattt
     2101 ccgtgctttg cggataatca agacagctat caaaacggct actggagtgg aagcaattgt
     2161 agcccaagct aactctacat tttggcggaa catcatgatc aagatcccga caagtaaaac
     2221 agcattcgtg atgacttgaa gcaaagcttg gttcaaacta ttttgaatat tatctaagtc
     2281 actggtaaaa cgacttaaaa tttcaccgtc ttgatgagaa tcaaagaaac ggatcgtcaa
     2341 tttttccaat ttgttgaata gaccgatacg cattcggttc gttgacttcc ctactacttg
     2401 tgtgaacaaa atactataga taaagttggc tgcactagct agtacataaa acagcaagag
     2461 tttccatatt acagaaacaa atttgctttt atcgtctacg ccttgcatca agcctcctac
     2521 gtaattagct aattcttgaa aagcttcccc gatgtattga ggtgccttta cttgtagata
     2581 ggtagcaatg atcactgtga taaagataaa gaaaaaggag agtttgtacc gcttcaaata
     2641 gtgatagaaa aatttacttg cttttattag atcagtcata cttactcctc cgttcctttt
     2701 tgagtttcaa agatttcctg atagacacgg tttccagcta ccaattcctc atgggttcct
     2761 tcgccgacta atctgccttc atccaatact aagatgcggt ctgctttgac aacagaagca
     2821 attttttgtg cgatgacaat cgtcgttgtt cccttcaaat ctcgatcgag tgcttctcgg
     2881 accagctttt cagagcgagc atccagtgca ctcgtactgt catctaggat caagatcttt
     2941 ggattaccga taacgccacg agtaatggat aaacgttgct tttgaccacc agaaaagtta
     3001 ctgctacgtt cttctattgg tgcttcgtaa cgatcagata acttttcgat gaattctttt
     3061 gcttgagcaa tagaagaagc atgttccatc tctttttctg ttgcattctt tttcccttgg
     3121 cgcaagtttt gagcaatcgt tcctgagaat aaaattgctt tttgcaaaac aaaggaaacc
     3181 gtttgtctta aactcttttc atttacttga cgaagatcta caccacctac ttcaactttg
     3241 ccttctgtcg gatcaaatag acgagggatc aattgtgcta gtgttgactt cccggcacca
     3301 gtcgccccga cgataccaac catttctcca ggtttgatag aaaagctgat atctttaagc
     3361 gtatctgtat cctcaccagg ataacgaaaa gaaacatggt caaaagtgac agaacccgtc
     3421 aactcttgtt ctggaacgtc taaataagta agatctggtt cagcatctaa aatttccgca
     3481 atccgcttca gcgatacggc tgctcttgaa gtcatcatca tcatcattcc gccaataatg
     3541 attgccatca taatctgcat caagtagttc atgaatgaag caattccacc aattaacgta
     3601 ggatcatctt taacaagatc gctcacaaaa aagatcgccc caaccacagc caaattggca
     3661 gccagcatga aggacgggat catcacagaa aataatgtac cgactatgat gttgtgtttt
     3721 gttaagtcct cacttacttt gctgaatgac ttcaattggt tttcttcttg tacaaaagat
     3781 ttcactacac gaatccctag taagttttct tttgcaatgc tattcacacg atcaatcagt
     3841 ttttgaatga tcatgaagtg cttgcccatc cttgtaaatg aaagtgccgt aatcagaaat
     3901 accgtaacga taagcgctac gatgatccac cataattgcg gcatcgtgat catagctaaa
     3961 atgaaggcac caataaataa gaatggaatt cgaaaaagtg attgcaaaga aatcatcact
     4021 aaattttgga tttgcgtgat atcatttgtt agacgaacaa caagatttcc agcagagaac
     4081 tgttcgatat ttccaaatga aaatgtctgg atctttcgaa atgcttcttc tcggatatct
     4141 gcacttacgc cttgagctac ttttgcagag aaaatcgtgt tcgttacacc tgcggctaat
     4201 cctagcaaag ccagactgat caggtaaata ccaattgttt tcatttcatc attatcttct
     4261 gtaataatag cttccaatac ttgctgtagt aacttaggtt gccataatgc tgctgcaacc
     4321 atgactgcta cggacagcaa ggaaataaaa acagctactt tatatttttt cgcatgtcgt
     4381 aagattaatt tcattcgttg attacacccc tttgtttaaa ttgacaggac taatttaaca
     4441 ctgtaaaatt agaccgacta acaatttacc atgtttaaaa agataatgca atacacgagg
     4501 ttgataaaat ggaaataaag ctttctcaag caatcatcat tcaggccgcc tttgatatcc
     4561 tggctgaaac acaagctctt tcgcagcttt ccatgcgaaa catcgcaaac agaattcatg
     4621 ttcaagcacc tgctttatat tggtatttca aaaacaaaca acagctcttg caaataatgg
     4681 ctgaaacaat ggaagaagaa ttagcgcttc cagatccaac tttgccttgg aatgaacagt
     4741 tgctgcagta tatggaaaat tactatgacc tttatacacg tttcccttgt ggggcagaat
     4801 tagaaatcaa tactgtgccc gcttttccga gtcgattaga acatttggag caaatgatcc
     4861 aattacttgt tcgtgaagga ttttcagtca cccaaagtca tcaggccatt gcctctctcc
     4921 acaatttatt gattggacag ttaatggatc agcagcatga acagcagctt cgacaaaaaa
     4981 ttatggacgg aaatgaattt ttgaaagaaa cagttctatt tatgcgtaat tatgtagttg
     5041 aaaatcaatt agagggattt aaagaaagta tgcagtccca tgcagaattg aatgaaaaag
     5101 cactctttct aacgagtgca caaacctata ttgaaggctt atgccaacaa aataaaagaa
     5161 gagaaaacga gcagtaaaaa agctcctata gagatcacta tgtttgtgat ctctatagga
     5221 gctatctttt taagactctt tttttatttt tcttccatta gccacgccaa tgcacgttga
     5281 atccattggt cccagaagat ccagttatga tcgccaggtc cttgttcaaa ggtgaccgcc
     5341 aagccttgtt cctgcatagc agataccatt tcctgatttg cctgataaag aaaatcagat
     5401 tccccacaag ccatataaaa tcttggaact ggttttccgc tttttactag ttctttcaat
     5461 aagtattgcg gatcattttt tgaaccttcg atttgatcca atggtccaaa gatcccttcc
     5521 caaaaagatg ctttacgaac catcaaaaga tcatccattc gatttcctac agaaacagcg
     5581 cctgaaagag agattactgc tgcgaaattt tctgattttg caaggcctag ttttaacgcg
     5641 ccatatccgc ccatcgataa tccagcggca tatgtttttt ctctttttct tgttaattgc
     5701 gggaataatt catgacaaat ctcaggtagc tcttctgcaa tgaatgtcca atacttcatg
     5761 tcataaactg tatctgtgta ccagccaaga tcagtagatg gcatgatgac agctaatccg
     5821 tagtttgcta cataacgttc aatcgacgta cgtctttccc atacactatg attgccaccc
     5881 attccatgaa gtaaatataa aacaggtata tccgttgtct cttgctctga actggtgcca
     5941 atcacctttt ttgttgtttg cggtagtatc acatttagat tgacttccat ttctaacaca
     6001 ttcgaataaa tatttgcttg aataaatgcc atattttcgc cttccttagt aagaagttat
     6061 ttttgaataa ctttaaattt gaatgattcg gctacgtaca tatcttaacc gaaactactg
     6121 ttttatttta ccactttttt catgaacgaa aagcgttaag accaaagcaa tgatcacgag
     6181 taacaagcca actagataaa cggaatgtaa cccatcataa agaatttttc ttaatggtaa
     6241 caaaatatcc gcatcaatat atttagccgt ttgtggattc accagtttat tcatgatatc
     6301 tgcatcattt gttaaatttg ctgctgcaag ttctcgttta gttaccccat ttatcaataa
     6361 accaaaaata gaaaccatga ctgtttgacc aattgttcga actaacgtat taaaagaagt
     6421 tgctacacca agctggtcac gagatacact attttgtgca ctgacagtcg aggtagtcat
     6481 ggtcacacct aagcctatac cgatgaccgt tgatatagca aaaaaccata aatagttact
     6541 tctgaaaggc ataagataca accaagcgcc cccaatagct gcgattagta gtccaattct
     6601 caagacccaa tgagttttca tttgagtcat ccacttgctt gcaaggaaag aaccgaccat
     6661 ccagactagt gataatggtg ctaataccag cccaccaatt gctgcattca gccctaatac
     6721 accttgcatc cacataggga tataaacatc gattcccatc aaaaaaccac tagccaatgc
     6781 tgcagccaag ttgaccacaa caaatagtgg atttttgaat aaatgcatca tgatgacagg
     6841 atctttagct cttttttcgg caaaaacaaa aatacctaac attacgatac tacctagcag
     6901 caaagcagaa acaagcggac tcattccttc gtctcctaat aattggaacc ctaacagcaa
     6961 tgagacaagc atggccatca aactaaaact accaaaaata tccattggtt tagactcatg
     7021 tatgattttt ggttcattta aatatagcca aatcaaaata attaatagaa ttccgatcgg
     7081 cacattgata aaaaagatcc agtgccagct gatcgtttca acgatgaatc ctcccgcaag
     7141 cggtccaaaa acactggcaa tcccccaggc tgaactattc aaacctaaaa cttttgctcg
     7201 cttttcaata gaataaagat cgccgatgat cgtcaaagct actggcatca ttgccccagc
     7261 acccatccct tgaatcgcac gagcagcaat caacccaatc atgctttgag ataaaccgca
     7321 aaataacgaa ccgataataa acaataatgt cccaaaaata aagattggtt tccgtcccac
     7381 tttatccgca agttttccgt aaatcggagt aagcatagcg tttgtcagca aataaatcga
     7441 aaatacccag ttcatgattt ccattccatg taatgaaccg acaatcgttg gcatagcggt
     7501 tgttacgatt gtaccttcaa tagccgtcat gaacgttgca atgaaaatac tcactgtgac
     7561 caaagtaaca tttgtttctt ttttcatctt attcctccct ttcattatga caggatctaa
     7621 aaatagataa ataattacta tctttttcaa gaggttaact gccataaaaa aagctggaac
     7681 aaccaccact tggcgatcgt tccagctgaa tataacttat tttgctaaac ttgctttcaa
     7741 cgcatctact ttgtccgtat gttcccaagg caaatctaca tctgtacgac caaaatgacc
     7801 gtaagcagct gtttgtttgt aaatcggacg acgcaaatca agcatctcga tgattcctgc
     7861 tggccgaaga tcgaagtttt ctctcactgc agcaatcagt tcttcttccg gaaccgttcc
     7921 agtaccgaaa gtattgattg agatcgaaac aggttgagca acaccaatcg cataagctag
     7981 ctgtacttct gctttgcgag caagccctgc tgcaacaatg tttttagcaa tataacgtgc
     8041 agcatagctg gcagaacggt caacttttgt cgcatcttta ccagaaaaag caccgccacc
     8101 atgacgagca taaccgccat acgtatcaac aatgattttt cttcctgtta atccggcatc
     8161 cccttgagga ccaccaatga caaatcggcc agtcggattg atataatatt tcgtttgatc
     8221 atctaataat tcagctggaa taacttcctt cactactttt tcgatgacat catgacgaat
     8281 tgtttcatta tctactgcat catcatgctg tgttgaaata acgattgtat ctacgcgttc
     8341 cggttgtcct tgatcatcat attcaaccgt tacttgagat tttgcatctg gacgtaaata
     8401 cgttaattca tttgatttac gcaaatctgc caagcgtcgt accagacgat gactcaaagc
     8461 gattggtaaa ggcatcaatt ctggtgtttc atctacagca aacccaaaca ttaaaccttg
     8521 gtcaccagca ccaatctcat ctaatacgtc ttttttatct tcatcccgga tctcaagtgc
     8581 ttcatcaact ccttgagcaa tatcaggaga ttgttcatca atcgcaacca atacagctgc
     8641 tgtatctcca tcaaatccga attttgcacg tgtataacca atttctttta ttgtttcccg
     8701 tacgactttt tgaatatcta catatgccgt cgtagatatt tctccaaata ctaaaacaag
     8761 accagttgtt acagacgttt cacacgctac tcgtgctgtt ggatcttgtt ttaaaattgc
     8821 atccaagatt gcatcactga tttggtcagc aattttatct ggatgtcctt cagaaacaga
     8881 ttctgatgta aataagtgtc tttctaccat tataatgatt ccccctagtt aaattcggtt
     8941 acaaggcatc tccgtcctgc ggatcctttc gggaacttgc aacgaaccaa gtatagcaga
     9001 taaaacagtt ttctgctcgt attttttcat ttttttgctt aaaaaatata aaaatatttt
     9061 acattagaac tgtccaaaaa agcttagcac aatcatccat aaagataact ctgctaagct
     9121 ttttaaaatc attctttcag ataaaaagaa aaagatactc aaaaagtatc ttccatgacc
     9181 cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca
     9241 acgggccaat aaaataaatg ggcctaaatg gactcgaacc atcgacctca cgcttatcag
     9301 gcgtgcgctc taaccagctg agctataggc ccataaaaac taaaagcggg tgacgagaat
     9361 cgaactcgcg acaacagctt ggaaggctgt ggttttacca ctaaactaca cccgcataat
     9421 ggcgcgggac agaatcgaac tgccgacaca tggagcttca atccattgct ctaccaactg
     9481 agctaccgag ccaaacggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca
     9541 ggcatgttaa cccctacacc acggaacca
//
LOCUS       DANKBF010000095         9527 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000095 DANKBF010000000
VERSION     DANKBF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..818
                     /locus_tag="PWY89_002617"
     CDS             <1..818
                     /locus_tag="PWY89_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2067605.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVD
                     RLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLP
                     QIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
     gene            938..1138
                     /locus_tag="PWY89_002618"
     CDS             938..1138
                     /locus_tag="PWY89_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2067606.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1214..1411
                     /locus_tag="PWY89_002619"
     CDS             1214..1411
                     /locus_tag="PWY89_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067607.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1708..3255)
                     /locus_tag="PWY89_002620"
     CDS             complement(1708..3255)
                     /locus_tag="PWY89_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDL2067608.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3339..4142)
                     /locus_tag="PWY89_002621"
     CDS             complement(3339..4142)
                     /locus_tag="PWY89_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2067609.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="PWY89_002622"
     CDS             complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="PWY89_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2067610.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="PWY89_002623"
     CDS             complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="PWY89_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2067611.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="PWY89_002624"
     CDS             complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="PWY89_002624"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDL2067612.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6527..7456)
                     /locus_tag="PWY89_002625"
     CDS             complement(6527..7456)
                     /locus_tag="PWY89_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2067613.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7651..8334
                     /locus_tag="PWY89_002626"
     CDS             7651..8334
                     /locus_tag="PWY89_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDL2067614.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8495..>9527
                     /locus_tag="PWY89_002627"
     CDS             8495..>9527
                     /locus_tag="PWY89_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2067615.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGD"
BASE COUNT         2918 a         1949 c         1891 g         2769 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga accttcttga
      301 atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct caacgagggt
      361 tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgtcg
      481 atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac agagaagtca
      601 gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta aacaaccaca
      721 tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc aaactacgga
      781 tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa gagagtttct
      841 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
      901 gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg aacaaaaaaa
      961 catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga taggcgagtc
     1021 aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt caattgaagt
     1081 gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag aaaaataaaa
     1141 ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt cttttctttg
     1201 agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca attttgtgaa
     1261 agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg aaaaagattt
     1321 cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc tagacaaaga
     1381 gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt attttttgta
     1441 tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt ttcagaaatg
     1501 aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt cttatttaag
     1561 gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa agaatggctg
     1621 gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa aaactgctca
     1681 cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat gattcacttt
     1741 tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca tgtcgatttg
     1801 gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc cgattgcccg
     1861 atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac ctttaaaacg
     1921 aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact gaatcccttc
     1981 atacatgtca taattgtagt actggggctt aggctgaata aaggctggat tgaatacgat
     2041 ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc tcaaatctgc
     2101 accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga tgacgtcccg
     2161 catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag ttccagaaat
     2221 ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg gatgttcctc
     2281 aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac ctaagggttg
     2341 atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa tttctggatc
     2401 aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa ccacttgccc
     2461 ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat agcctggttg
     2521 aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac cggtgaaaag
     2581 ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc cagttagcgc
     2641 ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac taacgattac
     2701 caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga cgatactctt
     2761 aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt gtgtcgttaa
     2821 tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt ttccaaagta
     2881 tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat aattcttaat
     2941 cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca cttgaacatc
     3001 atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt ctttagcaag
     3061 ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa ttaccggtcc
     3121 atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc ctaaggccag
     3181 atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac ttgtctctaa
     3241 aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa accgaagcgt
     3301 attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat ttgaagtgtt
     3361 tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct tccttgatcg
     3421 agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc gccttcatct
     3481 tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat ttcatcagta
     3541 aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc gattagcttc
     3601 aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag tagatgatga
     3661 tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt cagccattga
     3721 aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc aaaggaaatc
     3781 actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc ggcttcatcg
     3841 caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac aagaatcgtc
     3901 gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc tatttcgcca
     3961 tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg caggagatga
     4021 tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat tcccttatag
     4081 ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg caatatattc
     4141 attcttatct caatctcttt cttataattc cggttatttg atctaaaata aacacggtta
     4201 cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga atcgcgaata
     4261 tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca ctacgaaccg
     4321 ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag gggatgcgaa
     4381 caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca acaagtcgct
     4441 catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc tgatggacgc
     4501 caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa ataattgcaa
     4561 aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt tttgttaccc
     4621 atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc gctaataccg
     4681 tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa ctaatcaaat
     4741 cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct ctcaaaacgt
     4801 ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta gcggccgaca
     4861 aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc atttcacgaa
     4921 gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg cgacagttac
     4981 taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa gtgaggaatt
     5041 tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag cacgaacatt
     5101 cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg gagcgatcga
     5161 ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa tcgggccaaa
     5221 gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag taaagacagc
     5281 gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa gcgcggctaa
     5341 aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg tagaaagaag
     5401 acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta aaattaagcc
     5461 aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga ctaatttagg
     5521 taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt gattggagtt
     5581 ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa aacaaacaat
     5641 taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt ctttcaagct
     5701 catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc tctaagcgtt
     5761 catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt aacgcgagca
     5821 cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg actgttttat
     5881 ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg attggatcta
     5941 atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt gctcgcgcaa
     6001 tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc aatttatcag
     6061 acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc gtaaaaagac
     6121 ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca tttttctgaa
     6181 ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg cggcgaatct
     6241 gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga atatctcctt
     6301 tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca gcaccactca
     6361 accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc ttcaagccaa
     6421 caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc cttctccatt
     6481 tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat tctgctgctt
     6541 ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca tagccctgat
     6601 gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt aagaaggtct
     6661 ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt gcgtcattcg
     6721 gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca ttatctggga
     6781 aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc atttgcttgt
     6841 tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt gtatctgcag
     6901 taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg ctagcagagt
     6961 taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc aatccgctat
     7021 cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt aatggcaagc
     7081 cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca gctccagcat
     7141 cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga ccagaagaca
     7201 tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca cgatctaact
     7261 ttttcgataa atacttagca aatggttttg cttttgcctc catactacca tcattgtttg
     7321 ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg ctacttgtat
     7381 tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac ccttttgcta
     7441 actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg tctaaatcat
     7501 agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact tttacataca
     7561 atttacaaac aattataaat aattattcgt attgagccga aaaacaatct aagctataat
     7621 aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac gaagcaatta
     7681 actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat attggcaatg
     7741 gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa aacctcagat
     7801 tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc tgatacacaa
     7861 aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc ttcattgtgg
     7921 gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat cagcattccg
     7981 ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa tatttgccag
     8041 ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac agaacctgtt
     8101 gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt ctcatttcgg
     8161 ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg aacaatcacg
     8221 aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat tcatgatatc
     8281 gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga ataacagcta
     8341 taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa ttgggaaacg
     8401 gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt taaaagttca
     8461 atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg tttcattgtt
     8521 gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg agaagcaaag
     8581 tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg atgaccgctc
     8641 cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa agctgacagc
     8701 aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta ccaatatcga
     8761 tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc aagcagagga
     8821 actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag atggtgagat
     8881 tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg cgtatttagc
     8941 aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg gtggaatgga
     9001 agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg caggggccac
     9061 agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg attcgctaga
     9121 taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc tttatcaggc
     9181 ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg aaaacgacta
     9241 tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag gggattataa
     9301 aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa atgtcggtgc
     9361 agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg gcaagcattt
     9421 gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat cagaggaagc
     9481 ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtgatt
//
LOCUS       DANKBF010000096         9435 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000096 DANKBF010000000
VERSION     DANKBF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            50..123
                     /locus_tag="PWY89_002628"
     tRNA            50..123
                     /locus_tag="PWY89_002628"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84..86,aa:Ile,seq:cat)
     gene            complement(508..1005)
                     /locus_tag="PWY89_002629"
     CDS             complement(508..1005)
                     /locus_tag="PWY89_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2067616.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1045..1854)
                     /locus_tag="PWY89_002630"
     CDS             complement(1045..1854)
                     /locus_tag="PWY89_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2067617.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2188..3045
                     /locus_tag="PWY89_002631"
     CDS             2188..3045
                     /locus_tag="PWY89_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2067618.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            3038..3940
                     /locus_tag="PWY89_002632"
     CDS             3038..3940
                     /locus_tag="PWY89_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2067619.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3973..4323
                     /locus_tag="PWY89_002633"
     CDS             3973..4323
                     /locus_tag="PWY89_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2067620.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4372..5295
                     /locus_tag="PWY89_002634"
     CDS             4372..5295
                     /locus_tag="PWY89_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2067621.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5373..6266
                     /locus_tag="PWY89_002635"
     CDS             5373..6266
                     /locus_tag="PWY89_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2067622.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6304..6867
                     /gene="amaP"
                     /locus_tag="PWY89_002636"
     CDS             6304..6867
                     /gene="amaP"
                     /locus_tag="PWY89_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2067623.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6881..7069
                     /locus_tag="PWY89_002637"
     CDS             6881..7069
                     /locus_tag="PWY89_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2067624.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7082..7984
                     /locus_tag="PWY89_002638"
     CDS             7082..7984
                     /locus_tag="PWY89_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2067625.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            8016..8258
                     /locus_tag="PWY89_002639"
     CDS             8016..8258
                     /locus_tag="PWY89_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2067626.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8514..8885)
                     /locus_tag="PWY89_002640"
     CDS             complement(8514..8885)
                     /locus_tag="PWY89_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067627.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3312 a         1617 c         1819 g         2687 t
ORIGIN      
        1 tcattcgtgc tatagttgaa aagtaacaaa aataaatttt atttatgatg tacctttagc
       61 tcagttggtt agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacaaggt
      121 acataagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aagtaaaaac
      181 agcttcttat gtttttactt ggctgttctt attactgcag atgcaagctt ttgaataccg
      241 tttaaggaag aggttgtgaa atcagcgttt tgacctgagt attagcttaa caaacggaaa
      301 aatagttcct catattttgc cgtttgttaa gctaataccg agggtcagct gattgaacac
      361 catttaagaa agaggtcgtg agcctgccgt ttcactctgt gtcataaaaa agccaatcta
      421 ctttgataga aaaagcacgt aggactaaaa cagttctgcg tgcttttttt attgtgtgtt
      481 aaatatcgta aatgaagata gatattttta tgattcgacg atagagacgt tgaaatcttt
      541 caacagctct aaagcggatt ctccgtgttt tctactggag actaacattg gatcaacaaa
      601 aaagtctgct tcaggaaaag cactctgcag gcctatcaga ttagagagta cacacaagtt
      661 tgtttcaatc ccgactactt cgatctcttt ttgttcttct ttgctttcaa ataaagtttc
      721 ctggatttca gctaaagttt caggtggaat cgtaaagtaa tactttttaa tcactcgttc
      781 attttcttgt ggagaaagaa gagggatcaa ttgcaaatct tctcgttctt cttctttgtc
      841 cttcagttca actggtatat cccgtgtgaa gagaatatat tcattatttt ggcgtgcttt
      901 agctaatctt tgagcaatac gttctgccaa ttgatctgac tctggaatat aaaattcgct
      961 gtttggatca agaatatgat tttgcatatc aataataact aacattttct ttcctcctac
     1021 attcatatcg tcattttatt ctttttaata gaagtttttt aaaatataag caacaccagc
     1081 ttcttcattc gtcagtgtta cttggtttgc aatttctttt acttgtcgct gtgcattccc
     1141 catcgcaata ctcaacccgc tgtaattcaa catagcaatg tcgttttctt gatcgccaaa
     1201 agccatgatg tcactcgatt cgattccaag gtaatccgcc agaaaaacaa gtgaatgagc
     1261 tttagtgata cctgtaggcg tgatttctaa gaaatgcgga tcagagaata ctgattggat
     1321 accatctgct tgcagatctc tttgtagctt ctgtagtttt tcttcactct tatctgttaa
     1381 tgctaattta agatagctaa gagaaggatc ttccaacata tttaaaattt gatcaaaaga
     1441 atggttctgt aaattgaaat gttcataata agggtcatct ttgactagat ttgattcatc
     1501 gaatgtcagc tgatcatctt gggtttctcg gctagcaatg atggatattt tcgcttcttg
     1561 agcatagaag aaggcttttc tcagtgtatc gctatctatt tttgaacgtc tgagaacttt
     1621 cgccattttt ccatccttca tttcagcaat cagtgaacca ttggaagaaa tcaaatagcc
     1681 accgattcct aattcggaaa aaatatcttt agcagaaatg ctgttccttc cggtagcgac
     1741 ggttacgatt ccgccatttt catcaaattt ccgaagtgct tggatatttt cttctggtat
     1801 ctttccattt cgatcaagca aggtaccgtc taaatcaatc gctgctagtt tcattttatc
     1861 ttcctacttt ctttttacat atctattttc ctgttacctt aagattaaat gagaatttaa
     1921 aaaaatgaaa ctaacttgct ttgtcattat gaattttttc aaaaaacatt cttaagaaaa
     1981 aggtggctat atatagtagt ttttggtcaa tacctttgaa taaaaagcca tgaaaaacga
     2041 atagttagcg cttaccgatg cgttttgtga taaaaaaatt ttaaaatatt caatgaaaac
     2101 aaagcatata ttttgcttta aaaccaaatg aggaatattc tagatgtaga acaggttaga
     2161 aaaagattag aaaaaggagt gattttgatg gagaagagat acggcgtagt tcctgtggta
     2221 actgcttcag acgaaaatta tgctccttac ttgagcgtga tgatcgcaac tgcattagaa
     2281 aattgcaata aaacaagacg cataaaattt tatgtgattg acgatggtct atcagaatat
     2341 agtaaagaag gattggaaga aacggtaaat aaatattcaa gtaatgcaag tatccaattc
     2401 ctgacagtgg aaaaagatat ctacgaagac ttcctagtca gtgaccatat cacaactacc
     2461 gcttacttaa gaatttcttt accaaactta ttagctaaag aggattataa gaaagtcctt
     2521 tacttagatt cagatgtgtt agtactagac gatatcgtaa aactatatga tgagccgtta
     2581 aatggaaaaa caatcggcgc tattatcgat ccaggccaag tgaaagcttt ggaaagatta
     2641 ggaatcgatt ccgatgactt gtacttcaat tcaggagtga tggtgattga cattgatcaa
     2701 tggaataaaa aagaaattac agaaaaaact atccattacc tcagtgaaaa tggcgatcga
     2761 atcatctatc acgaccaaga tgctttgaat gctgtacttt atgaagattg ggaacaactt
     2821 catccaaaat ggaacatgca gacttcgctg atttttgaac gtcatccagc accaaatgaa
     2881 aaatatgaac gtcagtataa agaaggaaat gaaaaacctt cgatcgttca ctttacagga
     2941 catgataaac cttggaatac gttgaaagat catccgtata ccaatttata cttaaaaaaa
     3001 ttagcacaca gtactctaac gaaagtaggc gagatcaatg aataaaaaag aaatagcagt
     3061 agtcgcaagt tgtaacacaa aattcgtacc acatttagct gcattatttg tctcagtatt
     3121 agataattgc aacccgtcta aatttgttcg gttctatgtg attgacgatg atatcgattt
     3181 tgaaagtaaa cagcttttac gcttctcagt gaaaaacgca cgaatgaact cagatgtgga
     3241 attcttgaaa atcaataaag aattctttac aaatgtggta atcagcgatc gtattccaga
     3301 aacagcttat tatcgtattg cgattccgga attgttccgc ggcacagaag ttgaacgtat
     3361 cttatatatg gattgcgaca tgatcgcttt acaagatatc tctaaattat ggcgtcttga
     3421 cttcggtgat tcaatcgtag cagctgttga agacgctggg ttccatcaac gactggaaaa
     3481 aatgaaaatt tcagctaaat caatgcgtta tttcaactca ggtttgatgc tgatcaatgt
     3541 gaaaaaatgg ttagatgaaa atattacgca aaaagtatta gatttcatcg aacataatcc
     3601 agaaaaatta cgattccatg accaagatgc attaaatgct atcttacatg accgttggtt
     3661 accacttcat ccgcgttgga atgcacaagg ctatatcatg gcaaaagcaa aaaaacatcc
     3721 aacagcagca ggtgaacgtg aatacgaaga aactcgcaac aatccttaca ttatccattt
     3781 ttcaggacat gtgaaaccgt ggagcaagga ttttgaaggg ccaacgaaga aatattacga
     3841 aaaatatgca gggatgacgg ctttccgttg tgtagctaaa ttccctaaat atccaaaata
     3901 tgcaaaagtt caaccaaaaa cagatgtagt tgttaaataa tagaagacaa caactgaaga
     3961 gggggagtag gaatggaaat tacaatgtct aaaaatgcag tagagacgtt aattgaaaag
     4021 gttggggaaa atacaaaaat cgcactcgcg cttatcaatg attccgatcc atttctaagg
     4081 gacaaaggtg cttttgcaaa aggcagcttt tttcaaatca ttccttttgt ttcggaattt
     4141 ggagaatatg cgactaaaat cgagcatcca ttattggaca tctatacatc taaattagag
     4201 caaaattatt tcggtaaacg attgaacatg gacttcaaca aacagcttga cagcttttca
     4261 ttagaaaatg aaatcgcagt actcgattat aatatcaaat taaagaactg cttcttcagc
     4321 taatagtttc gagaaagtcc aaaaaggagg tatacattga gatactactt tgtggatgaa
     4381 gaaaagaatg taatagaaac agatgaaaaa aaatataatt ggctcgtact tgatagtgca
     4441 aagcccgaag agattgagca agcaatcgaa gaattcgagc ttcctgatga tattttcgta
     4501 ggaaccgaat atccggaaga agtttctcgg atggagcgcc tttatgagac gaaactagag
     4561 catccatttt ctttggtagt gattaatcta gatcagtcag ataatcgtat cgaaaaaaaa
     4621 ttaacgccaa tatcttttgt actttccgat aatctcttga ttacttgtat cgatagaaaa
     4681 accgaatttg tggatcggct gctgacaaag catggaaatc atttaaactc gtttgaaaag
     4741 atcatcacgt atacattatt agatatttac acccattacg tgaaagaact aagagagatg
     4801 aaaaagcgaa ttgacgcttt ggataaagca gctcgtaaaa caacggaaaa tgaagaactg
     4861 tataaacagg cagatcttga aagagacatc gtatatatcg atcatacctt aagagatcaa
     4921 cgggaaacaa tggatcaatt atggaattct gaaactttca tcaaacgttt agacgatgaa
     4981 cagctactct atgatgtcca tttgagacag cgacaaacag aaaaaatgat cgagatctat
     5041 cgagatttat tagagagtat tggtgatcta ttcaacggga tgatggacaa taatctaaat
     5101 catatcatga aatatctaga ttcagctgca ttaatcattt ccgttccggc gttgttcgca
     5161 gggatttggg gaatgaatac aggaggatta ccgggtaaaa gttctgctct aggctttata
     5221 ctcgtagtct ttggctcttt gatagcagca gtagttttag gttaccattt ataccggaaa
     5281 gattatacaa attaaagaat ctatttaaaa caggtatcaa acaaagaaat ttgttttggc
     5341 aatacaattt aaaaactagg aggaatacag caatgacaga accaaaatta atcgacccgc
     5401 gaaacttgta ccatacggag aaatttccta aacaagatca ggatacacca gctttacagc
     5461 agaatatgat tcctgttcct gattgtggag aagaatccta tgaaggcaat aatcaactag
     5521 aaaaccgccg tgtactaatc actggtggag attcagggat cggtcgtgca gcagcgattg
     5581 catttgctag agaaggggca gatatcgctc ttcaattctt cccaggagaa gaaaaagatg
     5641 cagaggaagt agcaaaatac gtgaaagatg caggacgtaa gattgtctta ctaccttttg
     5701 atcttcgtga cgaacaagca cctaaagaaa tcatagataa agccgtgagt gaacttggcg
     5761 ggctagatac gatggtacta aacgcaggac agcaaatttc ttgtcagtct attgctgagt
     5821 taccacttga gcaagtcaaa gacacattta tgataaacat catcgctatg tttgctttag
     5881 tgaaagaagc tgttccgcat atcccagcag gaggagcaat cgtaacaaca acttctgttc
     5941 aagcattcaa tccaagcgaa catttattag attacgcagc aacaaaagct tcaatcgcta
     6001 actttacgat cggtttagca aaacagcttg cacctaaagg aatccgtgta aatggtgtag
     6061 cacctggtcc aatctggaca ccattacaac tagacgaagg acaaccttct gattcgatcc
     6121 ctgaatttgg tcagcaaagt ttactagaac gtgcaggtca gccaggagaa ttagcaccag
     6181 tttatgtttt cttagcatcg aataaagcaa gttatgttac agcccaaatt tacggagtaa
     6241 ctggtggaga agcgatcaac ttataagaag ttgatcgttt cacccctatg aggaggtgcg
     6301 aaaatgaata aaggtgtaaa aacgatcgga gtgatcggaa cattactttt gctatctgtg
     6361 cttttgtttg cgatactatt aagtgcaacc tatccattgc cttttcagct agagaagttt
     6421 cgattctttt ccatcactaa ttcatacgtg caacaatatg tattttggct tgccgttgca
     6481 tttgctattt tgacaattat cacgttactt gttttcctgt tttatccaaa aacaatcgga
     6541 aactttctac tgaaacatgg tgatggggag ttgacgttgg ataaaaaagc aattgaagga
     6601 atggttcgtt ctcacttgca tgaagatgaa ttcatccatt caccaaaagt aaatgtcaaa
     6661 gcaacgaaaa acaagatcgc cgtcaatgta aaaggggagt tgaaacgaac ttcagcactc
     6721 attggtaaaa caggaacatt gatgaaagaa atcgaaaatg aagtacaaca catattggga
     6781 acaaaagagc cagtgaaagt atcggtgaaa tacacaggct ttgaagaacc agctaaacga
     6841 agcaatgacc gcaaacgagt agaatagggg gggaccaaaa atgaaagaac tattttcgac
     6901 atacaagctc cctatcatct ttggggtact tggattgata ttagccgttt tatggatcac
     6961 cgtgggcttt ttcaagacct tattgatctt gatcatggca gcgatcggcg tcgctatcgg
     7021 tttttatctg aaacaaacga aaatcttaga tcactatttt aaatcttagg aggaaacatc
     7081 catgaataac aataacaaaa ataataacaa taaaatgaac aaccaaaaac cagtgtctgc
     7141 acatccagac ccaacgacac caaaccaatc aaatggtgtg aacgatgaaa agaacaaaac
     7201 agcaccagta tctgcacatc cagatccagt agtccctaac gttcaacaag gagtagaaga
     7261 gaagaaacat gatgcagaaa agcatgcacc tcatcatcca ggaactccag aatctaaagt
     7321 tccagaacaa catgatgcaa tcgatccttc taaacctgtg gaagagcaaa aacatccttc
     7381 tgaccatgcg aaagcacaag aaaaacttag ttctgaccac acaaacggac aaggaaaact
     7441 tggttctgat catacaaata accaaggaaa gcatgattct gaacaggaag attcaatgcg
     7501 cggtgagctg aattatgaag ataaagttgt gcaaaaaatc atcggaatcg caattgaaca
     7561 agtagacggt ttattatctg caaacggtgg tttcttctct aatgtggcag gaaaacttgt
     7621 gaatacagat aatgtaacgg ctgggatcga aacagaagtc ggtaaaaaac aagtagcagt
     7681 tgatatggat gtcattgtgg aatatggcaa agatatcgaa aaaatctttg aagaaatgca
     7741 agaagtaatc ggcaaagaag taaaaaatat gacacattta gaagtgatcg aagtcaacgc
     7801 taacgtagtg gacatcaaaa caaaagaaga atttgaaaaa gatcaagaaa cggtgcaaga
     7861 caaagtaact gacgcagcga aaaaaaccgg tgaatttgct tcgaatcaaa cagataaagt
     7921 aaaatcagca gctggaagcg gcgcacaaaa agtaaaagaa aatacagaac cacgggttca
     7981 gtaataagga agtaaaacat taggaggaac gaaacatggg atttatttgg tcacttatcg
     8041 tcggaggaat tatcggagca attgcaggag caatcacgaa taaaggttct tcgatgggga
     8101 ttattgcaaa tgtcgtagca ggtttagttg gatcagcact tggtcaagca ttattaggtt
     8161 cttggggacc aagtattgca ggaatggcaa tcgtgccatc attgattggt gccatcattt
     8221 tagtactaat cgtatcattc tttgctcgaa aactgtaaaa agacttttaa aaagaggtcg
     8281 tgaaaaagct gttcttcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt
     8341 tttcatttgg ttgggcatat tatcgaagat gcaagctttt gaacactgtt tatacggaat
     8401 aagaggctgt gacaagactt ttgtcacagc ctcttattta tgcgggcaaa ttcccaatta
     8461 gatttttaaa agtttttcct taaatgtgtc tttattgata cagagaagct tctttaataa
     8521 aacaatgcct ccctaaattt tcttgagata ggaaaggtga acatacaaaa taatacgacc
     8581 actttgaaaa cccagatgat ccacaaatgg atcgtatgat actgatgaag taaaaagcaa
     8641 tggatcagaa actcaaagag attgaaaaag acaaggaaga aataatatct cttttttact
     8701 cttgtctgtt ttagccaata atagctcgta aagtaatcta gacaaacaaa tagaagtaaa
     8761 caaagtttcg taagagcagc aatcgtatca tgttgctctg ctagacgatt gacgagcagg
     8821 aaaaatgttg gtaatggaat atagatcacg agtaatgaaa gaaaaaagta aacaataaat
     8881 ggcatactat tcctcctaat tgtgtgagtt cgccctcatt ttaaaataac gatatttgtt
     8941 tgtgaagctt tttcgtgtcg taacaaatgt tatctaatgt atttttaaat aaataatcat
     9001 tattatcaaa ataatgttaa aaataaaaaa gaattgtcca tcactgttat tcaaatcatt
     9061 ttatcaaaac tacatagtaa atgttaaaaa gcaacatgac ataatatata gaaggcttta
     9121 cagtatatat gggagcttac taaaaaagaa tggagtaaga gttaacacaa tgggccatta
     9181 gctgaagttt taagcacttc aaggtatgaa acagaatggc ctgtaatact acatgattat
     9241 ttagactgct caaactgcta tcagtctggt ggaatgaata aagtattata ctaaaagcag
     9301 atccaactac aacgtaacag gaaaccagtt gtatcaggaa aagtgatagt acatggagaa
     9361 gaaaaggccc cctttgtatg agtcagtaca tatcttattt atagataaaa cggtattcaa
     9421 tcgaaataaa aaatc
//
LOCUS       DANKBF010000097         9191 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000097 DANKBF010000000
VERSION     DANKBF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..1369)
                     /locus_tag="PWY89_002641"
     CDS             complement(<1..1369)
                     /locus_tag="PWY89_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2067628.1"
                     /translation="MTEKNIMVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNS
                     SYEVQVSHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVIT
                     KSISRFARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQS
                     LSQNVKLGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQS
                     LVGIGRALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKN
                     EGHVPQYYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFC
                     GDCGDIYRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLA
                     GGNNMIKTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDE
                     LRDKRQ"
     gene            complement(1371..1787)
                     /locus_tag="PWY89_002642"
     CDS             complement(1371..1787)
                     /locus_tag="PWY89_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDL2067629.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            complement(1788..2285)
                     /locus_tag="PWY89_002643"
     CDS             complement(1788..2285)
                     /locus_tag="PWY89_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HDL2067630.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            complement(2596..3273)
                     /locus_tag="PWY89_002644"
                     /pseudo
     CDS             complement(2596..3273)
                     /locus_tag="PWY89_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(3273..3890)
                     /locus_tag="PWY89_002645"
     CDS             complement(3273..3890)
                     /locus_tag="PWY89_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2067631.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(3904..4074)
                     /locus_tag="PWY89_002646"
     CDS             complement(3904..4074)
                     /locus_tag="PWY89_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067632.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(4422..5912)
                     /locus_tag="PWY89_002647"
     CDS             complement(4422..5912)
                     /locus_tag="PWY89_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2067633.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(5955..6254)
                     /locus_tag="PWY89_002648"
     CDS             complement(5955..6254)
                     /locus_tag="PWY89_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067634.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(6620..6904)
                     /locus_tag="PWY89_002649"
     CDS             complement(6620..6904)
                     /locus_tag="PWY89_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067635.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(7423..7908)
                     /gene="vanZ"
                     /locus_tag="PWY89_002650"
     CDS             complement(7423..7908)
                     /gene="vanZ"
                     /locus_tag="PWY89_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDL2067636.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(8061..8972)
                     /locus_tag="PWY89_002651"
     CDS             complement(8061..8972)
                     /locus_tag="PWY89_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDL2067637.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2593 a         1819 c         1411 g         3368 t
ORIGIN      
        1 tctgtcgctt gtctctcagc tcatcaatct catctgctaa gtggtcatag tcttgaccct
       61 tgttcgccag cttgattagt tctttttgtt tttcctctag tgagttgtta atctctgaaa
      121 tttggtattc tgtggtttcg ccaatcacag catgaatatt ctcttccagt gtctttatca
      181 tattgttccc acctgcaagt agcttattaa tcgcagtcat tactgcacca taaagttcat
      241 cttcttttac ggttcggttc ttacaaactt caggaccttg ctcgattcta gtcacgcatc
      301 gccagacaaa ttcttttctt ccgtggatat tccaatagac ccgtctataa atatcgccac
      361 aatctccaca gaaggtgatg gcacttaaag cgtacttgct actgtaaatt cttttgttct
      421 tgccttctcc tgtgtagata ttgcttctcc gatgaatttc ttcttgcgcc tgcaaaaata
      481 actctttagg aataatcgct tcatggctat tttcaacata atattgggga acatgccctt
      541 catttttcac tcgtttcttg gtcagaaaat ctactgtgac agtcttttgc agaagggcat
      601 ctccgatgta tttttcgttt tgaagtatct tcttaactga ttctggtcgc catcttggtt
      661 ttcccgctgc tgttaaaata ccatcctttt caagggctcg accaatgccc actagactct
      721 gaccctcaag gtattctcgg tagatgcgtt tgataatctc agcttcttcg gggacaatag
      781 ttaaatttcc atcttcatct ttcgtgtagc ccataaatcg cttatggttg acctgcacct
      841 ttccttgttg gtatcggtac tgtagcccaa gcttaacgtt ttgtgaaagg ctctggcttt
      901 cctgctgtgc caaagatgcc ataatagtca gcaaaacctc acctttggca tccgtggtgt
      961 tgatgttctc tttctcaaaa taaactgata tgttcttatc cttgagctgt ctaatgtatt
     1021 gaaggcagtc tagggtgtta cgcgcaaatc ggctgatgga ttttgtaata accatgtcga
     1081 tgttaccgtc catacactct gcaatcatgc gattaaattc gtcacgcttt ttagtgttcg
     1141 taccagagat accatcatct gcaaagatgc cagcaaactc ccattcatta tttttcttta
     1201 taaactctgt atagtgtgag acctgaacct catagctaga attttgttct tctgtttctg
     1261 tagaaacacg gcaataggca gcaacacgaa gtttctttat cttttctttt gcggctgtac
     1321 ttcctactct tttacgagca ggaataacca ttatattttt ctctgtcact ttattcctcg
     1381 ctttctatca gactgtataa gtattctgct cgttcaaaag gatccactgg catcttatta
     1441 tctgcctttt tcattttgaa tcgttctttg ggagggggag aggtgaaagc ggcaagctct
     1501 accactcgtc ctaaatcctt tgcacgttta tctctaactt cttcagcttt atcaaatatc
     1561 tctttatcaa taattgctgg atacgtatca tttccaaggt agttgacgtt tttcaaaatg
     1621 cgccccatca cagaatgtgt cttttcaata cctgcctgtt cgccagccac tgtaagggat
     1681 agtcctgata tgtatttctc aaagaatacc tttacttgac ctgctgcctt ttcatcgaca
     1741 gtaaccactc catcttgaat tttgtatcca tatggaatat atgccattta tctcaccacc
     1801 ttttctttca gggaaagacc gcatttcaaa ttgaatgtca gctcatccct ggaatttaca
     1861 atgatgttca ctacaaattc ttcaaatagt tcttccgtgt agtcaccatt aaaattatct
     1921 gctgacacat aattaatgag gtcctttatc tcgtttgctc gcaaaacccc actcgcagag
     1981 ttcgttacca ggtttgtttt ttcggtagta agatttttaa tctcactatc taaaacattt
     2041 cgttcctgat taaaaagagc aggttctagg aaacctttgg acatcagtgc aataagggta
     2101 ttgcgttctt ccaatagttg ctccattagc ttatcaatag cgtccattct ttcacagtcg
     2161 ctttcttcat cgatttggct aactgacttg aaaagtggtt ctaggattag cttatgactg
     2221 aaagcaagct tattcatcat ggttgtcaat gtggctttga tttccccatc tcgcaaaaac
     2281 aacatggagc aactttcttt atcttcgata ctatctcaac atgtgtcgtg gataaggtaa
     2341 gtatgtaaaa tggtatgtgg aacatagtga tttgctgtaa atgatttgct aactacttta
     2401 gttctttata caaagtataa atcggatatt ttattatttt ttcttttata tcttctgaga
     2461 ttaggtcaaa gtatttgtcc gctaaatcag aaaggggttc ttttttcatc acctaatggg
     2521 tgatgaagca gaatacttta aagactatta aatcaatgtt aggaaagact attagtaaaa
     2581 ctatgaatta ttcaggaaaa aaggttcttt tttaaagttt tctattgctt cctggcgttg
     2641 ataaatgaga tataaagtcg gtgtttgtac acgtcctaat gaaaatgtac cttgtacgcc
     2701 tttttgttgt aaattcaagg tatataaagg gcttgcgttc atgccgatca accaatcggc
     2761 aatttggcgt gtttgcgctt cttgataaaa gggatagtaa ttcattcctg gttgcaaatt
     2821 ttgaaaaccg cttcggatca catctttttc taagctattg atccataatc ttttatacgt
     2881 tttatcttta gaaaaggcat ttgctttatg gataatcgac caggcgatat tttcaccttc
     2941 tctgtcgcta tctgttgcga caataattgt atttgccttt ttgagaagtt cagcaacaat
     3001 tttaaactgc tttcccttat cttttgcaac ttcaaaatcg tatcgatcag gaaaaatcgg
     3061 caaagattca agtttccaat tttgccactt ttcgtcataa tgacctggtt ctgctaattc
     3121 cactaaatgc ccaaaaccaa aggtgataaa cgtttcatct gtaaatagtg ggtctttgat
     3181 ctcaaaataa ccgtcttttt tggtgctttg ttttaaagca cttgcgtagg ctaatgcctg
     3241 gcttggtttt tcagctaaaa taaccgtact cattaactat ccctcttttc attgtttttt
     3301 ctttgatcga ctgtcacgtt atatcttgct cgataccttc taaacgttcg gcgattgatt
     3361 ccagtttgtt cttcaacttc tttatcggat aaaccattca aaaacaaatc gaaagcatgc
     3421 tttaaacgtg gatcattttc tttaaattta tgctgacgac ctttgaattt gccttttttc
     3481 ttagcaattt cgattccttg tgcctgacgt tccttaattt tttttcgttc tgattctgct
     3541 tgatacttgt acaattcaat gacaaggcta ttaatcaaac gccttaaatt ttcatcttca
     3601 ataccattca ttgagggtaa atttaagact tccagggttg cccccttaat ttgaatttga
     3661 ttcatcaatt ctgttaattc tttattattt cgtcctaatc gatctaattc agtaacaata
     3721 acaatatccc cttcacgaat atagttaagc atagcttgta attgtgggcg ttcgaccgat
     3781 tgaccgctta atttgtctga aaagacctta gaaacgccct gtaacgcttg taattgccga
     3841 tctaagttct gttctttgct actgacacgt gcataaccaa ttttagccat tttcaaccaa
     3901 cctctaaaat tctctcggtt gcaataacca atcagcaata tctacttttt caatttcaaa
     3961 ttgcttatca gaaattgtct tttcgtaagc gataaaatct tgcgcatatt gttgctcatt
     4021 aaaaatagcc accacttcgt cattttctaa aactcgataa ataaattttt tcattttact
     4081 cctcctatta tgcccaactt aaatgaccta ttcaccaagt caattatact gctaaaatca
     4141 tattaggaca aataggtata ctctaatgac ctataaaaga tagaaaatta aaaaatcaag
     4201 tgttcgcttc gctctcactg cccctcgacg ttttagtagc ctttccctca cttcgttcag
     4261 tccaagccaa ctaaaagttt tcgggctact ctctccttct ccccctaata attaattaaa
     4321 atcttactct gtatatttct gctaatcatt cgctaaacag caaagaaaaa acaaacacgt
     4381 atcatagata taaatgtaat ggcatagtgc gggttttatt ttcagcctgt atcatagcta
     4441 aacaaatcga gttgtgtgtc cgttttaggg cgttctgcta gcttgtttaa agtctcttga
     4501 atgaatgtat gctctaagtc aaaagaattt gtcagcgcct ttatatagct ttctttttct
     4561 tcttttttta ctttaatgat cgatagcaac aatgatttaa cactagcaag ttgaatgcca
     4621 ccatttcttc ctggtttaat cttaaagaaa atttcctgat tcgccttcag taccttcagc
     4681 aatttatcta atgtccgttc aggaatgcct agcacttctc taatctcttt tttggtcgtc
     4741 actaaataag gcttgtatac atcgcttttt tcgctaatat aagccattaa atcttctttc
     4801 cattctgaca aatgaacacg ttgacgttcg cttctttttt tcttgaattt aaaccaccct
     4861 tgacggacaa ataaatcttt actggttaaa tcacttgata cccaagcttt gcaaagaatg
     4921 gtaatgtatt ccctattagc cccttgatag ttttctgaat aggcacttct aacaattttg
     4981 attacttctt tttcttctaa gggttgatct aatcgattat taaactcaaa catattatat
     5041 tcgcacgttt cgattgaata gcctgaacta aagtaggcta aagagagggt aaacatgacg
     5101 ttattacgcc ctattaaacc cttttctcct gaaaatttcg tttcgtgcaa taagagatta
     5161 aaccagggtt catctacttg ttttttgcct tctgtaccgc ttaaaaccgt tagacttgaa
     5221 cgagtaaagc ccttattatc tgtttgtttg aaagaccaat cttgccattc tttgaaagaa
     5281 taacggtaat taggatcaaa aaattctaca ttgtccgttc ttggtatgcg agcaatacca
     5341 aaatgattac acgttagatc aactggcaaa gactttccaa aatattctcg gatattttgc
     5401 gaaattattt tggctgcttt gacagattta aattctgatt ttgaagtcac atagactggc
     5461 gtttctaaaa caaaatatgc ttgataacct ttatcagatt tgataatcat agtaggcata
     5521 aaacctaaat caatagcggt tgttaaaata tcgcttgctg aaatagtttc ttttgccgtg
     5581 tgaatatcaa aatcaataaa gaaggtattg atttgtctta aattgttttc agaatgtcct
     5641 ttcgtgtatg aacggttttc gtctgcatac gttccataac gataaacgtt gggtgtccaa
     5701 tgtgtaaatg tatcttgatt ttcttgaatc gcttcctcgg aagtcagaac aacaccacga
     5761 ccgccaatca tgcttgattt tgagcgatac gcaaaaatag cccctttgct tttacctggc
     5821 ttggtagtga ttgagcgaat tttactattt ttaaatttgt actttaacaa gccgtcatga
     5881 agcacagttt ctacaacaaa agggatattc attcagctgt tctcctttcc tataaatcct
     5941 ataaaatagg ttgtttaatt aacttggttt gctttttcat tcaactgttt caatattgca
     6001 tgttttgaaa aagatttttt tcctttataa gtcaattttt ttccactaat cgaataaatt
     6061 attttgttat tttctattaa cttatatata taatcttccc cctccgaaga aaaatactta
     6121 tctgattttg tttctaagta gatatttctc ttttctaact ctttcttaaa cgtttctagt
     6181 gtatagatat ttgctaattt tcttatctcc aataaactat tttttatata agttttacat
     6241 tcatcatgat tcatacaaac tccaccttct ataaatgaat acaaaaaaag caatcaaacg
     6301 atttccgatt gattgcttaa caattcttaa attcagtagc ttagatactt gaaaactctc
     6361 tgatttccct atataatgat agtacggtta tataccgtct tcaaacaaag ttaattaaat
     6421 aacttcttac gagggaagag ttcatctgac taactgataa gcgttggttt ggcaatctta
     6481 tcgggctatg catttataaa atgtcgtcaa acattttata aatgtgtcat ggctcttttt
     6541 tcgtttctat tcagttcgtt gtttcgttat atctagtata ccgcttttaa aaaaaataag
     6601 caacgatttc gtgcattatt cacacgaagt cattgctttt ttcttcttcc atttctaaat
     6661 ccaatgttac ttgttctgat tctgtttctg gttctggttc tgttggctca tttgggatta
     6721 aatccactac tagcgttgag ttagttaact ttgcaatttg ttctagtgtt tttatggttg
     6781 gatctgattt tcctgattct attcgtgaat aatttgatct actcatttct aattcttggg
     6841 agaatttttc ttttgatttg gtacctctca tcttttttaa gctttctcta aatgctagtt
     6901 ccaagtttct cccccctctt tttgtgtaat aaaattcacg ctttatttaa attatagctt
     6961 ttaatatctt aataaacaag tgattaatca cttgtgattt gtgattattc caaaagtagg
     7021 taatttcacc aacctttttc aaaagttttt atcgacgatt attggactgg atcgtgtttc
     7081 ctaaagcgat tctaagcgca tgtttaattt taaagtgggt aattctcttt aaaacgtcct
     7141 acaaagattc aaaaaaagca tggctgctag tgggggtacc gtcaggaaaa tgcggattta
     7201 caacgttaag gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc
     7261 tttctttgtt actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact
     7321 tcacacctac gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata
     7381 tctttatgtg tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct
     7441 taaatgggta cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac
     7501 tctgtctaat tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag
     7561 aaagcctcca acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat
     7621 aaattgaatt atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa
     7681 tccgatttct ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt
     7741 atctatcatc tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg
     7801 atgataatta aatactgata aaatattgta ttgtaatttg aataacacta accagattag
     7861 tgtcactaaa tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc
     7921 taattctttt gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta
     7981 aattaaaaag ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg
     8041 ttggtttcat tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact
     8101 gtcacaatta caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca
     8161 tgaatggtgg tatttttagt aacaggataa taaaagatct catatttttc cccatttaca
     8221 ctaacagaaa tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc
     8281 ttttctttca taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat
     8341 tgaattcctg ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa
     8401 gcattttctt ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct
     8461 acatctagtg ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc
     8521 cccatttctt ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata
     8581 aaatgactaa cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt
     8641 tcttttgaca tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt
     8701 ttagataaat tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg
     8761 attaatagca gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt
     8821 tcagaagtcc cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg
     8881 actttttcct gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat
     8941 aacaataaca ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt
     9001 ggaagtatga aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt
     9061 gccgttttgc cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac
     9121 caatgtagat gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct
     9181 gttgacaatg g
//
LOCUS       DANKBF010000098         8942 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000098 DANKBF010000000
VERSION     DANKBF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            51..260
                     /locus_tag="PWY89_002652"
     CDS             51..260
                     /locus_tag="PWY89_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067638.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="PWY89_002653"
                     /pseudo
     CDS             467..667
                     /locus_tag="PWY89_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="PWY89_002654"
     CDS             complement(1379..1984)
                     /locus_tag="PWY89_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2067639.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="PWY89_002655"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="PWY89_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="PWY89_002656"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="PWY89_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="PWY89_002657"
     CDS             3019..3990
                     /locus_tag="PWY89_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2067640.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="PWY89_002658"
     CDS             4130..5443
                     /locus_tag="PWY89_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2067641.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="PWY89_002659"
     CDS             5486..6352
                     /locus_tag="PWY89_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2067642.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="PWY89_002660"
     CDS             6349..7197
                     /locus_tag="PWY89_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2067643.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="PWY89_002661"
     CDS             7211..8683
                     /locus_tag="PWY89_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL2067644.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="PWY89_002662"
     CDS             8700..>8942
                     /locus_tag="PWY89_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2067645.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DANKBF010000099         8552 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000099 DANKBF010000000
VERSION     DANKBF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..687
                     /locus_tag="PWY89_002663"
     CDS             <1..687
                     /locus_tag="PWY89_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2067646.1"
                     /translation="LDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIKRVE
                     QIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIYPTA
                     LKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINE
                     KKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLN
                     EESSDERKQI"
     gene            668..1402
                     /locus_tag="PWY89_002664"
     CDS             668..1402
                     /locus_tag="PWY89_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2067647.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1435..2343
                     /locus_tag="PWY89_002665"
     CDS             1435..2343
                     /locus_tag="PWY89_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2067648.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2340..2882
                     /gene="sat4"
                     /locus_tag="PWY89_002666"
     CDS             2340..2882
                     /gene="sat4"
                     /locus_tag="PWY89_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL2067649.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2975..3769
                     /locus_tag="PWY89_002667"
                     /note="aph(3')-IIIa"
     CDS             2975..3769
                     /locus_tag="PWY89_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL2067650.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4045..4617
                     /locus_tag="PWY89_002668"
     CDS             4045..4617
                     /locus_tag="PWY89_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2067651.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            4915..5811
                     /locus_tag="PWY89_002669"
     CDS             4915..5811
                     /locus_tag="PWY89_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2067652.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            5910..6119
                     /locus_tag="PWY89_002670"
     CDS             5910..6119
                     /locus_tag="PWY89_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2067653.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            6136..6408
                     /locus_tag="PWY89_002671"
     CDS             6136..6408
                     /locus_tag="PWY89_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2067654.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            6410..7273
                     /locus_tag="PWY89_002672"
     CDS             6410..7273
                     /locus_tag="PWY89_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2067655.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(7536..8273)
                     /gene="erm(B)"
                     /locus_tag="PWY89_002673"
     CDS             complement(7536..8273)
                     /gene="erm(B)"
                     /locus_tag="PWY89_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2067656.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(8398..8481)
                     /locus_tag="PWY89_002674"
     CDS             complement(8398..8481)
                     /locus_tag="PWY89_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2067657.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         3005 a         1299 c         1751 g         2497 t
ORIGIN      
        1 ttggatgatg agaatagaaa caaccttgtt gtacctcccg gagcgtgggg tgattgggtt
       61 aatggcggtg gatggttagt tataaacggg tgtcatgttg acttgatatt acgtgatata
      121 aaacgggtgg aacaaataat caaagatacg gagcaaggaa ttgttactgc caattatcag
      181 accgggcatc cccatggtta tataagtgct atgtatcgtg gagaattagc gattagtaaa
      241 atactatatg ctaagaacga aagcttatgc gaattaaaaa agcaggcaga aatttaccct
      301 actgctctaa agaaaagctt gatgaacttt tttatatttg aagctgaatt ctctttaatg
      361 tttgtaaaag caaatgcggg ggcagaggat aaatattata ttgcaggcca tgtttttcgt
      421 attatttcat gcttaaatca agtactattt gcatgtaata atgcttattg cattaacgaa
      481 aagaaagcta taaaactgct tgaaactttt gaatataaac ctgaaaaata tgccgaaaga
      541 gttaatcata tttttgaagt actcggtctt tctctttttg aatgctacga catgaccgag
      601 aaactttata aagaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag
      661 agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat
      721 gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct
      781 acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat
      841 atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct
      901 cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga
      961 agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta
     1021 tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa
     1081 tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta
     1141 taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg
     1201 gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct
     1261 aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga
     1321 gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt
     1381 atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga
     1441 tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga acgtattcga
     1501 attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga atttcaggat
     1561 tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga tgactggctt
     1621 aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt attcccacct
     1681 gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat tgatcttacc
     1741 ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat aaaggttcta
     1801 attgataaag attgtagaat taaaagggac atagttccga ctgatataga ttatcatgta
     1861 agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa tgtaacacct
     1921 tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca ttttaatcag
     1981 attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga aacaggcttt
     2041 aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga ggatttgtgg
     2101 gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga agcattattt
     2161 ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc
     2221 tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa aaaatacact
     2281 ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag gcgggaacag
     2341 tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc gaaccatttg
     2401 aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt acagaattac
     2461 tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag gatgaggagg
     2521 cagattgcct tgaatatatt gacaatactg ataagataat atatctttac taccaagacg
     2581 ataaatgcgt cggaaaagtt aaactgcgaa aaaattggaa ccggtacgct tatatagaag
     2641 atatcgccgt atgtaaggat ttcagggggc aaggcatagg cagcgcgctt atcaatatat
     2701 ctatagaatg ggcaaagcat aaaaacttgc atggactaat gcttgaaacc caggacaata
     2761 accttatagc ttgtaaattc tatcataatt gtggtttcaa aatcggctcc gtcgatacta
     2821 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     2881 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     2941 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     3001 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     3061 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     3121 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     3181 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     3241 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     3301 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     3361 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
     3421 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     3481 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     3541 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     3601 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     3661 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     3721 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctagattt
     3781 agatgtctaa aaagctttaa ctacaagctt tttagacatc taatcttttc tgaagtacat
     3841 ccgcaactgt ccatactctg atgttttata tcttttctaa aagttcgcta gatagagttc
     3901 tatattagag atgtaaagag ttttggtgaa gaggtttgtg agtatggctt ttataaccag
     3961 ggtgcaatga gaagcactaa cagcagctag cttaagaacg cataggatat actttttatg
     4021 gaagtcacag aggaggaata aaatttgaac aggattaaca gagtaacctc tattcttatt
     4081 cagctgcaat caaaaaaaat aattcctgcc aaagaaattg cacagcgatt taatataagc
     4141 ttaaggacag tttacagaga tatccggaca cttgaagaag ccggaatacc aattggatct
     4201 gaggccggaa aaggatattt tcttgtagag ggcttcctac ttccgccggt aatgtttaca
     4261 gcggcggaag tgggtgcatt gattacagca gggaaatttt taaattgcca tggagatgaa
     4321 tcatttataa aggattttga ttcagctatg tataaaatca aatctatttt gaagcatggt
     4381 gaaaaaaact atgcacagga gttggagaac agtattaacg tgtacagcac atctggacag
     4441 aaaaatacat tggcggataa cgttattgca gcaattcaga ctgcaatctg caataaaagg
     4501 gtaatatcaa ttcaatatcc tgcatcagga gggcaagaac cggaaagcag aatgatagag
     4561 cctatatcac tggggtttta tgaacagaac tggtacttaa tcggttttgc aggctaagaa
     4621 atgaatatag gaattttcgt gtggatcgga ttaaaagcat ttgcatagaa gaagagctct
     4681 gtcaaagcca tgatggctct ttggaacaaa tattaaaaca aatgctttca tataaaaaac
     4741 tatatgccct acaatgccgt tgtaaaaatt ggggaaaagg gatatattga ttttatatct
     4801 ttctcaaaat aaatataaaa gccctttaaa gagggctttt atatattaat cacaaatcac
     4861 ttatcacaaa tcacaagtga ttaatcacaa atcacttgtg atttgtgatt cttaatgata
     4921 caatattact atacaaaaaa agaatggggc gtagttatgg agaaggaaaa actaaaaata
     4981 cttgaagaat taagacgtat tttaaacaat aaaaatgaag caattattat cttgaataat
     5041 tactttaaag gtggtgttgg aaagtccaaa ttatcgacta tgtttgctta cttgacagac
     5101 aaattgaatt taaaagtttt aatgatcgat aaggacttac aggcaacatt gacaaaagac
     5161 ttagcaaaaa cttttgaggt agaattgcca cgtgtcaatt tttatgaagg cttgaaaaat
     5221 ggaaacttgg cttcttctat tattcatttg actgataatt tagacttgat ccctggcacg
     5281 tttgatttga tgttactgcc aaaattaact cgctcatgga cgtttgaaaa tgaaagtaga
     5341 ttgcttgcta ctcttttagc acctttaaaa agtgactatg atcttattat tattgatact
     5401 gtaccaacgc caagcgttta tacaaataat gcaatcgtgg cgagtgatta cgttatgatc
     5461 cctttacaag cagaagaaga aagtacaaac aacattcaaa actatatttc ctatttgatt
     5521 gatttgcaag aacagtttaa ccctggacta gatatgattg gttttgttcc ttatttagtt
     5581 gatacggaca gcgcaacgat aaaatcaaac ctggaagaac tgtacaaaga acataaagag
     5641 gataatttgg ttttccgaaa tattatcaag cgaagtaata aagtaagtac ttggtctaaa
     5701 aatggcatta cagaacacaa aggctatgac aaaaaagttt tgtctatgta tgagaacgta
     5761 ttttttgaaa tgattgagcg aatcattcaa ttagaaaatg aaaaagaata gaatcacaaa
     5821 tcacaagtga ttaatcacaa atcacttgtg atttgtgata ggtgatgata aaataaatag
     5881 taagaagaaa tagaaagaag tgagcgatcg tgggaaattt aggcgcacag aaagcaaaac
     5941 gaaatgatac gccaatcagc gcaaaaaaag atataatggg ggataagacg gttcgtgttc
     6001 gtgccgactt gcaccatatc ataaaaatcg aaacagcaaa gaatggcgga aacgtaaaag
     6061 aagttatgga tcaagcctta gaagaatata tacggaaata tttacctgac aaactttaaa
     6121 aggagtgaaa atgaaatggc agttacgtat gaaaaaacat ttgaaattga aatcattaat
     6181 gagttatcgg caagtgttta taatcgggta ttaaattatg ttttgaatca tgaactagat
     6241 actaaaaata ctcgtttact agaagtgaat cttttaaatc aattagaagt ggcacaagaa
     6301 gttgatttat ttcaacaacc atttgaagaa ttacaagcta ttcatgagta ttggcggtca
     6361 atgaatcaat attcaaaaca aattttgaat aaagagaaag tggcttaaca tggcgaatat
     6421 tactgacttc accgaaaagc aatttgaaga tcgtttagaa aagaatgttg aacgactaac
     6481 taaaaataga ctagcggttg aatcgccaac cgctttttta cttggtgggc aaccagggtc
     6541 agggaaaacc agtttgcgat cagcaatttt tgaagaaaca caagggaatg ttattgtcat
     6601 tgataatgat acctttaaac aacagcaccc aaattttgac gaattagtga aactctatga
     6661 aaaagacgta gtaaaacacg ttacccctta ttcgaatcgc atgacagaag cacttattag
     6721 ccgtttaagc gatcaaggct ataatttagt gattgaagga acaggacgaa caacagacgt
     6781 tcccattcaa acagccacaa tgcttcaatc taaaggttat gaaacaaaaa tatacgtcat
     6841 ggcagtaccg aaaattgaat catacttagg aacgattgaa cgatatgaaa ccatgtatgc
     6901 agatgatcca atgacagcta gggcaacacc aaaacaagcg catgatattg ttgtcaaaaa
     6961 cttaccgacc aatttagaaa cccttcataa aacgggctta tttagcgata taaggcttta
     7021 caatagagaa ggagtgaaac tctattcaag cttagaaacg ccttccatta gtccgaaaga
     7081 gaccttagaa agagaattaa atcgtaaagt atcagggaaa gaaattcaac cgactttgga
     7141 gagaatagag caaaaaatgg ttcaaaatca acaccaagaa acccctgaat ttaaagcaat
     7201 tcaacaaaaa atggaaagct tgcagctacc tacaccacca atacccaaaa cacctaaact
     7261 tccaggtatt taaaccttaa aaaaggaaaa gagtagttac caaaaaacgg taactactct
     7321 ttttttataa aaacatttcc tcatgttggg gtcccgagcg cctacgagga atttgtatcg
     7381 ataagaaata gatttaaaaa tttcgctgtt tttttctaat ttcattttga ggtgaattaa
     7441 ttggtagtta ttagaggtgc cctataccta ataatttatc tacattccct ttagtaacgt
     7501 gtaactttcc aaatttacaa aagcgactca tagaattatt tcctcccgtt aaataataga
     7561 taactattaa aaatagacaa tacttgctca taagtaacgg tacttaaatt gtttactttg
     7621 gcgtgtttca ttgcttgatg aaactgattt ttagtaaaca gttgacgata ttctcgattg
     7681 acccattttg aaacaaagta cgtatatagc ttccaatatt tatctggaac atctgtggta
     7741 tggcgggtaa gttttattaa gacactgttt acttttggtt taggatgaaa gcattccgct
     7801 ggcagcttaa gcaattgctg aatcgagact tgagtgtgca agagcaaccc tagtgttcgg
     7861 tgaatatcca aggtacgctt gtagaatcct tcttcaacaa tcagatagat gtcagacgca
     7921 tggctttcaa aaaccacttt tttaataatt tgtgtgctta aatggtaagg aatattccca
     7981 acaattttat acctctgttt gttagggaat tgaaactgta gaatatcttg gtgaattaaa
     8041 gtgacacgag tgttcagttt taatttttct gacgataagt tgaatagatg actgtctaat
     8101 tcaatagacg ttacctgttt acttatttta gccagtttcg tcgttaaatg ccctttacct
     8161 gttccaattt cgtaaacggt atcggtttct tttaaattca attgttttat tatttggttg
     8221 agtacttttt cactcgttaa aaagttttga gaatatttta tatttttgtt catgtaatca
     8281 ctcctgaagt gattacatct ataaataaat acagaagtta aacgatttgt ttgtaatttt
     8341 agttatctgt ttaaaaagtc ataagattag tcactggtag gaattaatct aacgtattta
     8401 tttatctgcg taatcactgt ttttagtctg tttcaaaacg gtagatgttt tatctacatt
     8461 acgcatttgg aataccaaca tgacgaatcc ctccttctta attacaaatt tttagcatct
     8521 aatttaactt caattcctat tatacaaaat tt
//
LOCUS       DANKBF010000100         8494 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000100 DANKBF010000000
VERSION     DANKBF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..173)
                     /locus_tag="PWY89_002675"
     CDS             complement(<1..173)
                     /locus_tag="PWY89_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2067658.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PWY89_002676"
     CDS             complement(593..1714)
                     /locus_tag="PWY89_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL2067659.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PWY89_002677"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PWY89_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL2067660.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PWY89_002678"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PWY89_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL2067661.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PWY89_002679"
     CDS             complement(4304..5215)
                     /locus_tag="PWY89_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2067662.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PWY89_002680"
     CDS             complement(5232..6239)
                     /locus_tag="PWY89_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2067663.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PWY89_002681"
     CDS             complement(6236..8365)
                     /locus_tag="PWY89_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2067664.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="PWY89_002682"
     CDS             complement(8370..>8494)
                     /locus_tag="PWY89_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2067665.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DANKBF010000101         8248 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000101 DANKBF010000000
VERSION     DANKBF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            234..1559
                     /locus_tag="PWY89_002683"
     CDS             234..1559
                     /locus_tag="PWY89_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2067666.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="PWY89_002684"
     CDS             1552..1902
                     /locus_tag="PWY89_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067667.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1884..2069
                     /locus_tag="PWY89_002685"
     CDS             1884..2069
                     /locus_tag="PWY89_002685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067668.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /locus_tag="PWY89_002686"
     CDS             complement(2973..3248)
                     /locus_tag="PWY89_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL2067669.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="PWY89_002687"
     CDS             complement(3220..4173)
                     /locus_tag="PWY89_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2067670.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="PWY89_002688"
     CDS             4785..6278
                     /locus_tag="PWY89_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2067671.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="PWY89_002689"
     CDS             6413..6709
                     /locus_tag="PWY89_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2067672.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="PWY89_002690"
     CDS             6810..7661
                     /locus_tag="PWY89_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067673.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7965
                     /locus_tag="PWY89_002691"
                     /pseudo
     CDS             7682..7965
                     /locus_tag="PWY89_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2863 a         1283 c         1552 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DANKBF010000102         7601 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000102 DANKBF010000000
VERSION     DANKBF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(943..1356)
                     /locus_tag="PWY89_002692"
     CDS             complement(943..1356)
                     /locus_tag="PWY89_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL2067674.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="PWY89_002693"
     CDS             complement(1812..2297)
                     /locus_tag="PWY89_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067675.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="PWY89_002694"
     CDS             complement(2436..2651)
                     /locus_tag="PWY89_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL2067676.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="PWY89_002695"
     CDS             2875..3675
                     /locus_tag="PWY89_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2067677.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="PWY89_002696"
     CDS             3694..5562
                     /locus_tag="PWY89_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2067678.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="PWY89_002697"
     CDS             5555..6046
                     /locus_tag="PWY89_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2067679.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="PWY89_002698"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="PWY89_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2067680.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="PWY89_002699"
     CDS             6606..7601
                     /locus_tag="PWY89_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2067681.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DANKBF010000103         7537 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000103 DANKBF010000000
VERSION     DANKBF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            107..1248
                     /locus_tag="PWY89_002700"
     CDS             join(107..335,335..1248)
                     /locus_tag="PWY89_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2067682.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="PWY89_002701"
     CDS             1483..1935
                     /locus_tag="PWY89_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067683.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="PWY89_002702"
     CDS             1949..2638
                     /locus_tag="PWY89_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067684.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="PWY89_002703"
     CDS             2763..5114
                     /locus_tag="PWY89_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL2067685.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="PWY89_002704"
     CDS             5194..5589
                     /locus_tag="PWY89_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067686.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="PWY89_002705"
     CDS             5914..6531
                     /locus_tag="PWY89_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2067687.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="PWY89_002706"
     CDS             6572..6871
                     /locus_tag="PWY89_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL2067688.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="PWY89_002707"
     CDS             6905..7072
                     /locus_tag="PWY89_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2067689.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="PWY89_002708"
     CDS             complement(7117..>7537)
                     /locus_tag="PWY89_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2067690.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DANKBF010000104         7217 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000104 DANKBF010000000
VERSION     DANKBF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="PWY89_002709"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="PWY89_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL2067691.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /locus_tag="PWY89_002710"
     CDS             complement(919..1677)
                     /locus_tag="PWY89_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2067692.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="PWY89_002711"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="PWY89_002711"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2067693.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="PWY89_002712"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="PWY89_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2067694.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="PWY89_002713"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="PWY89_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2067695.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="PWY89_002714"
     CDS             complement(4433..5290)
                     /locus_tag="PWY89_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2067696.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="PWY89_002715"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="PWY89_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2067697.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DANKBF010000105         7153 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000105 DANKBF010000000
VERSION     DANKBF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..1368)
                     /locus_tag="PWY89_002716"
     CDS             complement(join(<1..795,795..1368))
                     /locus_tag="PWY89_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HDL2067698.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIK"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="PWY89_002717"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="PWY89_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL2067699.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2331..2429
                     /locus_tag="PWY89_002718"
                     /pseudo
     CDS             2331..2429
                     /locus_tag="PWY89_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(3112..3930)
                     /locus_tag="PWY89_002719"
     CDS             complement(3112..3930)
                     /locus_tag="PWY89_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2067700.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4091..4780
                     /locus_tag="PWY89_002720"
     CDS             4091..4780
                     /locus_tag="PWY89_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2067701.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4794..6296
                     /locus_tag="PWY89_002721"
     CDS             4794..6296
                     /locus_tag="PWY89_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2067702.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6309..6785
                     /locus_tag="PWY89_002722"
     CDS             6309..6785
                     /locus_tag="PWY89_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2067703.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6882..6992)
                     /locus_tag="PWY89_002723"
                     /pseudo
     CDS             complement(6882..6992)
                     /locus_tag="PWY89_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2318 a         1131 c         1294 g         2410 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgatga tctgtctaac ttaatttgac aattaagagt atatttactt cagatggtaa
     2461 ggcaacaaaa taaaatacaa taggcagatg aactaataaa taaaatctgt attaaataca
     2521 gataaattaa tataaaaatc aaaaggatca taaactttta acaagtgatg aaatgggtgg
     2581 aaattgattc ttatagacag tttgtctcat aacattgaag tattttgcaa ttttagcatt
     2641 gaaacacttt tttaatcttc aacgttgttc ttataaacaa gatgattggg aatcttttga
     2701 attaacactg tatagcttag aacacccttt atagaaaaca ttcgcttagc aatcttaata
     2761 attccttgtg cccgatgtcg tttagattca gtaggttcct gctcatcgat catggaaaaa
     2821 atttgaagga tcagatcctt aataaattta tttaagagag gatgatccat tgcttcatcc
     2881 atactcgata aactagtgat aatgagctac acgtttttct ttcaagttga tcaatagctt
     2941 ccacgataaa aatatcttta gataaaatca agaaccgtag gaactacagg gatatttggt
     3001 caataagagc caccgctatt agtaaaaaat aagctatcaa gtatgctctc atcccaagaa
     3061 ttgcctactt caatcgttaa aaaccgttag gtttagctaa gtgggggtag ttcactcaat
     3121 acttttatca ataatagagc gcaaaacttt ttctcttaat tcaactgaat caatattatt
     3181 gttttgttca taaccatgaa ctaatagatc acaaatataa agttgtgata tttttccagc
     3241 taaagatcca ccattaagaa attcttcaat ggctgtttgt aaaactaagt ctgcagactt
     3301 tcctataggt gaatgaatat agttagttat agcaataatt tttgcgccat tttttttagc
     3361 tattttcaat gaatcataag tatctttcgt tttcccagat aaagaaaaaa tgattactaa
     3421 atcattaact gttaataaag aagcgacttg ggcttgaaaa tgagggtcta gtacagcttt
     3481 tgcttgaact cctacgcgaa gaaacatatt ttctaaatca agactagtat ttccactaga
     3541 tcccactcca aatatatata tgttttttga ttgagtgatc attttaatgg cttcattcaa
     3601 tttttcttcg tctaaaagcc taatagtgga atagagagct tctgttaaac tattagcaat
     3661 ttcatcatag tactttacac tagtcggatg ttcttttttt tgactaaagt cttcctttgc
     3721 aatttcaatt tttaaatcag aaaaaccaga aaaaccaatt ttttgacaaa agcgaataat
     3781 agtagcatcg ccaacttttg tgtttttctt aatatcactc atcgtactat aaataatagt
     3841 tttcccagaa tttaaaataa aatcagcgac ttttttttca gacttcgtta aaaatggata
     3901 attttcttta attaaataga tataactcat acaaactcct ctacaatttc aacttttctc
     3961 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
     4021 tgaaagcgga tgctatattg tttttggagt tattatccaa aaataataaa aaattggagc
     4081 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
     4141 atgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
     4201 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
     4261 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
     4321 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
     4381 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
     4441 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
     4501 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
     4561 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
     4621 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
     4681 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
     4741 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
     4801 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
     4861 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
     4921 ttttagatca accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     4981 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     5041 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     5101 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     5161 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     5221 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     5281 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     5341 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     5401 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     5461 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     5521 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     5581 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     5641 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     5701 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     5761 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     5821 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     5881 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     5941 tatatttctt tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg
     6001 aagaagaatc agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag
     6061 agtctcttat cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt
     6121 gtgcaactcg tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa
     6181 aagacactgg tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag
     6241 ggaaagctga cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg
     6301 tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt
     6361 ccggaaaaat catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag
     6421 gtgatggttt tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag
     6481 taacaaatat atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt
     6541 atttgattca tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg
     6601 ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa
     6661 taataaaatc tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca
     6721 atgatttaat aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt
     6781 tttaaaaaag tgtatataaa aagctataaa ataagctaaa taggatattt gtcaaatgaa
     6841 gttggtgaac agaaagaact gttttctttc tgttcatttt tttattttat tagtaatgat
     6901 tccccatgcc aaatcatgat ccgtaattta aagttataaa agcttcgaaa tccattggca
     6961 tttcgttgga ataccttgat atgattattc aaatataaaa tatagagaga agagagagag
     7021 ggaagatatt tataaagggc aaagttatgt ttactcaact taggaaatag aaatagatca
     7081 atttatattc tcttgtcaat cgaatatgaa ccttagcact atataccaaa tatagtaaaa
     7141 tgctgaaaga tgt
//
LOCUS       DANKBF010000106         6390 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000106 DANKBF010000000
VERSION     DANKBF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(120..1250)
                     /locus_tag="PWY89_002724"
     CDS             complement(120..1250)
                     /locus_tag="PWY89_002724"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL2067704.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1310..1537)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1899..3101)
                     /locus_tag="PWY89_002725"
     CDS             complement(1899..3101)
                     /locus_tag="PWY89_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2067705.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3161..3364)
                     /locus_tag="PWY89_002726"
     CDS             complement(3161..3364)
                     /locus_tag="PWY89_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2067706.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3506..4738)
                     /locus_tag="PWY89_002727"
                     /pseudo
     CDS             complement(3506..4738)
                     /locus_tag="PWY89_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4901..5713
                     /locus_tag="PWY89_002728"
     CDS             4901..5713
                     /locus_tag="PWY89_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2067707.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1914 a         1307 c         1006 g         2163 t
ORIGIN      
        1 gcttaatttg acaaataata caaataaata atataaaagg taaataaaaa agagtggatt
       61 tctccactcc atgaaaagcc gactttttgg gacgcttttt tttgccaagc ctcacatttt
      121 tatagccaaa cttcttttgc gacttgctgc aataattgga ttttcttcca ttgttcttct
      181 tctgttaaat ggtttccttc ttctgttgaa gcaaacccac attgaggact gatacttaga
      241 ttttccaatg gtacaaattg ttgtgcttct tttattcgtt cttttatttt ctccgtatct
      301 tccaaatctg gaaacttaga agtaactaat cctaaaacaa ctttactttc tttgttactc
      361 caatgtctta aaggagtaaa atctcctgct cggtcagaat cgtattccaa aaagaaacca
      421 tcataatttg ttgaaaacaa ctcttctgca atgggttcat atccaccgga aaacagccaa
      481 gaagaagcgt gattcccgcg gcagatatgt gttgtgatcg tcaaatcttc tggtttgtct
      541 tccagtgcgt tattgataat ctctgtacaa atatgggcca tttctttcgg atcaattcct
      601 ttttgaagaa gtgcttctct tttagtttga tcagctagaa acgcccaatt tgtatcatcg
      661 aattgcaggt aacggcaacc tttttcataa aaagcatgaa tcgctgcttt gtaagtagag
      721 gacaagtcct cataaaaatc agatagatca ggataaatct ccttgatttt tgaagaacca
      781 tcattagata ggatctcttg tcgtaaaatc atcgtgggac tagggatcgt tgcttttgca
      841 acaaaagtat catctgtttc cactacttct ttcagaaaag caaaatcttt taaaaatggg
      901 tgatttggat tgaaagataa tttatctact acccgaatat tataagctgg tgctgctttt
      961 ccgtggaatg tttgattata tccagtttct ggaacatagc cttcgatacc atttagttct
     1021 tctaaaaaat caagatgcca aaatccacgc cgaaattcac catctgtaat tcctttgatg
     1081 ccgactgctt tttggtcttc cactaatttt tttatttcct ggtcttcgat tgcacgaagt
     1141 tcttcttttg aaagtaagcc ttcttgaact ttttttcttg cttctttgat cgattgcggg
     1201 cgtaagaaac ttccgacatg atctactgta aatggtctat ttttgctcat ttcactcact
     1261 cttttctttg ttttttgtaa aaaaaaatcc ttttactagc aaatactagt aaaaggacga
     1321 ttatactcgt ggtaccacct tttttcagaa aaaacattct ttctctcagc ctgacaataa
     1381 aatcagtggt cttggtaacg aaagactgtt cgtttttgcc tacctttcga caaaaaatcc
     1441 tctaaggcca ttttcgacag tcaacttcta gctttttaca ccaaccaaag cctctcttga
     1501 accattttct gtctactttc cttctcatcg gatactatta gaataaaatt agaatagcat
     1561 gtttgaaaga aaagtcaata gggaataaga acaaacaaaa actaatttgt tattcttagt
     1621 aatttgtttg tggaatacaa taaagctttt ctaatatgat aattgttcag tcgtcctttc
     1681 atcttagatc acttccttaa aatgatcata cccgtttcaa ccacataaaa aaggacttaa
     1741 ttttgaaaaa tttcccatca aaactaagtc ctttttatta tagttatgca aatttgctta
     1801 ttttctcgct atttccgata catcttgaac aggagcaatt cgactgaaaa cactggtgca
     1861 aaagcattta tttgtcttat ggtagaaccg tggaaaactc agactggtaa taaattgtca
     1921 ttttttgaaa gaatagcgcc ctcttgaaca ttatcaatcg cacttaccac ttgttgatct
     1981 gactttgcat ataagtgacc gtaagttcct aaagtgattt gaatattcgc atggcccaat
     2041 cgttcttgaa tagatagcgg ttgaatattc tgctcaatta gaaatgcaac atgagaatgt
     2101 cgtagcgcgt gaagcttaat cggtttgaca cccgcttttt tagcagcttc ttttaaccag
     2161 ttcgcaaaag aataaggtgg cgtaaatgtt ccgtccaact gagcaacata ttcaaaattt
     2221 ccaatacgtt tttgcaagtc ataccactgt ctgagtttta aggaaagctt cttccccaac
     2281 ccaatcgttc gataagcaca tttagttttg ggagtcgaaa agtaccaatc atcttttgta
     2341 ttataaatca atgtcttctc aatcgttatc gtgtttttct caaaatctac attcgaccaa
     2401 gacaaagctt caagttctcc aatccgaatg ccagtataaa agagcatttc atacacaatc
     2461 ttccgatggt gggtttgaat cttatcatcc ttcaattcat tcatcacttt ttgaaactca
     2521 ctgtgttccc aaaactcaac tttcttattc tcagaaggaa gcgaagaaac atcttcaacg
     2581 ggatttttag caatcacccc ctctttaaca gcccgatcca atacaatttt taggtgccct
     2641 cgaatccgat tgatataaga ttttgataac gtttcgccat taactttttt ccgtgaatta
     2701 cgtgactctg tctggcttag ttcaaataac caatcctcaa tatcgtgact tgtaatatct
     2761 cgaacacgaa aatgatagaa aaactgcata cgcttttcga aaatgaattg cgcattctca
     2821 tatgtttgac ttttgacctg tctctcgtac cattttacaa acctatcctt gtaaaactct
     2881 tcgaaagtca tttgattggt agaacacagt tgattaggaa ttgtcttgtt aaaatcatgt
     2941 gtttccatgt acttgtcata agcaattttg gcatcttttt gtgaagcata acctcgttta
     3001 gtcttctgaa cctttcttcc attttcatct atacctagat acgctcggaa gaaccatttt
     3061 tttgtctttt tatcttggta aatattttta atttttggca tcgttccatt cctccctgta
     3121 cttgagaaaa gctactttaa tagtaccgcg cttgtactgt ttattccgca acttgaatgt
     3181 gaaaaagatg ttcaattact cgggatggca ccacaccaac ttggcgatta ttataaaaat
     3241 caataccttc aatccttgca agatatgcct tgctctcttt aatcattcgt gcagcttgac
     3301 taggtttaaa gcccatctct atcaaaattg cacgatttac catgaaatca cattttgttt
     3361 ccattctctc acctcctgat tctttactcc ttttaaaata atcctcttta taatctattg
     3421 tttctcattc gatggttgtg ttatactcgt cttgtcgagg cggagtacgt tgcacaactt
     3481 gttgtgtaag cagtactttg cttttttagt agcttgtttt ctcatttgat atactttgta
     3541 attttcgttc ataatactca acctcctcct gataatactc cgggtctgcc ttcatatttt
     3601 ggagaagttt gatctgtctt ggattcattt ctccttgttc aataatcaaa ggcaacaagg
     3661 aagaatgaaa cgtttgatcg acctgtgcaa taaaaactaa tgatggtgaa acaccatgaa
     3721 ttaaccaact catagttttc tcaaaacttt gaggaattgt atctaaagaa atggtgattg
     3781 cgccataatt gctaataaag gctgcccatt tggcatcaac ttttgcacct agatcatttg
     3841 gatcacggtc ataaaaacaa attgctccat taagtaactc aaagaacagt ttggttaaat
     3901 catgggtttt tgaaataatc tttgccaagc tttgcgcttt ctcttttcga taccgtagtt
     3961 caaagcgatt ctttactttt gcctcttcta aaggaattcc tcgtcgcttc cgttgttcat
     4021 ggtccttctg ataaaagcaa aaacgacact gactcttctt tgagcctaaa tacaaggtct
     4081 caccatttga ttcattgttt atgatatccg taccgccatg agattcactc acttgaaaag
     4141 tggtccacac atggcctagt attaccttat gcttaagttc tggaatgctg agactcccca
     4201 caaaatcatc taaagtaaaa tcaatccgtg taaagttccc ctgataatct aataccttac
     4261 gaaaaaactg ttgccaattt gtttttcgtg tttttagcca tcttgctaag tgtttacagc
     4321 cttgacctga aaactccatc acagttcctt taaccgcatc gtcaatggaa taccgaatcg
     4381 tgattacttg attccacacg tattggccaa tatagccctt tggcgcataa tccagttcct
     4441 caaaacgttc aatgggcatt tcaataatct cttcaattag ctttaaatgt tgattcgaga
     4501 aaaagtgaat ggttagataa tcaatcaaaa cacggttaac tggaacgacc tcttcttgca
     4561 aactatccaa cacaagattc aatttttctt cacttaattg tctcgttcct ttttctactt
     4621 tcgataataa ctatctcgaa atccccactt tttctgctaa atatcgttgt gtccaccccc
     4681 tttcttctcg agaaactcga atggcttcac cgagatcatg ttgattaact tggttcatcg
     4741 acaaacctcc ttctgctatt tacttgtcaa agaacctatt gaacttattt taagtacatt
     4801 tttattgtac gcctgtattt attttaagtc aatatgtttt gacttttttt aagtacacac
     4861 gtatactaaa tttaagaact tatcctgagg aggagttttc atggagatag acaagcaagc
     4921 agtcggcaat cgaattcggc agattcgcca agaattaaag ttgagtatgg agaaatttgg
     4981 caaactaatt ggtgatttac cgcgaagtac agtcaataac tgggaacgtg ggatcaacct
     5041 tccaaaaacc gaaacccttc accaaatcgc agagataggt catgtcacca atgaatatct
     5101 tttgtatggt gatcaagaga atcaatacat tttagaaatg ctccaaaaaa aggcaggtaa
     5161 gcttgatcct aagattgaag gtctcatagt agacgaaatc aaacacgctg accttgttag
     5221 tgaaaaagaa atggatcgaa tgattgaatt ttttattaca aatctcattc caccaactga
     5281 acaagaccag tttactttcc aatgtatcga tgaaaaaaag cagctttatc ttggttcaac
     5341 taattttggt aaggaagccc agctctattt gcaacatgat aatcaaaatc atattctgca
     5401 catgatgccc tttaccttct ccaactttag tgtggatcgc atactcgtct atttagcaaa
     5461 tcaaaaatcc tattcctatt ttgggaaaca tttacccaag gaactaggtg agaaagccat
     5521 ccttctatac tcaattaatc aattgaccag cgatgtgcga attgccccgt tggtctattc
     5581 agaagaaacc gcttcttatc agtacacaga ggacaatcaa tatctactag aacagcaata
     5641 tctttatcta ccattcgtga tggaagtcga gaaagaacgc ctgttaaatg cagcgtatcc
     5701 atcatcaaaa tgaggtgaca aaattgataa gcacgtgaca aagaaaatac tgctaggaca
     5761 ctccccaatg ggtagttgtc acagtttcct gttctttgtt agccccctgt tagaaccggg
     5821 ggctttattc ctgcagtccg gctacgcctt cctgcaatag acaaaactcc agatcgggta
     5881 attcgcttgt ggcgaaaccc gacctggagt ttgtctctgt attttgaacc ctaaaaatta
     5941 atcaaacaga ctgatattct cataatcagc gcgaaataag ccattaagta cagttttcct
     6001 acctattttt gataacccgc tggttagtta atattccttt tccgagagag ataaatgatt
     6061 ggttaaatct atatacatct caacctttaa aaatgttttc gaaatacttc atcagtacat
     6121 gtcatacaaa ttaccagtgc attctcaaat tctagttctt taattgattt cagttgagga
     6181 gcagttactt ttggcggaaa tttccatttt tgtccaaacc tcttcccccg atgaaatttc
     6241 tttgttgcaa ttattgcaat gaaaaatttt tgtttcaaaa atatacataa ttacttcctc
     6301 caataaaaac tatctacctc tcaaattatt tttctctaat aagaaaaaca gaaaaggtaa
     6361 taagatacaa cagtgatgat tcctaataaa
//
LOCUS       DANKBF010000107         6364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000107 DANKBF010000000
VERSION     DANKBF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..2027
                     /locus_tag="PWY89_002729"
     CDS             <1..2027
                     /locus_tag="PWY89_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2067708.1"
                     /translation="SVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQLIKL
                     SFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETASEMY
                     FGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQTEYD
                     QAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLDLDA
                     QKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLAVNT
                     SRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTITLRQ
                     ALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYGASS
                     LKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDILKDT
                     ITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSISVWT
                     GYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQYTA
                     RSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSEPAS
                     STTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            2324..2857
                     /locus_tag="PWY89_002730"
     CDS             2324..2857
                     /locus_tag="PWY89_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067709.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(2983..3660)
                     /gene="nth"
                     /locus_tag="PWY89_002731"
     CDS             complement(2983..3660)
                     /gene="nth"
                     /locus_tag="PWY89_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL2067710.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3667..4365)
                     /locus_tag="PWY89_002732"
     CDS             complement(3667..4365)
                     /locus_tag="PWY89_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2067711.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            4633..5817
                     /locus_tag="PWY89_002733"
     CDS             4633..5817
                     /locus_tag="PWY89_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL2067712.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(5823..6266)
                     /locus_tag="PWY89_002734"
     CDS             complement(5823..6266)
                     /locus_tag="PWY89_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2067713.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1826 a         1290 c         1099 g         2149 t
ORIGIN      
        1 tgtcggtaga agatcgccgt ttttataaac acatcggggt tgaccctatt cggatcatcg
       61 gttctgccct ctctaacttt acctctggcg gactgcaagg cggaagtact ttgacacagc
      121 aattgatcaa actttccttc ttctccacca gtgccgagga tcagactctg aaacgcaagg
      181 cccaagaagc gtggatggct gtaagattgg aacaaaaaaa atcgaaacaa gagatcttga
      241 cctactacgt aaataaagtg tatatgtcaa acggcttgta cgggatggag acagcttctg
      301 agatgtactt tggtaagaaa ttatcagaac tttctttacc gcaaacagca ttgcttgctg
      361 ggatgcctca agccccttcc gcctatgatc cttatgttta tccagatcag gcgaagaaaa
      421 gacgagatac tgttttgtat acgatgcttc aaaacgaaaa aatctctcaa acagaatatg
      481 atcaagctgt taatgtaccg gtaacggatg gtctgcaaga actaactcaa tccgatgaca
      541 atactaagat cgtcgataat tatgtcaaag aagtcatcaa tgaagtacaa gaaaaaacag
      601 ataagaatgt ctacacagac ggtttagaga tttatacgaa tttggatctg gacgcccaaa
      661 agaaactata tgatatcgtc aacacggatc agtatgtctc ttacccagac gacgagatgc
      721 aagttgcctc cacccttatc gacaccaata ccgggaaagt aaaagcccag attggcggac
      781 gtcacatcgc agaagacgta actttaggca acaacttagc agtgaatact tcccgtgatt
      841 ttggttctac gatgaaacct gtcacagatt acggaccagc ttttgaatat ttgaaatatt
      901 ctactggaaa aactattact gacgcaccgt ataactatga agggacttcg actcctgtag
      961 gaaactggga taatcaatat atgggcacca ttactttacg ccaagcactt tacttatccc
     1021 gtaatgttcc agcagtcaaa ttattcaatg aagtcggttc agataaagtg gcttctttct
     1081 taaaaaatct tgggatcgaa tacagtacga tccatcaatc gaatgcgatc tctagtaata
     1141 cggaagaaca agatgggaca aaatacggag cttcttcctt aaaaatggca gcagcctatg
     1201 cggctttcgc caatggaggg acttattaca aacctcaata tgttaataaa atcgtcttcc
     1261 aagacggaac agaagaaact tatgagccag atggaaaaac agccatgtct ccagaaacag
     1321 cttatatgat cacagacatt ttaaaagata cgatcactga aggaactggt acgaatgcgc
     1381 aaatcgctgg gttatatcaa gccggtaaga ccggaacatc caattacaca gatgacgagt
     1441 atgcaaaact tggtatttct tctggggtgt atccagatat cctatttgcg ggctatacgc
     1501 caaattattc gatttccgtg tggacaggtt acaataaaaa aatgacgcct gtcacttcag
     1561 aatcgtcaca cgtagcctct gatgtttatc gcgagcttat gcagtatgtt tcagctaacg
     1621 tgacaaatac agactgggag atgccaagcg gattaattcg tgtgggcggt gaactgtact
     1681 acaaagatca atatacagct agatcgaatg caattacacc atcaacaact attccatctt
     1741 ccagctatgt ccagactcct ggaagctcaa caacagagac aacgactcaa agctctagct
     1801 ctactagtca aagcgaatca actgctgagt cctcaaaaga aagcacgaca gcagaaacaa
     1861 gcgagccagc gtcttcaaca acggttcctt cttcttcatc cgaagaaagc tcgacaccgt
     1921 cttcatctgc tcctcctgct tcttccagtg agccagcaac tagcggagcg gatgctgcaa
     1981 atgaccatac accatccagc agcacctcag cttctggaaa cagatagtaa aaaagaaacc
     2041 acgtctacaa gcgtggtttc ttttttgtgt ctatgtaaaa aacagctaca aataaaaatc
     2101 atgtaccaag ctgtattaca tgatttttta taagactatc cagcatatac atgtgtgatc
     2161 taacatttag cttaaaaatt agaaaatcca tttttctttt atcattacat atactttatc
     2221 catttacttg tttataatta aatcggctaa atacacacta gtcttttatt tagtgccact
     2281 tcttaataat cccattgtta ttatcaaata aaaaatacaa tatatggttt tgaataattt
     2341 tttgaaagaa ggcttcattt tgagttatct gatagtatgc ttaattatca taatttgtct
     2401 gcaccatctt tttttatcga aaacaatacc tcgttattcc aagaataaaa aagctgaaaa
     2461 attttgttta ttattaaata aatttacttt aattgctcct atccttgcat ttattatttt
     2521 ttctgtactt ttaagcacta ctcttaaagg aaaatttatg gaaagaagct ctcatgcaat
     2581 gattctcact tttttatggt tgttgttcac cagaatctat atttttctga tgagcctgaa
     2641 accgcccaaa tccattagtt tatgtttagt tataaatggt atttttttat tatcacttat
     2701 catttttatt acacctttag atagatacgt cacttattta tataatcctt tggaatactg
     2761 gacttatttt ataggtatat tggaaggcat tattttctac atcgggtatt ttcccaataa
     2821 aaataacaac ttttattttt atcgcaataa attgtaataa gttaccatcc tagctactga
     2881 gtatgtacat ttactttttc atgcaaaaaa gataaacgaa acagcttttc tccataataa
     2941 caagataatt catacattta tttgcaatta aagaaaccac gtctacaggc gtggtttctt
     3001 ttttagcgcc ttttctttta gacgcatccg atttttccca tcttgacata ttgatagcaa
     3061 aggacatacc tcgcacttgg gatttcttgc cgtgcaatga tatctaccaa aaaaaatcag
     3121 tgtatgatgt gtctttaccc ataattcttg tggtactttc cgcatcagtg tttcttctac
     3181 ttccatgacc gtagcatcta gtttgcagat cctcaggcgt tttgagacac gttccacgtg
     3241 ggtatctact gcgattgctg gtatcccgaa tgcatccccc aatacgacat ttgctgtctt
     3301 tctgcctaca ccaggaagag acatcaattc ttctcttgac gttggcactt gcccatcgaa
     3361 acgttcaatc agctgctgtg cacacgcttt gatgtttttt gctttatttc gatacaaacc
     3421 aatcgttttt attttaagaa tgatttcatc gattgaagct tctgctaacg catcgggtgt
     3481 cggaaaagag gcaaacaaat ctggcgttgc tttattcact gatacatctg tcgcctgggc
     3541 acttaagatc actgcaatca ataactcaaa tggattattg tgcttgagtt ctccatgtgc
     3601 ttcgggaaac attccataca tcgtttctag agcttccatt gttttttgtt tgcttaacat
     3661 attccgctac tcctccaacc aatttttcag tgagactttc ggcaattctt cttgttgagc
     3721 tgactgttct acttctcttt gaagaagctg ctgttttctt cgtttttggt cttcttccac
     3781 ttgttgtttt gtacgtagat tttttctttc ccacgatagc aaaatgcggt caacgtaatt
     3841 aaaactataa gcctgattta atacggcttc ccgcagtgct agcttcaaaa tttccggctg
     3901 ataatggtct tcttctagcc attgtccgat ccgctggaac tcgatcgaag aaagcgggcg
     3961 tccaaattct tgttcaaata attgatacac tgactggatt tctttttctt gtgttttttc
     4021 ttcgtatttt ttttcttgtg tttttaaata ctcacctaat agatcataga tggggtaaag
     4081 attatatcga tctgcttttt taccgttgac aatcgtcgtt tctatcttga taaatccatt
     4141 tgttacgaga cgatccagta tttgaaaaat ctgatcttgt ttgattccca tatccatagc
     4201 aatcatctgc aaatcaggga aagtatctcc ttcaagctgt gccatatgca actgtagaac
     4261 aaataaaaat tcttcagatt gtagaccgat cctatgataa tttttaagta aaagattaga
     4321 tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc aacatgattt ctcctccgct
     4381 caatcacagt atacgtaaaa aaaacatggg aagcaagaac aggtttcttt tctatttact
     4441 tcactgatcg gtcagattta caatgtcgtc tacttgattt ttgtttgttc atgtttttga
     4501 tgcgtatctt cacttttcgg aataaataaa ttaagtagat ttcgctgttc cttagaatgt
     4561 tgacaaggaa acaaaattac tatacactat ttttgtttcc aagttaacat tattgcataa
     4621 aggagttttt acatgtacag tttcacaaat aaacattcta aagtttttgg atacgtctct
     4681 gtcttattca ctggattagg atttactatc gtcagtcctg tacttccgtt tttaacgctg
     4741 ccttattctc actccataca tcagcaagct ttctatatca ctttgctcat gtctgtttat
     4801 gctctagctg ccttcttatc tgcccccatt ctcggtagct taagtgatca ttttgggcgt
     4861 catccgatcc ttattatcag tttattagga tcaagtatcg gatatttgct ttttggttta
     4921 ggaaattcga tatggatgtt gttccttggt cgtatcattg aaggattgac ggcaggagaa
     4981 atctccacct tatatgctta ctttgccgac atcacggaac ctaatgaaag aaccaaagtt
     5041 tttggatgga tgggtgcttt agtcggtata ggaacaacgc tgggtcctat tatagggggt
     5101 ctattagctg agttggggaa ttctgttcct atatttattg gagcactctt tactttctta
     5161 aatgccgttt atggctacac atttatgcta gaaagccttc caataaaaaa acgttcagta
     5221 gatttaagtt tctcccatgt aagacctttt caccaattac aacaattatt caagatttca
     5281 tccgttattc ctttattgac agcaggtttt gctgtttggt tggcagctgg ctctcttcaa
     5341 tctatctttt ctcaattttc gattgacgcc tttcagtgga aggctgggtt ggttggttta
     5401 agcttctctt tgattggaat cttggatact gtctcgcaat tgttcattat gcctcgattg
     5461 ctcaaaaaat tttcagaaca gcagatcaca agaataggga tgttcagtga aatcctcgcc
     5521 tatctattca tcacattatc tggaattctt ctactgccga ttttgttttt atttgggatt
     5581 atctgttatg gtttcggtga ttctattttt actcctgtat tcaatggcca actatccaat
     5641 tctgtctcag ataaccagca aggtcttgta atgggcggta cccaaagtat acaatctcta
     5701 tcgcgcgtca tcggcccgct aatcgcagga cagctttatg ctgcagcacc atttcttccg
     5761 acggtattcg gatttttgct tatgattttt gcctattatc actacaataa aaaatagtag
     5821 cttcatttgc tacttcgtat aaattctctg tatcgaccga taaaacgaaa tactgtgtca
     5881 aattcttctt ttgacatatt ttcaaataca gtagcatctc gctgtaccca tttttgatgg
     5941 agttgctcgt gtctattcct tattttttgt ccgattggcg tcaattgaaa atagatttct
     6001 tttttattct cttcgctttg ataagtgttt atgatccctc tagctatcag tttttttgtt
     6061 agcttactga tggctcctct agtcatgtaa ctagcattcg ctaattttgt cacgttagga
     6121 tcagggattt tcccgatatg ttcaatcact tctagttcat ttagagtaaa tcctgcaaat
     6181 tctttttcca tttctggttt attacgatga ataatctcat tagctaaatc cagatattct
     6241 tctattagca gttgtctttt atccatttct acaccttctt tttctttcat tatattgttt
     6301 ttttgtttcc tattcaacaa agataaaaat attttgtagg aaaacccaat ctgaaaccaa
     6361 gaaa
//
LOCUS       DANKBF010000108         6354 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000108 DANKBF010000000
VERSION     DANKBF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..333)
                     /locus_tag="PWY89_002735"
     CDS             complement(<1..333)
                     /locus_tag="PWY89_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2067714.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELP"
     gene            complement(323..970)
                     /gene="recU"
                     /locus_tag="PWY89_002736"
     CDS             complement(323..970)
                     /gene="recU"
                     /locus_tag="PWY89_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL2067715.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            1034..1573
                     /locus_tag="PWY89_002737"
     CDS             1034..1573
                     /locus_tag="PWY89_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL2067716.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            1663..2082
                     /gene="gpsB"
                     /locus_tag="PWY89_002738"
     CDS             1663..2082
                     /gene="gpsB"
                     /locus_tag="PWY89_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL2067717.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            2104..2471
                     /gene="rnpB"
                     /locus_tag="PWY89_002739"
     ncRNA           2104..2471
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWY89_002739"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            2680..3846
                     /locus_tag="PWY89_002740"
     CDS             2680..3846
                     /locus_tag="PWY89_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL2067718.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            3892..5388
                     /locus_tag="PWY89_002741"
     CDS             3892..5388
                     /locus_tag="PWY89_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL2067719.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            5469..6152
                     /locus_tag="PWY89_002742"
     CDS             5469..6152
                     /locus_tag="PWY89_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2067720.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2120 a         1126 c         1337 g         1771 t
ORIGIN      
        1 cggtaattca ttcgcagaaa ttttttcacg cttttctgcg cctaaatctt cgaatagttc
       61 gccatcttgt gtatatagtt tggaagatac ggtagcgtct aattttttat ccgtcaattc
      121 gggcgcatct tttgcgtaat accagaaaag tcctacacct gctaaaaaga ggatacagaa
      181 aaatgagagt attcctaaaa ggatcttgat aaataatcct tttttatgtg ttccagagga
      241 ctttccagaa cctttgccag aattcttctt cacactcttt ttaggagtag gctgtttctg
      301 tcgtctagag cttctagttt gttcatttgc cattcttgct ttctccttca atagataatg
      361 ttgataaata gaagtcaacc gcgtccaaat aaggaatccg cggagaaata ccagttttga
      421 tctctattcc tagttcttcg atcaaagata aaggtagtga tttgcgtcct ttttcctttt
      481 gggcgtccca gtaagcaatc aaatgaacac tattcaaata aaaacagcgc ttcaaagacg
      541 aaaaccataa caagacaaag cagaccccat tttgttcgag gcatgctttc atatgttcga
      601 tttgatgggc atggaagttt ttaaagggaa aagatgtttt gttcttggtt tcttttgctt
      661 caaagtccaa gtaaaagcct cgatagacgc cattatagtc tgtagttgag gcttgggtaa
      721 agtaggcttc cttgatcaca gcagcacttc tcttaggata gtctactttc acgatttgga
      781 taggggtagg ctttttatgg ataaccgcca acccgcggga aagataatat ttattacttt
      841 cattgattgc ttcttcaaag cgcatccccc gattgccgaa ttcagtgatc tgatttcttt
      901 tttcttgaat tttttcgacg ggagaggcat tcttataata aggttggcca tttggatagc
      961 gcaataccat ctttaatcac actcctaggc cacattatag caaaaaagcg gatagaaaga
     1021 aaaggaatct gagatggata agtcaaaagt aatctatgtg tctgggtatc gaagtttcga
     1081 actcggtgtt tttaaagaaa atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa
     1141 agaactccat cagttagcag aagaagggct ggaatgggta cttgtcagtg ggaatttggg
     1201 cgtcgaacta tgggctgtgc aaatagtagc ggaattgaaa gaagaatatc cggaactatc
     1261 tcttggtatt ttgtatcctt tttctgattt tggtaataac tggaatgaag ggaaccaaga
     1321 aaaaaaacaa ttagcagaac aattagcaga ttatgtcgaa gctgttagcc atcaacccta
     1381 ccaatcacct agtcagctaa agaatcacac tcgttttata cttgagcata cagtgggctg
     1441 tctgctgatc tatgatgaag aattccctgg aaaaacgaag tatttttttg aagatgcaca
     1501 gaattaccaa gcagatcatc cctacgaaat tcggctgatc agtatggacg atcttcaaaa
     1561 ttttagtgaa taatcgatat tgtgtttgat ttatcaactt ttttcctata caatgaaaaa
     1621 ggtagaaatg ttattacgta aatgaaaact agaggtgtaa atatggcgaa tttggtttat
     1681 agtcctaaag acattttaca acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt
     1741 gaagttgatg aattcttaga caatatcatc aaagattatg aaacttacag caaagaactc
     1801 cttgctttac aagaagaaaa cgatcgattg agtgcaaaag tagctcaatt atctaaaact
     1861 caaggagcag ctcaaacacg cgtgcaacaa acagaggcac caaagagcgc agcagtgacc
     1921 aattttgata tcttaaaacg cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt
     1981 gatcaacaag caagtgcagt caaaccagca caacctaatc caaataacta cacaaatgca
     2041 gacacaagct tagatgataa tgaaaaaaca cgtcaatttt aatttttgat taatgaattt
     2101 gcggttttcg ggtaatcgcg gtctgacttg atcaggctga ggaaagtcca tgctcgcaca
     2161 ggctgtgatg cctgtagtgt tcgtgcttag cgaaaccata agctaaggta ctctttggag
     2221 taacggcagg aaaacaagct aaggcttcgg ctatgcttaa gtatccttga aagtgccaca
     2281 gtgacgaagc ggctggggaa acccatccgg tggaacgcgg taaacccctc gagcgagcaa
     2341 cccaaacaat ggtaggggcg ctttttgtct ggaagaagaa caggacaaaa aggcagtata
     2401 actgcagata gatggttacc gctttgtttt ttctccctgg aaaagcaaag tacagaacat
     2461 ggcttataga aaaccgcaaa catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa
     2521 gaaacaagaa aaacatttga aagctatttt tcaatacttc agatgaatcg ttcttattac
     2581 aaaacaaact tttgtatcac cggttattcg gaatttgagt aaaagtgtgg ggtaggactg
     2641 atgtcccacc tccttttttt ataaataagg agattgtgta tggaaaaaaa caggaagttc
     2701 aacctcgtcg caactgcggc aagcgggcta gaagcactgg ttggtaaaga attaagagat
     2761 cttggaatcg aatgccaagt tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg
     2821 atcgctacag ctaatttatg gttacgtaca gcagatcgag taaaaatcgt ggttggggaa
     2881 ttcgatgcgt atacgtttga tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat
     2941 tttcttccac tagatgcagc ttttcctgta gctggaaaat cgatcaagtc gaaactatat
     3001 agcacacctg attgccaagc gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat
     3061 tatcaccgtc cagcttctgt accactgacg gaaactggcg cacattttca gttggaagta
     3121 gctttgctga aagaccatgt gatggtaact ctggatacga caggaccaag tttattcaaa
     3181 cgtggttatc gcttagaaaa aggtggagca ccattgaaag aaaacatggc agcagcactt
     3241 attatgttga caaattggcg aaaagaccgt ccattttatg atcctgtatg tggttcaggt
     3301 acgctttgta ttgaagcagc cttaataggc cataatatcg caccaggatt caatcgtgag
     3361 tttgcttgcg aatcttggga ttggttcagt caagaaatca tggaaaatgt tcgatcagca
     3421 gccgaagaaa aagcagatta tgatattgaa ttagatatta ctggttcaga tatcaatgga
     3481 cggatgattg aaattgccaa agccaatgca gaagaaatcg gattaggctc ttcgattacc
     3541 tttaagcaac aagcagtcaa agacttcaaa acggataaag aatacggggt catcgtggct
     3601 aatccgcctt acggagaacg tctaggagaa gaagaaactg ttcggaaatt atataaagaa
     3661 atgggcgatg tattccgtcc attaaaaaca tggagtaaat atatcttgac tagcgactta
     3721 gcgtttgagg aattttacgg acaaaaagca accaaaaaaa gaaaattgta caatggtgca
     3781 ttgcgtacgg atctattcca gtattgggga actcgtccgc ctagaaaaaa agagaaagac
     3841 gaataatctt actaattgat tcattcaatt ggatgtttgg aggaataggg aatgaaagaa
     3901 aaagagtttt tacaagaagt aaaagaaatc aatcttctac aacaagcttt aggaatcctc
     3961 gattgggata cacaaacagg tatgcctgaa aaagctagtg cataccgaag tgaagtagac
     4021 agctatcttt acagcattta tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt
     4081 cgctattttt ctgaacatcc ggaagaattg tcggaagttg gaactgccgt ttttgccaaa
     4141 gtaaaagaag agtatgattt ggaacatcgc gtaccggaag atcttatgag aaaatataca
     4201 gctgccgttt cttcagcaca cggacaatgg caaaaagcta gagaaacaca acaattttct
     4261 gattttcaac acgcattgaa tcaaaatatt gaattaacaa aacagttgat accttactgg
     4321 agaaaagatg aaaagacgaa ttatgatgtt ttgctgaatc aatacgaacc cgggatgact
     4381 gtcgaaatcc ttgataaggt atttgatcag cttaaagaag gaattctttc gatccgccgt
     4441 acgctccaag aaaaagggac tgaacctgaa acagactttt taaatcggag aatgacgaaa
     4501 aaacaacaga agagatttgt agtgaaagta atcgaacaac ttggttatga ttttagtcgt
     4561 ggtcgtttag atgacactgt ccacccattt atgactggaa ttaatccgaa tgatgcacga
     4621 atcacgacac gatggaacga aacagatttc aaaatggctg tttttggagt gatccacgag
     4681 gctggtcatg gtatgtatga acaagacatc gatgataaat atgcgtatac accggtttat
     4741 gaaggtacat caatgggtat ccatgaatca caatcgttgt ttaacgagat cattattgga
     4801 agtagtcgca gcttctggaa aaagcaatat cctttctttc aagaatgtgc agaaggaaca
     4861 ttcgatgata ttccttttga caaattttat cgttcattga accatacaaa atcaagtttg
     4921 gttcgtatcg aggcagacag tttgacttac cctctccata tcatcattcg ttatgaaatc
     4981 gaaaaaatga ttttcaatga aggggtagat gttgctgaac tacctgaaat atggaatcaa
     5041 aaatatgaag agtatctggg aatacgacca acaaatgatg tggaaggcat cctacaagac
     5101 gttcattggt caggtgcaag ctttggttat tttccctctt atgcattagg gtacatgtat
     5161 gcggcacaat tacttcattc tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca
     5221 gacgattatt ctccaatcaa aaactggatg tcagagcata tccatcaata tggtgcatct
     5281 agaaaaccaa atcaattaat catggatgca acacatgaac cgcttaatcc gcaatacctg
     5341 atcgactata tgaaagacct gtattttaat gtatatcagg taaaataaaa tacaatttac
     5401 aaagatagct gaaattcatc taatctatag atgttttcag ctatttactt aagaaagaag
     5461 tggaacgaat ggataaatta caagtgatcg ctgattatag tatggaccaa ttagcgcatg
     5521 atcaaacagg acatgggagc gatcatacca aaagagttgt caagttagct gaaaaaattt
     5581 tggagacgga accgcaggca gaccgctttg tgacgcttgc agcagcttac ctacatgata
     5641 cgattgatga taaagtcgtt aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc
     5701 ttcgctcttt gccaatagta gaggaacaga tctcgatgat tttttccatt atcgaaaata
     5761 tgtccttttc taaaaatctc tctgaagcgg ccgaactatc tctggaaggt aagatcgttc
     5821 aagacgctga tcgtatcgaa gcattaggag caatcggcat tttgcgtact gcttatttcg
     5881 gcggtggaca tgggcacccg atttttgatc ctgaactgta tccgcaaaca ttcaaggaca
     5941 aagaaaatta ccgaaaaggc acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc
     6001 tgtctgataa aatgaatact gattacggat atgaagaagc caaacgaaga gaaaccttca
     6061 tgcgtgaatt cttagaagaa ttttttctgg aatggtctat tgacgatgaa aattttactc
     6121 cagacaattg gatcaaaaat aacaaaaaat gatggaggtt gtgacagaag tggtcagctc
     6181 caagatattt cggatcattt tccgtaaaag ttacttctgg aacactgttt accaagaggt
     6241 cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg
     6301 tttgatgaat attgtaactt gtgattcgga attggcagga gaacaccgtt tatt
//
LOCUS       DANKBF010000109         6268 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000109 DANKBF010000000
VERSION     DANKBF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..561
                     /locus_tag="PWY89_002743"
     CDS             <1..561
                     /locus_tag="PWY89_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL2067721.1"
                     /translation="PVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKI
                     DYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNS
                     KYTKLLIKNFLISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKE
                     DYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            596..1363
                     /locus_tag="PWY89_002744"
     CDS             596..1363
                     /locus_tag="PWY89_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2067722.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1555..1953
                     /locus_tag="PWY89_002745"
     CDS             1555..1953
                     /locus_tag="PWY89_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL2067723.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2260..2634)
                     /locus_tag="PWY89_002746"
     CDS             complement(2260..2634)
                     /locus_tag="PWY89_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067724.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2656..3033)
                     /locus_tag="PWY89_002747"
     CDS             complement(2656..3033)
                     /locus_tag="PWY89_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067725.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(3023..3418)
                     /locus_tag="PWY89_002748"
     CDS             complement(3023..3418)
                     /locus_tag="PWY89_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL2067726.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3485..3670)
                     /locus_tag="PWY89_002749"
     CDS             complement(3485..3670)
                     /locus_tag="PWY89_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL2067727.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3752..4006)
                     /locus_tag="PWY89_002750"
     CDS             complement(3752..4006)
                     /locus_tag="PWY89_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067728.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(4028..4273)
                     /locus_tag="PWY89_002751"
     CDS             complement(4028..4273)
                     /locus_tag="PWY89_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067729.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(4280..4564)
                     /locus_tag="PWY89_002752"
     CDS             complement(4280..4564)
                     /locus_tag="PWY89_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067730.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4667..4849)
                     /locus_tag="PWY89_002753"
     CDS             complement(4667..4849)
                     /locus_tag="PWY89_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2067731.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(5055..5594)
                     /locus_tag="PWY89_002754"
     CDS             complement(5055..5594)
                     /locus_tag="PWY89_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067732.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKIYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(5707..5973)
                     /locus_tag="PWY89_002755"
     CDS             complement(5707..5973)
                     /locus_tag="PWY89_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2067733.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5975..6196)
                     /locus_tag="PWY89_002756"
     CDS             complement(5975..6196)
                     /locus_tag="PWY89_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDL2067734.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
BASE COUNT         2015 a         1034 c         1067 g         2152 t
ORIGIN      
        1 cctgttttct ccattgcaga tttacgaaag aaatttagta ttgagaaaaa atataaagct
       61 tttcgagact ttgaaagacg tgtaattaaa gaaccgattg cccaactgaa taaatacact
      121 gattttaaaa ttgactatga gcttatcaag aagggtcata cttatacaaa cataaagttc
      181 ttgcttacac gtaccatgtc agttccggta ccttataagc aagaacaaag agaccctgct
      241 tatttaaaag gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg
      301 aatagcaaat acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc
      361 gatcttaaac ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc
      421 aaagatagag gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag
      481 gattacacaa taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct
      541 tatgtgaacc tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa
      601 aaaagatgaa ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga
      661 tttaaagtcg tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa
      721 gaaaacaggc gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca
      781 aaatcttgaa acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga
      841 aatttcgaat ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat
      901 tcattgggat ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt
      961 aattgtctca atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca
     1021 aaaaaaatat agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat
     1081 cgccaaaaat aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat
     1141 agttaaaatt cgtgagatta cgaaaacaga aaatgagtat aaaacaatat cgaattttga
     1201 tagacgtgtt ttaagggaat cagttgagga aattaatgaa aaatcaatgc tgaatattga
     1261 ttttgctaaa gttagaaaag gcagaaatta caattttgct gtccagttta atatttctag
     1321 tgagtttgat gaaaaaactt atagagaaaa aaaattaggt taatgcaaaa tatatcttac
     1381 tcgtttgaag atgcttcaga cgtatgctaa gatatatagt gccttgggag caaggtatta
     1441 atctccttta gaggttaatt agcttgtgat cgcctatcaa tttttttggt aggcgattct
     1501 tttttttgaa acaaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa
     1561 tacttttttt gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat
     1621 tttccagact tatatacaca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa
     1681 gtattggaag gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg
     1741 cctgttcaag agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta
     1801 aaagctaata cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg
     1861 actgtggaac actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct
     1921 ttgaatgatt tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa
     1981 aaacagcttg ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa
     2041 aagctctcag aagccttctg agagctttta aattgctatg tattgtactg catctgctca
     2101 aattgatttt cttttcatgc ttaccatgta tctaattttc catcaagtaa tttttagata
     2161 tgtattgagt aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat
     2221 gtgttaggat aaaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt
     2281 tttatcggta ggcttgttta acgtgatctt gaaggatacc cttttgatgt ttctctcgat
     2341 ccctgcatcg ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat
     2401 caaaatggtg tagataagca aaaaactccc taaaactaag tgttgattat aaaaatctga
     2461 cttataaaaa agaactgaga atgttagtgt tgattcccaa aaaagaaaca aaattttgta
     2521 tttccaaaga tagtggatca agcgctgtgc tttacccatc tcaaataatt tataataacc
     2581 tgctaattga tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt tcatctttct
     2641 cttctcctgt ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc
     2701 ggtttgaaat tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga
     2761 ttcacttaat gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt
     2821 atagcagaat tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa
     2881 gttattatcc tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc
     2941 agttgtatct tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc
     3001 tttcataggt tcggtctcct ttttactttt caagcaactc ttcttccagt gctttttgaa
     3061 gtagttgaga gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac
     3121 taacattttt tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt
     3181 ggatgattac taccacttgg ttggctttca aagttaattc ttccactgct gatggtttag
     3241 gaaattctgc atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat
     3301 atgcttcttc aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt
     3361 tataagcttc tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt
     3421 cctcgctcct tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag
     3481 gctgttaaag ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat
     3541 tgtggatcgg aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc
     3601 cagtttgtcc agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg
     3661 tcttaggcat agctaccctc cctttacaaa atctattata cacattgttg tgtatggaat
     3721 caatagaaaa agaggatgtt ttattgcaaa actaataaaa tgatttattg gttttttcag
     3781 caaattgaat gttattagcg ttgcaaatat tcatgatttc ccaagtatca tcagaagtat
     3841 aggtatctaa gaattcattg acagttacgg ttgattcgta tggttcataa gtcaaagcgt
     3901 tctccaagtc ttttgatgaa ataatcaaga ctttttcaat ttcctcccct tcttcatcta
     3961 tgaaaacata ttctaaagtg atttgttctt catcggtata attcatattg tgttctcctt
     4021 tcttgtacta agaaaaatgg atatttccaa ttgcatccca ctcggcacca ttgtggtata
     4081 ggacgtgacc gagcgttacg cctttgaaat gttcttctaa atacatccaa acgcattcaa
     4141 ggctagtacc tgctttgaga ttgtagaagt ctgcatccaa taccttttca gaagttatcg
     4201 gatcaatgcg agttggtact tcaataaatt cagtccaaat ttctgcaaaa gtcttacctt
     4261 ccaaagtcca cactccctat caattaattc caaaagcaaa tagagaaatc aagcgaccgt
     4321 caatagtcac ttcaacaact gaaaatgccg ttgaaacagt tttaacatct acactttcca
     4381 agttcccgat gaaggaatct ttgtgcttta gaatagcttt ctttagctgt ctgttataca
     4441 cttgccgact gttaaacttt ttgaattctc gatccttgat cagctgttta acgatccctg
     4501 cattaattgt ataactttct gcaatagcaa cgtgagtagg ttgctgtaaa gtagctgtta
     4561 gcatcttgtc actccttcct aaaagttaat tagcttgttt acaaatacat tatacacatc
     4621 ggtgtgtata atgtaaagag aaaaaggtgc taatggatca gctgttctaa acaaaagaaa
     4681 acgataaagt aacaaataaa gtcagcaaag ttaaaaagga tgaaacggct agtccggttg
     4741 ctttgcttgt gtgattttcg gctttcttat agatcaaata ggaaattatc agtgatattc
     4801 cagtaaagaa taatgtacta gctgtcacta aaagaaatag gttaatcata cgatacactc
     4861 cttctgtaat attggtatca gatacgttta aatgcgtttc tgagctgttt tggatcagat
     4921 ggtgatgttt ttagccctac cctaaaaaca aagcccagaa aagaatttaa aagcgttcgt
     4981 acctaatacg tttatattca tatacatata cataaacata aacgtgtata cgtttatgtt
     5041 tatgtattta agttttaggc tacttcctta acatctcctg ctgatagtcg agtgaaccga
     5101 gtgagtgatc catcttgtag tcgaaaaata aagctacgtt tgagtttata ctgatagaca
     5161 gcgacatacg atccatccca gattgagtaa gttttacctg caactaataa gtcatcaagt
     5221 tttggtttat tgctttcttc tttaattcta tctagttgtc gcttaaccat tttttcttga
     5281 tcaccgacaa agaatttcaa ccattttttc ggaattttct taaagtaagc aggactgtca
     5341 tttaagaagt agattggttt gtacatttca tctcgaaaga tgaagctgtc aatatcgtag
     5401 gttccttctg catctttgaa aaggataatt ccatagatct tcgttacttt ggtatctacg
     5461 atttccaaac catttttggc gtaccctttc ttttcttcga tcacttcatt tgctgatgaa
     5521 taatcaccat accatcccat aaaaaatcgc tccttttgaa agttaattag cttgtttaca
     5581 cttatattat acacaccggt gtgtataata tcaagaggat attgttcttt tttaggtaaa
     5641 gtgggatcgt aaacagtgat tttgttgggt taacaaaaaa acagacaagc cttatcagct
     5701 tgtctgttac ttataaactt cacttctgtg accaatagac aatataagaa ttgtcacttc
     5761 atgctcgcta atatcagcta ttatacgata tgcacctacc ctatatcgcc agtagcctga
     5821 tttattagct gttagacctt taccatgctt tcttggatca tcagtgccta caagattttt
     5881 ctcgatccag tttaggataa cctttgcctg aaacttatcc atttttttta gcgctttttg
     5941 agcttcacgt tcatatctta cttggtaagt catcttataa gcctaactct tttagcatat
     6001 cttcgtggga aatactttga tcgttttcct catgctcttt cattagttgc ttgtaggtgg
     6061 caaagtctac taacgtttct gctgtttgca gggaattact acgcataaat tcagaaatgt
     6121 tatctccgtt gaattcagca actttttcaa gaaaatcttt ttcttcttta gatacacgaa
     6181 tacttattac tggcatagtt tatcaactcc ttttcaatta cattgtacta cgattgtatg
     6241 acaaatgcaa tagttatata tttgaata
//
LOCUS       DANKBF010000110         6173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000110 DANKBF010000000
VERSION     DANKBF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            725..811
                     /locus_tag="PWY89_002757"
     CDS             725..811
                     /locus_tag="PWY89_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2067735.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PWY89_002758"
     CDS             complement(932..1621)
                     /locus_tag="PWY89_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067736.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="PWY89_002759"
     CDS             complement(1628..1906)
                     /locus_tag="PWY89_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067737.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="PWY89_002760"
     CDS             complement(1866..2780)
                     /locus_tag="PWY89_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2067738.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PWY89_002761"
     CDS             complement(2762..3142)
                     /locus_tag="PWY89_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2067739.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="PWY89_002762"
     CDS             complement(3592..3879)
                     /locus_tag="PWY89_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2067740.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="PWY89_002763"
     CDS             complement(3899..4123)
                     /locus_tag="PWY89_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL2067741.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="PWY89_002764"
     CDS             complement(4578..4748)
                     /locus_tag="PWY89_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067742.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="PWY89_002765"
     CDS             complement(4763..5296)
                     /locus_tag="PWY89_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2067743.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="PWY89_002766"
     CDS             complement(5317..6159)
                     /locus_tag="PWY89_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2067744.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1634 a         1152 c         1041 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtacatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DANKBF010000111         6116 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000111 DANKBF010000000
VERSION     DANKBF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(108..1559)
                     /locus_tag="PWY89_002767"
     CDS             complement(108..1559)
                     /locus_tag="PWY89_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2067745.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(1880..2644)
                     /locus_tag="PWY89_002768"
     CDS             complement(1880..2644)
                     /locus_tag="PWY89_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2067746.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2672..3370)
                     /locus_tag="PWY89_002769"
     CDS             complement(2672..3370)
                     /locus_tag="PWY89_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL2067747.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3382..4161)
                     /locus_tag="PWY89_002770"
     CDS             complement(3382..4161)
                     /locus_tag="PWY89_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2067748.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4177..5121)
                     /locus_tag="PWY89_002771"
     CDS             complement(4177..5121)
                     /locus_tag="PWY89_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2067749.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5167..5946)
                     /locus_tag="PWY89_002772"
     CDS             complement(5167..5946)
                     /locus_tag="PWY89_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067750.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1675 a         1273 c         1010 g         2158 t
ORIGIN      
        1 aaatttatct gtaatgcgtc atcaaaatct agattccaaa tctttatcaa tttttatcca
       61 ctcattctac cactttagta atattttcta tttataacac tttcgtccta cattgatccc
      121 cccctctgaa gtacagtttt tactgtctta aataaaattt taaaatctaa ccccatactc
      181 caatttttta tatattccgt gtcaagtttt gctacttctt caaaatcagt aatatcactt
      241 ctaccactta cctgccacat tccagtaatt cctggcttaa tagcaattct tgcacgatga
      301 tgataatcat attcttcata ctctgattta aggcatggtc gaaatcctat aaggctcatc
      361 tttcctgtta gaacgttgaa aaattgaggg aattcatcta aagacgtgga tctaataaat
      421 tgaccgattc ctgtcttttt tgttccatct ggaagtattt tatttccgat aattcttgga
      481 tcaaactcca tcttaaacat aagattattg ctcatcttat tttctttcat taattctact
      541 tttctttctt ctgcatccat atacatactt ctaaacttat acatcttgaa cggtttacca
      601 ttctgaccta tacgttcttg tgcaaagaaa attgggcctg gtgaattaat ataaataagt
      661 ggagcaataa agataaaaat aattcccgtt gcaatacatc caacaatacc tgcaagaata
      721 tcaattgttc gttttataaa tgcctgttga gttgtcataa cattgatact tgttgtaagt
      781 actgtatatt ctcctatacg accaatagat tgttttccca aagcactatc tgatactttt
      841 gcaagattca aatgtacagt aaggcccatt tcagagcatc gttcaataaa ttctttcata
      901 cacgaagtac ttttattcaa aataataaag acttcatcta cccatccctg acataagtaa
      961 tccgctgcat ttttggaatc agaaacaact gtcactccct caacatcttc accaatcatg
     1021 cttttatcta tgataatgac accatttatt ttatacattt tatagttatg ctctttcaca
     1081 ttacgaataa ctacttcagc aatactacta gatgtaacta taataagtga tttgttcccc
     1141 tcatttttca ttttatgttt caagtaatgt ttccataaga ggcgagttat atatgtaatt
     1201 acaacgtata aacctcccat caagaaaaga acgactctag aataatcatt tccaccctga
     1261 acactaatga gatacaaact tcctaacagc accaacataa tcgcttgctt tacagaagaa
     1321 gcaaattcta tatagtaccc tctttttaat acacctctat atgcttcaaa catgaaaata
     1381 actaaaagat ctgccagttc aatgaagact gccatgtttt gatacattgg aatcacataa
     1441 ggattccaat ctacctggcg taagacatac gatagataaa aagcaagctg gatacacaat
     1501 aaatctaaaa taataaaatc aatatgttta acccatcccc ttgaactttt cttgtacatt
     1561 cctttttact cccctctttt taataaaata aattgtatta cttgttttct ctcctaaata
     1621 tctttctgaa ggattattga ctcgacaaat tggcttgtgg atattcttat atatagaaca
     1681 tccgtaagcc aacttaaaac tccttaacaa aattttttta ctcatattgt agatcaaaac
     1741 aagtatccaa gaaatcatat tttatttctt ttttctgctt actcaacaac agctaaaaga
     1801 aaaatcatca taaacaaatt caactaagcc aacgatcccc ttgtgaaatc aagttttttc
     1861 ttgatttacg aataaagagc taaaataaac caaacttctt cttttttacc tcttcataga
     1921 ctggataact gatttcatcc ccgttgatca gatcctttgc gacttgtttc atctgtacta
     1981 ctttttcctt accaaagtcc tttgcaatct gctcatagca ctcttttaag taaaaggtgc
     2041 gcttagagac cccatgggca tcagaagcag ccatctgcac gagattatgt ttgaccatcg
     2101 ttttggctgt tttctggatg tctttgccaa ataccccaac ataacttggc gcagtgagtt
     2161 gggccaaaca tcccatatct aggaacggaa gcaaatgatt tgggtcttcg cgaaaatgca
     2221 cgttgcgttc tggatggacg attactggtg tctttcctag ttctaacaac tcaaaaaaaa
     2281 gccgttctgc atataacggc acttccagtg tcggaaattc gatcagaaga tatgtatcat
     2341 ctaaatctgt aaataatatt tcatctcggt ggatgtcttc gatgagcgtt ccggtaatac
     2401 gtacttcttg tccttccaat aacgtcaaag gcaactgtct tttctctaat tcggcttgta
     2461 acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca ttattgtggt
     2521 gtggtgtgca taaaatatgc gtgatgcctt gctgaatcgc tttttcagcc attgcaatac
     2581 ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag gatatggcaa tgtagatcga
     2641 tcatttctct tgtctcctct cttcttattc cttaatttcc gtagtaataa ccagcatctt
     2701 tgctatgttc tgctccgttg tacacaacac ctaagactcg tgcttgtacc atctccagca
     2761 gttcttttgc tttagtcaag gattctttac gtgacatatt ttcacgtacg accaataaag
     2821 tgccatccgt ttttaaagac atgatctgtg catctgttac ggctactact ggtggcatat
     2881 cgaatatcac cacgtcaaat aactgtcttg cttcggatag gatctgatcc attcttggcg
     2941 agcttaacaa ttctgatggg ttgggtggct taggtccact aggcaaaatc gacaagttct
     3001 cgactggcgt tcgttggatc acatctgcta cacttcctga tgaactaagt gctgtactta
     3061 atccactggc attgttcagc tggaatgtct tatagacgac gggtttacgt aaatctgcat
     3121 cgactagtag aactcgttga cctgatttag caaaaacgac tgcaatgttt gctgcagtgg
     3181 tggatttccc ttctccagga cctgatgaag tcacgacaat cgttttgatt tgctgaccag
     3241 ctgcggaggc aaactggata ttcgtccgga tcgtacgata ttgttcggcg attggtgaag
     3301 atgggtttgt taatgtgatc aaactgactg catgagtttg tgctgctttt tgtttctgtg
     3361 ttcgtgccat ttttcttcct cctagactct tgagcggctt ctgcgtgatg gggctgtttt
     3421 atcgtttgta gcttttggtc gtgcaggagc tgttcctttt ttacggatac cagcgttcaa
     3481 ttctttcgct gacatttctg gaactgttcc taagattggg aatcctaatg tatctgtcac
     3541 atatttttca tctttcactg tccgatcgag taattccaat aagaatgcca gacctacacc
     3601 taccatcatt cctaacatta agccagtcat taaattcaat ttattatttg gcgaaacagg
     3661 tgtcgtattg gcaactgcac ctgaaatgat cgagatttta tcaacattca tcacatcttt
     3721 tgcgttctct tggaaaactt gtgaagttgt gttagcgatc tgttgagcag ccactgcatt
     3781 cgtacttatt gcctgaatcg agaacatttg tgaattttct gcctgattaa ctgagatggc
     3841 atctttgatt tgttcagcag tcattttcac attgtcttct gtttctaaac gatctttgac
     3901 ttcattgagc actaaatcac tgacgatcat atctttatac gtattgatca tttgcaggtt
     3961 cgtattgaca tcattgacat ttgcagtgtc tgattgtggc agtgtcacga tcaattgtgc
     4021 ttgtgaactg tattttggtg tcaagatgaa aaatgttacg attcctgaga tggccaagcc
     4081 agcaaacatt gagatgatga ttattaccaa gtgtttgcgt aatgtttcaa aaatttcttt
     4141 caagctgatt gtctcttcca ttttttctcc actttcctat ttattctcta gattcatttg
     4201 atcttttaat tcttttttca cacgatcaag ttcttgatca ctgacttttt gataggagat
     4261 attatcttgc atgaagcctt ctccttgcag ctgatcttgc ttaatcgtac caaatgcctc
     4321 ccgataatct aaagcaattt ttttcatttc atcaaagttc atgtctgttt tcatattcga
     4381 ctctagagca ttcaaaacgg attgatagtt gctgacaccg tttagactca atactttttt
     4441 cgctacgcct tctacgatct ttctttggcg cccttgtcta ccgtaatcac catttgggtc
     4501 ttcataacgc atacgagaat aagataatgc ctgatccccg ttcaatgtaa ttttaccgat
     4561 tggaaagtca tagccatctt gtgagaaagt gaggtcatta ttgacttcga taccaccaac
     4621 tgcatcaacg agttccttta gtcctttcat attgatagag acatagtgat cgattgggat
     4681 gtctacatat ttttgtacgg tatccatcgc catggcagct ccgccgaatg cgtaagcgtg
     4741 gttgatcttg tcttttgtgt cacgacctac gatgtcgaca tacgtatctc gaggaatgct
     4801 gacgatcgtt gtctgtttgt cttcaggact gactgtggca accatcatcg tatctgatct
     4861 tccttgatcg acccgaccta aatctccagt atcaatcccc atcaatagaa cagagaaaga
     4921 ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt ccactgattc
     4981 gtaagttttc tcaatagaat gtgagagatc cagatataat tttgctccta ctcctacaat
     5041 tgctaatact aatacgataa gaactgctaa aataatcaaa aatatttttt tccctcttga
     5101 ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga atcaacgttt
     5161 taactgtcaa tagcttatat tgctatagcc catttgctga gcaacagttt gtgcttttgt
     5221 tgcgtatgcg gctcttattt tttcattccg gatctgctct atttgactga gcgccgtcaa
     5281 atattgatcg taagtagcgg tagctgttac ttgaccgtct ttcagcatcg tatcgaatag
     5341 ctgatctagt tgcgtgactt gtgcaatttg ggtatcagcg aatcctagat attgcataac
     5401 gactgttttc caagcactat ccttaaaaaa gctcatattt tcacgtacat gggcgacatc
     5461 tgcacttgcc aacttttctt tgatgatgac atcatccaca gcttgctgcc agtttgatac
     5521 gtttttagta aataatttat tgacagccgt ctggatcttc agttttgatt ctgcatcctc
     5581 taactgcgtt aagactgctt tcttttcttc ttggatcgca gcagcttctt taggtaattc
     5641 tgattccttg atgtcataat catctgccga aagtttgata gaagatagtt tatgtgacaa
     5701 atttgaaact gtttcttctg ttatttcagg atttaggaaa attttttctt ttgtactgta
     5761 cagcttatct acttcttttg ataaagaggt cacggtgtct ttttgattgg tgatggcaga
     5821 ttctgcgtaa ttgatccgtt gatgatattg ccgctgatta ttgagatggt atccaaacaa
     5881 gcaaatcaca atagcgcctg tgcctaccgc cacagtagcc aataacttga cgaacggctt
     5941 ttccatagat tcactcctaa atattgatga tagataagta aagatgtgat tcattcacat
     6001 cagaatgagc gctttttatt ttaaaagcat aattaaacta atatattcat agaaatattt
     6061 ataaacaggt tttccctata taatcatacc acatacagtt tttttgacta gggaaa
//
LOCUS       DANKBF010000112         5689 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000112 DANKBF010000000
VERSION     DANKBF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(488..1297)
                     /locus_tag="PWY89_002773"
     CDS             complement(488..1297)
                     /locus_tag="PWY89_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2067751.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="PWY89_002774"
     CDS             complement(1297..2043)
                     /locus_tag="PWY89_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2067752.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="PWY89_002775"
     CDS             complement(2061..2528)
                     /locus_tag="PWY89_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2067753.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="PWY89_002776"
     CDS             complement(2528..2938)
                     /locus_tag="PWY89_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2067754.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="PWY89_002777"
     CDS             complement(2949..3962)
                     /locus_tag="PWY89_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2067755.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="PWY89_002778"
     CDS             4179..4913
                     /locus_tag="PWY89_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2067756.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="PWY89_002779"
     CDS             4897..5607
                     /locus_tag="PWY89_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2067757.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DANKBF010000113         5659 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000113 DANKBF010000000
VERSION     DANKBF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(1014..1304)
                     /locus_tag="PWY89_002780"
     CDS             complement(1014..1304)
                     /locus_tag="PWY89_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067758.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="PWY89_002781"
     CDS             1706..1942
                     /locus_tag="PWY89_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067759.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="PWY89_002782"
     CDS             1949..3901
                     /locus_tag="PWY89_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2067760.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PWY89_002783"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PWY89_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2067761.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="PWY89_002784"
     CDS             complement(4797..5489)
                     /locus_tag="PWY89_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2067762.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1848 a          982 c          906 g         1923 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgca
//
LOCUS       DANKBF010000114         5481 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000114 DANKBF010000000
VERSION     DANKBF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="PWY89_002785"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="PWY89_002785"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="PWY89_002786"
     CDS             complement(580..1353)
                     /locus_tag="PWY89_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2067763.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="PWY89_002787"
     CDS             1497..1847
                     /locus_tag="PWY89_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2067764.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="PWY89_002788"
     CDS             1828..2544
                     /locus_tag="PWY89_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2067765.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="PWY89_002789"
     CDS             2544..3992
                     /locus_tag="PWY89_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2067766.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="PWY89_002790"
     CDS             complement(4062..4571)
                     /locus_tag="PWY89_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067767.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="PWY89_002791"
     CDS             complement(4561..5286)
                     /locus_tag="PWY89_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067768.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DANKBF010000115         5342 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000115 DANKBF010000000
VERSION     DANKBF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(61..279)
                     /locus_tag="PWY89_002792"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="PWY89_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="PWY89_002793"
     CDS             complement(545..739)
                     /locus_tag="PWY89_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067769.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="PWY89_002794"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="PWY89_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="PWY89_002795"
     CDS             2091..3131
                     /locus_tag="PWY89_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2067770.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3955..4287
                     /locus_tag="PWY89_002796"
     CDS             3955..4287
                     /locus_tag="PWY89_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2067771.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="PWY89_002797"
     CDS             4906..5247
                     /locus_tag="PWY89_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL2067772.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1771 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtgatat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg caagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgtttt gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattaat
     4861 ataaagcata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag gggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgacgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     5341 tt
//
LOCUS       DANKBF010000116         5341 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000116 DANKBF010000000
VERSION     DANKBF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(11..1942)
                     /locus_tag="PWY89_002798"
     CDS             complement(11..1942)
                     /locus_tag="PWY89_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2067773.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="PWY89_002799"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="PWY89_002799"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2067774.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="PWY89_002800"
     CDS             complement(2871..3623)
                     /locus_tag="PWY89_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2067775.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="PWY89_002801"
     CDS             complement(3782..3991)
                     /locus_tag="PWY89_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067776.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="PWY89_002802"
     CDS             complement(4002..5330)
                     /locus_tag="PWY89_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067777.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         1501 a         1152 c          932 g         1756 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 c
//
LOCUS       DANKBF010000117         5312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000117 DANKBF010000000
VERSION     DANKBF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="PWY89_002803"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="PWY89_002803"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2067778.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="PWY89_002804"
     CDS             complement(1298..2134)
                     /locus_tag="PWY89_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067779.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="PWY89_002805"
     CDS             complement(2318..3535)
                     /locus_tag="PWY89_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2067780.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3863)
                     /locus_tag="PWY89_002806"
     CDS             complement(3684..3863)
                     /locus_tag="PWY89_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067781.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3918..4001
                     /locus_tag="PWY89_002807"
     tRNA            3918..4001
                     /locus_tag="PWY89_002807"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..4819)
                     /locus_tag="PWY89_002808"
     CDS             complement(4241..4819)
                     /locus_tag="PWY89_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067782.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4825..5208)
                     /locus_tag="PWY89_002809"
                     /pseudo
     CDS             complement(4825..5208)
                     /locus_tag="PWY89_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac at
//
LOCUS       DANKBF010000118         5199 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000118 DANKBF010000000
VERSION     DANKBF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            23..694
                     /locus_tag="PWY89_002810"
     CDS             23..694
                     /locus_tag="PWY89_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2067783.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="PWY89_002811"
     CDS             753..1193
                     /locus_tag="PWY89_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067784.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="PWY89_002812"
     CDS             1205..1873
                     /locus_tag="PWY89_002812"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2067785.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="PWY89_002813"
     CDS             1937..2560
                     /locus_tag="PWY89_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2067786.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="PWY89_002814"
     CDS             2616..3002
                     /locus_tag="PWY89_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL2067787.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="PWY89_002815"
     CDS             3215..4606
                     /locus_tag="PWY89_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL2067788.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1727 a          861 c         1095 g         1516 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aataagaggt
     4741 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     4801 ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
     4861 aggtcgtgag cctgccgttt cactctgagt cacaaagagc aatttcaaaa aatagcttct
     4921 catgtttgtt gaatattgtg tcttgtgacc gaggagttgt caggagaaca ccgtttacca
     4981 gaggttgtga cttgttttta gtcacgacct ctttttttat ttattccata taaatacaca
     5041 ttatttattc cgaaaagtga aagcgcaaat aacaaaaaca accaacttat ttattattaa
     5101 agaaaagcgt atttaagtag gacgattctg ttgagagctt tcgtagattt tcccattttc
     5161 ctatggtaaa ctgacaaagt tgagaatgtt agaacagat
//
LOCUS       DANKBF010000119         5189 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000119 DANKBF010000000
VERSION     DANKBF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            137..748
                     /locus_tag="PWY89_002816"
     CDS             137..748
                     /locus_tag="PWY89_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2067789.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="PWY89_002817"
     CDS             821..1750
                     /locus_tag="PWY89_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2067790.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="PWY89_002818"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="PWY89_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="PWY89_002819"
     CDS             complement(2693..3871)
                     /locus_tag="PWY89_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2067791.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3969..4067
                     /locus_tag="PWY89_002820"
     CDS             3969..4067
                     /locus_tag="PWY89_002820"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2067792.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="PWY89_002821"
     CDS             4098..4499
                     /locus_tag="PWY89_002821"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2067793.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5189)
                     /locus_tag="PWY89_002822"
     CDS             complement(4706..>5189)
                     /locus_tag="PWY89_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2067794.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1638 a          879 c         1004 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 gtgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DANKBF010000120         5176 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000120 DANKBF010000000
VERSION     DANKBF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(136..1098)
                     /locus_tag="PWY89_002823"
     CDS             complement(136..1098)
                     /locus_tag="PWY89_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2067795.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="PWY89_002824"
     CDS             complement(1100..2539)
                     /locus_tag="PWY89_002824"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL2067796.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="PWY89_002825"
     CDS             2724..4670
                     /locus_tag="PWY89_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL2067797.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="PWY89_002826"
     CDS             4934..>5176
                     /locus_tag="PWY89_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2067798.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DANKBF010000121         5152 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000121 DANKBF010000000
VERSION     DANKBF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(52..561)
                     /locus_tag="PWY89_002827"
     CDS             complement(52..561)
                     /locus_tag="PWY89_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2067799.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(673..1671)
                     /locus_tag="PWY89_002828"
     CDS             complement(673..1671)
                     /locus_tag="PWY89_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2067800.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1872..2456
                     /locus_tag="PWY89_002829"
     CDS             1872..2456
                     /locus_tag="PWY89_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2067801.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(2533..3726)
                     /locus_tag="PWY89_002830"
     CDS             complement(2533..3726)
                     /locus_tag="PWY89_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2067802.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            3790..4170
                     /locus_tag="PWY89_002831"
     CDS             3790..4170
                     /locus_tag="PWY89_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2067803.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            4244..4606
                     /locus_tag="PWY89_002832"
     CDS             4244..4606
                     /locus_tag="PWY89_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067804.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1684 a          891 c          958 g         1619 t
ORIGIN      
        1 aaaaaagaaa gaactcgtct aaatagacgt tttctttctt tttttgttga attattgagt
       61 aaggcgactt cttttttctg gttctttgtg tgctaatcga ctggcagcag cagcggcgat
      121 cgctccgacg atatcatcta agaaggtatg gacatgaacg ccatcgtggc tgtttagttc
      181 ttttaaaata ccaggtttca ccttgtcgat ataaccataa ttcgtaaagc cgatcgtacc
      241 gtaaacattc acgattgaca aggctaggat ctcatcgata ccgtaaaggc cttcgtcatc
      301 ttcgataata tgttgcaacg gactcatcaa ttttttcttt tctgctaata tatctaattg
      361 gattccagtg ataatggcgt tatgtacttc acgctttgtt agcacgctgt tgacactttc
      421 agtacaatcc gccaaagtca aatcagaaat atagtctttt tgtaaaaaca tgacaagttg
      481 tgcgatatct tctacagtta cgccacgttc cttcaatagt tctcttgcct tttcttctaa
      541 agtctcagtt ttgacgacca tattattgct ccccctttat tttttctttc tctatttcat
      601 gttaacggaa catgaaaaaa atgcaagaaa ctgctttcgg gtttaacttg aaatgcaaag
      661 agatataaaa aatcaactag gacgaagatt tttatcgtat aagcttgtgc tgtgaccagg
      721 ctgagttcgt tcctttggat taatatagtg taaagcgttg ttcactgcag taggtgcttc
      781 accgagccca gtagcaatca gtttcacttt tccttcatag gtacagatgt caccagctgc
      841 ataaacgcct ggaatagaag tagacatatc tgattttaca gtaatggcat ttctggtact
      901 atcaagtccc caagaagaaa gatgttctaa attagaagag aatccataat tgacgatcag
      961 agaatcaatc atcaaatcaa tcgtctcgtc acttttcact ttttttaacc ggatatctgt
     1021 cagttcccca ttattgcctg ataatccatc gatgagatag ggagtaagca agttgacaga
     1081 tgaagatttc aagcgtgata cactatgttc gtgcgcacga aattccgggc gacgatgaat
     1141 caagtaaact tcgcttgcaa taggctctaa catcaacgtc caatcgatag cagaatcacc
     1201 acctccagca atagcgactt tttttccagc atacttcatc aaatcattca cgaaataatc
     1261 caatccgtgg ttttcaaatg attcagcatt atctaaattc aattttcttg gctggaatga
     1321 accatttcct aaagctaaaa tgactgcttt ggaataatgg atgcctttat ttgtagtaat
     1381 ctcgataact tcgtcttcac gagtaagcga gagaacttct tcttttaaat ggaatgtatg
     1441 gttaaaagta gttaattgtt tttctaattg atcgattaat tcacttgctt ttattgctgg
     1501 atacccagga atatcgtaaa tatatttttc gggatacaaa gttgctaatt gaccaccaag
     1561 ttgaggtaga ctgtcaatga tttttgtttt agcttggcgc attcctgcgt aaaaagcggc
     1621 aaacatccct acaggacctg caccgatgat tgtaatgtca tagatttcca taaaaattcc
     1681 tcccttttca cactatagca gaatttaatt gagaatgctt ctcaaaaatg tttgaattcg
     1741 aaatcgaaac ggtaagttat agaatgtatc tagtgatgaa aaataaaaga aaacaaatct
     1801 agtattttta cgagaggtta ggtattatgt tattattatt atgaagtatg caaatattag
     1861 gaggaaacaa tatggcattc ccacaactag atttaaaaag tgaaaaagga ccaaaagctg
     1921 ttatcaaaac aaaccgtggt gacattactg ttcaattatt ccctgaacta gcaccgaaga
     1981 cggttcaaaa tttcattgaa ctttccaaaa aaggttatta tgatggcgtg atttttcacc
     2041 gcgttattcc tgatttcatg atccaaggcg gcgatccaac tggaacagga atgggtggcg
     2101 aaagtattta tggcgaaagt tttgaagatg aattcagtcg tgaactgttc aaccttcgcg
     2161 gagctttatc tatggcaaat tcaggtccaa acacaaacgg aagccaattc tttattgtaa
     2221 ataacacaaa tgtgccagct aatatgcttg gacaactaga aggtgctgga ttcccatcag
     2281 aaatcattga agcgtataaa ggtggcggaa ctccttggct agatttccga catacagtct
     2341 ttggacatgt actagaaggg atggacacag tagatgaaat cgcaaatgta caacgtggtc
     2401 cacaagaccg acctgtacat gatgtagtga ttgaaacaat tgaaatttca gaatagtttg
     2461 gtatggtacg gagtaattat gaaaaaggac gaaacctagt aaggtctcgt ccttttattt
     2521 tgaataaatg atttaatcga ttgtctctaa agcatacttg attcgattaa gtccttccag
     2581 taaagtttct tctggacaag caatattcaa gcgcatatgc ccatgaccgg aaggaccaaa
     2641 cgtgatgcca ggatttaata cgacttttcc tttccgaaca agagtgttct ccaattcttg
     2701 gtcgtctgaa atataggcgg taaaatctaa ccacattaag taagttcctt cagggttcat
     2761 tattctcact ttcggaagat actgttcaaa aaactgatta actgtttgaa catttttttc
     2821 tagataagga atcagttggc ttaaccattc tccacccgtc tcataagccg cttgggttcc
     2881 aatcagtcca aaagtgttga tttcgtgctg ttggttcatc actaaatgtt tttcaaaatc
     2941 agctttcatc tctggatttt tgataaatac cattgaattt ttgattgcag ctagattgaa
     3001 agtcttggtt gcggaagtaa aagcaactag tagatcatct aaattaggat caatcgttag
     3061 catggaagta aatgtatgat cgaataaagt taaatcttga tggatctcgt cactaaacag
     3121 aatcacttga tgttttaaac acaattcgct taactgtttc aattcttctt tgctccaaac
     3181 acgccctcca ggattgtgag gattgcataa aatcattgct tttacattgt tttcttcgat
     3241 ttttttctcc atgtcagaca agtccatgat gaaatgatta tttttttcta gcaaagagct
     3301 tcgaacgatg tgacgttgat ttgtttctat aatagaggaa aatggaggat aaactgggtc
     3361 atgaatcaaa atagaatcgc ctggttttgt aaaagtttgt actgctgcag ctaaactggt
     3421 taacacacca cttgtaaaaa cgatattttc tttcgtcaac tccacagcat ggtgttggcg
     3481 ttcccaatga atgatagcct gatatagttt atcagacaga gtggcataac caaagattcc
     3541 atgtttgatg tattcactaa acgctttagc taccccaccg ggagacaaga agtccatatc
     3601 tgctacccac aaaggaagta agtcatccat ttgatatgtc tcagcaattg cgtcccattt
     3661 tacactttct gtatcttttc ttgaaatctg tttatcaaat tcgatcatct taagacctcc
     3721 agtcattttc gtcgcttctt tttttgagct atttacagta taatgataaa tgcgaagaga
     3781 gggaagtaga tgacttataa aattggacaa ataattgaag gaaaagtgac agggatacag
     3841 ccttatggcg cattcgtttc tcttgataac gagacgcaag gacttattca tgtttcagaa
     3901 gtacaatccg gttatacgaa aaatatccat tcattgttaa aagtcggaca gccagtaaca
     3961 gttcagatca tcgacattga tgaatattcc aaaaaaatca gtttatcttt aagaacgtta
     4021 gaaaaaacaa atccagctgt tccatatcgc agaaaaaggt atttcacgaa taaaaatcgt
     4081 aagatcggat ttaccactat tgcggagcag ttacctattt ggatagaaga agcattatct
     4141 gaactagaac ggaaagaaaa taagaaataa aaagtgtatt ttttctgaaa atgtgctaga
     4201 atttttatgt aagccataga acaagttgat cgaggtggaa attttggcaa gcaaaagagt
     4261 tgctggtaca attatgttgc atttggagga tggtactaaa aaatttttgg tccattcaat
     4321 tgatgacaag ttggaatttg cgttagcgga actttctgaa ggaaagactg gtttagcgaa
     4381 tatacttaat ttcttaaaag aaatagttca tatcgatgtt agcaagatta gtttgatgga
     4441 attgacaaat acacatatta aagaggaaaa tgttccgttg tttgtctttg aaactgaaca
     4501 aaagaatcaa agagaagaat taggcgaagg atatatttgg gaagaaccag gtcaaatgcg
     4561 ctctgttctt ggaacttacg aagtagaggg agtgcctttc ttctaaaata cagattaagc
     4621 gaacaaagaa gtttgaattt ttttcaaact tcttttttta ttgtttggtc caaaaaatac
     4681 aatgaaatgt tctgctaaag atagtttttt agtgcatttt attttcaaaa tatgttgaaa
     4741 acggttgtat aacgaataat aataattgat cgaaaaatca tttcacaaag taatgcttaa
     4801 tttatagttg accaaggaag aaatacatga taaatttaac agcgttgatt ctgaagtgga
     4861 tgttatatca catttctaaa aaatttaaaa aatagttatt gacatgttac taacgacatg
     4921 ataagataac tgagtcgtta aaaaagaacc acttgcgaag cttgattcta tcgaaaaaaa
     4981 cttcaaaaaa gttcttgaca aataacaaac gataagttat gataaatgag ttgctgaaag
     5041 atttcgaaaa acgaaatcga aaaaaacttt taaaaaagag ttgacaatcg cttgtctctc
     5101 tgatatgata taaaagttgc tacggcagcc aaaaacgaat cgggaaacac tc
//
LOCUS       DANKBF010000122         5137 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000122 DANKBF010000000
VERSION     DANKBF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..447)
                     /locus_tag="PWY89_002833"
     CDS             complement(<1..447)
                     /locus_tag="PWY89_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2067805.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(754..1851)
                     /locus_tag="PWY89_002834"
     CDS             complement(754..1851)
                     /locus_tag="PWY89_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2067806.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1959..3986)
                     /locus_tag="PWY89_002835"
     CDS             complement(1959..3986)
                     /locus_tag="PWY89_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2067807.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4131..4862)
                     /locus_tag="PWY89_002836"
     CDS             complement(4131..4862)
                     /locus_tag="PWY89_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2067808.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1040 c          813 g         1828 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat attgttataa aaagagccca accatcctac
      721 aataataaaa atttactatt tgttatctta accttacttt tctttctctt ctttttttct
      781 caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag ctactaagat
      841 ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt ccttggctac
      901 ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt tgatcgtagt
      961 atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa cagtcattgt
     1021 aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa aagaaaaatt
     1081 tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt cagaatgtcc
     1141 tttttttgta atttttgttt caataactac ttgattgata taggtttttt gatcattttg
     1201 aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt catgaagagt
     1261 aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa tataggtgta
     1321 ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt ccgtctggat
     1381 ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg gcatcctgac
     1441 atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg gatagctcac
     1501 ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt ttccgtattc
     1561 aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt tcacttcttt
     1621 ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat caagttgaag
     1681 atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg tcactgaaaa
     1741 attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa taactaggag
     1801 tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca tatgtaagag
     1861 aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc tgtgcccttc
     1921 ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg gacatctgca
     1981 agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac aggaatattt
     2041 attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct ttcacccttt
     2101 tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt ttcagaggta
     2161 acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt ctcttgtttc
     2221 aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc acctgaaaac
     2281 tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt gacatattga
     2341 ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc ctctgtattt
     2401 tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc ttcaatatca
     2461 ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat ttcagcaata
     2521 tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt cagctttgcg
     2581 atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag gtattcaaaa
     2641 tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa ttcgattatt
     2701 aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt tccggttatc
     2761 aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg aatagttaat
     2821 tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc cccctcgacc
     2881 ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat ggccccataa
     2941 tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca
     3001 gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca atttacttct
     3061 gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagcgacc gacaccttcc
     3121 aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg
     3181 ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga ataattatta
     3241 ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc agcagttatc
     3301 tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc aatcgagagc
     3361 gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc catacctggc
     3421 gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa tgtaaaggtc
     3481 atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt aaatgtaaaa
     3541 tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga cctttctgct
     3601 gggttcaact gcagtaactt cccattttcc aatatggacg tgctagtacc gataggtgtt
     3661 tgaaaaattt tatcgataga aataatattg ggatcgtcag gcagctcaac cgcagaaatt
     3721 ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt ctacgttgtc agtggtctct
     3781 ccactactcg aaatacttaa atcctcactg ctttcttgtg tctggttgga ttctattaaa
     3841 ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc tatttccatg ctccaactga
     3901 ttcaattcca aagagtaccc tatgatcgga aagatattag tcactaagag actgcttaaa
     3961 atgagcacga tcctcctctt tttcaatata tcttttcctc ctgatcgtta aattcctaga
     4021 tgaactattc gtatccaaac aaatttagcc tagcctttga caatttttgc tcgattacct
     4081 cgcaagacaa tatgactacg tatttatttt taacagttct tttcagtaaa ctagttgtta
     4141 ggtgtatctt ctaatgtcca aactaattta gtggaatatt gttgcgctaa cttgacagat
     4201 ttaccgggta ccgaaagctg taccgcatct tgattttcat tttcatttgc accaaatcga
     4261 tagacccaag tccccacacc tgttcctgat ttagcattaa ttgctaacgt attgtttcct
     4321 cccggtgtca acgttaattc agaattcacc gtttgcggcg tatatgttgg atcaaccaaa
     4381 gaaacagcct gccctttcgt aaatttcagc tgtgctccaa ctagttcatc cccactggca
     4441 gttttaaact gttctggttg agagacactt aatttccacc cagataaggt acttctccta
     4501 tctgtcactt ggacatatgt cggttttcta gacccatcgt tcatcacatc agcagctgca
     4561 taatacgttt tatccgcagt agaaattttt tgagtcccaa atttgaattt tgatccataa
     4621 tcaatcgata atgcccctcc tgttccaggg cttggaggat ctgatggatc gacaggttta
     4681 ttgggatcag gattctctgg atcaacagga ggagttacac cctcatctcc tgcttcaaat
     4741 gtaatcgttc cttctgtgtt atattccttt gggtatactt cagttgcaga aacaaaagtc
     4801 gtacttgcta ccatgctgaa taacatcatt gctgctgtta atacgatttc ttttttcatc
     4861 attgacattc ctctttcttt atttactaaa catatcagtc gattggatta atccatagca
     4921 aaattctgtt ttacgatact tatgaaataa aagaacatag acctatattt ggtcctatgt
     4981 atcgattagt tataaatgat tatcagcccg atttgatttc tagtttttat tgcaacttga
     5041 tgcaaacaca atctagaaat acagctgtca tagcctttaa aataattcgt aagtccccct
     5101 cctatctatc aaaatgtgac ttgacgagag tgtaaaa
//
LOCUS       DANKBF010000123         5132 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000123 DANKBF010000000
VERSION     DANKBF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            57..1991
                     /locus_tag="PWY89_002837"
     CDS             57..1991
                     /locus_tag="PWY89_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2067809.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2448..2864
                     /locus_tag="PWY89_002838"
     CDS             2448..2864
                     /locus_tag="PWY89_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2067810.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2878..3444
                     /locus_tag="PWY89_002839"
     CDS             2878..3444
                     /locus_tag="PWY89_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL2067811.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3467..4453
                     /locus_tag="PWY89_002840"
     CDS             3467..4453
                     /locus_tag="PWY89_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2067812.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4608..>5132)
                     /gene="scm"
                     /locus_tag="PWY89_002841"
     CDS             complement(4608..>5132)
                     /gene="scm"
                     /locus_tag="PWY89_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2067813.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         1547 a          948 c         1135 g         1502 t
ORIGIN      
        1 ctaaaagaat tatcatatat tcattatata gatagtctat aaggagcgga tgatcaatgg
       61 caaagcaaaa tattgtcgtt gtaggagcag gatatgccgg tgtttcagct acaaaatact
      121 tggcaaagaa actgaaaaaa gaagatgtag caattactct gatcgatcgt cattcgtacc
      181 atactatgat gaccgagttg catgaagtag ctggcggacg tgtagaacca gaagcaatcc
      241 aatacgatct gcagcatttg tttgctcgtc aaaagaatgt acaacttgtc acagacactg
      301 taatcggtat cgataaagaa cgaaaaatcg tcaagaccaa attaggttcc tatccttttg
      361 atcagctgat ccttggaatg ggcggagagc cgaatgactt tggtacacct ggtgtcaaag
      421 cacacggatt caccctttgg tcatttgaag attctgtaag gattcgggaa catattgaga
      481 agacagtagc aaaagctgca ttggaaccag atgatgaact gcgtaaagcc atgctgactt
      541 tcgttgtctg tggttctgga tttaccggta ttgaaatgat tggggaactg atcgactgga
      601 aaaatcgcct agccaaagat tataaattag atccaaacga aatcacactg atggtcgtag
      661 aggcaatgcc gacgatcttg aatatgttgg atcgaaatga tgctgcaaaa gctgaacgct
      721 atttgcgtaa gcacaacgtg gaattattat tgaatgcacc aatcgtagaa gtggctgctg
      781 atcatatcaa attgaaagac ggacgcaaca ttccgactca cacactgatt tggacagctg
      841 gtgtcaaagg aacttctgac gctgctgact ttggattaga atcagcacgc ggacagcggt
      901 tgatcgccaa tgaatatatg caggcaaaag gctacgaaga taaaaacatc tatatcattg
      961 gtgatttagt cttttatgaa gaattcccag gaacaccgac accgcagatc gttcaagctg
     1021 ctgaacaaac aggacacaca gccgcagcga atatcgtggc ttcgatcaaa ggcggagaaa
     1081 agcataaatt taaaggaaac tatcaaggat ttatggtttc tattggtgcc aaatgggggg
     1141 tagctaacct atttgataag atccatttaa gcggattttt agctatcgta atgaaacata
     1201 tcgtgaattt gaaatacttc tttgatattc gttctggtta ttatatgttc caatatttaa
     1261 tgcatgagtt tttccatatc aaagatgatc gcaacgtgac tcgaggacat tcttctcgtt
     1321 acggcaatgt cttatggagt gtgccacttc gtattttcta tggtttagtt tggttcgttg
     1381 aagcttccaa aaaaatcatt gggaatggtg aagtgttcaa accaagtact tggtttggtg
     1441 aaggatcatg gttcactgga aacagtgtgt tcccttggac atgggaatac aatgtaacag
     1501 atgttgccag tggtgcttct gctgctggtg atgcaaccac agctgccagc ggagcagggg
     1561 caagtgccgg agaagcagcg actcaagctg cacattttgg tttgagttat gcatacggtg
     1621 aagaaccaat ggctgtgatt gagaagtcac cagactggtt cacaaagatc atgcaagtga
     1681 tcatgccaaa caatgatgta gcaatgttct tccaaaaatt catggtattc tttgaaattg
     1741 ccttggcatt agcattgatt gctggattat tcacatggct atgcagtgcg acaacgattg
     1801 tattggtgat cacgttctgc ttatcaggca tgttttactg ggtaaacatt tggttcattt
     1861 ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa
     1921 ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg
     1981 gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg
     2041 agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa
     2101 gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt
     2161 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat
     2221 tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata
     2281 aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag
     2341 ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt
     2401 ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag
     2461 aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg
     2521 ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa
     2581 aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag
     2641 aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag
     2701 atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag
     2761 gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag
     2821 tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg
     2881 agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt
     2941 ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta
     3001 gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacgagacag cttcttacta
     3061 ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac attcctttat
     3121 agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca
     3181 aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag
     3241 cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt
     3301 tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga
     3361 catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg
     3421 agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc
     3481 aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga
     3541 agaaactgtc cgcctgaaaa acaaacctgt cgaacaagca atcaaagaaa cgatccatgc
     3601 cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca
     3661 agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact
     3721 gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag
     3781 tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa
     3841 actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga
     3901 aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt
     3961 aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc
     4021 tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg
     4081 actttctatc gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag
     4141 ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt
     4201 gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc
     4261 aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga
     4321 agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt
     4381 acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga
     4441 tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta
     4501 cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag
     4561 agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat
     4621 ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt
     4681 gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca
     4741 ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt
     4801 gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg
     4861 cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt actgtcactt
     4921 gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct atcacttgtg
     4981 gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc acttgttgtg
     5041 gtagatgtat ccgtactgtc gcttgaagct gacgtgtccg tgctatcact tgtggtagag
     5101 gtagtatctg tactgtcact tgaagctgat gt
//
LOCUS       DANKBF010000124         5088 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000124 DANKBF010000000
VERSION     DANKBF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(205..660)
                     /locus_tag="PWY89_002842"
     CDS             complement(205..660)
                     /locus_tag="PWY89_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067814.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1269..1685)
                     /locus_tag="PWY89_002843"
     CDS             complement(1269..1685)
                     /locus_tag="PWY89_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067815.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1687..1968)
                     /locus_tag="PWY89_002844"
     CDS             complement(1687..1968)
                     /locus_tag="PWY89_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067816.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2415..3383)
                     /locus_tag="PWY89_002845"
     CDS             complement(2415..3383)
                     /locus_tag="PWY89_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2067817.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(4550..4705)
                     /locus_tag="PWY89_002846"
     CDS             complement(4550..4705)
                     /locus_tag="PWY89_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067818.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4724..>5088)
                     /locus_tag="PWY89_002847"
     CDS             complement(4724..>5088)
                     /locus_tag="PWY89_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2067819.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1595 a          839 c          839 g         1815 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgatttcta attggtctag cttaaacact tcagccaatg cttgatttta tgcacttttc
      901 ttttttcatt tctttgtgca ttttgaccgg tcatttttta gagatttgtt atttttgtga
      961 gcatttgata tgctcttttt atacctcaat ctcttctttt ctaatgttaa tgaataggat
     1021 aatctcattt ttagctgctc gtgagagcag tctttttttg tttcagggta cgtcctattc
     1081 tttggatttc cgcgctatta aataaccgat acttggggat tggggtagcg ccccaaaata
     1141 caaaaagaag aagttgcata agcggatttt tctttttttt cgcaagttgg taccaacttg
     1201 caaatcttgc tggaagactg atccctaaaa agaaatcgag gaattttttt agggatcggt
     1261 ttcttttttt atctttttag aaagtttgtt gggtcaaaat ttccagaagt aaaggtttga
     1321 ttggtttctt tttgacgatt tttgggttga tttcgtcggg aaggattgag ttgattttca
     1381 attttttctt tcacttcttg atacacttta gaatttgtac cgataagaac accagtatct
     1441 tttagatata aatcagcaaa ttctgccatg atttctgtca tgatagaaat ttcttttccc
     1501 atggcattga gttttgcttg gttactggtt gtgattgtat tggtatcagg ttcataactc
     1561 atacataaaa aacgaatggc ttctgaataa ttgttgacgt ctggattttt ttcgatgatg
     1621 gaatctaaaa cgtcaacgtg actgggataa agggttacgt gtttttgaac agaagttttt
     1681 ctcatgttat attcctccga taagtgtttc aatgagttgg ttgtttgtgt gaacatagtc
     1741 attcagtatg ttcgtatttt ctgtttgaat ggcaataaat ttttcgagta ataaatcgaa
     1801 ttgatctttt ttgtaattca taagttgacg gtcaaaatca ctttcgatta gaacttttag
     1861 atatttattt cgactccatt ttttgcccgt tttggttgtg agttcctgac aacgtttatc
     1921 tattcttgca ataattttgg gattgatatt tcgaactaaa atgtccatgg aaattttcct
     1981 ttcgtatcag cgtataattt atgtatatca tacaaatata gtatttgtat agctattttt
     2041 cgccaaaaca ctatacatag cctagccaca atggcaaaaa agtgatagca aatttttttc
     2101 tgagaaacgt gataggcaca gcctatctag caccttttgt aagaaagtga taccacgttt
     2161 ccttatgctc tttcaaaatt cagaaaaatc agctgtaaaa cgagtgcagt ccgagtaaga
     2221 ttcaacgaaa aaacggggat tggggcgcca ccaaaatatc gcaacatggg tccatgttgc
     2281 tctgctcgca aaagcaaaaa gaagaataaa accagcaatt taaattaata aaaattgctg
     2341 gttttattgc cagaattgcc accatttttt ctccgtcttt gattgttttt tctgtcataa
     2401 aatctaaaaa cccctcaatc tttgaataag ttttgaagat tgtctaataa gtcttttttt
     2461 agttctgaag gaatatcgga atttatctct gatttttgtg cattattttc tttattttgg
     2521 gtattttgtt ctctttctaa cttttctaac tgttgtaaaa gttccaattt tcgcttttgt
     2581 tctttgattt tttcttgtaa tgaagtaggt gctttttcag attgaacttg ttggttagct
     2641 agttctaatg ctttgttttg ttcttctttt gttggggcat gtttgttaaa gtcgtctgat
     2701 ttttttatct caggtttaaa tgtaaattga tagccaatga caggctttcc ccgtcctttt
     2761 ccgtatttct ttttgattgt taaaccttta aagattgcag ttagttcttg tcgtattgga
     2821 ttgaacacac gttgatcaat cgtaccttgg ttgtaacttt taggaatatc aagtaattca
     2881 tgaaagtctt cttttgaaaa ataagcgaaa cctttggttc tgtattgttt tagtaatcga
     2941 aacattgttt ttgaataact gctttctaat tcattgaatt gttggagaga atagcgcacc
     3001 cattcttcaa gattattaag taagggtaag gctttttcat gaacttgtat ttcagcaaaa
     3061 ggaatatcag attttccgtc gattttaaat tgattaaata aaacaaaacg ttcacgttgc
     3121 aaaccgtcag cacttcttct accaaaacgt aaatccatta atttgtcata cgttgtttct
     3181 aaatcatcta tgaatcgaac atttgcagtt gctttataat tgcttaaatc ttttaattgc
     3241 tcgaatgtaa attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag
     3301 aacaaattca tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga
     3361 tactttacga tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata
     3421 cacgttatga aatacaaaat gcctatttat ctacatctta tattaacatc gtcgatgtac
     3481 taattcaatg tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     3541 cagacaaaac gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg
     3601 gagagtaagg atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata
     3661 aaagaggggg acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg
     3721 cgcatttttc tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc
     3781 tgctctcaca ttatactgcg cagtcgcttc cggctccttt gctaaaaata ttcataagga
     3841 actctttgag aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc
     3901 ccttatcttg ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag
     3961 ttgtctgaat gccctctaaa attcgattta agcactctta aacctattct tatatattta
     4021 tatacttaga cgtttcaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct
     4081 cgtgtgccga cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct
     4141 tcagctttgt catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag
     4201 catatatttt ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca
     4261 ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact
     4321 ttctacaaaa ctcactaaag ggtttggcaa gctctatatt tgctagccaa atacctacgt
     4381 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat cccccctcaa
     4441 aacccacgct ccacaataca aaaaaggcta gcaaatgcta gccacttttt gtaaagtttc
     4501 gcttgtttca ccttcggtga aattgttgct ttgcaacgat caggtagagc tacatttttt
     4561 ttcgttgttc aaatattctt acgccattta ctattaaaat cgtaggtatt ccaaagtaaa
     4621 gaaaaaattg attagcaggt aatacaaaat ataaaataat accagcaata attaccaaac
     4681 ttatagtaag ttttgttaat ttcatgaagt atgccacctt atcttatata gatatcaaat
     4741 agattaatat gccgattgta ttataaaatc catgagcaac tatggaatac catatatttt
     4801 ttgtttgtat ataaacaaaa cttaaaataa taccaataaa gaaaatactt aggcctcccc
     4861 catgaacaat agaaaacaga aaagaagaaa ttagtattga aatatattgt tgttttttgt
     4921 ttgcttgagg atcaatcata caaaatcgaa aaattccttc ttctaaaaaa gctccataag
     4981 tagcagcatt taaaaagaat aaaaggagag aaccatttac ttcattagga tttatacctt
     5041 ctgaaaaaga tgtatttaga atattgatta ggaataaaac agagaatg
//
LOCUS       DANKBF010000125         4997 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000125 DANKBF010000000
VERSION     DANKBF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..182)
                     /locus_tag="PWY89_002848"
     CDS             complement(<1..182)
                     /locus_tag="PWY89_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2067820.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="PWY89_002849"
     CDS             517..951
                     /locus_tag="PWY89_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2067821.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="PWY89_002850"
     CDS             974..1294
                     /locus_tag="PWY89_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067822.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="PWY89_002851"
     CDS             1435..1626
                     /locus_tag="PWY89_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067823.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="PWY89_002852"
     CDS             complement(1697..1954)
                     /locus_tag="PWY89_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL2067824.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="PWY89_002853"
     CDS             2112..2375
                     /locus_tag="PWY89_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067825.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="PWY89_002854"
     CDS             2399..2587
                     /locus_tag="PWY89_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067826.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="PWY89_002855"
     CDS             complement(2670..2855)
                     /locus_tag="PWY89_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067827.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="PWY89_002856"
     CDS             3070..3441
                     /locus_tag="PWY89_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2067828.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="PWY89_002857"
     tRNA            complement(3591..3663)
                     /locus_tag="PWY89_002857"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="PWY89_002858"
     CDS             complement(3709..4137)
                     /locus_tag="PWY89_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067829.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="PWY89_002859"
     CDS             complement(4197..4955)
                     /locus_tag="PWY89_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2067830.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1742 a          784 c          977 g         1494 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaa
//
LOCUS       DANKBF010000126         4933 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000126 DANKBF010000000
VERSION     DANKBF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            85..972
                     /locus_tag="PWY89_002860"
     CDS             85..972
                     /locus_tag="PWY89_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2067831.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="PWY89_002861"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="PWY89_002861"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2067832.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="PWY89_002862"
     CDS             2274..2720
                     /locus_tag="PWY89_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2067833.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="PWY89_002863"
     CDS             2993..4345
                     /locus_tag="PWY89_002863"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL2067834.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="PWY89_002864"
     CDS             4516..4803
                     /locus_tag="PWY89_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067835.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DANKBF010000127         4880 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000127 DANKBF010000000
VERSION     DANKBF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..675
                     /locus_tag="PWY89_002865"
     CDS             <1..675
                     /locus_tag="PWY89_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2067836.1"
                     /translation="IPELFIIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFI
                     TLNDNGQSYADNFTIQGLLEYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMG
                     VGIGNAEFTNFFVSEFYKSFGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKR
                     SFMKDKDASLVKVIAIMSVFFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKN
                     TKGEIL"
     gene            672..1391
                     /locus_tag="PWY89_002866"
     CDS             672..1391
                     /locus_tag="PWY89_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2067837.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1406..2491
                     /locus_tag="PWY89_002867"
     CDS             1406..2491
                     /locus_tag="PWY89_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2067838.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2627..4159
                     /locus_tag="PWY89_002868"
     CDS             2627..4159
                     /locus_tag="PWY89_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2067839.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            4337..4558
                     /locus_tag="PWY89_002869"
     CDS             4337..4558
                     /locus_tag="PWY89_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HDL2067840.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1706 a          518 c          942 g         1714 t
ORIGIN      
        1 attcctgaat tatttataat atttgttgca atagtattca ctttaaaagt tgattttaaa
       61 gtagtgatca agatattatc atgcgtaata cttgtgtcga tagtattatc cttttttata
      121 acaatattta ttacattgaa cgataatgga caaagctatg ctgataattt cacaattcaa
      181 ggtcttttag aatatgcgtc aagagattct ggatatgatg gtgttggtga tttaaatcga
      241 ataactggta tctctagagt agtagatgaa atatttaata ataattttag attagtccta
      301 atgggagtag gcattggcaa tgcagagttt accaattttt ttgtcagtga gttttataaa
      361 tcgtttggta atcttcatta tcagtatttt catcatattt ggttatttat agaagttgga
      421 atattaggag tttcgatatt cctatttttt ataataaaga catttttcaa tgcaaaaaga
      481 tcttttatga aggataaaga tgcaagtctt gttaaagtta ttgcaattat gtcggtattc
      541 ttttttgtgt ataacacaac acttaggggt gaaacaacgg ggccactgat ttatatgatt
      601 ttagccatgc ctgaagttgt gaaaaggaaa gagcattatt atagtgaaaa aaatacaaaa
      661 ggagagatat tatgattcct aagaagatac attactgttg gttcggggga aatccacttc
      721 cgaaaaaaga tagaaagtgc atagaaagct ggaaaaagta ttgccctgat tatgagatta
      781 ttgaatggaa tgaatcaaat tatgatttaa ataagaaccg ttatatggcc gatgcatata
      841 aagaacgtaa atggggtttt gttccagatt atgcaagatt tgatattatt tataatcatg
      901 gtggatttta tttagataca gatgttgagc taattaagtc gttagatata cttcgtgaaa
      961 atcatgccta tatgggattt gaaggggaaa tctgggttaa tggcggtatt ggttttggtg
     1021 cagaagaagg acattctgtt attaaaggtt taagggatat ttacgattca ttaagttttt
     1081 atcaaaatga tggtacattg aacttgaaac cgagtccgta ttatattaca gattatcttg
     1141 aatctattgg attggttcgc gataacagcg aacaaataat aaatggcctg catatctatc
     1201 ctacggatta ttttgctgcc aaggattatg atactggaaa aatttataaa actagtaata
     1261 caataagcat tcatcaatat aatgcatctt ggaaaacacc taaacaaaaa tttgtttata
     1321 gattaaaatc aacaattgga caaaaaaaat ttaacaggtt acttgcactg aaaaaatttt
     1381 tacacttata aaggagaaaa gtactttgaa gaaaatttgt ttcattagtg gtgtaatttc
     1441 tcgttctggg ggaactgaaa gagtagggag tattattgcc aatagcttgt ctgatttagg
     1501 ttatgatgtc actattttaa gcttttggaa tagtggagag ccttattttg ggttagataa
     1561 gacgattaag gttgattata tgttaaatcc taaaacggag ggaaaattat atagaactta
     1621 tttatatccg gtttggaaat tgcacaagta tatatgcaaa aatgaatttg atatagtaat
     1681 tgatattgat actttgttgt ctttgtatac tacaagggca attaaaggaa caaagtgcaa
     1741 attgatttct tgggaacatt ttaattactg gacaatggaa atgctgaaag aaaagaagag
     1801 attcaaggct aaaaaattga taaaaaaata tgcaaagagg cttgtggttc tcacagaaga
     1861 agatcgtcaa aaacatattg atgaatatag tttatcagaa gattttgtta taactatgcc
     1921 aaatccgtgt atatctaata tcgagtacga atatcattat gataaaaaga gatttcttgc
     1981 agtagggcga ttagtggaag agaaaaattt tgctgacatg ataagtgctt ggaaaattgt
     2041 agaaaaaaaa gtagatgatt gggatcttat cattgtagga aaaggtgaat tagaaaaaga
     2101 tcttcgccag cagataaaag atttaaatct gcaaagagta actatagcag ggcattcaga
     2161 ccatgtggag gaatattata aatctgcatc ttgttatgtg cttagttctg tttatgaagg
     2221 atttcctatg gttatattag aagcacagtc ttatggatta ccagttattt cttatgattg
     2281 taaaactggt ccgagagatt tggtacatca taattttaat gggatgttgg ttgaagataa
     2341 gaatatagac caattagcta agagcatgat aatgtttact aagaatactg atttagcaat
     2401 gagaatgtca ctgaacgcat ataataatgt acaaaaattt aatttgaaag aaattacaaa
     2461 acaatgggtt gctttgattg aaaatgtata gttttttgtg gttgtccaaa catgaaaatt
     2521 agcgtaatta atcaaagagc tagaagaaac ctattacatt tcaagatttt agaaattata
     2581 tagtattagg taacaataaa aaatagaaag tttggagcca tatgatatga taattaatcg
     2641 taaacaaaat gctaaaagga atataattac tggaacaatt tttaagttat atcagattat
     2701 cattcccttt tttatgcgta cacttatgat ttatcagatg ggaatggagt atgctggatt
     2761 gaacaacctg ttcacatcta tatttcaaat gttaaattta gcggaattgg gaataggtgc
     2821 tgcactaact gtaagtatgt ataaacctat agcagaagac aatagaaaag agatttgtgc
     2881 tttactgaac gcatataaaa agtacttttc aattattgga tggatagtgt ttacattagg
     2941 attgttatgc attccttttt tgagattttt agttaaaggt tcgattcctg gaaatttaaa
     3001 tatgacagtt ttgtatttaa tgtatttgat aaatactgtt ttgtcgtatt ggttattttc
     3061 atatagaaag agtctaatat atgcacatca aagaaatgat gtaattaacg tgtctatgct
     3121 aattactttt acattccaat ttgtaatgca ggcatatgtt ctgattttct taaaaaatta
     3181 ctatttgtat ttattggctt ctattttggg acaaatagtt cttaacttaa tgtgtgctgt
     3241 tgtttctcaa aagatgtatc ctgattatac tcctgttggt gagttagaga aagaaactat
     3301 tgacgatatt ataaaaaaag taaaaggatt agttacaaac aaggtaggcg gtacaatatt
     3361 acgatcttca gattcaatta ttatttcatc tttcttaggt ctttcagtac ttgcgattta
     3421 tcagaattat tactttatta tttctgccat aatgagtata tttgcaataa tttatgaatc
     3481 atgtatagca ggtataggta acagtcttat tgtagaaagt caagaaaaaa attatataga
     3541 tttttgtgat attacaacaa ttataggttg gtttctggca attagttgtg catgctttat
     3601 tacattattt cagccgttca tgattatctg ggtgggaagt gataatctta tgagtatttc
     3661 atttgttata tttatgatag ttcatttttt cttatatgaa attgatcagt taattgggtt
     3721 atacaaagat gctgctggaa tatggtacgc agacaggttt agaccattaa ttacagcaat
     3781 gataaacatt gtattaaata tcatcctcgt taattggttc ggattatatg gagttttggc
     3841 ggcaaccatt gtttcagtat tgttggttga tttaccctgg ttattatata atgttttcca
     3901 aaatatattt aaatttgaat cggcaggtaa atatgtttgg aagctcataa gtttgtgtgt
     3961 aatagctttt gttgcttctt atttaagtta ttttgcttgt tcgtatgtta atatcggaaa
     4021 tttcaaagtg ataagtttag tgataaattt agttatatcg gttgtcatat cgattttagt
     4081 tattcttctg tttaatatcc gtaataagaa gtttaagtat attttgaaag ttcttacgtc
     4141 ggtattggta aaaagatgag aagagacatc agaatgatat ataaagtaac tacaattttt
     4201 aggctgccga ataggcgaac gatgtatagt ttttgttcca acgaagactc ttgttgaaat
     4261 tggagatgat atgaaggagt tacaattttg actcatgttt atgattgatc ggttttaaaa
     4321 gtagtatgga gaagctttgg gatcatcgga gaggatcaaa ataggtaata atgtttttat
     4381 aggaatgaag tctagaatac taaagggtgt acatatagga aataatgtag ttataggtgc
     4441 aaatagttta gtaaacaagg acgtatcaga cgattgtgtt gttgcaggaa atctagcaaa
     4501 agtaattatg tccctagatg aatattataa aaagataaac aatatgaaga atccgtaaaa
     4561 cttgtgaaac tataccgaga ataatatggg aaaaatattg atgaaaaagc attatatgag
     4621 tttttagggc tgtttacaga tggagatgac aatttgtcga agagttggga gcaatgcaaa
     4681 atttagtagg ggattataaa cagactgaca aggtatttaa agaacatgca caagtatttc
     4741 ataatattga agaatttttg aatactgtga gataggagtt gctacattga gaaaagtatg
     4801 tatttttcca atgtaaaaga ttacgagttt gaaaacgaca ccataatatt attttttaag
     4861 aagatattat agagcttagg
//
LOCUS       DANKBF010000128         4871 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000128 DANKBF010000000
VERSION     DANKBF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="PWY89_002870"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="PWY89_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL2067841.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="PWY89_002871"
     CDS             724..1878
                     /locus_tag="PWY89_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2067842.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="PWY89_002872"
     CDS             2093..3061
                     /locus_tag="PWY89_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL2067843.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="PWY89_002873"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="PWY89_002873"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL2067844.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="PWY89_002874"
     CDS             4091..4699
                     /locus_tag="PWY89_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL2067845.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(4779..>4871)
                     /locus_tag="PWY89_002875"
     CDS             complement(4779..>4871)
                     /locus_tag="PWY89_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2067846.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1501 a          936 c         1135 g         1299 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaaatc ccttacggta tctatgattt aagctgggat tcccaataat
     4801 accttgattt cagtacagac cgaaaacccg aagagagtgc cttcttttcg ggttttctta
     4861 tataatcctc g
//
LOCUS       DANKBF010000129         4794 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000129 DANKBF010000000
VERSION     DANKBF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..701)
                     /locus_tag="PWY89_002876"
     CDS             complement(<1..701)
                     /locus_tag="PWY89_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2067847.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTH"
     gene            complement(1269..2336)
                     /locus_tag="PWY89_002877"
     CDS             complement(1269..2336)
                     /locus_tag="PWY89_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2067848.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2356..2835)
                     /locus_tag="PWY89_002878"
     CDS             complement(2356..2835)
                     /locus_tag="PWY89_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HDL2067849.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2852..3313)
                     /locus_tag="PWY89_002879"
     CDS             complement(2852..3313)
                     /locus_tag="PWY89_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2067850.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3327..4577)
                     /locus_tag="PWY89_002880"
     CDS             complement(3327..4577)
                     /locus_tag="PWY89_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDL2067851.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT         1515 a          977 c          602 g         1700 t
ORIGIN      
        1 ttatgtgtca atgaattatt attattacat ctataataat agatacaatc tttaataaaa
       61 cttgttattt ttgaatgttg caaatatgaa atatggaata tgatatcttc agatatataa
      121 tccctttctg acggaaactt gatattgtgc tgttgaatta tctcagatga atacagcgca
      181 tgccaaactg acattggcaa tataaaatcg actttataac tcggctcagt gccaatcatt
      241 ccaagcaaca ctttttcaat tatagcgtta tttgtaaata tcgtactatc atatcttgct
      301 ggctttgaaa taatattatt atcatcataa tattcataat atccagcata acatgtgtct
      361 gcattactcc tttttatacc atttaacatt gcttcaatat aattagctgc aacaaaatca
      421 tcagaatcaa taaaagcaac atattttcct tttacatgtt caagtcctgt atttcttgcc
      481 aatcccaagc cagcattatt tttatgaata atacgaatcc ttgaatcctt taatgcatag
      541 tcttcacata ttttattaga tgaatcctta gatccatcat ccacaattat aatatctagg
      601 ttcttatatg tttgattaca cactgattcc aaacaacttc ctacatactt ttcaacatta
      661 tatacaggta tgattacact aattagatca ctcgaattca tccttctatc ctttcactca
      721 cgttttctca attagtcaat gacggcatca acgattttct taaattctct gaaaaaaata
      781 gactctgatc ttgatgatgt gaatgacata ctatcttctt ctagaaaaat tatttccttt
      841 aaattattta ttaaatcaat aatatgatcc tcgtaagtat gctttccctt ggaataatga
      901 aaatatactt ttgtattaca caatattgca attttatttc ttaatatgat tatttcactc
      961 attcattact gccacatata cacaacttat aaccaccccc tgcaaagttt agggtaagtt
     1021 atcagataat atcttcctct tcatcatcca gaatacaaaa tctatttatt tatagaaata
     1081 tcattaatgt ttttatataa tgtaccgtgc ttgtactctt gctctattac aagcactaaa
     1141 acactactat tcatttgaat tcttacacgg ataaaaattt acatcgtcta tttcaatatt
     1201 ttttctctct attttattca cttaataagt tcattacata atcgaaacat tttttatttt
     1261 ccttgtcatc actccttgct ccctttttga tacagattta aaattctatc aaagaaattt
     1321 ccatcgttaa atttcgttat tgccaaatcc ttcgaacaac gtgaaaattt tacgtattta
     1381 tctttatcat tgatttttag tattgcagat gtcaacgctg atacgtcacc aggttgaaac
     1441 aaatatccat tttctccatc acgtactgct gaagaaatat ctcctacatc tgtagcaatt
     1501 acaggcattc cataagaaat cgcctctaga atagatatag gcaatccctc gttataggac
     1561 ggtaaaatca tcacatttga tcttctatag cactcctcct ttttttctcc cacaacccaa
     1621 cctgtaaatt caaaatacga ttgcaaatta tattcttcta tcaattcttt gagcgatatt
     1681 tcttcatcac ctgctccagc aatctcaaaa ataaactttt tttctggata ttgttctgca
     1741 atgtttttaa ctgcttctac caaatcgtgt acaccttttc gctttatcaa aactcccata
     1801 aacagaaatc ggcacatttt tgaattccac tttatggtat attctggaac tacaatagaa
     1861 ttttctacaa caacagtttt acaatttggc tcaatttctt taattatctt cttccatttt
     1921 tctccaagta caatcgtgga atcagactct cttaacagca agcgaatctg attttgtttt
     1981 tttacagaaa cactatcata ccatttttta aattccgatc catgaagatg gatgattaca
     2041 ggaatattta ataatctaaa ccacttattc agcacatatt tcctactaaa actccctctg
     2101 tacgacatgt gcatatgaat aatattaggt ttactcaata aagagatacc tataatttta
     2161 gcaaatgcat acataaaata taaagattta aatattgcat ttccaccctt ataactcggt
     2221 ataaaagtca tctcgatatt ctttttttcc caattaaaat tgagcatttg tgtaattata
     2281 cttgtaattc caccttttac cgagctgtca ttaccaatca taagtactct ttgcataacg
     2341 ctttactcct tatatttata ttttttctat taatacctct atcgaattaa taatcgtttc
     2401 cgtgttagat acatacttgt taaacatccg aactcttgtt tctttaacgg catcttctaa
     2461 attttctaat tcataacaag gatagataat attcatctca tcaaattgtc taattaactg
     2521 cagttggtga tcatcgacat gctcaccata cttagccaat cgaggaacag caatgaccct
     2581 tttacctttt tttacggccc caattatagc acctgtacct ccatgagtaa tcaccatttc
     2641 tgctttttgc atagtttcag taaactcatc cctatttaga aatgctttat aggaataatt
     2701 tagtggttta taatcactat aaccactctg tgcaaatgta ctttcgatta ttactccttt
     2761 ttcaattaaa tcatcaatag cttttaacaa tctatcaaat tgaaattttt gtgaacctaa
     2821 tgttataaat atcatttttt tctcctatta tttaatatat cccacccaaa taaatagcat
     2881 taggataaaa ttgtagcata gtttcccact gaacataaaa ttgatctgca aaatgataca
     2941 tcaattttcc tgtctgagta gggctggtta cttttgcaaa cgattcaata taaatcagtt
     3001 tttttcccat catttttgtt attaagcaga tagggatcat agctaaaaca cctgtacaaa
     3061 tcacgagatc cggtttttcc tttatatata taataattga tttgaataaa ttaataagta
     3121 tagtacacaa aaaaaacttt tcttttctat ttacttgtct catgtagtac gttttttcgc
     3181 cttttgactc agctgaatat tctgtctgtt ctgttataac aaaactctca tacttctcca
     3241 tcagtggttt taacatcatt aactgttcat aatgcccccc agaggaagcc gcaaaacata
     3301 tctttttgtt catattaacc tctctatcag tcacgctgaa aaatatctct agtgtaaact
     3361 ttttctttaa tatcatccaa gcaggtatca tatcgattgg caataataga atcacacatc
     3421 gccttaaact gctctaaatc gttgataacc ttacttccga aaaaagtctc tccatctttc
     3481 agtgcaggct cataaatcac cacagtcgca cctttagctt ttacgcgctt cataactccc
     3541 tggattgaac tctgacgaaa attatcactg ttgctcttca tggtcagccg gtatataccg
     3601 attaaaactg gattttccct gttgacatca tactgattat attcagcgta gttgtaatag
     3661 cccgccatct gaagaacacg atctgcgatg aaatccttac gggtacgatt agattcaacg
     3721 atagcctcaa tcaaatttcc aggaacatct gcatagtttg ccaacaactg cttggtatct
     3781 tttggcaagc aataacctcc atatccaaag gatggattgt tgtagtgagt tccaatacga
     3841 ggatcgagac aaacaccatt gattatctgc tgagtattca gccccttcat ttcagcataa
     3901 gtgtctaatt cattgaaata gctgactcgc agtgctaaat aggtatttgc aaacagcttt
     3961 actgcttcgg cttcagtgaa tcccataaaa agagtatcca cgttctcctt aattgcactt
     4021 ttctgcagaa gtgttgcaaa aacatgagca gcatttaaca agcgctcatc agtcatatca
     4081 gtaccaacaa taatacgaga tggatacaga ttatcatata aagccttgct ctcgcgcaga
     4141 aactctggac taaaaataat gttcttactc cccgttgtct cacggactga ttcagtgtag
     4201 ccaacaggaa ttgtagactt gatgaccatg atggtatcag gattcacgtc catcacaacc
     4261 tcaataactg actcaacagc agaagtatcg aaatgctgag tacgactgtc atagttagtc
     4321 ggtgtagcaa taacaacgaa atctgcggat ttataagcgc tccttgcatc caaagtagca
     4381 gttaagtcaa gttctttttc tgtaagatac ttttcaatat attcatcctg aattggagac
     4441 ttccgtttat taatcagttc aatcttttca gggataatgt ctaccgcggt tacctggtga
     4501 tgctgcgcca atagtgtggc gatactcaaa ccaacataac cggtgccggc tactgcgatg
     4561 ttgtactttt tcttcacaat atctcctctt tcctctctta agtatggaga atgttactcc
     4621 tacggctatt catcatgtca aaataacaca gcttagaatg ttgataaaat aattttccag
     4681 aatgtgataa aatctctttc atttataaac cacacatcta attttatcta ttctccacaa
     4741 gacatgtaga gtattcttta ataacaagat acgaccttac taaatacttc aaaa
//
LOCUS       DANKBF010000130         4622 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000130 DANKBF010000000
VERSION     DANKBF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            136..954
                     /locus_tag="PWY89_002881"
     CDS             136..954
                     /locus_tag="PWY89_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2067852.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            958..1254
                     /locus_tag="PWY89_002882"
     CDS             958..1254
                     /locus_tag="PWY89_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067853.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(1436..1789)
                     /locus_tag="PWY89_002883"
     CDS             complement(1436..1789)
                     /locus_tag="PWY89_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067854.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(1809..2864)
                     /locus_tag="PWY89_002884"
     CDS             complement(1809..2864)
                     /locus_tag="PWY89_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDL2067855.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            3080..3478
                     /locus_tag="PWY89_002885"
     CDS             3080..3478
                     /locus_tag="PWY89_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067856.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            3481..3654
                     /locus_tag="PWY89_002886"
     CDS             3481..3654
                     /locus_tag="PWY89_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067857.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         1602 a          641 c          791 g         1588 t
ORIGIN      
        1 tatacatata tatgtttatg ttgtagaata taaaagcaaa tataaatata aacacgttta
       61 tgtatataaa catatacata aacgtgttta tagtgtataa tggagttaca aaaggttaat
      121 aagggaggtt taatcatgga aaagaagttg tcaatcatta ctggaaacat tgaaaaaggt
      181 ggaacaggta aaacaacaac aatctacaat ctaggtggat atgctgtcga aaatttaggt
      241 atgaaggtgc tgtatattga tgaagataag tcgcaaaacc tatcaaaacg atttcctgat
      301 gctttcagta aagcgaaaag agagaataca atcaatcatt tatatcttca tggatcagct
      361 gaacctttag ctataaatga gaacatggat attttagtag caggtgaaga tttaaaacaa
      421 gttgagatgc aaatgcgtga tatgcctaac aacagaaccc tactgtttag atggatcgtt
      481 gttaactatg aacagcttaa agagaagtat gatctaattc tgattgatac tcataatgat
      541 gcaggtctac tagctcaaaa tgcttgggct gtatctgatc ttgtcgtagg catttctgat
      601 ccatctaaag atggttttga agctttgctg aagcttggta aagatattga tgcactagca
      661 aaacagttga ttgatattaa gactggtgaa acactgatgg aaactaaata cgctacgatc
      721 ggtagcagga tcaaacataa tactaattca agtcgtgagt tcctagaatt ggtaccaacg
      781 ttatctaatt atcttggata tgtacaagag aaggaattgc ttaatgcaac tagcttagaa
      841 ggtaagaata ttgctgagtt cgctaaggat aagaatatct atcgtaagaa taaggatttc
      901 tttgaaagca cgtttacgct atacaacaaa ataattgata cggcacaaga ataaggagtg
      961 attgaagtga aagaaaatag atttaaaaaa ttcgctgaag ctgaagtaga agaagttcct
     1021 gaagtggttg aagcagttga agaacctaaa caaacaccta aagaaacaac aagttctttg
     1081 gtagctaaac taaaaagtga tagttctgaa aagaagaaaa attatcaatt cacgcttcaa
     1141 ccatcacaaa gaaagaaatt atctaaaatc agcgaaaagt taggaattcg atcagattca
     1201 gctttacttg gtgaaatgat agataacttg tatgatgaag tttttggtga gtgatcagtt
     1261 taaagctgat cacttttttt attggaataa gcggttaggt atagcttaac taaatttcgt
     1321 atacgtgttt atggatatat acgtatacat gtttatatac atgtatacgt atatgtttat
     1381 gtttgtattt gtacataata aaaaggctag gtattgtaac ctcgcctttt tatttttaaa
     1441 gtaagtcttt gttacttggt gcattgaacc cttttagcat tggcttttct ttctttgctg
     1501 aatgatcgga tgtaaccgtt gaaggatgct cttgttgaat agggttatga tcagttggtt
     1561 tcttctttac ttctgctact ttgctatcgt ttctatcgtt gcctgcttca ataattgtaa
     1621 cttcattgtt ctttacacgc cgatataggt tcaaagcttt tttgatcgtc tgagagtatt
     1681 taacgcctag tgtttcatat tcggattcaa gaaactctgc taaaggtaac tcgctaacct
     1741 tttctctatt gattataact acataacgct ctgattttcc cttattcatg ttgatctcct
     1801 tcgctgtttt attgagttaa accaaacttg taaaagcctt ttacattaga aaattcactg
     1861 tcttcaacaa acaaggtatg cggaatttta tcttttacaa gttgtttgtc gataatagaa
     1921 gctccaccac cagtaaagac aactttatct aaggttgtga agtttttgaa tgtagctaaa
     1981 acttctgcaa caaccatatt ggtgtatgtt tttatttctc tttcaactac ttctgaaata
     2041 tcaaaacttt catttccgct tggcttaaat agatatacgc ctttttcttt gccttctcgg
     2101 ataatctttt caagttggaa agcattagga ctgatcggaa ggtcaagcat atttacgata
     2161 cgattatata aagtaaaaat gccggtatcc ttttgttcac gattgttttc gtcaatttca
     2221 aaattttgga caacatcaaa aagtttcgtg ccaccaccaa tatcaacaaa accgatcttc
     2281 tgactgatca atgtttcatc ttgtacacta ccatcatcag caagtaggag gttatatagt
     2341 gttccaatcg gctgtggcag tacccaaacg gtatctacgt ttatattata agccttaccg
     2401 tcaacttcaa ctgaatgttg tcgtgtggct tgttgcatca cgtatttgac gattgtttcg
     2461 gtatagtcgg atgaaggcag acctaaaact agattaacgg ttaaaacgtc atcagattga
     2521 tcttctaaac gtgctaggtt agctaaagag aaagatagga tatttttgta ggtttcctgc
     2581 ttgtatctgc tgatcccaaa acctagtgat tctatcacgt agtcatcttt tcctagctcg
     2641 tgtatatcct tcccaaaata atagctttca gtagtgttta ctaggctata ttcagaaacg
     2701 tttagtgatc gttttaatga ggaacctata ttagaaaaac gtcctgaatt aatgatattt
     2761 ttgttcaaaa tacgagaggg aagtgagagt ggagaaacgt gtgtactcat ttcttttact
     2821 gatttattcc ctaagtcaac ggaaaacaat aatggttctt tcatggtatg acctctttct
     2881 gtttgtgttt atggtatttc ggtacgaaaa tagtttacac ctatttacta ttagttgcaa
     2941 ctgtttataa gaaaaaaatg caatcataaa agtctaatta attataataa agtgtaaata
     3001 aaaaggtgta aataaatgct tgcaaattta tcttattatg atattataaa atcataaatt
     3061 atattaagga gtgatcctga tgcgattgag aagaaataag aattacacta ccattatttt
     3121 agaagatttt actgatcaag aattacttat tgtcggtaaa acaaaagcag attttatcga
     3181 aaagggaaat gcttcaatag taaaagcttc aaaggttgct gtcgaaaaat attttgattc
     3241 tttagaagaa agtgctaagg aaatttttaa tggagagaaa gctggtagct atgagttaga
     3301 cggtattaga tttagcttga tcccaacaac tgaaaatatt attccgaaga aattatatta
     3361 tatcttttca tctgaaaaac atacttcaat gaagccttgt atttttgatt ggcaatggtt
     3421 gattgatgct gtattggatg taaaatgttc aaaatacaat attactttgg ggtgttaatt
     3481 atgagtatgc tagttagaaa attaccatta tctgtaagag aatttctatt ttacttggtc
     3541 tgcttaatcg catcagggtt taagctgatc gaacatattt ttatctttgc ttatgtatta
     3601 gcaatgatcg catttttatc tggcggttgg ttaattattt atttgttaag ctaagtttta
     3661 gatcaaaaat aattagtaaa ttaaacgata aaataaccgt taataaatgt aatgaaagga
     3721 caacgcctaa caatcagctt gtaataagat tataaaataa cataatatca aaaatagtgc
     3781 tttttaaagg acaaattaag tgtatcaaca taaacgtgta tatgtatatg tttatgttga
     3841 tacttttttt gtcttaattt tttattttta aaattctatt tactaatgct gtatagatag
     3901 tgatcagaaa aaacaaaata gcgagattaa gctatttatt agttcatata ttatattcag
     3961 attaatatga tccagtatta atctgaaggc aaaaccaata tatttctacg gactcttgta
     4021 aaactattta aattttcctg ctaataaaat aacgaaaata gaatatttag cttttttata
     4081 ccattttacg ataacatgat ttcatttgta atattaaaac atttaaattt ttaattattc
     4141 tttcaaatta gatcaattaa tatccttata aaagagcata ataaatggtt tttacatcgt
     4201 tatttattga tattaatctc atccaaaaaa aatgaaaacg catactttta tgggtgattg
     4261 aatgtgattt taatacatat accaattaaa agaaaacttt ttagattttt acttacaaat
     4321 ttataaaaaa acaagataat tgatactttg attttgcgaa cattttatct tgccgcctaa
     4381 ttcagcttta atatcatgat tatatcagta acaaatcgct tttttagcta atttaagcat
     4441 caaataatta ttcttaatca gggaaaaaat aactaataaa tggattttgg gaataggtaa
     4501 aaacttgatc ggtttctggc taaaaataac gtgaaaaagg aaatataaaa taacatggag
     4561 cgtgggagga ataaagaaat tttacacgaa aggtgcgcag ggatgtaaag aaacttaaca
     4621 cg
//
LOCUS       DANKBF010000131         4558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000131 DANKBF010000000
VERSION     DANKBF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..790
                     /locus_tag="PWY89_002887"
     CDS             <1..790
                     /locus_tag="PWY89_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2067858.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            887..3136
                     /locus_tag="PWY89_002888"
     CDS             887..3136
                     /locus_tag="PWY89_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2067859.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3139..4314
                     /locus_tag="PWY89_002889"
     CDS             3139..4314
                     /locus_tag="PWY89_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2067860.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4317..>4558)
                     /locus_tag="PWY89_002890"
     CDS             complement(4317..>4558)
                     /locus_tag="PWY89_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2067861.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1578 a          659 c          834 g         1487 t
ORIGIN      
        1 ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc gagaacaata
       61 cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc aaaaatattt
      121 tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt atcaagcaga
      181 tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg tagaatacta
      241 tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa atttaaattc
      301 atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag tcagtgaatt
      361 gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag ctagttatct
      421 gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa ttgaaataat
      481 acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc tatcgataca
      541 cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa aagagtatat
      601 tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat attcaatcca
      661 gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag cctttaagaa
      721 agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag gaatcattaa
      781 tggtgaataa actaaacatt tgattttgat aaatattcgt tactttaaac gcttactatt
      841 tatgtgagcg ttttttataa tgcaataaag aaacggagga gagcttatgt ataaggataa
      901 tgaaatgaat attatttatc gatcagaaaa ccaacaattt catttgttta atcaggagat
      961 aagttacatt tttaagatat cggaagatgg taaattattg catttatatt atggtgagaa
     1021 cttaccagat aatgactata gtcatctaat tgagatgcat catcggccta tgacaaccta
     1081 tagaaatgaa aacgatttgc tatattcgtt agagcactta aaacaagaat ttcctgaata
     1141 tggtacaaca gattttagac atcctgctat ttcattacta cagaaaaatg gctctcgctt
     1201 atcagatttt gtctatcaag ggtacgaagt ggaagaagga aaacctaaac tagaaggttt
     1261 acctgctacc tatgttgata ataatgatga aagcaaaaca ttgaaagttt atattttaga
     1321 ttcagttaca aatgttcaag tagaattatt atatacaatt tatagagatt atcctgttat
     1381 tactcgtagc gcgcgagtta tgaataaagg aaaagatatt caaataatcg agaatattag
     1441 cagtttatca ttggatttac ccgatgcaaa ttatgaatgg caacaattat ctggtgcttg
     1501 gggtagagaa agaactatta agtcgcgtac tttacaacaa ggaattcaat caatcgaatc
     1561 tactcgaggt atttcaagcc atcagcataa cccatttgtt actttaaaaa gaaaaaatac
     1621 tgatgaaaat caaggagaag ctattggtgc agcactagtt tactcaggaa acttcttaat
     1681 tcaagcagaa gtagatacat gggatgttac tagactacaa gttggcatca atccatttgg
     1741 atttaattgg aaactcaatc caggagaaat atttacagca ccagaagcga ttctggttta
     1801 ttcaagtaag ggtttaaatg ccatgagtca aacttttcat caactattta gaaaacgctt
     1861 agctcgtggt aagtggcgag aaaaagaccg tcctgtctta attaataatt gggaagcaac
     1921 atattttgat tttgacgaag aaaagttggt aaatattgct aaaaaagcat ttgatgttgg
     1981 tattgaatta tttgtattag atgatggctg gtttggagaa agagaaaacg attgtgcagg
     2041 attaggagat tggcatgtaa atcctaaaag actacctaga ggaatcggca gcctttcaga
     2101 aaaaattcgt agttttggta tgaaatttgg tttatggttt gaacctgaaa tggtgaataa
     2161 ggatagtgag ttatatcgca cacatccgaa ttggattatt tctacaccta atcgaaaacc
     2221 taatcatggt aggaaccaat atgtattaaa ttttgggtta gatgaggttg ttgaaaatat
     2281 tttcgatcaa atgtgtaaaa tcattgatga atcaaactta gattacatta agtgggatat
     2341 gaatcgtcca ttgactgatg tatttgacag tcacttgtca gcagatcaac aaggagaagt
     2401 attccacaga tatgtactag gcgtatatcg cttatatgaa aaacttataa caaaatatcc
     2461 taacatacta tttgagtctt gttcatcagg tggtgggcgc tttgatcctg gaatgcttca
     2521 ctatgcacct caagcttggg ctagtgatga ttcagatgca attgaaaggt taaaaattca
     2581 atatggaact agtatgctgt atcctctttc gtctattggt gctcatgtat caattgttcc
     2641 aaatcatcaa acaaaccgta taacacctat aaaaactaga ggaaatgtag ctttttttgg
     2701 tgcatttgga tatgagcttg acttaaatga attaagtgaa ggcgatttga ggattgtaaa
     2761 agaacaaatt gccttttata aaaaatatag aaccataatt cataatggta ctttttatag
     2821 actattatca ccattcgatg atgataatca aaccgcgtgg atggttgtta gtcatgataa
     2881 gaaaacggct attgttgctc attataaaac attaaatgag gtaaatgctc cttaccgccg
     2941 actgaaatta aaagggttag ataatgacgg actttataat gtaggacaaa atagtttgcg
     3001 atctggaatg gaattgatga gagcaggact agtctgttca gatgcatcta gtggacaaat
     3061 aaatgatcag aatcgacgcc cagaaacatt tgattttgat tccaagattt gggttataac
     3121 acaggaggaa aagtagagat gaataaccaa aaaagcttac ttcaaaaagg agcaatctta
     3181 agtatttcac taatgttaac aagctctcaa gcaattaatg gtgttattcc acaaatgaaa
     3241 cagtctttgg ggatatcaca aagtcaaagt gaacttttag gaactgcacc aagtataact
     3301 gttattttgt ttatcttatt atctagttac tttgccaaaa gaataggaat gaaaaagaca
     3361 ataattttag gtttattatt agctggtata ggaggaatta ttccagtatt tacagctacc
     3421 ttccaaaccg ttcttattag tcgagtaata ttaggtgccg gtttaggagt atataattca
     3481 ttggctgtga catatatcag ctctttatac tctggcaata ctagagcaac gctattaggt
     3541 attagaaatt caatggaagc tttaggtcag acaatcctta tttttcttgc tggagtttta
     3601 gtgaacatta attggacggc ttcgttttta gtctatgcaa ttgcgtttcc tgtagctctc
     3661 ttattcgcgc taaaagttcc tgaaattaga gatgaaaata gtgacatacc tgaaaatcat
     3721 gtaaaagaaa agatgaatcc aatggttttt gccctagttc tatttgcaat cttactcgta
     3781 atgaatagta ttgctatttc tgtacgattt gcatcaattg caactgaaat taaaggggta
     3841 aactttaatg ctagtaacta tttagctcta atgcctatct taggaatact agcaggattt
     3901 atgtttggac caataaataa gtggatagga aaaggaaccc tatacttggg aattatattc
     3961 tttataattt caaatttgtt aattgctgtg tcaaacggta atatgacgtt cctgctaacg
     4021 ggtttatttt tatcaagtat aacaggatca tggtgcttcc catttatctt tagtaactta
     4081 gataaagtca ctacaaagaa tacaattaat tttgctactt cattgatttt tattggctgt
     4141 aatattggta attttatagc tcctattgct atgcaattag cacaatttct aacaagaagt
     4201 acaaatttga ctgctccatt ttttgtattc gcaggaatat ttattgtgat cctatttgta
     4261 acattttttg cgacaaataa aaaaaatact agagtaaccc gtggtgctag ttaattttaa
     4321 tatcctaaag aatatctcaa agttccttca aaacgtcgtt gtgctcgact taatagaaca
     4381 ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc ctttaacaga acgtgagttg
     4441 aaattttgac aacctaattt ttctaaggaa gaaaaaacag tctcaactgt ttttctttgt
     4501 tttccaagta agctgtggtc tagtttttct gatttatcca tgtttttacg tggcggaa
//
LOCUS       DANKBF010000132         4500 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000132 DANKBF010000000
VERSION     DANKBF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="PWY89_002891"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="PWY89_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL2067862.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
     gene            complement(3004..3438)
                     /locus_tag="PWY89_002892"
     CDS             complement(3004..3438)
                     /locus_tag="PWY89_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067863.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3455..>4500)
                     /locus_tag="PWY89_002893"
     CDS             complement(3455..>4500)
                     /locus_tag="PWY89_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL2067864.1"
                     /translation="KTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLK
                     MNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTEN
                     LSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSV
                     SGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRD
                     SGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFK
                     NEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQ
                     YQLGLVDNFNNIS"
BASE COUNT         1386 a          842 c          620 g         1652 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2581 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2641 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2701 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2761 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2821 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2881 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2941 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     3001 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3061 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3121 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3181 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3241 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3301 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3361 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3421 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3481 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3541 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3601 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3661 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3721 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3781 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3841 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3901 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3961 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     4021 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4081 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4141 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4201 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4261 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4321 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4381 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4441 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
//
LOCUS       DANKBF010000133         4461 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000133 DANKBF010000000
VERSION     DANKBF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            574..1008
                     /locus_tag="PWY89_002894"
     CDS             574..1008
                     /locus_tag="PWY89_002894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067865.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="PWY89_002895"
     CDS             2298..3221
                     /locus_tag="PWY89_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2067866.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="PWY89_002896"
     CDS             3237..3776
                     /locus_tag="PWY89_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2067867.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DANKBF010000134         4434 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000134 DANKBF010000000
VERSION     DANKBF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(543..2048)
                     /locus_tag="PWY89_002897"
     CDS             complement(543..2048)
                     /locus_tag="PWY89_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2067868.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="PWY89_002898"
     CDS             complement(2045..2731)
                     /locus_tag="PWY89_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2067869.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PWY89_002899"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PWY89_002899"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL2067870.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DANKBF010000135         4425 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000135 DANKBF010000000
VERSION     DANKBF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            690..1658
                     /locus_tag="PWY89_002900"
     CDS             690..1658
                     /locus_tag="PWY89_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2067871.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="PWY89_002901"
     CDS             1669..2484
                     /locus_tag="PWY89_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL2067872.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="PWY89_002902"
     CDS             2552..3262
                     /locus_tag="PWY89_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2067873.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="PWY89_002903"
     CDS             3298..>4425
                     /locus_tag="PWY89_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2067874.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DANKBF010000136         4282 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000136 DANKBF010000000
VERSION     DANKBF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(37..1257)
                     /locus_tag="PWY89_002904"
     CDS             complement(37..1257)
                     /locus_tag="PWY89_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL2067875.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="PWY89_002905"
     CDS             complement(1277..1888)
                     /locus_tag="PWY89_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2067876.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="PWY89_002906"
     CDS             complement(1931..2863)
                     /locus_tag="PWY89_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2067877.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="PWY89_002907"
     CDS             complement(3284..4144)
                     /locus_tag="PWY89_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067878.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DANKBF010000137         4041 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000137 DANKBF010000000
VERSION     DANKBF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            179..775
                     /locus_tag="PWY89_002908"
     CDS             179..775
                     /locus_tag="PWY89_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2067879.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="PWY89_002909"
     CDS             899..2698
                     /locus_tag="PWY89_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2067880.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="PWY89_002910"
     CDS             complement(2976..3188)
                     /locus_tag="PWY89_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067881.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="PWY89_002911"
     CDS             3650..3799
                     /locus_tag="PWY89_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067882.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1189 a          689 c          711 g         1452 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gagatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DANKBF010000138         3894 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000138 DANKBF010000000
VERSION     DANKBF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            287..1102
                     /locus_tag="PWY89_002912"
     CDS             287..1102
                     /locus_tag="PWY89_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067883.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1228..1485
                     /locus_tag="PWY89_002913"
     CDS             1228..1485
                     /locus_tag="PWY89_002913"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067884.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1737..2309)
                     /locus_tag="PWY89_002914"
     CDS             complement(1737..2309)
                     /locus_tag="PWY89_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2067885.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2325..2930)
                     /locus_tag="PWY89_002915"
     CDS             complement(2325..2930)
                     /locus_tag="PWY89_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL2067886.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3331..>3894)
                     /locus_tag="PWY89_002916"
     CDS             complement(3331..>3894)
                     /locus_tag="PWY89_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL2067887.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT         1292 a          715 c          582 g         1305 t
ORIGIN      
        1 aaataagaaa ttcaaaaaag aaaaaacttt ccttaaaaac aaaaaatagg agtattcagt
       61 ttcttaatag ttttgtaata gttgtatgta gaagttttct taatagtttt tgtaataatt
      121 tattaatcgt agtttatata acttatttgt aagtgactag agaattaaat aaaaaataat
      181 tactatctag tacgaaatta attacatagg ataatatatt aaggtgctgg actacaagta
      241 aaaaatactg tatgatcatg acagattcaa gaaaggagca ataagattgg ttaagatagc
      301 aggactttct aaaggtggaa aaaccgtttc acaactttat aaacaaaact acagagactg
      361 gagacagttc agaaaaaaca ttaaggattc ttactttatt ctaccaacag aacttgaaaa
      421 atatttaccg aataacaaag ctattaattt atatctgtat tactgtttta aggcaaagaa
      481 tgaaggcggg gattcctggc attcggtcaa taccatagca gagtctctag gagtgacaac
      541 taaaactata aacacctgga ataaaacctt agttgattta ggtatgattg cacgtataga
      601 cgataaccat ctcagtaaat cgacaatttt acttcctctc tctagttact ataaattagc
      661 ccttgatagt tctttaaagg atgttaccga gagcactgtc catgaaattg atggcaactt
      721 ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag gctgatgaaa attataacgt
      781 tccttataac acactctgtt gtgtttttaa aagaaaaaca caaaaagata ctatctataa
      841 atttatcttg gtccaagatg agaaaataag taatttcgaa ttaagcactc cttctagtaa
      901 gatttatgac gatgcatata gatttgaatg tcaagatcta gaagccctcc tttccgcaaa
      961 taatctaaac aatatagaac atgaaaactt tgttgtaaac actagattta atttagtttc
     1021 tgaaaaggac tcagatttgt tagatttgct gatagagctt actaaaaatc tagataagaa
     1081 agaaaacatt actgtactat aactagcatc ggtccatcat taagtttgga ctattagaac
     1141 acttgctatt acagaaaaag gtatgctagc cacgcttact atttattggt atcaaacaag
     1201 tatcttaatt tgaaaggaag atgatttatg gctaatttat atatagagaa caaaggagac
     1261 ttgaatgcca ttattacttt aatcgtagtt atggtaaaat caataggtaa tatcacaata
     1321 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
     1381 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
     1441 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
     1501 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
     1561 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
     1621 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
     1681 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1741 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1801 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1861 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1921 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1981 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     2041 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     2101 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     2161 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     2221 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     2281 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     2341 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     2401 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     2461 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     2521 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     2581 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     2641 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     2701 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2761 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2821 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2881 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2941 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     3001 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     3061 gcacagtcta gttgaccttt gatacacctc tatatttata gagaatctaa aacatcaaca
     3121 tggcatactc aaaattctaa atattaggga taaagaattc tcctatacaa tcaaaaattt
     3181 cctttatttc gaactccggt cacctaactc tattgatctc ttacaagcca aagaatatct
     3241 ctatactaca aagaaacaat agggtgcctg gttggtctct ttgtgtttta ttttagaatc
     3301 tagaacgtaa atattcttgt cattctgatt ttatctaaga atatcaatta aatctttttt
     3361 tgcagcctct gaagcgggca ttacagaaaa gaccagtcca ataaccgaag ataccccaac
     3421 cgctagtaaa atagtaaata gatcaagtga cacgtttacc tttatcaaag aaccaacact
     3481 ataagcaaga gccattccta ataaatagcc aattaaccct cctgaaagag ttaaaatcaa
     3541 accttctaaa aggaactgca gcataatcga ttttcgagta gctcctaggg ctcttcttat
     3601 accgatctct tttgttcgtt cagatacaga tatatacatc atgttcatta cacctacccc
     3661 tgcgataaat aaagatatac ctgctacagc tgttataaaa taagtgatcg tactgaggat
     3721 ttgaccaatt cctttcgtta acatagccgt atctaacact tcatattccc ccaaatgttt
     3781 caaactacct ttttctttca attggtttat cactttagag gtcaccttgt caggttcaac
     3841 tccttcctct aaagttaacg ttagagacga tgtatccttt tcagacttaa aata
//
LOCUS       DANKBF010000139         3863 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000139 DANKBF010000000
VERSION     DANKBF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(245..466)
                     /locus_tag="PWY89_002917"
     CDS             complement(245..466)
                     /locus_tag="PWY89_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067888.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="PWY89_002918"
     CDS             complement(564..809)
                     /locus_tag="PWY89_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067889.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="PWY89_002919"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="PWY89_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="PWY89_002920"
     CDS             complement(2033..3034)
                     /locus_tag="PWY89_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL2067890.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="PWY89_002921"
     CDS             complement(3193..3459)
                     /locus_tag="PWY89_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067891.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="PWY89_002922"
     CDS             complement(3449..3805)
                     /locus_tag="PWY89_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2067892.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DANKBF010000140         3781 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000140 DANKBF010000000
VERSION     DANKBF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..2232)
                     /locus_tag="PWY89_002923"
     CDS             complement(<1..2232)
                     /locus_tag="PWY89_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067893.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="PWY89_002924"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="PWY89_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1101 a          806 c          581 g         1293 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatatataa aaataacaat aacctttgtg atttttcttg
     3601 tttgaagaat gttagaagaa aaatctctaa atacttataa ttattttata agcaaaaaaa
     3661 tgctatcggt agtatcaccg atagctgagg aaagtcacag tagggactat ttgattaaaa
     3721 aatatgttta ttcaatccat ctatttattt ttgccttatt aacatattgt aataatgtaa
     3781 g
//
LOCUS       DANKBF010000141         3664 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000141 DANKBF010000000
VERSION     DANKBF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..277
                     /locus_tag="PWY89_002925"
     CDS             <1..277
                     /locus_tag="PWY89_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HDL2067894.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="PWY89_002926"
     CDS             420..641
                     /locus_tag="PWY89_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067895.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="PWY89_002927"
     CDS             657..1499
                     /locus_tag="PWY89_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067896.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="PWY89_002928"
     CDS             1562..3364
                     /locus_tag="PWY89_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDL2067897.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="PWY89_002929"
     CDS             complement(3423..>3664)
                     /locus_tag="PWY89_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2067898.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DANKBF010000142         3449 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000142 DANKBF010000000
VERSION     DANKBF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(150..1337)
                     /locus_tag="PWY89_002930"
     CDS             complement(150..1337)
                     /locus_tag="PWY89_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL2067899.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1447..1824
                     /locus_tag="PWY89_002931"
                     /pseudo
     CDS             1447..1824
                     /locus_tag="PWY89_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /gene="yfcC"
                     /locus_tag="PWY89_002932"
     CDS             1872..3272
                     /gene="yfcC"
                     /locus_tag="PWY89_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2067900.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            3305..>3449
                     /locus_tag="PWY89_002933"
     CDS             3305..>3449
                     /locus_tag="PWY89_002933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067901.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          978 a          652 c          684 g         1135 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgctttta
//
LOCUS       DANKBF010000143         3200 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000143 DANKBF010000000
VERSION     DANKBF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            301..585
                     /locus_tag="PWY89_002934"
     CDS             301..585
                     /locus_tag="PWY89_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067902.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="PWY89_002935"
     CDS             588..1004
                     /locus_tag="PWY89_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067903.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="PWY89_002936"
     CDS             complement(1141..1860)
                     /locus_tag="PWY89_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2067904.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="PWY89_002937"
     CDS             2513..2608
                     /locus_tag="PWY89_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2067905.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..3156)
                     /locus_tag="PWY89_002938"
     CDS             complement(2911..3156)
                     /locus_tag="PWY89_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067906.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DANKBF010000144         2634 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000144 DANKBF010000000
VERSION     DANKBF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            6..1150
                     /locus_tag="PWY89_002939"
                     /pseudo
     CDS             6..1150
                     /locus_tag="PWY89_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="PWY89_002940"
     CDS             1321..1779
                     /locus_tag="PWY89_002940"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2067907.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="PWY89_002941"
     CDS             1938..2408
                     /locus_tag="PWY89_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2067908.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="PWY89_002942"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="PWY89_002942"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          863 a          504 c          497 g          770 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc aacccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatttgaa gtca
//
LOCUS       DANKBF010000145         2461 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000145 DANKBF010000000
VERSION     DANKBF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..189
                     /locus_tag="PWY89_002943"
     CDS             <1..189
                     /locus_tag="PWY89_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2067909.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="PWY89_002944"
     CDS             complement(1055..1585)
                     /locus_tag="PWY89_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HDL2067910.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="PWY89_002945"
     CDS             complement(1582..2199)
                     /locus_tag="PWY89_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2067911.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          810 a          469 c          274 g          908 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 t
//
LOCUS       DANKBF010000146         2400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000146 DANKBF010000000
VERSION     DANKBF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            207..2069
                     /locus_tag="PWY89_002946"
     CDS             207..2069
                     /locus_tag="PWY89_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2067912.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="PWY89_002947"
     CDS             2069..>2400
                     /locus_tag="PWY89_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2067913.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DANKBF010000147         2395 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000147 DANKBF010000000
VERSION     DANKBF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(263..562)
                     /locus_tag="PWY89_002948"
     CDS             complement(263..562)
                     /locus_tag="PWY89_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2067914.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            913..1545
                     /locus_tag="PWY89_002949"
     CDS             913..1545
                     /locus_tag="PWY89_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2067915.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1668..2306
                     /locus_tag="PWY89_002950"
     CDS             1668..2306
                     /locus_tag="PWY89_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2067916.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          808 a          377 c          339 g          871 t
ORIGIN      
        1 ttttcaatct atatttccaa tttttaactt acaaaaatgc aacattaata aataacgata
       61 gtttcataaa atacactttt aattaacatt attagtttat actagctgtt tttgtgttaa
      121 ttaaaggata aaatacaata taaagaaaca tctgtttctt tatttagaat aaaaaaatac
      181 agactctgtt caaataaatg aacctagcct gcattatgag attgactata ctttttaatt
      241 cattgagatg aataactcgt ttttataaat gcccaaaagt aatcggtgcc gaagccattc
      301 ctcgacctaa ttctttttca aattttccta ttgttgatat ctccatataa aacggtgata
      361 ctggtttaga caattctccc ctgattgcta acggacgtaa actacgttcc aaattattta
      421 tgactttagg ataatactga cctttttcta agcctgtctt tgcgtctatc aaaagtatac
      481 gttccttttc tttgatctct ggatttaaaa cgagatcata taattttagt accatgtttt
      541 ctatttcttc acttttagac atcacgattt cctcccaaaa atatatttct tcccactaca
      601 ttctataaac aaagtatacg ctacatctag atttttttaa atcattactg gattgttaaa
      661 acctttcaat acactgttat ggcaacgatt tttattttaa aaacattcta aattaaatca
      721 gtaaaaattt ttattctaag atttttatta gaattacgcc taatttgtgt ctctcagggc
      781 aattctaaga acgtttcaag agatagcgac actatcgtta tcgaaaaagg cgaaaaccta
      841 tctcattttc tgctaatgta agttggttta gcttttgtta atatgacttt atgaaaatgg
      901 aggtaattaa atatgatttt tcctaaagtt aatgaagcaa gcttgaataa aggatccttt
      961 tttcatatct atgaaaacga agattttgaa ctgtataata tcctttcaca aaaagaggtc
     1021 atcgaaaacc aaataatctg ccatccaatt atttacaaat ctattttcaa gtatgcattt
     1081 tttgcttcca atgattttca aagaatggag attaccgact gtatattcga aaactgtgat
     1141 ttttcaggct gtatattgac aaaaagcatg ttgcatcgcg tgaaatttgt caattgtaaa
     1201 ttaacaggta ctaaattgat ggaaagttat attgggaaca cgttattcga gaattgtaaa
     1261 atggactatg taaatttatc tggttcaaac ataaaagaaa gtacctttga gcactctgtc
     1321 ttatcaagcg cagattttgt agactgttcc ctcactaaaa taatgttttc tactaatgat
     1381 ttagaaaatg ctagcttttt tgatactgat ttaaaaaata tcgatttaag tagcaactcc
     1441 tttgaaatga ttgaagtacg ctcggaacat ctaaaaaatt gtacgcttaa tcagttgcaa
     1501 gcacttggtt ttgctagaaa atttttacaa ataaaagtag tataatcaaa atgaatatat
     1561 aagataaatt tttagcaata caaatatcca taaaaaaaca ttgcctattt taactcttca
     1621 tgtatactga ctctatcttc aaaaactgaa tagtaggagt tattatcatg aataacattt
     1681 taggaaaaaa aatagaactt aaaaaaggga tattgggcac tattgtcgtt gtactcggtt
     1741 tgttagggtt gctatttgcg ccatccatta ttagcatttt ttctttaact atcactggat
     1801 ttatagcatt tacacttgtt tttggtaaaa aagatacgaa aaaaatgttc tcgaaacctg
     1861 tcgctcctgt caaaaatata gccaaatatt tttttatcaa tgtagtaatc agtgccgcag
     1921 tctcggtatt tttgcaacaa attttgaaat ggggtttaac aggcaatcca ataaatgagg
     1981 ctttcagccc gttattattt attattttgc ctatcatgat acttggagaa gaattgttct
     2041 cagtgtattt tctcgctatt ttttctagta agttcagcat tcctgtcgct agtttcttgt
     2101 cagcaatcat ctttggattt atccattaca gtacttatga taatggaaat attttacaca
     2161 ctgtagcaca catccttttg atacaagggg tggcaagatt gttattcaac caagctgcca
     2221 ttaaaagcaa ctcgatcatt acaagttggg cagttcatgt gatatttgat ttcacagcta
     2281 tattgctagt cgttttattc tcctaaattt ttatagaaat aatacatgct ctattgctaa
     2341 gaaagcatac tttatttcct ttaaacaaaa agacagatgt atcaatggat taaaa
//
LOCUS       DANKBF010000148         2271 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000148 DANKBF010000000
VERSION     DANKBF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            192..446
                     /locus_tag="PWY89_002951"
     CDS             192..446
                     /locus_tag="PWY89_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2067917.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="PWY89_002952"
     CDS             447..764
                     /locus_tag="PWY89_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2067918.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..2258
                     /locus_tag="PWY89_002953"
     CDS             1323..2258
                     /locus_tag="PWY89_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067919.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          430 g          711 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatgaatatt acaagtaaca aaacgtcacg tgtgctattg gcgttgatgg
     1801 gttacggaat tctttataga gatgaccaaa atggccagat tctaataaat cgggctgggc
     1861 gttcattatt aatgaaatat agaaaattta taccggaaga ttttcaaaac ttacagttag
     1921 aaaacatgcg tttagaagat ttatttagat tagcagaaga tgataagtat agatttgaag
     1981 ttttgaaaat tcaaaagaat gaactattga ataagttata tcctgagaat gcgataaaag
     2041 cacattttgg tgatgctaat catatgtata gttggttcat tcctaaaaga tttagaggga
     2101 aaatcaatgc tggagatcaa gtgaaagtac tcaatcgaaa tagtacagat gtcgtaatga
     2161 taacagatat ctatgtagaa gatgaagata taatcaaata tatgaagcct gtgataaaac
     2221 ttgttaataa aaataaagga acccgtggtg ctagttaatt ttttcctaga g
//
LOCUS       DANKBF010000149         2259 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000149 DANKBF010000000
VERSION     DANKBF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(148..2145)
                     /locus_tag="PWY89_002954"
     CDS             complement(148..2145)
                     /locus_tag="PWY89_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2067920.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          505 c          411 g          769 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaa
//
LOCUS       DANKBF010000150         2227 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000150 DANKBF010000000
VERSION     DANKBF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..280)
                     /locus_tag="PWY89_002955"
     CDS             complement(<1..280)
                     /locus_tag="PWY89_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDL2067921.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="PWY89_002956"
                     /pseudo
     CDS             742..2124
                     /locus_tag="PWY89_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          654 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaa
//
LOCUS       DANKBF010000151         2039 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000151 DANKBF010000000
VERSION     DANKBF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(581..946)
                     /locus_tag="PWY89_002957"
     CDS             complement(581..946)
                     /locus_tag="PWY89_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067922.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1022..1267)
                     /locus_tag="PWY89_002958"
     CDS             complement(1022..1267)
                     /locus_tag="PWY89_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067923.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1337..1591)
                     /locus_tag="PWY89_002959"
     CDS             complement(1337..1591)
                     /locus_tag="PWY89_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067924.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1755..1967)
                     /locus_tag="PWY89_002960"
     CDS             complement(1755..1967)
                     /locus_tag="PWY89_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067925.1"
                     /translation="MIFQGVITLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIIL
                     SFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          750 a          350 c          365 g          574 t
ORIGIN      
        1 agaatattta gaaattatta attttcgtct gaaaacaaaa aaagaaagcg ttattatttt
       61 agtctatttt caggggggga accttatgat aaaaaaacta tttttactat caatattaac
      121 gctagcaatg gcaggttgtt ctgactctgg cttagcaact gaactgatca caaaatcaac
      181 tgaagccacg acagaggagt taaaatgtgg aaacgatcac agtgactaca gaggcttaaa
      241 tcgacgatgc atgggaatat taggcagaag caattgtaag catcgatgta gtaatcgaag
      301 aaggtgtaga agaagcaaca gaaaaatcaa aagaaagatc aaaagaagca atctgtgctg
      361 tgcatcgtat acagacgtcg aagatgcggt acttttaaac cacgtcagga gatgttcatt
      421 tatgtctagg gaagttggcg atgaaccaaa actagagtac gcttttagtt ttgcacgtga
      481 ggatgaagat aaccctaaaa cttacgacat tttagcttga tctaagatgg ttaaagcttt
      541 ttaatgaagt aagaggagat agaaggcttt tctagttata ttaggtaaga taaattcctt
      601 tttgctccaa atttgctaaa cattctgcta gataaacttc atcgtgttcc ggataaatcg
      661 gcacgttgcg ctctacagta ggtaaatcaa aaaatgcttc gcatgatttg ccacgataca
      721 taggattttt ccactctggt gctgggctgt aaccacgttt ttccatttca tccatgacaa
      781 gctgatgata ttggaataat ctataaggag aatagtgaaa aacataatcc acagtagcat
      841 gtttctttcc ccagccattt cctcttaatg cacagcattc ccgatgttgt cctaaaagct
      901 gttgacgagg aagctttgaa atcaattttt catgccataa tcgcatagaa atgtctcctt
      961 tatatataga ttgttaacct tagacgttaa atgaaaaaac cagcaccaag gctggaaaaa
     1021 atcacttcag tttgcttgct gtttctgagc gaccaattag gtaatcaacg gaaatatcgt
     1081 aaaagtcagc gatatagatt aattcaatga tgcttggaat ttcttctcct tcttcgtact
     1141 tacgatattt gaataaagga atacttaacg cgctagaaac agctacttga gataaagatc
     1201 ggccagttct taagaaattc agacgacaac gtaaagtatc cagtaaaata gcttcttcga
     1261 tactcattta atcacctttc attatagtag aatagttata atgactatag aaagctacac
     1321 atgcaatcaa tagtactcaa tgttttttat ttaaacgtat caaataaaga atgacgatca
     1381 ataaagacaa aaccagtcct gataattctt ctataaaaac actatcgggt tcaaagtagt
     1441 tgtaattaaa aactttccac ataaagtaga tagccggaag agtcaaaata gctaaataaa
     1501 accgttcttt gttccaattc ataagataaa caatacttgc gaccagtgcg accgcaggca
     1561 cccaaaacaa aactctcacc aacgtaatca tgtaacggac tcctcaacga ttcatataat
     1621 aaaagataac atgttattgc atacatcaca aatgaaatgc taggaaaata aaagaagaat
     1681 agatcaatga aaaatgaaaa agcagaacag ttttctgctg ataagcagga actgttccgc
     1741 tgaatgtgtc ttatctatcg agaaaaaatc aatggattga tatctaagta attcaataca
     1801 tataaaaaga ggacgatcgc attcactaaa aaggaaagaa tgatgatccc atagcgaatg
     1861 gaaacatatt taacaaatac ttttgtttga atacgggcat atctttttcc tttatagctg
     1921 agcatcatga caagaagcaa aagggtaatc acaccttgaa aaatcattcc aataaacaga
     1981 attcctgagg tttgataatt taattggaga aaataagctg ctgccgcaac taaatcgaa
//
LOCUS       DANKBF010000152         2019 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000152 DANKBF010000000
VERSION     DANKBF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            100..570
                     /locus_tag="PWY89_002961"
     CDS             100..570
                     /locus_tag="PWY89_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL2067926.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="PWY89_002962"
     CDS             792..1895
                     /locus_tag="PWY89_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2067927.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DANKBF010000153         1993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000153 DANKBF010000000
VERSION     DANKBF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="PWY89_002963"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="PWY89_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2067928.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="PWY89_002964"
     CDS             852..1040
                     /locus_tag="PWY89_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2067929.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="PWY89_002965"
     CDS             1053..1610
                     /locus_tag="PWY89_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2067930.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="PWY89_002966"
     CDS             1642..1884
                     /locus_tag="PWY89_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2067931.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DANKBF010000154         1979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000154 DANKBF010000000
VERSION     DANKBF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(325..528)
                     /locus_tag="PWY89_002967"
     CDS             complement(325..528)
                     /locus_tag="PWY89_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067932.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="PWY89_002968"
     CDS             complement(728..1744)
                     /locus_tag="PWY89_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2067933.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          555 a          461 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc cgattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DANKBF010000155         1932 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000155 DANKBF010000000
VERSION     DANKBF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            202..1167
                     /locus_tag="PWY89_002969"
     CDS             202..1167
                     /locus_tag="PWY89_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2067934.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQ
                     LRRGKATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYK
                     YRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
     gene            1367..1570
                     /locus_tag="PWY89_002970"
     CDS             1367..1570
                     /locus_tag="PWY89_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067935.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINSLSGRK"
BASE COUNT          640 a          288 c          436 g          568 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta atcacactag
       61 ctccgtaact tcttaatcat tatacgaata tttttagatt tcttcaagac tagagatttt
      121 ttggtaatga atttttaaga gttacgtgaa cttttgaaaa ttctttccag aagtctcttt
      181 ttctttgagg aggaacaaaa tttgagtaaa aatggcataa tcgtaaatga gtgggaaaat
      241 ttaacagccc tatctaacag gcggttaaat gttcaaaatt tgaacacccc cctaaaatta
      301 cagtggtcaa ttgaccgaat tactatcgtt ggaaaactga aggaaaatat ttattatcac
      361 actcaaaatg atgtgttgat tttagatttt gagcaattaa tgcgggtgaa tgaaggcaat
      421 ggctatttaa aagcggttgg gaataacggc tggcaattat tagaccaata cgaagaaaat
      481 attgcttata ttgagatttt gaaatggcaa gatggcaaag ggcggattga ttttaacccg
      541 aataagattg gtcatttttt agctagttca atgaaaaatt tcattcatga tttatttcta
      601 gaaccacatt tctcacgtgc ggatattgct tgcgatatta tcgatgtgcc tgatgagttt
      661 attacacaat atcgagtggt tgatcctgtt tctttcaagc caatttatgg gcggtctggg
      721 aagctggaaa cagcttattg gggcagtcgt gccagcgaac gacaaattcg gctatacaat
      781 aaaaaattgg aacaagaaac caaacgaaaa gttgtaccaa aggaagttga gacttggtgg
      841 cggttagaaa tgcaactacg gcgtggcaag gcaaccgatt ggcataatat ggttcaagaa
      901 agtttgaata gttttgcgag cctgcatttt ttgccgattg atacaacacc tacagataga
      961 ataatgctcg ctggtttaat gtctgatcaa aatttctggt ctcttttgtc gaggaattca
     1021 aaatataaat atcgggaatt gctgaaagaa gaaagtcaaa atgatgaatt gacgaatcat
     1081 ttgcgagaaa cgtttgcaga atcggcagac aatttgaaac aagaattaga cacgtggctt
     1141 ttgggtttgg acgtgacaga gaagtgatac cgaataagaa aagtcagatg agtgagagta
     1201 aatttacgtc aatgtttaat gacgtgagtt tacgataagc gattattttg cgtgaagcaa
     1261 aataaagcgt aaaaggagtt gagagcgtga cacgctttcg gctccttttt tcgttgtcac
     1321 ttgtgacagc gaataccctc ggagagcatt tggaattgtg acagccatgt cacaattagg
     1381 aaaatgttac tctttgtcaa agagtaacag aaaggtttac actcttttta ttagcgacag
     1441 cgcgaaggag gggtttaact tggggtttag tatttcgttc aaaaagggaa caaaaaatac
     1501 aagcatttct cataataatc gtgagttaac tgatagccaa aaaattaata gtttgtctgg
     1561 tagaaaataa ttagcagatt tacaagtggg aaataaaaag ctgaaagaag aaaattgcat
     1621 ttcaaactac attttctaca ataactctaa aatggcatat tttgcgttct gagcggtttt
     1681 agcgtttatt tcgtttagtt atcggcacaa tcggtgaaac acgttagagg agcgaaaatg
     1741 gagcctgtaa ggcgactggt agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa
     1801 cgaaataaat gctggcagcg ccattctatt tcggcaggag gaggctcaag gggttcgggg
     1861 aatgaaattc cctgaggtct ttttattttt tctagaggag tgctataatc aaagaaaatg
     1921 agaaaaaaga gt
//
LOCUS       DANKBF010000156         1915 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000156 DANKBF010000000
VERSION     DANKBF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..435
                     /locus_tag="PWY89_002971"
     CDS             <1..435
                     /locus_tag="PWY89_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2067936.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="PWY89_002972"
     tRNA            complement(759..842)
                     /locus_tag="PWY89_002972"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="PWY89_002973"
     CDS             1370..1870
                     /locus_tag="PWY89_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2067937.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          618 a          362 c          328 g          607 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatca
//
LOCUS       DANKBF010000157         1893 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000157 DANKBF010000000
VERSION     DANKBF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..355
                     /locus_tag="PWY89_002974"
     CDS             <1..355
                     /locus_tag="PWY89_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067938.1"
                     /translation="DNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCRTCIQ
                     KAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQSIIGV
                     KTLAYLSGMGRNRRF"
     gene            673..1263
                     /locus_tag="PWY89_002975"
     CDS             673..1263
                     /locus_tag="PWY89_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HDL2067939.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1290..>1893
                     /locus_tag="PWY89_002976"
     CDS             1290..>1893
                     /locus_tag="PWY89_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2067940.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAAL"
BASE COUNT          675 a          212 c          360 g          646 t
ORIGIN      
        1 agataatgag ttcgattgta atattgaatt agttaaaaaa attgaaggcg atttaagcta
       61 tatattagaa aatgagatat ttgtaaacaa aacaaggaga cttttgttgg gacgctgtcg
      121 tacttgtatt caaaaggcag ttagatatgc tagttttaat aaagaatttg atttaaactc
      181 ctatttgtct aagatactta tgaacgatga gttgattgcc tgtattcact catacccata
      241 taaacaaaat ccaattaaac aacgaatatt taactatttt ttactaaacc aatccattat
      301 tggggttaaa acattagcat atttaagcgg aatgggtaga aataggagat tttaatcata
      361 aaaaactgta ctatagtgaa tttttggaga tttatattcc agttatatat taatgattgt
      421 gatacttgta ctattgtaaa aattcgttag gtttaaataa ctggaaatat cggaaaatgg
      481 gcattataca tcacggacat cccttctttt ggaaaaaatg ttacgatttt tataagtaat
      541 attgtatccg gtggaatcaa cgatgaagat aacatatcca tcgttgtaga ttcagtagca
      601 ttgaatgatg ctcttgttgc aagaaatccg aataaaattg ttaagtataa attgaattaa
      661 taggagatat gaatggaaat tagcagtaaa gaatatttaa aagcggttct tcgtatggaa
      721 aagaatctat atatagaatc taaaggacaa cacacaaaat tactaaaaat aatgaaatca
      781 catccggact acaacgcttg gcggtatact cgtcgaatga gaattacatc ttattattat
      841 tcaaagagaa agaaaaatat catttatgcg attatgtata ttgtgtcttg cagaagaatg
      901 aatagtttgg gaagaaaatg tggtatagag tgtggtgaaa atgtatttga tgaaggtgta
      961 agattttacc atacacaagg aattgttatt aatggtaacg ccataatagg aaaaaattgt
     1021 tacttatatg ggaataactg tattggaaat gatggaatcg atcctaagtg tcctgtaatt
     1081 ggaaataatg tacgtgtatg cgtgggtgca aagattattg gtaatgtaaa aatagctaac
     1141 aatgtagttg ttgcggcagg agctattgtt atcaaagatt gtctagagaa tggtgcaatt
     1201 cttgcaggtg taccggcaaa aataataggg tatgttaatt taacaaaagt atttttagaa
     1261 taatttttag cgaaagaagg tgaataacga tgcgtatttc aataaaaaga tggtcaggag
     1321 cagattggac attgttcacg ttggcatttt ttgcaatctc catagttctt gtatatactt
     1381 ttaacgcccc aagagcaatt ttatatttgg gagatataat taatatcttt gtgttattaa
     1441 atgcacttag ctttagacgc agaaaaggaa tatatgctag caaaaatgag atgctgatag
     1501 ttttgttttt actagtttct atttgtagct cagttattaa tttagaaaat ataggattga
     1561 ttatctgggg gatacgaaat aacactcgta tgttcatagt atatataaat tgtgcaacat
     1621 atttaacaat taaagattac tataaagtat tcgctataat tgaaaaatta ttctggattt
     1681 cactgccact ttgtttaatt gaaagattca tggtcaccta tccaactgga acaattgtag
     1741 gagatatgat aggtggagtt ttttggaatt attcaggttg taatatgcca ttgaatttaa
     1801 ttttgtgtgt gacagttact gttgttacaa tcggatattt caagggagaa aaaaatttca
     1861 tccattttat tttatgtgtt tgtgcagcac ttt
//
LOCUS       DANKBF010000158         1858 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000158 DANKBF010000000
VERSION     DANKBF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            206..403
                     /locus_tag="PWY89_002977"
     CDS             206..403
                     /locus_tag="PWY89_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067941.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            509..991
                     /locus_tag="PWY89_002978"
     CDS             509..991
                     /locus_tag="PWY89_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067942.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1049..1645)
                     /locus_tag="PWY89_002979"
     CDS             complement(1049..1645)
                     /locus_tag="PWY89_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2067943.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          580 a          275 c          369 g          634 t
ORIGIN      
        1 taattagttt ttaaggaata tgttttaatt ttttgaaaaa aattcttcta aatacaacag
       61 ctgttttaat tgtgaaaacg aaaacagtac gctataatgc ctgtaaggtt gatttttaac
      121 tgtaaataaa aatcaatgag ttaaatctcc aaagaaatag cagtggtaat gtggagaaaa
      181 gttccagcag tttaggagga ttttgatgac ggaagaaatg attaatttag gagaacaata
      241 tgcatgcaaa ccaattggtt ttacaaaaac ggtcattggt gaagttgtat caaaaatgac
      301 aaactgcgcc gtggtcaaag ttgcccaatg tgctgcagag gaccaagaat tattagatga
      361 aaaagcaagc atggttgttg caaaatatga tacatttgaa taaagaaaac aattggacgt
      421 gcggcgtgct gcaagtccaa tttctttgag aaggtaaatc agtattcttt tcctattttc
      481 tggagaagaa aaagcgaggc gagttgtcat ggtaacattc tttacggttc tattgttttt
      541 attcgttgtt gtgatgtttt tatttctttg gctggttcga aaagaaatta tttatcggac
      601 tgtgcgaaat cgatgggtct acttggtgat acccttctta gtgtcactag tcatttggta
      661 tacactcatc agtcatccta cagcagatga attggcaaaa gggatcttgt cagcaatgat
      721 ttttattagt tttttgttgg acagtagagg aatcacagaa gaaggacttg ttttgaatag
      781 ctttgacaaa aaaggtgttc ctttttctga gatcaataaa attgtattat atcagccaaa
      841 aggttctaaa atcgtaaaaa tgaatttttt ccgaaacgga tggcgaggac cgatgctgaa
      901 attttctgca tcattagaag aattagttcc atttttgtcc caacgcttaa acgatgaggc
      961 agagattgat attatgattg atccagaata aaaacagagg ttgcgacaag atgtttgtca
     1021 caacctctgt tttttatttc aaataaaatt atccaaatac gcggaagcga tcggcatcgt
     1081 tcatgtattc tttttcctca gccggtgttt ctggtgaacc gaatactaat tgcgaacgta
     1141 gtttccagtt tgaagggata ttccattctt ttgcaactgc ttcgtcaata actgggttgt
     1201 aatgttgcaa gtttgcgccc aatcctttat caactaatgc tacccaagta ttagctgttg
     1261 cgattccgtt tgattgttct gaccaatctg ggaagttgtc tgcatataat tcaaattgtt
     1321 cttgtagacc ttttaccacg tcagtatctt tgaagaataa tactgtacca taaccatttt
     1381 tgaaaccagc taatttgttt tgtgtatttg ggaaagcttc tgctggagtc aatgggcgaa
     1441 gtgcttcttc agtgatttcc caaagttttt catgtgcatc tccgaaaagg ataactgcgc
     1501 gtgttgattg agcattgaaa gcagttgggc tttctttaat tgcttctttg atcaaagttg
     1561 ttaattcttc attagaaagt gaaacattac gtcctagatg atagattgaa cggcggtttt
     1621 tcaaagtgtc agtaaaagtt gtcataataa aatttctcct ttgatttgcg ttttgcattt
     1681 ttttaattga ttacgaaatg aagtatagca ccttacaaaa agtaagtaaa cagatatgct
     1741 cacaaaaaat aagcgaagtt caaaaaacaa ttttaataag aaaaatcgtt atgtaaggat
     1801 tttcttagtt atcgctttca ttgttgtgtt ttaggaattt ttgcttattt gaaaaaaa
//
LOCUS       DANKBF010000159         1818 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000159 DANKBF010000000
VERSION     DANKBF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            275..1753
                     /locus_tag="PWY89_002980"
     CDS             275..1753
                     /locus_tag="PWY89_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2067944.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          661 a          253 c          324 g          580 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaag
//
LOCUS       DANKBF010000160         1731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000160 DANKBF010000000
VERSION     DANKBF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..156
                     /locus_tag="PWY89_002981"
     CDS             <1..156
                     /locus_tag="PWY89_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2067945.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="PWY89_002982"
     CDS             169..1077
                     /locus_tag="PWY89_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2067946.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1697
                     /locus_tag="PWY89_002983"
     CDS             1077..1697
                     /locus_tag="PWY89_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067947.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          613 a          251 c          395 g          472 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaga atacagaggt tatggaagcg actatagttg aaaatggtct tccctacgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgtaggtg ggaaatatcc agtccaatta
      361 gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaact atatttttga taaatattcc
      421 atgcagtgtt tacatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ttattttgtc attaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aatcgaagat ttaattgcgg atatgacgga ctttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgaacgttgg
      661 agacaagaac aggaacacag tttccaagat tggagagaag gacaagaatc agactatttg
      721 gcatggtttg aatcaatcaa ggatatttta aagacgattg atccaggtgg agtcatgtta
      781 gccgaactaa cggatgcgcg agtagatatt caaggagttc gtcatgcgtc tatttcagag
      841 cgtttgctgg cagatatgga ctatttatat cagaaattgc gagcaacgct tttcacgatt
      901 gaatacgggg agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcat
      961 gaagtcgaaa aagttggaac tgtagcattc ccgattgaag aaggagcctt gatcatcgca
     1021 accgttgatg atccaaagca aaatgtgttc actcttgaga aagttggggt gatctaatgg
     1081 ctaaaactaa acgaatgatg gaaacggatg aaaaaacggg tgtacaacgc caattctttc
     1141 caatcacaca tgcttccgca gttcttgggt tagaagaaat aatagcagga gaagcaacag
     1201 ttttatctgt taatggaaaa attggagccg tcgtcattac taaagaagat ttgggattag
     1261 agaatgttct cacagaatta ccctatgcaa gtgaagaaga tgacggcatt atcacggcag
     1321 aaatgtatca aaaaatttta aacagtggag aaggtgacta cgtgttgcca gttgccacta
     1381 tcgaccgttt gggtggcata aaagttggtg aattattgac gattgatgag acagggaaag
     1441 tctctgcagt cagacaatct gatgtcaatt tttcgctgga gttaaaagaa aaactcgatt
     1501 cactgaaaaa ttatactgct ggagaaaaca tcactattga tgaagatgga acaattagtt
     1561 ctacaggtgg tagtgggact ggtggtgtca atcagtcata tgtagatcaa aaattccaag
     1621 aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa
     1681 aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc a
//
LOCUS       DANKBF010000161         1728 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000161 DANKBF010000000
VERSION     DANKBF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..156
                     /locus_tag="PWY89_002984"
     CDS             <1..156
                     /locus_tag="PWY89_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2067948.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="PWY89_002985"
     CDS             169..1077
                     /locus_tag="PWY89_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2067949.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1694
                     /locus_tag="PWY89_002986"
     CDS             1077..1694
                     /locus_tag="PWY89_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067950.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          629 a          231 c          368 g          500 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaaa acactgaagt tatggaagct acaattgtcg aaaatggtct tccatatgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgttggcg gaaaatatcc agtccaatta
      361 gaaacaagaa ttgtggatgc taaaaaaggg aaaatcaaat atatttttga taaatattcc
      421 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      661 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      721 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      781 gccgaattaa tggatgcacg tgtagatctt caaggtgtac gccatgaatc gatttctgaa
      841 cgtttcctat ccgatctaaa ttatttgtat caaaaaatga aagcagcact tttcacgatt
      901 gaatacggtg aaattgaagt gactgatatt cttcaagacg atctcttttc agataatcac
      961 gaagtcgaaa aaattgaaac agtagaattt ccaattgaag aaggagcttt gattattgca
     1021 actgttgatg atccaaaaca gaatgttttc actcttgaga aagttggggt gatttgatgg
     1081 ttaaagtaaa gagaatgatg gagactgatg aatctggtgt taaacgtcaa tttatgccaa
     1141 ttacacatgt ttctgcagtt ttaggcttag aaaaaatgat atcaggacaa tcaaaagttc
     1201 tttcggttaa tggtaaatat ggtgcagtca ttttaacgaa agctgattta ggattagaga
     1261 atgcaattac tgaactacct tatgcaagcg agacaagcga tggtattctt actgctgaaa
     1321 tgtttcaaaa gatcgtaaat ggagaaggtg gaacatatat tttgccgatt gctactcctg
     1381 aacaattagg aggtataaaa gttggggaac tcttagaagt tactgaagaa ggtgttttat
     1441 ctgcaactaa gcaaacagac ttcaatttct ctgaagaatt aaagggaaaa ttagaatctc
     1501 taaaaatatt aaaagcaggt gcaaatatat caattgcaga agatggaaca attagttcta
     1561 caggtggtag tgggactggt ggtgttaatc agtcatatgt agatcaaaaa ttccaagaag
     1621 ctgtaaatca agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa
     1681 tcggggaggt atagaaaatg acagatatcg taaaaataaa acaaagcg
//
LOCUS       DANKBF010000162         1664 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000162 DANKBF010000000
VERSION     DANKBF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            131..505
                     /locus_tag="PWY89_002987"
     CDS             131..505
                     /locus_tag="PWY89_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2067951.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PWY89_002988"
     CDS             complement(619..1248)
                     /locus_tag="PWY89_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2067952.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PWY89_002989"
     tRNA            complement(1382..1454)
                     /locus_tag="PWY89_002989"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DANKBF010000163         1623 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000163 DANKBF010000000
VERSION     DANKBF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..969
                     /locus_tag="PWY89_002990"
     CDS             <1..969
                     /locus_tag="PWY89_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2067953.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="PWY89_002991"
     tRNA            1159..1232
                     /locus_tag="PWY89_002991"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          538 a          273 c          317 g          495 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agc
//
LOCUS       DANKBF010000164         1601 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000164 DANKBF010000000
VERSION     DANKBF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            91..1557
                     /locus_tag="PWY89_002992"
     CDS             91..1557
                     /locus_tag="PWY89_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL2067954.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          445 a          292 c          333 g          531 t
ORIGIN      
        1 gtttatattt cttaaataag ttttttagtt aaatttttcg gtaaagatgt ataatatttt
       61 ctgaaaattt aaagaattgg ggtatagatt atggaaaaac agcaagacaa gggcttcttc
      121 gggcagccga agggattgtc aactttgttc ttcactgaga tgtgggaacg atttagttat
      181 tacggtatgc gtgcattact tatctattat atgtacgata cagttgccaa cgggggactt
      241 ggattaccac gagcaacagc attagctatc atgtcgatct acggatcttt gatctacatg
      301 tctagtatcg tcggcggatg ggtatctgac cgagtgttag gagcaaaacg cactgtattc
      361 ttcggtggag ttttcattat gcttggccac atcgtcttgg ctttaccatt tggtgtaagt
      421 acattgtttg tttcgatggc attgattatt atcggaaccg cctttttgaa accaaatgtt
      481 tctggcatgg tcggacatct ttattcaaag actgacttgc gaagagatgc aggattttcg
      541 attttttaca tgggtgtcaa cttaggggca ttattagcac cactaatcgt tggtacaatc
      601 ggtcaagaat ataactatca tttagggttc tctttagctg ctattgggat gttctttgga
      661 ttattacaat acatcatcca aggacgcaag acattagacg gagtgggtac aacaccgccg
      721 aatcctttga caactgaaga acgtaaagga ttcatccgta acttgatcat cgcacttgtt
      781 gttatcgctg cagtattcgg cggggcacaa ctaactggtc atctgacaat tgactttttt
      841 gtcaatacaa tcagtgtatt ggggattatc ttaccgttgt attactttat caaaatgctg
      901 acttcaaaag atgtaactgc tgaagaaaaa ccaagagtat gggcttatat cccattattc
      961 cttgcagcaa tcattttctg gtcattacaa gaacaaggtt catctatctt agctttattt
     1021 gctagtgaac gtacacaatc tacgctattt ggtgtaacga tccaagcaag ctggtatcaa
     1081 tcattgaatc caattttcat ctttattctg acaccagtat tcgtaacgat gtggacgaaa
     1141 cttggtgaac gtcaaccatc tacagtagtg aaattctctc ttggactttt atttgcagga
     1201 ttatcgtact tgctattgat gttgccgggt atgctttatg gtacaagtgg aaaagtaagt
     1261 ccattgtggt tgttgggaag tttcttcatc gtggttattg ctgaattgtg tctttcacca
     1321 gttggtttgt ctgtgacaac aaaattagca ccacgagcat ttgaatcaca aacaatcgct
     1381 atttggttct tagctgatgc ttcttcacaa gcaatcaatg cacaaattgc acgcttctac
     1441 acaccgggaa cagaatctgc ttacttcggt attatcggtg cagtagcaat cgtcggtggt
     1501 atcttgctat ttactgtgaa agaaccaatc aaaaaactaa tgggctctat tcattaatca
     1561 atgaacataa aaacaggaat tgcttatttg cagcagttcc t
//
LOCUS       DANKBF010000165         1594 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000165 DANKBF010000000
VERSION     DANKBF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            288..695
                     /locus_tag="PWY89_002993"
     CDS             288..695
                     /locus_tag="PWY89_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067955.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="PWY89_002994"
     CDS             709..1110
                     /locus_tag="PWY89_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067956.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="PWY89_002995"
     CDS             1112..1483
                     /locus_tag="PWY89_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067957.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          596 a          201 c          277 g          520 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtcc
//
LOCUS       DANKBF010000166         1536 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000166 DANKBF010000000
VERSION     DANKBF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..>1536)
                     /locus_tag="PWY89_002996"
     rRNA            complement(<1..>1536)
                     /locus_tag="PWY89_002996"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DANKBF010000167         1494 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000167 DANKBF010000000
VERSION     DANKBF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(302..601)
                     /locus_tag="PWY89_002997"
     CDS             complement(302..601)
                     /locus_tag="PWY89_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067958.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(607..837)
                     /locus_tag="PWY89_002998"
     CDS             complement(607..837)
                     /locus_tag="PWY89_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067959.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1087..1287)
                     /locus_tag="PWY89_002999"
     CDS             complement(1087..1287)
                     /locus_tag="PWY89_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2067960.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          532 a          243 c          216 g          503 t
ORIGIN      
        1 aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag tgtgttacgc
       61 tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga aaaaaagtga
      121 gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga ttttatagca
      181 ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa cttaaaaaat
      241 atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta ggtagcctac
      301 tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca taacgccgat
      361 gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat acgtctttct
      421 tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag tagagattaa
      481 ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc ttataaaaat
      541 ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca ttaaaaagca
      601 taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat atcgagtttt
      661 tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt tattttcgga
      721 aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt cgttgctttg
      781 ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt ccatcataaa
      841 gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga atttattata
      901 ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa atcgaattta
      961 ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata acaaacacaa
     1021 cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc tttgcggtac
     1081 agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt
     1141 cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg tatgtcttag
     1201 tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag aaaaacttac
     1261 taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac gatttcgatt
     1321 ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc ggtataactt
     1381 gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta tttttagtaa
     1441 tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa aaaa
//
LOCUS       DANKBF010000168         1422 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000168 DANKBF010000000
VERSION     DANKBF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            37..447
                     /gene="spx"
                     /locus_tag="PWY89_003000"
     CDS             37..447
                     /gene="spx"
                     /locus_tag="PWY89_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2067961.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            461..817
                     /locus_tag="PWY89_003001"
     CDS             461..817
                     /locus_tag="PWY89_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL2067962.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          523 a          209 c          267 g          423 t
ORIGIN      
        1 tgtagaattg gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct
       61 agctgtactt cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa
      121 gagaagaata tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg
      181 actgaaggag gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc
      241 atagatttta acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca
      301 ttattgagac gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat
      361 gagatccggt catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta
      421 caaaacaata acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg
      481 atcagcgcac tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc
      541 ttatcaattt gttgacttag aagaacgcga tctaagtaca gaagaagcag aagaattatg
      601 cagttttgaa gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa
      661 cattgcgatt gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca
      721 taccaatctc ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa
      781 tgagcagttg atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc
      841 actgagcatt tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc
      901 tttttttgac tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa
      961 atctaggtgg ggatatagag ttaaaaatat attgacatgt gtaaatttac atgatatatt
     1021 catttctgtt gttttaagta gcaaagcaat aaaatagtat tttaaaaaac atataaaata
     1081 gttgttgaca tgttagaaat gacatgataa gataactgag ttgttaaaaa agaaccactt
     1141 gtgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata acaaatgata
     1201 agttatgata aatgagttgc tgtaagattt cgaaaaacga aatcaaaaaa acttttaaaa
     1261 aagagttgac aattacttgt ctctctgata tgatataaaa gttgctacgg cagccaaaaa
     1321 cgaatcgggg aacactcctg atgaagtaga cctttgaaaa ctgaacaaag taagacaaac
     1381 caaatgtgta gggcgtcttg attcaattca agacaacaaa ca
//
LOCUS       DANKBF010000169         1396 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000169 DANKBF010000000
VERSION     DANKBF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            139..1071
                     /locus_tag="PWY89_003002"
     CDS             139..1071
                     /locus_tag="PWY89_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDL2067963.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1143..>1396
                     /locus_tag="PWY89_003003"
     CDS             1143..>1396
                     /locus_tag="PWY89_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067964.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESS"
BASE COUNT          619 a          158 c          222 g          397 t
ORIGIN      
        1 ttattaaaaa aagagaaaga agatttaaat gatgaaataa atcaaaagta tgatgaaaca
       61 ttaaaaaatg tctattcttc acttatcgtt tcaaatatag aagaaacttc atcttctgaa
      121 ataaaaaata agctttcttt gttaaaatta aaagaaaagg aatatattat tgacgatgct
      181 gtttctgtcc ctcttatttt aaataaacga gagaaaaata atttaaagaa aaaaatgctc
      241 attccatttg attcagaagt gaaaggatta ctcaataaac taactatttc taatatagac
      301 tcgactaggg aaaaaatcat aaaaatattt gataaaatta ataatttatt caagaatgac
      361 aatgctgaat taaggaaggg tcttttagag tttaaacttc aagaactaga acttcattac
      421 tcttatttag tgaaaattac cgaagaaaaa gaacaacaaa aagcaataaa agaacagatg
      481 atagaagagg aaaaagttcg tagagaaatt gatcgtgaaa agaaacgtat tgataaagaa
      541 caacgtcaat tcaattctga aatttccaaa ttaatcgcat atatgcaaaa agctaatgca
      601 gatgtggaaa aagaattata tgcaaataaa attcaagaac tggaagaaaa attaaaagaa
      661 ttagaagtta tacgtgaaaa tgtattacaa agagaattaa atactagagc cggttatgtg
      721 tatgtaatta gtaatattgg gtcttttggt gaagacatat ataaaattgg tatgactaga
      781 agattagaac ctatggatag aataaaagaa ttgagtagtg catcggttcc atttgaattt
      841 gacgttcatg cgatgatttt ttcagaggac gctccgtctt tggaaactaa attgcataat
      901 cattttagaa aacaagaagt taataaaatt aatcaaagaa aagaattttt caaagtttca
      961 ctagacgaaa tagagaaagt tgttttagaa aattataatg gaacagtaac atttacaaaa
     1021 ttagctaaag ctgaacaata tagacgatct ttagaattgt caaaagatta aatatataaa
     1081 aagccttcgg gctttttctt ttaacaatta ataggaacgt atattcgaaa ggaatagaaa
     1141 taatgtggtt agaaaaaata aatgacaaaa aatttaagta caccgaacgt tatacggatc
     1201 ccttaactga aaaaaagaga aaagtgtccg ttactttatc tagcaattca cgccaagctt
     1261 ggaatcaagc taacttactt ttgaatgaaa aaatcgcaga aaaaataaaa cgaaatgaag
     1321 aattaccact aacttttgga gaattaaaaa ctaaatggga tgaaaaatac aagccaactg
     1381 taaaagaaag ttccta
//
LOCUS       DANKBF010000170         1390 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000170 DANKBF010000000
VERSION     DANKBF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(166..239)
                     /locus_tag="PWY89_003004"
     tRNA            complement(166..239)
                     /locus_tag="PWY89_003004"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(203..205),aa:Ile,seq:cat)
     gene            complement(428..1387)
                     /locus_tag="PWY89_003005"
     CDS             complement(428..1387)
                     /locus_tag="PWY89_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2067965.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          405 a          278 c          254 g          453 t
ORIGIN      
        1 acttttcaaa tatacagaaa tataactatg tgaaacatcc tttcattaat ccataaaagg
       61 atacataaaa aagccactca tttgagtggc aatgaagaaa agctttagct tgtataatac
      121 tcttcaaaaa attctaacac agaacgattc aaatggctac gttaatgtac cctgtaggac
      181 tcgaacctac gaccggacgg ttatgagccg tctgctctga ccaactgagc taagggtact
      241 ggttgttgcc acataaagcc ataaacaatc aaccagtaga atgtgtggca acaaacctgt
      301 tatcgcatag cttggagtgt gactattatg ggtgatagtg aagatatgcg ataacatcac
      361 tattttatcg aatgattttt atagttgtca atatagttat gtaatgctcc tcaacgagga
      421 gctattttta tcgtttagga atatttaaat accaacgttt gtcatggaaa tcttgcgcac
      481 cgcctttagt gtttccctct ggatcattcg ttgcccgcat catgacatag actttcttat
      541 tagggaagtt acgcatgtta aaagatacat gatagccaac atttccagaa gtattataag
      601 cttgatttac atctgatcta taaattccat cagctcttac tcgagctaat tctttcccag
      661 tattgtaatc cataatgaag atatactcgt atttatagtt agcaatgtgc catccagcta
      721 catgcaaatt tgcattttcg atttctccaa actgatcaat gtgggcgtaa ttcgttccat
      781 ctgtcagtgt aggattagct gcaccagctc gtgttggatc aatgacaggc ttgttttcag
      841 aagttgttgg attttcatcg gtaaatccat gagctaaatc atatgctaat ttttctttac
      901 ttacgcccat ttcagaaaga taaccgtaag gatctgtatg atcgccccag atgttttgtg
      961 ttacccataa atgcgatttg attcccggtt ggttataagg agtgtccaac gttaatggaa
     1021 taccatattt cattgctgaa tctctagcca attcaacgta tgccttgtag tttttctcaa
     1081 acgttgcttt atcatgtgtg tgttgtaact caatctgcac aggactgttg gcattagcat
     1141 acgaaccagc accgtactgt acataaccag gttgaccgac ttgataaaca attccgccgt
     1201 ctcccacaat ataagcagta taagcgctag tccatgaacg ttgcatatac tgcgcttcat
     1261 tgcgtcctgt tgctgtttca ttagccgttt catgcagtaa aatatactga ttatttgcta
     1321 cttgtgagct accttcattt gcgcccaaat taaattcatt gttgatagta taggcaaatc
     1381 cgttaattgg
//
LOCUS       DANKBF010000171         1364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000171 DANKBF010000000
VERSION     DANKBF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            82..>1364
                     /locus_tag="PWY89_003006"
     CDS             82..>1364
                     /locus_tag="PWY89_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL2067966.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DANKBF010000172         1361 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000172 DANKBF010000000
VERSION     DANKBF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..208)
                     /locus_tag="PWY89_003007"
     CDS             complement(<1..208)
                     /locus_tag="PWY89_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067967.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
     gene            complement(305..757)
                     /locus_tag="PWY89_003008"
     CDS             complement(305..757)
                     /locus_tag="PWY89_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067968.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(764..1312)
                     /locus_tag="PWY89_003009"
     CDS             complement(764..1312)
                     /locus_tag="PWY89_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2067969.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          487 a          264 c          133 g          477 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
      361 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
      421 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
      481 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
      541 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
      601 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
      661 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
      721 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
      781 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
      841 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
      901 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
      961 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
     1021 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
     1081 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
     1141 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
     1201 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
     1261 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
     1321 ctccataaaa aataagttac tttttaaata tcttgtttat g
//
LOCUS       DANKBF010000173         1294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000173 DANKBF010000000
VERSION     DANKBF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..1244
                     /locus_tag="PWY89_003010"
     CDS             <1..1244
                     /locus_tag="PWY89_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL2067970.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DANKBF010000174         1280 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000174 DANKBF010000000
VERSION     DANKBF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            919..>1280
                     /locus_tag="PWY89_003011"
     CDS             919..>1280
                     /locus_tag="PWY89_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2067971.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSV"
BASE COUNT          467 a          200 c          177 g          436 t
ORIGIN      
        1 gaaacaattt tttctgcttg ttacctaaat taaatcacaa ctaaaggtaa cagtcaacaa
       61 agatttgtta ctaatctaat tttctatttt ttcgccttcc aaatcaagaa aaccgaaggt
      121 aacgtcttat cctagtgtga tacctcaaat gtccactttt gttacctttg ctgtccaaaa
      181 ctgttaccct tactgtccac ttttgttacc tttactgtcc aaaagtgtta cctttatacc
      241 cctttaatac ttactctccc aagagattaa gcctccccta acagatatat taacagatat
      301 ttaacagata tattaacaga tatttaataa aggaggatta ctcctctctt atttcaaaat
      361 cttaaaaata aaaaatctta aaaaaaacta acactatcta ggagtttatt ttgcttgtgt
      421 aactttgaat aattgaaaaa aaggaaaagg aggaagcttc agcttcccct tttatttata
      481 tttataatat atattttaat ctttaaaact ttagcaacct ccaaaaaggc tttatatcaa
      541 tcttaaatag attttagggt ggacataaag cgatgcgaac atggacaaaa agcgatgtga
      601 atgtggacaa aaagcgatgc aaacgtggac aaaaagcgat gtggtattcc gaatatatcc
      661 aaaaaaagaa aaagtatgtg aatttatttc taaaaactag aaaaatattt gttttttaat
      721 tttggtaatt tttataattt cgtattttat tgaaaaggat ttttattttg aaaatgttct
      781 aaaatagcga tttctgctgt attttcaatg aaatttgatt ggaaaaaata aaatgtgttt
      841 tactgacatt gagttatgtt ataaatttac gatacatttc ttattgtaac gtatcgaaaa
      901 caggtctata cttattcaat ggaatcgtac cagttccaag tttttaagct cgtaccaagc
      961 ttagaaagaa gaggcaatcg tatgaaaata acagaaaaga atgtaaaccc gaaatttgaa
     1021 actaccgaaa gcaatctaaa aaagtttgat ttaactgtta ctttatcgaa tcaacttgta
     1081 gatagcattg ttgaaatgga tgctatgcca caaaaaattt atctttacgc tataggacat
     1141 cttgacccta aaaatccgcc ttttaataat accgtcaaag tatctaaaaa ggctttattt
     1201 gcgttgatgt cttctagttc atcttcttca aaacattcaa gattaaaaca agttattgaa
     1261 aaattgcatc agcaatctgt
//
LOCUS       DANKBF010000175         1275 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000175 DANKBF010000000
VERSION     DANKBF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(31..1203)
                     /locus_tag="PWY89_003012"
     CDS             complement(31..1203)
                     /locus_tag="PWY89_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDL2067972.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DANKBF010000176         1269 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000176 DANKBF010000000
VERSION     DANKBF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..1204
                     /locus_tag="PWY89_003013"
     CDS             <1..1204
                     /locus_tag="PWY89_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2067973.1"
                     /translation="EKEYKGVQAQFIKGRLDDSPSHCEHCGSIRIIRNGSYTTRTQML
                     KVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKL
                     IAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTT
                     NQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDR
                     FHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYH
                     RLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTISSTLPQ
                     SFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYL
                     QQGLIFEN"
BASE COUNT          393 a          273 c          249 g          354 t
ORIGIN      
        1 tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa gggagattgg atgactcacc
       61 ttcccattgt gaacactgtg gatcgattcg tattatccgc aacggttcct atacgacacg
      121 cactcagatg ttgaaagtca aagaaaagtt gactatttta gaattaaaac gtacccgttt
      181 tctctgccat gattgtggac ggaccttttc tgcgaaaacg gacttagttg atgaacatca
      241 tcaactaaca aaggaactga agcaggcgat tctcatggaa ctgtacgaaa atcaatcacg
      301 taaactaatc gctaagaagt acttcgtctc ggatgggacg gtgacacgga tcttgcgtga
      361 agcaacgaag cattaccagc cacgtatgaa cttccttccg acggtgttgt gtatggatga
      421 gttcaaatca atgaagtctg tttctggttc catgagtttt atttgtgttg atgggacaac
      481 caatcagcta tttacgatcc ttgaagatcg cagactttac aaactaactc aatacttcct
      541 gcgctttcct agaaaggccc gtttaaaggt caagtaccta gtgatggaca tgaatgccag
      601 ctattgtcag ttgcttaaaa cagtctttcc aaatgcggag atcgtaactg accgctttca
      661 tattgtccaa cacatcaatc gcagcttcaa tcaattacgt gtccagatca tgaatcgttt
      721 tcgaacctca cactctgaaa atcagaagaa gtaccgtcgg ctcaagcgat attggaaact
      781 cttactcaaa gattccacga cattggagcc actcaaacgg cattatcatc gactgtttaa
      841 acgaccaatt agtcaaacag aaatcgttga tgagctgtta agctacaacg aagaattgcg
      901 aactgcgtat catttttgtc aattattacg ctattatttc gttaaacgag atacggaagg
      961 ctttcaagaa acccttaaaa caatttcatc tacgttacct cagtcgttta aaaagaaatt
     1021 cacgatcttc catcgctacc aatcgggtat ttcaaatgcc tttaaggtgc cccactcaaa
     1081 cggcgtcact gaggggctga ataacaagat caagctgatt aaacgaattg cgtttggtta
     1141 tcgcaacttt tataactttc gggcacggat ctatttacaa caaggtctga tctttgaaaa
     1201 ctaaaaaaat atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt
     1261 gtcttcgcc
//
LOCUS       DANKBF010000177         1246 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000177 DANKBF010000000
VERSION     DANKBF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            200..742
                     /locus_tag="PWY89_003014"
     CDS             200..742
                     /locus_tag="PWY89_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067974.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="PWY89_003015"
     CDS             898..>1246
                     /locus_tag="PWY89_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL2067975.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DANKBF010000178         1228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000178 DANKBF010000000
VERSION     DANKBF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            52..>1228
                     /locus_tag="PWY89_003016"
     CDS             52..>1228
                     /locus_tag="PWY89_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2067976.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     "
BASE COUNT          375 a          244 c          242 g          367 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccat
//
LOCUS       DANKBF010000179         1193 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000179 DANKBF010000000
VERSION     DANKBF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..1076
                     /locus_tag="PWY89_003017"
     CDS             <1..1076
                     /locus_tag="PWY89_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2067977.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DANKBF010000180         1137 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000180 DANKBF010000000
VERSION     DANKBF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..355
                     /locus_tag="PWY89_003018"
     CDS             <1..355
                     /locus_tag="PWY89_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067978.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="PWY89_003019"
                     /pseudo
     CDS             615..910
                     /locus_tag="PWY89_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="PWY89_003020"
     CDS             985..>1137
                     /locus_tag="PWY89_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2067979.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DANKBF010000181         1125 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000181 DANKBF010000000
VERSION     DANKBF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            132..344
                     /locus_tag="PWY89_003021"
     CDS             132..344
                     /locus_tag="PWY89_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067980.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="PWY89_003022"
     CDS             506..1024
                     /locus_tag="PWY89_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDL2067981.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
BASE COUNT          418 a          140 c          178 g          389 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaa
//
LOCUS       DANKBF010000182         1120 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000182 DANKBF010000000
VERSION     DANKBF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            120..>1120
                     /locus_tag="PWY89_003023"
     CDS             120..>1120
                     /locus_tag="PWY89_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067982.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPL"
BASE COUNT          383 a          142 c          146 g          449 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt taaaaaaaat aaatgatact atatgtaata taaaaaaaca
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      721 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      781 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      841 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      901 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      961 ttggtattgg cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat
     1021 taggaatctt atctaaaatc acttatataa tgggtataat atctattcta ctttgtacaa
     1081 aaatatttcc atggtttgcc ttacaaaata ctcctttaaa
//
LOCUS       DANKBF010000183         1073 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000183 DANKBF010000000
VERSION     DANKBF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..>1073
                     /locus_tag="PWY89_003024"
     rRNA            <1..>1073
                     /locus_tag="PWY89_003024"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DANKBF010000184         1002 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000184 DANKBF010000000
VERSION     DANKBF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            370..1002
                     /locus_tag="PWY89_003025"
                     /pseudo
     CDS             370..1002
                     /locus_tag="PWY89_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cc
//
LOCUS       DANKBF010000185          991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000185 DANKBF010000000
VERSION     DANKBF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(55..789)
                     /locus_tag="PWY89_003026"
     CDS             complement(55..789)
                     /locus_tag="PWY89_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2067983.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          344 a          206 c          182 g          259 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa c
//
LOCUS       DANKBF010000186          974 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000186 DANKBF010000000
VERSION     DANKBF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(24..97)
                     /locus_tag="PWY89_003027"
     tRNA            complement(24..97)
                     /locus_tag="PWY89_003027"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61..63),aa:Ile,seq:cat)
     gene            complement(652..966)
                     /locus_tag="PWY89_003028"
     CDS             complement(652..966)
                     /locus_tag="PWY89_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067984.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          359 a          152 c          153 g          310 t
ORIGIN      
        1 aacgattcaa ttggctacat taatgtaccc tgtaggactc gaacctacga ccggacggtt
       61 atgagccgtc tgctctgacc aactgagcta agggtagtgg ttgttgccac ataaagccat
      121 aaacaatcaa ccagtagaat gcgtggcaac aaacctgtta tcgcatatct tggagtgtga
      181 ctatttatga gtgatagtga agatatgcgt ctccatatat gtatggttga gacagataaa
      241 catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga gagaaaagta
      301 gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa acatttagaa
      361 atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac ttttgttcgt
      421 acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt atggtttaag
      481 catgttcctt caaataagtt ttttaattat aaactctaga gaaattaatt catcctaaca
      541 taaaataatt gttcactcaa aagtttttct agaacaatta aaacagcagg cttcacattc
      601 gctatacatt tgtaagaagt aataattcct gtactaaaag gcataattgc tttaattatt
      661 ttttgacttc tcttcaaaca caagaagtag ttgtgtaata attgcagaaa taaggattcc
      721 aataatagat ataaaatttg acatggaaac gtctataaca ttaaaagaat aaagtgttga
      781 agaaagtaaa ctgattgaaa taacccctcc gtaaaaaata gtctgtgaat atacccactt
      841 tttttcagaa gacgtacttc gtttggttct aaaacctatc cctgcatttc tactcttatc
      901 aaaaaaccaa atatttagaa gtaataatat tgctattatt aagataaaat tactgtaaaa
      961 atccaaaagc tatc
//
LOCUS       DANKBF010000187          961 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000187 DANKBF010000000
VERSION     DANKBF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            172..459
                     /locus_tag="PWY89_003029"
                     /pseudo
     CDS             172..459
                     /locus_tag="PWY89_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..904
                     /locus_tag="PWY89_003030"
     CDS             473..904
                     /locus_tag="PWY89_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067985.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DANKBF010000188          892 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000188 DANKBF010000000
VERSION     DANKBF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            250..873
                     /locus_tag="PWY89_003031"
     CDS             250..873
                     /locus_tag="PWY89_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2067986.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          327 a          129 c          167 g          269 t
ORIGIN      
        1 ctaatgtcct tatttttgtg ttcacatttc tgttacctaa gaaatactta aaggaggtga
       61 tgtagccagc aacgactaca acttgaaccc gctatctaca gtttatttgt tgtttctttt
      121 gggtatactc taatggtaca aatccatatt tgcttaaagc gttgattttt cagcggtttt
      181 tctttattgc aaagtgtacc ctaagacctg tatactaata gtaaatagat tggataggaa
      241 tgatggcaaa tgtatttagc ttatttaaga gtttcttccg acgatcaaag tttagctcgg
      301 caacataagc taattgaaaa ttggtcagtc aaaaataata ttgctgaaga cgatttaaaa
      361 attttcgagg aaaaagtctc tggaaaaaat attgaaaaca gaccagagct gcaagagctt
      421 atttctttca tacgtgaacg agatacgatt attattccta gtcttgatcg attagcaaga
      481 aatagtaaag atacaaaaga cttacttcaa ttgtttcgta gtaaaggtgc ttctattgaa
      541 atattagacc taccgtcgtt taatggggta actgaccctg cattaagaga cttgctaaca
      601 aatttagtca tagaagtgtt tagctatgtt gctgaaaacg aacggaaaaa aatcaaagag
      661 cgtcaaaaac aaggcattga aattgcaaag aaaaatggtc gttatactgg taaaaagatt
      721 caataccatg ccaatgcaac aggaaaaaac aaattgattt acgatgaaat tgttgatggt
      781 ctgaagaaaa aagaaactat ttctaaaata gcaaagaacg caggagttac aagaaaaacc
      841 gtttatcgag taaaaagaga tttaggaatg taatatgttc cagttagaaa ga
//
LOCUS       DANKBF010000189          886 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000189 DANKBF010000000
VERSION     DANKBF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            136..309
                     /locus_tag="PWY89_003032"
     CDS             136..309
                     /locus_tag="PWY89_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067987.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          297 a          144 c          107 g          338 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaa
//
LOCUS       DANKBF010000190          813 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000190 DANKBF010000000
VERSION     DANKBF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            8..481
                     /locus_tag="PWY89_003033"
     CDS             8..481
                     /locus_tag="PWY89_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL2067988.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            490..717
                     /locus_tag="PWY89_003034"
     CDS             490..717
                     /locus_tag="PWY89_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2067989.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          271 a          123 c          145 g          274 t
ORIGIN      
        1 gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta taagtgccgt
       61 atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa aaggattcaa
      121 ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc tcctagggat
      181 cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc aagctttttc
      241 cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta ttggtttcgc
      301 cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg ctccaggttt
      361 gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat taacaagcgt
      421 gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt tgacagtgtg
      481 aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa aaaaatgagt
      541 gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat tctaaaaaat
      601 ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt tttggagtgt
      661 caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga agaataaaat
      721 aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga gacttttaga
      781 agttatttac gtctattatt cagatttttt att
//
LOCUS       DANKBF010000191          811 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000191 DANKBF010000000
VERSION     DANKBF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..672
                     /locus_tag="PWY89_003035"
     CDS             <1..672
                     /locus_tag="PWY89_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2067990.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DANKBF010000192          751 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000192 DANKBF010000000
VERSION     DANKBF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            10..>751
                     /locus_tag="PWY89_003036"
     CDS             10..>751
                     /locus_tag="PWY89_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2067991.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPG
                     TPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          254 a          131 c          149 g          217 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta g
//
LOCUS       DANKBF010000193          744 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000193 DANKBF010000000
VERSION     DANKBF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(91..>744)
                     /locus_tag="PWY89_003037"
     CDS             complement(91..>744)
                     /locus_tag="PWY89_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HDL2067992.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          203 a          174 c          141 g          226 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gacc
//
LOCUS       DANKBF010000194          725 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000194 DANKBF010000000
VERSION     DANKBF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            28..>725
                     /locus_tag="PWY89_003038"
     CDS             28..>725
                     /locus_tag="PWY89_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2067993.1"
                     /translation="MLGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWD
                     GKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQA
                     AYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQ
                     CYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYD
                     QLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DANKBF010000195          717 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000195 DANKBF010000000
VERSION     DANKBF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..608
                     /locus_tag="PWY89_003039"
     CDS             <1..608
                     /locus_tag="PWY89_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2067994.1"
                     /translation="IGTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGT
                     GVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRL
                     GKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFA
                     KLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          201 a          142 c          182 g          192 t
ORIGIN      
        1 tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg
       61 cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact
      121 atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt
      181 ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg
      241 gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa
      301 aacgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag
      361 cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca
      421 tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg
      481 caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag
      541 cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac
      601 attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
      661 aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DANKBF010000196          670 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000196 DANKBF010000000
VERSION     DANKBF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            154..417
                     /locus_tag="PWY89_003040"
     CDS             154..417
                     /locus_tag="PWY89_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2067995.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="PWY89_003041"
     CDS             417..>670
                     /locus_tag="PWY89_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2067996.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DANKBF010000197          656 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000197 DANKBF010000000
VERSION     DANKBF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="PWY89_003042"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="PWY89_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL2067997.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>656)
                     /locus_tag="PWY89_003043"
     CDS             complement(481..>656)
                     /locus_tag="PWY89_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2067998.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DANKBF010000198          650 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000198 DANKBF010000000
VERSION     DANKBF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..108
                     /locus_tag="PWY89_003044"
     CDS             <1..108
                     /locus_tag="PWY89_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HDL2067999.1"
                     /translation="TLEYDDKLVRKIIQSVTVYEGHFVIAFKSGIEMEI"
     gene            251..475
                     /locus_tag="PWY89_003045"
     CDS             251..475
                     /locus_tag="PWY89_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2068000.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            490..>650
                     /locus_tag="PWY89_003046"
     CDS             490..>650
                     /locus_tag="PWY89_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2068001.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTA"
BASE COUNT          223 a           99 c          135 g          193 t
ORIGIN      
        1 accttagaat atgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa
       61 ggccacttcg tcatagcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta
      121 aatagcccat gactctacag tagagttgtg ggttttcttt tgcttgctga ttataataat
      181 attttaataa ctattgtgtt tatcaaccaa atggtttata atattcttag cgtaaacttg
      241 gaggtgctgt atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag
      301 tgtttccgaa cttgctagac gtattggcca gactccacag aatttcaata aaaaattaca
      361 acgagaaaca gtaaccttgg atgagttgaa ggccattgct gatgtgctag gtgtcaagtt
      421 tgtgcaggca tttattttac cagatggaga agaaataaaa atatctaacg aataaaggag
      481 gatgtttata tggtaggtaa tgttattcaa atagtaacag agaaattatc atctttgcct
      541 tttatagaag gcatcgtatt agggggctca cgtgcaagag gcacccatac agaaaattct
      601 gatatagata tcggaatcta ttacaattca gactcatttg acctgacagc
//
LOCUS       DANKBF010000199          629 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000199 DANKBF010000000
VERSION     DANKBF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..560)
                     /locus_tag="PWY89_003047"
     CDS             complement(<1..560)
                     /locus_tag="PWY89_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068002.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELL"
BASE COUNT          213 a           98 c           89 g          229 t
ORIGIN      
        1 tgaagcaact ctttgcgttt tgacgttaga tcatttatat actctatcaa atagcaataa
       61 aatgctggat agttttctct aaaaaaagaa acatcaagtg gaaattcatc agctttatta
      121 atagtgtaat gcaaaagttt aaaatgtatt ttggttaact ctcttcggat caattggctc
      181 gtctcttctc gcaacagatt aaactgattt tcaaaacttt caatgaatcg ttcattcaac
      241 cattcgattg tttcatcttc attttttctc aagtattttt gatccaattt atcatgaaga
      301 taaagccaag agagtgtctt attgataaaa ataacggatt tttctcctaa tactcctttt
      361 aagtggtaaa atcctatatc cgaaaaaaat agcgactgat tgctagcaat atctaaagaa
      421 tcagcttgtt cagccaacag gttgattgct aaaaagtgga ataatttctt tttttcataa
      481 gcagacagag gataatactg ttttaaatct tttaacattt tttctgtttt tataattata
      541 ggtagcgtat acaattccac aaaacttatc tctgttgagt taaaaagatt atataaaaaa
      601 agccgaatgt ttttaggatt ccctgtcaa
//
LOCUS       DANKBF010000200          593 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000200 DANKBF010000000
VERSION     DANKBF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..580
                     /locus_tag="PWY89_003048"
     CDS             <1..580
                     /locus_tag="PWY89_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2068003.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DANKBF010000201          537 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000201 DANKBF010000000
VERSION     DANKBF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(55..357)
                     /locus_tag="PWY89_003049"
     CDS             complement(55..357)
                     /locus_tag="PWY89_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068004.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          185 a           92 c           75 g          185 t
ORIGIN      
        1 ttataagtat ggtgataaaa tccgttccgg gctacctttt ttaggtagcc tactttaatc
       61 tttatacctt tctggatcaa cgaaagtata cttcaaatag tcataacgcc gatgatcgct
      121 ccgtgcgtct ggcacatcag tcacgatatc aaacaaaaaa tacacatctt tcttcattct
      181 ggtttttgtt gccggaattt taaagtagtt cttattagag taatagagat tgattaataa
      241 gctatcttcg attgctaaaa agaaaacttc tgaatcccac accttataaa aatctttgac
      301 aaatctattc gaaggatcaa atttaaacca taattgtgtt tttccttcca tcaacataat
      361 gttcacctca aaaagagtat acgaactaat gttctttttg taaagtagga atatcattga
      421 taatatggat ggaacatata tattaaaaag tgttcttttt gaatgctatt ttgaatgcca
      481 ttttcattca tttaacgaaa atctgcgaaa ataaaaaatg ctcaaaccct tataaaa
//
LOCUS       DANKBF010000202          530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000202 DANKBF010000000
VERSION     DANKBF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(7..92)
                     /locus_tag="PWY89_003050"
     tRNA            complement(7..92)
                     /locus_tag="PWY89_003050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Leu,seq:taa)
     gene            complement(109..180)
                     /locus_tag="PWY89_003051"
     tRNA            complement(109..180)
                     /locus_tag="PWY89_003051"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Gly,seq:gcc)
     gene            complement(192..264)
                     /locus_tag="PWY89_003052"
     tRNA            complement(192..264)
                     /locus_tag="PWY89_003052"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Thr,seq:tgt)
     gene            complement(282..363)
                     /locus_tag="PWY89_003053"
     tRNA            complement(282..363)
                     /locus_tag="PWY89_003053"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Leu,seq:tag)
     gene            complement(371..443)
                     /locus_tag="PWY89_003054"
     tRNA            complement(371..443)
                     /locus_tag="PWY89_003054"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(408..410),aa:Lys,seq:ttt)
     gene            complement(447..519)
                     /locus_tag="PWY89_003055"
     tRNA            complement(447..519)
                     /locus_tag="PWY89_003055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(484..486),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          115 t
ORIGIN      
        1 ttgaaatgcc gaggaccgga atcgaaccgg tacggtgatc actcaccgca ggattttaag
       61 tcctgtgcgt ctgccagttc cgccaccccg gcgcgattgc tttggcaaag cggaaaacgg
      121 ggttcgaacc cgcgaccccc accttggcaa ggtggtgctc taccactgag ctatttccgc
      181 gtgtttaaag atgccggcta aaggacttga accctcgacc ctctgattac aaatcagatg
      241 ctctaccaac tgagctaagc cggcatgtaa aacttaaatt atgcgggtga agggacttga
      301 acccccacgc cgtaaggcgc tagatcctaa atctagtgcg tctgccaatt ccgccacacc
      361 cgcaaaaata tgagccgtac agggctcgaa cctgtgaccc tctgattaaa agtcagatgc
      421 tctaccaact gagctaacgg ctcatatgga ggttaacggg atcgaaccgc tgaccctctg
      481 cttgtaaggc agatgctctc ccagctgagc taaacctcca tggataactt
//
LOCUS       DANKBF010000203          529 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000203 DANKBF010000000
VERSION     DANKBF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            32..303
                     /locus_tag="PWY89_003056"
                     /pseudo
     CDS             32..303
                     /locus_tag="PWY89_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          198 a           81 c           93 g          157 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttaaatcta tattttgata tacaaattac
       61 agcttcagat actaagttgc ataaaccttt tccggacccc atacaaaagg caattgatga
      121 attgggcata aaaaataaga aacgatttat attggtgatt cattatatga catgcaaagt
      181 gcgaaagctt gtgggatagc atttggttta gctaaatggg gagccaaaaa tatagaaaat
      241 tttcaaagtg tagatataat tattgattct cctcaagaaa tacttgattt gttagaaaaa
      301 tagacaaaga gcgtgagata aggatagggt ctcacgctct ttgtttttcg ccatttaaag
      361 attgaaattt ttgccatcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg
      421 tgctacactc aaaatagttc cgaccaaaga attataagga gtgagtacaa atgacctata
      481 cccatcttac aacggatgaa cttgtaataa tagagtctta tttcaaaat
//
LOCUS       DANKBF010000204          526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000204 DANKBF010000000
VERSION     DANKBF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..354
                     /locus_tag="PWY89_003057"
     CDS             <1..354
                     /locus_tag="PWY89_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2068005.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..>526
                     /locus_tag="PWY89_003058"
     CDS             355..>526
                     /locus_tag="PWY89_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068006.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DANKBF010000205          522 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000205 DANKBF010000000
VERSION     DANKBF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..508
                     /locus_tag="PWY89_003059"
     CDS             <1..508
                     /locus_tag="PWY89_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HDL2068007.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          158 a           92 c          112 g          160 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact ttaactgtta gc
//
LOCUS       DANKBF010000206          517 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000206 DANKBF010000000
VERSION     DANKBF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            57..>517
                     /locus_tag="PWY89_003060"
     CDS             57..>517
                     /locus_tag="PWY89_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2068008.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DANKBF010000207          515 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000207 DANKBF010000000
VERSION     DANKBF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            19..>515
                     /locus_tag="PWY89_003061"
     CDS             19..>515
                     /locus_tag="PWY89_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDL2068009.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          188 a           93 c           86 g          148 t
ORIGIN      
        1 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
       61 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      121 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      181 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      241 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      301 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      361 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      421 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
      481 gataagtatg tcgctatttt tatggacgct actta
//
LOCUS       DANKBF010000208          514 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000208 DANKBF010000000
VERSION     DANKBF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..132)
                     /locus_tag="PWY89_003062"
     CDS             complement(<1..132)
                     /locus_tag="PWY89_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2068010.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DANKBF010000209          494 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000209 DANKBF010000000
VERSION     DANKBF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..325
                     /locus_tag="PWY89_003063"
     CDS             <1..325
                     /locus_tag="PWY89_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2068011.1"
                     /translation="KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQR
                     EGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
BASE COUNT          197 a           66 c           90 g          141 t
ORIGIN      
        1 aaaggcaagt ttaaaatcac taaaagatac atggcttgat ttatcagaag ataatccata
       61 cagccaattc ttaattactg taatggctgg tgttaaccaa ttagagcgag atcttattcg
      121 gatgagacaa cgtgaaggga ttgaattggc taagaaagaa ggaaagttta aaggtcgatt
      181 aaagaagtat cataaaaatc acgcaggaat gaattatgcg gtaaagctat ataaagaagg
      241 aaatatgact gtaaatcaaa tttgtgaaat tactaatgta tctagggctt cattatacag
      301 gaaattatca gaagtgaata attagccatt ctgtattccg ctaatgggca atatttttaa
      361 agaagaaaag gaaactataa aatattaaca gcctcctagc gatgccgaaa agccctttga
      421 taaaaaaaga atcatcatct taagaaattc ttagtcattt attatgtaaa tgcttataaa
      481 ttcggcccta taat
//
LOCUS       DANKBF010000210          492 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000210 DANKBF010000000
VERSION     DANKBF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
BASE COUNT          162 a           96 c           57 g          177 t
ORIGIN      
        1 atgctaaatc actctacaat aacggcgaaa gcacaaaaaa taaaaaaatt attacgttat
       61 taaaaaaaca gatgaaagat caggttttat tagatctcat tctttcatca cttgaaggga
      121 tttaatgaac tactttttcc tctttgtcgt ttttatatat tcgtattcct tcaaacagta
      181 ggatagcctc tttcaatgct tgatatttga actgtccgtt acttaaaaat ttagatgtgc
      241 cgccctcttc ctagaacata ttctttttta ctttttcatt aattgacaga ccaatcgaac
      301 gaaactgtaa atttcgatag gataatgatt ggtcaaactc attcattttc aagacatctc
      361 gattcatatt caagatagga atctttttat caacatcact ttattcgtat tttcctctac
      421 cataaaagct tcacgctcca caaaattgtt ttatacacta ctattcagcc cattcaacga
      481 ccaatgaatt tt
//
LOCUS       DANKBF010000211          468 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000211 DANKBF010000000
VERSION     DANKBF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..>468
                     /locus_tag="PWY89_003064"
     CDS             <1..>468
                     /locus_tag="PWY89_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2068012.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DANKBF010000212          462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000212 DANKBF010000000
VERSION     DANKBF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..430)
                     /locus_tag="PWY89_003065"
     CDS             complement(<1..430)
                     /locus_tag="PWY89_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2068013.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DANKBF010000213          460 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000213 DANKBF010000000
VERSION     DANKBF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(20..91)
                     /locus_tag="PWY89_003066"
     tRNA            complement(20..91)
                     /locus_tag="PWY89_003066"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="PWY89_003067"
     tRNA            complement(113..200)
                     /locus_tag="PWY89_003067"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="PWY89_003068"
     tRNA            complement(225..298)
                     /locus_tag="PWY89_003068"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="PWY89_003069"
     tRNA            complement(312..382)
                     /locus_tag="PWY89_003069"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="PWY89_003070"
     tRNA            complement(384..459)
                     /locus_tag="PWY89_003070"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DANKBF010000214          449 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000214 DANKBF010000000
VERSION     DANKBF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(34..270)
                     /locus_tag="PWY89_003071"
                     /pseudo
     CDS             complement(34..270)
                     /locus_tag="PWY89_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           76 c           77 g          150 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaataacgc cgtttgtata attaagcgag acaaataaaa
      301 aagccattta tgttacactc caattgtaat acccgctaaa gcatacaagg agagtgaaca
      361 taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc atattttcat
      421 caagaaactc cggttgctat cgttgcgaa
//
LOCUS       DANKBF010000215          437 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000215 DANKBF010000000
VERSION     DANKBF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            1..437
                     /locus_tag="PWY89_003072"
                     /pseudo
     CDS             1..437
                     /locus_tag="PWY89_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          179 a           77 c           80 g          101 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattga
//
LOCUS       DANKBF010000216          430 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000216 DANKBF010000000
VERSION     DANKBF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..>430
                     /locus_tag="PWY89_003073"
     CDS             <1..>430
                     /locus_tag="PWY89_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL2068014.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DANKBF010000217          421 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000217 DANKBF010000000
VERSION     DANKBF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(170..355)
                     /locus_tag="PWY89_003074"
     CDS             complement(170..355)
                     /locus_tag="PWY89_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HDL2068015.1"
                     /translation="MQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRA
                     WRKMLDDELIPLENELR"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DANKBF010000218          420 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000218 DANKBF010000000
VERSION     DANKBF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            74..403
                     /locus_tag="PWY89_003075"
     CDS             74..403
                     /locus_tag="PWY89_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2068016.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DANKBF010000219          415 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000219 DANKBF010000000
VERSION     DANKBF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..339)
                     /locus_tag="PWY89_003076"
     CDS             complement(<1..339)
                     /locus_tag="PWY89_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2068017.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DANKBF010000220          411 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000220 DANKBF010000000
VERSION     DANKBF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.255"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..>411
                     /locus_tag="PWY89_003077"
     rRNA            <1..>411
                     /locus_tag="PWY89_003077"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DANKBF010000221          404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000221 DANKBF010000000
VERSION     DANKBF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..>404
                     /locus_tag="PWY89_003078"
     CDS             <1..>404
                     /locus_tag="PWY89_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068018.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DANKBF010000222          402 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000222 DANKBF010000000
VERSION     DANKBF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(37..396)
                     /locus_tag="PWY89_003079"
     CDS             complement(37..396)
                     /locus_tag="PWY89_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068019.1"
                     /translation="MINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEV
                     IEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSK
                     IDKQIDDFPEEIIDRIE"
BASE COUNT          114 a           77 c           69 g          142 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aa
//
LOCUS       DANKBF010000223          381 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000223 DANKBF010000000
VERSION     DANKBF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.260"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(21..94)
                     /locus_tag="PWY89_003080"
     tRNA            complement(21..94)
                     /locus_tag="PWY89_003080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="PWY89_003081"
     tRNA            complement(108..181)
                     /locus_tag="PWY89_003081"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="PWY89_003082"
     tRNA            complement(209..281)
                     /locus_tag="PWY89_003082"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="PWY89_003083"
     tRNA            complement(301..374)
                     /locus_tag="PWY89_003083"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DANKBF010000224          376 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000224 DANKBF010000000
VERSION     DANKBF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..>376
                     /locus_tag="PWY89_003084"
     CDS             <1..>376
                     /locus_tag="PWY89_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2068020.1"
                     /translation="SKEIQDKILEEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKN
                     EGFAGVEELVPATVFERVFEDLEQSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLC
                     EAVKKVLDNGFDVINMKQFKLSG"
BASE COUNT          140 a           52 c           79 g          105 t
ORIGIN      
        1 tatctaagga aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat
       61 tagtattaat gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg
      121 aagtagtgaa aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg
      181 aacgtgtatt tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta
      241 acacaactgg tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta
      301 aactttgcga agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc
      361 agttcaagct atcagg
//
LOCUS       DANKBF010000225          373 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000225 DANKBF010000000
VERSION     DANKBF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
BASE COUNT          130 a           79 c           26 g          138 t
ORIGIN      
        1 tgtaagcaac aatgtatttt aagtgtaatg cttttatgca cctatttaat ttatcattct
       61 catctgatac aatctcactc ataacaatat gaatttttct tctcattagt ctcagttcac
      121 catgaattat acataacacc caatacaatt atttttccat acttttttaa gaggacaatt
      181 acatcgtata tatcgctaag aattgcaacc attccatttt taaagacata tttcctctaa
      241 aaaactaaaa ttctatctat ccacgactaa taatccccca cattaaaccc gtaatcgaac
      301 cagaaaattt ttctttataa ctaaaacaac catcattttc gagttatctt aaacacatat
      361 ttcctatttt ttc
//
LOCUS       DANKBF010000226          372 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000226 DANKBF010000000
VERSION     DANKBF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            82..>372
                     /locus_tag="PWY89_003085"
     CDS             82..>372
                     /locus_tag="PWY89_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2068021.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRT"
BASE COUNT          132 a           61 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cc
//
LOCUS       DANKBF010000227          369 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000227 DANKBF010000000
VERSION     DANKBF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            98..>369
                     /locus_tag="PWY89_003086"
     CDS             98..>369
                     /locus_tag="PWY89_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068022.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          139 a           52 c           70 g          108 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttt
//
LOCUS       DANKBF010000228          368 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000228 DANKBF010000000
VERSION     DANKBF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..272)
                     /locus_tag="PWY89_003087"
     CDS             complement(<1..272)
                     /locus_tag="PWY89_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068023.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DANKBF010000229          362 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000229 DANKBF010000000
VERSION     DANKBF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.267"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..>362)
                     /locus_tag="PWY89_003088"
     rRNA            complement(<1..>362)
                     /locus_tag="PWY89_003088"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DANKBF010000230          323 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000230 DANKBF010000000
VERSION     DANKBF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="PWY89_003089"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="PWY89_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL2068024.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DANKBF010000231          321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000231 DANKBF010000000
VERSION     DANKBF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.274"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..>321
                     /locus_tag="PWY89_003090"
     CDS             <1..>321
                     /locus_tag="PWY89_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2068025.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DANKBF010000232          315 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000232 DANKBF010000000
VERSION     DANKBF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.276"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..>315
                     /locus_tag="PWY89_003091"
     CDS             <1..>315
                     /locus_tag="PWY89_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068026.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DANKBF010000233          309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000233 DANKBF010000000
VERSION     DANKBF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.277"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            1..309
                     /locus_tag="PWY89_003092"
                     /pseudo
     CDS             1..309
                     /locus_tag="PWY89_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DANKBF010000234          302 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000234 DANKBF010000000
VERSION     DANKBF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.denovo.278"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
BASE COUNT          107 a           33 c           48 g          114 t
ORIGIN      
        1 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacattc
       61 taatatattc atatgagcta attttatgat taaatggtaa gtttttcctt gtttgtttta
      121 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga tctgtggggt aaatcattat
      181 acaaaagtaa aattttactt attaacaggc tgtagaaata taaaaaagaa ctaaaattta
      241 actaaattat gtgctattct taaggaaagc caagtgtatg agaaagtttc tgagaatata
      301 at
//
LOCUS       DANKBF010000235        23644 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.042, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000235 DANKBF010000000
VERSION     DANKBF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(23..604)
                     /locus_tag="PWY89_003093"
     CDS             complement(23..604)
                     /locus_tag="PWY89_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL2068027.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFIDYYLSN"
     gene            complement(791..1441)
                     /locus_tag="PWY89_003094"
     CDS             complement(791..1441)
                     /locus_tag="PWY89_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068028.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1984..2919)
                     /gene="whiA"
                     /locus_tag="PWY89_003095"
     CDS             complement(1984..2919)
                     /gene="whiA"
                     /locus_tag="PWY89_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2068029.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2953..3951)
                     /locus_tag="PWY89_003096"
     CDS             complement(2953..3951)
                     /locus_tag="PWY89_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2068030.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3948..4832)
                     /gene="rapZ"
                     /locus_tag="PWY89_003097"
     CDS             complement(3948..4832)
                     /gene="rapZ"
                     /locus_tag="PWY89_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2068031.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4967..5698)
                     /locus_tag="PWY89_003098"
     CDS             complement(4967..5698)
                     /locus_tag="PWY89_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2068032.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5702..6967)
                     /locus_tag="PWY89_003099"
     CDS             complement(5702..6967)
                     /locus_tag="PWY89_003099"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2068033.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7230..10046)
                     /gene="uvrA"
                     /locus_tag="PWY89_003100"
     CDS             complement(7230..10046)
                     /gene="uvrA"
                     /locus_tag="PWY89_003100"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2068034.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10056..12050)
                     /gene="uvrB"
                     /locus_tag="PWY89_003101"
     CDS             complement(10056..12050)
                     /gene="uvrB"
                     /locus_tag="PWY89_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2068035.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12309..13811
                     /locus_tag="PWY89_003102"
     CDS             12309..13811
                     /locus_tag="PWY89_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2068036.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13813..14550
                     /locus_tag="PWY89_003103"
     CDS             13813..14550
                     /locus_tag="PWY89_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2068037.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14721..15914)
                     /locus_tag="PWY89_003104"
     CDS             complement(14721..15914)
                     /locus_tag="PWY89_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2068038.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15993..16199)
                     /locus_tag="PWY89_003105"
     CDS             complement(15993..16199)
                     /locus_tag="PWY89_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2068039.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16192..17334)
                     /locus_tag="PWY89_003106"
     CDS             complement(16192..17334)
                     /locus_tag="PWY89_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2068040.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17846..18481
                     /locus_tag="PWY89_003107"
     CDS             17846..18481
                     /locus_tag="PWY89_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2068041.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18635..18712
                     /locus_tag="PWY89_003108"
                     /pseudo
     CDS             18635..18712
                     /locus_tag="PWY89_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18957..19073
                     /locus_tag="PWY89_003109"
     CDS             18957..19073
                     /locus_tag="PWY89_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL2068042.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19089..21008
                     /gene="tet(M)"
                     /locus_tag="PWY89_003110"
     CDS             19089..21008
                     /gene="tet(M)"
                     /locus_tag="PWY89_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDL2068043.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21127..21294
                     /locus_tag="PWY89_003111"
     CDS             21127..21294
                     /locus_tag="PWY89_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL2068044.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21354..21707)
                     /locus_tag="PWY89_003112"
     CDS             complement(21354..21707)
                     /locus_tag="PWY89_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2068045.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22211..22630
                     /locus_tag="PWY89_003113"
     CDS             22211..22630
                     /locus_tag="PWY89_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2068046.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22656..22862
                     /locus_tag="PWY89_003114"
     CDS             22656..22862
                     /locus_tag="PWY89_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2068047.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23090..23263)
                     /locus_tag="PWY89_003115"
     CDS             complement(23090..23263)
                     /locus_tag="PWY89_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068048.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23239..>23644
                     /locus_tag="PWY89_003116"
     CDS             23239..>23644
                     /locus_tag="PWY89_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HDL2068049.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPA"
BASE COUNT         7054 a         4733 c         4211 g         7646 t
ORIGIN      
        1 taaatggaaa gttttgtctc gcttaatttg acaaataata atctataaac atcaccccaa
       61 ataaaacaga aaacagtata agcattataa aaattatcaa caaccttgat ccttctttta
      121 tagattcagg aaacagtata attttagtac tagataaatt gtttacaccg attagtgtta
      181 aaatatcttt attcttcttg ctaacataac cctttatgca caaaagaaca gctatactta
      241 aagtaacaaa taaataacgt attccaatag aaaaatcact ataaatactc tctagcatag
      301 gaccgacaat tgaagaagtc actactgcaa atgctggacc tatccaagca acattagaat
      361 gttctttctc tcttttcctt tgtaaatctt tgttgagtat taatctattt atttcttctg
      421 caggcaagtt aattttcata accttatggg ggattatcca attaaaaatc ggaaaaaggt
      481 agcctcccca aaaagtatag tgatctgtgt caatcagata ataacctgat tttgattcca
      541 gaataacata tctcaaagcc gaacgaaaat ttttcatgaa tatccataat ctcactgttt
      601 ccacacctaa ttcctccaat tccaattaaa tggatttata gcatcaccaa ttatgttaac
      661 tgcctgacca acctcattga taaaattggc accactatcc catgcttcaa caatgctata
      721 tatctatcaa acaaaaaatg gaaaactaaa tactcgcgca cataaatttt acattctgtt
      781 ttttataaga ctaataataa tcattgaaaa aatttcttat cggtggcgca tattcccaat
      841 tgattaatgt gatcccatat tctgcaagta ttttacgagc ttcttctaag tcatagtgag
      901 aaaaatgcat aaaacctgca atacgatcac ggactgtgag ctgtggcttg cttccaccat
      961 cattcataaa atatccagca gagccactac ttaaagtgat tgttacatct tcactttcta
     1021 ttattaagaa ttcataatga aatgctttca actctccact attatttaaa agttttatac
     1081 tccaacttcg tctaggttga gttgtcacat tccctctaaa acttcctata aattctgcaa
     1141 tcaccgtatc ttcaatcaaa attctttcct catctatttc atattcaaga ctaaacggaa
     1201 attctccata tgtaatctct ggcaatggtg gtgctgtagt tctccaaata aatacaataa
     1261 ctgtgaatag aaaaaatcca acgatcgata taaatgttat gagacctgct cgaagtaaaa
     1321 cccgatataa taaaaatgct gtatttcgct ttgctatatc tagaaaaata aaaagtactg
     1381 caatcataat gatagtaaaa gttgtaaaaa agcacatttt ttgccctccc tttataaaca
     1441 tcgattctca aattctaaac caaatcttga tagttcaagt tcaggtataa cgtataccaa
     1501 ccatacatcc caattactat ttatgaactt atgattatca ctgaataaat cactccatcc
     1561 ccttgttaaa acttctacat taattacacc tggttgtttt cgactagcta atccaatgaa
     1621 cttatcaacc atccttatcc accaaccacg agaatcttga ccagaagtaa gaaaaacagt
     1681 tctcaaggtt gatcgagcag ttccttccag tttttaatta atctccagtt atagataaat
     1741 acttgatcct atctatatca ggacttctat attaagcaaa agtttatgta taaaactaaa
     1801 atcttcattc agttgtctcc ccgcttttta tacgattgta ttaaaaaaca atagaagcct
     1861 ttcaacatta tgttactcca gtaaagttgg aattatagtt tgacggaaga aaagcaagta
     1921 tagcttttaa aaaaaactga tacattcgca tctcctgctt catgtatcag ttttttcttt
     1981 ggattaaact gcttcttcac gcagtttttc cgcaaattca tttattttgc gaattcggtg
     2041 attaattccc gattttgata ttgcacctga gggaatcatt tcccctaatt ctttcaagct
     2101 gacctctgga tgttgaagcc gaagttctgc gatttcctga agtttttcag gaagggcatg
     2161 tagaccgacc tttgcttcga tcaattcgat gttttcgatt tgtttagaag ccgcatcaat
     2221 cgtcttgttc agatttgctg tctcacaatt gactagacga ttgaccgaat ttctcatatc
     2281 ccgtacgatt cgaacatctt caaatcggag cattgagttg gttgcaccaa tcaatgttaa
     2341 gaaatcagca attttttctg ctccttttaa atacgaaata tagccattgc gccgttcaag
     2401 cgttcgagca tgtagaccgt aataatttaa catatcgcaa atgtcttggt tatgctgttc
     2461 ataaatagaa aatatttcca aatgataacg actcgtttcc ggattattga ctgaacctga
     2521 ggccataaaa gcaccacgta agtaagaacg cattttttgc gaatttccca taatctcgtc
     2581 cgaaacgtgt ccattgaaca tcataccatc catgatatca agatccatca acacatcttt
     2641 tgtgccttgc ttcaaccgga cgatatagac attgttcttt tttaatttca tttttttacg
     2701 aacgagtagc tcacttcgta cctgataatg atctttcaaa agagaataga tacgtctggc
     2761 aattgcagca ttttctgttt ggacgttcaa aataaactgc tggttcacaa tacctaaaga
     2821 gccgttcatt cgcagcaaag cagctaattc cgctttcgca tgttctcgat gaacttccaa
     2881 accagttagt tctttcttca catctgcagc aaatgacatc ctattctcct cctttctcca
     2941 acaataaatt gcttaatatt ttgaaccgaa aactaaacgg aacaattctt ctactacttt
     3001 gtctccatcg tggaagacac caccatcttt caattctaaa aagtcggtgg aaatcactcg
     3061 acatccttca tttctcaatc cgttgaagtc gtgtgcgact tgtactagat actcatcata
     3121 gatctctggg tccatataat cttcaggcac tttttccgta ttgaccaata ctgtatcgat
     3181 aaaaggctga ttcaaatgct tgtgtaaaac acgtatatga tcggcatctg tgaaatgttc
     3241 cgtttcccct ttttgggtca tgatattaca gatatagacg gtttcagcag ttgtcttgag
     3301 cattgcttcg ccgatttctg gtatcactaa atttggaagg atactggtga ataaacttcc
     3361 gggtcccaaa acgaccatgt cggcgtccat aattgctgaa acgacttttc gagccgcttt
     3421 ggcttcactt ttatcatttg tgttgcagac atacacacgg tcaatggttt tacggttgat
     3481 cgcgatttta gattctccta ctgcagtcgt tccgtcttta aaaacagcat gcaaggtcaa
     3541 aggagtttct gaagaaggat aaacatgtcc atctacatgc atcatttttg ctaataattg
     3601 aatcgcctca taagtgcttc cccgcatctc ggaaactgcg gcaatgatca gattaccgat
     3661 cgtatgattg gctaaaaact gatcttcctt tttgaaacgg tattggaaaa tatcttcata
     3721 gaattgtggc atatccgata acgccactaa gacatttctc aagtcaccag gaggagccat
     3781 agcgatcgat tctctgattg caccgctgct tccaccatca tctgcaacag tcactactgc
     3841 tgtgatatct gctccttgat ttcgtaaact tttcaggata actggcagac cagtgccacc
     3901 gccgaccact acgatctttg gttttcttat ccggtaggtt ttcattttca cgaccggttc
     3961 accgtctctt tccttttatc cttgtcccga tgtgtcacat tgactttata gtttttccct
     4021 aaagcatttg ccaaacgttc agtcaatgcc acagaacggt gttgaccgcc tgtacagccg
     4081 attgcaactg tcaagctgct ttttccttct ttaatgtatc caggcaatac agtttccaga
     4141 agatccaaaa atttatggta gaatgattgc gtttcatcaa aggacatcac ataatcatag
     4201 actggctgat cttttccggt caatggtctt agttccggga tataatgagg gttgggcaaa
     4261 aaacgcacat ccatcacaat atccgaatcg atcggcaacc cgtatttaaa accaaatgat
     4321 acaaattcga tccgaaatcc ttggtctccc tttgatttga atgactcatt gattttttca
     4381 cgtaattgtc gtggtgtcaa tgttgtcgta tcaatgatca cggaagcttg ggctttcaag
     4441 tcgtctagta tcgcccgttc tttacgtatc ccttcagtga tcaaaccgtc catcgccatt
     4501 ggatgtgctc gtcttgtttc tttgtagcga gccactagtt cttcgtctga agcatctaaa
     4561 aataagatag aagtgtcgat gaagttcgta ttttcgattt cgatcagcat gttttggatt
     4621 tcttcaaaga aggaacgaga acgcaaatcc actcctaaag ccatttttgt gactttacct
     4681 gattctttga tcaattccca gaattttgga atcaatcgag gcggcatatt atcgatacaa
     4741 aaatagccca tatcttcaaa actttggata gctactgttt tccctgcacc actcatacct
     4801 gttatgatga ctaattgcaa attatctgtc atatttacga acccctctca cattgtgcat
     4861 aataaaatta tatcatcccg ggcgtatcat taccagtttt ctttttcttt tacaaaaaga
     4921 tttggaccaa atatctttta ggatatttgc cccaaatcca atttttttac ttttccgatg
     4981 ctgtatccaa tgcctcgctt ctttcagcta atgcacgttc gatcgttcga tccgctaaaa
     5041 tagattgagc atcgatcact gggtaatgat tatcttcagc aaattcatgc atcaaagata
     5101 attctgtgca acctaggatg actttttcac agtttaggcg ctctaccgct tcctctaaga
     5161 tttcgtaata gagctcttga ttcaaataat ccgattcctt gatctcatga tagatcaggt
     5221 aattgatttt ttcttgaagc gcagtatcag gaatcactgt ttcgaaacca agatttttga
     5281 cttcgcgttc atagattcca gctttcatac ttccttctgt tcctagaatc gctactctcc
     5341 ctgtcgtatg ctgacggaca agttcatttg ctgcttctct tggcatatgc aagataggaa
     5401 tatccgttgc cgcttgtaac tcttcaaaaa agtagtgtgc ggtgttacaa gtcaacacaa
     5461 tgaagtttgg tcgcaataaa ttttgtttct caatatcatc cagcagaaac ggcattggat
     5521 tttcttctga tcgatctaaa atataagctg tacgatcagg aactgttgca tgattgaata
     5581 agacataatt caaatattct tgatcttttg tagctttcgt tcggtggttg atcaagcgaa
     5641 caaaactttc tgtcgccatc gtgcccattc cgcctaaaat actgaaaaaa ttctccatga
     5701 actagccttc tttcacatga aaatactttt tgaatcttgg aatatagtta tggaacatat
     5761 atttcatcaa caaccagcgc ttgattgatc ggtctttttc ataaaataca gtagtaccgt
     5821 aacgattttc tttgatcatt tgctcagcta gttttttgtc ttcattttcc cgagcatatt
     5881 ctaaaaagat ttttttgggc acacccatcc ataatcttgc tttatctgat tgattcgttc
     5941 cataggtcgt ttcaacaaag ggtttgttga acactcgatc ttctgtgaca aaacgagcca
     6001 gattcaagcc gttcaatgtc acaaagaaac tgctacgacc ttgtctcaaa ttgatttcaa
     6061 ataatttata ttctccatct ctaggatcat acttcatatc gaaattggca aagcctgtat
     6121 attcgatttt ttccaaaaat gccttaatgg tttgatagat tttttcgtta taatctggca
     6181 tgatcacaac gtaattacca atcgatgctg gtgtaggatc ttctaatagt ggatgaccaa
     6241 ggcacatcat ccgaacttga tgatcttcat ctacatacgc atttagcaca cgcatattac
     6301 tatcatcacc cgggataaaa tcttgtacaa tcatttcact tttataaccg gcttcgtata
     6361 tccggcctaa aattaaatcg aactcttctc tagtctctaa aataaaagct ttttttcttc
     6421 cttcaaattg gacagaaaga tactccacac tgtttgcagg tttcaaagca actgggaatt
     6481 caaaaggcaa ttcctgttcc aaatgtccat ttactaacat ttcttctctg acgatcaatg
     6541 ttttaggata cggaagatca tattcttcac aaacttcata aaagcttact ttattgatca
     6601 gcctttcaaa taaagaataa tcaatatatg gacagataaa atattctgac agctcttgtt
     6661 tatgttggga gatcaattct gcatatccat caccacaagc aatcaaaaga tattttttac
     6721 tcttatcaga atatctttct tttgccaaac gaagcatcgt ctcgataaat acagggtctt
     6781 tatcaaatcc tggaataact tctacattga caattttact gtagcgggta ggggctaact
     6841 gatccgaagc atatgcttgg cagattttgc cgtatgcttc attaaaagaa cgtgccatac
     6901 cataaacgtt catgtcgctt cctaataata tagggataaa ttcttcattt tcaatactgt
     6961 tcatcattat ctctcactta ctcataatct cttgtttttc ttttccaact acacaaggaa
     7021 tatcatacca taaagaaaaa agcaatcaaa ggaaagtcct ccgattgctc aattcttttt
     7081 tgaacggatt gaacttatag gaagttttta tctataccaa tcagtgtaga aagaactacc
     7141 atgtaatgat tctagaacac tgatattaat aaaacaagca aaaattcatt tttttatgtt
     7201 tttttaacta tctcagctat ctatcctctt tataccatta tttttttcaa atagtgacct
     7261 gtataacttt cttttacgtt aatgatttct tctggtgttc cagtcgcaag gatcgttcct
     7321 ccaccttctc caccttctgg tcctaaatca atgatataat ctgctgtttt gattacatct
     7381 agattgtgtt caatcactaa aactgtgtta ccagcatcta ctaatctttg taatacatgc
     7441 aacaagcggg cgatgtcatc gctatgaagt cctgtcgttg gttcatctag tatatagaaa
     7501 ttctttccat tagagatttt gtgcaattca ctggcaagtt tcatccgttg tgcctcacca
     7561 ccggacaatg tcgttgctgg ttgccccatc gtcacataac ctaagccaac atcaacaatc
     7621 gtttgcagtt tgcgatgaat ctttggaatg tgcttgaaga attctacagc atcctctact
     7681 gtcatttcca aaatatcagc aatgcttttt cctttgtaat gcacttctaa tgtttcagaa
     7741 ttatatcgtt taccatgaca tacttcacaa ggaacataga catcaggcaa aaagtgcatt
     7801 tcgatcttaa taattccatc cccgcgacaa gcttcacaac gaccgccttt tacgttgaaa
     7861 ctaaaacgcc ctttcttata accccgcatt tttgcctcgt tcgtttgagc aaataaccca
     7921 cggatatcat caaatacact tgtataagtc gctggattac ttctcggcgt ccggccgatt
     7981 gggctttgat cgatatcgat gatcttttcg atactttcgt agccggaaat tgtcttgaat
     8041 ttacctggct tagcagaatt cttatttaat ttttgcgcta acgatttctt taagatacta
     8101 ttgaccaatg tactctttcc agaaccagac acacctgtga ctgctacaaa ttctcctaat
     8161 ggaaattcaa cactaatatt tttcagattg ttttctgaag caccagtgat cttgattgct
     8221 ttcccattac ctttacggcg ttcttttggt acagggattg attttttccc tgaaagatat
     8281 ttccctgtca aagaattttc atcattagct acttcttcag gagtgcctgc agccacgatt
     8341 tgtccgcctt gttctccggc cccaggtccg acgtcaatca aataatctgc tgcccacatc
     8401 gtatcttcat catgttcgac tacaacgagg gtgttgccaa gatcacgcat tttctttaat
     8461 gagccaatca gtcgatcgtt gtcgcgttga tgcaatccga tcgatggttc atctaaaata
     8521 tacaaaactc cagaaagatt cgatccaatt tgtgtagcca atctgattcg ctgcgcttct
     8581 cccccagata atgttccgga agcgcgactc aaagttagat agtctagtcc cacattttcc
     8641 aagaatgaaa gtctgtcatt tacttctttt aaaatcggtt tggcaattac tttctcttgt
     8701 tcagataacg ttaagtttga gaagaaatct actgcttttt gaatagacaa ctcatttgtt
     8761 tgcccgatat tcgttccatt gatttgaaca gatagagctt ggggattcaa tcgataacct
     8821 ttacatgtct gacaagttaa ttctgtcata taaagacgca tttgctcacg cgtgaaatca
     8881 ctgttcgtat catgataacg acgctcgata ttcttcaata ccccttcgaa agtcgtttct
     8941 acatcgcgaa cgccaccaaa atcattttca taatggaaat ggaaaagatc atctgtccca
     9001 ttcaagatga tctcctgatg ctcttccggt aattcttcga atggtgtatc taaatcgata
     9061 ccgaaacttt cacaagcctg cgctaacatt tgaggataat actgagagct gattggattc
     9121 catggaacga tagcaccttg actaagtgtc ttcgttctat ctggaatcac taaatcttga
     9181 tcgacttcta atttgacacc taaaccatca cagtctggac aagctccaaa aggcgcattg
     9241 aatgagaaca gtcttggttc tagttcgcca acagtaaatc cgcaataagg acaggcatag
     9301 tgttcgctaa acaacatttc ttcttcaccg atcacgtcaa ccagagcata gccgtctgct
     9361 aatcgcaggg ccgcctctaa agaatcaaat aggcgagaac gaatcccttc tttcacaatg
     9421 atccggtcga tgacgatcgc gatatcatgt tttttgtttt tctccagctc aggcacttca
     9481 cttaaatcat agatctcacc atcgactcga acgcggacgt atccctcttt ttggattcgt
     9541 tcgaatattt ttttatgttg tccttttttc tttaccacga tcggtgccaa cacttggatc
     9601 tttgtgcgct caggcaaagc cattacttga tctgccattt gatctactga ttgactggta
     9661 atctcgatat gatcgttggg acaaatcgga tgtcctacac gtgcaaacaa caagcgtaaa
     9721 tagtcattga tttccgtcac agtacctact gtcgaccgag gatttttact tgttgttttc
     9781 tgatcaatag aaatcgccgg actcaatccg tcgatgctat ctacgtccgg cttatccatt
     9841 tgtcctaaaa actgacgagc ataagcagaa aggctctcta cgtatctccg ctgcccttca
     9901 gcgtacagcg tatcaaatgc tagggaactt tttccagaac cggacaaacc tgtaacgacc
     9961 acgaatttat ctcgtggaat cgttacatct atatttttca agttatgggc acgtgcacca
    10021 tgaatcgtaa ttttatcgtt agccatgttg tcctcctact ttgaagcttt taattctaaa
    10081 atcatatctc gcacattcgc ggctgtttca aaatccaaag cttttgccgc atctttcatt
    10141 tctttttcta atttcatcaa taacgtgtct ttttcttcct tcgtcatttc ttcgtacgag
    10201 acttgtaccg cttcttttgt atcatcttcc gattctttcg taatagaaat caaatctcgg
    10261 atttctttaa tgatcgtttt tggaacgatc ccatgttctt cattgtactt ctgctgaatc
    10321 gtacgacgtc ttgacgtttc atccatcgct agacgcatag aatctgttac tttgtctgcg
    10381 tacatgatta ctttaccttc ttcattccga gcagctcgtc caatcgtttg gactaaggaa
    10441 cgttcactac gtaaaaagcc ttctttatcc gcatccaaga tagcaacaag ggaaacttca
    10501 ggtacgtcta atccttcacg aagcaaattg attccaacta acacatcaaa ttcacctaat
    10561 cgaagatctc gaatgatttc tgttcgttcc agcgttttga tatcactatg caaatatttg
    10621 actttgatcc ctaattcttt gaaataatct gtgaggtctt ctgccatttt tttcgtcaat
    10681 gtcgttacaa acacccgctg gtctttttcg actcgttcat ttatttcgcc tactagatca
    10741 tcgatttgtc ccatgattgg acgaacttcg atcactgggt caagcagccc tgtcggacgg
    10801 atgatttgtt ggataacggt atctgtttgt tcatgttcat atggtcctgg cgtcgcagag
    10861 acataaatga tctgattgac atgtttttca aactcttcta accgaagtgg ccgattgtct
    10921 aaagcagatg gcaaacggaa accgtaatct actagcatct gttttctcgc tcggtcacca
    10981 ttatacattc ctctgatttg aggcatggtt acgtgcgatt cgtccgccac gatcaaaaaa
    11041 tcttcaggaa agaaatccaa tagcgtataa ggcggttcac cttctttacg tccgtccata
    11101 tgccgagaat aattttcaat tcccgacgtg tagcccattt ctaacatcat ttcaatatcg
    11161 taattggttc gctgttctaa acgttgagct tccagaagtt tgttttcatt tcgcaagaca
    11221 gttaaacgct gttctaattc ctctttgata ttcgctacgg catgctccat atgttcatca
    11281 ttcgtcacaa agtgagtcgc cgggaagatt gctacgtgtt ccgtttctcc taaaacttca
    11341 cctgtcagtg cattgacttc tcggatacgt tcgacctcat ctccaaaaaa ctccacacgt
    11401 aaggcacgtt catctcttga agccggaaaa atctccacga catcaccgcg cactcgaaaa
    11461 cgaccgcgct gaaaatctat atcatttctt tcgaattgga tactgactaa gtcacgaatc
    11521 aactgattgc gatctagttc tgctccctgt cgaatcgaaa ctacttgttt ttggtactca
    11581 aaaggcgagc ctaatccaaa aatacatgaa acggatgcaa tgacgatcac atcatttctt
    11641 tctaataatg aactagtagc cgaatgtcgt aatttgtcga tctcatcatt tacacttgaa
    11701 tctttttcga tatacgtatc gcttgaaggc acataagctt caggctgata ataatcataa
    11761 taagaaacaa aatattcaac agcattgtta gggaaaaatt ccttgaattc cccgtacagc
    11821 tgaccagcca atgttttgtt atgggcaata atcagtgttg gtttattcac tttctcaata
    11881 agattggaaa tcgtatatgt tttccctgtt ccagtcgcac ctaataagat ttgtgctttt
    11941 ttcccgccga ctacgccatc cactaattgg ttaatcgctt caggctgatc accagccggt
    12001 tggtatttcg agacaagctc aaagtgtcgg gaggtatctc tctcaatcat tcttcgtcct
    12061 ccttttcatt cattcctttt cattttacga ccaataaaaa gaaaaggaaa gcaaaggcat
    12121 tccttactaa aaaattatac cacaccgaac atactttcgc cactttttta tcttattata
    12181 catgttatgt tttataacat aaaatgttag aaactcgcat ttttttcatt ttttcgagct
    12241 tacagtcttg tctgattcag cacttttcgc ctataataat aaaatattgg aaatttagga
    12301 ggttgattat gaaaagaaag catttatttc actcatttgt tttcttaatg acaggacttg
    12361 tgacacttgc gtttggtcat gttgcacaag ctgacaacac tacaacggca gagtcaagca
    12421 caagcgagac aatgcaaaaa atagagccca aaaaagacgt atacgtaatt gccagtgact
    12481 ctgcttttgc tccatttgaa tttcaaaacg caaagggcga gtacgaaggc atcgatgtaa
    12541 aattgatgga aaacatcgcc aaactgcaag gatttaacat ccgaatggac taccgtggtt
    12601 tctctgctgc tttgcaagct ttggaatcag gacaagcaga cggaatgatt gccggaatga
    12661 ctgtaacaga tgaacgaaag aaaagctatg atttttctga ttcttacttt gatagcggga
    12721 tccaattagc tgtcaaaaaa ggagatactt cgatcaagtc ttatgaagat ttgaaaggaa
    12781 aaacagtcgg agcaaaagtt ggtaccgaaa gtgctgaatt tttaagcgac catgaatctg
    12841 aatatggcta ttctatcaaa tattttgatg ctgctgatca attgtacgac gggttaaaag
    12901 ttggcgcaat cgatgcaatg atggatgatt atccggtaat cggttatgcg atcaaacaag
    12961 gacaagaatt ggaaacaccg atcaaaaaag aatctggcgg aaaatatgca tttgccgtta
    13021 aaaaaggaca aaatcctgag cttcttaata tgttcaatga aggattgaaa gaattaaaaa
    13081 gaacaggcga atacgataag attattaaag aatatgtagc ggatggttca gaaacttcca
    13141 gtgagtcttc cactgctgat gaatcaacat ttcttggact tatccaaaac aactatcaag
    13201 cattgttgaa aggactttgg agaacaattc tcctaacttt gatctccttt gctttggcta
    13261 tagtaatcgg tgtgattttt ggattattca gtgtagcacc aatacgcgct cttcgcacaa
    13321 ttgctagtat ttatgtcgat atcatccgag gaattccaat gatggttctt gcgttcttta
    13381 ttttctttgg gttgcctggt atcgttggat ttacgattcc tgacttcctt gctggtgtta
    13441 tcaccttgac tcttaacgca agtgcttata tcgcagagat tgttcgaggc gggatcaacg
    13501 ccgttcctgt cggacaaatg gaagcttcca gaagtttagg tctttcttat aatcggacaa
    13561 tgcaaaaaat cattttgccg caagcgatac gcatcatgat cccatcattc gtcaatcagt
    13621 tcgttatctc attaaaagac acaacgatca tttccgcaat cggtgtagtc gaattgctgc
    13681 aaactggtaa gatcattgtt gcacgtacaa cacaaagtac gtatgtttac ttgatcattg
    13741 cgatcatgta cttgattttg atcacagcat tgacaaaatt agcaaaagtt ttagaaaaga
    13801 aggtgaagta agatggcaga aaaaatctta gttgagcatt tagtcaaaaa atacggcgat
    13861 aacacagtat taaacgacat caatgtatcc atcaacgaag gagatgtcgt ctgtatcatt
    13921 ggcccttcag gttcaggaaa aagtacattc ttgcgttgct tgaatcgttt agaagaacct
    13981 tctgacggtg aaattattat tgacggcgcc cacttgatgg ataaaagcac agatatcaac
    14041 ttggtgagac aacatatcgg aatggtattc caacacttta acctattccc tcatctttct
    14101 attttggaaa acatcatttt agcaccgatg gacctgaaga aagaatctag agaagaagca
    14161 gagaaaaaag caatcaaatt actgcagaca gttggtctag aagataaaaa agatatgtat
    14221 cctgaaatgc tttctggtgg acaaaaacag cgtgtggcta ttgctcgtgc tctagcgatg
    14281 aaccctgaca tcatgctttt tgatgagcct acttctgcac tcgatcctga aatggtcggt
    14341 gatgtcttga acgtcatgaa aaagttagca gaacaaggta tgacgatggt tatcgttacg
    14401 cacgaaatgg ggttcgctaa agaagtggcc aatcgcgtca tgttcattga cgacggaaac
    14461 tttttagaag atggaacgcc tcaacaaatc tttgaaaatc cgcaaaatga acggacaaaa
    14521 gatttcttga ataaagtatt aaatatttaa aataaattat atatgtcttt ttgactatct
    14581 ttttatatct tcttaaatgt ccgcaaagcc ttattttata ggctctacgg gcattttgct
    14641 tttgtggtaa acctcacata tctaggtcta tcttcttata ttttcactat caagcgtggt
    14701 taaaatcgtg gtaaatactt ctatgcaatt agacgctgaa cctctgattt tgcggtatct
    14761 atggacgcat gagcatacca gttcattgtg atactgatat ttgaatgccc catgatatac
    14821 tgtaaatctt ttgggttcat gttcttgctt gccagtcttg tgcagaatgt atggcgtagc
    14881 gtatgcggtg tgatatgtgg caatggattg tccttgtggt gcttgttgta tttctttacc
    14941 atacggataa ataaggcgtt ataatcaatc gcaactttgg gcttgccctt atgattgaca
    15001 aataggaagt tgccccgtcc gtctatcaca aatggttctg cctttggacg ttttttcata
    15061 acctgttgaa atgcctgtat cgtttctttg cttaatggta cttgccttat tccgcttttt
    15121 gttttaggcg tttcaatata atagccctgt tccttgctct ttagtaactg gtggtcgata
    15181 atcacaactt cattcttgaa atcaatgtcg gctactgtca gtccgcacag ttccgaaata
    15241 cgaagtcctg tctttaacag tatcagcaca tcatcataat gcttgtgata cacattgtcc
    15301 gtcttgatga atgagagtaa ggcttgttcc tgttcctctg tcaatgcgac tttctttttg
    15361 gtatcatttt ctaggacttc actcaacttg aaatcaaagg gatttttcct cacataatca
    15421 tcttgaatgg ctatgtagaa tgacgctttt aacgagcgtt tatggttgtt aatggtattg
    15481 taggaaaagc ctttgtcttt catgcgtaac gcccattcct tagcgtcaga gggtttgatt
    15541 gtatcaatgc tcctagcacc taacttgtcc tctttcaata accgcatgag ttgttcccgt
    15601 tgtttctgtg tgcttttctt cacatttgcc ctctgtgcgt tctgtttggc ataaagttgg
    15661 caaagcgtca ttttcttgcc agtgctgtcg ataccgtcct caatatcccg tcttatctgc
    15721 tgttccagtt ctcgaagtga gggtttttcc cgttttccct ttggtgtcgg gtctgtgggt
    15781 gtcaatctcc aagcatacac atattttgtg tttccaaatg cgtctacata tttatataag
    15841 tattttctgt ctgttcgttg gctctctcca gtatgcagga tacgaccttt gttatcccgt
    15901 cttttttctt tcatggtgtc tgctcctttc cttgttggaa agagccttga tatgacttgt
    15961 gttcatcata acacatacaa ggctcatttg cattagatag cgtccaattt atcaatgacc
    16021 tgttcaaact gtcggcgttt aatctgtatg cggttgccat tcataatgag ccagccagcg
    16081 tccttgtttt cctctgccaa tcgtcttaac ttgttttctc cgatacggaa atactttgac
    16141 gcttcttcaa tggtaagggt gtatttttcc catacgggaa tatcctgtgc attactcata
    16201 aaacaccccc tcactttcga ttacgtctgc tatgtccgta cacatctgtt ccacaagcat
    16261 ttcctgcttt tcagagaggt gggtacattc atcaatcatt ttcatgtagt ttgagcctgt
    16321 cagcctgtcg atttcctcta tcatcacgat tgacggcatg acctgtcttt ccagccattg
    16381 cagagtgcgt tccatattga caggtacggg gttcatttca aaaggtaaac tgtcatggga
    16441 aatgaatatc tcccacagcg gatagtcggg aaattgcaca aaatcagtaa taaggtaaaa
    16501 caccagccca tgagggttat atgtcaataa caattcctca actgcctgta ctgcttttgt
    16561 accacgcaga cgaatttcaa aacgattgat aatatccgta tgtttctttt tggttgcctg
    16621 ttccttttgt ttttcataca ggcagaagta ttttgtgctt gcctttgaac cgatataaag
    16681 ggtgcttgct aaattacggt tttttccgct tagttttcca ccctgcacat tttcataatt
    16741 tttacagcga caatccgcac cgccatttct gtatttttca gacaaaacgg gaatatccaa
    16801 cagaccacac atatcattga ttgccaagtc aaaacgtcgg ataacaccgt cacagtcaag
    16861 acagcggttt aaaaatgaat accaatctct gttctgtgcc tgcaagacat attccatgtt
    16921 gcgtgagcct gcccctttta attccaagaa tacgcccatg tgttcgtcat cagaagccat
    16981 gaccttgatt tctccgtagg cgtaatgctc tttatagcca taatacccat agtcataatg
    17041 gataaaataa tctgctttca tgccaagcac ctttcggata atttccaatg cgtcagtcgt
    17101 tggataacgg acagacagat aatcaacgag cagataaaaa ggcttatcgc agatataata
    17161 attcagacaa cgcaggataa tattttgtgt ttccttgctg gcgttggctt ttccgttctc
    17221 aaagcggttg atactatatc ttgatacatg ggtaagactt gcaagttctc cttgtgaaag
    17281 accgcttgca agacgtttgt tcttcatttc aagtgcaaat tctaaattgt tcattgtatt
    17341 cctccaatcc cacaatagcc ttgaaatacc tgtaaacctc tattcaaaaa gtgtgatatg
    17401 cactatcttc ttaaaacttc tcattactac tcacaacttt gggattgtat ggtttttgac
    17461 ttgtcttttg tgaaatagtg cccccctgtt agataacggg ggcacagaca aggctcgcag
    17521 gctcgccagc cgacaaagca tttcttcccc ttgacacaag gtgtcatggc ggtaagcctg
    17581 agaggcttcc gccaccgtgt cagaaatact ttgtcagcaa gttctgtttt gccttttcta
    17641 acaagttcca tttcaaccga tattcatatc tacggattta atcagatagg tatttctttt
    17701 tgaatgtttt tatatttcat tctattgtgc ttattcgatt acaagaacat agtaattctt
    17761 atatgcttat ttgtcaatat ctttttttgc ctttacaaat catattaagc atgatagact
    17821 tataatagcg aagatataac gagatatgga gggttatgca atgaaaataa gccatttagg
    17881 aaataatata cagaccataa gaaaatttag gggaatgaaa caacaggaac ttgcggacaa
    17941 aatcggtatc aatatgcaga gcctttccaa gattgaaaga gggttgaact atcctgccta
    18001 tgaaacgctg gaaaagataa tggaagtgct ggacgttacg cccaatgaat tgttgtcggg
    18061 agaatggaag tatgtcaatc aatctgaaaa ggaagtctgt cagtttttaa gagccgaaga
    18121 acgtctaaat gcagaactga aacatggaca ctatgataac ttctttgaca gcgaggaaga
    18181 atggctggaa tatgagttag aaaagttacg ggaatatatt acagactata tcaacgggaa
    18241 aagcattgaa gcgtccgacc tttacccaat caaagaattt atccagcatt tgaagtttca
    18301 aaaattatta gaccgctatg atgatttata cagtatggat atgtttgggg aaagcataga
    18361 gggacataag tataagacac cttatcaagt cgtgaaaatg ataaacccga actcaaaaga
    18421 agatatggaa ctgttacgag aagtattaag gaataacaat tttgatgatg aggacgagta
    18481 acttttttcc tcttgcgaga agatacagca aacaaaaagc ccgatcaaga gtgcaaagca
    18541 ccaccaatgg tggcaaagct cttgacaggg ctttttcttt tctgttgtgg gcaatcaaga
    18601 ggaagaaagg gataaaatct gatttgtggt aaatcaggca aaggcaacgc aggtatctca
    18661 atttgatttg gtacttgaaa agaacgggag taattggaag attatagaat aacaaatatt
    18721 ggtacattat tacagctatt ttgtaatcac gtactctctt tgataaaaaa ttggagattc
    18781 ctttacaaat atgctcttac gtgctattat ttaagtatct atttaaaagg agttaataaa
    18841 tatgcggcaa ggtattctta aataaactgt caatttgata gtgggaacaa ataattggat
    18901 gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga
    18961 ttctaaagta tccggagaat atctgtatgc tttgtatgcc tatggttatg cataaaaatc
    19021 ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt tgaatggagg
    19081 aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca ggaaaaacta
    19141 ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga agcgtggaca
    19201 aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt acaattcaga
    19261 caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac acgccaggac
    19321 atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg gcaattctac
    19381 tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat gcacttagga
    19441 aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga attgatttat
    19501 caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc aaacagaagg
    19561 tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa tgggatacgg
    19621 taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca ttagaagcat
    19681 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
    19741 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
    19801 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
    19861 atacaaaaaa aagacaacgt cttgcatata tacgccttta tagtggagta ctacatttac
    19921 gagattcggt tagagtatca gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa
    19981 taaatggtga attatgtaag attgatagag cttattctgg agaaattgtt attttgcaaa
    20041 atgagttttt gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa
    20101 agattgaaaa tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga
    20161 gagaaatgtt gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt
    20221 acgtggattc tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag
    20281 tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaactaaaa gagcctacag
    20341 tcatttatat ggagagaccg ttaaaaaatg cagaatatac cattcacatc gaagtgccgc
    20401 caaatccttt ctgggcttcc attggtttat ctgtatcacc gcttccgttg ggaagtggaa
    20461 tgcagtatga gagctcggtt tctcttggat acttaaatca atcgtttcaa aatgcagtta
    20521 tggaggggat acgctatggc tgtgaacaag gattgtatgg ttggaatgtg acggactgta
    20581 aaatctgttt taagtatggc ttatactata gccctgttag taccccagca gattttcgga
    20641 tgcttgctcc tattgtattg gaacaagtct taaaaaaagc tggaacagaa ttgttagagc
    20701 catatcttag ttttaaaatt tatgcgccac aggaatatct ttcacgagca tacaacgatg
    20761 ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa aaataatgag gtcattctta
    20821 gtggagaaat ccctgctcgg tgtattcaag aatatcgtag tgatttaact ttctttacaa
    20881 atggacgtag tgtttgttta acagagttaa aagggtacca tgttactacc ggtgaacctg
    20941 tttgccagcc ccgtcgtcca aatagtcgga tagataaagt acgatatatg ttcaataaaa
    21001 taacttagtg tattttatgt tgttatataa atatggtttc ttgttaaata agatgaaata
    21061 ttttttaata aagatttgaa ttaaagtgta aaggaggaga tagttattat aaactacaag
    21121 tggatattgt gtcctgtatg tggaaataaa acacgattaa agataaggga agatactgaa
    21181 ttaaaaaaat tccccctcta ttgtccgaaa tgcagacaag aaaatttaat tgaaataaag
    21241 cagttcaaag taactgtgat tacagagcca gacgcaaaga cgcagagccg ataaaatgag
    21301 attaatacaa tctcatttta tcggctcttt ccgttatgta tggattcttt taattagtct
    21361 tcgatgtttc ttgcttcgtt gataccgctg gctaaagatt ccattaagga tagttctttg
    21421 tctgtaaagc tatccatgta tttctctatc tgtaatcgtc gggtgctttt taccaagtta
    21481 ttagcaggta agaaaaattc atcaacggaa acatgaagta acgatacaag gtcataaaga
    21541 acttgtatgc tggggtgttg ccctttattt tcaatattag ttaagtaccg tgggtcaatt
    21601 tcaatcaatg ctcccacttg ttcacgagtt aaacctcgtt tcaatcgagc ttctttaatg
    21661 gctaaaccaa aggctctaaa atcatattta tcttcttttt tacgcatagt agaccacctc
    21721 tatacatttt attgttccta ctgaattaaa aacaggtata gaaaaacgtg ttatatggtt
    21781 tataggttta tatttaataa aaagcactac taaacgccaa taaaaaaaac cgttatatgg
    21841 tagtgctatt tacgctgtta aaatattgta tattacttcc aaatggcggt ttgttggagg
    21901 tcaacgtcgc catgaagtac atcatataca ataaatttcc ttacattggg ttcttgtcaa
    21961 aaaaagtcgt ctatctgcaa tagataagta cgtccaccaa tgtggtttta taaatcatat
    22021 agatagaata acagaagcat gtaaacagag aaataaatct gtttatatgc ttttttggct
    22081 attcagaact tttttacaaa gtttatttat cagtaatgca acaaatcccc ctttcacatt
    22141 gggactaaga gtgaaaggag ataaacgagc aaggctcact tcctttccta gacagaaagg
    22201 gggtgagatt atgaggacat cttctttcga gcatatcgtc agattacagt tcaattcttt
    22261 aatgctgatt gtcattaaga acaaagtgaa aagtcgaaac agacagcttg caagacattc
    22321 taaacgtgaa gtattgtttt gtgaaatagc cgaaacgaaa caagttgaac gtggaacgat
    22381 tgatacctgt ttttatgatt gtgtatcatt taaagtcttg cactttacaa tttatgtatc
    22441 tgatgaaaca cttggtacag ttttacatag actatcagaa aaacagcgta gtgcgatttt
    22501 attacgttat tttcaaggaa tgaacgacag agaaatttca gaactgtacc atgtatcacg
    22561 ttctgctatt tgcagtagaa gaactagagg actaaggaag ttaaaaatgc ttttgagcga
    22621 aaggaagtaa agattatggc tatccctctt ttgaattgat ttgcaaagcg tcaagcggag
    22681 atgaaatggc agttaaagag attttgaagt tttatgacgg ttatatttct aaattatgct
    22741 tgcgtccttt ttaccactct gaaagtggta aaatcattat gcaggttgat gaagaactaa
    22801 aaggagaaat tcatacagac atgatgaaag caatattgaa atttgaaatc ggagtgaagt
    22861 aatcaactgt ataaaaaaat cgggagatac acagtttcgt gttatctccc ttagtagttt
    22921 tacggtcata tcaaggctca ttcaatcgtg gtaaattcgt ggtaagatga gtgtttttct
    22981 atacttcaaa atgcttgaat gtatagtgtt tatgcggata attgaaaatt agatataaaa
    23041 tttaataaaa accaaaaatg cacggtcgcc atgaagggac cgtgtatttt tatcttctta
    23101 ttttctcacc taatcgtatc agccgcatcg cttgttttcc tgcacaaaca agcaatcgct
    23161 ctacctgttc catcgcataa gaaagtacca tcggttcatt gatgatatca agtacatagt
    23221 cgataccacc ctcacttaat tgagcttgat ttttcttctc catattatgt agttgtggga
    23281 caactttctg acaatcagca attgtgtcaa gaacggatca atcctttatt ggagttgctc
    23341 gaaagggtct tctcttatta cggacaggct ttgctcactg tacatagaca acaaatcatt
    23401 atcctagtca atcggatttc cagagcctca ttgctatcat tgcttgataa aatccagaca
    23461 aagtttaata aaatgtatca gttacagcta aactttggaa ttgggtcgct ttgttataca
    23521 gagcaagaga caccgcaatc tttccttcat gccaaacaag tttgcgaatg gatagctttc
    23581 catcaatctg tgaacgagat ccgctttttt gaagatttag acctcgggat cgttttacca
    23641 gcaa
//
LOCUS       DANKBF010000236        13213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.079, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000236 DANKBF010000000
VERSION     DANKBF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(204..2162)
                     /locus_tag="PWY89_003117"
     CDS             complement(204..2162)
                     /locus_tag="PWY89_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2068050.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="PWY89_003118"
     CDS             complement(2159..3031)
                     /locus_tag="PWY89_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2068051.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="PWY89_003119"
     CDS             complement(3373..4245)
                     /locus_tag="PWY89_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2068052.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="PWY89_003120"
     CDS             4373..4942
                     /locus_tag="PWY89_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2068053.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="PWY89_003121"
     CDS             5009..5746
                     /locus_tag="PWY89_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2068054.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="PWY89_003122"
     CDS             5977..7245
                     /locus_tag="PWY89_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2068055.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="PWY89_003123"
     CDS             7732..8196
                     /locus_tag="PWY89_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2068056.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="PWY89_003124"
     CDS             8180..8809
                     /locus_tag="PWY89_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2068057.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="PWY89_003125"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="PWY89_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2068058.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="PWY89_003126"
     CDS             10688..11134
                     /locus_tag="PWY89_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2068059.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="PWY89_003127"
     CDS             complement(11333..11848)
                     /locus_tag="PWY89_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2068060.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="PWY89_003128"
     CDS             11965..13167
                     /locus_tag="PWY89_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2068061.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DANKBF010000237        12769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.081, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000237 DANKBF010000000
VERSION     DANKBF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            complement(134..577)
                     /locus_tag="PWY89_003129"
     CDS             complement(134..577)
                     /locus_tag="PWY89_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2068062.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="PWY89_003130"
     CDS             complement(795..2135)
                     /locus_tag="PWY89_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2068063.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="PWY89_003131"
     CDS             2404..3300
                     /locus_tag="PWY89_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2068064.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="PWY89_003132"
     CDS             complement(3450..4391)
                     /locus_tag="PWY89_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2068065.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="PWY89_003133"
     CDS             4517..5638
                     /locus_tag="PWY89_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2068066.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="PWY89_003134"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="PWY89_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2068067.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="PWY89_003135"
     CDS             7426..7992
                     /locus_tag="PWY89_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2068068.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="PWY89_003136"
     CDS             8203..9264
                     /locus_tag="PWY89_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2068069.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="PWY89_003137"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="PWY89_003137"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2068070.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="PWY89_003138"
     CDS             10177..12159
                     /locus_tag="PWY89_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2068071.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="PWY89_003139"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="PWY89_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2068072.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         4200 a         2333 c         2650 g         3586 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgag
//
LOCUS       DANKBF010000238         5731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.115, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000238 DANKBF010000000
VERSION     DANKBF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            202..894
                     /locus_tag="PWY89_003140"
     CDS             202..894
                     /locus_tag="PWY89_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2068073.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1221..1718
                     /gene="dfrG"
                     /locus_tag="PWY89_003141"
     CDS             1221..1718
                     /gene="dfrG"
                     /locus_tag="PWY89_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2068074.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1790..3742)
                     /locus_tag="PWY89_003142"
     CDS             complement(1790..3742)
                     /locus_tag="PWY89_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2068075.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3749..3985)
                     /locus_tag="PWY89_003143"
     CDS             complement(3749..3985)
                     /locus_tag="PWY89_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068076.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4387..4677
                     /locus_tag="PWY89_003144"
     CDS             4387..4677
                     /locus_tag="PWY89_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2068077.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            5391..5693
                     /locus_tag="PWY89_003145"
                     /pseudo
     CDS             5391..5693
                     /locus_tag="PWY89_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1955 a          912 c          991 g         1873 t
ORIGIN      
        1 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
       61 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      121 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      181 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      241 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      301 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      361 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      421 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      481 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      541 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      601 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      661 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      721 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      781 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      841 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
      901 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
      961 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1021 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1081 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1141 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1201 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1261 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1321 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1381 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1441 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1501 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1561 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1621 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1681 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1741 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1801 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1861 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     1921 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     1981 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2041 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2101 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2161 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2221 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2281 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2341 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2401 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2461 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2521 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2581 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2641 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2701 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2761 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2821 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     2881 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     2941 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3001 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3061 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3121 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3181 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3241 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3301 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3361 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3421 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3481 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3541 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3601 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3661 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3721 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3781 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3841 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     3901 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     3961 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4021 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4081 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4141 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4201 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4261 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4321 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4381 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4441 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4501 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4561 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4621 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4681 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4741 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4801 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4861 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     4921 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     4981 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5041 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5101 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5161 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5221 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5281 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5341 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5401 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5461 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5521 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5581 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5641 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5701 taaaatattt tgtgtaaatg aaaaaatcca t
//
LOCUS       DANKBF010000239         4802 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.133, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000239 DANKBF010000000
VERSION     DANKBF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            90..785
                     /gene="vanR"
                     /locus_tag="PWY89_003146"
     CDS             90..785
                     /gene="vanR"
                     /locus_tag="PWY89_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL2068078.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="PWY89_003147"
     CDS             763..1917
                     /locus_tag="PWY89_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2068079.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="PWY89_003148"
     CDS             2132..3100
                     /locus_tag="PWY89_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL2068080.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="PWY89_003149"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="PWY89_003149"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL2068081.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="PWY89_003150"
     CDS             4130..4738
                     /locus_tag="PWY89_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL2068082.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DANKBF010000240         3010 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.149, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000240 DANKBF010000000
VERSION     DANKBF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            260..1171
                     /locus_tag="PWY89_003151"
     CDS             260..1171
                     /locus_tag="PWY89_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDL2068083.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ"
                     /locus_tag="PWY89_003152"
     CDS             1324..1809
                     /gene="vanZ"
                     /locus_tag="PWY89_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDL2068084.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="PWY89_003153"
     CDS             2328..2612
                     /locus_tag="PWY89_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2068085.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT         1105 a          435 c          556 g          914 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa
//
LOCUS       DANKBF010000241         2771 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.151, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000241 DANKBF010000000
VERSION     DANKBF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            110..193
                     /locus_tag="PWY89_003154"
     CDS             110..193
                     /locus_tag="PWY89_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2068086.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="PWY89_003155"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="PWY89_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2068087.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="PWY89_003156"
     CDS             complement(1318..2181)
                     /locus_tag="PWY89_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2068088.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="PWY89_003157"
     CDS             complement(2183..2455)
                     /locus_tag="PWY89_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2068089.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="PWY89_003158"
     CDS             complement(2472..2681)
                     /locus_tag="PWY89_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2068090.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          990 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattaa
     2761 tcacttgtga t
//
LOCUS       DANKBF010000242         2325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.157, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000242 DANKBF010000000
VERSION     DANKBF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..96
                     /locus_tag="PWY89_003159"
     CDS             <1..96
                     /locus_tag="PWY89_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2068091.1"
                     /translation="MTEKLYKEVNEIVSEINNFLNEESSDERKQI"
     gene            77..811
                     /locus_tag="PWY89_003160"
     CDS             77..811
                     /locus_tag="PWY89_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2068092.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            844..1752
                     /locus_tag="PWY89_003161"
     CDS             844..1752
                     /locus_tag="PWY89_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2068093.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1749..2291
                     /gene="sat4"
                     /locus_tag="PWY89_003162"
     CDS             1749..2291
                     /gene="sat4"
                     /locus_tag="PWY89_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL2068094.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
BASE COUNT          817 a          332 c          526 g          650 t
ORIGIN      
        1 atgaccgaga aactttataa agaagtgaat gaaattgtat cggagataaa taacttttta
       61 aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt ttcaaaaata
      121 cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg agactttgaa
      181 aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg gctatggatg
      241 gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg atatttctca
      301 taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat atgaatgctg
      361 tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa gttcgcttgc
      421 gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga tgctgaaggc
      481 tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg gaacacaaga
      541 ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt attattatga
      601 gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata gaacactgac
      661 cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg tggagccaca
      721 gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac gcccaatgat
      781 gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac gaggagtatg
      841 taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga acaggatgaa
      901 cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc taaagatgaa
      961 tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat atctaatgat
     1021 gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga tatggaatta
     1081 ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta caataaaatt
     1141 gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga taaattaata
     1201 aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac tgatatagat
     1261 tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga attttggaat
     1321 gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc tattgatcat
     1381 tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt cggcatcgaa
     1441 acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta tatatccgag
     1501 gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa catatgggaa
     1561 gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc ggaaaggctt
     1621 cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga catgtataaa
     1681 aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat agaagagagg
     1741 cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat aaacccagcg
     1801 aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta
     1861 cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg
     1921 atgaggaggc agattgcctt gaatatattg acaatactga taagataata tatctttact
     1981 accaagacga taaatgcgtc ggaaaagtta aactgcgaaa aaattggaac cggtacgctt
     2041 atatagaaga tatcgccgta tgtaaggatt tcagggggca aggcataggc agcgcgctta
     2101 tcaatatatc tatagaatgg gcaaagcata aaaacttgca tggactaatg cttgaaaccc
     2161 aggacaataa ccttatagct tgtaaattct atcataattg tggtttcaaa atcggctccg
     2221 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt
     2281 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgt
//
LOCUS       DANKBF010000243         2263 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.159, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000243 DANKBF010000000
VERSION     DANKBF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            <1..96
                     /locus_tag="PWY89_003163"
     CDS             <1..96
                     /locus_tag="PWY89_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2068095.1"
                     /translation="MTEKLYKEVNEIVSEINNFLNEESSDERKQI"
     gene            77..811
                     /locus_tag="PWY89_003164"
     CDS             77..811
                     /locus_tag="PWY89_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2068096.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            844..1752
                     /locus_tag="PWY89_003165"
     CDS             844..1752
                     /locus_tag="PWY89_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2068097.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1749..2229
                     /locus_tag="PWY89_003166"
                     /pseudo
     CDS             1749..2229
                     /locus_tag="PWY89_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          792 a          320 c          512 g          639 t
ORIGIN      
        1 atgaccgaga aactttataa agaagtgaat gaaattgtat cggagataaa taacttttta
       61 aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt ttcaaaaata
      121 cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg agactttgaa
      181 aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg gctatggatg
      241 gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg atatttctca
      301 taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat atgaatgctg
      361 tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa gttcgcttgc
      421 gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga tgctgaaggc
      481 tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg gaacacaaga
      541 ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt attattatga
      601 gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata gaacactgac
      661 cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg tggagccaca
      721 gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac gcccaatgat
      781 gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac gaggagtatg
      841 taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga acaggatgaa
      901 cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc taaagatgaa
      961 tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat atctaatgat
     1021 gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga tatggaatta
     1081 ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta caataaaatt
     1141 gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga taaattaata
     1201 aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac tgatatagat
     1261 tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga attttggaat
     1321 gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc tattgatcat
     1381 tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt cggcatcgaa
     1441 acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta tatatccgag
     1501 gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa catatgggaa
     1561 gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc ggaaaggctt
     1621 cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga catgtataaa
     1681 aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat agaagagagg
     1741 cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat aaacccagcg
     1801 aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta
     1861 cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg
     1921 atgaggaggc agattgcctt gaatatattg acaatactga taagataata tatcttttat
     1981 atagaagata tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc
     2041 aatatatcta tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag
     2101 gacaataacc ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc
     2161 gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta
     2221 aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgt
//
LOCUS       DANKBF010000244         2026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.163, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000244 DANKBF010000000
VERSION     DANKBF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            99..893
                     /locus_tag="PWY89_003167"
                     /note="aph(3')-IIIa"
     CDS             99..893
                     /locus_tag="PWY89_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL2068098.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1169..1741
                     /locus_tag="PWY89_003168"
     CDS             1169..1741
                     /locus_tag="PWY89_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2068099.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT          681 a          325 c          465 g          555 t
ORIGIN      
        1 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
       61 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
      121 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
      181 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
      241 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
      301 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
      361 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
      421 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
      481 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
      541 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
      601 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
      661 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
      721 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
      781 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
      841 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
      901 atttagatgt ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt
      961 acatccgcaa ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagataga
     1021 gttctatatt agagatgtaa agagttttgg tgaagaggtt tgtgagtatg gcttttataa
     1081 ccagggtgca atgagaagca ctaacagcag ctagcttaag aacgcatagg atatactttt
     1141 tatggaagtc acagaggagg aataaaattt gaacaggatt aacagagtaa cctctattct
     1201 tattcagctg caatcaaaaa aaataattcc tgccaaagaa attgcacagc gatttaatat
     1261 aagcttaagg acagtttaca gagatatccg gacacttgaa gaagccggaa taccaattgg
     1321 atctgaggcc ggaaaaggat attttcttgt agagggcttc ctacttccgc cggtaatgtt
     1381 tacagcggcg gaagtgggtg cattgattac agcagggaaa tttttaaatt gccatggaga
     1441 tgaatcattt ataaaggatt ttgattcagc tatgtataaa atcaaatcta ttttgaagca
     1501 tggtgaaaaa aactatgcac aggagttgga gaacagtatt aacgtgtaca gcacatctgg
     1561 acagaaaaat acattggcgg ataacgttat tgcagcaatt cagactgcaa tctgcaataa
     1621 aagggtaata tcaattcaat atcctgcatc aggagggcaa gaaccggaaa gcagaatgat
     1681 agagcctata tcactggggt tttatgaaca gaactggtac ttaatcggtt ttgcaggcta
     1741 agaaatgaat ataggaattt tcgtgtggat cggattaaaa gcatttgcat agaagaagag
     1801 ctctgtcaaa gccatgatgg ctctttggaa caaatattaa aacaaatgct ttcatataaa
     1861 aaactatatg ccctacaatg ccgttgtaaa aattggggaa aagggatata ttgattttat
     1921 atctttctca aaataaatat aaaagccctt taaagagggc ttttatatat taatcacaaa
     1981 tcacttatca caaatcacaa gtgattaatc acaaatcact tgtgat
//
LOCUS       DANKBF010000245          871 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538773-rid17233683.guided.209, whole genome shotgun
            sequence.
ACCESSION   DANKBF010000245 DANKBF010000000
VERSION     DANKBF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538773
            Sequence Read Archive: ERR10688336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638098.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638098.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,107
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 33 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538773-rid17233683.guided.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-05-04"
     gene            155..703
                     /gene="aac(6')"
                     /locus_tag="PWY89_003169"
     CDS             155..703
                     /gene="aac(6')"
                     /locus_tag="PWY89_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2068100.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          312 a          127 c          192 g          240 t
ORIGIN      
        1 tgaataaagt gtttctgtat aattttttta ttttataagt aagataaata atgaaagaat
       61 agagatactg attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga
      121 ctgttagaat aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa
      181 taatccagta ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata
      241 tggagacagc tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc
      301 agcggtagac caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac
      361 aggttgggaa ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac
      421 tcgattagtc aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt
      481 aggtacggat gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac
      541 atttgataaa gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa
      601 attaggttat aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat
      661 ttggatggca aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac
      721 agaagtaatc agcttcaaga aataagaagg aatttacgaa aattacttct tgaatttttg
      781 tgaaattcca acttatttcc gaagaagcta cttttggaac accgtgtacc agaggttgtg
      841 aaaaagtcgc ttagtttcga gtagtaagaa a
//
